tRNA-splicing endonuclease subunit Sen34
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30025404 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Homology
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. Ensembl Fly
Match: CG33260 (gene:FBgn0053260 transcript:FBtr0302436) HSP 1 Score: 65.855 bits (159), Expect = 3.082e-11 Identity = 38/105 (36.19%), Postives = 57/105 (54.29%), Query Frame = 3 Query: 1803 VKTAVFYNLWSRGHCVTVSCKKMGGDYLLYPGDPWFHHATHVVRILPQTLEIKPSQLISKIRLINSLKKTYLIAWYDDNAVAKSSNLSSIVEHLNISFNCLGWCG 2117 +K VF +LW RG VT S G D+L+YPGDP +HA+H+V IL + IKP +LI+K+RL + K+ + A+ +N + I + + WC Sbjct: 183 LKYRVFKDLWRRGKYVT-SGDAFGADFLVYPGDPMIYHASHIV-ILHEQPVIKPLELIAKVRLSVIVNKSCVFAYEMENNEEE------------IHYQTVAWCN 273
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. Ensembl Zebrafish
Match: tsen34 (TSEN34 tRNA splicing endonuclease subunit [Source:ZFIN;Acc:ZDB-GENE-050419-115]) HSP 1 Score: 51.9878 bits (123), Expect = 1.766e-6 Identity = 31/93 (33.33%), Postives = 53/93 (56.99%), Query Frame = 3 Query: 1785 LPLD--LVVKTAVFYNLWSRGHCVTVSCKKMGGDYLLYPGDPWFHHATHVVRILPQTLEIKPSQLISKIRLINSLKKTYLIAWYDDNAVAKSS 2057 +P D L + +V+ +L ++G+ +T S K GGD+L+YPGDP HA ++ + E+ +++ RL +++KKT L+ D V SS Sbjct: 210 MPRDEHLNTRFSVYRDLRNKGYYLT-SAGKFGGDFLVYPGDPLRFHAHYIALCVSLDEEMPVCDILAIARLGSNVKKTVLLCSPQDGDVVYSS 301
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. Ensembl Zebrafish
Match: tsen34 (TSEN34 tRNA splicing endonuclease subunit [Source:ZFIN;Acc:ZDB-GENE-050419-115]) HSP 1 Score: 51.9878 bits (123), Expect = 1.766e-6 Identity = 31/93 (33.33%), Postives = 53/93 (56.99%), Query Frame = 3 Query: 1785 LPLD--LVVKTAVFYNLWSRGHCVTVSCKKMGGDYLLYPGDPWFHHATHVVRILPQTLEIKPSQLISKIRLINSLKKTYLIAWYDDNAVAKSS 2057 +P D L + +V+ +L ++G+ +T S K GGD+L+YPGDP HA ++ + E+ +++ RL +++KKT L+ D V SS Sbjct: 210 MPRDEHLNTRFSVYRDLRNKGYYLT-SAGKFGGDFLVYPGDPLRFHAHYIALCVSLDEEMPVCDILAIARLGSNVKKTVLLCSPQDGDVVYSS 301
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. Ensembl Xenopus
Match: TSEN34 (tRNA splicing endonuclease subunit 34 [Source:NCBI gene;Acc:100380139]) HSP 1 Score: 88.1965 bits (217), Expect = 2.891e-18 Identity = 81/327 (24.77%), Postives = 144/327 (44.04%), Query Frame = 3 Query: 1224 VWDPDSVRFLRDECRIFGKFIGSVARLPHKTVFKGLPLMLAVEEF--------VVLLKEHSERVRLVRSISL---VSPLIPESSSASVDQYKSDIEHNY-NEISDIIQEMRLDRSNLHFDSVGDSQIQGRKRK----ADGARVSIAPSVAP-----DLEK--------VSVHLPTSTPTDWLRVGEKETIDIPKLLSLAEVTDFVISLLQRKFEQKLPLDLVVKTAVFYNLWSRGHCVTVSCKKMGGDYLLYPGDPWFHHATHVVRILPQTLEIKPSQLISKIRLINSLKKTYLIAWYDDNAVAKSSNLSSIVEHLNISFNCLGWCG 2117 VW D V+ +R++ + G +G++ R P + GLPL L EE VL++ S ++ + ++L + P + + +Q +++ Y NE +++ L+ +S+ D +GR R+ +D + + P +LE+ + VHLPT+ P+ G+ E +D + + + Q++ E V+ VF +LW +G+ +T S K GGD+L+YPGDP HA + P EI S +++ RL ++KKT L+ D+ ++F L W G Sbjct: 13 VWKADDVQMIREQHGLVGNLVGALVRKPRQNSRLGLPLQLMPEEVRLLVEIGAAVLVRGLSGEGKIHKEVALEVESAESSPSPNDVNDEQPETEAYDKYLNESYKEQRKLALEDKRRTLESLADRIAEGRSRRKRQRSDQEDATESSFQGPIKELQNLEETFQFPKEAMMVHLPTAHPS----CGDTEEVD------MCQASVHWPYAGQKEHE--------VRYKVFKSLWQKGYFLT-SGSKFGGDFLVYPGDPMRFHAHFIAICFPYKEEIPLSDIVTAGRLGTNVKKTVLLCSADEGG--------------EVTFTSLQWSG 306
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. Ensembl Mouse
Match: Tsen34 (tRNA splicing endonuclease subunit 34 [Source:MGI Symbol;Acc:MGI:1913328]) HSP 1 Score: 62.003 bits (149), Expect = 9.982e-10 Identity = 71/301 (23.59%), Postives = 128/301 (42.52%), Query Frame = 3 Query: 1224 VWDPDSVRFLRDECRIFGKFIGSVARLPHKTVFKGLPLMLAVEEFVVLLKEHSERVRLVRSISLVSPLIPE--SSSASVDQYKSDIEHNYNEISDIIQEMRLDR----------------SNLHFDSVGD--SQIQGRKRKADGARVSIAPSVAPDLEKVSVHLPTSTPTDWLRVGEKETIDIPKLLSLAEVTDFVISLLQRKFEQKLPLDLVVKT---------------AVFYNLWSRGHCVTVSCKKMGGDYLLYPGDPWFHHATHVVRILPQTLEIKPSQLISKIRLINSLKKTYLI 2021 VW ++V+ LR+ + G+ +G++ R P + GLPL+L EE +L + + +++LVS P+ + ++ +K E ++ + + + E R R L DS D Q G G+ S + + E + L +S+P G +P+ L ++ ++ K PLD V++ +++ +LW RG ++ + K GGD+L+YPGDP HA ++ + I L+S RL S++KT L+ Sbjct: 13 VWGAEAVQALRERLGVGGRTVGALPRGPRQNSRLGLPLLLLPEEARLLAE--------IGAVTLVSAPRPDPRNHGLALASFKRQQEQSFQDQNTLAAEARETRRQELLEKIVEGQAAKKQKLEQDSGADEGGQEAGGSEATQGSETSDDGQPSAEQEGAAPSLDSSSPQPGPSNG---VTPLPRSALLIQLATARPRPVKAK-----PLDWRVQSKDWPHAGRPAHELRYSIYRDLWERGFFLSAAGK-FGGDFLVYPGDPLRFHAHYIAQCWSAEDPIPLQDLVSAGRLGTSVRKTLLL 296
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. Ensembl Mouse
Match: Tsen34 (tRNA splicing endonuclease subunit 34 [Source:MGI Symbol;Acc:MGI:1913328]) HSP 1 Score: 62.003 bits (149), Expect = 9.982e-10 Identity = 71/301 (23.59%), Postives = 128/301 (42.52%), Query Frame = 3 Query: 1224 VWDPDSVRFLRDECRIFGKFIGSVARLPHKTVFKGLPLMLAVEEFVVLLKEHSERVRLVRSISLVSPLIPE--SSSASVDQYKSDIEHNYNEISDIIQEMRLDR----------------SNLHFDSVGD--SQIQGRKRKADGARVSIAPSVAPDLEKVSVHLPTSTPTDWLRVGEKETIDIPKLLSLAEVTDFVISLLQRKFEQKLPLDLVVKT---------------AVFYNLWSRGHCVTVSCKKMGGDYLLYPGDPWFHHATHVVRILPQTLEIKPSQLISKIRLINSLKKTYLI 2021 VW ++V+ LR+ + G+ +G++ R P + GLPL+L EE +L + + +++LVS P+ + ++ +K E ++ + + + E R R L DS D Q G G+ S + + E + L +S+P G +P+ L ++ ++ K PLD V++ +++ +LW RG ++ + K GGD+L+YPGDP HA ++ + I L+S RL S++KT L+ Sbjct: 13 VWGAEAVQALRERLGVGGRTVGALPRGPRQNSRLGLPLLLLPEEARLLAE--------IGAVTLVSAPRPDPRNHGLALASFKRQQEQSFQDQNTLAAEARETRRQELLEKIVEGQAAKKQKLEQDSGADEGGQEAGGSEATQGSETSDDGQPSAEQEGAAPSLDSSSPQPGPSNG---VTPLPRSALLIQLATARPRPVKAK-----PLDWRVQSKDWPHAGRPAHELRYSIYRDLWERGFFLSAAGK-FGGDFLVYPGDPLRFHAHYIAQCWSAEDPIPLQDLVSAGRLGTSVRKTLLL 296
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. Ensembl Mouse
Match: Tsen34 (tRNA splicing endonuclease subunit 34 [Source:MGI Symbol;Acc:MGI:1913328]) HSP 1 Score: 60.8474 bits (146), Expect = 2.781e-9 Identity = 69/301 (22.92%), Postives = 126/301 (41.86%), Query Frame = 3 Query: 1224 VWDPDSVRFLRDECRIFGKFIGSVARLPHKTVFKGLPLMLAVEEFVVLLKEHSERVRLVRSISLVSPLIPE--SSSASVDQYKSDIEHNYNEISDIIQEMRLDR----------------SNLHFDSVGD--SQIQGRKRKADGARVSIAPSVAPDLEKVSVHLPTSTPTDWLRVGEKETIDIPKLLSLAEVTDFVISLLQRKFEQKLPLDLVVKT---------------AVFYNLWSRGHCVTVSCKKMGGDYLLYPGDPWFHHATHVVRILPQTLEIKPSQLISKIRLINSLKKTYLI 2021 VW ++V+ LR+ + G+ +G++ R P + GLPL+L EE +L + + +++LVS P+ + ++ +K E ++ + + + E R R L DS D Q G G+ S + + E P P++ + +P+ L ++ ++ K PLD V++ +++ +LW RG ++ + K GGD+L+YPGDP HA ++ + I L+S RL S++KT L+ Sbjct: 13 VWGAEAVQALRERLGVGGRTVGALPRGPRQNSRLGLPLLLLPEEARLLAE--------IGAVTLVSAPRPDPRNHGLALASFKRQQEQSFQDQNTLAAEARETRRQELLEKIVEGQAAKKQKLEQDSGADEGGQEAGGSEATQGSETSDDGQPSAEQEGADSSSPQPGPSNGV-------TPLPRSALLIQLATARPRPVKAK-----PLDWRVQSKDWPHAGRPAHELRYSIYRDLWERGFFLSAAGK-FGGDFLVYPGDPLRFHAHYIAQCWSAEDPIPLQDLVSAGRLGTSVRKTLLL 292
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. TrEMBL
Match: A0A3P7L0I8 (tRNA_int_endo domain-containing protein OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS6015 PE=4 SV=1) HSP 1 Score: 108.227 bits (269), Expect = 1.474e-22 Identity = 60/174 (34.48%), Postives = 95/174 (54.60%), Query Frame = 3 Query: 1641 VSVHLPTSTPTDWLRVGEKETIDIPKLL---SLAEVTDFVISLLQRKFEQKLPL-----------DLVVKTAVFYNLWSRGHCVTVSCKKMGGDYLLYPGDPWFHHATHVVRILPQTLEIKPSQLISKIRLINSLKKTYLIAWYDDNAVAKSSNLSSIVEHLNISFNCLGWCGS 2120 V +H P +TP +W R GE + +++P L L + ++I+L + + + ++K VF +LWS+G CVT S KMGGD+LLYPGDP F+HA+H+V + P T ++K +QL + +R+ NS++K L+A +AV + I + L W GS Sbjct: 63 VPIHRPRATPREWRRPGEHQQLEVPADLHADGLPAIVRWLITLSAKARASAACMPDSAVPKVDEEETILKCRVFADLWSKGFCVTGSAMKMGGDFLLYPGDPLFYHASHIVCVAPPTSQLKLAQLSTSLRIANSVRKVLLLA---TSAVKEGEE--------PIMYTSLYWTGS 225
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. TrEMBL
Match: A0A402FD12 (tRNA-splicing endonuclease subunit Sen34 OS=Paroedura picta OX=143630 GN=parPi_0016452 PE=3 SV=1) HSP 1 Score: 103.605 bits (257), Expect = 2.875e-20 Identity = 82/307 (26.71%), Postives = 139/307 (45.28%), Query Frame = 3 Query: 1224 VWDPDSVRFLRDECRIFGKFIGSVARLPHKTVFKGLPLMLAVEEFVVLLKEHSERVRLVRSISLVSPLIPESSS-----ASVDQYKSDIEHNYNEISDIIQEMRLDRSNLHFDSVGDSQIQGRKRKADGARVSIAPSVAPDLEKVSVH----LPTSTPTDWLRVGEKETIDIPKLLSLAEVTDFVISLLQRKFEQKLPLDLVVKTAVFYNLWSRGHCVTVSCKKMGGDYLLYPGDPWFHHATHVVRILPQTLEIKPSQLISKIRLINSLKKTYLIAWYDDNAVAKSSNLSSIVEHLNISFNCLGWCG 2117 VW+ D + +R+E R+ G +G++AR P + V GLPL L EE +L ++ L+++++ PL E+ +V Y+ D E +Y E + E R + + + + R+RK A + P+ DLE V + PT+ + G++E +D L +++ + ++ VF NLW +G+ +T S K GGD+L+YPGDP HA + +P+ + +IS RL S+KKT L+ D + N+++ L W G Sbjct: 41 VWNADDAQRIREEHRVVGTLVGALARKPRQNVRLGLPLQLLPEEARLLAEKGV--ATLLKNLT-SHPLTEEAGQDPSFLEAVAAYQKDQEESYQEQVRLAGEQRKALMGTLAERIAQGRAKKRQRKGGEAEPASKPA---DLESSFVFPREAMMVQLPTERTQRGQEEEVDW--------------RLPSKEWPYGRQPEHEMRFRVFQNLWEQGYYIT-SGSKFGGDFLVYPGDPMRFHAHFIALCVPRDTPLSLCDIISVGRLGTSVKKTVLLCSVDQDG--------------NVAYTSLQWSG 312
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. TrEMBL
Match: A0A183TSN8 (tRNA_int_endo domain-containing protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS19486 PE=4 SV=1) HSP 1 Score: 103.605 bits (257), Expect = 3.355e-20 Identity = 58/158 (36.71%), Postives = 87/158 (55.06%), Query Frame = 3 Query: 1605 SIAPSVAPDLEK----VSVHLPTSTPTDWLRVGEKETIDIPKLL---SLAEVTDFVISLLQRK-----------FEQKLPLDLVVKTAVFYNLWSRGHCVTVSCKKMGGDYLLYPGDPWFHHATHVVRILPQTLEIKPSQLISKIRLINSLKKTYLIA 2024 S A P E+ V +H P TP +W R GE + +D+P L L + ++I+L + Q ++K VF +LWS+G CVT S KMGG +LLYPGDP F+HA+H+V + P T ++K +QL + +R+ NS++K L+A Sbjct: 108 SYAGGTQPSTEQLHASVPIHRPRPTPREWRRHGEHQQLDVPAHLHTEGLPAIVRWLITLSSKARTSAANSSDSVVPQVDEEKTLLKCRVFMDLWSKGFCVTGSAMKMGGHFLLYPGDPLFYHASHIVCVAPATSQLKLAQLSTSLRIANSVRKVLLLA 265
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. TrEMBL
Match: H9GMZ3 (tRNA-splicing endonuclease subunit Sen34 OS=Anolis carolinensis OX=28377 GN=TSEN34 PE=3 SV=1) HSP 1 Score: 97.0561 bits (240), Expect = 4.393e-18 Identity = 88/303 (29.04%), Postives = 137/303 (45.21%), Query Frame = 3 Query: 1224 VWDPDSVRFLRDECRIFGKFIGSVARLPHKTVFKGLPLMLAVEEFVVLLKEHSERVRLVRSISLVSPLIPESSSASVDQYKSDIEHNYNEISDIIQEMRLDRSNLHFDSVGDSQIQGRKRKADGARVSIA-----PSVAPDLEKVSVHLPT-------STPTDWLRVGEKE-TIDIPKLLSLAEVTDFVISLLQ------RKFEQKLPL----DLVVKTAVFYNLWSRGHCVTVSCKKMGGDYLLYPGDPWFHHATHVVRILPQTLEIKPSQLISKIRLINSLKKTYLIAWYDDNAVAKSSNL 2063 VW+ VR +R+E RI G G++AR P + V GLPL L EE +LL E + + SP + E +S V Y+ ++E NY E + E R D++ + QGR +K PS L + S +PT S P ++ E E + +P+ + ++ I+ Q R + P D +F NLW RG VT S K GG++L+YPGDP HA ++ +P+ + +IS RL +++KKT L+ D++ ++L Sbjct: 13 VWNYADVRRIREEHRIVGTLFGALARKPRQNVRLGLPLQLLPEEARLLL----ENGLAILCKNPASPALGEDTSQEVAVYQKELEENYQEQCRLAAEQR----KALLDTLAERIAQGRAKKRQREGEEEGEQHCYPS---QLWRGSWMMPTGSDPPLLSFPESSSKLAEPEPSFVLPRDSMMVQLPTERINPGQEVVVDWRVPSKDWPYAGQPDHEAHYRIFRNLWERGFYVT-SGSKFGGNFLVYPGDPMRFHAHYIALCVPRDTPLSLCDIISAGRLGSNVKKTVLLCSADEDGTVVYTSL 303
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. TrEMBL
Match: A0A151M4W1 (tRNA-splicing endonuclease subunit Sen34 OS=Alligator mississippiensis OX=8496 GN=TSEN34 PE=3 SV=1) HSP 1 Score: 96.2857 bits (238), Expect = 4.825e-18 Identity = 83/299 (27.76%), Postives = 129/299 (43.14%), Query Frame = 3 Query: 1209 AAEIAVWDPDSVRFLRDECRIFGKFIGSVARLPHKTVFKGLPLMLAVEEFVVLLKEHSERVRLVRSISLVSPLIPESSSASVDQ---YKSDIEHNYNEISDIIQEMRLDRSNLHFDSVGDSQIQGRKRKAD--GARVSIAPSVAPDLEKVSVH---------LPTSTPTDWLRVGEKETIDIPKLLSLAEVTDFVISLLQRKFEQKLPLDLVVKTAVFYNLWSRGHCVTVSCKKMGGDYLLYPGDPWFHHATHVVRILPQTLEIKPSQLISKIRLINSLKKTYLIAWYDDNAVAKSSNL 2063 A + VW + R +R+ R+ G +G++AR P + V GLPL L EE LL E V LVRS P+ SA + Y+ +E +Y E + + R + ++ + QGR RK G AP P E+ + + PT W G++E +D + R + + + VF +LW RG+ VT S K GGD+L+YPGDP HA ++ P + +++ RL S+KKT L+ ++ V ++L Sbjct: 8 AGQALVWRFEDAREIRERHRLVGTLVGALARTPRQNVRLGLPLQLLPEE-ARLLAEMGAAV-LVRSADAQ----PQGQSAQASEGQLYEQGLEESYQEQRRLAAQERWAQ----LANLAECIAQGRARKQQQRGKLPDTAPLPGPTEEQEHLESAFVFPHESMMVQLPTVWPCPGQEEQVDW--------------TQPSRDWPHAGHQEHETRYCVFRDLWGRGYYVT-SGSKFGGDFLVYPGDPMRFHAHYIALCCPPGAPLPLCDIVAAARLGTSVKKTVLLCSAGEDGVVIYTSL 281
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. Ensembl Nematostella
Match: EDO32072 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SW35]) HSP 1 Score: 58.9214 bits (141), Expect = 1.095e-10 Identity = 34/92 (36.96%), Postives = 50/92 (54.35%), Query Frame = 3 Query: 1803 VKTAVFYNLWSRGHCVTVSCKKMGGDYLLYPGDPWFHHATHVVRILPQTLEIKPSQLISKIRLINSLKKTYLIAWYDD--NAVAKSSNLSSI 2072 ++ ++F NLW RG +T CK GGD+L YPGDP H+ ++V ++P ++S RL ++KKT + A D N V S S I Sbjct: 11 LRYSIFKNLWERGLYITSGCK-FGGDFLAYPGDPVKFHSFYIVVVVPYDKRTGMLDVVSMGRLGATVKKTAVFASMDSGGNVVYMSVKWSGI 101
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. Planmine SMEST
Match: SMESG000049881.1 (SMESG000049881.1) HSP 1 Score: 637.876 bits (1644), Expect = 0.000e+0 Identity = 312/314 (99.36%), Postives = 313/314 (99.68%), Query Frame = 3 Query: 1185 MVEITINSAAEIAVWDPDSVRFLRDECRIFGKFIGSVARLPHKTVFKGLPLMLAVEEFVVLLKEHSERVRLVRSISLVSPLIPESSSASVDQYKSDIEHNYNEISDIIQEMRLDRSNLHFDSVGDSQIQGRKRKADGARVSIAPSVAPDLEKVSVHLPTSTPTDWLRVGEKETIDIPKLLSLAEVTDFVISLLQRKFEQKLPLDLVVKTAVFYNLWSRGHCVTVSCKKMGGDYLLYPGDPWFHHATHVVRILPQTLEIKPSQLISKIRLINSLKKTYLIAWYDDNAVAKSSNLSSIVEHLNISFNCLGWCGSVP 2126 MVEITIN+AAEIAVWDPDSVRFLRDECRIFGKFIGSVARLPHKTVFKGLPLMLAVEEFVVLLKEHSERVRLVRSISLVSPLIPESSSASVDQYKSDIEHNYNEISDIIQEMRLDRSNLHFDSVGDSQIQGRKRKADGARVSIAPSVAPDLEKVSVHLPTSTPTDWLRVGEKETIDIPKLLSLAEVTDFVISLLQRKFEQKLPLD VVKTAVFYNLWSRGHCVTVSCKKMGGDYLLYPGDPWFHHATHVVRILPQTLEIKPSQLISKIRLINSLKKTYLIAWYDDNAVAKSSNLSSIVEHLNISFNCLGWCGSVP Sbjct: 1 MVEITINNAAEIAVWDPDSVRFLRDECRIFGKFIGSVARLPHKTVFKGLPLMLAVEEFVVLLKEHSERVRLVRSISLVSPLIPESSSASVDQYKSDIEHNYNEISDIIQEMRLDRSNLHFDSVGDSQIQGRKRKADGARVSIAPSVAPDLEKVSVHLPTSTPTDWLRVGEKETIDIPKLLSLAEVTDFVISLLQRKFEQKLPLDHVVKTAVFYNLWSRGHCVTVSCKKMGGDYLLYPGDPWFHHATHVVRILPQTLEIKPSQLISKIRLINSLKKTYLIAWYDDNAVAKSSNLSSIVEHLNISFNCLGWCGSVP 314
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. Planmine SMEST
Match: SMESG000049882.1 (SMESG000049882.1) HSP 1 Score: 255.373 bits (651), Expect = 9.014e-79 Identity = 121/122 (99.18%), Postives = 122/122 (100.00%), Query Frame = 2 Query: 671 ACPMFPERALDLTSTFRPKVFINPCSALSFGPSQMATGDSDSFQCQYPINLTIHHHSNGYYAYDSPVPIDLSYQTFWNSNHSSQPINFTNLLNSTSCPLDLTLDQSYSHEYDSNSTEIGWIC 1036 ACPMFPERALDLTSTFRPKVFINPCSALSFGPSQMATGDSDSFQCQYPINLTIHHHSNGYYAYDSPVPIDLSYQTFWNSNHSSQPINFTNLLNSTSCPLDLTLDQSYSHEYDSNS+EIGWIC Sbjct: 105 ACPMFPERALDLTSTFRPKVFINPCSALSFGPSQMATGDSDSFQCQYPINLTIHHHSNGYYAYDSPVPIDLSYQTFWNSNHSSQPINFTNLLNSTSCPLDLTLDQSYSHEYDSNSSEIGWIC 226 HSP 2 Score: 174.866 bits (442), Expect = 1.777e-49 Identity = 103/105 (98.10%), Postives = 103/105 (98.10%), Query Frame = 1 Query: 163 MRRHNNKNLVFQCPCCPRVYRDPSSLRRHKRLYEHKKNXXXXXXXXXXXXXXXXMNLSCEIPQSRKIITTQEEVIIKTTLPSIDTFAFNHSAIDCRINNSKDNQV 477 MRRHNNKNLVFQCPCCPRVYRDPSSLRRHKRLYEHKKNIEQEPEIPTPETVVIPMNLSCEI QSRKIITTQEEVIIKTTLPSIDTFAFNHSAIDCRINNSKDNQ Sbjct: 1 MRRHNNKNLVFQCPCCPRVYRDPSSLRRHKRLYEHKKNIEQEPEIPTPETVVIPMNLSCEITQSRKIITTQEEVIIKTTLPSIDTFAFNHSAIDCRINNSKDNQA 105
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. Planmine SMEST
Match: SMESG000049882.1 (SMESG000049882.1) HSP 1 Score: 255.758 bits (652), Expect = 1.398e-78 Identity = 121/122 (99.18%), Postives = 122/122 (100.00%), Query Frame = 2 Query: 671 ACPMFPERALDLTSTFRPKVFINPCSALSFGPSQMATGDSDSFQCQYPINLTIHHHSNGYYAYDSPVPIDLSYQTFWNSNHSSQPINFTNLLNSTSCPLDLTLDQSYSHEYDSNSTEIGWIC 1036 ACPMFPERALDLTSTFRPKVFINPCSALSFGPSQMATGDSDSFQCQYPINLTIHHHSNGYYAYDSPVPIDLSYQTFWNSNHSSQPINFTNLLNSTSCPLDLTLDQSYSHEYDSNS+EIGWIC Sbjct: 127 ACPMFPERALDLTSTFRPKVFINPCSALSFGPSQMATGDSDSFQCQYPINLTIHHHSNGYYAYDSPVPIDLSYQTFWNSNHSSQPINFTNLLNSTSCPLDLTLDQSYSHEYDSNSSEIGWIC 248 HSP 2 Score: 218.779 bits (556), Expect = 6.727e-65 Identity = 125/127 (98.43%), Postives = 125/127 (98.43%), Query Frame = 1 Query: 97 MSKHKCPECNKCFQNRSRKSLCMRRHNNKNLVFQCPCCPRVYRDPSSLRRHKRLYEHKKNXXXXXXXXXXXXXXXXMNLSCEIPQSRKIITTQEEVIIKTTLPSIDTFAFNHSAIDCRINNSKDNQV 477 MSKHKCPECNKCFQNRSRKSLCMRRHNNKNLVFQCPCCPRVYRDPSSLRRHKRLYEHKKNIEQEPEIPTPETVVIPMNLSCEI QSRKIITTQEEVIIKTTLPSIDTFAFNHSAIDCRINNSKDNQ Sbjct: 1 MSKHKCPECNKCFQNRSRKSLCMRRHNNKNLVFQCPCCPRVYRDPSSLRRHKRLYEHKKNIEQEPEIPTPETVVIPMNLSCEITQSRKIITTQEEVIIKTTLPSIDTFAFNHSAIDCRINNSKDNQA 127 The following BLAST results are available for this feature:
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 1
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 2
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 1
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 3
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of tRNA-splicing endonuclease subunit Sen34 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30025404 ID=SMED30025404|Name=tRNA-splicing endonuclease subunit Sen34|organism=Schmidtea mediterranea sexual|type=transcript|length=2720bpback to top protein sequence of SMED30025404-orf-1 >SMED30025404-orf-1 ID=SMED30025404-orf-1|Name=SMED30025404-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=144bp MSKHKCPECNKCFQNRSRKSLCMRRHNNKNLVFQCPCCPRVYRDPSSLRRback to top protein sequence of SMED30025404-orf-2 >SMED30025404-orf-2 ID=SMED30025404-orf-2|Name=SMED30025404-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=327bp MNLVCEIGRFQQMVEITINSAAEIAVWDPDSVRFLRDECRIFGKFIGSVAback to top protein sequence of SMED30025404-orf-3 >SMED30025404-orf-3 ID=SMED30025404-orf-3|Name=SMED30025404-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=120bp MFPERALDLTSTFRPKVFINPCSALSFGPSQMATGDSDSFQCQYPINLTIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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