SMED30025135

Overview


Name	SMED30025135
Smed ID	SMED30025135
Length (bp)	585

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30025135 (SMED30025135) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30025135 (SMED30025135) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30025135 (SMED30025135) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30025135 (SMED30025135) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30025135

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 13

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
head region	SMED30025135	h1SMcG0015663	SmedASXL_009089	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
head region	SMED30025135	h1SMcG0015663	SmedASXL_017529	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
head region	SMED30025135	h1SMcG0015662	SmedASXL_009089	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
Smed sexual biotype	SMED30025135	h1SMcG0015663	Contig38465	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30025135	h1SMcG0015663	Contig38465	newmark_ests	PMID:29674431 Fincher et al., 2018
nervous system	SMED30025135	h1SMcG0015663	dd_Smed_v4_11981_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30025135	h1SMcG0015663	dd_Smed_v4_11981_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neuron	SMED30025135	h1SMcG0015663	dd_Smed_v4_11981_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30025135	h1SMcG0015663	dd_Smed_v4_11981_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchyma	SMED30025135	h1SMcG0015663	dd_Smed_v4_11981_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30025135	h1SMcG0015663	dd_Smed_v4_11981_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cilated neuron	SMED30025135	h1SMcG0015663	dd_Smed_v4_11981_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
head region	SMED30025135	h1SMcG0015663	dd_Smed_v6_11981_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017

Note: Hover over icons to view figure legend

Alignments

SMED30025135 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.000347:171516..175064 +	2794

Homology

[Close]

BLAST of SMED30025135 vs. Ensembl Human
Match: UNC13B (unc-13 homolog B [Source:HGNC Symbol;Acc:HGNC:12566])

HSP 1 Score: 51.9878 bits (123), Expect = 7.113e-11
Identity = 20/60 (33.33%), Postives = 41/60 (68.33%), Query Frame = -3

Query:  389 ISELAKLLQNLKGSQN---NQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 559
            + ++A +L  ++G+ N   + +A   Q+++ +LRPLM++ D +L+   ++CEKTV KR++
Sbjct: 1614 VRQMADILGQVRGTGNASPDARASAAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVL 1673

HSP 2 Score: 33.113 bits (74), Expect = 7.113e-11
Identity = 12/27 (44.44%), Postives = 20/27 (74.07%), Query Frame = -2

Query:  291 ELWKITMHNLEKQVVLPPLSDPRQVRL 371
            ELW++ M+ +E+ +VLPPL+D    +L
Sbjct: 1675 ELWRVVMNTMERMIVLPPLTDQTGTQL 1701

[Close]

BLAST of SMED30025135 vs. Ensembl Human
Match: UNC13B (unc-13 homolog B [Source:HGNC Symbol;Acc:HGNC:12566])

HSP 1 Score: 51.6026 bits (122), Expect = 1.054e-10
Identity = 20/60 (33.33%), Postives = 41/60 (68.33%), Query Frame = -3

Query:  389 ISELAKLLQNLKGSQN---NQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 559
            + ++A +L  ++G+ N   + +A   Q+++ +LRPLM++ D +L+   ++CEKTV KR++
Sbjct: 3983 VRQMADILGQVRGTGNASPDARASAAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVL 4042

HSP 2 Score: 33.113 bits (74), Expect = 1.054e-10
Identity = 12/27 (44.44%), Postives = 20/27 (74.07%), Query Frame = -2

Query:  291 ELWKITMHNLEKQVVLPPLSDPRQVRL 371
            ELW++ M+ +E+ +VLPPL+D    +L
Sbjct: 4044 ELWRVVMNTMERMIVLPPLTDQTGTQL 4070

[Close]

BLAST of SMED30025135 vs. Ensembl Human
Match: UNC13B (unc-13 homolog B [Source:HGNC Symbol;Acc:HGNC:12566])

HSP 1 Score: 49.2914 bits (116), Expect = 5.024e-10
Identity = 20/60 (33.33%), Postives = 41/60 (68.33%), Query Frame = -3

Query:  389 ISELAKLLQNLKGSQN---NQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 559
            + ++A +L  ++G+ N   + +A   Q+++ +LRPLM++ D +L+   ++CEKTV KR++
Sbjct: 1246 VRQMADILGQVRGTGNASPDARASAAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVL 1305

HSP 2 Score: 33.113 bits (74), Expect = 5.024e-10
Identity = 12/27 (44.44%), Postives = 20/27 (74.07%), Query Frame = -2

Query:  291 ELWKITMHNLEKQVVLPPLSDPRQVRL 371
            ELW++ M+ +E+ +VLPPL+D    +L
Sbjct: 1307 ELWRVVMNTMERMIVLPPLTDQTGTQL 1333

[Close]

BLAST of SMED30025135 vs. Ensembl Human
Match: UNC13B (unc-13 homolog B [Source:HGNC Symbol;Acc:HGNC:12566])

HSP 1 Score: 48.9062 bits (115), Expect = 5.322e-10
Identity = 20/60 (33.33%), Postives = 41/60 (68.33%), Query Frame = -3

Query:  389 ISELAKLLQNLKGSQN---NQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 559
            + ++A +L  ++G+ N   + +A   Q+++ +LRPLM++ D +L+   ++CEKTV KR++
Sbjct:  821 VRQMADILGQVRGTGNASPDARASAAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVL 880

HSP 2 Score: 33.4982 bits (75), Expect = 5.322e-10
Identity = 12/27 (44.44%), Postives = 20/27 (74.07%), Query Frame = -2

Query:  291 ELWKITMHNLEKQVVLPPLSDPRQVRL 371
            ELW++ M+ +E+ +VLPPL+D    +L
Sbjct:  882 ELWRVVMNTMERMIVLPPLTDQTGTQL 908

[Close]

BLAST of SMED30025135 vs. Ensembl Human
Match: UNC13B (unc-13 homolog B [Source:HGNC Symbol;Acc:HGNC:12566])

HSP 1 Score: 48.9062 bits (115), Expect = 5.410e-10
Identity = 20/60 (33.33%), Postives = 41/60 (68.33%), Query Frame = -3

Query:  389 ISELAKLLQNLKGSQN---NQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 559
            + ++A +L  ++G+ N   + +A   Q+++ +LRPLM++ D +L+   ++CEKTV KR++
Sbjct:  821 VRQMADILGQVRGTGNASPDARASAAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVL 880

HSP 2 Score: 33.113 bits (74), Expect = 5.410e-10
Identity = 12/27 (44.44%), Postives = 20/27 (74.07%), Query Frame = -2

Query:  291 ELWKITMHNLEKQVVLPPLSDPRQVRL 371
            ELW++ M+ +E+ +VLPPL+D    +L
Sbjct:  882 ELWRVVMNTMERMIVLPPLTDQTGTQL 908

[Close]

BLAST of SMED30025135 vs. Ensembl Celegans
Match: unc-13 (Phorbol ester/diacylglycerol-binding protein unc-13 [Source:UniProtKB/Swiss-Prot;Acc:P27715])

HSP 1 Score: 40.4318 bits (93), Expect = 1.622e-9
Identity = 21/55 (38.18%), Postives = 32/55 (58.18%), Query Frame = -3

Query:  389 ELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 553
            +L  LL  +KG Q  QK  ++ E++ +L PLM+  + SL      CEKTV K ++
Sbjct: 1088 KLGMLLVKIKGPQL-QKTQVQPEADAVLEPLMDLLEGSLRRYADQCEKTVLKYIL 1141

HSP 2 Score: 38.891 bits (89), Expect = 1.622e-9
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELWKIT+ N+EK+VVLPPLSD
Sbjct: 1143 ELWKITIVNMEKRVVLPPLSD 1163

[Close]

Query:  389 ELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 553
            +L  LL  +KG Q  QK  ++ E++ +L PLM+  + SL      CEKTV K ++
Sbjct: 1085 KLGMLLVKIKGPQL-QKTQVQPEADAVLEPLMDLLEGSLRRYADQCEKTVLKYIL 1138

HSP 2 Score: 38.891 bits (89), Expect = 1.622e-9
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELWKIT+ N+EK+VVLPPLSD
Sbjct: 1140 ELWKITIVNMEKRVVLPPLSD 1160

[Close]

BLAST of SMED30025135 vs. Ensembl Celegans
Match: unc-13 (Phorbol ester/diacylglycerol-binding protein unc-13 [Source:UniProtKB/Swiss-Prot;Acc:P27715])

HSP 1 Score: 40.4318 bits (93), Expect = 1.834e-9
Identity = 21/55 (38.18%), Postives = 32/55 (58.18%), Query Frame = -3

Query:  389 ELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 553
            +L  LL  +KG Q  QK  ++ E++ +L PLM+  + SL      CEKTV K ++
Sbjct: 1436 KLGMLLVKIKGPQL-QKTQVQPEADAVLEPLMDLLEGSLRRYADQCEKTVLKYIL 1489

HSP 2 Score: 38.891 bits (89), Expect = 1.834e-9
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELWKIT+ N+EK+VVLPPLSD
Sbjct: 1491 ELWKITIVNMEKRVVLPPLSD 1511

[Close]

BLAST of SMED30025135 vs. Ensembl Celegans
Match: unc-13 (Phorbol ester/diacylglycerol-binding protein unc-13 [Source:UniProtKB/Swiss-Prot;Acc:P27715])

HSP 1 Score: 40.4318 bits (93), Expect = 1.849e-9
Identity = 21/55 (38.18%), Postives = 32/55 (58.18%), Query Frame = -3

Query:  389 ELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 553
            +L  LL  +KG Q  QK  ++ E++ +L PLM+  + SL      CEKTV K ++
Sbjct: 1433 KLGMLLVKIKGPQL-QKTQVQPEADAVLEPLMDLLEGSLRRYADQCEKTVLKYIL 1486

HSP 2 Score: 38.891 bits (89), Expect = 1.849e-9
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELWKIT+ N+EK+VVLPPLSD
Sbjct: 1488 ELWKITIVNMEKRVVLPPLSD 1508

[Close]

BLAST of SMED30025135 vs. Ensembl Celegans
Match: unc-13 (Phorbol ester/diacylglycerol-binding protein unc-13 [Source:UniProtKB/Swiss-Prot;Acc:P27715])

HSP 1 Score: 40.4318 bits (93), Expect = 1.880e-9
Identity = 21/55 (38.18%), Postives = 32/55 (58.18%), Query Frame = -3

Query:  389 ELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 553
            +L  LL  +KG Q  QK  ++ E++ +L PLM+  + SL      CEKTV K ++
Sbjct: 1436 KLGMLLVKIKGPQL-QKTQVQPEADAVLEPLMDLLEGSLRRYADQCEKTVLKYIL 1489

HSP 2 Score: 38.891 bits (89), Expect = 1.880e-9
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELWKIT+ N+EK+VVLPPLSD
Sbjct: 1491 ELWKITIVNMEKRVVLPPLSD 1511

[Close]

BLAST of SMED30025135 vs. Ensembl Fly
Match: unc-13 (gene:FBgn0025726 transcript:FBtr0344971)

HSP 1 Score: 46.2098 bits (108), Expect = 1.374e-9
Identity = 24/70 (34.29%), Postives = 38/70 (54.29%), Query Frame = -3

Query:  389 QIVANISELAKLLQNLKGSQNN--------QKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 574
            +I  ++ EL  +L ++KG            Q+  +  E++ +LRPLM+  D SL+     CEKTV KRL+
Sbjct: 2476 RITQSVRELGDMLLSIKGGSGTLAAGNLAAQRNAVAVEADEVLRPLMDLLDGSLTLYAQSCEKTVLKRLL 2545

HSP 2 Score: 33.4982 bits (75), Expect = 1.374e-9
Identity = 14/21 (66.67%), Postives = 17/21 (80.95%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELWKI M  LEK +VLPP++D
Sbjct: 2547 ELWKIVMRILEKTIVLPPMTD 2567

[Close]

BLAST of SMED30025135 vs. Ensembl Fly
Match: unc-13 (gene:FBgn0025726 transcript:FBtr0089246)

HSP 1 Score: 46.2098 bits (108), Expect = 1.466e-9
Identity = 24/70 (34.29%), Postives = 38/70 (54.29%), Query Frame = -3

Query:  389 QIVANISELAKLLQNLKGSQNN--------QKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 574
            +I  ++ EL  +L ++KG            Q+  +  E++ +LRPLM+  D SL+     CEKTV KRL+
Sbjct: 2479 RITQSVRELGDMLLSIKGGSGTLAAGNLAAQRNAVAVEADEVLRPLMDLLDGSLTLYAQSCEKTVLKRLL 2548

HSP 2 Score: 33.4982 bits (75), Expect = 1.466e-9
Identity = 14/21 (66.67%), Postives = 17/21 (80.95%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELWKI M  LEK +VLPP++D
Sbjct: 2550 ELWKIVMRILEKTIVLPPMTD 2570

[Close]

BLAST of SMED30025135 vs. Ensembl Fly
Match: unc-13 (gene:FBgn0025726 transcript:FBtr0308805)

HSP 1 Score: 46.2098 bits (108), Expect = 1.466e-9
Identity = 24/70 (34.29%), Postives = 38/70 (54.29%), Query Frame = -3

Query:  389 QIVANISELAKLLQNLKGSQNN--------QKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 574
            +I  ++ EL  +L ++KG            Q+  +  E++ +LRPLM+  D SL+     CEKTV KRL+
Sbjct: 2476 RITQSVRELGDMLLSIKGGSGTLAAGNLAAQRNAVAVEADEVLRPLMDLLDGSLTLYAQSCEKTVLKRLL 2545

HSP 2 Score: 33.4982 bits (75), Expect = 1.466e-9
Identity = 14/21 (66.67%), Postives = 17/21 (80.95%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELWKI M  LEK +VLPP++D
Sbjct: 2547 ELWKIVMRILEKTIVLPPMTD 2567

[Close]

BLAST of SMED30025135 vs. Ensembl Fly
Match: unc-13 (gene:FBgn0025726 transcript:FBtr0089245)

HSP 1 Score: 46.2098 bits (108), Expect = 1.466e-9
Identity = 24/70 (34.29%), Postives = 38/70 (54.29%), Query Frame = -3

Query:  389 QIVANISELAKLLQNLKGSQNN--------QKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 574
            +I  ++ EL  +L ++KG            Q+  +  E++ +LRPLM+  D SL+     CEKTV KRL+
Sbjct: 2476 RITQSVRELGDMLLSIKGGSGTLAAGNLAAQRNAVAVEADEVLRPLMDLLDGSLTLYAQSCEKTVLKRLL 2545

HSP 2 Score: 33.4982 bits (75), Expect = 1.466e-9
Identity = 14/21 (66.67%), Postives = 17/21 (80.95%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELWKI M  LEK +VLPP++D
Sbjct: 2547 ELWKIVMRILEKTIVLPPMTD 2567

[Close]

BLAST of SMED30025135 vs. Ensembl Fly
Match: unc-13 (gene:FBgn0025726 transcript:FBtr0334487)

HSP 1 Score: 46.2098 bits (108), Expect = 1.618e-9
Identity = 24/70 (34.29%), Postives = 38/70 (54.29%), Query Frame = -3

Query:  389 QIVANISELAKLLQNLKGSQNN--------QKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 574
            +I  ++ EL  +L ++KG            Q+  +  E++ +LRPLM+  D SL+     CEKTV KRL+
Sbjct: 2316 RITQSVRELGDMLLSIKGGSGTLAAGNLAAQRNAVAVEADEVLRPLMDLLDGSLTLYAQSCEKTVLKRLL 2385

HSP 2 Score: 33.4982 bits (75), Expect = 1.618e-9
Identity = 14/21 (66.67%), Postives = 17/21 (80.95%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELWKI M  LEK +VLPP++D
Sbjct: 2387 ELWKIVMRILEKTIVLPPMTD 2407

[Close]

BLAST of SMED30025135 vs. Ensembl Zebrafish
Match: CR769778.1 (protein unc-13 homolog C-like [Source:NCBI gene;Acc:110437876])

HSP 1 Score: 46.9802 bits (110), Expect = 1.216e-11
Identity = 21/57 (36.84%), Postives = 36/57 (63.16%), Query Frame = -3

Query:  389 ISELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 559
            + ++ + L  +KG+  N K++   ++E +LRPLM+  D +L     ICEKTV KR++
Sbjct:  385 VKQMNQELVQMKGNAGN-KSNAAMDAETVLRPLMDLLDKNLMLFAKICEKTVLKRVL 440

HSP 2 Score: 40.4318 bits (93), Expect = 1.216e-11
Identity = 15/24 (62.50%), Postives = 20/24 (83.33%), Query Frame = -2

Query:  300 ELWKITMHNLEKQVVLPPLSDPRQ 371
            ELWKI ++ +E+Q+VLPPLSD  Q
Sbjct:  442 ELWKIVLNTIERQIVLPPLSDQTQ 465

[Close]

Query:  389 ISELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 559
            + ++ + L  +KG+  N K++   ++E +LRPLM+  D +L     ICEKTV KR++
Sbjct:  376 VKQMNQELVQMKGNAGN-KSNAAMDAETVLRPLMDLLDKNLMLFAKICEKTVLKRVL 431

HSP 2 Score: 40.4318 bits (93), Expect = 1.216e-11
Identity = 15/24 (62.50%), Postives = 20/24 (83.33%), Query Frame = -2

Query:  300 ELWKITMHNLEKQVVLPPLSDPRQ 371
            ELWKI ++ +E+Q+VLPPLSD  Q
Sbjct:  433 ELWKIVLNTIERQIVLPPLSDQTQ 456

[Close]

BLAST of SMED30025135 vs. Ensembl Zebrafish
Match: UNC13B (unc-13 homolog Bb (C. elegans) [Source:NCBI gene;Acc:100329499])

HSP 1 Score: 56.225 bits (134), Expect = 4.984e-11
Identity = 28/64 (43.75%), Postives = 43/64 (67.19%), Query Frame = -3

Query:  389 SQIVANISELAKLLQNLKGSQN-NQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 577
            S+I   + ++A LL  +KG  N N K  +E +S+ +LRPLM++ DA+L    S+CEKTV KR++
Sbjct: 1214 SRIDDCMRQMASLLYQIKGPVNTNNKNQVEADSDNMLRPLMDFLDANLMLFASVCEKTVLKRVL 1277

HSP 2 Score: 29.261 bits (64), Expect = 4.984e-11
Identity = 11/21 (52.38%), Postives = 17/21 (80.95%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELW+I M++LEK ++LP  +D
Sbjct: 1279 ELWRIVMNSLEKTIILPQGND 1299

[Close]

BLAST of SMED30025135 vs. Ensembl Zebrafish
Match: unc13a (unc-13 homolog A (C. elegans) [Source:ZFIN;Acc:ZDB-GENE-140722-1])

HSP 1 Score: 48.1358 bits (113), Expect = 9.783e-11
Identity = 20/64 (31.25%), Postives = 42/64 (65.62%), Query Frame = -3

Query:  389 IVANISELAKLLQNLKGSQN---NQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 571
            I  N+ ++  +L  +KG+ N   +  + + Q+++ +L+P+M++ D++L+    ICEKTV KR++
Sbjct:  806 IEENVKQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDFLDSNLTLFAKICEKTVLKRVL 869

HSP 2 Score: 36.1946 bits (82), Expect = 9.783e-11
Identity = 16/30 (53.33%), Postives = 23/30 (76.67%), Query Frame = -2

Query:  285 ELWKITMHNLEKQVVLPPLSD-PRQVRLNC 371
            ELWK+ M+ +EK +VLPPL+D   +V+L C
Sbjct:  871 ELWKLVMNTMEKTIVLPPLTDQTGRVKLAC 900

[Close]

BLAST of SMED30025135 vs. Ensembl Zebrafish
Match: unc13a (unc-13 homolog A (C. elegans) [Source:ZFIN;Acc:ZDB-GENE-140722-1])

HSP 1 Score: 48.1358 bits (113), Expect = 1.176e-10
Identity = 20/64 (31.25%), Postives = 42/64 (65.62%), Query Frame = -3

Query:  389 IVANISELAKLLQNLKGSQN---NQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 571
            I  N+ ++  +L  +KG+ N   +  + + Q+++ +L+P+M++ D++L+    ICEKTV KR++
Sbjct: 1370 IEENVKQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDFLDSNLTLFAKICEKTVLKRVL 1433

HSP 2 Score: 35.8094 bits (81), Expect = 1.176e-10
Identity = 16/30 (53.33%), Postives = 23/30 (76.67%), Query Frame = -2

Query:  285 ELWKITMHNLEKQVVLPPLSD-PRQVRLNC 371
            ELWK+ M+ +EK +VLPPL+D   +V+L C
Sbjct: 1435 ELWKLVMNTMEKTIVLPPLTDQTGRVKLAC 1464

[Close]

BLAST of SMED30025135 vs. Ensembl Xenopus
Match: acaa1 (acetyl-CoA acyltransferase 1 [Source:Xenbase;Acc:XB-GENE-963068])

HSP 1 Score: 48.521 bits (114), Expect = 2.020e-10
Identity = 24/55 (43.64%), Postives = 35/55 (63.64%), Query Frame = -3

Query:  389 ELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 553
            EL ++  N   S N   A ++  +EI+LRPLM++ D +LS    ICEKTV KR++
Sbjct: 1366 ELNQMKGNGNASTNRNNAAMD--AEIVLRPLMDFLDKTLSVSAKICEKTVLKRVL 1418

HSP 2 Score: 35.4242 bits (80), Expect = 2.020e-10
Identity = 13/21 (61.90%), Postives = 19/21 (90.48%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELWK+ ++ +EKQ+VLPPL+D
Sbjct: 1420 ELWKLVLNRIEKQIVLPPLTD 1440

[Close]

BLAST of SMED30025135 vs. Ensembl Xenopus
Match: acaa1 (acetyl-CoA acyltransferase 1 [Source:Xenbase;Acc:XB-GENE-963068])

HSP 1 Score: 48.521 bits (114), Expect = 2.039e-10
Identity = 24/55 (43.64%), Postives = 35/55 (63.64%), Query Frame = -3

Query:  389 ELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 553
            EL ++  N   S N   A ++  +EI+LRPLM++ D +LS    ICEKTV KR++
Sbjct: 1863 ELNQMKGNGNASTNRNNAAMD--AEIVLRPLMDFLDKTLSVSAKICEKTVLKRVL 1915

HSP 2 Score: 35.039 bits (79), Expect = 2.039e-10
Identity = 13/21 (61.90%), Postives = 19/21 (90.48%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELWK+ ++ +EKQ+VLPPL+D
Sbjct: 1917 ELWKLVLNRIEKQIVLPPLTD 1937

[Close]

BLAST of SMED30025135 vs. Ensembl Xenopus
Match: acaa1 (acetyl-CoA acyltransferase 1 [Source:Xenbase;Acc:XB-GENE-963068])

HSP 1 Score: 48.521 bits (114), Expect = 2.332e-10
Identity = 24/55 (43.64%), Postives = 35/55 (63.64%), Query Frame = -3

Query:  389 ELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 553
            EL ++  N   S N   A ++  +EI+LRPLM++ D +LS    ICEKTV KR++
Sbjct: 1510 ELNQMKGNGNASTNRNNAAMD--AEIVLRPLMDFLDKTLSVSAKICEKTVLKRVL 1562

HSP 2 Score: 35.039 bits (79), Expect = 2.332e-10
Identity = 13/21 (61.90%), Postives = 19/21 (90.48%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELWK+ ++ +EKQ+VLPPL+D
Sbjct: 1564 ELWKLVLNRIEKQIVLPPLTD 1584

[Close]

BLAST of SMED30025135 vs. Ensembl Xenopus
Match: acaa1 (acetyl-CoA acyltransferase 1 [Source:Xenbase;Acc:XB-GENE-963068])

HSP 1 Score: 48.521 bits (114), Expect = 2.457e-10
Identity = 24/55 (43.64%), Postives = 35/55 (63.64%), Query Frame = -3

Query:  389 ELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 553
            EL ++  N   S N   A ++  +EI+LRPLM++ D +LS    ICEKTV KR++
Sbjct:  882 ELNQMKGNGNASTNRNNAAMD--AEIVLRPLMDFLDKTLSVSAKICEKTVLKRVL 934

HSP 2 Score: 35.039 bits (79), Expect = 2.457e-10
Identity = 13/21 (61.90%), Postives = 19/21 (90.48%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELWK+ ++ +EKQ+VLPPL+D
Sbjct:  936 ELWKLVLNRIEKQIVLPPLTD 956

[Close]

BLAST of SMED30025135 vs. Ensembl Xenopus
Match: acaa1 (acetyl-CoA acyltransferase 1 [Source:Xenbase;Acc:XB-GENE-963068])

HSP 1 Score: 48.1358 bits (113), Expect = 2.616e-10
Identity = 24/55 (43.64%), Postives = 35/55 (63.64%), Query Frame = -3

Query:  389 ELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 553
            EL ++  N   S N   A ++  +EI+LRPLM++ D +LS    ICEKTV KR++
Sbjct: 1026 ELNQMKGNGNASTNRNNAAMD--AEIVLRPLMDFLDKTLSVSAKICEKTVLKRVL 1078

HSP 2 Score: 35.039 bits (79), Expect = 2.616e-10
Identity = 13/21 (61.90%), Postives = 19/21 (90.48%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELWK+ ++ +EKQ+VLPPL+D
Sbjct: 1080 ELWKLVLNRIEKQIVLPPLTD 1100

[Close]

BLAST of SMED30025135 vs. Ensembl Mouse
Match: Unc13b (unc-13 homolog B [Source:MGI Symbol;Acc:MGI:1342278])

HSP 1 Score: 55.0694 bits (131), Expect = 9.809e-12
Identity = 21/60 (35.00%), Postives = 42/60 (70.00%), Query Frame = -3

Query:  389 ISELAKLLQNLKGSQN---NQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 559
            + ++A +L  ++G+ N   N +A + Q+++ +LRPLM++ D +L+   ++CEKTV KR++
Sbjct: 1606 VRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVL 1665

HSP 2 Score: 32.3426 bits (72), Expect = 9.809e-12
Identity = 12/27 (44.44%), Postives = 20/27 (74.07%), Query Frame = -2

Query:  291 ELWKITMHNLEKQVVLPPLSDPRQVRL 371
            ELW++ M+ +E+ +VLPPL+D    +L
Sbjct: 1667 ELWRVVMNTMERVIVLPPLTDQTGTQL 1693

[Close]

BLAST of SMED30025135 vs. Ensembl Mouse
Match: Unc13b (unc-13 homolog B [Source:MGI Symbol;Acc:MGI:1342278])

HSP 1 Score: 54.6842 bits (130), Expect = 1.565e-11
Identity = 21/60 (35.00%), Postives = 42/60 (70.00%), Query Frame = -3

Query:  389 ISELAKLLQNLKGSQN---NQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 559
            + ++A +L  ++G+ N   N +A + Q+++ +LRPLM++ D +L+   ++CEKTV KR++
Sbjct: 4033 VRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVL 4092

HSP 2 Score: 32.3426 bits (72), Expect = 1.565e-11
Identity = 12/27 (44.44%), Postives = 20/27 (74.07%), Query Frame = -2

Query:  291 ELWKITMHNLEKQVVLPPLSDPRQVRL 371
            ELW++ M+ +E+ +VLPPL+D    +L
Sbjct: 4094 ELWRVVMNTMERVIVLPPLTDQTGTQL 4120

[Close]

BLAST of SMED30025135 vs. Ensembl Mouse
Match: Unc13b (unc-13 homolog B [Source:MGI Symbol;Acc:MGI:1342278])

HSP 1 Score: 52.373 bits (124), Expect = 6.455e-11
Identity = 21/60 (35.00%), Postives = 42/60 (70.00%), Query Frame = -3

Query:  389 ISELAKLLQNLKGSQN---NQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 559
            + ++A +L  ++G+ N   N +A + Q+++ +LRPLM++ D +L+   ++CEKTV KR++
Sbjct: 1244 VRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVL 1303

HSP 2 Score: 32.3426 bits (72), Expect = 6.455e-11
Identity = 12/27 (44.44%), Postives = 20/27 (74.07%), Query Frame = -2

Query:  291 ELWKITMHNLEKQVVLPPLSDPRQVRL 371
            ELW++ M+ +E+ +VLPPL+D    +L
Sbjct: 1305 ELWRVVMNTMERVIVLPPLTDQTGTQL 1331

[Close]

BLAST of SMED30025135 vs. Ensembl Mouse
Match: Unc13b (unc-13 homolog B [Source:MGI Symbol;Acc:MGI:1342278])

HSP 1 Score: 52.373 bits (124), Expect = 6.560e-11
Identity = 21/60 (35.00%), Postives = 42/60 (70.00%), Query Frame = -3

Query:  389 ISELAKLLQNLKGSQN---NQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 559
            + ++A +L  ++G+ N   N +A + Q+++ +LRPLM++ D +L+   ++CEKTV KR++
Sbjct: 1245 VRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVL 1304

HSP 2 Score: 32.3426 bits (72), Expect = 6.560e-11
Identity = 12/27 (44.44%), Postives = 20/27 (74.07%), Query Frame = -2

Query:  291 ELWKITMHNLEKQVVLPPLSDPRQVRL 371
            ELW++ M+ +E+ +VLPPL+D    +L
Sbjct: 1306 ELWRVVMNTMERVIVLPPLTDQTGTQL 1332

[Close]

BLAST of SMED30025135 vs. Ensembl Mouse
Match: Unc13b (unc-13 homolog B [Source:MGI Symbol;Acc:MGI:1342278])

HSP 1 Score: 52.373 bits (124), Expect = 7.057e-11
Identity = 21/60 (35.00%), Postives = 42/60 (70.00%), Query Frame = -3

Query:  389 ISELAKLLQNLKGSQN---NQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 559
            + ++A +L  ++G+ N   N +A + Q+++ +LRPLM++ D +L+   ++CEKTV KR++
Sbjct: 1233 VRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVL 1292

HSP 2 Score: 32.3426 bits (72), Expect = 7.057e-11
Identity = 12/27 (44.44%), Postives = 20/27 (74.07%), Query Frame = -2

Query:  291 ELWKITMHNLEKQVVLPPLSDPRQVRL 371
            ELW++ M+ +E+ +VLPPL+D    +L
Sbjct: 1294 ELWRVVMNTMERVIVLPPLTDQTGTQL 1320

[Close]

BLAST of SMED30025135 vs. UniProt/SwissProt
Match: sp|Q9Z1N9|UN13B_MOUSE (Protein unc-13 homolog B OS=Mus musculus OX=10090 GN=Unc13b PE=1 SV=2)

HSP 1 Score: 52.373 bits (124), Expect = 3.576e-10
Identity = 21/60 (35.00%), Postives = 42/60 (70.00%), Query Frame = -3

Query:  389 ISELAKLLQNLKGSQN---NQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 559
            + ++A +L  ++G+ N   N +A + Q+++ +LRPLM++ D +L+   ++CEKTV KR++
Sbjct: 1245 VRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVL 1304

HSP 2 Score: 32.3426 bits (72), Expect = 3.576e-10
Identity = 12/27 (44.44%), Postives = 20/27 (74.07%), Query Frame = -2

Query:  291 ELWKITMHNLEKQVVLPPLSDPRQVRL 371
            ELW++ M+ +E+ +VLPPL+D    +L
Sbjct: 1306 ELWRVVMNTMERVIVLPPLTDQTGTQL 1332

[Close]

BLAST of SMED30025135 vs. UniProt/SwissProt
Match: sp|Q62769|UN13B_RAT (Protein unc-13 homolog B OS=Rattus norvegicus OX=10116 GN=Unc13b PE=1 SV=2)

HSP 1 Score: 52.7582 bits (125), Expect = 1.011e-9
Identity = 21/60 (35.00%), Postives = 42/60 (70.00%), Query Frame = -3

Query:  389 ISELAKLLQNLKGSQN---NQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 559
            + ++A +L  ++G+ N   N +A + Q+++ +LRPLM++ D +L+   ++CEKTV KR++
Sbjct: 1246 VRQMADILGQVRGTGNASPNARASVAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVL 1305

HSP 2 Score: 30.8018 bits (68), Expect = 1.011e-9
Identity = 12/27 (44.44%), Postives = 19/27 (70.37%), Query Frame = -2

Query:  291 ELWKITMHNLEKQVVLPPLSDPRQVRL 371
            ELW++ M+ +E+ +VLPPL D    +L
Sbjct: 1307 ELWRVVMNTMERVIVLPPLIDQTGTQL 1333

[Close]

BLAST of SMED30025135 vs. UniProt/SwissProt
Match: sp|Q8K0T7|UN13C_MOUSE (Protein unc-13 homolog C OS=Mus musculus OX=10090 GN=Unc13c PE=1 SV=3)

HSP 1 Score: 46.9802 bits (110), Expect = 2.239e-9
Identity = 23/56 (41.07%), Postives = 36/56 (64.29%), Query Frame = -3

Query:  389 SELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 556
            +EL ++  N   + N   A ++  +EI+LRPLM++ D +LS    ICEKTV KR++
Sbjct: 1859 AELNQMRANGNSTANKNSAAMD--AEIVLRPLMDFLDKTLSLSAKICEKTVLKRVL 1912

HSP 2 Score: 35.039 bits (79), Expect = 2.239e-9
Identity = 13/21 (61.90%), Postives = 19/21 (90.48%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELWK+ ++ +EKQ+VLPPL+D
Sbjct: 1914 ELWKLVLNKIEKQIVLPPLTD 1934

[Close]

BLAST of SMED30025135 vs. UniProt/SwissProt
Match: sp|O14795|UN13B_HUMAN (Protein unc-13 homolog B OS=Homo sapiens OX=9606 GN=UNC13B PE=1 SV=2)

HSP 1 Score: 48.9062 bits (115), Expect = 2.242e-9
Identity = 20/60 (33.33%), Postives = 41/60 (68.33%), Query Frame = -3

Query:  389 ISELAKLLQNLKGSQN---NQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 559
            + ++A +L  ++G+ N   + +A   Q+++ +LRPLM++ D +L+   ++CEKTV KR++
Sbjct: 1234 VRQMADILGQVRGTGNASPDARASAAQDADSVLRPLMDFLDGNLTLFATVCEKTVLKRVL 1293

HSP 2 Score: 33.113 bits (74), Expect = 2.242e-9
Identity = 12/27 (44.44%), Postives = 20/27 (74.07%), Query Frame = -2

Query:  291 ELWKITMHNLEKQVVLPPLSDPRQVRL 371
            ELW++ M+ +E+ +VLPPL+D    +L
Sbjct: 1295 ELWRVVMNTMERMIVLPPLTDQTGTQL 1321

[Close]

BLAST of SMED30025135 vs. UniProt/SwissProt
Match: sp|P27715|UNC13_CAEEL (Phorbol ester/diacylglycerol-binding protein unc-13 OS=Caenorhabditis elegans OX=6239 GN=unc-13 PE=1 SV=4)

HSP 1 Score: 40.0466 bits (92), Expect = 1.905e-8
Identity = 21/55 (38.18%), Postives = 32/55 (58.18%), Query Frame = -3

Query:  389 ELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 553
            +L  LL  +KG Q  QK  ++ E++ +L PLM+  + SL      CEKTV K ++
Sbjct: 1775 KLGMLLVKIKGPQL-QKTQVQPEADAVLEPLMDLLEGSLRRYADQCEKTVLKYIL 1828

HSP 2 Score: 38.891 bits (89), Expect = 1.905e-8
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELWKIT+ N+EK+VVLPPLSD
Sbjct: 1830 ELWKITIVNMEKRVVLPPLSD 1850

[Close]

BLAST of SMED30025135 vs. TrEMBL
Match: A0A0L8G286 (Uncharacterized protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22001872mg PE=4 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.399e-16
Identity = 34/68 (50.00%), Postives = 47/68 (69.12%), Query Frame = -3

Query:  389 QIVANISELAKLLQNLKGS------QNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 574
            QI  +I EL KLL N+KG+      Q +Q+++I+ ES++ILRPLM+  D SLS  +  CEKTV KRL+
Sbjct:  223 QIQQSIQELGKLLSNVKGTGQVSLAQPSQRSNIQGESDMILRPLMDLLDGSLSMFSQACEKTVLKRLL 290

HSP 2 Score: 47.3654 bits (111), Expect = 1.399e-16
Identity = 21/27 (77.78%), Postives = 23/27 (85.19%), Query Frame = -2

Query:  291 ELWKITMHNLEKQVVLPPLSDPRQVRL 371
            ELWKI MH +EK VVLPPLSDPRQ+ L
Sbjct:  292 ELWKIVMHTMEKTVVLPPLSDPRQLLL 318

[Close]

BLAST of SMED30025135 vs. TrEMBL
Match: A0A0L8G1T3 (Uncharacterized protein (Fragment) OS=Octopus bimaculoides OX=37653 GN=OCBIM_22001872mg PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.453e-16
Identity = 34/68 (50.00%), Postives = 47/68 (69.12%), Query Frame = -3

Query:  389 QIVANISELAKLLQNLKGS------QNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 574
            QI  +I EL KLL N+KG+      Q +Q+++I+ ES++ILRPLM+  D SLS  +  CEKTV KRL+
Sbjct:  585 QIQQSIQELGKLLSNVKGTGQVSLAQPSQRSNIQGESDMILRPLMDLLDGSLSMFSQACEKTVLKRLL 652

HSP 2 Score: 47.3654 bits (111), Expect = 1.453e-16
Identity = 21/27 (77.78%), Postives = 23/27 (85.19%), Query Frame = -2

Query:  291 ELWKITMHNLEKQVVLPPLSDPRQVRL 371
            ELWKI MH +EK VVLPPLSDPRQ+ L
Sbjct:  654 ELWKIVMHTMEKTVVLPPLSDPRQLLL 680

[Close]

BLAST of SMED30025135 vs. TrEMBL
Match: A0A0L8G1T7 (Uncharacterized protein (Fragment) OS=Octopus bimaculoides OX=37653 GN=OCBIM_22001872mg PE=4 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.503e-16
Identity = 34/68 (50.00%), Postives = 47/68 (69.12%), Query Frame = -3

Query:  389 QIVANISELAKLLQNLKGS------QNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 574
            QI  +I EL KLL N+KG+      Q +Q+++I+ ES++ILRPLM+  D SLS  +  CEKTV KRL+
Sbjct:  585 QIQQSIQELGKLLSNVKGTGQVSLAQPSQRSNIQGESDMILRPLMDLLDGSLSMFSQACEKTVLKRLL 652

HSP 2 Score: 47.3654 bits (111), Expect = 1.503e-16
Identity = 21/27 (77.78%), Postives = 23/27 (85.19%), Query Frame = -2

Query:  291 ELWKITMHNLEKQVVLPPLSDPRQVRL 371
            ELWKI MH +EK VVLPPLSDPRQ+ L
Sbjct:  654 ELWKIVMHTMEKTVVLPPLSDPRQLLL 680

[Close]

BLAST of SMED30025135 vs. TrEMBL
Match: A0A0L8G247 (Uncharacterized protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22001872mg PE=4 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.584e-16
Identity = 34/68 (50.00%), Postives = 47/68 (69.12%), Query Frame = -3

Query:  389 QIVANISELAKLLQNLKGS------QNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 574
            QI  +I EL KLL N+KG+      Q +Q+++I+ ES++ILRPLM+  D SLS  +  CEKTV KRL+
Sbjct:  223 QIQQSIQELGKLLSNVKGTGQVSLAQPSQRSNIQGESDMILRPLMDLLDGSLSMFSQACEKTVLKRLL 290

HSP 2 Score: 47.3654 bits (111), Expect = 1.584e-16
Identity = 21/27 (77.78%), Postives = 23/27 (85.19%), Query Frame = -2

Query:  291 ELWKITMHNLEKQVVLPPLSDPRQVRL 371
            ELWKI MH +EK VVLPPLSDPRQ+ L
Sbjct:  292 ELWKIVMHTMEKTVVLPPLSDPRQLLL 318

[Close]

BLAST of SMED30025135 vs. TrEMBL
Match: A0A5K3F6A9 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 9.421e-16
Identity = 31/62 (50.00%), Postives = 45/62 (72.58%), Query Frame = -3

Query:  389 QIVANISELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 574
            QI+A + E+ KLL  +    +N KA IE E+++ILRPLM++F++SLS    ICEKTV KR++
Sbjct:  543 QILAKVKEVNKLLHQI---SSNSKASIESEADLILRPLMDHFESSLSVYADICEKTVLKRIL 601

HSP 2 Score: 46.9802 bits (110), Expect = 9.421e-16
Identity = 20/28 (71.43%), Postives = 23/28 (82.14%), Query Frame = -2

Query:  288 ELWKITMHNLEKQVVLPPLSDPRQVRLN 371
            ELWKITM+  EKQVVLPP+SDP  + LN
Sbjct:  603 ELWKITMYTFEKQVVLPPVSDPSALFLN 630

[Close]

BLAST of SMED30025135 vs. Ensembl Cavefish
Match: ENSAMXT00000045754.1 (pep primary_assembly:Astyanax_mexicanus-2.0:20:15823441:15932673:1 gene:ENSAMXG00000017447.2 transcript:ENSAMXT00000045754.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 54.6842 bits (130), Expect = 8.623e-11
Identity = 24/64 (37.50%), Postives = 43/64 (67.19%), Query Frame = -3

Query:  389 SQIVANISELAKLLQNLKGSQN-NQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 577
            ++I   + ++A LL  +KG  N N +  +E +++ +LRPLM++ D +L+   S+CEKTV KR++
Sbjct: 1342 TRIDECMRQMASLLYQIKGPVNANSRNQVEGDADNVLRPLMDFLDGNLTLFASVCEKTVLKRVL 1405

HSP 2 Score: 29.6462 bits (65), Expect = 8.623e-11
Identity = 12/21 (57.14%), Postives = 17/21 (80.95%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELW+I M++LEK +VLP  +D
Sbjct: 1407 ELWRIVMNSLEKTIVLPQGND 1427

[Close]

BLAST of SMED30025135 vs. Ensembl Cavefish
Match: ENSAMXT00000036882.1 (pep primary_assembly:Astyanax_mexicanus-2.0:20:15877557:15935246:1 gene:ENSAMXG00000017447.2 transcript:ENSAMXT00000036882.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 52.7582 bits (125), Expect = 1.602e-10
Identity = 23/58 (39.66%), Postives = 40/58 (68.97%), Query Frame = -3

Query:  389 ISELAKLLQNLKGSQN-NQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 559
            + ++A LL  +KG  N N +  +E +++ +LRPLM++ D +L+   S+CEKTV KR++
Sbjct: 1702 MRQMASLLYQIKGPVNANSRNQVEGDADNVLRPLMDFLDGNLTLFASVCEKTVLKRVL 1759

HSP 2 Score: 30.4166 bits (67), Expect = 1.602e-10
Identity = 12/21 (57.14%), Postives = 17/21 (80.95%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELW+I M++LEK +VLP  +D
Sbjct: 1761 ELWRIVMNSLEKTIVLPQGND 1781

[Close]

BLAST of SMED30025135 vs. Ensembl Cavefish
Match: ENSAMXT00000049675.1 (pep primary_assembly:Astyanax_mexicanus-2.0:20:15823549:15937399:1 gene:ENSAMXG00000017447.2 transcript:ENSAMXT00000049675.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.5286 bits (127), Expect = 1.912e-10
Identity = 23/58 (39.66%), Postives = 40/58 (68.97%), Query Frame = -3

Query:  389 ISELAKLLQNLKGSQN-NQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 559
            + ++A LL  +KG  N N +  +E +++ +LRPLM++ D +L+   S+CEKTV KR++
Sbjct: 1218 MRQMASLLYQIKGPVNANSRNQVEGDADNVLRPLMDFLDGNLTLFASVCEKTVLKRVL 1275

HSP 2 Score: 29.6462 bits (65), Expect = 1.912e-10
Identity = 12/21 (57.14%), Postives = 17/21 (80.95%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELW+I M++LEK +VLP  +D
Sbjct: 1277 ELWRIVMNSLEKTIVLPQGND 1297

[Close]

BLAST of SMED30025135 vs. Ensembl Cavefish
Match: ENSAMXT00000017969.2 (pep primary_assembly:Astyanax_mexicanus-2.0:20:15794227:15935246:1 gene:ENSAMXG00000017447.2 transcript:ENSAMXT00000017969.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.1434 bits (126), Expect = 2.525e-10
Identity = 23/58 (39.66%), Postives = 40/58 (68.97%), Query Frame = -3

Query:  389 ISELAKLLQNLKGSQN-NQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 559
            + ++A LL  +KG  N N +  +E +++ +LRPLM++ D +L+   S+CEKTV KR++
Sbjct: 1227 MRQMASLLYQIKGPVNANSRNQVEGDADNVLRPLMDFLDGNLTLFASVCEKTVLKRVL 1284

HSP 2 Score: 29.6462 bits (65), Expect = 2.525e-10
Identity = 12/21 (57.14%), Postives = 17/21 (80.95%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELW+I M++LEK +VLP  +D
Sbjct: 1286 ELWRIVMNSLEKTIVLPQGND 1306

[Close]

BLAST of SMED30025135 vs. Ensembl Cavefish
Match: UNC13C (unc-13 homolog C [Source:HGNC Symbol;Acc:HGNC:23149])

HSP 1 Score: 44.2838 bits (103), Expect = 9.081e-10
Identity = 21/57 (36.84%), Postives = 36/57 (63.16%), Query Frame = -3

Query:  389 ISELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 559
            + ++ + L  +KG+  N K++   ++E +LRPLM+  D +L     ICEKTV KR++
Sbjct: 1794 VKQMNQELVQMKGTAGN-KSNAAMDAETVLRPLMDLLDKNLILFAKICEKTVLKRVL 1849

HSP 2 Score: 36.5798 bits (83), Expect = 9.081e-10
Identity = 14/24 (58.33%), Postives = 20/24 (83.33%), Query Frame = -2

Query:  300 ELWKITMHNLEKQVVLPPLSDPRQ 371
            ELWKI ++ +E+Q+VLPPL+D  Q
Sbjct: 1851 ELWKIVLNTIERQIVLPPLTDQTQ 1874

[Close]

BLAST of SMED30025135 vs. Ensembl Nematostella
Match: EDO30233 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T1D8])

HSP 1 Score: 45.0542 bits (105), Expect = 1.226e-7
Identity = 20/39 (51.28%), Postives = 29/39 (74.36%), Query Frame = -3

Query:  389 KAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 505
            +A +EQES +IL+PLM+  + +LS L   CEK+V KRL+
Sbjct:  162 RAAVEQESAMILQPLMDILEGTLSLLNRACEKSVLKRLL 200

HSP 2 Score: 26.5646 bits (57), Expect = 1.226e-7
Identity = 11/21 (52.38%), Postives = 15/21 (71.43%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            +LWK  + N E+ VVLPP +D
Sbjct:  203 QLWKSLLANFERIVVLPPPND 223

[Close]

BLAST of SMED30025135 vs. Ensembl Medaka
Match: ENSORLT00000005973.2 (unc-13 homolog A [Source:NCBI gene;Acc:101164968])

HSP 1 Score: 50.0618 bits (118), Expect = 4.855e-11
Identity = 21/63 (33.33%), Postives = 40/63 (63.49%), Query Frame = -3

Query:  389 QIVANISELAKLLQNLKGSQN-NQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 574
             I  N+ ++  +L  +KG  N    + + Q+++ +L+P+ME+ D++L+    ICEKTV KR++
Sbjct: 1159 HIEENVKQMGDILAQVKGGSNVGNASSVAQDADNVLQPIMEFLDSNLTLFAKICEKTVLKRVL 1221

HSP 2 Score: 35.039 bits (79), Expect = 4.855e-11
Identity = 14/27 (51.85%), Postives = 20/27 (74.07%), Query Frame = -2

Query:  291 ELWKITMHNLEKQVVLPPLSDPRQVRL 371
            ELWK+ M+ +EK +VLPPL+D    +L
Sbjct: 1223 ELWKLVMNTMEKTIVLPPLTDQTGTQL 1249

[Close]

BLAST of SMED30025135 vs. Ensembl Medaka
Match: UNC13A (protein unc-13 homolog A [Source:NCBI gene;Acc:105356230])

HSP 1 Score: 51.9878 bits (123), Expect = 1.681e-10
Identity = 22/62 (35.48%), Postives = 39/62 (62.90%), Query Frame = -3

Query:  389 QIVANISELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 574
            QI  N+ ++  +L  +KG        + QE++ +L+P+M++ D++LS    ICEKTV KR++
Sbjct: 1174 QIEDNVRQMGDILAQVKGQTVPANGSVVQEADNVLQPIMDFLDSNLSLFAKICEKTVLKRVL 1235

HSP 2 Score: 31.187 bits (69), Expect = 1.681e-10
Identity = 12/21 (57.14%), Postives = 17/21 (80.95%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELWK+ M+ +EK +VLP L+D
Sbjct: 1237 ELWKLVMNTMEKTIVLPTLTD 1257

[Close]

BLAST of SMED30025135 vs. Ensembl Medaka
Match: UNC13A (protein unc-13 homolog A [Source:NCBI gene;Acc:105356230])

HSP 1 Score: 51.6026 bits (122), Expect = 3.072e-10
Identity = 22/62 (35.48%), Postives = 39/62 (62.90%), Query Frame = -3

Query:  389 QIVANISELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 574
            QI  N+ ++  +L  +KG        + QE++ +L+P+M++ D++LS    ICEKTV KR++
Sbjct: 1498 QIEDNVRQMGDILAQVKGQTVPANGSVVQEADNVLQPIMDFLDSNLSLFAKICEKTVLKRVL 1559

HSP 2 Score: 30.8018 bits (68), Expect = 3.072e-10
Identity = 12/21 (57.14%), Postives = 17/21 (80.95%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELWK+ M+ +EK +VLP L+D
Sbjct: 1561 ELWKLVMNTMEKTIVLPTLTD 1581

[Close]

BLAST of SMED30025135 vs. Ensembl Medaka
Match: UNC13B (unc-13 homolog B [Source:NCBI gene;Acc:101167071])

HSP 1 Score: 53.1434 bits (126), Expect = 4.315e-10
Identity = 23/58 (39.66%), Postives = 39/58 (67.24%), Query Frame = -3

Query:  389 ISELAKLLQNLKGSQN-NQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 559
            + ++A LL  +KG  N N K  ++ +S+ +LRPLM++ D  L+   ++CEKTV KR++
Sbjct: 1147 MRQMASLLYQIKGPLNGNTKNQVDADSDNMLRPLMDFLDGKLTLFATVCEKTVLKRVL 1204

HSP 2 Score: 28.4906 bits (62), Expect = 4.315e-10
Identity = 12/21 (57.14%), Postives = 16/21 (76.19%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            ELW+I M +LEK +VLP  +D
Sbjct: 1206 ELWRIVMTSLEKTIVLPQGND 1226

[Close]

BLAST of SMED30025135 vs. Ensembl Medaka
Match: unc13ba (protein unc-13 homolog B-like [Source:NCBI gene;Acc:101159330])

HSP 1 Score: 54.299 bits (129), Expect = 1.257e-9
Identity = 24/63 (38.10%), Postives = 40/63 (63.49%), Query Frame = -3

Query:  389 SQIVANISELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 577
             +I   + ++A+LL  ++G  N   A  E +++++LRPLME+ D +LS     CEKTV KR++
Sbjct:  834 GRINGCMRQMAELLYQMRGPPNRDTA--EADADVMLRPLMEFLDGNLSIFADACEKTVLKRIL 894

HSP 2 Score: 25.7942 bits (55), Expect = 1.257e-9
Identity = 10/21 (47.62%), Postives = 15/21 (71.43%), Query Frame = -2

Query:  309 ELWKITMHNLEKQVVLPPLSD 371
            +LWK  + +LEK +VLP  +D
Sbjct:  896 DLWKTVLCSLEKTIVLPQCND 916

[Close]

BLAST of SMED30025135 vs. Planmine SMEST
Match: SMESG000042667.1 (SMESG000042667.1)

HSP 1 Score: 124.405 bits (311), Expect = 2.152e-39
Identity = 61/62 (98.39%), Postives = 62/62 (100.00%), Query Frame = -3

Query:  389 QIVANISELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 574
            QIVANISELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRL+
Sbjct:  802 QIVANISELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLL 863

HSP 2 Score: 55.8398 bits (133), Expect = 2.152e-39
Identity = 25/28 (89.29%), Postives = 27/28 (96.43%), Query Frame = -2

Query:  288 ELWKITMHNLEKQVVLPPLSDPRQVRLN 371
            ELWKITMHNLEKQVVLPPLSDPRQ+ L+
Sbjct:  865 ELWKITMHNLEKQVVLPPLSDPRQLFLS 892

[Close]

BLAST of SMED30025135 vs. Planmine SMEST
Match: SMESG000042667.1 (SMESG000042667.1)

HSP 1 Score: 124.79 bits (312), Expect = 2.229e-39
Identity = 61/62 (98.39%), Postives = 62/62 (100.00%), Query Frame = -3

Query:  389 QIVANISELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 574
            QIVANISELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRL+
Sbjct: 1763 QIVANISELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLL 1824

HSP 2 Score: 55.4546 bits (132), Expect = 2.229e-39
Identity = 25/28 (89.29%), Postives = 27/28 (96.43%), Query Frame = -2

Query:  288 ELWKITMHNLEKQVVLPPLSDPRQVRLN 371
            ELWKITMHNLEKQVVLPPLSDPRQ+ L+
Sbjct: 1826 ELWKITMHNLEKQVVLPPLSDPRQLFLS 1853

[Close]

BLAST of SMED30025135 vs. Planmine SMEST
Match: SMESG000042667.1 (SMESG000042667.1)

HSP 1 Score: 124.79 bits (312), Expect = 2.401e-39
Identity = 61/62 (98.39%), Postives = 62/62 (100.00%), Query Frame = -3

Query:  389 QIVANISELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 574
            QIVANISELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRL+
Sbjct: 1725 QIVANISELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLL 1786

HSP 2 Score: 55.4546 bits (132), Expect = 2.401e-39
Identity = 25/28 (89.29%), Postives = 27/28 (96.43%), Query Frame = -2

Query:  288 ELWKITMHNLEKQVVLPPLSDPRQVRLN 371
            ELWKITMHNLEKQVVLPPLSDPRQ+ L+
Sbjct: 1788 ELWKITMHNLEKQVVLPPLSDPRQLFLS 1815

[Close]

BLAST of SMED30025135 vs. Planmine SMEST
Match: SMESG000042667.1 (SMESG000042667.1)

HSP 1 Score: 124.405 bits (311), Expect = 2.436e-39
Identity = 61/62 (98.39%), Postives = 62/62 (100.00%), Query Frame = -3

Query:  389 QIVANISELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 574
            QIVANISELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRL+
Sbjct:  802 QIVANISELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLL 863

HSP 2 Score: 55.8398 bits (133), Expect = 2.436e-39
Identity = 25/28 (89.29%), Postives = 27/28 (96.43%), Query Frame = -2

Query:  288 ELWKITMHNLEKQVVLPPLSDPRQVRLN 371
            ELWKITMHNLEKQVVLPPLSDPRQ+ L+
Sbjct:  865 ELWKITMHNLEKQVVLPPLSDPRQLFLS 892

[Close]

BLAST of SMED30025135 vs. Planmine SMEST
Match: SMESG000042667.1 (SMESG000042667.1)

HSP 1 Score: 124.79 bits (312), Expect = 2.462e-39
Identity = 61/62 (98.39%), Postives = 62/62 (100.00%), Query Frame = -3

Query:  389 QIVANISELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLI 574
            QIVANISELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRL+
Sbjct: 1719 QIVANISELAKLLQNLKGSQNNQKAHIEQESEIILRPLMEYFDASLSTLTSICEKTVFKRLL 1780

HSP 2 Score: 55.4546 bits (132), Expect = 2.462e-39
Identity = 25/28 (89.29%), Postives = 27/28 (96.43%), Query Frame = -2

Query:  288 ELWKITMHNLEKQVVLPPLSDPRQVRLN 371
            ELWKITMHNLEKQVVLPPLSDPRQ+ L+
Sbjct: 1782 ELWKITMHNLEKQVVLPPLSDPRQLFLS 1809

The following BLAST results are available for this feature:

BLAST of SMED30025135 vs. Ensembl Human
BLAST of SMED30025135 vs. Ensembl Celegans
BLAST of SMED30025135 vs. Ensembl Fly
BLAST of SMED30025135 vs. Ensembl Zebrafish
BLAST of SMED30025135 vs. Ensembl Xenopus
BLAST of SMED30025135 vs. Ensembl Mouse
BLAST of SMED30025135 vs. UniProt/SwissProt
BLAST of SMED30025135 vs. TrEMBL
BLAST of SMED30025135 vs. Ensembl Cavefish
BLAST of SMED30025135 vs. Ensembl Sea Lamprey
BLAST of SMED30025135 vs. Ensembl Yeast
BLAST of SMED30025135 vs. Ensembl Nematostella
BLAST of SMED30025135 vs. Ensembl Medaka
BLAST of SMED30025135 vs. Planmine SMEST

BLAST of SMED30025135 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
UNC13B	7.113e-11	33.33	unc-13 homolog B [Source:HGNC Symbol;Acc:HGNC:1256...	[more]
UNC13B	1.054e-10	33.33	unc-13 homolog B [Source:HGNC Symbol;Acc:HGNC:1256...	[more]
UNC13B	5.024e-10	33.33	unc-13 homolog B [Source:HGNC Symbol;Acc:HGNC:1256...	[more]
UNC13B	5.322e-10	33.33	unc-13 homolog B [Source:HGNC Symbol;Acc:HGNC:1256...	[more]
UNC13B	5.410e-10	33.33	unc-13 homolog B [Source:HGNC Symbol;Acc:HGNC:1256...	[more]

BLAST of SMED30025135 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
unc-13	1.622e-9	38.18	Phorbol ester/diacylglycerol-binding protein unc-1...	[more]
unc-13	1.622e-9	38.18	Phorbol ester/diacylglycerol-binding protein unc-1...	[more]
unc-13	1.834e-9	38.18	Phorbol ester/diacylglycerol-binding protein unc-1...	[more]
unc-13	1.849e-9	38.18	Phorbol ester/diacylglycerol-binding protein unc-1...	[more]
unc-13	1.880e-9	38.18	Phorbol ester/diacylglycerol-binding protein unc-1...	[more]

BLAST of SMED30025135 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
unc-13	1.374e-9	34.29	gene:FBgn0025726 transcript:FBtr0344971	[more]
unc-13	1.466e-9	34.29	gene:FBgn0025726 transcript:FBtr0089246	[more]
unc-13	1.466e-9	34.29	gene:FBgn0025726 transcript:FBtr0308805	[more]
unc-13	1.466e-9	34.29	gene:FBgn0025726 transcript:FBtr0089245	[more]
unc-13	1.618e-9	34.29	gene:FBgn0025726 transcript:FBtr0334487	[more]

BLAST of SMED30025135 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
CR769778.1	1.216e-11	36.84	protein unc-13 homolog C-like [Source:NCBI gene;Ac...	[more]
CR769778.1	1.216e-11	36.84	protein unc-13 homolog C-like [Source:NCBI gene;Ac...	[more]
UNC13B	4.984e-11	43.75	unc-13 homolog Bb (C. elegans) [Source:NCBI gene;A...	[more]
unc13a	9.783e-11	31.25	unc-13 homolog A (C. elegans) [Source:ZFIN;Acc:ZDB...	[more]
unc13a	1.176e-10	31.25	unc-13 homolog A (C. elegans) [Source:ZFIN;Acc:ZDB...	[more]

BLAST of SMED30025135 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
acaa1	2.020e-10	43.64	acetyl-CoA acyltransferase 1 [Source:Xenbase;Acc:X...	[more]
acaa1	2.039e-10	43.64	acetyl-CoA acyltransferase 1 [Source:Xenbase;Acc:X...	[more]
acaa1	2.332e-10	43.64	acetyl-CoA acyltransferase 1 [Source:Xenbase;Acc:X...	[more]
acaa1	2.457e-10	43.64	acetyl-CoA acyltransferase 1 [Source:Xenbase;Acc:X...	[more]
acaa1	2.616e-10	43.64	acetyl-CoA acyltransferase 1 [Source:Xenbase;Acc:X...	[more]

BLAST of SMED30025135 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Unc13b	9.809e-12	35.00	unc-13 homolog B [Source:MGI Symbol;Acc:MGI:134227...	[more]
Unc13b	1.565e-11	35.00	unc-13 homolog B [Source:MGI Symbol;Acc:MGI:134227...	[more]
Unc13b	6.455e-11	35.00	unc-13 homolog B [Source:MGI Symbol;Acc:MGI:134227...	[more]
Unc13b	6.560e-11	35.00	unc-13 homolog B [Source:MGI Symbol;Acc:MGI:134227...	[more]
Unc13b	7.057e-11	35.00	unc-13 homolog B [Source:MGI Symbol;Acc:MGI:134227...	[more]

BLAST of SMED30025135 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q9Z1N9\|UN13B_MOUSE	3.576e-10	35.00	Protein unc-13 homolog B OS=Mus musculus OX=10090 ...	[more]
sp\|Q62769\|UN13B_RAT	1.011e-9	35.00	Protein unc-13 homolog B OS=Rattus norvegicus OX=1...	[more]
sp\|Q8K0T7\|UN13C_MOUSE	2.239e-9	41.07	Protein unc-13 homolog C OS=Mus musculus OX=10090 ...	[more]
sp\|O14795\|UN13B_HUMAN	2.242e-9	33.33	Protein unc-13 homolog B OS=Homo sapiens OX=9606 G...	[more]
sp\|P27715\|UNC13_CAEEL	1.905e-8	38.18	Phorbol ester/diacylglycerol-binding protein unc-1...	[more]

BLAST of SMED30025135 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0L8G286	1.399e-16	50.00	Uncharacterized protein OS=Octopus bimaculoides OX...	[more]
A0A0L8G1T3	1.453e-16	50.00	Uncharacterized protein (Fragment) OS=Octopus bima...	[more]
A0A0L8G1T7	1.503e-16	50.00	Uncharacterized protein (Fragment) OS=Octopus bima...	[more]
A0A0L8G247	1.584e-16	50.00	Uncharacterized protein OS=Octopus bimaculoides OX...	[more]
A0A5K3F6A9	9.421e-16	50.00	Uncharacterized protein OS=Mesocestoides corti OX=...	[more]

BLAST of SMED30025135 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSAMXT00000045754.1	8.623e-11	37.50	pep primary_assembly:Astyanax_mexicanus-2.0:20:158...	[more]
ENSAMXT00000036882.1	1.602e-10	39.66	pep primary_assembly:Astyanax_mexicanus-2.0:20:158...	[more]
ENSAMXT00000049675.1	1.912e-10	39.66	pep primary_assembly:Astyanax_mexicanus-2.0:20:158...	[more]
ENSAMXT00000017969.2	2.525e-10	39.66	pep primary_assembly:Astyanax_mexicanus-2.0:20:157...	[more]
UNC13C	9.081e-10	36.84	unc-13 homolog C [Source:HGNC Symbol;Acc:HGNC:2314...	[more]

BLAST of SMED30025135 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30025135 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30025135 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO30233	1.226e-7	51.28	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30025135 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
ENSORLT00000005973.2	4.855e-11	33.33	unc-13 homolog A [Source:NCBI gene;Acc:101164968]	[more]
UNC13A	1.681e-10	35.48	protein unc-13 homolog A [Source:NCBI gene;Acc:105...	[more]
UNC13A	3.072e-10	35.48	protein unc-13 homolog A [Source:NCBI gene;Acc:105...	[more]
UNC13B	4.315e-10	39.66	unc-13 homolog B [Source:NCBI gene;Acc:101167071]	[more]
unc13ba	1.257e-9	38.10	protein unc-13 homolog B-like [Source:NCBI gene;Ac...	[more]

BLAST of SMED30025135 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000042667.1	2.152e-39	98.39	SMESG000042667.1	[more]
SMESG000042667.1	2.229e-39	98.39	SMESG000042667.1	[more]
SMESG000042667.1	2.401e-39	98.39	SMESG000042667.1	[more]
SMESG000042667.1	2.436e-39	98.39	SMESG000042667.1	[more]
SMESG000042667.1	2.462e-39	98.39	SMESG000042667.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30025135 ID=SMED30025135|Name=SMED30025135|organism=Schmidtea mediterranea sexual|type=transcript|length=585bp
TTTAATTATTGTCAGTTAATTAATTTATTTATGTTCAAATTAAGCATTTG
GAAAACAAAGGAAAAAACTCCAATTCTGTTCTGTTCCAACGCAATATGCG
AACAAAGTACACAGAAATTACGATCCAAAAAAAGTAGAATAATGAATATT
TTCGCAGAGAAATTCATGTGTTTGGATAATGCTAATAAATGCCGAGTTCC
AGCACTTGGACTTCGATTCCTCACTGACAATCAATGCAACTCCAAAAATT
TTCAAAACAACAGAATTAAAAAAAACATTTATTTACAATTTAATCTTACT
TGTCTTGGATCACTTAAAGGTGGAAGAACAACTTGCTTTTCTAAATTATG
CATTGTAATTTTCCATAATTCCTAAAAGTTCAGAATTAAATTAATCGCTT
GAAAACCGTTTTCTCACAAATGGAGGTCAACGTAGATAAACTCGCATCGA
AATACTCCATTAATGGCCGAAGGATAATTTCCGACTCTTGTTCAATATGA
GCTTTCTGATTATTTTGGCTGCCTTTAAGATTCTGTAATAATTTTGCTAA
TTCAGAGATGTTGGCTACAATCTGAGAGATCGGAA

protein sequence of SMED30025135-orf-1

>SMED30025135-orf-1 ID=SMED30025135-orf-1|Name=SMED30025135-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=125bp
FNYCQLINLFMFKLSIWKTKEKTPILFCSNAICEQSTQKLRSKKSRIMNI
FAEKFMCLDNANKCRVPALGLRFLTDNQCNSKNFQNNRIKKNIYLQFNLT
CLGSLKGGRTTCFSKLCIVIFHNS*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000099	neuron
PLANA:0000418	head

Vocabulary: **INTERPRO**
Term	Definition
IPR014772	Munc13_dom-2

Main menu

SMED30025135

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 13

Owlery

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