SMED30025142

Overview
NameSMED30025142
Smed IDSMED30025142
Length (bp)2793
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30025142 (SMED30025142) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30025142 (SMED30025142) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30025142 (SMED30025142) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30025142 (SMED30025142) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30025142

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30025142SMESG000061316.1 SmedASXL_001082SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30025142SMESG000061316.1 SmedASXL_006851SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
prepharyngeal regionSMED30025142SMESG000061316.1 SmedASXL_005910SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parenchymaSMED30025142SMESG000061316.1 dd_Smed_v4_385_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30025142SMESG000061316.1 dd_Smed_v4_4135_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30025142SMESG000061316.1 dd_385dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
parapharyngeal regionSMED30025142SMESG000061316.1 dd_Smed_v6_882_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30025142SMESG000061316.1 dd_Smed_v6_385_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30025142SMESG000061316.1 dd_Smed_v6_4135_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30025142SMESG000061316.1 OX_Smed_1.0.00608ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30025142 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.003279:11617..19067 +13928
Homology
[Close]
BLAST of SMED30025142 vs. Planmine SMEST
Match: SMESG000061316.1 (SMESG000061316.1)

HSP 1 Score: 1469.14 bits (3802), Expect = 0.000e+0
Identity = 832/833 (99.88%), Postives = 833/833 (100.00%), Query Frame = 2 
Query:    2 LVLFKWLLVIPIEGCDLPNSNCNCSQIQDSRYRELVRRYDMRFGGKNGILGFEFGLSRFLSEINTEGLVENISNSIKRYVSISVCNEVRILLNKIVSLINRLNRLETNSKKLEKISNVLIKILKKTFEFESIRRKYFKHIYFINDFKKILNLNESKIKRKYSVIFRHGKKRFSHLNFGSGLIIGGENSLIEIEDGLSRYLTKQILKRLSQNVLRSIKNRLPHEIFKSLKAILKTVTDLIEKLRYLERDVVKLEHESELLFKLHEQNMEFERFHAQYFSEIDWTEIREKNLKGDIEKPNGNEIEENKSYNFQFHEKHGFLELGLFKVYTLSSFDRVIENYIKMMKSKVSQSIIIKVQNLFREIEYLFKGLNSREKNKRKLEYIIHLLYKLNDNNIKFERLRKKYFRELNMIDVFQXXXXXXXXXXXXXXLINQLGGNEGFFGLQQELGELIANLKYHQLEGNVERYIKPKVPRNLWYSXXXXXXXXXXXXXXXXRLEKQHRKFTILSKVLFKLKTKIKTYETYKVKHFXXXXXXXXXXXXXXXXXLETIRKKIYINQNQSKYNIDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNEXXXXXXXXXXXKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVXXXXXXXXXXXXXXXXXXXIDTKHRQHSVKISGKVKT 2500
            LVLFKWLLVIPIEGCDLPNSNCNCSQIQDSRYRELVRRYDMRFGGKNGILGFEFGLSRFLSEINTEGLVENISNSIKRYVSISVCNEVRILLNKIVSLINRLNRLETNSKKLEKISNVLIKILKKTFEFESIRRKYFKHIYFINDFKKILNLNESKIKRKYSVIFRHGKKRFSHLNFGSGLIIGGENSLIEIEDGLSRYLTKQILKRLSQNVLRSIKNRLPHEIFKSLKAILKTVTDLIEKLRYLERDVVKLEHESELLFKLHEQNMEFERFHAQYFSEIDWTEIREKNLKGDIEKPNGNEIEENKSYNFQFHEKHGFLELGLFKVYTLSSFDRVIENYIKMMKSKVSQSIIIKVQNLFREIEYLFKGLNSREKNKRKLEYIIHLLYKLNDNNIKFERLRKKYFRELNMIDVFQKNMKKKNKVSKKRKLINQLGGNEGFFGLQQELGELIANLKYHQLEGNVERYIKPKVPRNLWYSLKKLIKENIEILKKLNRLEKQHRKFTILSKVLFKLKTKIKTYETYKVKHFRKIPIRKIIVKKIREKKLETIRKKIYINQNQSKYNIDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKI+GKVKT
Sbjct:   10 LVLFKWLLVIPIEGCDLPNSNCNCSQIQDSRYRELVRRYDMRFGGKNGILGFEFGLSRFLSEINTEGLVENISNSIKRYVSISVCNEVRILLNKIVSLINRLNRLETNSKKLEKISNVLIKILKKTFEFESIRRKYFKHIYFINDFKKILNLNESKIKRKYSVIFRHGKKRFSHLNFGSGLIIGGENSLIEIEDGLSRYLTKQILKRLSQNVLRSIKNRLPHEIFKSLKAILKTVTDLIEKLRYLERDVVKLEHESELLFKLHEQNMEFERFHAQYFSEIDWTEIREKNLKGDIEKPNGNEIEENKSYNFQFHEKHGFLELGLFKVYTLSSFDRVIENYIKMMKSKVSQSIIIKVQNLFREIEYLFKGLNSREKNKRKLEYIIHLLYKLNDNNIKFERLRKKYFRELNMIDVFQKNMKKKNKVSKKRKLINQLGGNEGFFGLQQELGELIANLKYHQLEGNVERYIKPKVPRNLWYSLKKLIKENIEILKKLNRLEKQHRKFTILSKVLFKLKTKIKTYETYKVKHFRKIPIRKIIVKKIREKKLETIRKKIYINQNQSKYNIDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKITGKVKT 842

HSP 2 Score: 575.474 bits (1482), Expect = 2.593e-179
Identity = 433/877 (49.37%), Postives = 539/877 (61.46%), Query Frame = 2 
Query:  149 LGF-EFGLSRFLSEINTEGLVENISNSIKRYVSISVCNEVRILLNKIVSLINRLNRLETNSKKLEKISNVLIKILKKTFEFESIRRKYFKHIYFINDFKKILNLNESKIKRKYSVIFRHGKKRFSHLNFGSGLIIGGENSLIEIEDGLSRYLTKQILKRLSQNVLRSIKNRLPHEIFKSLKAILKTVTDLIEKLRYLERDVVKLEHESELLFKLHEQNMEFERFHAQYFSEIDWTEIREKNLK-------------------------------------GDIEKP--NGNEIEENKSYNFQFHEKH-------GFLELGLFKVYTLSSFDRVIENYIKMMKSKVSQSIIIKVQNLFREIEYLFKGLNSREKNKRKLEYIIHLLYKLNDNNIKFERLRKKYFRELNMIDVFQXXXXXXXXXXX---XXXLINQLGGNEGFFGLQQELGELIANLKYHQLEGNVERYIKPKVPRNLWYSXXXXXXXXXXXXXXXXRLEKQHRKFTILSKVLFKLKTKIKTYETYKV--------------------------------------KHFXXXXXXXXXXXXXXXXXLETIRKKIYINQNQSK-----YNIDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNEXXXXXXXXXXXKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVXXXXXXXXXXXXXXXXXXXIDTKHRQHSVKISGKVKT 2500
            +GF + G+ R       +   ENI  +IK +   +V  E++ L+ ++ +LI  L + ET+S +L  ISN+L ++ +   +FE +R KYF  +     F    N   +KI+ K   I          L+ G+ L  GG+     + + LS  L +  LK +  NV R +K  +  EI++ ++ +      + EKL  +ERD  KLE  S +LFKL+E+N +FER+  +YF+++    + + N+K                                     G I  P  +G  +   +      H  H       GFL+LG+ +++         EN +K +K     ++I ++Q L  E+  L + L   E +  +L  I +LLY+L+ NNIKFE LR KYF  LN+  +F  +   K +V     K  L  QLGG  GFFGL + L  L+  L+   +  NVER++KP V   +W  ++ L  E+++I +KL  +E+   K   +S +LFKL  K   +E Y++                                      KH +        VK   +     I  KI +   +S+     +++DTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKISGKVKT
Sbjct: 1437 VGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFN---AKIRVKLLNI---------KLSLGAQL--GGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKITGKVKTHGKIHHPKISGKIVVRGRKSRNPHHRFHLDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKITGKVKTHGKIHHPKISGKIVVRGRKSRNPHHRFHLDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKISGKVKT 2299

HSP 3 Score: 560.451 bits (1443), Expect = 5.281e-174
Identity = 426/858 (49.65%), Postives = 531/858 (61.89%), Query Frame = 2 
Query:  149 LGF-EFGLSRFLSEINTEGLVENISNSIKRYVSISVCNEVRILLNKIVSLINRLNRLETNSKKLEKISNVLIKILKKTFEFESIRRKYFKHIYFINDFKKILNLNESKIKRKYSVIFRHGKKRFSHLNFGSGLIIGGENSLIEIEDGLSRYLTKQILKRLSQNVLRSIKNRLPHEIFKSLKAILKTVTDLIEKLRYLERDVVKLEHESELLFKLHEQNMEFERFHAQYFSEIDWTEIREKNLKGDIEKPNG--------NEIEENKSYNFQFHEKHGFLELGLFKVYTLSSFDRVIENYIKMMKSKVSQSIIIK-------------------VQNLFREIEYLFKGLNSREKNKRKLEYIIHLLYKLNDNNIKFERLRKKYFRELNMIDVFQXXXXXXXXXXX---XXXLINQLGGNEGFFGLQQELGELIANLKYHQLEGNVERYIKPKVPRNLWYSXXXXXXXXXXXXXXXXRLEKQHRKFTILSKVLFKLKTKIKTYETYKV--------------------------------------KHFXXXXXXXXXXXXXXXXXLETIRKKIYINQNQSK-----YNIDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNEXXXXXXXXXXXKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVXXXXXXXXXXXXXXXXXXXIDTKHRQHSVKISGKVKT 2500
            +GF + G+ R       +   ENI  +IK +   +V  E++ L+ ++ +LI  L + ET+S +L  ISN+L ++ +   +FE +R KYF  +     F    N   +KI+ K   I          L+ G+ L  GG+     + + LS  L +  LK +  NV R +K  +  EI++ ++ +      + EKL  +ERD  KLE  S +LFKL+E+N +FER+  +YF+++    + + N+K  I++            +I+         H +H     G  K +             K+   K+S  I+++                   +Q L  E+  L + L   E +  +L  I +LLY+L+ NNIKFE LR KYF  LN+  +F  +   K +V     K  L  QLGG  GFFGL + L  L+  L+   +  NVER++KP V   +W  ++ L  E+++I +KL  +E+   K   +S +LFKL  K   +E Y++                                      KH +        VK   +     I  KI +   +S+     +++DTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKI+GKVKT
Sbjct:  877 VGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFN---AKIRVKLLNI---------KLSLGAQL--GGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTK-------HRQHSVKITGKVKTHG------------KIHHPKISGKIVVRGRKSRNPHHRFHLDTRKQEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKITGKVKTHGKIHHPKISGKIVVRGRKSRNPHHRFHLDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKITGKVKT 1701

HSP 4 Score: 493.812 bits (1270), Expect = 1.078e-150
Identity = 397/842 (47.15%), Postives = 517/842 (61.40%), Query Frame = 2 
Query:  149 LGF-EFGLSRFLSEINTEGLVENISNSIKRYVSISVCNEVRILLNKIVSLINRLNRLETNSKKLEKISNVLIKILKKTFEFESIRRKYFKHIYFINDFKKILNLNESKIKRKYSVIFRHGKKRFSHLNFGSGLIIGGENSLIEIEDGLSRYLTKQILKRLSQNVLRSIKNRLPHEIFKSLKAILKTVTDLIEKLRYLERDVVKLEHESELLFKLHEQNMEFERFHAQYFSEIDWTEIREKNLK-------------------------------------GDIEKP--NGNEIEENKSYNFQFHEKH-------GFLELGLFKVYTLSSFDRVIENYIKMMKSKVSQSIIIKVQNLFREIEYLFKGLNSREKNKRKLEYIIHLLYKLNDNNIKFERLRKKYFRELNMIDVFQXXXXXXXXXXX---XXXLINQLGGNEGFFGLQQELGELIANLKYHQLEGNVERYIKPKVPRNLWYSXXXXXXXXXXXXXXXXRLEKQHRKFTILSKVLFKLKTKIKTYETYKVKHFXXXXXXXXXXXXXXXXXLETIRKKIYINQNQSKYNIDTRKQVGFLKLGVERLFLG--FRDKDDTENILKTIKIHAPHNVI------KEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNEXXXXXXXXXXXKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVXXXXXXXXXXXXXXXXXXXIDTKHRQHSVKISGKVKT 2500
            +GF + G+ R       +   ENI  +IK +   +V  E++ L+ ++ +LI  L + ET+S +L  ISN+L ++ +   +FE +R KYF  +     F    N   +KI+ K   I          L+ G+ L  GG+     + + LS  L +  LK +  NV R +K  +  EI++ ++ +      + EKL  +ERD  KLE  S +LFKL+E+N +FER+  +YF+++    + + N+K                                     G I  P  +G  +   +      H  H       GFL+LG+ +++         EN +K +K     ++I ++Q L  E+  L + L   E +  +L  I +LLY+L+ NNIKFE LR KYF  LN+  +F  +   K +V     K  L  QLGG  GFFGL + L  L+  L+   +  NVER++KP V   +W  ++ L  E+++I +KL  +E+   K   +S +LFKL  K   +E Y++K+F K+ ++ +I   ++ K  +    K Y+   +    IDT+ +   +K+  +    G     K   + +++  K   PH+        +EIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKI+GKVKT
Sbjct:  578 VGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFN---AKIRVKLLNI---------KLSLGAQL--GGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKITGKVKTHGKIHHPKISGKIVVRGRKSRNPHHRFHLDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYL---KIVGKIDTKHRQHSVKITGKVKTHGKIHHPKISGKIVVRGRKSRNPHHRFHLDTRKQEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKHRQHSVKITGKVKT 1402

HSP 5 Score: 107.457 bits (267), Expect = 2.902e-23
Identity = 114/410 (27.80%), Postives = 193/410 (47.07%), Query Frame = 2 
Query:  122 MRFGGKNGILGFEFGLSRFLSEINTEGLVENISNSIKRYVSISVCNEVRILLNKIVSLINRLNRLETNSKKLEKISNVLIKILKKTFEFESIRRKYFKHIYFIN-----------------DFKKILNLNESKIKRKYSV-----IFRHGKKRFSHLNFGSGLIIGGENS--------------LIEIEDGLSRYLTKQILKRLSQNVLRSIKNRLPHEIFKSLKAILKTVTDLIEKLRYLERDVVKLEHESELLFKLHEQNMEFERFHAQYFSEIDWTEIREKNLKGDIEKPNGNEIEENKSYNFQFHEKHGFLELG--LFKVYTLSSFDRVIENYIKMMKSKVSQSIIIKVQNLFREIEYLFKGLNSREKNKRKLEYIIHLLYKLNDNNIKFERLRKKYFRELNMIDV 1237
             + GGK G  G    LS  L  +  + ++ N+   +K  VS+ +  E+RIL  + V +  +L  +E +S KLEKISN+L K+ +K  +FE  R KYF  +   N                  + KI+   ++K  R++SV     +  HGK    H      +++ G  S              +  ++ G+ R       K  ++N+L++IK   PH + K ++ ++  +  LI+ L   E D  +L   S LL++LH+ N++FE   A+YFS ++   +   +    I     N      S   Q   K GF  L   L  +        ++ N  + +K  VS  I  +++ LF E   +F+ L + E++  KLE I ++L+KL + N KFER R KYF ++++ +V
Sbjct: 1555 AQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTK-HRQHSVKITGKVKTHGK--IHHPKISGKIVVRGRKSRNPHHRFHLDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLN---IKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNV 1958

HSP 6 Score: 107.457 bits (267), Expect = 2.902e-23
Identity = 114/410 (27.80%), Postives = 193/410 (47.07%), Query Frame = 2 
Query:  122 MRFGGKNGILGFEFGLSRFLSEINTEGLVENISNSIKRYVSISVCNEVRILLNKIVSLINRLNRLETNSKKLEKISNVLIKILKKTFEFESIRRKYFKHIYFIN-----------------DFKKILNLNESKIKRKYSV-----IFRHGKKRFSHLNFGSGLIIGGENS--------------LIEIEDGLSRYLTKQILKRLSQNVLRSIKNRLPHEIFKSLKAILKTVTDLIEKLRYLERDVVKLEHESELLFKLHEQNMEFERFHAQYFSEIDWTEIREKNLKGDIEKPNGNEIEENKSYNFQFHEKHGFLELG--LFKVYTLSSFDRVIENYIKMMKSKVSQSIIIKVQNLFREIEYLFKGLNSREKNKRKLEYIIHLLYKLNDNNIKFERLRKKYFRELNMIDV 1237
             + GGK G  G    LS  L  +  + ++ N+   +K  VS+ +  E+RIL  + V +  +L  +E +S KLEKISN+L K+ +K  +FE  R KYF  +   N                  + KI+   ++K  R++SV     +  HGK    H      +++ G  S              +  ++ G+ R       K  ++N+L++IK   PH + K ++ ++  +  LI+ L   E D  +L   S LL++LH+ N++FE   A+YFS ++   +   +    I     N      S   Q   K GF  L   L  +        ++ N  + +K  VS  I  +++ LF E   +F+ L + E++  KLE I ++L+KL + N KFER R KYF ++++ +V
Sbjct: 1854 AQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTK-HRQHSVKITGKVKTHGK--IHHPKISGKIVVRGRKSRNPHHRFHLDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHAPHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLNI---KLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNV 2257

HSP 7 Score: 92.8189 bits (229), Expect = 9.554e-19
Identity = 105/380 (27.63%), Postives = 176/380 (46.32%), Query Frame = 2 
Query:  122 MRFGGKNGILGFEFGLSRFLSEINTEGLVENISNSIKRYVSISVCNEVRILLNKIVSLINRLNRLETNSKKLEKISNVLIKILKKTFEFESIRRKYFKHIYFINDFKKILNLNESKIKRKYSVIFRHGKKRFSHLNFGSGLIIGGENSLIEIEDGLSRYLTKQILKRLSQNVLRSIKNRLPHEIF------KSLKAILKTVTDLIEKLRYLERDVVKLEHESELLFKLHEQNMEFERFHAQYFSEIDWTEIREKNLKGDIEKPNGNEIEENKSYNFQFHEKHGFLELG--LFKVYTLSSFDRVIENYIKMMKSKVSQSIIIKVQNLFREIEYLFKGLNSREKNKRKLEYIIHLLYKLNDNNIKFERLRKKYFRELNMIDV 1237
             + GGK G  G    LS  L  +  + ++ N+   +K  VS+ +  E+RIL  + V +  +L  +E +S KLEKISN+L K+ +K  +FE  R KYF  +   N     + +   + K     +   GK    H               ++I   +  +      K   + V+R  K+R PH  F      + ++ ++  +  LI+ L   E D  +L   S LL++LH+ N++FE   A+YFS ++   +   +    I     N      S   Q   K GF  L   L  +        ++ N  + +K  VS  I  +++ LF E   +F+ L + E++  KLE I ++L+KL + N KFER R KYF ++++ +V
Sbjct:  995 AQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKIKQKKINKKYLKIVGKIDTKH-----------RQHSVKITGKVKTHGKIHHPKISGKIVVRGRKSRNPHHRFHLDTRKQEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFEMLRAKYFSRLNVKTLFGSSFNAKIRVKLLN---IKLSLGAQLGGKRGFFGLNERLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTTIERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNV 1360
The following BLAST results are available for this feature:
BLAST of SMED30025142 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30025142 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30025142 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30025142 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30025142 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30025142 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30025142 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30025142 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30025142 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30025142 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30025142 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30025142 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30025142 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30025142 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000061316.12.593e-17999.88SMESG000061316.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30025142 ID=SMED30025142|Name=SMED30025142|organism=Schmidtea mediterranea sexual|type=transcript|length=2793bp
ATTAGTTCTTTTCAAGTGGCTGTTGGTCATACCAATTGAGGGATGTGATC
TTCCAAATTCAAATTGTAACTGTTCACAAATTCAAGATTCGAGATATCGC
GAGTTAGTGCGAAGATATGATATGCGATTTGGAGGGAAAAATGGGATACT
GGGATTTGAATTTGGTTTAAGCAGATTTCTTTCCGAAATTAATACTGAAG
GATTAGTAGAAAATATTTCAAATTCAATAAAACGCTATGTATCCATCAGT
GTTTGCAATGAAGTCAGAATACTATTAAATAAAATTGTCAGCCTAATCAA
CCGGCTCAATAGATTGGAAACAAACAGTAAAAAATTAGAAAAAATCAGCA
ATGTTTTGATAAAAATATTGAAGAAAACTTTCGAATTTGAAAGCATTCGA
CGAAAATACTTCAAACATATTTATTTCATAAATGATTTCAAAAAGATTCT
GAATTTAAACGAAAGTAAAATAAAAAGAAAATATTCAGTTATATTCAGAC
ATGGAAAGAAAAGATTTTCTCATTTAAATTTTGGAAGTGGATTGATCATT
GGAGGAGAAAATAGTTTGATTGAAATTGAAGACGGGTTAAGTCGTTATCT
GACTAAACAAATATTAAAGAGATTATCACAAAACGTTTTACGTTCTATCA
AAAATAGATTGCCACACGAAATATTCAAGTCTTTAAAAGCAATCCTTAAA
ACAGTTACAGATTTAATAGAAAAATTACGATATTTAGAAAGAGACGTCGT
AAAACTTGAACATGAGAGTGAATTACTATTCAAGCTTCATGAACAAAACA
TGGAATTCGAAAGATTTCATGCGCAATATTTTTCAGAAATAGATTGGACA
GAAATAAGAGAAAAAAATTTGAAAGGGGATATTGAAAAACCAAACGGAAA
TGAAATTGAAGAAAATAAAAGTTATAACTTCCAATTTCACGAAAAACATG
GGTTTTTGGAACTAGGTTTGTTTAAAGTTTACACACTCTCCAGTTTTGAC
CGAGTGATAGAAAACTACATCAAAATGATGAAATCCAAAGTTTCACAAAG
TATAATAATTAAAGTCCAAAATTTGTTTAGAGAAATAGAATATTTATTCA
AAGGTCTGAATTCAAGAGAAAAAAACAAAAGAAAACTAGAATATATAATT
CACTTGTTATATAAACTGAACGATAATAATATTAAATTCGAAAGATTGCG
AAAGAAATACTTTCGAGAATTAAATATGATTGACGTATTTCAAAAGAATA
TGAAAAAGAAAAATAAAGTATCTAAGAAACGAAAGCTAATTAACCAATTG
GGAGGAAACGAAGGATTTTTTGGGTTGCAACAAGAACTAGGTGAATTAAT
AGCAAATTTAAAATATCATCAACTAGAAGGAAACGTAGAAAGATATATTA
AACCAAAGGTTCCGAGAAACTTGTGGTATTCTTTGAAAAAACTAATAAAA
GAAAATATCGAAATTTTAAAAAAATTAAATAGATTAGAAAAACAACACAG
AAAATTCACGATTTTAAGCAAAGTTCTATTCAAATTAAAAACCAAAATAA
AAACTTATGAAACATACAAAGTAAAGCACTTCAGAAAAATTCCAATAAGA
AAAATAATTGTTAAGAAAATTAGAGAAAAAAAATTAGAAACAATAAGAAA
AAAAATATATATAAATCAAAACCAAAGTAAATATAATATAGACACTCGAA
AACAAGTAGGTTTCTTAAAACTGGGAGTAGAACGATTATTCCTTGGATTC
AGAGACAAAGATGATACTGAAAATATTCTAAAAACAATAAAAATACACGC
CCCACATAACGTCATAAAGGAAATTCAACAACTGGTATGGGAAATGAGAA
CATTGATTCAAACCTTATACAAATATGAGACAGATTCAGATAGATTAATA
ACCATCAGCAATTTGTTATATCGTCTACATCAAAATAATATCAAATTCGA
AATGTTACGGGCAAAATATTTCTCAAGACTCAACGTGAAAACATTGTTCG
GCAGTAGTTTCAATGCAAAGATCCGAGTTAAACTACTCAATATTAAATTA
TCTCTCGGAGCACAACTTGGAGGTAAACGTGGATTCTTTGGATTAAACGA
ACGTCTAAGTAGTTTGCTACCAAGATTAAGATTGAAAAATATCCTTTCGA
ATGTAGAAAGACATTTAAAACCAATTGTTTCCGTCGAAATATGGCAGGAA
ATAAGAATATTATTTATAGAAAGTGTAAAAATATTCGAAAAACTGACAAC
TATTGAAAGAGACTCCGGAAAATTAGAAAAGATCAGCAATATATTATTCA
AACTGTATGAGAAAAATGATAAATTTGAAAGATATCGAATCAAATATTTC
ACAAAAGTCTCACTTCAAAATGTCATTGATACCAATGTTAAAATTAAAAT
TAAACAGAAAAAGATTAATAAAAAATACCTAAAAATTGTTGGTAAAATTG
ATACAAAACATAGACAACATTCCGTTAAAATCTCTGGAAAAGTCAAAACT
CCAGTTAATATTCAAAAACCGAAAGTTGGCGGAAAAGTTGTTATTAAGAG
TGGGAAAATGGAAACAAACCGTAAATATGTTAAAATTAAAACAGTAAAAG
TGAAAACCAATGTCAAAGTTCACAAGCCTCAAAGTATCAGCAAAGGTTCT
GGAAAGGTCTCTGTTAGAAGTGGCAATACTAAGATTGTTGTTAAAGGTCC
AAAGAAAATTTAATAATAAACAAATAAAAGATATAATTGATAAAGTGTTT
ACTGAATAATATTAAGTTTTGAATATTACTATAACTTGTTAGA
back to top

protein sequence of SMED30025142-orf-1

>SMED30025142-orf-1 ID=SMED30025142-orf-1|Name=SMED30025142-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=904bp
LVLFKWLLVIPIEGCDLPNSNCNCSQIQDSRYRELVRRYDMRFGGKNGIL
GFEFGLSRFLSEINTEGLVENISNSIKRYVSISVCNEVRILLNKIVSLIN
RLNRLETNSKKLEKISNVLIKILKKTFEFESIRRKYFKHIYFINDFKKIL
NLNESKIKRKYSVIFRHGKKRFSHLNFGSGLIIGGENSLIEIEDGLSRYL
TKQILKRLSQNVLRSIKNRLPHEIFKSLKAILKTVTDLIEKLRYLERDVV
KLEHESELLFKLHEQNMEFERFHAQYFSEIDWTEIREKNLKGDIEKPNGN
EIEENKSYNFQFHEKHGFLELGLFKVYTLSSFDRVIENYIKMMKSKVSQS
IIIKVQNLFREIEYLFKGLNSREKNKRKLEYIIHLLYKLNDNNIKFERLR
KKYFRELNMIDVFQKNMKKKNKVSKKRKLINQLGGNEGFFGLQQELGELI
ANLKYHQLEGNVERYIKPKVPRNLWYSLKKLIKENIEILKKLNRLEKQHR
KFTILSKVLFKLKTKIKTYETYKVKHFRKIPIRKIIVKKIREKKLETIRK
KIYINQNQSKYNIDTRKQVGFLKLGVERLFLGFRDKDDTENILKTIKIHA
PHNVIKEIQQLVWEMRTLIQTLYKYETDSDRLITISNLLYRLHQNNIKFE
MLRAKYFSRLNVKTLFGSSFNAKIRVKLLNIKLSLGAQLGGKRGFFGLNE
RLSSLLPRLRLKNILSNVERHLKPIVSVEIWQEIRILFIESVKIFEKLTT
IERDSGKLEKISNILFKLYEKNDKFERYRIKYFTKVSLQNVIDTNVKIKI
KQKKINKKYLKIVGKIDTKHRQHSVKISGKVKTPVNIQKPKVGGKVVIKS
GKMETNRKYVKIKTVKVKTNVKVHKPQSISKGSGKVSVRSGNTKIVVKGP
KKI*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000419prepharyngeal region
PLANA:0000420parapharyngeal region
PLANA:0002142parenchyma
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 7..29