SMED30025466
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30025466 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Alignments
SMED30025466 aligns in the following genomic locations:
Homology
BLAST of SMED30025466 vs. Ensembl Human
Match: MTX2 (metaxin 2 [Source:HGNC Symbol;Acc:HGNC:7506]) HSP 1 Score: 48.9062 bits (115), Expect = 4.629e-6 Identity = 40/168 (23.81%), Postives = 74/168 (44.05%), Query Frame = 1 Query: 247 NVEHVSNDGIYPLFSIDSKLYSGIENAKSCIEDYNNRTSMNDQNIQGKTLVERIQFHNEIILFNYLKSFFENIYLYEQWMSEGVRQ--TKSNFIKRYTWPLNRILWLIKERAVKNFM-TLSYFKESKQDLVKEFEVLCTHLNNNI-EDTFLFNKSTPHELDLLVYGFI 738 N E++S G P + +++ S + ++ + S + +Q + ++ N ++L LY QW E T + + Y WPLN IL K+ VK M + + K++ ++++ + C L+ + + FNK P ELD LV+G + Sbjct: 62 NAEYMSPSGKVPFIHVGNQVVSELGPIVQFVKAKGHSLSDGLEEVQKAEMKAYMELVNNMLL---------TAELYLQWCDEATVGEITHARYGSPYPWPLNHILAYQKQWEVKRKMKAIGWGKKTLDQVLEDVDQCCQALSQRLGTQPYFFNKQ-PTELDALVFGHL 219
BLAST of SMED30025466 vs. TrEMBL
Match: A0A068WC77 (Metaxin 2 OS=Echinococcus granulosus OX=6210 GN=EgrG_000759400 PE=4 SV=1) HSP 1 Score: 88.5817 bits (218), Expect = 9.354e-17 Identity = 58/229 (25.33%), Postives = 105/229 (45.85%), Query Frame = 1 Query: 163 LPLEKAQIFTIASFFLAKQIKFQFEQINNVEHVSNDGIYPLFSIDSKLYSGIENAKSCIEDYNNRTSMNDQNIQGKTLVERIQFHNEIILFNYLKSFFENIYLYEQWMSE-GVRQTKSNFIKRYTWPLNRILWLIKERAVKNFMTLS-YFKESKQDLVKEFEVLCTHLNNNIED-TFLFNKSTPHELDLLVYGFITVLQHYPNYFETYIGIISKFPKINQLLLRINVIN 840 LP ++A ++FL K I F+ + + S DG+ PL +I L SG + ++ +N + N+ + K L + H I F ++ N+ LY W+ G R T+ F Y WPL +++ + F+T + + + D++K F+ C H++ I D F+FN+ ++D L+ + YF IISK+P + +L + ++ Sbjct: 15 LPNDEATTAACLTYFLLKSIPFKVRYLRDAYFASPDGLLPLSNICGNLVSGFMSMVFTLKTFNGK---NEVELDAKVLEMKNTHH---IFFFWVADTLRNLTLYLTWVEPNGERHTRDRFNSMYPWPLGDYYCNRQKKLILKFLTSADWASDGLGDVLKRFDTTCKHISCMITDGPFVFNRKKCGKVDCLIAAYFNTFSTNAKYFAPLTPIISKYPSLTKLATSVRSLH 237
BLAST of SMED30025466 vs. TrEMBL
Match: A0A3P7LSM5 (Thioredoxin-like_fold domain-containing protein OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS9794 PE=4 SV=1) HSP 1 Score: 88.1965 bits (217), Expect = 1.658e-16 Identity = 51/223 (22.87%), Postives = 118/223 (52.91%), Query Frame = 1 Query: 160 NLPLEKAQIFTIASFFLAKQIKFQFEQINNVEHVSNDGIYPLFSIDSKLYSGIENAKSCIEDYNNRTSMNDQNIQGKTLVERIQFHNEIILFNYLKSFFENIYLYEQWM-SEGVRQTKSNFIKRYTWPLNRILWLIKERAVKNFM-TLSYFKESKQDLVKEFEVLCTHLNNNIE-DTFLFNKSTPHELDLLVYGFITVLQHYPNYFETYIGIISKFPKINQLL 819 +LP ++A ++ K I F ++++N +S DG+ P+ +I +L SG + I++ + + N++ K V + + + + F +L NI LY W+ G++ TK+ K + WP+ +++ ++ A + +M + + ++++EF+ +C+++ + D F+F+++ P ++D L+ ++ + + Y F I ++SK+P + QL+ Sbjct: 17 SLPNDEATTNACKTYLKLKGISFLCQELSNAYTISPDGLLPVLNIHGRLVSGFMSMCLAIKE-----TKKNPNLELKPEVLQTKNCHHVFFF-WLSDALRNIALYFTWVNPSGIQHTKNKLQKAFPWPICNVMFNRQKLAYEKYMGAIGWLSREPGEIIEEFDRVCSNVTKVLTNDHFVFDRTKPGKIDCLLCSYLMMFEKYHVLFSPLISVLSKYPALRQLV 233
BLAST of SMED30025466 vs. TrEMBL
Match: A0A0R3W2R9 (Thioredoxin-like_fold domain-containing protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS4151 PE=4 SV=1) HSP 1 Score: 87.0409 bits (214), Expect = 3.694e-16 Identity = 55/225 (24.44%), Postives = 104/225 (46.22%), Query Frame = 1 Query: 163 LPLEKAQIFTIASFFLAKQIKFQFEQINNVEHVSNDGIYPLFSIDSKLYSGIENAKSCIEDYNNRTSMNDQNIQGKTLVERIQFHNEIILFNYLKSFFENIYLYEQWM-SEGVRQTKSNFIKRYTWPLNRILWLIKERAVKNFMTLS-YFKESKQDLVKEFEVLCTHLNNNIED-TFLFNKSTPHELDLLVYGFITVLQHYPNYFETYIGIISKFPKINQLLLRI 828 LP ++A + ++K I F + N S DG+ PL +I L SG + ++ ++ ++ D + K L + H I F ++ F N+ LY W+ G R T+ +F + WPL + +++ + F+T + + E D++ F+ C H++ + D F+FN+ ++D L+ + YF IISK+P + +L+ + Sbjct: 15 LPNDEATTAACLKYLISKSIPFSIRYLKNAYFASPDGLLPLSNICGNLVSGFMSMVFTLKTFSRKS---DVELDSKVLETKNTRH---IFFFWIADVFRNVTLYLTWVEPNGQRHTRGHFNSTHPWPLGDYYFNRQKKLIMRFLTSANWASEDLGDVLNRFDATCKHVSCMLTDGPFVFNRKKAGKVDCLLAAYFNTFSANAKYFAVLAPIISKYPSLTRLVASV 233
BLAST of SMED30025466 vs. TrEMBL
Match: A0A068Y9U9 (Metaxin 2 OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000759400 PE=4 SV=1) HSP 1 Score: 86.2705 bits (212), Expect = 6.152e-16 Identity = 56/229 (24.45%), Postives = 106/229 (46.29%), Query Frame = 1 Query: 163 LPLEKAQIFTIASFFLAKQIKFQFEQINNVEHVSNDGIYPLFSIDSKLYSGIENAKSCIEDYNNRTSMNDQNIQGKTLVERIQFHNEIILFNYLKSFFENIYLYEQWMSE-GVRQTKSNFIKRYTWPLNRILWLIKERAVKNFMTLS-YFKESKQDLVKEFEVLCTHLNNNIED-TFLFNKSTPHELDLLVYGFITVLQHYPNYFETYIGIISKFPKINQLLLRINVIN 840 LP ++A ++ L K I F+ + + S DG+ PL +I L SG + ++ +N + N+ + K L + H I F ++ N+ LY W+ G R T+ +F Y WPL + +++ + F+T + + + D++K F+ C H++ + D F+FN+ ++D L+ + YF IISK+P + +L + ++ Sbjct: 15 LPNDEATTAACLTYLLLKSIPFKVRYLRDAYFASPDGLLPLSNIRGNLVSGFMSMVFTLKTFNGK---NEVELDAKVLEIKNTHH---IFFFWVADTLRNLTLYLTWVEPNGERHTRDHFNSMYPWPLGDYYFNRQKKLILKFLTSADWASDGLGDILKRFDTTCKHISCMLTDGPFVFNRKKGGKVDCLIAAYFNTFSTNAKYFAPLTPIISKYPSLTKLATSVRSLH 237
BLAST of SMED30025466 vs. TrEMBL
Match: A0A0X3P3M4 (Metaxin-2 OS=Schistocephalus solidus OX=70667 GN=MTX2 PE=4 SV=1) HSP 1 Score: 85.8853 bits (211), Expect = 1.095e-15 Identity = 51/231 (22.08%), Postives = 116/231 (50.22%), Query Frame = 1 Query: 136 IIYAERLQNLPLEKAQIFTIASFFLAKQIKFQFEQINNVEHVSNDGIYPLFSIDSKLYSGIENAKSCIEDYNNRTSMNDQNIQGKTLVERIQFHNEIILFNYLKSFFENIYLYEQWM-SEGVRQTKSNFIKRYTWPLNRILWLIKERAVKNFM-TLSYFKESKQDLVKEFEVLCTHLNNNIE-DTFLFNKSTPHELDLLVYGFITVLQHYPNYFETYIGIISKFPKINQLL 819 ++ + R +LP + A ++ K I ++ N VS DG+ P+ +I +L SG + I + + + N++ K+ + + + + + F +L NI +Y W+ G+++TK K + WP+ I++ ++ A + +M + + ++V+EF+ +C+ ++ + D F+F + P ++D ++ + + YP +F +G++S +P + QL+ Sbjct: 9 LVMSPRAFSLPNDIATTEACRTYLKLKGISVSCRELPNAYAVSPDGLLPVLNIHGRLVSGFMSMCLAI-----KATKKNPNLELKSEILQAKNSHHVFFF-WLSDALRNIAIYFSWVNPSGLQRTKYILQKAFPWPVCNIIFNRQKLAYEKYMEAIGWLSRETGEIVEEFDRVCSKVSKVLTNDHFVFERKKPGKIDCILCSYFVTFEKYPIFFSPLLGVMSNYPALRQLV 233
BLAST of SMED30025466 vs. Ensembl Nematostella
Match: EDO34336 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SPN8]) HSP 1 Score: 53.9138 bits (128), Expect = 1.853e-8 Identity = 52/217 (23.96%), Postives = 98/217 (45.16%), Query Frame = 1 Query: 190 TIASFFLAKQIKFQFEQINNVEHVSNDGIYPLFSIDSKLYSGIENAKSCIEDYNNRTSMNDQNIQGKTLVERIQFHNEIILFNYLKSFFENIYLYEQWMSEGVR-QTKSNFIKRYTWPLNRILWLIKERAV-KNFMTLSYFKESKQDLVKEFEVLCTHLNNNIEDTFLFNKSTPHELDLLVYGFI-TVLQHY-PNYFETYIGIISKFPKINQLLLRI 828 ++ +F Q+ + + + N E +S G PL L G ++ S + N++ Q+ + + E + I L N +S LY W G + +T + ++ PLN IL K+ A ++ L++ ++ + +V+EFE C L + F + P E+D L +G I ++L Y P+ +T + I+ K + ++RI Sbjct: 31 SLIAFLKFCQLPYDLKCVKNAESMSPTGEVPLLRAGKDLVGGPQDIFSLV---NHKGLSVSQDFEARDKAEMKAY---ISLIN--RSILPT-ELYMLWCKPGWQIRTTHQYGSQFPVPLNYILSYRKQWAAHRSLHALNWQRKKEAGVVEEFEKACQALAEKLGTNQYFIQGRPTEVDALAFGHINSILSRYQPD--DTLVAIVHKHRNLEDFVMRI 236
BLAST of SMED30025466 vs. Planmine SMEST
Match: SMESG000065449.1 (SMESG000065449.1) HSP 1 Score: 515.383 bits (1326), Expect = 0.000e+0 Identity = 253/254 (99.61%), Postives = 254/254 (100.00%), Query Frame = 1 Query: 121 MSSKVIIYAERLQNLPLEKAQIFTIASFFLAKQIKFQFEQINNVEHVSNDGIYPLFSIDSKLYSGIENAKSCIEDYNNRTSMNDQNIQGKTLVERIQFHNEIILFNYLKSFFENIYLYEQWMSEGVRQTKSNFIKRYTWPLNRILWLIKERAVKNFMTLSYFKESKQDLVKEFEVLCTHLNNNIEDTFLFNKSTPHELDLLVYGFITVLQHYPNYFETYIGIISKFPKINQLLLRINVINSDILGFWKKHLFNL 882 MSSKVIIYAERLQNLPLEKAQIFTIASFFLAKQIKFQFEQINNVEHVSNDGIYPLFSIDSKLYSGIENAKSCIEDYNNRTSM+DQNIQGKTLVERIQFHNEIILFNYLKSFFENIYLYEQWMSEGVRQTKSNFIKRYTWPLNRILWLIKERAVKNFMTLSYFKESKQDLVKEFEVLCTHLNNNIEDTFLFNKSTPHELDLLVYGFITVLQHYPNYFETYIGIISKFPKINQLLLRINVINSDILGFWKKHLFNL Sbjct: 1 MSSKVIIYAERLQNLPLEKAQIFTIASFFLAKQIKFQFEQINNVEHVSNDGIYPLFSIDSKLYSGIENAKSCIEDYNNRTSMSDQNIQGKTLVERIQFHNEIILFNYLKSFFENIYLYEQWMSEGVRQTKSNFIKRYTWPLNRILWLIKERAVKNFMTLSYFKESKQDLVKEFEVLCTHLNNNIEDTFLFNKSTPHELDLLVYGFITVLQHYPNYFETYIGIISKFPKINQLLLRINVINSDILGFWKKHLFNL 254 The following BLAST results are available for this feature:
BLAST of SMED30025466 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 1
BLAST of SMED30025466 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30025466 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30025466 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30025466 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30025466 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30025466 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30025466 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30025466 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30025466 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30025466 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30025466 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of SMED30025466 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30025466 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30025466 ID=SMED30025466|Name=SMED30025466|organism=Schmidtea mediterranea sexual|type=transcript|length=1025bpback to top protein sequence of SMED30025466-orf-1 >SMED30025466-orf-1 ID=SMED30025466-orf-1|Name=SMED30025466-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=255bp MSSKVIIYAERLQNLPLEKAQIFTIASFFLAKQIKFQFEQINNVEHVSNDback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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