SMED30025195

Overview


Name	SMED30025195
Smed ID	SMED30025195
Length (bp)	3067

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30025195 (SMED30025195) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30025195 (SMED30025195) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30025195 (SMED30025195) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30025195 (SMED30025195) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30025195

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 6

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
head region	SMED30025195	h1SMcG0018008	SmedASXL_009103	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
pharynx	SMED30025195	h1SMcG0018008	dd_Smed_v4_10480_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30025195	h1SMcG0018008	dd_Smed_v4_10480_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchyma	SMED30025195	h1SMcG0018008	dd_Smed_v4_10480_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
head region	SMED30025195	h1SMcG0018008	dd_Smed_v6_10480_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
epidermis	SMED30025195	h1SMcG0018008	dd_Smed_v4_10480_0_1	dd_Smed_v4	PMID:28292427 Wurtzel et al., 2017

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of SMED30025195 vs. Ensembl Mouse
Match: Tmem237 (transmembrane protein 237 [Source:MGI Symbol;Acc:MGI:2138365])

HSP 1 Score: 51.2174 bits (121), Expect = 5.533e-6
Identity = 55/227 (24.23%), Postives = 96/227 (42.29%), Query Frame = 2

Query:  407 VSQGPGDKVYIEGVRNFRVENHDKYLKSQQKLSEKQAEKLRINENDYDNDLMQCSIGVTNRFRIFATFLHGILAGMTLMHLVFCLVVGGTTQSSQQSYLILYTTLQQPFSIFNYVAFVIILISLWDRVDVG------------QPSPNCSQPQIGVLVALILTFIAFIINNSLYLYNQDFINFNTNSTSAYLSTLVTSFGTWKILDAVKSILLVISWFIVAMVPIFD 1051
            VSQ P  KV++E  R F+  +  + +K+ + +      K      D        ++ V   FR+   F HG LAG  + + V   V+ G   S+  + L  Y  L  PF    Y+   +  +S +DR D              +P+   S        ALIL+    + ++ ++LY +  +N +  +  A    LV     W I++ V ++L+ +SW  ++  P  D
Sbjct:  194 VSQ-PVGKVFVEKSRRFQAADRSELIKTTENIDVSMDVKPSWTTRD-------VALSVHRAFRMVGLFSHGFLAGCAVWNTVVIYVLAGDQLSNVSNLLQQYKPLAYPFQSLLYLLLALSTVSAFDRTDFAKISVAIRNFLALEPTALAS---FLYFTALILSLSQQMTSDRIHLY-EPSVNGSLWAAEAEEPILV----PWIIVNLVVALLVGLSWLFLSYRPGMD 404

[Close]

BLAST of SMED30025195 vs. Ensembl Mouse
Match: Tmem237 (transmembrane protein 237 [Source:MGI Symbol;Acc:MGI:2138365])

HSP 1 Score: 50.447 bits (119), Expect = 9.076e-6
Identity = 55/227 (24.23%), Postives = 96/227 (42.29%), Query Frame = 2

Query:  407 VSQGPGDKVYIEGVRNFRVENHDKYLKSQQKLSEKQAEKLRINENDYDNDLMQCSIGVTNRFRIFATFLHGILAGMTLMHLVFCLVVGGTTQSSQQSYLILYTTLQQPFSIFNYVAFVIILISLWDRVDVG------------QPSPNCSQPQIGVLVALILTFIAFIINNSLYLYNQDFINFNTNSTSAYLSTLVTSFGTWKILDAVKSILLVISWFIVAMVPIFD 1051
            VSQ P  KV++E  R F+  +  + +K+ + +      K      D        ++ V   FR+   F HG LAG  + + V   V+ G   S+  + L  Y  L  PF    Y+   +  +S +DR D              +P+   S        ALIL+    + ++ ++LY +  +N +  +  A    LV     W I++ V ++L+ +SW  ++  P  D
Sbjct:  170 VSQ-PVGKVFVEKSRRFQAADRSELIKTTENIDVSMDVKPSWTTRD-------VALSVHRAFRMVGLFSHGFLAGCAVWNTVVIYVLAGDQLSNVSNLLQQYKPLAYPFQSLLYLLLALSTVSAFDRTDFAKISVAIRNFLALEPTALAS---FLYFTALILSLSQQMTSDRIHLY-EPSVNGSLWAAEAEEPILV----PWIIVNLVVALLVGLSWLFLSYRPGMD 380

[Close]

BLAST of SMED30025195 vs. TrEMBL
Match: A0A267GG12 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig002657g1 PE=4 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 3.840e-21
Identity = 67/231 (29.00%), Postives = 119/231 (51.52%), Query Frame = 2

Query:  446 VRNFRVENHDKYLKSQQKLSEKQAEKLR----INENDYDNDLMQCSIGVTNRFRIFATFLHGILAGMTLMHLVFCLVVGGTTQSSQQSYLILYTTLQQPFSIFNYVAFVIILISLWDRVDVGQ----PSPNCSQPQIGVLVALILTFIAFIINNSLYLYNQDFINFNTNSTS----AYLSTLVTSFGTWKILDAVKSILLVISWFIVAMVPIFDRFIEQLNESINQELNEN 1102
             + F+ E  DKY   QQ+L +++ E+ +    + E D  N  ++C+IG T  +    T L G++ G  L+H V+      T  S+  S+L  Y+ L  P  I  YV   +IL+S+ DR D G+        C+  + G +V ++L   A++++N++Y  +Q    ++ N+T+    A   +LV S   W+I+D V++  L + W +VA  P F+R  + L   ++  + E 
Sbjct:  240 AKTFKAEKLDKY---QQRLEQERKEREQYLEGVVEEDTSNRTLKCAIGFTKVYNYLGTVLLGLMPGFALLHTVYVW----TLSSNYLSFLADYSRLGSPMPILYYVVLTLILLSVLDRYDTGKLDRGSCLECASFRHGGMV-VLLYIPAYVVSNAMYPTDQWMRGYSLNATAYAGIANGDSLVQS---WRIMDTVRAACLALGWLVVAFNPQFNRLADNLETELDDLIGEE 459

[Close]

BLAST of SMED30025195 vs. TrEMBL
Match: A0A1I8GPS1 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 109.768 bits (273), Expect = 4.161e-21
Identity = 67/231 (29.00%), Postives = 119/231 (51.52%), Query Frame = 2

Query:  446 VRNFRVENHDKYLKSQQKLSEKQAEKLR----INENDYDNDLMQCSIGVTNRFRIFATFLHGILAGMTLMHLVFCLVVGGTTQSSQQSYLILYTTLQQPFSIFNYVAFVIILISLWDRVDVGQ----PSPNCSQPQIGVLVALILTFIAFIINNSLYLYNQDFINFNTNSTS----AYLSTLVTSFGTWKILDAVKSILLVISWFIVAMVPIFDRFIEQLNESINQELNEN 1102
             + F+ E  DKY   QQ+L +++ E+ +    + E D  N  ++C+IG T  +    T L G++ G  L+H V+      T  S+  S+L  Y+ L  P  I  YV   +IL+S+ DR D G+        C+  + G +V ++L   A++++N++Y  +Q    ++ N+T+    A   +LV S   W+I+D V++  L + W +VA  P F+R  + L   ++  + E 
Sbjct:  240 AKTFKAEKLDKY---QQRLEQERKEREQYLEGVVEEDTSNRTLKCAIGFTKVYNYLGTVLLGLMPGFALLHTVYVW----TLSSNYLSFLADYSRLGSPMPILYYVVLTLILLSVLDRYDTGKLDRGSCLECASFRHGGMV-VLLYIPAYVVSNAMYPTDQWMRGYSLNATAYAGIANGDSLVQS---WRIMDTVRAACLALGWLVVAFNPQFNRLADNLETELDDLIGEE 459

[Close]

BLAST of SMED30025195 vs. TrEMBL
Match: A0A1I8I513 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 1.275e-19
Identity = 67/223 (30.04%), Postives = 116/223 (52.02%), Query Frame = 2

Query:  446 VRNFRVENHDKYLKSQQKLSEKQAEKLR----INENDYDNDLMQCSIGVTNRFRIFATFLHGILAGMTLMHLVFCLVVGGTTQSSQQSYLILYTTLQQPFSIFNYVAFVIILISLWDRVDVGQ----PSPNCSQPQIGVLVALILTFIAFIINNSLYLYNQDFINFNTNSTS----AYLSTLVTSFGTWKILDAVKSILLVISWFIVAMVPIFDRFIEQLNES 1078
             + F+ E  DKY   QQ+L +++ E+ +    + E D  N  ++C+IG T  +    T L G++ G  L+H V+      T  S+  S+L  Y+ L  P  I  YV   +IL+S+ DR D G+        C+  + G +V ++L   A++++N++Y  +Q    ++ N+T+    A   +LV S   W+I+D V++  L + W +VA  P F+R  + L  S
Sbjct: 1008 AKTFKAEKLDKY---QQRLEQERKEREQYLEGVVEEDTSNRTLKCAIGFTKVYNYLGTVLLGLMPGFALLHTVYVW----TLSSNYLSFLADYSRLGSPMPILYYVVLTLILLSVLDRYDTGKLDRGSCLECASFRHGGMV-VLLYIPAYVVSNAMYPTDQWMRGYSLNATAYAGIANGDSLVQS---WRIMDTVRAACLALGWLVVAFNPQFNRLADNLETS 1219

[Close]

BLAST of SMED30025195 vs. TrEMBL
Match: A0A1I8GZE5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 1.890e-19
Identity = 68/232 (29.31%), Postives = 119/232 (51.29%), Query Frame = 2

Query:  440 EGVRNFRVENHDKYLKSQQKLSEKQAEKLR----INENDYDNDLMQCSIGVTNRFRIFATFLHGILAGMTLMHLVFCLVVGGTTQSSQQSYLILYTTLQQPFSIFNYVAFVIILISLWDRVDVGQ----PSPNCSQPQIGVLVALILTFIAFIINNSLYLYNQDFINFNTNSTS----AYLSTLVTSFGTWKILDAVKSILLVISWFIVAMVPIFDRFIEQLNESINQELNE 1099
            +  + F+ E  DKY   QQ+L +++ E+ +    + E D  N  ++C+IG T  +    T L G++ G  L+H V+      T  S+  S+L  Y+ L  P  I  YV   +IL+S+ DR D G+        C+  + G +V L L   A++++N++Y  +Q    ++ N+T+    A   +LV S   W+I+D V++  L + W +VA  P F+R  + L   ++  + E
Sbjct: 1418 QDAKTFKAEKLDKY---QQRLEQERKEREQYLEGVVEEDTSNRTLKCAIGFTKVYNYLGTVLLGLMPGFALLHTVYVW----TLSSNYLSFLADYSRLGSPMPILYYVVLTLILLSVLDRYDTGKLDRGSCLECASFRHGGMVVL-LYIPAYVVSNAMYPTDQWMRGYSLNATAYAGIANGDSLVQS---WRIMDTVRAACLALGWLVVAFNPQFNRLADNLETELDDLIGE 1638

[Close]

BLAST of SMED30025195 vs. TrEMBL
Match: A0A183VY35 (Uncharacterized protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS5391 PE=4 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 3.231e-15
Identity = 80/286 (27.97%), Postives = 138/286 (48.25%), Query Frame = 2

Query:  287 NNGPLGVGAYLEDLQDDIEMKVNSDDLNNEILTISRGKPSVSQGPGDKVYIEGVRNFRVENHDKYLK----SQQKLS---EKQAEKLRINENDYDNDLMQCSIGVTNRFRIFATFLHGILAGMTLMHLVFCLVVGGTTQ---SSQQSYLIL--YTTLQQPFSIFNYVAFVIILISLWDRVDVGQPSPNCSQPQIGV---LVALILTFIAFIINNSLYLYNQ--DFINFNTNSTSAYL----STLVTSFGTWKILDAVKSILLVISWFIVAMV-PIFDRFIEQLNES 1078
            NNG   +G  L+ L DDI +    + +NN      R   +            G R   V N   Y K     QQK S    K  +K R+  N  +N  +   + +    ++ +  L G+LAG +L+H +F   +    Q   S+ Q YL+L  Y    +P SI  Y +  ++ + ++ + D+G+P+ NC Q  + +   L+A+I   + FIINNS+  ++      ++ +++   YL    S L +S   W ILDA ++ L++I+W  V +  P++DR ++ L+ S
Sbjct:   29 NNGD--IGPILDALGDDITIASEMETVNNR-----RNSQAT-----------GARKIPVINSHDYEKNISTKQQKESIAKLKSQDKQRL-INMSNNPGLYFVVKIHETLQMLSFTLFGVLAGASLVHTLFVQALANAIQIGSSTNQYYLLLRLYGAYARPVSITFYASLAVVGVFIFGKFDLGRPTINCIQKCVRLQNGLMAIIFYMLCFIINNSMSWFDNVLHTTSYKSDTQINYLLNTDSELNSSLKIWLILDAGRTALILITWISVGLGDPLYDRLVKTLSSS 295

[Close]

BLAST of SMED30025195 vs. Ensembl Cavefish
Match: tmem237b (transmembrane protein 237B [Source:NCBI gene;Acc:103023480])

HSP 1 Score: 56.225 bits (134), Expect = 1.031e-7
Identity = 67/266 (25.19%), Postives = 112/266 (42.11%), Query Frame = 2

Query:  323 DLQDDIEMKVNSDDLNNEILTISRGKPSVSQG-----------PGDKVYIEGVRNFR-VENHDKYLKSQQKLSEKQAEKLRINENDYDNDLMQCSIGVTNR----FRIFATFLHGILAGMTLMHLVFCLVVGGTTQSSQQSYLILYTTLQQPFSIFNYVAFVIILISLWDRVDVGQPSPNCSQ-----PQIGVLVALILTFIAFIINNSLYLYNQDFINF--NTNSTSAYLSTLVTSFGTWKILDAVKSILLVISWFIVAMVPIFD 1051
            D+Q + E+ V  DD+      I+  +P + Q            P  KV+IE  R F+  E  D    S Q             +N  D   M  +  V+ R    FR+   F HG LAG  + +++   ++ G   S+  + L  Y TL  P     Y    +  +S +DRV++ + S          P   V +A  L F A +++ S  +   D IN   N N++     +  +S   W I++ V ++L+ ++W +V+  P  D
Sbjct:  144 DMQLNNELAVEDDDI------ITDAQPPIPQHALFSAPHGQSQPVAKVFIERGRRFQPAERIDMRKTSDQP-----------EQNFMDLHSMWTTRDVSMRVHRGFRVIGLFSHGFLAGYAVWNIIVMYILAGEQMSTLPNLLQQYHTLAYPAQSLFYFLLALSTVSAFDRVNLAKASVALRSFLTLDP---VALASFLYFSALVLSLSQQM-TSDRINLYSNINASLWLPESEHSSLYPWIIVNLVVTLLVGLAWVLVSTSPDVD 388

[Close]

BLAST of SMED30025195 vs. Ensembl Medaka
Match: tmem237b (transmembrane protein 237B [Source:NCBI gene;Acc:101166111])

HSP 1 Score: 55.8398 bits (133), Expect = 1.471e-7
Identity = 61/253 (24.11%), Postives = 111/253 (43.87%), Query Frame = 2

Query:  323 DLQDDIEMKVNSDDLNNEILTISRGKPSVSQGPGD---KVYIEGVRNFRVENHDKYLKSQQKLSEKQAEKLRINENDYDNDLMQCSIGVTNRFRIFATFLHGILAGMTLMHLVFCLVVGGTTQSSQQSYLILYTTLQQPFSIFNYVAFVIILISLWDRVDVGQPSPNCSQ-----PQIGVLVALILTFIAFIINNSLYLYNQDFINF--NTNSTSAYLSTLVTSFGTWKILDAVKSILLVISWFIVAMVPIFD 1051
            DL DD++  +  DD+  +         S  QG      KV++E  R F+        KS Q++ +  A+   + +     D+   S+ V   FR+F  + HG LAG  + ++V   ++ G   ++  + L  Y +L  P     Y+   I  ++ +DRV++ + +    +     P   V +A  L F A +++ S  +   D IN   + N+T     +       W  ++ V ++L+ I+W  VA  P  D
Sbjct:  126 DLPDDLD--IQEDDVVTDYPIPQHALFSAPQGQSQAVAKVFVERNRRFQAAERADLRKSSQQM-DHTADFQHLQQPWTTRDV---SVRVHGGFRVFGLYCHGFLAGYAVWNVVVVYMLAGQHLTALANLLEQYHSLAYPSQSLLYMLLAISTVAAFDRVNLAKGTVALREFITLDP---VALASFLYFSALVLSLSQQM-TSDRINLYPSYNATLWPPGSEHQILHPWVTVNLVVALLVGIAWICVATRPEAD 368

[Close]

BLAST of SMED30025195 vs. Ensembl Medaka
Match: tmem237b (transmembrane protein 237B [Source:NCBI gene;Acc:101166111])

HSP 1 Score: 55.4546 bits (132), Expect = 1.755e-7
Identity = 61/253 (24.11%), Postives = 111/253 (43.87%), Query Frame = 2

Query:  323 DLQDDIEMKVNSDDLNNEILTISRGKPSVSQGPGD---KVYIEGVRNFRVENHDKYLKSQQKLSEKQAEKLRINENDYDNDLMQCSIGVTNRFRIFATFLHGILAGMTLMHLVFCLVVGGTTQSSQQSYLILYTTLQQPFSIFNYVAFVIILISLWDRVDVGQPSPNCSQ-----PQIGVLVALILTFIAFIINNSLYLYNQDFINF--NTNSTSAYLSTLVTSFGTWKILDAVKSILLVISWFIVAMVPIFD 1051
            DL DD++  +  DD+  +         S  QG      KV++E  R F+        KS Q++ +  A+   + +     D+   S+ V   FR+F  + HG LAG  + ++V   ++ G   ++  + L  Y +L  P     Y+   I  ++ +DRV++ + +    +     P   V +A  L F A +++ S  +   D IN   + N+T     +       W  ++ V ++L+ I+W  VA  P  D
Sbjct:  138 DLPDDLD--IQEDDVVTDYPIPQHALFSAPQGQSQAVAKVFVERNRRFQAAERADLRKSSQQM-DHTADFQHLQQPWTTRDV---SVRVHGGFRVFGLYCHGFLAGYAVWNVVVVYMLAGQHLTALANLLEQYHSLAYPSQSLLYMLLAISTVAAFDRVNLAKGTVALREFITLDP---VALASFLYFSALVLSLSQQM-TSDRINLYPSYNATLWPPGSEHQILHPWVTVNLVVALLVGIAWICVATRPEAD 380

[Close]

BLAST of SMED30025195 vs. Planmine SMEST
Match: SMESG000001693.1 (SMESG000001693.1)

HSP 1 Score: 159.458 bits (402), Expect = 3.885e-45
Identity = 122/122 (100.00%), Postives = 122/122 (100.00%), Query Frame = 2

Query:   80 MSENVHDNPVYADENANNGXXXXXXXXXXXXXXXXXXXXXXXXXDNSNDVXXXXXXXXXXXXXQSEDVNNNGPLGVGAYLEDLQDDIEMKVNSDDLNNEILTISRGKPSVSQGPGDKVYIEG 445
            MSENVHDNPVYADENANNGVRKKRRRRPNPNETETTNTVPEPNNDNSNDVKREPRRRRRKERRQSEDVNNNGPLGVGAYLEDLQDDIEMKVNSDDLNNEILTISRGKPSVSQGPGDKVYIEG
Sbjct:    1 MSENVHDNPVYADENANNGVRKKRRRRPNPNETETTNTVPEPNNDNSNDVKREPRRRRRKERRQSEDVNNNGPLGVGAYLEDLQDDIEMKVNSDDLNNEILTISRGKPSVSQGPGDKVYIEG 122

The following BLAST results are available for this feature:

BLAST of SMED30025195 vs. Ensembl Human
BLAST of SMED30025195 vs. Ensembl Celegans
BLAST of SMED30025195 vs. Ensembl Fly
BLAST of SMED30025195 vs. Ensembl Zebrafish
BLAST of SMED30025195 vs. Ensembl Xenopus
BLAST of SMED30025195 vs. Ensembl Mouse
BLAST of SMED30025195 vs. UniProt/SwissProt
BLAST of SMED30025195 vs. TrEMBL
BLAST of SMED30025195 vs. Ensembl Cavefish
BLAST of SMED30025195 vs. Ensembl Sea Lamprey
BLAST of SMED30025195 vs. Ensembl Yeast
BLAST of SMED30025195 vs. Ensembl Nematostella
BLAST of SMED30025195 vs. Ensembl Medaka
BLAST of SMED30025195 vs. Planmine SMEST

BLAST of SMED30025195 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30025195 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30025195 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30025195 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30025195 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30025195 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2


Match Name	E-value	Identity	Description
Tmem237	5.533e-6	24.23	transmembrane protein 237 [Source:MGI Symbol;Acc:M...	[more]
Tmem237	9.076e-6	24.23	transmembrane protein 237 [Source:MGI Symbol;Acc:M...	[more]

BLAST of SMED30025195 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30025195 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A267GG12	3.840e-21	29.00	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]
A0A1I8GPS1	4.161e-21	29.00	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]
A0A1I8I513	1.275e-19	30.04	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]
A0A1I8GZE5	1.890e-19	29.31	Uncharacterized protein OS=Macrostomum lignano OX=...	[more]
A0A183VY35	3.231e-15	27.97	Uncharacterized protein OS=Trichobilharzia regenti...	[more]

BLAST of SMED30025195 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
tmem237b	1.031e-7	25.19	transmembrane protein 237B [Source:NCBI gene;Acc:1...	[more]

BLAST of SMED30025195 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30025195 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30025195 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30025195 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2


Match Name	E-value	Identity	Description
tmem237b	1.471e-7	24.11	transmembrane protein 237B [Source:NCBI gene;Acc:1...	[more]
tmem237b	1.755e-7	24.11	transmembrane protein 237B [Source:NCBI gene;Acc:1...	[more]

BLAST of SMED30025195 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1


Match Name	E-value	Identity	Description
SMESG000001693.1	3.885e-45	100.00	SMESG000001693.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30025195 ID=SMED30025195|Name=SMED30025195|organism=Schmidtea mediterranea sexual|type=transcript|length=3067bp
TGTTTAACTAAATCAATTATAAATAATTATGTTATGTGTTATTCAAACAC
CTTAGTTACGTAAATTTATTTTATTGAAAATGTCTGAAAATGTACATGAT
AATCCAGTATATGCTGATGAAAATGCTAATAATGGTGTGAGAAAAAAAAG
ACGCAGAAGACCAAATCCTAATGAAACTGAAACCACCAATACTGTACCAG
AGCCGAACAATGATAATTCCAACGATGTTAAACGTGAACCACGTCGAAGA
CGTCGTAAAGAACGCCGTCAATCGGAAGATGTGAATAACAACGGTCCACT
CGGAGTTGGAGCTTACTTAGAGGATCTACAAGATGATATCGAAATGAAAG
TCAATTCTGATGATTTAAACAATGAAATTTTGACGATATCCAGAGGAAAA
CCTTCTGTATCCCAAGGACCGGGAGATAAAGTCTACATAGAAGGAGTAAG
AAATTTCCGAGTGGAAAATCATGATAAATATTTGAAAAGTCAACAAAAAC
TATCAGAAAAACAAGCCGAAAAATTGAGAATAAATGAAAATGATTACGAT
AACGATTTAATGCAATGTTCAATCGGTGTTACTAATCGATTCAGAATATT
TGCCACATTTCTCCATGGAATACTGGCTGGAATGACACTGATGCACCTGG
TTTTCTGTCTAGTAGTCGGTGGTACTACCCAATCCTCCCAGCAATCCTAT
TTGATATTGTACACCACATTACAGCAGCCGTTCTCAATATTCAATTATGT
CGCCTTCGTTATCATCCTCATATCGCTATGGGATCGTGTCGATGTTGGCC
AGCCGTCACCGAATTGTTCCCAGCCTCAGATTGGAGTTTTGGTGGCATTA
ATTCTGACATTTATTGCTTTTATCATTAACAATAGTCTTTATCTTTACAA
TCAAGACTTCATAAACTTCAACACAAATAGTACCAGTGCGTATTTATCTA
CATTAGTGACTTCATTCGGAACTTGGAAGATTTTGGATGCTGTTAAAAGT
ATCCTTCTGGTTATCAGTTGGTTCATTGTGGCAATGGTTCCAATTTTTGA
TAGGTTTATTGAACAATTAAATGAAAGCATTAATCAAGAGCTAAATGAAA
ATTATTAATTCCTTCTCCTTTCTGGAAAATTATTTTATATTTTCAATTTA
TTGTAAATATTTTGATTATTGCCTTTATAACAAAATTGCAAATTTATTCA
GCATTATAACAAATTTATTTTAGTATTGGATTAAATTTTTCTTGAAAAAA
TAATTCTAATTTATAGAATATTTTGAATTTCTATTCTTTTTTCATTTCAG
ATTTTAGATCGATATTTTGTTTTTATGTATGTTTTTTGTTGAAACTATTG
ATTTTAGTATAATGAAGAAGTGCAAGTAATAGATCTGCTTGCATTTGGGT
GTTGTCGTGATGGATTTTAATATTTCTATGGAATGTTGATGTCCAAATGT
CAACCATAACGGTTGTCATTTCACCATTATGCAATTCGATGCGAGTTGAT
CGATGCACACAGTTGGTACTATATAATGTGCACTAAAGCACGTATGGAGG
TGATGGCATATAGCTGATACTCCACAATTTACTCCATCTTTCTAACTATC
TGTCTATAGATGGATTTTCTCTTTCGGGTCCGGTGTTTATAACCATTTTC
TTGAATATAATTGAGAAGTTAACATTTGTATATCGTCATGTACAAAACAC
CACTAATATAATACACTTCCCTAAACTATGAGTGAACAATAAAACGAACT
TAGACCTCATATATAACGCATTTAGTCCAATTCACTACACTATTTCATAG
TAATATAGGTAGAGAGACAGTGAACTATATCTATTAAAATGAATAAATCT
ATTTCGATTACAATGTAAGTTATGAAATAATTATAAACGAAAAAACGAAT
GTGAAAGTTAGCAAAATACACGATGCATATCTTAAATTAGCATTACAGTA
GAATCGCCATATATGGTACACCTTATACAAGGTACGGCACGATATAAGGT
ACAATTTATTAAAATATACTACAATAAGTTTATACTAAGTCAAAATTCTT
TATCTCTTAGTTCATTATATGTTACACTTGTATAAGGTACAGTTTTTTTA
ATATAAAAATGGTGTACTTTATAGAGAGATTCTACTGTACCTACAATGTA
ATAGTTATGTAGGTAATATTTATGTAGGTCTTACATAAATAAGTATTATA
TAATAGCAAAATTAATTTCATAAGTTGTGATATTACTGCAAACCGACACA
GTCAAATATATATTAGTATGTGCGTGTGATTATAAATCAAATAAAAACCT
CGAATGTAATATTTTAATGTCTCAACTTCTATATTGGTATCAGTTGCGAA
CGGTTCTCCTATATAATGACACGAACCAATTCGGTGTTTTGACTCAAAAC
CTTAGACTTGTTTTCGCTATTTTTTTATATAAACGCGATCTTCATCTTTA
AGCTTCTGTAGTCTTTTCTTGAAACGGTTGAACTTACCTCATACCAGTGG
AGTAATGAGATGCTTGGTCCCTGTAAGACTAAGTAAATCAGTATGGATAG
ATTTTGAGTGAAAGCCTTGTTAATATCACATGATAATATAGAATCTAATA
TTATTCTTTTTTTAATTTTGTAAAGAGCCTTCAAACACTGAGTATTTGAA
GTCAATATATATATTATTTCATAGTGTTTTGCATAATTTTAAAAATGAAT
AGATTTAGTTTTTAAATCAATACTAAGATCACTTATTTTAGTTAACTCTA
TATTAACTTCACTTTGTATATTTGATTGAGCCATGCAAATCTTGCAACGA
CAAATTATCCCACTTCCGGTGATCGAATCGAGCTGAAATTTAGCAGATAA
TTCTTTGAGAACTTTGTATTCTTTCAAAATTTCAGACCGATCGATGGTAT
TATAATTAACTTTTCAATTTGGTAACCTATGGAGAAATGTCTTAACTAGG
TTATTTTGCGTAGTATTAAATATTTCATAGCATTTAGCATGCATGCTGAA
ATTTTAATTTTCAGAGAATGTAAAAGAGTGTGATATAGAGAGATTTAAAA
ACTATGTTTTTACGATT

protein sequence of SMED30025195-orf-1

>SMED30025195-orf-1 ID=SMED30025195-orf-1|Name=SMED30025195-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=343bp
MSENVHDNPVYADENANNGVRKKRRRRPNPNETETTNTVPEPNNDNSNDV
KREPRRRRRKERRQSEDVNNNGPLGVGAYLEDLQDDIEMKVNSDDLNNEI
LTISRGKPSVSQGPGDKVYIEGVRNFRVENHDKYLKSQQKLSEKQAEKLR
INENDYDNDLMQCSIGVTNRFRIFATFLHGILAGMTLMHLVFCLVVGGTT
QSSQQSYLILYTTLQQPFSIFNYVAFVIILISLWDRVDVGQPSPNCSQPQ
IGVLVALILTFIAFIINNSLYLYNQDFINFNTNSTSAYLSTLVTSFGTWK
ILDAVKSILLVISWFIVAMVPIFDRFIEQLNESINQELNENY*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000034	epidermis
PLANA:0000099	neuron
PLANA:0000418	head

Vocabulary: **INTERPRO**
Term	Definition
IPR029409	TMEM237

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 335..342
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..77
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 46..67
None	No IPR available	PANTHER	PTHR28388:SF1	TRANSMEMBRANE PROTEIN 237	coord: 16..341
None	No IPR available	TMHMM	TMhelix		coord: 250..272
None	No IPR available	TMHMM	TMhelix		coord: 301..323
None	No IPR available	TMHMM	TMhelix		coord: 175..197
None	No IPR available	TMHMM	TMhelix		coord: 218..235
IPR029409	Transmembrane protein 237	PFAM	PF15383	TMEM237	coord: 104..324 e-value: 8.4E-35 score: 120.1
IPR029409	Transmembrane protein 237	PANTHER	PTHR28388	FAMILY NOT NAMED	coord: 16..341

Main menu

SMED30025195

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 6

Owlery

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