SMED30025346

Overview
NameSMED30025346
Smed IDSMED30025346
Length (bp)2626
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30025346 (SMED30025346) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30025346 (SMED30025346) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30025346 (SMED30025346) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30025346 (SMED30025346) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30025346

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30025346 SmedASXL_010327SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
protonephridiaSMED30025346SMESG000048407.1 SMESG000002220.1 SMESG000002212.1 SMESG000001733.1 dd_Smed_v4_9947_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30025346SMESG000048407.1 dd_Smed_v4_3271_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30025346 vs. Planmine SMEST
Match: SMESG000048407.1 (SMESG000048407.1)

HSP 1 Score: 1333.55 bits (3450), Expect = 0.000e+0
Identity = 762/765 (99.61%), Postives = 764/765 (99.87%), Query Frame = 1 
Query:  112 MYNHEQNCDWIKRSKSNWSNERNVLSLTTGNCYTQEDYCGNSGLLCQSMNNNLYEYSLCXXXXXXXXXXXXXXXXXXVGSYSTTYHHQQPCQDEDWMMNCSNCTSQFNYPFSXXXXXXXXXXXXXXXXXXXXWTKNXXXXXXXXXXXXXXSNYQSNNIAGTSFNVTTAAADPLNALQNLTVSAGQSAQLMAQQNMRLNSTAFNNQIRQRSLVQGSINRPIDPLSATKSSLSQQLVQPQIPSNVVITNSNMINVNSARISTNPISMKPEICVNQNSGQQSLMLPPYPMDSCNSLMSSSQVSPMASVGKIXXXXXXXXXXXXXXLPTQHSSQIIQPTNNLFPNGAPIRRTVNSQQNLRPPSIISDXXXXXXXXXXXXXXXXYFKKLTIILENPKNVSDDEIKVWTVLKTLLENPDSIQKPLSMRHISKIEQHLMKAEQHVLQKCSNQTTNIHLEKLHPNTSQIVAQSQCRTTVQKPIVNYDEKSMKKSVYPLSNSSNENVDLNNGSINSSSLDIAFDDVCRKLINTDYKVVKRVQQNLIHFFKSRQTQYRHFLPKRVFIDPDDEAAADDTKPKHPKLEPLSVDEEFGVLDKDEDDGLDLFVNKDSISLIDENRLLHTDLNFACIDMSFCDLAXXXXXXXXXFQVTKIEEEHVSKDWFTHDSDWADSQFFSFLYIYKSLPSIVPPIKIRISADYEDSGSTDWNLNLELYQLPFDDFLNEVASLLTTNFTEAHHNYQWSGIPVLYIMSLYKRSILLAHENYLNRLSDES 2406
            MYNHEQNCDWIKRSKSNWSNERNVLSLTTGNCYTQEDYCGNSGLLCQSMNNNLYEYSLCGDKNGYYNDNYCGNDY GVGSYSTTYHHQQPCQD+DWMMNCSNCTSQFNYPFSAQYNHQQPQQHRHHQQRNAYWTKNQHPQPQMQMPGTMMSNYQSNNIAGTSFNVTTAAADPLNALQNLTVSAGQSAQLMAQQNMRL+STAFNNQIRQRSLVQGSINRPIDPLSATKSSLSQQLVQPQIPSNVVITNSNMINVNSARISTNPISMKPEICVNQNSGQQSLMLPPYPMDSCNSLMSSSQVSPMASVGKITPYSSTPSSQYQSSLPTQHSSQIIQPTNNLFPNGAPIRRTVNSQQNLRPPSIISDEEAKKVKEEVKRLKEKYFKKLTIILENPKNVSDDEIKVWTVLKTLLENPDSIQKPLSMRHISKIEQHLMKAEQHVLQKCSNQTTNIHLEKLHPNTSQIVAQSQCRTTVQKPIVNYDEKSMKKSVYPLSNSSNENVDLNNGSINSSSLDIAFDDVCRKLINTDYKVVKRVQQNLIHFFKSRQTQYRHFLPKRVFIDPDDEAAADDTKPKHPKLEPLSVDEEFGVLDKDEDDGLDLFVNKDSISLIDENRLLHTDLNFACIDMSFCDLAKELKLLEKEFQVTKIEEEHVSKDWFTHDSDWADSQFFSFLYIYKSLPSIVPPIKIRISADYEDSGSTDWNLNLELYQLPFDDFLNEVASLLTTNFTEAHHNYQWSGIPVLYIMSLYKRSILLAHENYLNRLSDES
Sbjct:    1 MYNHEQNCDWIKRSKSNWSNERNVLSLTTGNCYTQEDYCGNSGLLCQSMNNNLYEYSLCGDKNGYYNDNYCGNDYAGVGSYSTTYHHQQPCQDDDWMMNCSNCTSQFNYPFSAQYNHQQPQQHRHHQQRNAYWTKNQHPQPQMQMPGTMMSNYQSNNIAGTSFNVTTAAADPLNALQNLTVSAGQSAQLMAQQNMRLSSTAFNNQIRQRSLVQGSINRPIDPLSATKSSLSQQLVQPQIPSNVVITNSNMINVNSARISTNPISMKPEICVNQNSGQQSLMLPPYPMDSCNSLMSSSQVSPMASVGKITPYSSTPSSQYQSSLPTQHSSQIIQPTNNLFPNGAPIRRTVNSQQNLRPPSIISDEEAKKVKEEVKRLKEKYFKKLTIILENPKNVSDDEIKVWTVLKTLLENPDSIQKPLSMRHISKIEQHLMKAEQHVLQKCSNQTTNIHLEKLHPNTSQIVAQSQCRTTVQKPIVNYDEKSMKKSVYPLSNSSNENVDLNNGSINSSSLDIAFDDVCRKLINTDYKVVKRVQQNLIHFFKSRQTQYRHFLPKRVFIDPDDEAAADDTKPKHPKLEPLSVDEEFGVLDKDEDDGLDLFVNKDSISLIDENRLLHTDLNFACIDMSFCDLAKELKLLEKEFQVTKIEEEHVSKDWFTHDSDWADSQFFSFLYIYKSLPSIVPPIKIRISADYEDSGSTDWNLNLELYQLPFDDFLNEVASLLTTNFTEAHHNYQWSGIPVLYIMSLYKRSILLAHENYLNRLSDES 765
[Close]
BLAST of SMED30025346 vs. Planmine SMEST
Match: SMESG000048407.1 (SMESG000048407.1)

HSP 1 Score: 1140.56 bits (2949), Expect = 0.000e+0
Identity = 631/632 (99.84%), Postives = 632/632 (100.00%), Query Frame = 1 
Query:  511 TKNXXXXXXXXXXXXXXSNYQSNNIAGTSFNVTTAAADPLNALQNLTVSAGQSAQLMAQQNMRLNSTAFNNQIRQRSLVQGSINRPIDPLSATKSSLSQQLVQPQIPSNVVITNSNMINVNSARISTNPISMKPEICVNQNSGQQSLMLPPYPMDSCNSLMSSSQVSPMASVGKIXXXXXXXXXXXXXXLPTQHSSQIIQPTNNLFPNGAPIRRTVNSQQNLRPPSIISDXXXXXXXXXXXXXXXXYFKKLTIILENPKNVSDDEIKVWTVLKTLLENPDSIQKPLSMRHISKIEQHLMKAEQHVLQKCSNQTTNIHLEKLHPNTSQIVAQSQCRTTVQKPIVNYDEKSMKKSVYPLSNSSNENVDLNNGSINSSSLDIAFDDVCRKLINTDYKVVKRVQQNLIHFFKSRQTQYRHFLPKRVFIDPDDEAAADDTKPKHPKLEPLSVDEEFGVLDKDEDDGLDLFVNKDSISLIDENRLLHTDLNFACIDMSFCDLAXXXXXXXXXFQVTKIEEEHVSKDWFTHDSDWADSQFFSFLYIYKSLPSIVPPIKIRISADYEDSGSTDWNLNLELYQLPFDDFLNEVASLLTTNFTEAHHNYQWSGIPVLYIMSLYKRSILLAHENYLNRLSDES 2406
            TKNQHPQPQMQMPGTMMSNYQSNNIAGTSFNVTTAAADPLNALQNLTVSAGQSAQLMAQQNMRL+STAFNNQIRQRSLVQGSINRPIDPLSATKSSLSQQLVQPQIPSNVVITNSNMINVNSARISTNPISMKPEICVNQNSGQQSLMLPPYPMDSCNSLMSSSQVSPMASVGKITPYSSTPSSQYQSSLPTQHSSQIIQPTNNLFPNGAPIRRTVNSQQNLRPPSIISDEEAKKVKEEVKRLKEKYFKKLTIILENPKNVSDDEIKVWTVLKTLLENPDSIQKPLSMRHISKIEQHLMKAEQHVLQKCSNQTTNIHLEKLHPNTSQIVAQSQCRTTVQKPIVNYDEKSMKKSVYPLSNSSNENVDLNNGSINSSSLDIAFDDVCRKLINTDYKVVKRVQQNLIHFFKSRQTQYRHFLPKRVFIDPDDEAAADDTKPKHPKLEPLSVDEEFGVLDKDEDDGLDLFVNKDSISLIDENRLLHTDLNFACIDMSFCDLAKELKLLEKEFQVTKIEEEHVSKDWFTHDSDWADSQFFSFLYIYKSLPSIVPPIKIRISADYEDSGSTDWNLNLELYQLPFDDFLNEVASLLTTNFTEAHHNYQWSGIPVLYIMSLYKRSILLAHENYLNRLSDES
Sbjct:   59 TKNQHPQPQMQMPGTMMSNYQSNNIAGTSFNVTTAAADPLNALQNLTVSAGQSAQLMAQQNMRLSSTAFNNQIRQRSLVQGSINRPIDPLSATKSSLSQQLVQPQIPSNVVITNSNMINVNSARISTNPISMKPEICVNQNSGQQSLMLPPYPMDSCNSLMSSSQVSPMASVGKITPYSSTPSSQYQSSLPTQHSSQIIQPTNNLFPNGAPIRRTVNSQQNLRPPSIISDEEAKKVKEEVKRLKEKYFKKLTIILENPKNVSDDEIKVWTVLKTLLENPDSIQKPLSMRHISKIEQHLMKAEQHVLQKCSNQTTNIHLEKLHPNTSQIVAQSQCRTTVQKPIVNYDEKSMKKSVYPLSNSSNENVDLNNGSINSSSLDIAFDDVCRKLINTDYKVVKRVQQNLIHFFKSRQTQYRHFLPKRVFIDPDDEAAADDTKPKHPKLEPLSVDEEFGVLDKDEDDGLDLFVNKDSISLIDENRLLHTDLNFACIDMSFCDLAKELKLLEKEFQVTKIEEEHVSKDWFTHDSDWADSQFFSFLYIYKSLPSIVPPIKIRISADYEDSGSTDWNLNLELYQLPFDDFLNEVASLLTTNFTEAHHNYQWSGIPVLYIMSLYKRSILLAHENYLNRLSDES 690
[Close]
BLAST of SMED30025346 vs. Planmine SMEST
Match: SMESG000015763.1 (SMESG000015763.1)

HSP 1 Score: 91.2781 bits (225), Expect = 7.239e-19
Identity = 68/186 (36.56%), Postives = 97/186 (52.15%), Query Frame = 1 
Query: 1852 DEEFGVLDKDEDDGLDLFVNKDSISLIDENRLLHTDLNFACIDMSFCDLAXXXXXXXXXFQVTKIEEEHVSKDWFTHDSDWADSQFFSFLYIYKSLPSIVPPIKIRISADYEDSGSTDWNLNLELYQL-PFDD---FLNEVASLLTTNFTEAHHNYQWSGIPVLYIMSLYKRSILLAHENYLNRLS 2397
            D EF  +D    DG ++   K +    D N LL  D N+   +    D+  E+KLLEKEF++ +IE +  ++ W + DS+W +S F+   Y   + PS +P I +RI  DYE S S DWNLN   YQ  P  +   FL  +   L   F+       W+ +P L I+S+YKRSI +   NY  R S
Sbjct:  336 DHEFDYIDNLCVDGENIQPYKMANRSFDHNGLLRIDRNWRFCETDSFDIKSEIKLLEKEFEINQIEPQRRTEAWVSFDSEWENSTFYGIRYRNNNFPSELPSIIVRIPEDYEQSYSADWNLNYNEYQCHPNSEAHLFLETILMSLRNEFSNLSSQ-SWNIVPFLDIVSIYKRSIQIGLLNYECRKS 520
[Close]
BLAST of SMED30025346 vs. Planmine SMEST
Match: SMESG000015774.1 (SMESG000015774.1)

HSP 1 Score: 91.2781 bits (225), Expect = 7.565e-19
Identity = 68/186 (36.56%), Postives = 97/186 (52.15%), Query Frame = 1 
Query: 1852 DEEFGVLDKDEDDGLDLFVNKDSISLIDENRLLHTDLNFACIDMSFCDLAXXXXXXXXXFQVTKIEEEHVSKDWFTHDSDWADSQFFSFLYIYKSLPSIVPPIKIRISADYEDSGSTDWNLNLELYQL-PFDD---FLNEVASLLTTNFTEAHHNYQWSGIPVLYIMSLYKRSILLAHENYLNRLS 2397
            D EF  +D    DG ++   K +    D N LL  D N+   +    D+  E+KLLEKEF++ +IE +  ++ W + DS+W +S F+   Y   + PS +P I +RI  DYE S S DWNLN   YQ  P  +   FL  +   L   F+       W+ +P L I+S+YKRSI +   NY  R S
Sbjct:  336 DHEFDYIDNLCVDGENIQPYKMANRSFDHNGLLRIDRNWRFCETDSFDIKSEIKLLEKEFEINQIEPQRRTEAWVSFDSEWENSTFYGIRYRNNNFPSELPSIIVRIPEDYEQSYSADWNLNYNEYQCHPNSEAHLFLETILMSLRNEFSNLSSQ-SWNIVPFLDIVSIYKRSIQIGLLNYECRKS 520
[Close]
BLAST of SMED30025346 vs. Planmine SMEST
Match: SMESG000015798.1 (SMESG000015798.1)

HSP 1 Score: 62.7734 bits (151), Expect = 1.360e-10
Identity = 39/121 (32.23%), Postives = 64/121 (52.89%), Query Frame = 1 
Query: 2029 FQVTKIEEEHVSKDWFTHDSDWADSQFFSFLYIYKSLPSIVPPIKIRISADYEDSGSTDWNLNLELYQLPF----DDFLNEVASLLTTNFTEAHHNYQWSGIPVLYIMSLYKRSILLAHEN 2379
            F++T+I  +   +DW   +S+W +S+F+   Y +  LP+ + PI IRIS +YE S   D+NLNL+ Y        D+FL+ +     +  T         G+ +L I+  YK+S+  A  N
Sbjct:   96 FEITEIGPQLEPEDWTKFESNWENSRFYRIKYKHDKLPTDIIPITIRISENYERSQMVDFNLNLKEYPKKIVSEADEFLSSIKDAYLSCLTLK------DGLSLLAILGKYKQSVQAALGN 210
The following BLAST results are available for this feature:
BLAST of SMED30025346 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025346 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025346 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025346 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025346 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025346 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025346 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025346 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025346 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025346 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025346 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025346 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025346 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025346 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000048407.10.000e+099.61SMESG000048407.1[more]
SMESG000048407.10.000e+099.84SMESG000048407.1[more]
SMESG000015763.17.239e-1936.56SMESG000015763.1[more]
SMESG000015774.17.565e-1936.56SMESG000015774.1[more]
SMESG000015798.11.360e-1032.23SMESG000015798.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30025346 ID=SMED30025346|Name=SMED30025346|organism=Schmidtea mediterranea sexual|type=transcript|length=2626bp
ATTTATCCATGCTCTTATTGCCACTGAAATTTGATTGGCCGATATCTATT
TCATAAATAGATTTTCCATGCTTTTATTCATTAAATTGGAAAAATCGAAA
TTATTGACTAAATGTACAATCATGAACAGAACTGCGATTGGATTAAGCGA
TCAAAATCTAATTGGAGTAATGAACGGAATGTTTTGTCATTGACAACTGG
AAATTGCTATACTCAAGAAGATTATTGTGGAAATTCTGGATTGTTGTGCC
AAAGCATGAATAACAATTTGTATGAGTATTCTTTGTGCGGAGATAAAAAT
GGGTATTATAACGATAATTACTGTGGGAATGATTATACTGGAGTTGGCAG
TTATTCGACAACATATCACCACCAGCAACCTTGTCAAGATGAAGATTGGA
TGATGAATTGTAGTAATTGCACTTCCCAATTCAATTATCCATTCTCAGCA
CAGTATAATCACCAACAGCCCCAGCAGCATCGTCATCATCAGCAACGCAA
CGCTTATTGGACCAAGAATCAACATCCACAGCCACAAATGCAAATGCCCG
GAACAATGATGTCGAATTATCAGTCGAACAATATCGCTGGCACTTCATTC
AATGTCACAACAGCCGCTGCCGATCCGTTAAATGCGTTACAAAATCTGAC
TGTCAGTGCTGGCCAATCAGCGCAATTAATGGCCCAGCAAAATATGAGAC
TCAACTCGACAGCGTTCAACAATCAAATCCGTCAGAGATCTCTAGTCCAA
GGTTCCATTAACCGGCCAATAGATCCGCTCTCCGCTACGAAATCTTCATT
GTCTCAACAGCTAGTCCAACCTCAGATTCCATCAAACGTTGTTATCACTA
ACTCCAATATGATCAACGTCAACAGTGCAAGGATATCAACCAATCCAATC
TCTATGAAGCCCGAAATTTGTGTTAATCAAAATAGTGGACAGCAGTCTCT
GATGCTCCCTCCATATCCTATGGATTCCTGTAATTCTTTGATGAGCAGCT
CGCAAGTCTCTCCGATGGCATCTGTCGGGAAAATCACTCCATATAGTTCC
ACGCCATCGTCTCAGTACCAATCAAGTTTACCTACTCAACATTCATCTCA
AATAATTCAACCAACGAACAACTTATTCCCAAACGGAGCTCCGATCAGAC
GAACTGTGAACTCTCAACAAAACTTGCGACCGCCAAGTATTATTTCTGAC
GAGGAGGCGAAAAAGGTCAAAGAAGAAGTTAAAAGGCTTAAAGAAAAATA
TTTTAAAAAGTTAACGATTATTCTTGAAAATCCGAAAAATGTCTCAGATG
ATGAAATCAAAGTTTGGACAGTTCTCAAAACTCTTCTGGAGAATCCGGAT
TCCATACAGAAACCTCTATCAATGAGGCACATTAGTAAAATTGAACAGCA
TTTGATGAAAGCGGAACAGCACGTGTTGCAAAAGTGTTCAAATCAAACAA
CGAATATACATTTAGAAAAACTCCACCCAAATACATCACAAATTGTTGCT
CAAAGTCAGTGTCGAACGACCGTTCAAAAACCAATAGTAAATTATGATGA
AAAATCCATGAAGAAATCAGTTTACCCTCTGTCGAATAGCTCAAACGAGA
ATGTCGATTTAAACAATGGTTCAATAAATTCGAGTTCACTCGATATCGCT
TTCGATGATGTCTGTCGAAAACTGATTAACACTGACTACAAAGTTGTGAA
ACGAGTTCAACAAAATCTCATTCATTTCTTCAAATCAAGGCAGACGCAAT
ATAGACATTTTTTGCCGAAACGAGTGTTTATTGATCCGGACGATGAAGCG
GCTGCAGACGACACCAAACCGAAACATCCTAAATTAGAGCCGCTTTCAGT
CGATGAAGAATTTGGCGTCCTGGACAAGGATGAGGATGACGGATTAGATT
TATTTGTAAATAAAGATTCGATAAGTTTGATAGACGAAAATAGGTTATTG
CACACAGATCTAAATTTTGCTTGTATTGATATGAGTTTTTGTGACTTGGC
GAAGGAATTGAAATTATTGGAAAAGGAATTCCAAGTAACGAAAATCGAAG
AAGAACATGTTTCCAAAGATTGGTTTACCCATGATTCTGATTGGGCAGAC
AGTCAATTTTTTTCTTTTTTATATATTTACAAGAGTTTACCTTCAATTGT
GCCGCCAATCAAGATTCGAATATCGGCCGACTATGAAGACAGTGGATCTA
CAGATTGGAATCTGAATTTGGAACTCTATCAATTACCTTTTGATGATTTC
CTGAATGAGGTCGCAAGCTTGCTCACGACTAACTTCACCGAAGCCCACCA
CAATTATCAATGGTCTGGAATACCGGTTCTGTATATTATGAGTCTTTATA
AACGGTCAATCCTCCTAGCACATGAAAACTATCTCAATCGATTAAGTGAT
GAAAGTTGAAATACTCGGGAAAATGATTTGAAAATAACAACAGAATTTAT
TAATAAATTTTTTTCAACACATTGTTGGCTTCTGTGATGAAATTCATTTC
CAATAAAATCTCCATCCAGTTAATTAGGAATTGGCTGAACTGTTTGCTGT
CTATTGTAAATTCGGTTTCCTTCGTTGTTAAATTTATTTCATACTTTCTT
CTTGGACTGGCACATTTCCAATCGTT
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protein sequence of SMED30025346-orf-1

>SMED30025346-orf-1 ID=SMED30025346-orf-1|Name=SMED30025346-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=766bp
MYNHEQNCDWIKRSKSNWSNERNVLSLTTGNCYTQEDYCGNSGLLCQSMN
NNLYEYSLCGDKNGYYNDNYCGNDYTGVGSYSTTYHHQQPCQDEDWMMNC
SNCTSQFNYPFSAQYNHQQPQQHRHHQQRNAYWTKNQHPQPQMQMPGTMM
SNYQSNNIAGTSFNVTTAAADPLNALQNLTVSAGQSAQLMAQQNMRLNST
AFNNQIRQRSLVQGSINRPIDPLSATKSSLSQQLVQPQIPSNVVITNSNM
INVNSARISTNPISMKPEICVNQNSGQQSLMLPPYPMDSCNSLMSSSQVS
PMASVGKITPYSSTPSSQYQSSLPTQHSSQIIQPTNNLFPNGAPIRRTVN
SQQNLRPPSIISDEEAKKVKEEVKRLKEKYFKKLTIILENPKNVSDDEIK
VWTVLKTLLENPDSIQKPLSMRHISKIEQHLMKAEQHVLQKCSNQTTNIH
LEKLHPNTSQIVAQSQCRTTVQKPIVNYDEKSMKKSVYPLSNSSNENVDL
NNGSINSSSLDIAFDDVCRKLINTDYKVVKRVQQNLIHFFKSRQTQYRHF
LPKRVFIDPDDEAAADDTKPKHPKLEPLSVDEEFGVLDKDEDDGLDLFVN
KDSISLIDENRLLHTDLNFACIDMSFCDLAKELKLLEKEFQVTKIEEEHV
SKDWFTHDSDWADSQFFSFLYIYKSLPSIVPPIKIRISADYEDSGSTDWN
LNLELYQLPFDDFLNEVASLLTTNFTEAHHNYQWSGIPVLYIMSLYKRSI
LLAHENYLNRLSDES*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0002109X1 cell