SMED30025129

Overview
NameSMED30025129
Smed IDSMED30025129
Length (bp)1019
Neoblast Clusters

Zeng et. al., 2018




 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30025129

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
neuronSMED30025129SMESG000004295.1 dd_Smed_v4_29022_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
headSMED30025129SMESG000004295.1 dd_Smed_v6_29022_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30025129 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000126:166014..168461 -4919
Homology
BLAST of SMED30025129 vs. Planmine SMEST
Match: SMESG000004295.1 (SMESG000004295.1)

HSP 1 Score: 605.134 bits (1559), Expect = 0.000e+0
Identity = 319/322 (99.07%), Postives = 320/322 (99.38%), Query Frame = 1
Query:    1 DYNKNEKKFNKNQTIYFEFVKIINPIFQIXXXXXXXXXXFKTFILKLCLDESSVKYSEKIAIIIGFLRQLRSKQFITIELEDFQQELLTTYATFLKNETEEDFQIEDLLNHLIKLGEIFIGIGCGDSSTIIIQPLKYIEAVNILYSNPDNQTLKIESRRFWMKSDNRPNCTSLASMIERAFENNTFIDCILPILLQNLNLSNDRLYMLCRLMLSEAVLIMADLPHLNVVNEEIAMPYYPSFSKGVTYVKPNKANLERKESVMKWFSSEKVSSLSILYKFSQTVDLQMLMPLIYFIGGKIGTEEEKVKWFKFGIFYIINQVGL 966
            DYNKNEKKFNKNQTIYFEFVKIINPIFQIDMDSDKDMKDFKTFILKLCLDESSVKYSEKIAIIIGFLRQLRSKQFITIELEDFQQELLTTYATFLKNETEEDFQIEDLLNHLIKLGEIFIGIGCGDSSTIIIQPLKYIEAVNILYSNPDNQTLKIESRRFWMKSDNRPNCTSLASMIERAFENNTFIDCILPILLQNLNLSNDRLYMLCRLMLSEAVLIMADLPHLNVVNEEIAMPYYPSFSKGVTYVKPNKANLERKESVMKWFSSEKVSSLSILYKFSQTVDLQMLMPLIYFIGGKIGTEEEKVKWFKFGIFYIINQ  +
Sbjct:  202 DYNKNEKKFNKNQTIYFEFVKIINPIFQIDMDSDKDMKDFKTFILKLCLDESSVKYSEKIAIIIGFLRQLRSKQFITIELEDFQQELLTTYATFLKNETEEDFQIEDLLNHLIKLGEIFIGIGCGDSSTIIIQPLKYIEAVNILYSNPDNQTLKIESRRFWMKSDNRPNCTSLASMIERAFENNTFIDCILPILLQNLNLSNDRLYMLCRLMLSEAVLIMADLPHLNVVNEEIAMPYYPSFSKGVTYVKPNKANLERKESVMKWFSSEKVSSLSILYKFSQTVDLQMLMPLIYFIGGKIGTEEEKVKWFKFGIFYIINQFSI 523          
BLAST of SMED30025129 vs. Planmine SMEST
Match: SMESG000004295.1 (SMESG000004295.1)

HSP 1 Score: 577.015 bits (1486), Expect = 0.000e+0
Identity = 280/283 (98.94%), Postives = 281/283 (99.29%), Query Frame = 1
Query:  118 FKTFILKLCLDESSVKYSEKIAIIIGFLRQLRSKQFITIELEDFQQELLTTYATFLKNETEEDFQIEDLLNHLIKLGEIFIGIGCGDSSTIIIQPLKYIEAVNILYSNPDNQTLKIESRRFWMKSDNRPNCTSLASMIERAFENNTFIDCILPILLQNLNLSNDRLYMLCRLMLSEAVLIMADLPHLNVVNEEIAMPYYPSFSKGVTYVKPNKANLERKESVMKWFSSEKVSSLSILYKFSQTVDLQMLMPLIYFIGGKIGTEEEKVKWFKFGIFYIINQVGL 966
            FKTFILKLCLDESSVKYSEKIAIIIGFLRQLRSKQFITIELEDFQQELLTTYATFLKNETEEDFQIEDLLNHLIKLGEIFIGIGCGDSSTIIIQPLKYIEAVNILYSNPDNQTLKIESRRFWMKSDNRPNCTSLASMIERAFENNTFIDCILPILLQNLNLSNDRLYMLCRLMLSEAVLIMADLPHLNVVNEEIAMPYYPSFSKGVTYVKPNKANLERKESVMKWFSSEKVSSLSILYKFSQTVDLQMLMPLIYFIGGKIGTEEEKVKWFKFGIFYIINQ  +
Sbjct:    4 FKTFILKLCLDESSVKYSEKIAIIIGFLRQLRSKQFITIELEDFQQELLTTYATFLKNETEEDFQIEDLLNHLIKLGEIFIGIGCGDSSTIIIQPLKYIEAVNILYSNPDNQTLKIESRRFWMKSDNRPNCTSLASMIERAFENNTFIDCILPILLQNLNLSNDRLYMLCRLMLSEAVLIMADLPHLNVVNEEIAMPYYPSFSKGVTYVKPNKANLERKESVMKWFSSEKVSSLSILYKFSQTVDLQMLMPLIYFIGGKIGTEEEKVKWFKFGIFYIINQFSI 286          
BLAST of SMED30025129 vs. Planmine SMEST
Match: SMESG000004295.1 (SMESG000004295.1)

HSP 1 Score: 454.907 bits (1169), Expect = 1.247e-158
Identity = 221/224 (98.66%), Postives = 222/224 (99.11%), Query Frame = 1
Query:  118 FKTFILKLCLDESSVKYSEKIAIIIGFLRQLRSKQFITIELEDFQQELLTTYATFLKNETEEDFQIEDLLNHLIKLGEIFIGIGCGDSSTIIIQPLKYIEAVNILYSNPDNQTLKIESRRFWMKSDNRPNCTSLASMIERAFENNTFIDCILPILLQNLNLSNDRLYMLCRLMLSEAVLIMADLPHLNVVNEEIAMPYYPSFSKGVTYVKPNKANLERKESVMK 789
            FKTFILKLCLDESSVKYSEKIAIIIGFLRQLRSKQFITIELEDFQQELLTTYATFLKNETEEDFQIEDLLNHLIKLGEIFIGIGCGDSSTIIIQPLKYIEAVNILYSNPDNQTLKIESRRFWMKSDNRPNCTSLASMIERAFENNTFIDCILPILLQNLNLSNDRLYMLCRLMLSEAVLIMADLPHLNVVNEEIAMPYYPSFSKGVTYVKPNKANLERKE  +K
Sbjct:    4 FKTFILKLCLDESSVKYSEKIAIIIGFLRQLRSKQFITIELEDFQQELLTTYATFLKNETEEDFQIEDLLNHLIKLGEIFIGIGCGDSSTIIIQPLKYIEAVNILYSNPDNQTLKIESRRFWMKSDNRPNCTSLASMIERAFENNTFIDCILPILLQNLNLSNDRLYMLCRLMLSEAVLIMADLPHLNVVNEEIAMPYYPSFSKGVTYVKPNKANLERKEFSIK 227          
The following BLAST results are available for this feature:
BLAST of SMED30025129 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025129 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025129 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025129 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025129 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025129 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025129 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025129 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025129 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025129 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025129 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025129 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025129 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025129 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000004295.10.000e+099.07SMESG000004295.1[more]
SMESG000004295.10.000e+098.94SMESG000004295.1[more]
SMESG000004295.11.247e-15898.66SMESG000004295.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30025129 ID=SMED30025129|Name=SMED30025129|organism=Schmidtea mediterranea sexual|type=transcript|length=1019bp
GATTACAATAAAAATGAAAAGAAATTTAATAAGAATCAAACTATTTATTT
TGAATTCGTTAAAATAATTAATCCTATATTTCAAATTGATATGGATTCAG
ATAAGGATATGAAAGATTTTAAGACATTTATTCTGAAATTATGCCTGGAT
GAGAGCTCAGTCAAATATTCAGAAAAGATAGCTATTATCATTGGTTTTCT
CCGCCAGTTGAGATCTAAACAGTTTATAACAATAGAACTAGAAGATTTTC
AGCAAGAACTATTAACAACTTATGCAACATTTTTAAAAAATGAAACAGAA
GAAGATTTTCAAATCGAAGACCTTTTAAATCATTTGATAAAGTTGGGTGA
AATATTTATCGGAATTGGTTGTGGTGATTCTTCGACAATAATCATCCAAC
CTCTCAAATATATAGAAGCAGTTAATATACTATATTCTAATCCTGATAAC
CAAACACTTAAGATAGAATCAAGAAGATTCTGGATGAAATCTGATAATAG
ACCGAATTGCACATCATTGGCAAGCATGATAGAAAGAGCATTCGAAAATA
ACACTTTTATTGACTGTATATTACCCATTCTCCTTCAGAATTTGAATTTA
TCAAATGACAGATTGTATATGCTTTGCAGATTGATGTTAAGTGAAGCTGT
ACTAATTATGGCAGATCTTCCCCATTTGAATGTAGTCAATGAAGAAATCG
CAATGCCATATTATCCATCATTTTCAAAAGGTGTTACATATGTAAAACCA
AATAAAGCTAATTTAGAAAGGAAAGAATCTGTAATGAAATGGTTTAGTTC
CGAAAAAGTTTCCAGTTTATCTATATTGTATAAATTTTCTCAAACTGTTG
ATTTACAAATGTTAATGCCATTAATTTATTTTATTGGAGGCAAAATTGGA
ACCGAGGAAGAAAAAGTTAAATGGTTTAAATTCGGAATATTTTACATTAT
TAACCAAGTGGGATTATACTTTTTGACATTTTCATGATACTGCATATTTG
GGGTCGGCAATATTACAGA
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protein sequence of SMED30025129-orf-1

>SMED30025129-orf-1 ID=SMED30025129-orf-1|Name=SMED30025129-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=329bp
DYNKNEKKFNKNQTIYFEFVKIINPIFQIDMDSDKDMKDFKTFILKLCLD
ESSVKYSEKIAIIIGFLRQLRSKQFITIELEDFQQELLTTYATFLKNETE
EDFQIEDLLNHLIKLGEIFIGIGCGDSSTIIIQPLKYIEAVNILYSNPDN
QTLKIESRRFWMKSDNRPNCTSLASMIERAFENNTFIDCILPILLQNLNL
SNDRLYMLCRLMLSEAVLIMADLPHLNVVNEEIAMPYYPSFSKGVTYVKP
NKANLERKESVMKWFSSEKVSSLSILYKFSQTVDLQMLMPLIYFIGGKIG
TEEEKVKWFKFGIFYIINQVGLYFLTFS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head