SMED30025328

Overview
NameSMED30025328
Smed IDSMED30025328
Length (bp)780
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30025328

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
testisSMED30025328h1SMcG0014904 Contig1152_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
early male germ cellSMED30025328h1SMcG0014904 Contig1152_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism asexual adult cDNA to DNA expression microarray evidence
testis primordiumSMED30025328h1SMcG0014904 Contig1152_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism juvenile stagecDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Alignments
SMED30025328 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000960:25924..26703 +3900
Homology
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BLAST of SMED30025328 vs. Planmine SMEST
Match: SMESG000012110.1 (SMESG000012110.1)

HSP 1 Score: 381.333 bits (978), Expect = 5.082e-136
Identity = 196/196 (100.00%), Postives = 196/196 (100.00%), Query Frame = 3 
Query:   69 MKNQEKIPENPMPDEMGSKPPASIEELWNSTARAINKNMREIRGQLRLNNQLIEQMKSGILKTLSYIRESSQKQFCRTPSYRTDSALHNVHKAGTSFKPVNRNIASRPMSVNMSNYDQGSARSQCRTYSRPGHMKRPKMNFVNPYDIDNSNSVTHPIKCSRGHTNFNIETNEECEGESTVLXXXXXXXXXXXXXYV 656
            MKNQEKIPENPMPDEMGSKPPASIEELWNSTARAINKNMREIRGQLRLNNQLIEQMKSGILKTLSYIRESSQKQFCRTPSYRTDSALHNVHKAGTSFKPVNRNIASRPMSVNMSNYDQGSARSQCRTYSRPGHMKRPKMNFVNPYDIDNSNSVTHPIKCSRGHTNFNIETNEECEGESTVLSDSEDEMDESSMMYV
Sbjct:    1 MKNQEKIPENPMPDEMGSKPPASIEELWNSTARAINKNMREIRGQLRLNNQLIEQMKSGILKTLSYIRESSQKQFCRTPSYRTDSALHNVHKAGTSFKPVNRNIASRPMSVNMSNYDQGSARSQCRTYSRPGHMKRPKMNFVNPYDIDNSNSVTHPIKCSRGHTNFNIETNEECEGESTVLSDSEDEMDESSMMYV 196
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BLAST of SMED30025328 vs. Planmine SMEST
Match: SMESG000012064.1 (SMESG000012064.1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.508e-13
Identity = 28/54 (51.85%), Postives = 45/54 (83.33%), Query Frame = 3 
Query:  129 PASIEELWNSTARAINKNMREIRGQLRLNNQLIEQMKSGILKTLSYIRESSQKQ 290
            P ++E+LWNST+RAINKNM +IR QL+ NN++I+++++ I +TLS ++ S+Q Q
Sbjct:    9 PTNLEQLWNSTSRAINKNMVDIRTQLKANNEMIKEIRNSIFQTLSVMKNSNQNQ 62
The following BLAST results are available for this feature:
BLAST of SMED30025328 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025328 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025328 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025328 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025328 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025328 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025328 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025328 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025328 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025328 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025328 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025328 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025328 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025328 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000012110.15.082e-136100.00SMESG000012110.1[more]
SMESG000012064.11.508e-1351.85SMESG000012064.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30025328 ID=SMED30025328|Name=SMED30025328|organism=Schmidtea mediterranea sexual|type=transcript|length=780bp
GTAAATAGTAAATAATTAAATAATACAATCAATAGTATTTGAAATTCAAA
AAACAGTTAGATACAAGAATGAAAAACCAAGAAAAAATTCCAGAAAACCC
AATGCCTGATGAAATGGGCTCAAAGCCTCCAGCGTCAATAGAAGAATTGT
GGAATTCTACTGCTCGGGCTATTAACAAAAACATGCGAGAAATTCGCGGT
CAATTACGATTAAATAATCAGTTAATTGAACAAATGAAATCGGGAATATT
AAAAACCCTGTCATACATTCGTGAGAGTAGTCAGAAGCAGTTCTGCAGGA
CTCCAAGTTACCGGACTGACAGCGCCCTTCATAATGTTCATAAGGCCGGA
ACCTCATTTAAACCAGTGAATCGTAACATAGCATCAAGGCCGATGTCAGT
CAACATGTCTAATTATGACCAGGGATCCGCCCGCTCTCAGTGTAGGACAT
ACTCAAGACCTGGCCACATGAAACGCCCTAAAATGAATTTCGTAAATCCT
TATGATATTGATAATTCAAATTCAGTAACGCATCCCATAAAATGCTCAAG
GGGCCACACGAATTTCAATATCGAGACGAATGAAGAGTGTGAAGGAGAAA
GCACAGTTTTGAGTGATTCAGAAGATGAAATGGATGAGTCCTCCATGATG
TATGTCTAACAGTAAATGCGAATGCATTAAGGAATTAGGCACTTTTTTAA
AATAAGGATGAAGTAAATGGGTAACCTTTAAATCCATAAAATTACTGAGT
TTATAAAAAATAAATGTATTCAAATGTTTG
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protein sequence of SMED30025328-orf-1

>SMED30025328-orf-1 ID=SMED30025328-orf-1|Name=SMED30025328-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=197bp
MKNQEKIPENPMPDEMGSKPPASIEELWNSTARAINKNMREIRGQLRLNN
QLIEQMKSGILKTLSYIRESSQKQFCRTPSYRTDSALHNVHKAGTSFKPV
NRNIASRPMSVNMSNYDQGSARSQCRTYSRPGHMKRPKMNFVNPYDIDNS
NSVTHPIKCSRGHTNFNIETNEECEGESTVLSDSEDEMDESSMMYV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000208testis
PLANA:0002030early male germ cell
PLANA:0003304testis primordium
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 176..190
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 174..196
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20