Universal stress protein MSMEG_3950/MSMEI_3859

Overview
NameUniversal stress protein MSMEG_3950/MSMEI_3859
Smed IDSMED30025317
Length (bp)1062
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Universal stress protein MSMEG_3950/MSMEI_3859 (SMED30025317) t-SNE clustered cells

Violin plots show distribution of expression levels for Universal stress protein MSMEG_3950/MSMEI_3859 (SMED30025317) in cells (dots) of each of the 12 neoblast clusters.

 

back to top


 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Universal stress protein MSMEG_3950/MSMEI_3859 (SMED30025317) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Universal stress protein MSMEG_3950/MSMEI_3859 (SMED30025317) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top


 

Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020




SMED30025317

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 13

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30025317h1SMcG0022556 Contig370GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
Smed sexual biotypeSMED30025317h1SMcG0022554 Contig17787uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025317h1SMcG0022554 Contig17787newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025317h1SMcG0022556 Contig17787uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025317h1SMcG0022556 Contig17787newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
protonephridiaSMED30025317h1SMcG0022556 dd_Smed_v4_402_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30025317h1SMcG0022556 dd_Smed_v4_402_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30025317h1SMcG0022556 dd_Smed_v4_402_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30025317h1SMcG0022556 dd_Smed_v4_402_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025317h1SMcG0022553 Contig17787uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025317h1SMcG0022553 Contig17787newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025317h1SMcG0022555 Contig17787uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025317h1SMcG0022555 Contig17787newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. UniProt/SwissProt
Match: sp|A0QZA1|Y3950_MYCS2 (Universal stress protein MSMEG_3950/MSMEI_3859 OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) OX=246196 GN=MSMEG_3950 PE=1 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 7.097e-8
Identity = 41/139 (29.50%), Postives = 69/139 (49.64%), Query Frame = 3
Query:  378 IMIPIDSSEHSKRAFRWYIKNMKQPNDLVLFIHVVEPVYAT-PAVGMMEPPPIVLDDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIHVDTKPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHA 791
            I++ +DSS  S  A RW  +     +  +  +HV+ PV  + PA   M     VL+        AI + +K+  + + E    GL  +  I  ++     L+ ++K  Q  ++++GNRG+G L R  LGS S  +LH+A
Sbjct:   10 ILVGVDSSAESDAAVRWAAREASLHDAPITLMHVIAPVVVSWPAGPYM---ATVLECQEENARHAIEQAQKVVADCLGETH--GLTVQTEIRKESV-ARTLIDASKSAQ--MVVVGNRGMGALGRVLLGSTSTSLLHYA 140          
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. UniProt/SwissProt
Match: sp|P74148|Y1388_SYNY3 (Universal stress protein Sll1388 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=sll1388 PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 7.325e-8
Identity = 36/155 (23.23%), Postives = 72/155 (46.45%), Query Frame = 3
Query:  363 MAKRSIMIPIDSSEHSKRAFRWYIK-NMKQPNDLVLF---------IHVVEPVYATPAVGMMEPPPIVLDDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIHVDTKPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHAHI 797
            M    I++ +D SE +K   +  I    K+ + L++F         + +    Y   A+G  +        +   +E+   E ++  Q  + + ++ G+  +  + V  +PG  +   AK+  AD++++G RG+  L   FLGSVS +++HH   
Sbjct:    1 MGYGKILVALDRSELAKEVLQQAIALGQKESSQLMVFYCIPVDSQDLSIYPSFYGEAAIGFSQI-------IKEHLEEQQTEAREWLQSIVQQVQEDGVACEWDVKVG-EPGRWIRDMAKNWDADLVVLGRRGLKGLAEVFLGSVSSYVIHHVQC 147          
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. UniProt/SwissProt
Match: sp|Q8LGG8|USPAL_ARATH (Universal stress protein A-like protein OS=Arabidopsis thaliana OX=3702 GN=At3g01520 PE=1 SV=2)

HSP 1 Score: 51.9878 bits (123), Expect = 8.917e-7
Identity = 34/143 (23.78%), Postives = 67/143 (46.85%), Query Frame = 3
Query:  408 SKRAFRWYIKNMKQPNDL---VLFIHV----------VEPVYATPAVGMMEPPPIVLDDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIHVDTKPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHAHI 797
             KRAF W ++ + + N     +L +HV          V+ +YA+P            +D   M +    +G  L + F+++  + G+  +A+I     P   + +  K  + D +++G+RG+G  ++ F+G+VS   + HA  
Sbjct:   27 CKRAFEWTLEKIVRSNTSDFKILLLHVQVVDEDGFDDVDSIYASP------------EDFRDMRQSNKAKGLHLLEFFVNKCHEIGVGCEAWIKT-GDPKDVICQEVKRVRPDFLVVGSRGLGRFQKVFVGTVSAFCVKHAEC 156          
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. UniProt/SwissProt
Match: sp|Q57951|Y531_METJA (Universal stress protein MJ0531 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0531 PE=3 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 8.990e-7
Identity = 41/144 (28.47%), Postives = 73/144 (50.69%), Query Frame = 3
Query:  372 RSIMIPIDSSEHSKRAFRWYIKNMKQPNDLVLFIHVVEPVYATPAVGMMEPPPIVLDDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIH---VDTKPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHAH 794
            + I+IP D S+ S  A +  I   K+ +  V  I+VV+    +P VG+  P     + +S ++++        GQE + + K    E    IH   ++  P   +V+ A+  +AD+I++G  G   L R  LGSV++ ++ +AH
Sbjct:   27 KKIVIPTDGSDVSLEAAKHAINIAKEFDAEVYAIYVVD---VSPFVGL--PAEGSWELISELLKEE-------GQEALKKVKKMAEEWGVKIHTEMLEGVPANEIVEFAEKKKADLIVMGTTGKTGLERILLGSVAERVIKNAH 158          
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. TrEMBL
Match: A0A448X5I9 (Usp domain-containing protein OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS22031 PE=4 SV=1)

HSP 1 Score: 214.157 bits (544), Expect = 2.773e-65
Identity = 97/143 (67.83%), Postives = 125/143 (87.41%), Query Frame = 3
Query:  372 RSIMIPIDSSEHSKRAFRWYIKNMKQPNDLVLFIHVVEPVYATPAVGM-MEPPPIVLDDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIHVDTKPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHAHI 797
            R+I+IPID SEHS RAF WYI+N+K+  D V  IHV+EPVY TPAVG+ ME PP+++DDM+R+ME++I  GKKLGQ+FM +AK+ G++ KAF+HVDTKPG ALVKS+ DH+AD+I++GNRG+GTLRRTFLGSVSDH+LHH+HI
Sbjct:   33 RNILIPIDGSEHSDRAFVWYIENLKKAGDCVHLIHVIEPVYTTPAVGLAMESPPLLVDDMTRVMEESIESGKKLGQQFMRKAKEFGVDSKAFLHVDTKPGHALVKSSIDHKADIIVMGNRGLGTLRRTFLGSVSDHVLHHSHI 175          
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. TrEMBL
Match: A0A0R3U2V2 (Usp domain-containing protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS832 PE=4 SV=1)

HSP 1 Score: 204.912 bits (520), Expect = 5.542e-62
Identity = 88/143 (61.54%), Postives = 124/143 (86.71%), Query Frame = 3
Query:  372 RSIMIPIDSSEHSKRAFRWYIKNMKQPNDLVLFIHVVEPVYATPAVGM-MEPPPIVLDDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIHVDTKPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHAHI 797
            R ++ PID+S++ +RAF+WY  N+++PNDLV F+HV+EPVY TPA+G+ ME PP+++DDM+R+ME++I  GKKLGQ++M  AKD  +E KAF+HVDTKPGAA+VKSA DH+AD+I+IG+RG+G ++RTFLGSVSD+I+H+A I
Sbjct:    5 RRVLFPIDASDNCQRAFKWYEVNLRKPNDLVYFVHVIEPVYTTPAIGLAMESPPLMVDDMTRVMEESIAAGKKLGQKYMQMAKDENIECKAFLHVDTKPGAAIVKSATDHKADIIVIGSRGLGAIKRTFLGSVSDYIVHNASI 147          
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. TrEMBL
Match: A0A183QRF3 (Usp domain-containing protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 203.756 bits (517), Expect = 1.845e-61
Identity = 92/145 (63.45%), Postives = 121/145 (83.45%), Query Frame = 3
Query:  366 AKRSIMIPIDSSEHSKRAFRWYIKNMKQPNDLVLFIHVVEPVYATPAVGM-MEPPPIVLDDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIHVDTKPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHAHI 797
            + R I+IPID S+H  RAFRWY++NMK+  D + F+HV+EPVY TPA+GM ME PPI   DM+R+ME++I +GKKLGQ++MHEAK   L  KAF+HVDTKPG++LVK+  DH+A+VI++GNRG+G +RRTFLGSVSD++LHHAHI
Sbjct:    7 SSRVILIPIDGSDHCDRAFRWYLENMKRDTDCITFVHVIEPVYNTPAIGMTMESPPI--PDMTRVMEESIEQGKKLGQKYMHEAKSYKLNAKAFLHVDTKPGSSLVKAISDHKANVILMGNRGLGAIRRTFLGSVSDYVLHHAHI 149          
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. TrEMBL
Match: A0A094ZGI8 (Universal stress protein OS=Schistosoma haematobium OX=6185 GN=MS3_01096 PE=4 SV=1)

HSP 1 Score: 202.601 bits (514), Expect = 4.248e-61
Identity = 92/145 (63.45%), Postives = 121/145 (83.45%), Query Frame = 3
Query:  366 AKRSIMIPIDSSEHSKRAFRWYIKNMKQPNDLVLFIHVVEPVYATPAVGM-MEPPPIVLDDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIHVDTKPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHAHI 797
            + R I+IPID S+H  RAFRWY++NMK+  D + F+HV+EPVY TPA+GM ME PPI   DM+R+ME++I +GKKLGQ++MHEAK   L  KAF+HVDTKPG++LVK+  DH+A+VI++GNRG+G +RRTFLGSVSD++LHHAHI
Sbjct:    7 SSRVILIPIDGSDHCDRAFRWYLENMKRDTDCITFVHVIEPVYNTPAIGMTMESPPI--PDMTRVMEESIEQGKKLGQKYMHEAKSHKLNAKAFLHVDTKPGSSLVKAISDHKANVILMGNRGLGAIRRTFLGSVSDYVLHHAHI 149          
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. TrEMBL
Match: A0A183K3A8 (Usp domain-containing protein OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS9473 PE=4 SV=1)

HSP 1 Score: 202.601 bits (514), Expect = 4.683e-61
Identity = 92/145 (63.45%), Postives = 121/145 (83.45%), Query Frame = 3
Query:  366 AKRSIMIPIDSSEHSKRAFRWYIKNMKQPNDLVLFIHVVEPVYATPAVGM-MEPPPIVLDDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIHVDTKPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHAHI 797
            + R I+IPID S+H  RAFRWY++NMK+  D + F+HV+EPVY TPA+GM ME PPI   DM+R+ME++I +GKKLGQ++MHEAK   L  KAF+HVDTKPG++LVK+  DH+A+VI++GNRG+G +RRTFLGSVSD++LHHAHI
Sbjct:    7 SSRVILIPIDGSDHCDRAFRWYLENMKRDTDCITFVHVIEPVYNTPAIGMTMESPPI--PDMTRVMEESIEQGKKLGQKYMHEAKSHKLNAKAFLHVDTKPGSSLVKAISDHKANVILMGNRGLGAIRRTFLGSVSDYVLHHAHI 149          
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. Ensembl Nematostella
Match: EDO45800 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRQ2])

HSP 1 Score: 110.153 bits (274), Expect = 2.705e-29
Identity = 53/142 (37.32%), Postives = 86/142 (60.56%), Query Frame = 3
Query:  372 RSIMIPIDSSEHSKRAFRWYIKNMKQPNDLVLFIHVVEPVYATPAVGMMEPPPIVLDDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIHVDTKPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHAHI 797
            R I++PIDSS+HS+ AF WY+ NM    D ++ +HV++       V       +V D+    M   + E K L +++  +A+   L+ K  +    KPG  + + +KD   D+I++G+RG+G++RRT LGSVSD++LHHAH+
Sbjct:   10 RRILLPIDSSKHSEDAFEWYVNNMHHEEDELILVHVLDSAAIQTRVS---SHGLVDDEFKNEMNKGLKEVKALEEKYKTKAETASLKAKIEVR-GGKPGETICQCSKDEHCDLILMGSRGLGSIRRTILGSVSDYVLHHAHV 147          
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. Ensembl Nematostella
Match: EDO44746 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUS8])

HSP 1 Score: 107.457 bits (267), Expect = 2.922e-28
Identity = 52/143 (36.36%), Postives = 88/143 (61.54%), Query Frame = 3
Query:  369 KRSIMIPIDSSEHSKRAFRWYIKNMKQPNDLVLFIHVVEPVYATPAVGMMEPPPIVLDDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIHVDTKPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHAHI 797
            KR ++IP+D S+HS+RAF WY +++ +P D VL IH  E     P+        ++ D+ ++ +++ I   KKL +E+  + K+ G+  + F     +PG  + + AKD  A  +++G+RG GT+RRT LGSVSD+ +HH+ +
Sbjct:    6 KRRVVIPVDGSQHSERAFNWYRQHVHEPGDEVLIIHTQEQP-TIPSSPYAYGGTVLPDEWNKAVDECIVNAKKLIEEYNKKCKEQGMTCRLF-KGSGQPGETICQLAKDLSAKHVVMGSRGCGTIRRTLLGSVSDYCVHHSSV 146          
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. Ensembl Nematostella
Match: EDO33143 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7ST34])

HSP 1 Score: 97.4413 bits (241), Expect = 1.332e-24
Identity = 51/148 (34.46%), Postives = 85/148 (57.43%), Query Frame = 3
Query:  363 MAKRSIMIPIDSSEHSKRAFRWYIKNMKQPNDLVLFIHVVEPVYATPAVGMMEPPPIVL---DDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIHVDTKPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHAHI 797
            M  R I+IP+D SEHS+RAF WY + +  P D VL +H +E     P V +    P V    ++ S M+++   + + + + +    K+  L  +  + V    G  + + A+D  A++I++G RG G +RRT LGSVSD+++HH+H+
Sbjct:    1 MEGRKILIPVDGSEHSERAFDWYAELLHSPGDEVLVVHCIE----LPPVPLEHQFPFVFAYYEEWSAMVKETREQHEAMLRSYEDICKEKKLHYEIMMVVGKPAGDVICQVARDVSANLIVLGTRGQGMIRRTILGSVSDYVVHHSHL 144          
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. Ensembl Nematostella
Match: EDO38142 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SDN9])

HSP 1 Score: 98.2117 bits (243), Expect = 1.493e-24
Identity = 60/156 (38.46%), Postives = 87/156 (55.77%), Query Frame = 3
Query:  366 AKRSIMIPIDSSEHSKRAFRWYIKNMKQPNDLVLFIHVVEPVYATPAVGMMEPPPI---VLDDMSRMMEDAINEGKKL----GQEFMHEAKDTGLEPKAFIHVDT-----KPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHAHI 797
             KR +++ +D S +S RA++WY  N+ Q  DL+L IH  E +   PA     P P      ++ S +++ A +E K L    G++   +      E K  IH        KPG  + K A+D  A +II+G+RG+GTLRRTFLGS SD+ +HHAHI
Sbjct:    8 CKRKVVLALDGSVNSMRAYQWYWDNIYQEGDLLLVIHAFE-LPTMPAA----PYPYGFAYYEEWSSLVQKADDEAKHLLEDCGRKCQEKICSIDPEKKKNIHFKLFKETGKPGEVVCKFAQDENAHLIIMGSRGLGTLRRTFLGSNSDYCVHHAHI 158          
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. Ensembl Nematostella
Match: EDO44805 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUT2])

HSP 1 Score: 91.6633 bits (226), Expect = 2.828e-22
Identity = 53/145 (36.55%), Postives = 83/145 (57.24%), Query Frame = 3
Query:  372 RSIMIPIDSSEHSKRAFRWYIKNMKQPNDLVLFIHVVEPVYATPAV---GMMEPPPIVLDDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIHVDTKPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHAHI 797
            R I+IP+D S+HS+RAF WY   + + ND V  +H  +P  A P     G   P     +D  + M+  +++ K + + +  + KD+ ++    +     PG  + + AKD  AD II+G+RG+GT+RRT +GSVS+  LHH HI
Sbjct:   10 RKIVIPVDGSKHSERAFDWYKGALHRGNDEVFVVHAFDPYAAPPTPYPYGFAFP-----EDWEQHMKKTVDDAKSVMEYYEKKCKDSKMKC-TMLTKPGDPGETICEIAKDKNADQIIMGSRGLGTVRRTIVGSVSEFCLHHTHI 148          
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. Planmine SMEST
Match: SMESG000016283.1 (SMESG000016283.1)

HSP 1 Score: 300.827 bits (769), Expect = 2.507e-103
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 3
Query:  363 MAKRSIMIPIDSSEHSKRAFRWYIKNMKQPNDLVLFIHVVEPVYATPAVGMMEPPPIVLDDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIHVDTKPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHAHIXXXXXXXEAK 827
            MAKRSIMIPIDSSEHSKRAFRWYIKNMKQPNDLVLFIHVVEPVYATPAVGMMEPPPIVLDDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIHVDTKPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHAHIPVVVVPPEAK
Sbjct:    1 MAKRSIMIPIDSSEHSKRAFRWYIKNMKQPNDLVLFIHVVEPVYATPAVGMMEPPPIVLDDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIHVDTKPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHAHIPVVVVPPEAK 155          
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. Planmine SMEST
Match: SMESG000022085.1 (SMESG000022085.1)

HSP 1 Score: 171.4 bits (433), Expect = 2.068e-52
Identity = 79/142 (55.63%), Postives = 106/142 (74.65%), Query Frame = 3
Query:  372 RSIMIPIDSSEHSKRAFRWYIKNMKQPNDLVLFIHVVEPVYATPAVGMMEPPPIVLDDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIHVDTKPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHAHI 797
            R +++P+D S++S+RAF WYI  M +  DL + I++VEPVYATP +   E   +  D +S+ M+D I +GK+L Q F+H AK+  +E KAFIHVD KPG ALVK AK+H+ADVI++GNRGV  L RTFLGSVSD+IL HAH+
Sbjct:    8 RVVLLPLDGSDNSERAFEWYIGQMHRRGDLAVLINIVEPVYATPPLNSAENSVLSNDSVSQQMQDIIEDGKQLTQRFLHMAKNANVEAKAFIHVDPKPGPALVKCAKEHRADVIVVGNRGVSKLHRTFLGSVSDYILQHAHL 149          
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. Planmine SMEST
Match: SMESG000039918.1 (SMESG000039918.1)

HSP 1 Score: 147.517 bits (371), Expect = 3.416e-43
Identity = 65/144 (45.14%), Postives = 102/144 (70.83%), Query Frame = 3
Query:  366 AKRSIMIPIDSSEHSKRAFRWYIKNMKQPNDLVLFIHVVEPVYATPAVGMMEPPPIVLDDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIHVDTKPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHAHI 797
            + R+I+IP+DSS+HS+RAF WY  N+KQ  D++  +H+VEP YAT A+ + +     ++DM+  M+ A  + K+L Q+++  A+D  +  K  I+VD K G A++K A +++  ++IIGNRG+GTLRRT LGS SD IL+HA++
Sbjct:    6 SNRNILIPVDSSDHSRRAFEWYQANIKQEGDVLFLVHIVEPQYATSAISISDTIKFKMEDMTSKMDSASKKAKELLQKYIKLAEDDRIHTKGIINVDNKAGDAIIKIASENRVSLVIIGNRGIGTLRRTILGSTSDFILNHANV 149          
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. Planmine SMEST
Match: SMESG000020952.1 (SMESG000020952.1)

HSP 1 Score: 145.591 bits (366), Expect = 1.738e-42
Identity = 65/143 (45.45%), Postives = 103/143 (72.03%), Query Frame = 3
Query:  366 AKRSIMIPIDSSEHSKRAFRWYIKNMKQPNDLVLFIHVVEPVYATPAVGM-MEPPPIVLDDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIHVDTKPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHA 791
             +R I++P+D SEH++RA  WY++N+ +PNDLV  IH+VEP+Y + AVG+ ME   + ++D+   ++  +++GK L ++F HE ++ G   K ++ VD+K G A++K    H+ D+II+ NRG G +RRTFLGSVSD++LHHA
Sbjct:    9 VERVILMPVDRSEHTERAIDWYVENLMKPNDLVQMIHIVEPMYTSTAVGIGMEVAFLPVEDLESKIKKQLDKGKSLVKKFTHELQEKGGRAKGYVIVDSKVGEAIIKVCHTHKVDLIIVANRGEGLIRRTFLGSVSDYVLHHA 151          
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. Planmine SMEST
Match: SMESG000020882.1 (SMESG000020882.1)

HSP 1 Score: 140.969 bits (354), Expect = 1.499e-40
Identity = 68/143 (47.55%), Postives = 98/143 (68.53%), Query Frame = 3
Query:  366 AKRSIMIPIDSSEHSKRAFRWYIKNMKQPNDLVLFIHVVEPVYATPAVGM-MEPPPIVLDDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIHVDTKPGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHA 791
            ++R I+IPID SE S+RA  WY  NM + ND+V  IH+VEP+Y T AVG+ ME   + ++++   ++  I++GK L +E++    D G + K FI VD++ G  ++    DH ADVI++ NRG G LRRTFLGSVSD++LHHA
Sbjct:   17 SERIIIIPIDKSEVSRRALDWYSNNMMKENDVVHLIHIVEPIYNTIAVGIGMEAAWLPMEELDAKIQKQIDKGKALVREYISNLTDKGGKCKGFIRVDSRVGECIISVCHDHLADVILMANRGEGLLRRTFLGSVSDYVLHHA 159          
The following BLAST results are available for this feature:
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|A0QZA1|Y3950_MYCS27.097e-829.50Universal stress protein MSMEG_3950/MSMEI_3859 OS=... [more]
sp|P74148|Y1388_SYNY37.325e-823.23Universal stress protein Sll1388 OS=Synechocystis ... [more]
sp|Q8LGG8|USPAL_ARATH8.917e-723.78Universal stress protein A-like protein OS=Arabido... [more]
sp|Q57951|Y531_METJA8.990e-728.47Universal stress protein MJ0531 OS=Methanocaldococ... [more]
back to top
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A448X5I92.773e-6567.83Usp domain-containing protein OS=Protopolystoma xe... [more]
A0A0R3U2V25.542e-6261.54Usp domain-containing protein OS=Mesocestoides cor... [more]
A0A183QRF31.845e-6163.45Usp domain-containing protein OS=Schistosoma rodha... [more]
A0A094ZGI84.248e-6163.45Universal stress protein OS=Schistosoma haematobiu... [more]
A0A183K3A84.683e-6163.45Usp domain-containing protein OS=Schistosoma curas... [more]
back to top
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO458002.705e-2937.32Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO447462.922e-2836.36Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO331431.332e-2434.46Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO381421.493e-2438.46Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO448052.828e-2236.55Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Universal stress protein MSMEG_3950/MSMEI_3859 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000016283.12.507e-103100.00SMESG000016283.1[more]
SMESG000022085.12.068e-5255.63SMESG000022085.1[more]
SMESG000039918.13.416e-4345.14SMESG000039918.1[more]
SMESG000020952.11.738e-4245.45SMESG000020952.1[more]
SMESG000020882.11.499e-4047.55SMESG000020882.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30025317 ID=SMED30025317|Name=Universal stress protein MSMEG_3950/MSMEI_3859|organism=Schmidtea mediterranea sexual|type=transcript|length=1062bp
CAAGGTGAACAGGAATGTTTTCATTAATTTGTGCTTTATTAATTATCTGA
AATAAATTACAAATATGCTGTTTATTGTAATCAGCAGTATGCGACAATAT
ATTTTATATGCGTGTATACGTAATCCGTATAAATGCGTAAACACACACAC
TCAATTCACCACAACGTGACTGTATTTTGACCTAGTTTACTTTCCGCTAA
TTGCATTTTGATTGGTTGATTCATTTCTATATAAATAGAATTTTGTAATC
AATTCTTTATAAATTTGGATTTATAGATTATAAATTTTCGCTATCTGATT
TTCGTTAGACAATAAATTCATTAATTTTCAAATTTCCTTTACTTAGTTTG
TTATTAATTCCAATGGCTAAAAGATCTATTATGATACCAATTGATAGTAG
CGAGCATAGCAAGAGAGCATTTCGATGGTACATAAAAAATATGAAACAGC
CAAACGATCTAGTGCTATTTATTCACGTAGTCGAACCTGTTTATGCTACT
CCAGCAGTTGGGATGATGGAGCCACCACCAATTGTATTAGACGACATGTC
ACGAATGATGGAAGATGCTATTAATGAAGGTAAGAAATTGGGGCAGGAAT
TCATGCATGAAGCGAAAGATACTGGATTAGAACCGAAAGCTTTTATTCAT
GTTGACACCAAGCCAGGAGCAGCATTAGTAAAATCAGCGAAAGATCATCA
AGCTGATGTTATTATAATAGGCAATAGAGGAGTTGGCACGTTACGTCGTA
CGTTTTTAGGCAGTGTTAGTGACCATATTCTACATCATGCTCACATTCCA
GTGGTTGTTGTTCCACCAGAAGCTAAATAATCAAATAGAATCAGAAAACT
TGCGATTTTTTTTGATTGTCTTGTCGTAAAATTCATATTAAAATAAATAT
TGTTTAATAATTTAAATTAAATTTTATTTCATAATGTGAGCTAAGTTTTT
TATTCATTGTATGCTATTATGTAATTATTTGACTAATAAACGATTTATTC
GCTTCCTAACTAATGTTTACAGTTGAATAGTTCCACTGAAATTTGTTGGC
ATTATTTAAACA
back to top

protein sequence of SMED30025317-orf-1

>SMED30025317-orf-1 ID=SMED30025317-orf-1|Name=SMED30025317-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=156bp
MAKRSIMIPIDSSEHSKRAFRWYIKNMKQPNDLVLFIHVVEPVYATPAVG
MMEPPPIVLDDMSRMMEDAINEGKKLGQEFMHEAKDTGLEPKAFIHVDTK
PGAALVKSAKDHQADVIIIGNRGVGTLRRTFLGSVSDHILHHAHIPVVVV
PPEAK*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0000166nucleotide binding
GO:0005524ATP binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000070intestinal phagocyte
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR006015Universal_stress_UspA
IPR014729Rossmann-like_a/b/a_fold
IPR006016UspA
Vocabulary: biological process
TermDefinition
GO:0006950response to stress
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006015Universal stress protein A familyPRINTSPR01438UNVRSLSTRESScoord: 129..151
score: 38.09
coord: 111..123
score: 34.77
coord: 3..21
score: 28.0
IPR006016UspAPFAMPF00582Uspcoord: 4..151
e-value: 2.4E-26
score: 92.9
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3DG3DSA:3.40.50.620coord: 2..155
e-value: 1.8E-36
score: 127.9
NoneNo IPR availablePANTHERPTHR46989FAMILY NOT NAMEDcoord: 4..153
NoneNo IPR availableCDDcd00293USP_Likecoord: 6..150
e-value: 1.58544E-28
score: 100.137
NoneNo IPR availableSUPERFAMILYSSF52402Adenine nucleotide alpha hydrolases-likecoord: 4..153