SMED30025195
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30025195 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Homology
BLAST of SMED30025195 vs. Ensembl Mouse
Match: Tmem237 (transmembrane protein 237 [Source:MGI Symbol;Acc:MGI:2138365]) HSP 1 Score: 51.2174 bits (121), Expect = 5.533e-6 Identity = 55/227 (24.23%), Postives = 96/227 (42.29%), Query Frame = 2 Query: 407 VSQGPGDKVYIEGVRNFRVENHDKYLKSQQKLSEKQAEKLRINENDYDNDLMQCSIGVTNRFRIFATFLHGILAGMTLMHLVFCLVVGGTTQSSQQSYLILYTTLQQPFSIFNYVAFVIILISLWDRVDVG------------QPSPNCSQPQIGVLVALILTFIAFIINNSLYLYNQDFINFNTNSTSAYLSTLVTSFGTWKILDAVKSILLVISWFIVAMVPIFD 1051 VSQ P KV++E R F+ + + +K+ + + K D ++ V FR+ F HG LAG + + V V+ G S+ + L Y L PF Y+ + +S +DR D +P+ S ALIL+ + ++ ++LY + +N + + A LV W I++ V ++L+ +SW ++ P D Sbjct: 194 VSQ-PVGKVFVEKSRRFQAADRSELIKTTENIDVSMDVKPSWTTRD-------VALSVHRAFRMVGLFSHGFLAGCAVWNTVVIYVLAGDQLSNVSNLLQQYKPLAYPFQSLLYLLLALSTVSAFDRTDFAKISVAIRNFLALEPTALAS---FLYFTALILSLSQQMTSDRIHLY-EPSVNGSLWAAEAEEPILV----PWIIVNLVVALLVGLSWLFLSYRPGMD 404
BLAST of SMED30025195 vs. Ensembl Mouse
Match: Tmem237 (transmembrane protein 237 [Source:MGI Symbol;Acc:MGI:2138365]) HSP 1 Score: 50.447 bits (119), Expect = 9.076e-6 Identity = 55/227 (24.23%), Postives = 96/227 (42.29%), Query Frame = 2 Query: 407 VSQGPGDKVYIEGVRNFRVENHDKYLKSQQKLSEKQAEKLRINENDYDNDLMQCSIGVTNRFRIFATFLHGILAGMTLMHLVFCLVVGGTTQSSQQSYLILYTTLQQPFSIFNYVAFVIILISLWDRVDVG------------QPSPNCSQPQIGVLVALILTFIAFIINNSLYLYNQDFINFNTNSTSAYLSTLVTSFGTWKILDAVKSILLVISWFIVAMVPIFD 1051 VSQ P KV++E R F+ + + +K+ + + K D ++ V FR+ F HG LAG + + V V+ G S+ + L Y L PF Y+ + +S +DR D +P+ S ALIL+ + ++ ++LY + +N + + A LV W I++ V ++L+ +SW ++ P D Sbjct: 170 VSQ-PVGKVFVEKSRRFQAADRSELIKTTENIDVSMDVKPSWTTRD-------VALSVHRAFRMVGLFSHGFLAGCAVWNTVVIYVLAGDQLSNVSNLLQQYKPLAYPFQSLLYLLLALSTVSAFDRTDFAKISVAIRNFLALEPTALAS---FLYFTALILSLSQQMTSDRIHLY-EPSVNGSLWAAEAEEPILV----PWIIVNLVVALLVGLSWLFLSYRPGMD 380
BLAST of SMED30025195 vs. TrEMBL
Match: A0A267GG12 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig002657g1 PE=4 SV=1) HSP 1 Score: 109.768 bits (273), Expect = 3.840e-21 Identity = 67/231 (29.00%), Postives = 119/231 (51.52%), Query Frame = 2 Query: 446 VRNFRVENHDKYLKSQQKLSEKQAEKLR----INENDYDNDLMQCSIGVTNRFRIFATFLHGILAGMTLMHLVFCLVVGGTTQSSQQSYLILYTTLQQPFSIFNYVAFVIILISLWDRVDVGQ----PSPNCSQPQIGVLVALILTFIAFIINNSLYLYNQDFINFNTNSTS----AYLSTLVTSFGTWKILDAVKSILLVISWFIVAMVPIFDRFIEQLNESINQELNEN 1102 + F+ E DKY QQ+L +++ E+ + + E D N ++C+IG T + T L G++ G L+H V+ T S+ S+L Y+ L P I YV +IL+S+ DR D G+ C+ + G +V ++L A++++N++Y +Q ++ N+T+ A +LV S W+I+D V++ L + W +VA P F+R + L ++ + E Sbjct: 240 AKTFKAEKLDKY---QQRLEQERKEREQYLEGVVEEDTSNRTLKCAIGFTKVYNYLGTVLLGLMPGFALLHTVYVW----TLSSNYLSFLADYSRLGSPMPILYYVVLTLILLSVLDRYDTGKLDRGSCLECASFRHGGMV-VLLYIPAYVVSNAMYPTDQWMRGYSLNATAYAGIANGDSLVQS---WRIMDTVRAACLALGWLVVAFNPQFNRLADNLETELDDLIGEE 459
BLAST of SMED30025195 vs. TrEMBL
Match: A0A1I8GPS1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 109.768 bits (273), Expect = 4.161e-21 Identity = 67/231 (29.00%), Postives = 119/231 (51.52%), Query Frame = 2 Query: 446 VRNFRVENHDKYLKSQQKLSEKQAEKLR----INENDYDNDLMQCSIGVTNRFRIFATFLHGILAGMTLMHLVFCLVVGGTTQSSQQSYLILYTTLQQPFSIFNYVAFVIILISLWDRVDVGQ----PSPNCSQPQIGVLVALILTFIAFIINNSLYLYNQDFINFNTNSTS----AYLSTLVTSFGTWKILDAVKSILLVISWFIVAMVPIFDRFIEQLNESINQELNEN 1102 + F+ E DKY QQ+L +++ E+ + + E D N ++C+IG T + T L G++ G L+H V+ T S+ S+L Y+ L P I YV +IL+S+ DR D G+ C+ + G +V ++L A++++N++Y +Q ++ N+T+ A +LV S W+I+D V++ L + W +VA P F+R + L ++ + E Sbjct: 240 AKTFKAEKLDKY---QQRLEQERKEREQYLEGVVEEDTSNRTLKCAIGFTKVYNYLGTVLLGLMPGFALLHTVYVW----TLSSNYLSFLADYSRLGSPMPILYYVVLTLILLSVLDRYDTGKLDRGSCLECASFRHGGMV-VLLYIPAYVVSNAMYPTDQWMRGYSLNATAYAGIANGDSLVQS---WRIMDTVRAACLALGWLVVAFNPQFNRLADNLETELDDLIGEE 459
BLAST of SMED30025195 vs. TrEMBL
Match: A0A1I8I513 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 107.071 bits (266), Expect = 1.275e-19 Identity = 67/223 (30.04%), Postives = 116/223 (52.02%), Query Frame = 2 Query: 446 VRNFRVENHDKYLKSQQKLSEKQAEKLR----INENDYDNDLMQCSIGVTNRFRIFATFLHGILAGMTLMHLVFCLVVGGTTQSSQQSYLILYTTLQQPFSIFNYVAFVIILISLWDRVDVGQ----PSPNCSQPQIGVLVALILTFIAFIINNSLYLYNQDFINFNTNSTS----AYLSTLVTSFGTWKILDAVKSILLVISWFIVAMVPIFDRFIEQLNES 1078 + F+ E DKY QQ+L +++ E+ + + E D N ++C+IG T + T L G++ G L+H V+ T S+ S+L Y+ L P I YV +IL+S+ DR D G+ C+ + G +V ++L A++++N++Y +Q ++ N+T+ A +LV S W+I+D V++ L + W +VA P F+R + L S Sbjct: 1008 AKTFKAEKLDKY---QQRLEQERKEREQYLEGVVEEDTSNRTLKCAIGFTKVYNYLGTVLLGLMPGFALLHTVYVW----TLSSNYLSFLADYSRLGSPMPILYYVVLTLILLSVLDRYDTGKLDRGSCLECASFRHGGMV-VLLYIPAYVVSNAMYPTDQWMRGYSLNATAYAGIANGDSLVQS---WRIMDTVRAACLALGWLVVAFNPQFNRLADNLETS 1219
BLAST of SMED30025195 vs. TrEMBL
Match: A0A1I8GZE5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 107.071 bits (266), Expect = 1.890e-19 Identity = 68/232 (29.31%), Postives = 119/232 (51.29%), Query Frame = 2 Query: 440 EGVRNFRVENHDKYLKSQQKLSEKQAEKLR----INENDYDNDLMQCSIGVTNRFRIFATFLHGILAGMTLMHLVFCLVVGGTTQSSQQSYLILYTTLQQPFSIFNYVAFVIILISLWDRVDVGQ----PSPNCSQPQIGVLVALILTFIAFIINNSLYLYNQDFINFNTNSTS----AYLSTLVTSFGTWKILDAVKSILLVISWFIVAMVPIFDRFIEQLNESINQELNE 1099 + + F+ E DKY QQ+L +++ E+ + + E D N ++C+IG T + T L G++ G L+H V+ T S+ S+L Y+ L P I YV +IL+S+ DR D G+ C+ + G +V L L A++++N++Y +Q ++ N+T+ A +LV S W+I+D V++ L + W +VA P F+R + L ++ + E Sbjct: 1418 QDAKTFKAEKLDKY---QQRLEQERKEREQYLEGVVEEDTSNRTLKCAIGFTKVYNYLGTVLLGLMPGFALLHTVYVW----TLSSNYLSFLADYSRLGSPMPILYYVVLTLILLSVLDRYDTGKLDRGSCLECASFRHGGMVVL-LYIPAYVVSNAMYPTDQWMRGYSLNATAYAGIANGDSLVQS---WRIMDTVRAACLALGWLVVAFNPQFNRLADNLETELDDLIGE 1638
BLAST of SMED30025195 vs. TrEMBL
Match: A0A183VY35 (Uncharacterized protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS5391 PE=4 SV=1) HSP 1 Score: 88.9669 bits (219), Expect = 3.231e-15 Identity = 80/286 (27.97%), Postives = 138/286 (48.25%), Query Frame = 2 Query: 287 NNGPLGVGAYLEDLQDDIEMKVNSDDLNNEILTISRGKPSVSQGPGDKVYIEGVRNFRVENHDKYLK----SQQKLS---EKQAEKLRINENDYDNDLMQCSIGVTNRFRIFATFLHGILAGMTLMHLVFCLVVGGTTQ---SSQQSYLIL--YTTLQQPFSIFNYVAFVIILISLWDRVDVGQPSPNCSQPQIGV---LVALILTFIAFIINNSLYLYNQ--DFINFNTNSTSAYL----STLVTSFGTWKILDAVKSILLVISWFIVAMV-PIFDRFIEQLNES 1078 NNG +G L+ L DDI + + +NN R + G R V N Y K QQK S K +K R+ N +N + + + ++ + L G+LAG +L+H +F + Q S+ Q YL+L Y +P SI Y + ++ + ++ + D+G+P+ NC Q + + L+A+I + FIINNS+ ++ ++ +++ YL S L +S W ILDA ++ L++I+W V + P++DR ++ L+ S Sbjct: 29 NNGD--IGPILDALGDDITIASEMETVNNR-----RNSQAT-----------GARKIPVINSHDYEKNISTKQQKESIAKLKSQDKQRL-INMSNNPGLYFVVKIHETLQMLSFTLFGVLAGASLVHTLFVQALANAIQIGSSTNQYYLLLRLYGAYARPVSITFYASLAVVGVFIFGKFDLGRPTINCIQKCVRLQNGLMAIIFYMLCFIINNSMSWFDNVLHTTSYKSDTQINYLLNTDSELNSSLKIWLILDAGRTALILITWISVGLGDPLYDRLVKTLSSS 295
BLAST of SMED30025195 vs. Ensembl Cavefish
Match: tmem237b (transmembrane protein 237B [Source:NCBI gene;Acc:103023480]) HSP 1 Score: 56.225 bits (134), Expect = 1.031e-7 Identity = 67/266 (25.19%), Postives = 112/266 (42.11%), Query Frame = 2 Query: 323 DLQDDIEMKVNSDDLNNEILTISRGKPSVSQG-----------PGDKVYIEGVRNFR-VENHDKYLKSQQKLSEKQAEKLRINENDYDNDLMQCSIGVTNR----FRIFATFLHGILAGMTLMHLVFCLVVGGTTQSSQQSYLILYTTLQQPFSIFNYVAFVIILISLWDRVDVGQPSPNCSQ-----PQIGVLVALILTFIAFIINNSLYLYNQDFINF--NTNSTSAYLSTLVTSFGTWKILDAVKSILLVISWFIVAMVPIFD 1051 D+Q + E+ V DD+ I+ +P + Q P KV+IE R F+ E D S Q +N D M + V+ R FR+ F HG LAG + +++ ++ G S+ + L Y TL P Y + +S +DRV++ + S P V +A L F A +++ S + D IN N N++ + +S W I++ V ++L+ ++W +V+ P D Sbjct: 144 DMQLNNELAVEDDDI------ITDAQPPIPQHALFSAPHGQSQPVAKVFIERGRRFQPAERIDMRKTSDQP-----------EQNFMDLHSMWTTRDVSMRVHRGFRVIGLFSHGFLAGYAVWNIIVMYILAGEQMSTLPNLLQQYHTLAYPAQSLFYFLLALSTVSAFDRVNLAKASVALRSFLTLDP---VALASFLYFSALVLSLSQQM-TSDRINLYSNINASLWLPESEHSSLYPWIIVNLVVTLLVGLAWVLVSTSPDVD 388
BLAST of SMED30025195 vs. Ensembl Medaka
Match: tmem237b (transmembrane protein 237B [Source:NCBI gene;Acc:101166111]) HSP 1 Score: 55.8398 bits (133), Expect = 1.471e-7 Identity = 61/253 (24.11%), Postives = 111/253 (43.87%), Query Frame = 2 Query: 323 DLQDDIEMKVNSDDLNNEILTISRGKPSVSQGPGD---KVYIEGVRNFRVENHDKYLKSQQKLSEKQAEKLRINENDYDNDLMQCSIGVTNRFRIFATFLHGILAGMTLMHLVFCLVVGGTTQSSQQSYLILYTTLQQPFSIFNYVAFVIILISLWDRVDVGQPSPNCSQ-----PQIGVLVALILTFIAFIINNSLYLYNQDFINF--NTNSTSAYLSTLVTSFGTWKILDAVKSILLVISWFIVAMVPIFD 1051 DL DD++ + DD+ + S QG KV++E R F+ KS Q++ + A+ + + D+ S+ V FR+F + HG LAG + ++V ++ G ++ + L Y +L P Y+ I ++ +DRV++ + + + P V +A L F A +++ S + D IN + N+T + W ++ V ++L+ I+W VA P D Sbjct: 126 DLPDDLD--IQEDDVVTDYPIPQHALFSAPQGQSQAVAKVFVERNRRFQAAERADLRKSSQQM-DHTADFQHLQQPWTTRDV---SVRVHGGFRVFGLYCHGFLAGYAVWNVVVVYMLAGQHLTALANLLEQYHSLAYPSQSLLYMLLAISTVAAFDRVNLAKGTVALREFITLDP---VALASFLYFSALVLSLSQQM-TSDRINLYPSYNATLWPPGSEHQILHPWVTVNLVVALLVGIAWICVATRPEAD 368
BLAST of SMED30025195 vs. Ensembl Medaka
Match: tmem237b (transmembrane protein 237B [Source:NCBI gene;Acc:101166111]) HSP 1 Score: 55.4546 bits (132), Expect = 1.755e-7 Identity = 61/253 (24.11%), Postives = 111/253 (43.87%), Query Frame = 2 Query: 323 DLQDDIEMKVNSDDLNNEILTISRGKPSVSQGPGD---KVYIEGVRNFRVENHDKYLKSQQKLSEKQAEKLRINENDYDNDLMQCSIGVTNRFRIFATFLHGILAGMTLMHLVFCLVVGGTTQSSQQSYLILYTTLQQPFSIFNYVAFVIILISLWDRVDVGQPSPNCSQ-----PQIGVLVALILTFIAFIINNSLYLYNQDFINF--NTNSTSAYLSTLVTSFGTWKILDAVKSILLVISWFIVAMVPIFD 1051 DL DD++ + DD+ + S QG KV++E R F+ KS Q++ + A+ + + D+ S+ V FR+F + HG LAG + ++V ++ G ++ + L Y +L P Y+ I ++ +DRV++ + + + P V +A L F A +++ S + D IN + N+T + W ++ V ++L+ I+W VA P D Sbjct: 138 DLPDDLD--IQEDDVVTDYPIPQHALFSAPQGQSQAVAKVFVERNRRFQAAERADLRKSSQQM-DHTADFQHLQQPWTTRDV---SVRVHGGFRVFGLYCHGFLAGYAVWNVVVVYMLAGQHLTALANLLEQYHSLAYPSQSLLYMLLAISTVAAFDRVNLAKGTVALREFITLDP---VALASFLYFSALVLSLSQQM-TSDRINLYPSYNATLWPPGSEHQILHPWVTVNLVVALLVGIAWICVATRPEAD 380
BLAST of SMED30025195 vs. Planmine SMEST
Match: SMESG000001693.1 (SMESG000001693.1) HSP 1 Score: 159.458 bits (402), Expect = 3.885e-45 Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 2 Query: 80 MSENVHDNPVYADENANNGXXXXXXXXXXXXXXXXXXXXXXXXXDNSNDVXXXXXXXXXXXXXQSEDVNNNGPLGVGAYLEDLQDDIEMKVNSDDLNNEILTISRGKPSVSQGPGDKVYIEG 445 MSENVHDNPVYADENANNGVRKKRRRRPNPNETETTNTVPEPNNDNSNDVKREPRRRRRKERRQSEDVNNNGPLGVGAYLEDLQDDIEMKVNSDDLNNEILTISRGKPSVSQGPGDKVYIEG Sbjct: 1 MSENVHDNPVYADENANNGVRKKRRRRPNPNETETTNTVPEPNNDNSNDVKREPRRRRRKERRQSEDVNNNGPLGVGAYLEDLQDDIEMKVNSDDLNNEILTISRGKPSVSQGPGDKVYIEG 122 The following BLAST results are available for this feature:
BLAST of SMED30025195 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30025195 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30025195 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30025195 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30025195 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30025195 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 2
BLAST of SMED30025195 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30025195 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30025195 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 1
BLAST of SMED30025195 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30025195 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30025195 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30025195 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 2
BLAST of SMED30025195 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30025195 ID=SMED30025195|Name=SMED30025195|organism=Schmidtea mediterranea sexual|type=transcript|length=3067bpback to top protein sequence of SMED30025195-orf-1 >SMED30025195-orf-1 ID=SMED30025195-orf-1|Name=SMED30025195-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=343bp MSENVHDNPVYADENANNGVRKKRRRRPNPNETETTNTVPEPNNDNSNDVback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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