SMED30025363

Overview
NameSMED30025363
Smed IDSMED30025363
Length (bp)2376
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30025363 (SMED30025363) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30025363 (SMED30025363) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30025363 (SMED30025363) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30025363 (SMED30025363) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30025363

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 19

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30025363h1SMcG0019657 Contig45929uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025363h1SMcG0019657 Contig45929newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30025363h1SMcG0019657 dd_Smed_v4_1473_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30025363h1SMcG0019657 dd_Smed_v4_1473_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30025363h1SMcG0019657 dd_Smed_v4_1473_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30025363h1SMcG0019657 dd_Smed_v4_1473_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30025363h1SMcG0019657 dd_Smed_v4_1473_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025363h1SMcG0019657 Contig37481newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025363h1SMcG0019657 Contig37481uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30025363h1SMcG0019657 dd_Smed_v4_1754_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30025363h1SMcG0019657 dd_Smed_v4_1754_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30025363h1SMcG0019657 dd_Smed_v4_1754_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30025363h1SMcG0019657 dd_Smed_v4_1754_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
tail regionSMED30025363h1SMcG0019657 dd_Smed_v6_1754_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
gutSMED30025363h1SMcG0019657 SMED30010528smed_20140614PMID:26457503
Tu et al., 2015		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30025363h1SMcG0019657 SMED30010528smed_20140614PMID:26457503
Tu et al., 2015		whole organism asexual adult colorimetric in situ hybridization evidence
body wall musculatureSMED30025363h1SMcG0019657 dd_Smed_v4_1754_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30025363h1SMcG0019657 dd_Smed_v6_1473_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 2 cellSMED30025363h1SMcG0019657 dd_Smed_v4_1754_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30025363 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002992:3110..6557 +10704
Homology
[Close]
BLAST of SMED30025363 vs. Planmine SMEST
Match: SMESG000014711.1 (SMESG000014711.1)

HSP 1 Score: 1278.08 bits (3306), Expect = 0.000e+0
Identity = 649/663 (97.89%), Postives = 659/663 (99.40%), Query Frame = 3 
Query:  156 MSFFLKMMLKNFILTLGGFLVLPLVTLANQMFIMKVESTTKFPFYFNTPFKIKFVPDSLNGTISVNSDFASLEIRITDKTEFEFERLQILYKSESKVYSGDFFTILSWRRNVIENYIELILLFPMAASNKLVEIYEISAANEAEYFEIKENSKLARXXXXXXXXXXXXXXXXXXFTPFHGKRDEFLSGETVTVTCQSSGFAILTRPNISNLNEFTVVSELNSEIVNEIKVKIKLQSSDHGKTLKCDAGKLTGKFSINVMYAPSNVKISPDFNSVVKINSNISCDPGNGYPIPNTSWTLVKGPSNLPVLYSIDKEKRKIKFIRSPLSDSEWIFNCSARFKLNNSDGIYHKSVKFMSEGDKITILDIDNIETMIIIKVSEMKRYIDSHMNHMANNLNFVLKENAKMNQTNKELLNEISMLKNIMKNYSVNAKELKENIKYEIGKMNITYKKLVSKSEISTNLDSVVPKMEFQKLKEDVIKQNDDISGLKLKTEFIEMDLKTEKTLSKIVQQAIKAMIQSNEALKRQIAFTKFHLFWIVPITWTVTGIDVDESVRKKEATNDPFYTQIKNYKLHLYASFTPTKIEYYLYLYDNRRNKRNGIPFKSQITISVVHKLLPNVKLDRTIYSKIYTIPGVSKTISFTRTELNKLGFLEDNEITIICDVKEM 2144
            M+FFLKMMLKNFILTLGGFLVLPLVTLANQMFIMKVESTTKFPFYFNTPFKIKFVPDSLNGTISVNSDFASLEIRITDKTEFEFERLQILYKSESKVYSGDFFTILSWRRNVI+NYIELILLFPMAASNKLVEIYEISAANEAEYFEIKENSKLARLLYKSKIILSLSPKV+LSFTPFHGKRDEFLSGETVTVTCQSSGFAILTRPNISNLNEFTVVSELNSE+VNEIKVKIKLQSSDHGKTLKCDAGKLTGKFSINV+YAPSNVKISPDFNSVVKINSNISCDPGNGYPIPNTSWTLVKGPSNLPVLYSIDKEKRKIKFIRSPLSDSEWIFNCSAR+KLNNSDGIYHKSVKFMSEGDKITILDIDNIETMIIIKVSEMKRYIDSHMN MANNLNF+LKENAKMNQTNKELLNEISMLKNIMKNYSVNAKELKENIKYEIGKMNITYKKLVSKSEISTNLDS VPKMEFQKLKEDVIKQNDDISGLKLKTEFIEMDLKTEKTLSKIVQQAIKAMI+SNEALK QIAFTKFHLFWIVPITWTVTGIDVDESV+KKEATNDPFYTQIKNYKLHLYASFTPTKIEYYLYLYDNRRNKRNGIPFKSQITISVVHKLLPNVKLDRTIYSKIYTIPGVSKTISFTRTELNKLGFLE+N ITIICDVKEM
Sbjct:    1 MTFFLKMMLKNFILTLGGFLVLPLVTLANQMFIMKVESTTKFPFYFNTPFKIKFVPDSLNGTISVNSDFASLEIRITDKTEFEFERLQILYKSESKVYSGDFFTILSWRRNVIKNYIELILLFPMAASNKLVEIYEISAANEAEYFEIKENSKLARLLYKSKIILSLSPKVELSFTPFHGKRDEFLSGETVTVTCQSSGFAILTRPNISNLNEFTVVSELNSEMVNEIKVKIKLQSSDHGKTLKCDAGKLTGKFSINVIYAPSNVKISPDFNSVVKINSNISCDPGNGYPIPNTSWTLVKGPSNLPVLYSIDKEKRKIKFIRSPLSDSEWIFNCSARYKLNNSDGIYHKSVKFMSEGDKITILDIDNIETMIIIKVSEMKRYIDSHMNRMANNLNFMLKENAKMNQTNKELLNEISMLKNIMKNYSVNAKELKENIKYEIGKMNITYKKLVSKSEISTNLDSGVPKMEFQKLKEDVIKQNDDISGLKLKTEFIEMDLKTEKTLSKIVQQAIKAMIKSNEALKSQIAFTKFHLFWIVPITWTVTGIDVDESVKKKEATNDPFYTQIKNYKLHLYASFTPTKIEYYLYLYDNRRNKRNGIPFKSQITISVVHKLLPNVKLDRTIYSKIYTIPGVSKTISFTRTELNKLGFLEENRITIICDVKEM 663
[Close]
BLAST of SMED30025363 vs. Planmine SMEST
Match: SMESG000014711.1 (SMESG000014711.1)

HSP 1 Score: 1267.68 bits (3279), Expect = 0.000e+0
Identity = 644/657 (98.02%), Postives = 653/657 (99.39%), Query Frame = 3 
Query:  174 MMLKNFILTLGGFLVLPLVTLANQMFIMKVESTTKFPFYFNTPFKIKFVPDSLNGTISVNSDFASLEIRITDKTEFEFERLQILYKSESKVYSGDFFTILSWRRNVIENYIELILLFPMAASNKLVEIYEISAANEAEYFEIKENSKLARXXXXXXXXXXXXXXXXXXFTPFHGKRDEFLSGETVTVTCQSSGFAILTRPNISNLNEFTVVSELNSEIVNEIKVKIKLQSSDHGKTLKCDAGKLTGKFSINVMYAPSNVKISPDFNSVVKINSNISCDPGNGYPIPNTSWTLVKGPSNLPVLYSIDKEKRKIKFIRSPLSDSEWIFNCSARFKLNNSDGIYHKSVKFMSEGDKITILDIDNIETMIIIKVSEMKRYIDSHMNHMANNLNFVLKENAKMNQTNKELLNEISMLKNIMKNYSVNAKELKENIKYEIGKMNITYKKLVSKSEISTNLDSVVPKMEFQKLKEDVIKQNDDISGLKLKTEFIEMDLKTEKTLSKIVQQAIKAMIQSNEALKRQIAFTKFHLFWIVPITWTVTGIDVDESVRKKEATNDPFYTQIKNYKLHLYASFTPTKIEYYLYLYDNRRNKRNGIPFKSQITISVVHKLLPNVKLDRTIYSKIYTIPGVSKTISFTRTELNKLGFLEDNEITIICDVKEM 2144
            MMLKNFILTLGGFLVLPLVTLANQMFIMKVESTTKFPFYFNTPFKIKFVPDSLNGTISVNSDFASLEIRITDKTEFEFERLQILYKSESKVYSGDFFTILSWRRNVI+NYIELILLFPMAASNKLVEIYEISAANEAEYFEIKENSKLARLLYKSKIILSLSPKV+LSFTPFHGKRDEFLSGETVTVTCQSSGFAILTRPNISNLNEFTVVSELNSE+VNEIKVKIKLQSSDHGKTLKCDAGKLTGKFSINV+YAPSNVKISPDFNSVVKINSNISCDPGNGYPIPNTSWTLVKGPSNLPVLYSIDKEKRKIKFIRSPLSDSEWIFNCSAR+KLNNSDGIYHKSVKFMSEGDKITILDIDNIETMIIIKVSEMKRYIDSHMN MANNLNF+LKENAKMNQTNKELLNEISMLKNIMKNYSVNAKELKENIKYEIGKMNITYKKLVSKSEISTNLDS VPKMEFQKLKEDVIKQNDDISGLKLKTEFIEMDLKTEKTLSKIVQQAIKAMI+SNEALK QIAFTKFHLFWIVPITWTVTGIDVDESV+KKEATNDPFYTQIKNYKLHLYASFTPTKIEYYLYLYDNRRNKRNGIPFKSQITISVVHKLLPNVKLDRTIYSKIYTIPGVSKTISFTRTELNKLGFLE+N ITIICDVKEM
Sbjct:    1 MMLKNFILTLGGFLVLPLVTLANQMFIMKVESTTKFPFYFNTPFKIKFVPDSLNGTISVNSDFASLEIRITDKTEFEFERLQILYKSESKVYSGDFFTILSWRRNVIKNYIELILLFPMAASNKLVEIYEISAANEAEYFEIKENSKLARLLYKSKIILSLSPKVELSFTPFHGKRDEFLSGETVTVTCQSSGFAILTRPNISNLNEFTVVSELNSEMVNEIKVKIKLQSSDHGKTLKCDAGKLTGKFSINVIYAPSNVKISPDFNSVVKINSNISCDPGNGYPIPNTSWTLVKGPSNLPVLYSIDKEKRKIKFIRSPLSDSEWIFNCSARYKLNNSDGIYHKSVKFMSEGDKITILDIDNIETMIIIKVSEMKRYIDSHMNRMANNLNFMLKENAKMNQTNKELLNEISMLKNIMKNYSVNAKELKENIKYEIGKMNITYKKLVSKSEISTNLDSGVPKMEFQKLKEDVIKQNDDISGLKLKTEFIEMDLKTEKTLSKIVQQAIKAMIKSNEALKSQIAFTKFHLFWIVPITWTVTGIDVDESVKKKEATNDPFYTQIKNYKLHLYASFTPTKIEYYLYLYDNRRNKRNGIPFKSQITISVVHKLLPNVKLDRTIYSKIYTIPGVSKTISFTRTELNKLGFLEENRITIICDVKEM 657
[Close]
BLAST of SMED30025363 vs. Planmine SMEST
Match: SMESG000014711.1 (SMESG000014711.1)

HSP 1 Score: 1218.76 bits (3152), Expect = 0.000e+0
Identity = 620/633 (97.95%), Postives = 629/633 (99.37%), Query Frame = 3 
Query:  246 MFIMKVESTTKFPFYFNTPFKIKFVPDSLNGTISVNSDFASLEIRITDKTEFEFERLQILYKSESKVYSGDFFTILSWRRNVIENYIELILLFPMAASNKLVEIYEISAANEAEYFEIKENSKLARXXXXXXXXXXXXXXXXXXFTPFHGKRDEFLSGETVTVTCQSSGFAILTRPNISNLNEFTVVSELNSEIVNEIKVKIKLQSSDHGKTLKCDAGKLTGKFSINVMYAPSNVKISPDFNSVVKINSNISCDPGNGYPIPNTSWTLVKGPSNLPVLYSIDKEKRKIKFIRSPLSDSEWIFNCSARFKLNNSDGIYHKSVKFMSEGDKITILDIDNIETMIIIKVSEMKRYIDSHMNHMANNLNFVLKENAKMNQTNKELLNEISMLKNIMKNYSVNAKELKENIKYEIGKMNITYKKLVSKSEISTNLDSVVPKMEFQKLKEDVIKQNDDISGLKLKTEFIEMDLKTEKTLSKIVQQAIKAMIQSNEALKRQIAFTKFHLFWIVPITWTVTGIDVDESVRKKEATNDPFYTQIKNYKLHLYASFTPTKIEYYLYLYDNRRNKRNGIPFKSQITISVVHKLLPNVKLDRTIYSKIYTIPGVSKTISFTRTELNKLGFLEDNEITIICDVKEM 2144
            MFIMKVESTTKFPFYFNTPFKIKFVPDSLNGTISVNSDFASLEIRITDKTEFEFERLQILYKSESKVYSGDFFTILSWRRNVI+NYIELILLFPMAASNKLVEIYEISAANEAEYFEIKENSKLARLLYKSKIILSLSPKV+LSFTPFHGKRDEFLSGETVTVTCQSSGFAILTRPNISNLNEFTVVSELNSE+VNEIKVKIKLQSSDHGKTLKCDAGKLTGKFSINV+YAPSNVKISPDFNSVVKINSNISCDPGNGYPIPNTSWTLVKGPSNLPVLYSIDKEKRKIKFIRSPLSDSEWIFNCSAR+KLNNSDGIYHKSVKFMSEGDKITILDIDNIETMIIIKVSEMKRYIDSHMN MANNLNF+LKENAKMNQTNKELLNEISMLKNIMKNYSVNAKELKENIKYEIGKMNITYKKLVSKSEISTNLDS VPKMEFQKLKEDVIKQNDDISGLKLKTEFIEMDLKTEKTLSKIVQQAIKAMI+SNEALK QIAFTKFHLFWIVPITWTVTGIDVDESV+KKEATNDPFYTQIKNYKLHLYASFTPTKIEYYLYLYDNRRNKRNGIPFKSQITISVVHKLLPNVKLDRTIYSKIYTIPGVSKTISFTRTELNKLGFLE+N ITIICDVKEM
Sbjct:    1 MFIMKVESTTKFPFYFNTPFKIKFVPDSLNGTISVNSDFASLEIRITDKTEFEFERLQILYKSESKVYSGDFFTILSWRRNVIKNYIELILLFPMAASNKLVEIYEISAANEAEYFEIKENSKLARLLYKSKIILSLSPKVELSFTPFHGKRDEFLSGETVTVTCQSSGFAILTRPNISNLNEFTVVSELNSEMVNEIKVKIKLQSSDHGKTLKCDAGKLTGKFSINVIYAPSNVKISPDFNSVVKINSNISCDPGNGYPIPNTSWTLVKGPSNLPVLYSIDKEKRKIKFIRSPLSDSEWIFNCSARYKLNNSDGIYHKSVKFMSEGDKITILDIDNIETMIIIKVSEMKRYIDSHMNRMANNLNFMLKENAKMNQTNKELLNEISMLKNIMKNYSVNAKELKENIKYEIGKMNITYKKLVSKSEISTNLDSGVPKMEFQKLKEDVIKQNDDISGLKLKTEFIEMDLKTEKTLSKIVQQAIKAMIKSNEALKSQIAFTKFHLFWIVPITWTVTGIDVDESVKKKEATNDPFYTQIKNYKLHLYASFTPTKIEYYLYLYDNRRNKRNGIPFKSQITISVVHKLLPNVKLDRTIYSKIYTIPGVSKTISFTRTELNKLGFLEENRITIICDVKEM 633
[Close]
BLAST of SMED30025363 vs. Planmine SMEST
Match: SMESG000014711.1 (SMESG000014711.1)

HSP 1 Score: 1213.75 bits (3139), Expect = 0.000e+0
Identity = 617/630 (97.94%), Postives = 626/630 (99.37%), Query Frame = 3 
Query:  255 MKVESTTKFPFYFNTPFKIKFVPDSLNGTISVNSDFASLEIRITDKTEFEFERLQILYKSESKVYSGDFFTILSWRRNVIENYIELILLFPMAASNKLVEIYEISAANEAEYFEIKENSKLARXXXXXXXXXXXXXXXXXXFTPFHGKRDEFLSGETVTVTCQSSGFAILTRPNISNLNEFTVVSELNSEIVNEIKVKIKLQSSDHGKTLKCDAGKLTGKFSINVMYAPSNVKISPDFNSVVKINSNISCDPGNGYPIPNTSWTLVKGPSNLPVLYSIDKEKRKIKFIRSPLSDSEWIFNCSARFKLNNSDGIYHKSVKFMSEGDKITILDIDNIETMIIIKVSEMKRYIDSHMNHMANNLNFVLKENAKMNQTNKELLNEISMLKNIMKNYSVNAKELKENIKYEIGKMNITYKKLVSKSEISTNLDSVVPKMEFQKLKEDVIKQNDDISGLKLKTEFIEMDLKTEKTLSKIVQQAIKAMIQSNEALKRQIAFTKFHLFWIVPITWTVTGIDVDESVRKKEATNDPFYTQIKNYKLHLYASFTPTKIEYYLYLYDNRRNKRNGIPFKSQITISVVHKLLPNVKLDRTIYSKIYTIPGVSKTISFTRTELNKLGFLEDNEITIICDVKEM 2144
            MKVESTTKFPFYFNTPFKIKFVPDSLNGTISVNSDFASLEIRITDKTEFEFERLQILYKSESKVYSGDFFTILSWRRNVI+NYIELILLFPMAASNKLVEIYEISAANEAEYFEIKENSKLARLLYKSKIILSLSPKV+LSFTPFHGKRDEFLSGETVTVTCQSSGFAILTRPNISNLNEFTVVSELNSE+VNEIKVKIKLQSSDHGKTLKCDAGKLTGKFSINV+YAPSNVKISPDFNSVVKINSNISCDPGNGYPIPNTSWTLVKGPSNLPVLYSIDKEKRKIKFIRSPLSDSEWIFNCSAR+KLNNSDGIYHKSVKFMSEGDKITILDIDNIETMIIIKVSEMKRYIDSHMN MANNLNF+LKENAKMNQTNKELLNEISMLKNIMKNYSVNAKELKENIKYEIGKMNITYKKLVSKSEISTNLDS VPKMEFQKLKEDVIKQNDDISGLKLKTEFIEMDLKTEKTLSKIVQQAIKAMI+SNEALK QIAFTKFHLFWIVPITWTVTGIDVDESV+KKEATNDPFYTQIKNYKLHLYASFTPTKIEYYLYLYDNRRNKRNGIPFKSQITISVVHKLLPNVKLDRTIYSKIYTIPGVSKTISFTRTELNKLGFLE+N ITIICDVKEM
Sbjct:    1 MKVESTTKFPFYFNTPFKIKFVPDSLNGTISVNSDFASLEIRITDKTEFEFERLQILYKSESKVYSGDFFTILSWRRNVIKNYIELILLFPMAASNKLVEIYEISAANEAEYFEIKENSKLARLLYKSKIILSLSPKVELSFTPFHGKRDEFLSGETVTVTCQSSGFAILTRPNISNLNEFTVVSELNSEMVNEIKVKIKLQSSDHGKTLKCDAGKLTGKFSINVIYAPSNVKISPDFNSVVKINSNISCDPGNGYPIPNTSWTLVKGPSNLPVLYSIDKEKRKIKFIRSPLSDSEWIFNCSARYKLNNSDGIYHKSVKFMSEGDKITILDIDNIETMIIIKVSEMKRYIDSHMNRMANNLNFMLKENAKMNQTNKELLNEISMLKNIMKNYSVNAKELKENIKYEIGKMNITYKKLVSKSEISTNLDSGVPKMEFQKLKEDVIKQNDDISGLKLKTEFIEMDLKTEKTLSKIVQQAIKAMIKSNEALKSQIAFTKFHLFWIVPITWTVTGIDVDESVKKKEATNDPFYTQIKNYKLHLYASFTPTKIEYYLYLYDNRRNKRNGIPFKSQITISVVHKLLPNVKLDRTIYSKIYTIPGVSKTISFTRTELNKLGFLEENRITIICDVKEM 630
[Close]
BLAST of SMED30025363 vs. Planmine SMEST
Match: SMESG000014705.1 (SMESG000014705.1)

HSP 1 Score: 922.154 bits (2382), Expect = 0.000e+0
Identity = 489/658 (74.32%), Postives = 549/658 (83.43%), Query Frame = 3 
Query:  174 MMLKNFILTLGGFLVLPLVTLANQMFIMKVESTTKFPFYFNTPFKIKFVPDSLNGTISVNSDFASLEIRITDKTEFEFERLQILYKSESKVYSGDFFTILSWRRNVIENYIELILLFPMAASNKLVEIYEISAANEAEYFEIKENSKLARXXXXXXXXXXXXXXXXXXFTPFHGKRDEFLSGETVTVTCQSSGFAILTRPNISNLNEFTVVSELNSEIVNEIKVKIKLQSSDHGKTLKCDAGKLTGKFSINVMYAPSNVKISPDFNSVVKINSNISCDPGNGYPIPNTSWTLVKGPSNLPVLYSIDKEKRKIKFIRSPLSDSEWIFNCSARFKLNNSDGIYHKSVKFMSEGDKITILDIDNIETMIIIKVSEMKRYIDSHMNHMANNLNFVLKENAKMNQTNKELLNEISMLKNIMKNYSVNAKELKENIKYEIGKMNITYKKLVSKSEISTNL-DSVVPKMEFQKLKEDVIKQNDDISGLKLKTEFIEMDLKTEKTLSKIVQQAIKAMIQSNEALKRQIAFTKFHLFWIVPITWTVTGIDVDESVRKKEATNDPFYTQIKNYKLHLYASFTPTKIEYYLYLYDNRRNKRNGIPFKSQITISVVHKLLPNVKLDRTIYSKIYTIPGVSKTISFTRTELNKLGFLEDNEITIICDVKEM 2144
            MMLKNFILTLGGFLVLPLVTLANQ FIMKVESTTKFPFYFNTPFKIKFVPDSLNGTISVNSDFASLEIRITDKTEFEFERLQILYKSESKVYSGDFFTILSWRRNVI+NYIELILLFPMAASNKLVEIYEISAANEAEYFEIKENSKLARLLYKSKIILSLSPKVKLSFT FHGKRDEFLSGETVTVTCQSSGFAILTRPNISNLNEFTVVSELNSE+VNEIKVKIKLQSSDHGKTLKCDAGKLTGKFSINVMYAPSNVKISPDFNSVVKINSNISCDPGNGYPIPNTSWTLVKGPSNLPVLYSIDKEKRKIKFIRS LSDSEWIFNCSAR+KLNNSDGIYHKSVKFMSEG++I ILD+ N+ET I IK+ + K+Y+ + ++ +         EN ++     +LLNEI MLK  M NYS      KE+ KYE              S+IS N+ D ++   EFQKLKEDVIKQNDDISGLKLKTE I+M+L  ++T+++ + + +KA   ++E     I F   HLFW+VPI+  V  ID+++    KE  ++ FYTQIKNY+L+L++ FTP  IE+Y+YL  +RRNK + +PFKSQITI+VVHKLLP++ LDR I S I T PG+S ++ FTR ELN+LG L  N I I CDV+E+
Sbjct:    1 MMLKNFILTLGGFLVLPLVTLANQRFIMKVESTTKFPFYFNTPFKIKFVPDSLNGTISVNSDFASLEIRITDKTEFEFERLQILYKSESKVYSGDFFTILSWRRNVIKNYIELILLFPMAASNKLVEIYEISAANEAEYFEIKENSKLARLLYKSKIILSLSPKVKLSFTSFHGKRDEFLSGETVTVTCQSSGFAILTRPNISNLNEFTVVSELNSEMVNEIKVKIKLQSSDHGKTLKCDAGKLTGKFSINVMYAPSNVKISPDFNSVVKINSNISCDPGNGYPIPNTSWTLVKGPSNLPVLYSIDKEKRKIKFIRSSLSDSEWIFNCSARYKLNNSDGIYHKSVKFMSEGEEIIILDMGNLETFIKIKLIQSKKYVSTELSKLKRQFAI---ENGRVKDIFNKLLNEIPMLKQKMNNYS------KEDAKYE-------------GSKISQNIQDLLIGTKEFQKLKEDVIKQNDDISGLKLKTETIKMNLNYDRTITQAIVKQLKASALTHEEFDTDIKFALTHLFWLVPISIRVRNIDIEDEGPNKEHVSNAFYTQIKNYELYLHSLFTPKNIEFYVYLSASRRNK-HSLPFKSQITIAVVHKLLPSINLDRKILSTIRTAPGISDSVKFTRVELNRLGLLYGNTIKINCDVQEI 635
The following BLAST results are available for this feature:
BLAST of SMED30025363 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025363 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025363 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025363 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025363 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025363 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025363 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025363 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025363 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025363 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025363 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025363 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025363 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025363 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000014711.10.000e+097.89SMESG000014711.1[more]
SMESG000014711.10.000e+098.02SMESG000014711.1[more]
SMESG000014711.10.000e+097.95SMESG000014711.1[more]
SMESG000014711.10.000e+097.94SMESG000014711.1[more]
SMESG000014705.10.000e+074.32SMESG000014705.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30025363 ID=SMED30025363|Name=SMED30025363|organism=Schmidtea mediterranea sexual|type=transcript|length=2376bp
TTGATTATCAAAACTTTTTAATTATCAAAAGCTGTTTACAATTTCAATTT
CAAAAAATTAAAATTTAAAATTTTAAAAAAAATTCATTATTTTGTTTTAC
TGAATAAATTCAAGTTGAATATAAATTAAACGTTTATCAGATTTATTTTC
ATTTAATGTCATTTTTTTTGAAAATGATGCTTAAAAATTTTATTTTAACA
TTGGGAGGATTCCTCGTGCTACCCCTTGTCACTCTGGCAAACCAAATGTT
CATCATGAAGGTCGAGTCAACCACAAAATTTCCATTCTATTTCAATACTC
CCTTCAAAATTAAATTCGTTCCCGATTCATTGAATGGAACAATTTCGGTG
AACTCAGATTTTGCTTCCTTGGAAATCCGAATTACAGACAAAACAGAGTT
CGAATTTGAGAGGCTTCAAATCCTTTACAAATCGGAGTCGAAAGTTTATT
CTGGAGACTTTTTCACGATTTTAAGCTGGAGAAGAAATGTCATCGAAAAC
TATATCGAGTTGATTTTGTTATTCCCGATGGCGGCAAGCAACAAATTAGT
AGAGATCTATGAAATATCCGCGGCGAATGAAGCCGAGTATTTCGAAATCA
AGGAAAATAGCAAACTAGCAAGGCTTTTGTACAAGTCAAAAATAATATTA
TCTTTATCCCCAAAAGTTAAGTTGTCATTCACGCCTTTTCATGGAAAACG
AGATGAATTCCTGTCAGGTGAAACTGTGACAGTAACATGCCAGTCCTCGG
GATTTGCCATTCTGACTCGGCCGAATATCTCAAATTTGAATGAGTTTACA
GTCGTATCCGAACTCAATTCAGAAATAGTAAATGAGATCAAGGTAAAGAT
AAAATTACAGAGCTCTGATCATGGGAAAACGCTGAAATGCGATGCAGGAA
AGCTAACTGGAAAATTCTCTATCAATGTTATGTATGCTCCTTCGAATGTC
AAGATCTCCCCAGATTTCAACAGTGTTGTTAAAATAAATTCCAACATTTC
ATGCGATCCAGGAAACGGCTATCCTATTCCCAACACATCCTGGACCCTTG
TAAAAGGTCCGAGTAATTTACCAGTACTATATTCCATCGACAAGGAAAAA
AGGAAGATTAAATTCATTAGAAGCCCTCTTTCAGATTCTGAATGGATTTT
CAATTGCAGCGCTCGCTTTAAGCTGAATAATTCGGACGGGATTTACCACA
AAAGTGTGAAATTCATGAGTGAAGGCGACAAGATTACGATCTTGGATATC
GACAACATTGAAACAATGATTATTATTAAAGTTTCTGAAATGAAAAGATA
TATCGACAGCCACATGAATCACATGGCAAATAATCTTAATTTCGTGCTGA
AAGAGAATGCCAAAATGAATCAAACAAACAAAGAACTATTGAACGAAATT
TCAATGCTTAAAAATATTATGAAAAATTATTCAGTGAATGCAAAAGAGCT
TAAAGAAAACATAAAATATGAGATTGGAAAAATGAATATAACATACAAAA
AGCTTGTGAGTAAATCCGAAATTTCAACAAATCTAGACTCAGTTGTACCC
AAAATGGAATTTCAAAAGTTAAAGGAAGATGTCATCAAGCAGAACGACGA
CATCAGTGGACTGAAATTGAAGACGGAATTCATTGAAATGGACTTGAAAA
CAGAAAAAACGCTTTCTAAAATAGTTCAGCAAGCAATTAAAGCAATGATT
CAATCTAATGAAGCTTTGAAGAGACAGATAGCGTTCACTAAATTTCACTT
GTTCTGGATTGTCCCAATCACATGGACTGTCACTGGCATTGACGTTGATG
AGTCTGTGAGGAAAAAAGAAGCCACAAACGACCCTTTTTATACCCAAATC
AAAAATTATAAACTCCATCTTTATGCATCCTTTACTCCTACGAAAATTGA
ATATTATTTGTACCTCTATGACAATCGACGAAACAAGCGAAATGGTATAC
CATTCAAAAGTCAAATTACTATCTCCGTCGTTCACAAATTGTTGCCGAAT
GTAAAATTAGACCGAACAATCTACTCAAAAATATACACGATCCCAGGAGT
GAGTAAGACGATCTCATTTACGAGAACGGAACTCAACAAACTCGGCTTCC
TCGAGGACAATGAAATAACAATAATTTGCGATGTTAAAGAAATGTGAGAA
ACAAATTTCCATAAACTTTAGGATAACTTTTTTTATAATAACAATGTTTA
TGTAATACTATTATTGTTCTAATTTTTAACAATTTAATTATATAAATTGT
CAATTTATTTTAATAACAGCGGATTTTTGAATAACATTGAAACAAAAGGT
CATCTTGTTGATTACAAGGAAAATATTTGAAAAAATTGTAATTAACAGTA
TTTATCTAACTTACGCATCTTTTAAA
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protein sequence of SMED30025363-orf-1

>SMED30025363-orf-1 ID=SMED30025363-orf-1|Name=SMED30025363-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=664bp
MSFFLKMMLKNFILTLGGFLVLPLVTLANQMFIMKVESTTKFPFYFNTPF
KIKFVPDSLNGTISVNSDFASLEIRITDKTEFEFERLQILYKSESKVYSG
DFFTILSWRRNVIENYIELILLFPMAASNKLVEIYEISAANEAEYFEIKE
NSKLARLLYKSKIILSLSPKVKLSFTPFHGKRDEFLSGETVTVTCQSSGF
AILTRPNISNLNEFTVVSELNSEIVNEIKVKIKLQSSDHGKTLKCDAGKL
TGKFSINVMYAPSNVKISPDFNSVVKINSNISCDPGNGYPIPNTSWTLVK
GPSNLPVLYSIDKEKRKIKFIRSPLSDSEWIFNCSARFKLNNSDGIYHKS
VKFMSEGDKITILDIDNIETMIIIKVSEMKRYIDSHMNHMANNLNFVLKE
NAKMNQTNKELLNEISMLKNIMKNYSVNAKELKENIKYEIGKMNITYKKL
VSKSEISTNLDSVVPKMEFQKLKEDVIKQNDDISGLKLKTEFIEMDLKTE
KTLSKIVQQAIKAMIQSNEALKRQIAFTKFHLFWIVPITWTVTGIDVDES
VRKKEATNDPFYTQIKNYKLHLYASFTPTKIEYYLYLYDNRRNKRNGIPF
KSQITISVVHKLLPNVKLDRTIYSKIYTIPGVSKTISFTRTELNKLGFLE
DNEITIICDVKEM*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000028Category 2 cell
PLANA:0000101muscle cell
PLANA:0000240body wall musculature
PLANA:0000421tail
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR013783Ig-like_fold
IPR002083MATH/TRAF_dom
IPR008974TRAF-like
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 394..435
NoneNo IPR availableTMHMMTMhelixcoord: 12..34
IPR013783Immunoglobulin-like foldGENE3DG3DSA:2.60.40.10coord: 179..265
e-value: 4.9E-5
score: 25.6
IPR008974TRAF-likeSUPERFAMILYSSF49599TRAF domain-likecoord: 540..661