Eukaryotic initiation factor-4G
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30025106 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Human
Match: EIF4G3 (eukaryotic translation initiation factor 4 gamma 3 [Source:HGNC Symbol;Acc:HGNC:3298]) HSP 1 Score: 147.902 bits (372), Expect = 5.360e-35 Identity = 126/418 (30.14%), Postives = 211/418 (50.48%), Query Frame = 2
Query: 1268 YDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQPRVISCQLPPE--SSSSQTNNERANNKKQLPNRQYSKYKNYNHKQTE-------RKQINLEISDNQVFIRSKSGYLPPQLRDKQKLLDSLE-----ELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFC---YKLAITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDS-KMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKF-NLMTKKKNVSNSQNI 2449
YDR +L+ + QKP +P ++ D I NQP++ L P A+ +Q P + N ++++ RK I + + ++ ++++ + P Q RD Q D E EL R ++S LNK+TPQ F + +K++ L ++ +E + +I+ +F KA+ E ++SV YA C L + + P F+ LL +CQ FE +D+ ++ E K+K L + + + +LEE KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K LMTK+K Q +
Sbjct: 344 YDREFLLDFQFMPACIQKPEGLPPISDVVLDKI-NQPKLPMRTLDPRILPRGPDFTPAFADFGRQTPGGRGVPLLNVGSRRSQPGQRREPRKIITVSVKEDVHLKKAENAWKPSQKRDSQA--DDPENIKTQELFRKVRSILNKLTPQMFNQLMKQVSGLTVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLVTLKVPMADKPGNTVNFRKLLLNRCQKEFE---KDKADDDVFEKKQKELEAASAPEERTRLHDELEEAKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLCNWVSRRADQ----GPKTIEQIHKEAKIEEQEEQRKVQQLMTKEKRRPGVQRV 750 HSP 2 Score: 65.855 bits (159), Expect = 6.069e-10 Identity = 68/290 (23.45%), Postives = 130/290 (44.83%), Query Frame = 2
Query: 2918 VDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFE---NIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNHWET--FLKVNNLEFTVDLPGLSLCGGDPLN---FIAEELEDML-----SNNLSNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSV----QEYLKSKKEIIKRISNHKLENEIEILKSILNVASELNRDT--LTRIYDLLITEKIVSHQAL 3727
V+ +E+ R +G L +L+ + +S Q +G + DD+ DIP IW + A+ + + K IS E K +LP + S LH ++ HK VG ++ ++ L W+ +P + FL L+F + S C + L+ AEEL L + ++E+IF+++E ++D+ + + +++ KA I +SS +K + I+ + + E E++ L ++ +L++ L +D L E+++S A
Sbjct: 983 VESTLERSQITRDHMGQLLYQLVQSEKLSKQDFFKGFSETLELADDMAIDIPHIWLYLAELVTPML--KEGGISMRELTIEFSKPLLPVGRAGVLLSEILHLLCKQMSHKKVGALWREADLSWKDFLPEGEDVHNFLLEQKLDF---IESDSPCSSEALSKKELSAEELYKRLEKLIIEDKANDEQIFDWVEANLDEIQMSSPTFLRALMTAVCKAA-IIADSSTFRVDTAVIKQRVPILLKYLDSDTEKELQALYALQASIVKLDQPANLLRMFFDCLYDEEVISEDAF 1266
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Human
Match: EIF4G3 (eukaryotic translation initiation factor 4 gamma 3 [Source:HGNC Symbol;Acc:HGNC:3298]) HSP 1 Score: 147.902 bits (372), Expect = 6.030e-35 Identity = 126/418 (30.14%), Postives = 210/418 (50.24%), Query Frame = 2
Query: 1268 YDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQPRVISCQLPPE--SSSSQTNNERANNKKQLPNRQY-------SKYKNYNHKQTERKQINLEISDNQVFIRSKSGYLPPQLRDKQKLLDSLE-----ELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFC---YKLAITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDS-KMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKF-NLMTKKKNVSNSQNI 2449
YDR +L+ + QKP +P ++ D I NQP++ L P A+ +Q P + S+ ++ RK I + + ++ ++++ + P Q RD Q D E EL R ++S LNK+TPQ F + +K++ L ++ +E + +I+ +F KA+ E ++SV YA C L + + P F+ LL +CQ FE +D+ ++ E K+K L + + + +LEE KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K LMTK+K Q +
Sbjct: 630 YDREFLLDFQFMPACIQKPEGLPPISDVVLDKI-NQPKLPMRTLDPRILPRGPDFTPAFADFGRQTPGGRGVPLLNVGSRRSQPGQRREPRKIITVSVKEDVHLKKAENAWKPSQKRDSQA--DDPENIKTQELFRKVRSILNKLTPQMFNQLMKQVSGLTVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLVTLKVPMADKPGNTVNFRKLLLNRCQKEFE---KDKADDDVFEKKQKELEAASAPEERTRLHDELEEAKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLCNWVSRRADQ----GPKTIEQIHKEAKIEEQEEQRKVQQLMTKEKRRPGVQRV 1036 HSP 2 Score: 63.929 bits (154), Expect = 2.759e-9 Identity = 68/290 (23.45%), Postives = 130/290 (44.83%), Query Frame = 2
Query: 2918 VDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFE---NIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNHWET--FLKVNNLEFTVDLPGLSLCGGDPLN---FIAEELEDML-----SNNLSNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSV----QEYLKSKKEIIKRISNHKLENEIEILKSILNVASELNRDT--LTRIYDLLITEKIVSHQAL 3727
V+ +E+ R +G L +L+ + +S Q +G + DD+ DIP IW + A+ + + K IS E K +LP + S LH ++ HK VG ++ ++ L W+ +P + FL L+F + S C + L+ AEEL L + ++E+IF+++E ++D+ + + +++ KA I +SS +K + I+ + + E E++ L ++ +L++ L +D L E+++S A
Sbjct: 1269 VESTLERSQITRDHMGQLLYQLVQSEKLSKQDFFKGFSETLELADDMAIDIPHIWLYLAELVTPML--KEGGISMRELTIEFSKPLLPVGRAGVLLSEILHLLCKQMSHKKVGALWREADLSWKDFLPEGEDVHNFLLEQKLDF---IESDSPCSSEALSKKELSAEELYKRLEKLIIEDKANDEQIFDWVEANLDEIQMSSPTFLRALMTAVCKAA-IIADSSTFRVDTAVIKQRVPILLKYLDSDTEKELQALYALQASIVKLDQPANLLRMFFDCLYDEEVISEDAF 1552
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Human
Match: EIF4G3 (eukaryotic translation initiation factor 4 gamma 3 [Source:HGNC Symbol;Acc:HGNC:3298]) HSP 1 Score: 147.902 bits (372), Expect = 6.221e-35 Identity = 126/418 (30.14%), Postives = 210/418 (50.24%), Query Frame = 2
Query: 1268 YDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQPRVISCQLPPE--SSSSQTNNERANNKKQLPNRQY-------SKYKNYNHKQTERKQINLEISDNQVFIRSKSGYLPPQLRDKQKLLDSLE-----ELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFC---YKLAITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDS-KMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKF-NLMTKKKNVSNSQNI 2449
YDR +L+ + QKP +P ++ D I NQP++ L P A+ +Q P + S+ ++ RK I + + ++ ++++ + P Q RD Q D E EL R ++S LNK+TPQ F + +K++ L ++ +E + +I+ +F KA+ E ++SV YA C L + + P F+ LL +CQ FE +D+ ++ E K+K L + + + +LEE KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K LMTK+K Q +
Sbjct: 624 YDREFLLDFQFMPACIQKPEGLPPISDVVLDKI-NQPKLPMRTLDPRILPRGPDFTPAFADFGRQTPGGRGVPLLNVGSRRSQPGQRREPRKIITVSVKEDVHLKKAENAWKPSQKRDSQA--DDPENIKTQELFRKVRSILNKLTPQMFNQLMKQVSGLTVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLVTLKVPMADKPGNTVNFRKLLLNRCQKEFE---KDKADDDVFEKKQKELEAASAPEERTRLHDELEEAKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLCNWVSRRADQ----GPKTIEQIHKEAKIEEQEEQRKVQQLMTKEKRRPGVQRV 1030 HSP 2 Score: 63.929 bits (154), Expect = 2.711e-9 Identity = 68/290 (23.45%), Postives = 130/290 (44.83%), Query Frame = 2
Query: 2918 VDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFE---NIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNHWET--FLKVNNLEFTVDLPGLSLCGGDPLN---FIAEELEDML-----SNNLSNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSV----QEYLKSKKEIIKRISNHKLENEIEILKSILNVASELNRDT--LTRIYDLLITEKIVSHQAL 3727
V+ +E+ R +G L +L+ + +S Q +G + DD+ DIP IW + A+ + + K IS E K +LP + S LH ++ HK VG ++ ++ L W+ +P + FL L+F + S C + L+ AEEL L + ++E+IF+++E ++D+ + + +++ KA I +SS +K + I+ + + E E++ L ++ +L++ L +D L E+++S A
Sbjct: 1263 VESTLERSQITRDHMGQLLYQLVQSEKLSKQDFFKGFSETLELADDMAIDIPHIWLYLAELVTPML--KEGGISMRELTIEFSKPLLPVGRAGVLLSEILHLLCKQMSHKKVGALWREADLSWKDFLPEGEDVHNFLLEQKLDF---IESDSPCSSEALSKKELSAEELYKRLEKLIIEDKANDEQIFDWVEANLDEIQMSSPTFLRALMTAVCKAA-IIADSSTFRVDTAVIKQRVPILLKYLDSDTEKELQALYALQASIVKLDQPANLLRMFFDCLYDEEVISEDAF 1546
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Human
Match: EIF4G3 (eukaryotic translation initiation factor 4 gamma 3 [Source:HGNC Symbol;Acc:HGNC:3298]) HSP 1 Score: 147.517 bits (371), Expect = 7.797e-35 Identity = 126/418 (30.14%), Postives = 210/418 (50.24%), Query Frame = 2
Query: 1268 YDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQPRVISCQLPPE--SSSSQTNNERANNKKQLPNRQY-------SKYKNYNHKQTERKQINLEISDNQVFIRSKSGYLPPQLRDKQKLLDSLE-----ELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFC---YKLAITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDS-KMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKF-NLMTKKKNVSNSQNI 2449
YDR +L+ + QKP +P ++ D I NQP++ L P A+ +Q P + S+ ++ RK I + + ++ ++++ + P Q RD Q D E EL R ++S LNK+TPQ F + +K++ L ++ +E + +I+ +F KA+ E ++SV YA C L + + P F+ LL +CQ FE +D+ ++ E K+K L + + + +LEE KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K LMTK+K Q +
Sbjct: 813 YDREFLLDFQFMPACIQKPEGLPPISDVVLDKI-NQPKLPMRTLDPRILPRGPDFTPAFADFGRQTPGGRGVPLLNVGSRRSQPGQRREPRKIITVSVKEDVHLKKAENAWKPSQKRDSQA--DDPENIKTQELFRKVRSILNKLTPQMFNQLMKQVSGLTVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLVTLKVPMADKPGNTVNFRKLLLNRCQKEFE---KDKADDDVFEKKQKELEAASAPEERTRLHDELEEAKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLCNWVSRRADQ----GPKTIEQIHKEAKIEEQEEQRKVQQLMTKEKRRPGVQRV 1219 HSP 2 Score: 63.5438 bits (153), Expect = 3.505e-9 Identity = 68/290 (23.45%), Postives = 130/290 (44.83%), Query Frame = 2
Query: 2918 VDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFE---NIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNHWET--FLKVNNLEFTVDLPGLSLCGGDPLN---FIAEELEDML-----SNNLSNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSV----QEYLKSKKEIIKRISNHKLENEIEILKSILNVASELNRDT--LTRIYDLLITEKIVSHQAL 3727
V+ +E+ R +G L +L+ + +S Q +G + DD+ DIP IW + A+ + + K IS E K +LP + S LH ++ HK VG ++ ++ L W+ +P + FL L+F + S C + L+ AEEL L + ++E+IF+++E ++D+ + + +++ KA I +SS +K + I+ + + E E++ L ++ +L++ L +D L E+++S A
Sbjct: 1452 VESTLERSQITRDHMGQLLYQLVQSEKLSKQDFFKGFSETLELADDMAIDIPHIWLYLAELVTPML--KEGGISMRELTIEFSKPLLPVGRAGVLLSEILHLLCKQMSHKKVGALWREADLSWKDFLPEGEDVHNFLLEQKLDF---IESDSPCSSEALSKKELSAEELYKRLEKLIIEDKANDEQIFDWVEANLDEIQMSSPTFLRALMTAVCKAA-IIADSSTFRVDTAVIKQRVPILLKYLDSDTEKELQALYALQASIVKLDQPANLLRMFFDCLYDEEVISEDAF 1735
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Human
Match: EIF4G3 (eukaryotic translation initiation factor 4 gamma 3 [Source:HGNC Symbol;Acc:HGNC:3298]) HSP 1 Score: 139.043 bits (349), Expect = 2.829e-32 Identity = 108/340 (31.76%), Postives = 181/340 (53.24%), Query Frame = 2
Query: 1475 SKYKNYNHKQTERKQINLEISDNQVFIRSKSGYLPPQLRDKQKLLDSLE-----ELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFC---YKLAITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDS-KMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKF-NLMTKKKNVSNSQNI 2449
S+ ++ RK I + + ++ ++++ + P Q RD Q D E EL R ++S LNK+TPQ F + +K++ L ++ +E + +I+ +F KA+ E ++SV YA C L + + P F+ LL +CQ FE +D+ ++ E K+K L + + + +LEE KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K LMTK+K Q +
Sbjct: 737 SRRSQPGQRREPRKIITVSVKEDVHLKKAENAWKPSQKRDSQA--DDPENIKTQELFRKVRSILNKLTPQMFNQLMKQVSGLTVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLVTLKVPMADKPGNTVNFRKLLLNRCQKEFE---KDKADDDVFEKKQKELEAASAPEERTRLHDELEEAKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLCNWVSRRADQ----GPKTIEQIHKEAKIEEQEEQRKVQQLMTKEKRRPGVQRV 1066 HSP 2 Score: 63.929 bits (154), Expect = 2.723e-9 Identity = 67/290 (23.10%), Postives = 131/290 (45.17%), Query Frame = 2
Query: 2918 VDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFE---NIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNHWET--FLKVNNLEFTVDLPGLSLCGGDPLN---FIAEELED-----MLSNNLSNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSV----QEYLKSKKEIIKRISNHKLENEIEILKSILNVASELNRDT--LTRIYDLLITEKIVSHQAL 3727
V+ +E+ R +G L +L+ + +S Q +G + DD+ DIP IW + A+ + + K IS E K +LP + S LH ++ HK VG ++ ++ L W+ +P + FL L+F + S C + L+ AEEL ++ + ++E+IF+++E ++D+ + + +++ KA I +SS +K + I+ + + E E++ L ++ +L++ L +D L E+++S A
Sbjct: 1299 VESTLERSQITRDHMGQLLYQLVQSEKLSKQDFFKGFSETLELADDMAIDIPHIWLYLAELVTPML--KEGGISMRELTIEFSKPLLPVGRAGVLLSEILHLLCKQMSHKKVGALWREADLSWKDFLPEGEDVHNFLLEQKLDF---IESDSPCSSEALSKKELSAEELYKRLEKLIIEDKANDEQIFDWVEANLDEIQMSSPTFLRALMTAVCKAA-IIADSSTFRVDTAVIKQRVPILLKYLDSDTEKELQALYALQASIVKLDQPANLLRMFFDCLYDEEVISEDAF 1582
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Celegans
Match: ifg-1 (Initiation Factor 4G (eIF4G) family [Source:UniProtKB/TrEMBL;Acc:B5BM23]) HSP 1 Score: 59.6918 bits (143), Expect = 1.634e-8 Identity = 61/249 (24.50%), Postives = 123/249 (49.40%), Query Frame = 2
Query: 1622 ELKRNIKSNLNKITPQN-FPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA---ITYTNN-PAFKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELL---------RDSYDSDAMLALCKLLETCGKQIDVKHKESMNKYISDISRLSQSVDLEK-----RIRFKLKDIIDMRERNWTLR 2311
E+ + ++S +NK+TP + P + I N +++ ++ +F KAV+E + YA+ C A ++ T AF+ ++L + Q F QD+ +++ + + E D K L L E+ +K + + FG + F+G L+ + LL+ K++ C EL ++ D +++ +L+ET G +D K K+S + + + Q ++ K +IRF + +++++R+ W R
Sbjct: 402 EVCKKVRSLMNKVTPTSQRPLTEEFISYNVSSNDAQLAQVVEIVFDKAVEEPKFCALYAEMCKAQANHELSQTGGKSAFRNKVLTRTQMTF----QDK-KDIDADKLAIIEKEEDPVKKELM---LAEEKQKFRRRKFGVMTFMGYLYRNQLLSTKIVQTCTFELFNSIKDQDIKKEDVDEESIHCGLQLIETVGVMLD-KSKDSTTVF---LDQWFQKLEAAKPYCSNKIRFMIMNLMELRKDKWIPR 638
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Celegans
Match: ifg-1 (Initiation Factor 4G (eIF4G) family [Source:UniProtKB/TrEMBL;Acc:B5BM23]) HSP 1 Score: 59.6918 bits (143), Expect = 1.634e-8 Identity = 61/249 (24.50%), Postives = 123/249 (49.40%), Query Frame = 2
Query: 1622 ELKRNIKSNLNKITPQN-FPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA---ITYTNN-PAFKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELL---------RDSYDSDAMLALCKLLETCGKQIDVKHKESMNKYISDISRLSQSVDLEK-----RIRFKLKDIIDMRERNWTLR 2311
E+ + ++S +NK+TP + P + I N +++ ++ +F KAV+E + YA+ C A ++ T AF+ ++L + Q F QD+ +++ + + E D K L L E+ +K + + FG + F+G L+ + LL+ K++ C EL ++ D +++ +L+ET G +D K K+S + + + Q ++ K +IRF + +++++R+ W R
Sbjct: 402 EVCKKVRSLMNKVTPTSQRPLTEEFISYNVSSNDAQLAQVVEIVFDKAVEEPKFCALYAEMCKAQANHELSQTGGKSAFRNKVLTRTQMTF----QDK-KDIDADKLAIIEKEEDPVKKELM---LAEEKQKFRRRKFGVMTFMGYLYRNQLLSTKIVQTCTFELFNSIKDQDIKKEDVDEESIHCGLQLIETVGVMLD-KSKDSTTVF---LDQWFQKLEAAKPYCSNKIRFMIMNLMELRKDKWIPR 638
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Celegans
Match: ifg-1 (Initiation Factor 4G (eIF4G) family [Source:UniProtKB/TrEMBL;Acc:B5BM23]) HSP 1 Score: 59.6918 bits (143), Expect = 1.634e-8 Identity = 61/249 (24.50%), Postives = 123/249 (49.40%), Query Frame = 2
Query: 1622 ELKRNIKSNLNKITPQN-FPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA---ITYTNN-PAFKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELL---------RDSYDSDAMLALCKLLETCGKQIDVKHKESMNKYISDISRLSQSVDLEK-----RIRFKLKDIIDMRERNWTLR 2311
E+ + ++S +NK+TP + P + I N +++ ++ +F KAV+E + YA+ C A ++ T AF+ ++L + Q F QD+ +++ + + E D K L L E+ +K + + FG + F+G L+ + LL+ K++ C EL ++ D +++ +L+ET G +D K K+S + + + Q ++ K +IRF + +++++R+ W R
Sbjct: 402 EVCKKVRSLMNKVTPTSQRPLTEEFISYNVSSNDAQLAQVVEIVFDKAVEEPKFCALYAEMCKAQANHELSQTGGKSAFRNKVLTRTQMTF----QDK-KDIDADKLAIIEKEEDPVKKELM---LAEEKQKFRRRKFGVMTFMGYLYRNQLLSTKIVQTCTFELFNSIKDQDIKKEDVDEESIHCGLQLIETVGVMLD-KSKDSTTVF---LDQWFQKLEAAKPYCSNKIRFMIMNLMELRKDKWIPR 638
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Celegans
Match: ifg-1 (Initiation Factor 4G (eIF4G) family [Source:UniProtKB/TrEMBL;Acc:B5BM23]) HSP 1 Score: 59.6918 bits (143), Expect = 1.786e-8 Identity = 61/249 (24.50%), Postives = 123/249 (49.40%), Query Frame = 2
Query: 1622 ELKRNIKSNLNKITPQN-FPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA---ITYTNN-PAFKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELL---------RDSYDSDAMLALCKLLETCGKQIDVKHKESMNKYISDISRLSQSVDLEK-----RIRFKLKDIIDMRERNWTLR 2311
E+ + ++S +NK+TP + P + I N +++ ++ +F KAV+E + YA+ C A ++ T AF+ ++L + Q F QD+ +++ + + E D K L L E+ +K + + FG + F+G L+ + LL+ K++ C EL ++ D +++ +L+ET G +D K K+S + + + Q ++ K +IRF + +++++R+ W R
Sbjct: 516 EVCKKVRSLMNKVTPTSQRPLTEEFISYNVSSNDAQLAQVVEIVFDKAVEEPKFCALYAEMCKAQANHELSQTGGKSAFRNKVLTRTQMTF----QDK-KDIDADKLAIIEKEEDPVKKELM---LAEEKQKFRRRKFGVMTFMGYLYRNQLLSTKIVQTCTFELFNSIKDQDIKKEDVDEESIHCGLQLIETVGVMLD-KSKDSTTVF---LDQWFQKLEAAKPYCSNKIRFMIMNLMELRKDKWIPR 752
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Celegans
Match: ifg-1 (Initiation Factor 4G (eIF4G) family [Source:UniProtKB/TrEMBL;Acc:B5BM23]) HSP 1 Score: 59.6918 bits (143), Expect = 1.926e-8 Identity = 61/250 (24.40%), Postives = 125/250 (50.00%), Query Frame = 2
Query: 1622 ELKRNIKSNLNKITPQN-FPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFC-----YKLAITYTNNPAFKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELL---------RDSYDSDAMLALCKLLETCGKQIDVKHKESMNKYISDISRLSQSVDLEK-----RIRFKLKDIIDMRERNWTLR 2311
E+ + ++S +NK+TP + P + I N +++ ++ +F KAV+E + YA+ C ++L+ T + AF+ ++L + Q F QD+ +++ + + E D K L L E+ +K + + FG + F+G L+ + LL+ K++ C EL ++ D +++ +L+ET G +D K K+S + + + Q ++ K +IRF + +++++R+ W R
Sbjct: 515 EVCKKVRSLMNKVTPTSQRPLTEEFISYNVSSNDAQLAQVVEIVFDKAVEEPKFCALYAEMCKAQANHELSQTGGKS-AFRNKVLTRTQMTF----QDK-KDIDADKLAIIEKEEDPVKKELM---LAEEKQKFRRRKFGVMTFMGYLYRNQLLSTKIVQTCTFELFNSIKDQDIKKEDVDEESIHCGLQLIETVGVMLD-KSKDSTTVF---LDQWFQKLEAAKPYCSNKIRFMIMNLMELRKDKWIPR 751
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Fly
Match: NAT1 (gene:FBgn0010488 transcript:FBtr0334772) HSP 1 Score: 122.865 bits (307), Expect = 1.258e-27 Identity = 93/294 (31.63%), Postives = 147/294 (50.00%), Query Frame = 2
Query: 1490 YNHKQTERKQINLEISDNQVFIRSKSGYLPPQLRDKQKLLDSL--EELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKL---AITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDA--------MLALCKLLETCGKQIDVKH-KESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHV 2317
++ +Q ER Q + + R ++PP LR + L S + + R ++ LNK+TP+ F E E+ L++ + + +I IF KA+ E YS YAQ C +L A ++ P+ F L+ C+D F L+ +E D++ E+D EE+ AK + GN++FIGEL ++L+ V+ CI+EL A M L +LL+TCGK +D + KE MN+Y + R S+S + RIRF LKD+I++R+ NW R +
Sbjct: 474 HSQQQQERSQAGKASTPPPITGR----WIPPSLRPQHGLTQSEKNDAVFRRVRGILNKLTPEKFQELSDELLKLDLNSIAILKGVILLIFDKALDEPKYSSMYAQLCKRLSEEAPSFDKEPSNSSTFLRLLIAVCRDKFNNRLK--------------RDENDNRPPPENEADEEERRHLAKQRMLGNVKFIGELNKLDMLSKNVLHQCIMELFDKKKKRTAGTQEMCEDMECLAQLLKTCGKNLDSEQGKELMNQYFEKLERRSKSSEYPPRIRFMLKDVIELRQNNWVPRKL 749
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Fly
Match: eIF4G1 (gene:FBgn0023213 transcript:FBtr0112904) HSP 1 Score: 103.219 bits (256), Expect = 1.607e-21 Identity = 90/274 (32.85%), Postives = 145/274 (52.92%), Query Frame = 2
Query: 1619 EELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLAI------------TYTNNPAFKGQLLKKCQDMFETPLQDQLENVRNEWK-----EKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQID---VKHKE-----SMNKYISDISRLSQSVD-----LEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTL 2350
+EL R ++ LNK+TP+ F V+EI L I+ ++V +I +F KA+ E +SV+YA+ C +LA T +N+ F+ LL K + F Q+ ++ E K +K+ TD+ KA E+ LEE+ +K + + G +RFIGELF ++L GK+I +CI LL + + D + LCKLL T G + + V K+ S+ K I+ + ++ D + R+RF L+D+ID+R+ W Q + PKT+
Sbjct: 1000 DELVRRVRGILNKLTPERFDTLVEEIIKLKIDTPDKVDEVIVLVFEKAIDEPNFSVSYARLCQRLAAEVKVIDERMESETKSNSAHFRNALLDKTEQEF---TQNVSQSTAKEKKLQPIVDKIKKCTDANEKAELEAFLEEEERKIRRRSGGTVRFIGELFKISMLTGKIIYSCIDTLL-NPHSEDMLECLCKLLTTVGAKFEKTPVNSKDPSRCYSLEKSITRMQAIASKTDKDGARVSSRVRFMLQDVIDLRKNKW-----QTSRNEAPKTM 1264
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Fly
Match: eIF4G1 (gene:FBgn0023213 transcript:FBtr0289951) HSP 1 Score: 102.834 bits (255), Expect = 1.846e-21 Identity = 90/274 (32.85%), Postives = 145/274 (52.92%), Query Frame = 2
Query: 1619 EELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLAI------------TYTNNPAFKGQLLKKCQDMFETPLQDQLENVRNEWK-----EKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQID---VKHKE-----SMNKYISDISRLSQSVD-----LEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTL 2350
+EL R ++ LNK+TP+ F V+EI L I+ ++V +I +F KA+ E +SV+YA+ C +LA T +N+ F+ LL K + F Q+ ++ E K +K+ TD+ KA E+ LEE+ +K + + G +RFIGELF ++L GK+I +CI LL + + D + LCKLL T G + + V K+ S+ K I+ + ++ D + R+RF L+D+ID+R+ W Q + PKT+
Sbjct: 747 DELVRRVRGILNKLTPERFDTLVEEIIKLKIDTPDKVDEVIVLVFEKAIDEPNFSVSYARLCQRLAAEVKVIDERMESETKSNSAHFRNALLDKTEQEF---TQNVSQSTAKEKKLQPIVDKIKKCTDANEKAELEAFLEEEERKIRRRSGGTVRFIGELFKISMLTGKIIYSCIDTLL-NPHSEDMLECLCKLLTTVGAKFEKTPVNSKDPSRCYSLEKSITRMQAIASKTDKDGARVSSRVRFMLQDVIDLRKNKW-----QTSRNEAPKTM 1011
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Fly
Match: eIF4G1 (gene:FBgn0023213 transcript:FBtr0089243) HSP 1 Score: 102.834 bits (255), Expect = 1.846e-21 Identity = 90/274 (32.85%), Postives = 145/274 (52.92%), Query Frame = 2
Query: 1619 EELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLAI------------TYTNNPAFKGQLLKKCQDMFETPLQDQLENVRNEWK-----EKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQID---VKHKE-----SMNKYISDISRLSQSVD-----LEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTL 2350
+EL R ++ LNK+TP+ F V+EI L I+ ++V +I +F KA+ E +SV+YA+ C +LA T +N+ F+ LL K + F Q+ ++ E K +K+ TD+ KA E+ LEE+ +K + + G +RFIGELF ++L GK+I +CI LL + + D + LCKLL T G + + V K+ S+ K I+ + ++ D + R+RF L+D+ID+R+ W Q + PKT+
Sbjct: 747 DELVRRVRGILNKLTPERFDTLVEEIIKLKIDTPDKVDEVIVLVFEKAIDEPNFSVSYARLCQRLAAEVKVIDERMESETKSNSAHFRNALLDKTEQEF---TQNVSQSTAKEKKLQPIVDKIKKCTDANEKAELEAFLEEEERKIRRRSGGTVRFIGELFKISMLTGKIIYSCIDTLL-NPHSEDMLECLCKLLTTVGAKFEKTPVNSKDPSRCYSLEKSITRMQAIASKTDKDGARVSSRVRFMLQDVIDLRKNKW-----QTSRNEAPKTM 1011
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Fly
Match: NAT1 (gene:FBgn0010488 transcript:FBtr0087786) HSP 1 Score: 93.2041 bits (230), Expect = 1.030e-18 Identity = 64/192 (33.33%), Postives = 97/192 (50.52%), Query Frame = 2
Query: 1790 YAQFCYKL---AITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDA--------MLALCKLLETCGKQIDVKH-KESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHV 2317
YAQ C +L A ++ P+ F L+ C+D F L+ +E D++ E+D EE+ AK + GN++FIGEL ++L+ V+ CI+EL A M L +LL+TCGK +D + KE MN+Y + R S+S + RIRF LKD+I++R+ NW R +
Sbjct: 2 YAQLCKRLSEEAPSFDKEPSNSSTFLRLLIAVCRDKFNNRLK--------------RDENDNRPPPENEADEEERRHLAKQRMLGNVKFIGELNKLDMLSKNVLHQCIMELFDKKKKRTAGTQEMCEDMECLAQLLKTCGKNLDSEQGKELMNQYFEKLERRSKSSEYPPRIRFMLKDVIELRQNNWVPRKL 179
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Zebrafish
Match: eif4g3a (eukaryotic translation initiation factor 4 gamma, 3a [Source:ZFIN;Acc:ZDB-GENE-120810-2]) HSP 1 Score: 152.91 bits (385), Expect = 9.721e-37 Identity = 138/480 (28.75%), Postives = 237/480 (49.38%), Query Frame = 2
Query: 1268 YDRHYLIKMKDNLKSRQKPINMPATTEFSED--------IISNQPRVISCQLPPESSSSQTNNERANNKKQLPNRQYSKYKNYNHKQTERKQINLEISDNQVFIRSKSGYLPPQLR-----DKQKLLDSLEELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA---ITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKM-KALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKFN--LMTKKKNVSNSQNIARRNIQKNVSIKNLSCNSH-------------KVDEKTVLGPVLG-NWATGSGSKSKSHEAE 2593
YDR +L+ + QKP +P ++ D + S PR+IS P + + + A+ +Q+ + + N + +NL +SD+ ++++ + P R D + + +EL R ++S LNK+TPQ F + +K++ L I +E + +I+ +F KA+ E ++SV YA C LA + + P F+ LL +CQ FE +D++++V E K+K ++ S K + +LEE KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K + L++K Q R ++ + + NS ++DEK LGP NW GS +K+ ++E
Sbjct: 355 YDRDFLLGFQFMPACVQKPEGLPPISDVVLDKMNQNKLPLRSVDPRLISRGTPQDFTPA-----FADFSRQMAGGRGAPLLNVRRLPPRKIIVNLSVSDDVQLKKAENAWKPGMKREGVAEDPETI--RTQELFRKVRSILNKLTPQMFHQLMKQVTDLTINTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLATLKVPTADKPNTTVNFRKLLLNRCQKEFE---RDKVDDVVLERKQKEIDSATSPTEKERLQEELEEAKDKARRRSTGNIKFIGELFKLKMLTEPIMHDCVVKLLKN-HDDESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLHNWVSRRADQ----GPKTIEQIHKEAKLEEQEEQRKVHQQLLSKDMKRRPDQREQRSMREETWNTVPTTKNSRTIDPTKIPKISKPQIDEKIQLGPRAQINWVKGSSGGAKASDSE 819 HSP 2 Score: 87.8113 bits (216), Expect = 8.798e-17 Identity = 71/285 (24.91%), Postives = 136/285 (47.72%), Query Frame = 2
Query: 2918 VDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNI-SFFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVP--NHWETFLKVNNLEFTV-DLPGLSLCGGDPLNFIAEE----LEDMLSNNL-SNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSVQ---EYLKSKKEIIKRISNHKLENEIEILKSI--LNVASELNRDTLTRIYDLLITEKIVSHQAL 3727
++ +E+ R +G +L+ I+ Q+ +G + DD+ DIP IW + A+ L + + N+ F + K +LP A+ S LH ++ H+ VG ++ +S L W +P H F+ + LEFTV D+ +S P + EE LE +L ++ S+E+IF+++E ++D+ S + + ++I KA K +S + ++ + I+ + + E +++ L ++ L VA + + L +D L E ++S A
Sbjct: 994 LESTLERSQKAREHMGLLYYQLIQKGILPHSQLYKGFSEMLEQADDMAIDIPFIWLYLAELLSPLLKEGGINMRELFSELSKPLLPVGRAAILLSEILHLLCKQMSHRKVGDLWRESGLNWADFLPEGEHLPAFISQHKLEFTVSDISPVSPASAKP-SLSPEELFKQLEQLLLEDMSSDEQIFDWIEANLDESQMSSSPFLRALMTAICKAAVKDESTSCRVDTAIIQKRLPILHKYFDSDTERQLQALYALQSLIVALDQPPNLLRMFFDCLYDEDVISEDAF 1277
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Zebrafish
Match: eif4g3a (eukaryotic translation initiation factor 4 gamma, 3a [Source:ZFIN;Acc:ZDB-GENE-120810-2]) HSP 1 Score: 152.91 bits (385), Expect = 1.089e-36 Identity = 138/480 (28.75%), Postives = 237/480 (49.38%), Query Frame = 2
Query: 1268 YDRHYLIKMKDNLKSRQKPINMPATTEFSED--------IISNQPRVISCQLPPESSSSQTNNERANNKKQLPNRQYSKYKNYNHKQTERKQINLEISDNQVFIRSKSGYLPPQLR-----DKQKLLDSLEELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA---ITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKM-KALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKFN--LMTKKKNVSNSQNIARRNIQKNVSIKNLSCNSH-------------KVDEKTVLGPVLG-NWATGSGSKSKSHEAE 2593
YDR +L+ + QKP +P ++ D + S PR+IS P + + + A+ +Q+ + + N + +NL +SD+ ++++ + P R D + + +EL R ++S LNK+TPQ F + +K++ L I +E + +I+ +F KA+ E ++SV YA C LA + + P F+ LL +CQ FE +D++++V E K+K ++ S K + +LEE KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K + L++K Q R ++ + + NS ++DEK LGP NW GS +K+ ++E
Sbjct: 358 YDRDFLLGFQFMPACVQKPEGLPPISDVVLDKMNQNKLPLRSVDPRLISRGTPQDFTPA-----FADFSRQMAGGRGAPLLNVRRLPPRKIIVNLSVSDDVQLKKAENAWKPGMKREGVAEDPETI--RTQELFRKVRSILNKLTPQMFHQLMKQVTDLTINTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLATLKVPTADKPNTTVNFRKLLLNRCQKEFE---RDKVDDVVLERKQKEIDSATSPTEKERLQEELEEAKDKARRRSTGNIKFIGELFKLKMLTEPIMHDCVVKLLKN-HDDESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLHNWVSRRADQ----GPKTIEQIHKEAKLEEQEEQRKVHQQLLSKDMKRRPDQREQRSMREETWNTVPTTKNSRTIDPTKIPKISKPQIDEKIQLGPRAQINWVKGSSGGAKASDSE 822 HSP 2 Score: 87.4261 bits (215), Expect = 1.070e-16 Identity = 71/285 (24.91%), Postives = 136/285 (47.72%), Query Frame = 2
Query: 2918 VDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNI-SFFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVP--NHWETFLKVNNLEFTV-DLPGLSLCGGDPLNFIAEE----LEDMLSNNL-SNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSVQ---EYLKSKKEIIKRISNHKLENEIEILKSI--LNVASELNRDTLTRIYDLLITEKIVSHQAL 3727
++ +E+ R +G +L+ I+ Q+ +G + DD+ DIP IW + A+ L + + N+ F + K +LP A+ S LH ++ H+ VG ++ +S L W +P H F+ + LEFTV D+ +S P + EE LE +L ++ S+E+IF+++E ++D+ S + + ++I KA K +S + ++ + I+ + + E +++ L ++ L VA + + L +D L E ++S A
Sbjct: 997 LESTLERSQKAREHMGLLYYQLIQKGILPHSQLYKGFSEMLEQADDMAIDIPFIWLYLAELLSPLLKEGGINMRELFSELSKPLLPVGRAAILLSEILHLLCKQMSHRKVGDLWRESGLNWADFLPEGEHLPAFISQHKLEFTVSDISPVSPASAKP-SLSPEELFKQLEQLLLEDMSSDEQIFDWIEANLDESQMSSSPFLRALMTAICKAAVKDESTSCRVDTAIIQKRLPILHKYFDSDTERQLQALYALQSLIVALDQPPNLLRMFFDCLYDEDVISEDAF 1280
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Zebrafish
Match: eif4g3a (eukaryotic translation initiation factor 4 gamma, 3a [Source:ZFIN;Acc:ZDB-GENE-120810-2]) HSP 1 Score: 150.984 bits (380), Expect = 5.400e-36 Identity = 138/480 (28.75%), Postives = 237/480 (49.38%), Query Frame = 2
Query: 1268 YDRHYLIKMKDNLKSRQKPINMPATTEFSED--------IISNQPRVISCQLPPESSSSQTNNERANNKKQLPNRQYSKYKNYNHKQTERKQINLEISDNQVFIRSKSGYLPPQLR-----DKQKLLDSLEELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA---ITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKM-KALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKFN--LMTKKKNVSNSQNIARRNIQKNVSIKNLSCNSH-------------KVDEKTVLGPVLG-NWATGSGSKSKSHEAE 2593
YDR +L+ + QKP +P ++ D + S PR+IS P + + + A+ +Q+ + + N + +NL +SD+ ++++ + P R D + + +EL R ++S LNK+TPQ F + +K++ L I +E + +I+ +F KA+ E ++SV YA C LA + + P F+ LL +CQ FE +D++++V E K+K ++ S K + +LEE KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K + L++K Q R ++ + + NS ++DEK LGP NW GS +K+ ++E
Sbjct: 750 YDRDFLLGFQFMPACVQKPEGLPPISDVVLDKMNQNKLPLRSVDPRLISRGTPQDFTPA-----FADFSRQMAGGRGAPLLNVRRLPPRKIIVNLSVSDDVQLKKAENAWKPGMKREGVAEDPETI--RTQELFRKVRSILNKLTPQMFHQLMKQVTDLTINTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLATLKVPTADKPNTTVNFRKLLLNRCQKEFE---RDKVDDVVLERKQKEIDSATSPTEKERLQEELEEAKDKARRRSTGNIKFIGELFKLKMLTEPIMHDCVVKLLKN-HDDESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLHNWVSRRADQ----GPKTIEQIHKEAKLEEQEEQRKVHQQLLSKDMKRRPDQREQRSMREETWNTVPTTKNSRTIDPTKIPKISKPQIDEKIQLGPRAQINWVKGSSGGAKASDSE 1214 HSP 2 Score: 88.5817 bits (218), Expect = 5.144e-17 Identity = 71/289 (24.57%), Postives = 137/289 (47.40%), Query Frame = 2
Query: 2906 IEKWVDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNI-SFFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVP--NHWETFLKVNNLEFTV-DLPGLSLCGGDPLNFIAEE----LEDMLSNNL-SNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSVQ---EYLKSKKEIIKRISNHKLENEIEILKSI--LNVASELNRDTLTRIYDLLITEKIVSHQAL 3727
+ ++ +E+ R +G +L+ I+ Q+ +G + DD+ DIP IW + A+ L + + N+ F + K +LP A+ S LH ++ H+ VG ++ +S L W +P H F+ + LEFTV D+ +S P + EE LE +L ++ S+E+IF+++E ++D+ S + + ++I KA K +S + ++ + I+ + + E +++ L ++ L VA + + L +D L E ++S A
Sbjct: 1385 VRMGLESTLERSQKAREHMGLLYYQLIQKGILPHSQLYKGFSEMLEQADDMAIDIPFIWLYLAELLSPLLKEGGINMRELFSELSKPLLPVGRAAILLSEILHLLCKQMSHRKVGDLWRESGLNWADFLPEGEHLPAFISQHKLEFTVSDISPVSPASAKP-SLSPEELFKQLEQLLLEDMSSDEQIFDWIEANLDESQMSSSPFLRALMTAICKAAVKDESTSCRVDTAIIQKRLPILHKYFDSDTERQLQALYALQSLIVALDQPPNLLRMFFDCLYDEDVISEDAF 1672
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Zebrafish
Match: eif4g3b (eukaryotic translation initiation factor 4 gamma, 3b [Source:ZFIN;Acc:ZDB-GENE-090312-49]) HSP 1 Score: 133.265 bits (334), Expect = 1.367e-30 Identity = 108/351 (30.77%), Postives = 180/351 (51.28%), Query Frame = 2
Query: 1619 EELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA---ITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVK-KAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKFNLMTKKKNVSNSQNIARRNIQKNVSIKNLS----------CNSHKVDEKTVLGP-VLGNWATGS--GSKSKSHEAEGWTP 2605
+EL R ++S LNK+TPQ F + +K++ L I+ +E + +I+ +F KA+ E ++SV Y C LA + T+ P F+ LL +CQ FE +D++++ E K + L + + + E+ K KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ +A E K + K+ + R V + S + +DEK LGP NW GS G+ +K+ E++ P
Sbjct: 866 QELFRKVRSILNKLTPQMFSQLMKQVTDLTIDTEERLKGVIDLVFEKAINEPSFSVAYGNMCSCLATLKVPMTDKPNSTVNFRKLLLNRCQKEFE---KDKMDDDAFEKKHRELEAATASSERERLQEELEEAKDKARRRSIGNIKFIGELFKLRMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLHNWVSRRADQ----GPKTIEQIHKDAKLEEQEEQRKVHQQLLSKDNKRRPVVQREETWSTVPMTKNSRTIDPAKIPKFSKPAIDEKIQLGPRAQVNWMKGSSGGAGAKASESDASRP 1208 HSP 2 Score: 78.1814 bits (191), Expect = 7.747e-14 Identity = 64/290 (22.07%), Postives = 133/290 (45.86%), Query Frame = 2
Query: 2918 VDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNI-SFFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNHWE--TFLKVNNLEFTVDLPGLSLCGGDPLNFIA------EELED-----MLSNNLSNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSVQ---EYLKSKKEIIKRISNHKLENEIEILKSILNVASELNRDT--LTRIYDLLITEKIVSHQAL 3727
V+ +E+ R +G L +LL ++ Q +G + DD+ DIP IW + A+ + + + ++ F K +LP + FS LH ++ HK VG ++ S L W +P + +F+ + LEFT+ G +P ++ EEL +L + + +E+IF+++E ++D+ + + + +++ KA K SS + ++++ ++ + N E +++ L ++ + +L++ L +D L E ++S A
Sbjct: 1377 VESTLERSQITRDHMGQLLFQLLQAGVLPKLQFFKGFSETLELADDMAIDIPHIWLYLAELVTPVLREGGISMRELFSEFSKPLLPVGRAGILFSEILHLLCKQMSHKKVGSLWRDSGLSWADFIPETEDVHSFITEHKLEFTLS------DGSNPTEAVSKRTLSPEELNKQLEKLLLEDMVGDEQIFDWVEANLDESEMSSAPFVRALMTAVCKAAVKTEGSSCKVDLSIIQTRLPVLHKYLNSDTERQLQALYALQALIVKLDQPANLLRMFFDCLYDEDVISEDAF 1660
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Zebrafish
Match: eif4g3b (eukaryotic translation initiation factor 4 gamma, 3b [Source:ZFIN;Acc:ZDB-GENE-090312-49]) HSP 1 Score: 132.88 bits (333), Expect = 1.567e-30 Identity = 108/351 (30.77%), Postives = 180/351 (51.28%), Query Frame = 2
Query: 1619 EELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA---ITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVK-KAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKFNLMTKKKNVSNSQNIARRNIQKNVSIKNLS----------CNSHKVDEKTVLGP-VLGNWATGS--GSKSKSHEAEGWTP 2605
+EL R ++S LNK+TPQ F + +K++ L I+ +E + +I+ +F KA+ E ++SV Y C LA + T+ P F+ LL +CQ FE +D++++ E K + L + + + E+ K KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ +A E K + K+ + R V + S + +DEK LGP NW GS G+ +K+ E++ P
Sbjct: 867 QELFRKVRSILNKLTPQMFSQLMKQVTDLTIDTEERLKGVIDLVFEKAINEPSFSVAYGNMCSCLATLKVPMTDKPNSTVNFRKLLLNRCQKEFE---KDKMDDDAFEKKHRELEAATASSERERLQEELEEAKDKARRRSIGNIKFIGELFKLRMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLHNWVSRRADQ----GPKTIEQIHKDAKLEEQEEQRKVHQQLLSKDNKRRPVVQREETWSTVPMTKNSRTIDPAKIPKFSKPAIDEKIQLGPRAQVNWMKGSSGGAGAKASESDASRP 1209 HSP 2 Score: 78.1814 bits (191), Expect = 7.340e-14 Identity = 64/290 (22.07%), Postives = 133/290 (45.86%), Query Frame = 2
Query: 2918 VDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNI-SFFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNHWE--TFLKVNNLEFTVDLPGLSLCGGDPLNFIA------EELED-----MLSNNLSNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSVQ---EYLKSKKEIIKRISNHKLENEIEILKSILNVASELNRDT--LTRIYDLLITEKIVSHQAL 3727
V+ +E+ R +G L +LL ++ Q +G + DD+ DIP IW + A+ + + + ++ F K +LP + FS LH ++ HK VG ++ S L W +P + +F+ + LEFT+ G +P ++ EEL +L + + +E+IF+++E ++D+ + + + +++ KA K SS + ++++ ++ + N E +++ L ++ + +L++ L +D L E ++S A
Sbjct: 1378 VESTLERSQITRDHMGQLLFQLLQAGVLPKLQFFKGFSETLELADDMAIDIPHIWLYLAELVTPVLREGGISMRELFSEFSKPLLPVGRAGILFSEILHLLCKQMSHKKVGSLWRDSGLSWADFIPETEDVHSFITEHKLEFTLS------DGSNPTEAVSKRTLSPEELNKQLEKLLLEDMVGDEQIFDWVEANLDESEMSSAPFVRALMTAVCKAAVKTEGSSCKVDLSIIQTRLPVLHKYLNSDTERQLQALYALQALIVKLDQPANLLRMFFDCLYDEDVISEDAF 1661
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Xenopus
Match: eif4g3 (eukaryotic translation initiation factor 4 gamma, 3 [Source:NCBI gene;Acc:100216088]) HSP 1 Score: 175.637 bits (444), Expect = 1.786e-43 Identity = 235/943 (24.92%), Postives = 432/943 (45.81%), Query Frame = 2
Query: 1268 YDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQPRVI-------SCQLPPESSSSQTNNER-ANNKKQLPNRQYSKYKNYNHKQTERKQI--NLEISDNQVFIRSKSGYLPPQLRDK-QKLLDSL--EELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA---ITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLN-ETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKF-NLMTKKKNVSNSQNI----------ARRN-------IQKNVSIKNLS-----CNSHKV-------DEKTV---LGPVLGNWATGSGSKSKSHEAEGWTPVSQKPKGGAMRYTSNSRSREQSPQVILANKYRNLESSAEASG-ADSRSSTPPMIRPITKAFRIIEQE---------VTDEEM---AEIIFNDLLSTGNFD-----IKKWLTLRKVQPIIEKWVDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFENI---FKSILPNNDVALCFSTALHEASSRL--GHKIVGQVFHKSLKWEKIVPNH--WETFLKVNNLEFTV---DLPGLSLCGGDPLNFIAEELEDMLSN-----NLSNEKIFEFLETHVDK-----PTWKKSECTKTITSSILKALRKIPESSVQ---EYLKSKKEIIKRISNHKLENEIEILKSILNVASELNR--DTLTRIYDLLITEKIVSHQAL------KDYCDNE----SLKSENNF 3772
YDR +L+ + QKP +P ++ D I NQP++ Q P+ + + + R A + +P ++ ++ E ++I N+ +SD+ +S++ + P RD + DS+ + L R ++S LNK+TPQ F + +K++ L ++ +E + +I+ +F KA+ E ++SV YA C LA + + P F+ LL +CQ FE +D+ ++ E K+K L T + K+ + +LEE KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K LMTK+K Q + A+ N K ++ L ++H + +K+V L VLG + GS + K+ + + + P+ G + S+ S + ++ + L++ + + A+ +T P RP A ++ + +++EEM ++ I ++ L ++ +++ + + + V+ +E+ R +G L +L+ +S G + DD+ DIP IW + A+ + + K IS E I K++LP S LH ++ G K + F + E I+P ++F+ NLEFT+ P +L + AEEL L ++E+IF+++E +D+ PT+ ++ T ++IL SS + +LK + ++ + + E E++ L ++ + +L++ + L +D L E+++S A KD + E +LKS F
Sbjct: 558 YDRDFLLGFQYMPACVQKPEGLPPISDVVLDKI-NQPKLPIRALDARMLQRGPDFTPAYADFGRPAPGGRGMPLLSVGPRRSQPGQRREPRKIIMNISVSDDVHLKKSENAWKPSVKRDSLPEDPDSIRTQALFRKVRSILNKLTPQMFNQLMKQVTDLTVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLATLKVPMADKPGSTVNFRKLLLNRCQKEFE---KDKADDDVFEKKQKDLELATTPEEKSRLQDELEESKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLSNWVSRRAYQ----GPKTIEQIHKEAKIEEQEEQRKLQQLMTKEKRRPGVQRVDEGGWNTVQGAKNNRVLDPSKFLKITKVRRLPPPVALSSAHALLLWLGGSSDKSVTCDLTVVLGFSSRGSTGREKNDKPSAPSSIPSAPRPGTLLRGSSKDSADNQEEL----RRETLQTVKQLTNVAEPDKNTVPERRPKEPARTLVSKSEPSEPDKPTLSEEEMERKSKAIIDEFLHINDYKEAMQCVEELGANGPLSVFVRQGVESTLERSQITRDHMGQLLYQLVQSGKLSKLDFFTGFSETLELADDMAIDIPHIWLYLAELVTPML--KEGGISLRELITEFGKALLPVGRGGELLSEILHLLCKQMVRGKKPPRRFF---VHPEGILPEGEDVQSFVSEKNLEFTLSDSSSPSENLSKKE---MSAEELHKRLEQLIIQEKANDEQIFDWVEASLDESQMSSPTFLRALMTAVCKAAILGDC-----SSCRVDITFLKQRVPVLLKYLDSDTERELQALYALQALIVKLDQPPNVLRMFFDCLYDEEVISEDAFYKWESSKDPAEQEGKGVALKSVTAF 1474
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Xenopus
Match: eif4g3 (eukaryotic translation initiation factor 4 gamma, 3 [Source:NCBI gene;Acc:100216088]) HSP 1 Score: 160.614 bits (405), Expect = 7.032e-39 Identity = 160/554 (28.88%), Postives = 265/554 (47.83%), Query Frame = 2
Query: 1049 PLSVKSFERMEFEEFDLQNKAP-ESSIRLDIEEDVATCSSEDEVHIDLKLSSSNYHLPVNKKESDETILKRKLIYDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQPRVI-------SCQLPPESSSSQTNNER-ANNKKQLPNRQYSKYKNYNHKQTERKQI--NLEISDNQVFIRSKSGYLPPQLRDK-QKLLDSL--EELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA---ITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLN-ETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKF-NLMTKKKNVSNSQNIARRNIQKNVSIKN---------LSCNSHKVDEKTVLGPVL--GNWATGSGSKSKSHEAEGWTP 2605
P++ ME E+ L N P E + ++ + A SE E + P +K KR+ YDR +L+ + QKP +P ++ D I NQP++ Q P+ + + + R A + +P ++ ++ E ++I N+ +SD+ +S++ + P RD + DS+ + L R ++S LNK+TPQ F + +K++ L ++ +E + +I+ +F KA+ E ++SV YA C LA + + P F+ LL +CQ FE +D+ ++ E K+K L T + K+ + +LEE KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K LMTK+K Q + KN L +DEK L P G+W GS +K+ E E P
Sbjct: 569 PVTYSGTSTMEVEQLYLNNAKPQEQNGEVEPIRNGADAVSESEGTDIPSGTEEGLAYPYSKSADG----KRQ--YDRDFLLGFQYMPACVQKPEGLPPISDVVLDKI-NQPKLPIRALDARMLQRGPDFTPAYADFGRPAPGGRGMPLLSVGPRRSQPGQRREPRKIIMNISVSDDVHLKKSENAWKPSVKRDSLPEDPDSIRTQALFRKVRSILNKLTPQMFNQLMKQVTDLTVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLATLKVPMADKPGSTVNFRKLLLNRCQKEFE---KDKADDDVFEKKQKDLELATTPEEKSRLQDELEESKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLSNWVSRRAYQ----GPKTIEQIHKEAKIEEQEEQRKLQQLMTKEKRRPGVQRVDEGGWNTVQGAKNNRVLDPSKFLKITKPTIDEKIQLVPKAQPGSWLKGSSGGAKASETESLRP 1107 HSP 2 Score: 53.9138 bits (128), Expect = 2.441e-6 Identity = 77/325 (23.69%), Postives = 140/325 (43.08%), Query Frame = 2
Query: 2903 IIEKWVDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFENI---FKSILPNNDVALCFSTALHEASSRL--GHKIVGQVFHKSLKWEKIVPNH--WETFLKVNNLEFTV---DLPGLSLCGGDPLNFIAEELEDMLSN-----NLSNEKIFEFLETHVDK-----PTWKKSECTKTITSSILKALRKIPESSVQ---EYLKSKKEIIKRISNHKLENEIEILKSILNVASELNR--DTLTRIYDLLITEKIVSHQAL------KDYCDNE----SLKSENNF 3772
+ + V+ +E+ R +G L +L+ +S G + DD+ DIP IW + A+ + + K IS E I K++LP S LH ++ G K + F + E I+P ++F+ NLEFT+ P +L + AEEL L ++E+IF+++E +D+ PT+ ++ T ++IL SS + +LK + ++ + + E E++ L ++ + +L++ + L +D L E+++S A KD + E +LKS F
Sbjct: 1259 FVRQGVESTLERSQITRDHMGQLLYQLVQSGKLSKLDFFTGFSETLELADDMAIDIPHIWLYLAELVTPML--KEGGISLRELITEFGKALLPVGRGGELLSEILHLLCKQMVRGKKPPRRFF---VHPEGILPEGEDVQSFVSEKNLEFTLSDSSSPSENLSKKE---MSAEELHKRLEQLIIQEKANDEQIFDWVEASLDESQMSSPTFLRALMTAVCKAAILGDC-----SSCRVDITFLKQRVPVLLKYLDSDTERELQALYALQALIVKLDQPPNVLRMFFDCLYDEEVISEDAFYKWESSKDPAEQEGKGVALKSVTAF 1570
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Xenopus
Match: eif4g3 (eukaryotic translation initiation factor 4 gamma, 3 [Source:NCBI gene;Acc:100216088]) HSP 1 Score: 159.073 bits (401), Expect = 2.305e-38 Identity = 144/480 (30.00%), Postives = 239/480 (49.79%), Query Frame = 2
Query: 1268 YDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQPRVI-------SCQLPPESSSSQTNNER-ANNKKQLPNRQYSKYKNYNHKQTERKQI--NLEISDNQVFIRSKSGYLPPQLRDK-QKLLDSL--EELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA---ITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLN-ETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKF-NLMTKKKNVSNSQNIARRNIQKNVSIKN---------LSCNSHKVDEKTVLGPVL--GNWATGSGSKSKSHEAEGWTP 2605
YDR +L+ + QKP +P ++ D I NQP++ Q P+ + + + R A + +P ++ ++ E ++I N+ +SD+ +S++ + P RD + DS+ + L R ++S LNK+TPQ F + +K++ L ++ +E + +I+ +F KA+ E ++SV YA C LA + + P F+ LL +CQ FE +D+ ++ E K+K L T + K+ + +LEE KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K LMTK+K Q + KN L +DEK L P G+W GS +K+ E E P
Sbjct: 606 YDRDFLLGFQYMPACVQKPEGLPPISDVVLDKI-NQPKLPIRALDARMLQRGPDFTPAYADFGRPAPGGRGMPLLSVGPRRSQPGQRREPRKIIMNISVSDDVHLKKSENAWKPSVKRDSLPEDPDSIRTQALFRKVRSILNKLTPQMFNQLMKQVTDLTVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLATLKVPMADKPGSTVNFRKLLLNRCQKEFE---KDKADDDVFEKKQKDLELATTPEEKSRLQDELEESKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLSNWVSRRAYQ----GPKTIEQIHKEAKIEEQEEQRKLQQLMTKEKRRPGVQRVDEGGWNTVQGAKNNRVLDPSKFLKITKPTIDEKIQLVPKAQPGSWLKGSSGGAKASETESLRP 1076 HSP 2 Score: 53.9138 bits (128), Expect = 2.812e-6 Identity = 77/325 (23.69%), Postives = 140/325 (43.08%), Query Frame = 2
Query: 2903 IIEKWVDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFENI---FKSILPNNDVALCFSTALHEASSRL--GHKIVGQVFHKSLKWEKIVPNH--WETFLKVNNLEFTV---DLPGLSLCGGDPLNFIAEELEDMLSN-----NLSNEKIFEFLETHVDK-----PTWKKSECTKTITSSILKALRKIPESSVQ---EYLKSKKEIIKRISNHKLENEIEILKSILNVASELNR--DTLTRIYDLLITEKIVSHQAL------KDYCDNE----SLKSENNF 3772
+ + V+ +E+ R +G L +L+ +S G + DD+ DIP IW + A+ + + K IS E I K++LP S LH ++ G K + F + E I+P ++F+ NLEFT+ P +L + AEEL L ++E+IF+++E +D+ PT+ ++ T ++IL SS + +LK + ++ + + E E++ L ++ + +L++ + L +D L E+++S A KD + E +LKS F
Sbjct: 1239 FVRQGVESTLERSQITRDHMGQLLYQLVQSGKLSKLDFFTGFSETLELADDMAIDIPHIWLYLAELVTPML--KEGGISLRELITEFGKALLPVGRGGELLSEILHLLCKQMVRGKKPPRRFF---VHPEGILPEGEDVQSFVSEKNLEFTLSDSSSPSENLSKKE---MSAEELHKRLEQLIIQEKANDEQIFDWVEASLDESQMSSPTFLRALMTAVCKAAILGDC-----SSCRVDITFLKQRVPVLLKYLDSDTERELQALYALQALIVKLDQPPNVLRMFFDCLYDEEVISEDAFYKWESSKDPAEQEGKGVALKSVTAF 1550
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Xenopus
Match: eif4g3 (eukaryotic translation initiation factor 4 gamma, 3 [Source:NCBI gene;Acc:100216088]) HSP 1 Score: 158.688 bits (400), Expect = 2.407e-38 Identity = 144/480 (30.00%), Postives = 239/480 (49.79%), Query Frame = 2
Query: 1268 YDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQPRVI-------SCQLPPESSSSQTNNER-ANNKKQLPNRQYSKYKNYNHKQTERKQI--NLEISDNQVFIRSKSGYLPPQLRDK-QKLLDSL--EELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA---ITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLN-ETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKF-NLMTKKKNVSNSQNIARRNIQKNVSIKN---------LSCNSHKVDEKTVLGPVL--GNWATGSGSKSKSHEAEGWTP 2605
YDR +L+ + QKP +P ++ D I NQP++ Q P+ + + + R A + +P ++ ++ E ++I N+ +SD+ +S++ + P RD + DS+ + L R ++S LNK+TPQ F + +K++ L ++ +E + +I+ +F KA+ E ++SV YA C LA + + P F+ LL +CQ FE +D+ ++ E K+K L T + K+ + +LEE KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K LMTK+K Q + KN L +DEK L P G+W GS +K+ E E P
Sbjct: 559 YDRDFLLGFQYMPACVQKPEGLPPISDVVLDKI-NQPKLPIRALDARMLQRGPDFTPAYADFGRPAPGGRGMPLLSVGPRRSQPGQRREPRKIIMNISVSDDVHLKKSENAWKPSVKRDSLPEDPDSIRTQALFRKVRSILNKLTPQMFNQLMKQVTDLTVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLATLKVPMADKPGSTVNFRKLLLNRCQKEFE---KDKADDDVFEKKQKDLELATTPEEKSRLQDELEESKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLSNWVSRRAYQ----GPKTIEQIHKEAKIEEQEEQRKLQQLMTKEKRRPGVQRVDEGGWNTVQGAKNNRVLDPSKFLKITKPTIDEKIQLVPKAQPGSWLKGSSGGAKASETESLRP 1029
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Xenopus
Match: eif4g3 (eukaryotic translation initiation factor 4 gamma, 3 [Source:NCBI gene;Acc:100216088]) HSP 1 Score: 157.918 bits (398), Expect = 5.491e-38 Identity = 170/604 (28.15%), Postives = 282/604 (46.69%), Query Frame = 2
Query: 986 DKEETEQSKKE---DDDILLESKT-----PLSVKSFERMEFEEFDLQNKAPE---------------------SSIRLDIEEDVATCSSEDEVHIDLKLSSSNYHLPVNKKESDETILKRKLIYDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQPRVI-------SCQLPPESSSSQTNNER-ANNKKQLPNRQYSKYKNYNHKQTERKQI--NLEISDNQVFIRSKSGYLPPQLRDK-QKLLDSL--EELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA---ITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLN-ETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKF-NLMTK-KKNVSNSQNIARRNIQKNVSIKN---------LSCNSHKVDEKTVLGPVL--GNWATGSGSKSKSHEAEGWTP 2605
D EE QS+ E +DD L + T P++ ME E+ L N P+ + I EE +A S+ + + + P + +D KR+ YDR +L+ + QKP +P ++ D I NQP++ Q P+ + + + R A + +P ++ ++ E ++I N+ +SD+ +S++ + P RD + DS+ + L R ++S LNK+TPQ F + +K++ L ++ +E + +I+ +F KA+ E ++SV YA C LA + + P F+ LL +CQ FE +D+ ++ E K+K L T + K+ + +LEE KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K LMTK K+ + Q + KN L +DEK L P G+W GS +K+ E E P
Sbjct: 522 DIEEISQSETEPQYEDDPLEDKVTELDQDPVTYSGTSTMEVEQLYLNNAKPQEQNGEVEPIRNGADAVSESEGTDIPSGTEEGLAYPYSKAGIGAAEQPPDPSQEPPAPPQSADG---KRQ--YDRDFLLGFQYMPACVQKPEGLPPISDVVLDKI-NQPKLPIRALDARMLQRGPDFTPAYADFGRPAPGGRGMPLLSVGPRRSQPGQRREPRKIIMNISVSDDVHLKKSENAWKPSVKRDSLPEDPDSIRTQALFRKVRSILNKLTPQMFNQLMKQVTDLTVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLATLKVPMADKPGSTVNFRKLLLNRCQKEFE---KDKADDDVFEKKQKDLELATTPEEKSRLQDELEESKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLSNWVSRRAYQ----GPKTIEQIHKEAKIEEQEEQRKLQQLMTKEKRRPAGVQRVDEGGWNTVQGAKNNRVLDPSKFLKITKPTIDEKIQLVPKAQPGSWLKGSSGGAKASETESLRP 1111 HSP 2 Score: 55.4546 bits (132), Expect = 1.008e-6 Identity = 77/326 (23.62%), Postives = 142/326 (43.56%), Query Frame = 2
Query: 2903 IIEKWVDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFENI---FKSILPNNDVALCFSTALHEASSRLGHK---IVGQVFHKSLKWEKIVPNH--WETFLKVNNLEFTV---DLPGLSLCGGDPLNFIAEELEDMLSN-----NLSNEKIFEFLETHVDK-----PTWKKSECTKTITSSILKALRKIPESSVQ---EYLKSKKEIIKRISNHKLENEIEILKSILNVASELNR--DTLTRIYDLLITEKIVSHQAL------KDYCDNE----SLKSENNF 3772
+ + V+ +E+ R +G L +L+ +S G + DD+ DIP IW + A+ + + K IS E I K++LP S LH ++ HK + ++F + E I+P ++F+ NLEFT+ P +L + AEEL L ++E+IF+++E +D+ PT+ ++ T ++IL SS + +LK + ++ + + E E++ L ++ + +L++ + L +D L E+++S A KD + E +LKS F
Sbjct: 1274 FVRQGVESTLERSQITRDHMGQLLYQLVQSGKLSKLDFFTGFSETLELADDMAIDIPHIWLYLAELVTPML--KEGGISLRELITEFGKALLPVGRGGELLSEILHLLCKQMSHKQETPLWKLFF--VHPEGILPEGEDVQSFVSEKNLEFTLSDSSSPSENLSKKE---MSAEELHKRLEQLIIQEKANDEQIFDWVEASLDESQMSSPTFLRALMTAVCKAAILGDC-----SSCRVDITFLKQRVPVLLKYLDSDTERELQALYALQALIVKLDQPPNVLRMFFDCLYDEEVISEDAFYKWESSKDPAEQEGKGVALKSVTAF 1587
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Mouse
Match: Eif4g3 (eukaryotic translation initiation factor 4 gamma, 3 [Source:MGI Symbol;Acc:MGI:1923935]) HSP 1 Score: 186.808 bits (473), Expect = 2.018e-47 Identity = 230/923 (24.92%), Postives = 402/923 (43.55%), Query Frame = 2
Query: 1268 YDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQPRVISCQLPPE--SSSSQTNNERANNKKQLPNRQYSKYKNYNHKQTE-------RKQINLEISDNQVFIRSKSGYLPPQLRDKQKLLDSLE-----ELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFC---YKLAITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDS-KMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKFN-LMTKKKNVSNSQNIARRNIQKNVSIKN---------LSCNSHKVDEKTVLGP-------VLGNWATGSGSKSKSHEAEGWTPV------------------SQKP------------KGGAMRYTSNSRSREQSPQVILANKYRNLESSAE-ASGADSRSSTPPMIR------PITKAFRIIEQEVTDEEMAEIIFNDLLSTGNFD-----IKKWLTLRKVQPIIEKWVDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFENIF---KSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNHWET--FLKVNNLEFTVDLPGLSLCGGDPLN---FIAEELED-----MLSNNLSNEKIFEFLETHVDK-----PTWKKSECTKTITSSILKALRKIPESSVQEYLKSKKEIIKRISNHKLENEIEILKSILNVASELNR--DTLTRIYDLLITEKIVSHQAL 3727
YDR +L+ ++ QKP +P ++ D I NQPR+ L P A+ +Q P + N ++++ RK I + + ++ ++++ + P Q RD D E EL R ++S LNK+TPQ F + +K++ L ++ +E + +I+ +F KA+ E ++SV YA C L + + P F+ LL +CQ FE +D+ ++ E K+K L + + + +LEE KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K LMTK+K Q + KN L +DEK L P G+ S+S + TP+ S KP +G + N EQ +++ K AE AS RS T + + P + E+EV E ++ I ++ L +F I++ + ++ V+ +E+ R +G L +L+ + +S Q +G + DD+ DIP IW + A+ + + K IS E I K +LP + S LH ++ HK VG ++ ++ L W+ +P + FL L+FT C + L+ AEEL ++ +E+IF+++E ++D+ PT+ ++ T ++I+ + +K + I+ + + E E++ L ++ +L++ + L +D L E+++S A
Sbjct: 61 YDREFLLDIQFMPACIQKPEGLPPISDVVLDKI-NQPRLSMRTLDPRILPRGPDFTPAFADFPRQTPGGRGVPLLNVGPRRSQPGQRREPRKIITVSVKEDVHLRKAENAWKPSQKRDSHA--DDPESIKTQELFRKVRSILNKLTPQMFNQLMKQVSALTVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLVTLKVPMADKPGNTVNFRKLLLNRCQKEFE---KDKADDDVFEKKQKELEAASAPEERTRLHDELEEAKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLCNWVSRRADQ----GPKTIEQIHKEAKIEEQEEQRKVQQLMTKEKRRPGVQRVDEGGWNTVQGAKNSRVLDPSKFLKITKPTIDEKIQLVPKAQLGSWGKGSSGGAKASESAPSGSPSATPLEFDSRRALTSRGSMGREKSDKPIPAGTARPNTFLRGSSKDLLDNQSQEEQRREMLETVKQLTGGLDAERASTEADRSKTRELAKSEMCAVPAPDKPALSEEEV--ERKSKSIIDEFLHINDFKEATQCIEELSAQGPLHVFVKVGVEFTLERSQITRDHMGHLLYQLVQSEKLSKQDFFKGFSETLELADDMAIDIPHIWLYLAELVTPML--KGGGISMRELIVEFSKPLLPVGRAGVLLSEILHLLCRQMSHKKVGALWREADLSWKDFLPEGEDVHHFLLEQKLDFT---ESEGPCSSEALSKKELSAEELSQRLEKLIMEEKADDERIFDWVEANLDESQMSSPTFLRALMTAVCKAAIIADCSTFRVDTA--VIKQRVPILLKYLDSDTEKELQALYALQASIVKLDQPANLLRMFFDCLYDEEVISEDAF 963
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Mouse
Match: Eif4g3 (eukaryotic translation initiation factor 4 gamma, 3 [Source:MGI Symbol;Acc:MGI:1923935]) HSP 1 Score: 147.517 bits (371), Expect = 4.879e-35 Identity = 126/418 (30.14%), Postives = 211/418 (50.48%), Query Frame = 2
Query: 1268 YDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQPRVISCQLPPE--SSSSQTNNERANNKKQLPNRQYSKYKNYNHKQTE-------RKQINLEISDNQVFIRSKSGYLPPQLRDKQKLLDSLE-----ELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFC---YKLAITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDS-KMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKF-NLMTKKKNVSNSQNI 2449
YDR +L+ ++ QKP +P ++ D I NQPR+ L P A+ +Q P + N ++++ RK I + + ++ ++++ + P Q RD D E EL R ++S LNK+TPQ F + +K++ L ++ +E + +I+ +F KA+ E ++SV YA C L + + P F+ LL +CQ FE +D+ ++ E K+K L + + + +LEE KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K LMTK+K Q +
Sbjct: 619 YDREFLLDIQFMPACIQKPEGLPPISDVVLDKI-NQPRLSMRTLDPRILPRGPDFTPAFADFPRQTPGGRGVPLLNVGPRRSQPGQRREPRKIITVSVKEDVHLRKAENAWKPSQKRDSHA--DDPESIKTQELFRKVRSILNKLTPQMFNQLMKQVSALTVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLVTLKVPMADKPGNTVNFRKLLLNRCQKEFE---KDKADDDVFEKKQKELEAASAPEERTRLHDELEEAKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLCNWVSRRADQ----GPKTIEQIHKEAKIEEQEEQRKVQQLMTKEKRRPGVQRV 1025 HSP 2 Score: 65.855 bits (159), Expect = 4.544e-10 Identity = 67/291 (23.02%), Postives = 129/291 (44.33%), Query Frame = 2
Query: 2918 VDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFENIF---KSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNHWET--FLKVNNLEFTVDLPGLSLCGGDPLN---FIAEELED-----MLSNNLSNEKIFEFLETHVDK-----PTWKKSECTKTITSSILKALRKIPESSVQEYLKSKKEIIKRISNHKLENEIEILKSILNVASELNRDT--LTRIYDLLITEKIVSHQAL 3727
V+ +E+ R +G L +L+ + +S Q +G + DD+ DIP IW + A+ + + K IS E I K +LP + S LH ++ HK VG ++ ++ L W+ +P + FL L+FT C + L+ AEEL ++ +E+IF+++E ++D+ PT+ ++ T ++I+ + +K + I+ + + E E++ L ++ +L++ L +D L E+++S A
Sbjct: 1257 VEFTLERSQITRDHMGHLLYQLVQSEKLSKQDFFKGFSETLELADDMAIDIPHIWLYLAELVTPML--KGGGISMRELIVEFSKPLLPVGRAGVLLSEILHLLCRQMSHKKVGALWREADLSWKDFLPEGEDVHHFLLEQKLDFTE---SEGPCSSEALSKKELSAEELSQRLEKLIMEEKADDERIFDWVEANLDESQMSSPTFLRALMTAVCKAAIIADCSTFRVDTA--VIKQRVPILLKYLDSDTEKELQALYALQASIVKLDQPANLLRMFFDCLYDEEVISEDAF 1540
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Mouse
Match: Eif4g3 (eukaryotic translation initiation factor 4 gamma, 3 [Source:MGI Symbol;Acc:MGI:1923935]) HSP 1 Score: 147.132 bits (370), Expect = 8.939e-35 Identity = 125/418 (29.90%), Postives = 211/418 (50.48%), Query Frame = 2
Query: 1268 YDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQPRVISCQLPPE--SSSSQTNNERANNKKQLPNRQYSKYKNYNHKQTE-------RKQINLEISDNQVFIRSKSGYLPPQLRDKQKLLDSLEELK-----RNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFC---YKLAITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDS-KMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKF-NLMTKKKNVSNSQNI 2449
YDR +L+ ++ QKP +P ++ D I NQPR+ L P A+ +Q P + N ++++ RK I + + ++ ++++ + P Q RD D E +K R ++S LNK+TPQ F + +K++ L ++ +E + +I+ +F KA+ E ++SV YA C L + + P F+ LL +CQ FE +D+ ++ E K+K L + + + +LEE KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K LMTK+K Q +
Sbjct: 802 YDREFLLDIQFMPACIQKPEGLPPISDVVLDKI-NQPRLSMRTLDPRILPRGPDFTPAFADFPRQTPGGRGVPLLNVGPRRSQPGQRREPRKIITVSVKEDVHLRKAENAWKPSQKRDSHA--DDPESIKTQELFRKVRSILNKLTPQMFNQLMKQVSALTVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLVTLKVPMADKPGNTVNFRKLLLNRCQKEFE---KDKADDDVFEKKQKELEAASAPEERTRLHDELEEAKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLCNWVSRRADQ----GPKTIEQIHKEAKIEEQEEQRKVQQLMTKEKRRPGVQRV 1208 HSP 2 Score: 65.4698 bits (158), Expect = 6.449e-10 Identity = 67/296 (22.64%), Postives = 131/296 (44.26%), Query Frame = 2
Query: 2903 IIEKWVDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFENIF---KSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNHWET--FLKVNNLEFTVDLPGLSLCGGDPLN---FIAEELED-----MLSNNLSNEKIFEFLETHVDK-----PTWKKSECTKTITSSILKALRKIPESSVQEYLKSKKEIIKRISNHKLENEIEILKSILNVASELNRDT--LTRIYDLLITEKIVSHQAL 3727
++ V+ +E+ R +G L +L+ + +S Q +G + DD+ DIP IW + A+ + + K IS E I K +LP + S LH ++ HK VG ++ ++ L W+ +P + FL L+FT C + L+ AEEL ++ +E+IF+++E ++D+ PT+ ++ T ++I+ + +K + I+ + + E E++ L ++ +L++ L +D L E+++S A
Sbjct: 1435 FVKVGVEFTLERSQITRDHMGHLLYQLVQSEKLSKQDFFKGFSETLELADDMAIDIPHIWLYLAELVTPML--KGGGISMRELIVEFSKPLLPVGRAGVLLSEILHLLCRQMSHKKVGALWREADLSWKDFLPEGEDVHHFLLEQKLDFTES---EGPCSSEALSKKELSAEELSQRLEKLIMEEKADDERIFDWVEANLDESQMSSPTFLRALMTAVCKAAIIADCSTFRVDTA--VIKQRVPILLKYLDSDTEKELQALYALQASIVKLDQPANLLRMFFDCLYDEEVISEDAF 1723
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Mouse
Match: Eif4g1 (eukaryotic translation initiation factor 4, gamma 1 [Source:MGI Symbol;Acc:MGI:2384784]) HSP 1 Score: 132.88 bits (333), Expect = 1.804e-30 Identity = 116/406 (28.57%), Postives = 199/406 (49.01%), Query Frame = 2
Query: 1250 LKRKLIYDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQP-------RVISCQLPPESSSSQTNNERA----------NNKKQLPNRQYSKYKNYNHKQTERKQINLEIS-----DNQVFIRSKSGYLPPQLR---DKQKLLDSL-----EELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFC---YKLAITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNE-TDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQID-VKHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTL 2350
L+ K YDR +L+ + S QKP +P T+ D + P R+ P+ + S N R +LP + +Q RK+ IS ++ +++ + P R DK + + ++L R ++S LNK+TPQ F + +K++ L I+ +E + +I+ IF KA+ E +SV YA C L + T P F+ LL +CQ FE +D+ ++ E K+K ++E ++ + + +LEE A+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D++D+R+ NW R Q PKT+
Sbjct: 603 LEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELPRGPQAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFE---KDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQ----GPKTI 1000 HSP 2 Score: 53.5286 bits (127), Expect = 2.682e-6 Identity = 37/191 (19.37%), Postives = 87/191 (45.55%), Query Frame = 2
Query: 2918 VDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNIS-FFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNHWE--TFLKVNNLEFTV----DLPGLSLCGGDPLNFIAEELEDMLSNNLSNEKIFEFLETHVDK 3466
++ +E+ R +G L++LL +S+ Q +G + +D+ DIP +W + A+ + + + + F I K + P L +G K VG ++ ++ L W + + + +F+ +E+T+ + PG + +LE +L + SN+++F++++ ++++
Sbjct: 1277 IESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLA---FEELRRQLEKLLKDGGSNQRVFDWIDANLNE 1464
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Mouse
Match: Eif4g1 (eukaryotic translation initiation factor 4, gamma 1 [Source:MGI Symbol;Acc:MGI:2384784]) HSP 1 Score: 132.494 bits (332), Expect = 2.165e-30 Identity = 119/409 (29.10%), Postives = 196/409 (47.92%), Query Frame = 2
Query: 1250 LKRKLIYDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQP-------RVISCQLPPESSSSQTNNERA----------NNKKQLPNRQYSKYKNYNHKQTERKQINLEIS-----DNQVFIRSKSGYLPPQLR---DKQKLLDSL-----EELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFC---YKLAITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKD----KYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQID-VKHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTL 2350
L+ K YDR +L+ + S QKP +P T+ D + P R+ P+ + S N R +LP R + +Q RK+ IS ++ +++ + P R DK + + ++L R ++S LNK+TPQ F + +K++ L I+ +E + +I+ IF KA+ E +SV YA C L + T P F+ LL +CQ FE D +E EK E D A L+E++++A+D + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D++D+R+ NW R Q PKT+
Sbjct: 313 LEEKKRYDREFLLGFQFIFASMQKPEGLPHITDVVLDKANKTPLRQLDPSRLPGINCGPDFTPSFANLGRPALSNRGPPRGGPGGELP-RGPAGLGPRRSQQGPRKETRKIISSVIMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDD------DEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQ----GPKTI 709 HSP 2 Score: 54.299 bits (129), Expect = 1.614e-6 Identity = 58/283 (20.49%), Postives = 122/283 (43.11%), Query Frame = 2
Query: 2654 RSREQSPQVILANKYRNLESSAEASGADS--RSSTPPMIRPITKAFRIIEQEVTDEEMAE--IIFNDLLSTGNFDIKKWLTLRKVQPIIEKWVDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNIS-FFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNHWE--TFLKVNNLEFTV----DLPGLSLCGGDPLNFIAEELEDMLSNNLSNEKIFEFLETHVDK 3466
RSRE+ Q R S E G D R +T P + P A + E E + + E + ND+ +++ + + + ++ +E+ R +G L++LL +S+ Q +G + +D+ DIP +W + A+ + + + + F I K + P L +G K VG ++ ++ L W + + + +F+ +E+T+ + PG + L +LE +L + SN+++F++++ ++++
Sbjct: 898 RSRERPSQ---PEGLRKAASLTEDRGRDPVKREATLPPVSPPKAALSVDEVEKKSKAIIEEYLHLNDMKEAVQC-VQELASPSLLFIFVRLGIESTLERSTIAREHMGRLLHQLLCAGHLSTAQYYQGLYETLELAEDMEIDIPHVWLYLAELITPILQEDGVPMGELFREITKPLRPMGKATSLLLEILGLLCKSMGPKKVGMLWREAGLSWREFLAEGQDVGSFVAEKKVEYTLGEESEAPGQRTLAFEELR---RQLEKLLKDGGSNQRVFDWIDANLNE 1173
BLAST of Eukaryotic initiation factor-4G vs. UniProt/SwissProt
Match: sp|O43432|IF4G3_HUMAN (Eukaryotic translation initiation factor 4 gamma 3 OS=Homo sapiens OX=9606 GN=EIF4G3 PE=1 SV=2) HSP 1 Score: 147.902 bits (372), Expect = 2.988e-34 Identity = 126/418 (30.14%), Postives = 210/418 (50.24%), Query Frame = 2
Query: 1268 YDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQPRVISCQLPPE--SSSSQTNNERANNKKQLPNRQY-------SKYKNYNHKQTERKQINLEISDNQVFIRSKSGYLPPQLRDKQKLLDSLE-----ELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFC---YKLAITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDS-KMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKF-NLMTKKKNVSNSQNI 2449
YDR +L+ + QKP +P ++ D I NQP++ L P A+ +Q P + S+ ++ RK I + + ++ ++++ + P Q RD Q D E EL R ++S LNK+TPQ F + +K++ L ++ +E + +I+ +F KA+ E ++SV YA C L + + P F+ LL +CQ FE +D+ ++ E K+K L + + + +LEE KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K LMTK+K Q +
Sbjct: 624 YDREFLLDFQFMPACIQKPEGLPPISDVVLDKI-NQPKLPMRTLDPRILPRGPDFTPAFADFGRQTPGGRGVPLLNVGSRRSQPGQRREPRKIITVSVKEDVHLKKAENAWKPSQKRDSQA--DDPENIKTQELFRKVRSILNKLTPQMFNQLMKQVSGLTVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLVTLKVPMADKPGNTVNFRKLLLNRCQKEFE---KDKADDDVFEKKQKELEAASAPEERTRLHDELEEAKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLCNWVSRRADQ----GPKTIEQIHKEAKIEEQEEQRKVQQLMTKEKRRPGVQRV 1030 HSP 2 Score: 63.929 bits (154), Expect = 1.302e-8 Identity = 68/290 (23.45%), Postives = 130/290 (44.83%), Query Frame = 2
Query: 2918 VDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFE---NIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNHWET--FLKVNNLEFTVDLPGLSLCGGDPLN---FIAEELEDML-----SNNLSNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSV----QEYLKSKKEIIKRISNHKLENEIEILKSILNVASELNRDT--LTRIYDLLITEKIVSHQAL 3727
V+ +E+ R +G L +L+ + +S Q +G + DD+ DIP IW + A+ + + K IS E K +LP + S LH ++ HK VG ++ ++ L W+ +P + FL L+F + S C + L+ AEEL L + ++E+IF+++E ++D+ + + +++ KA I +SS +K + I+ + + E E++ L ++ +L++ L +D L E+++S A
Sbjct: 1263 VESTLERSQITRDHMGQLLYQLVQSEKLSKQDFFKGFSETLELADDMAIDIPHIWLYLAELVTPML--KEGGISMRELTIEFSKPLLPVGRAGVLLSEILHLLCKQMSHKKVGALWREADLSWKDFLPEGEDVHNFLLEQKLDF---IESDSPCSSEALSKKELSAEELYKRLEKLIIEDKANDEQIFDWVEANLDEIQMSSPTFLRALMTAVCKAA-IIADSSTFRVDTAVIKQRVPILLKYLDSDTEKELQALYALQASIVKLDQPANLLRMFFDCLYDEEVISEDAF 1546
BLAST of Eukaryotic initiation factor-4G vs. UniProt/SwissProt
Match: sp|Q80XI3|IF4G3_MOUSE (Eukaryotic translation initiation factor 4 gamma 3 OS=Mus musculus OX=10090 GN=Eif4g3 PE=1 SV=2) HSP 1 Score: 147.902 bits (372), Expect = 3.082e-34 Identity = 126/418 (30.14%), Postives = 211/418 (50.48%), Query Frame = 2
Query: 1268 YDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQPRVISCQLPPE--SSSSQTNNERANNKKQLPNRQYSKYKNYNHKQTE-------RKQINLEISDNQVFIRSKSGYLPPQLRDKQKLLDSLE-----ELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFC---YKLAITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDS-KMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKF-NLMTKKKNVSNSQNI 2449
YDR +L+ ++ QKP +P ++ D I NQPR+ L P A+ +Q P + N ++++ RK I + + ++ ++++ + P Q RD D E EL R ++S LNK+TPQ F + +K++ L ++ +E + +I+ +F KA+ E ++SV YA C L + + P F+ LL +CQ FE +D+ ++ E K+K L + + + +LEE KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K LMTK+K Q +
Sbjct: 619 YDREFLLDIQFMPACIQKPEGLPPISDVVLDKI-NQPRLSMRTLDPRILPRGPDFTPAFADFPRQTPGGRGVPLLNVGPRRSQPGQRREPRKIITVSVKEDVHLRKAENAWKPSQKRDSHA--DDPESIKTQELFRKVRSILNKLTPQMFNQLMKQVSALTVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLVTLKVPMADKPGNTVNFRKLLLNRCQKEFE---KDKADDDVFEKKQKELEAASAPEERTRLHDELEEAKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLCNWVSRRADQ----GPKTIEQIHKEAKIEEQEEQRKVQQLMTKEKRRPGVQRV 1025 HSP 2 Score: 65.4698 bits (158), Expect = 4.725e-9 Identity = 67/291 (23.02%), Postives = 129/291 (44.33%), Query Frame = 2
Query: 2918 VDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFENIF---KSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNHWET--FLKVNNLEFTVDLPGLSLCGGDPLN---FIAEELED-----MLSNNLSNEKIFEFLETHVDK-----PTWKKSECTKTITSSILKALRKIPESSVQEYLKSKKEIIKRISNHKLENEIEILKSILNVASELNRDT--LTRIYDLLITEKIVSHQAL 3727
V+ +E+ R +G L +L+ + +S Q +G + DD+ DIP IW + A+ + + K IS E I K +LP + S LH ++ HK VG ++ ++ L W+ +P + FL L+FT C + L+ AEEL ++ +E+IF+++E ++D+ PT+ ++ T ++I+ + +K + I+ + + E E++ L ++ +L++ L +D L E+++S A
Sbjct: 1257 VEFTLERSQITRDHMGHLLYQLVQSEKLSKQDFFKGFSETLELADDMAIDIPHIWLYLAELVTPML--KEGGISMRELIVEFSKPLLPVGRAGVLLSEILHLLCRQMSHKKVGALWREADLSWKDFLPEGEDVHHFLLEQKLDFTE---SEGPCSSEALSKKELSAEELSQRLEKLIMEEKADDERIFDWVEANLDESQMSSPTFLRALMTAVCKAAIIADCSTFRVDTA--VIKQRVPILLKYLDSDTEKELQALYALQASIVKLDQPANLLRMFFDCLYDEEVISEDAF 1540
BLAST of Eukaryotic initiation factor-4G vs. UniProt/SwissProt
Match: sp|Q6NZJ6|IF4G1_MOUSE (Eukaryotic translation initiation factor 4 gamma 1 OS=Mus musculus OX=10090 GN=Eif4g1 PE=1 SV=1) HSP 1 Score: 131.724 bits (330), Expect = 2.428e-29 Identity = 86/253 (33.99%), Postives = 147/253 (58.10%), Query Frame = 2
Query: 1619 EELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFC---YKLAITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNE-TDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQID-VKHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTL 2350
++L R ++S LNK+TPQ F + +K++ L I+ +E + +I+ IF KA+ E +SV YA C L + T P F+ LL +CQ FE +D+ ++ E K+K ++E ++ + + +LEE A+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D++D+R+ NW R Q PKT+
Sbjct: 762 QDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFE---KDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFAKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRQSNWVPRRGDQ----GPKTI 1006
BLAST of Eukaryotic initiation factor-4G vs. UniProt/SwissProt
Match: sp|Q04637|IF4G1_HUMAN (Eukaryotic translation initiation factor 4 gamma 1 OS=Homo sapiens OX=9606 GN=EIF4G1 PE=1 SV=4) HSP 1 Score: 129.798 bits (325), Expect = 1.096e-28 Identity = 86/253 (33.99%), Postives = 146/253 (57.71%), Query Frame = 2
Query: 1619 EELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFC---YKLAITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNE-TDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTL 2350
++L R ++S LNK+TPQ F + +K++ L I+ +E + +I+ IF KA+ E +SV YA C L + T P F+ LL +CQ FE +D+ ++ E K+K ++E ++ + + +LEE A+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D++D+R NW R Q PKT+
Sbjct: 758 QDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFE---KDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQ----GPKTI 1002 HSP 2 Score: 64.3142 bits (155), Expect = 1.004e-8 Identity = 43/194 (22.16%), Postives = 89/194 (45.88%), Query Frame = 2
Query: 2903 IIEKWVDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNIS-FFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNHWE--TFLKVNNLEFTV----DLPGLSLCGGDPLNFIAEELEDMLSNNLSNEKIFEFLETHV 3460
+ V+ +E+ R +G L++LL +S+ Q +G + +D+ DIP +W + A+ + + + + F I K + P A L +G K VG ++ ++ L W++ +P + F+ +E+T+ + PG + LN +LE +L SN+++F+++E ++
Sbjct: 1278 FVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELN---RQLEKLLKEGSSNQRVFDWIEANL 1468
BLAST of Eukaryotic initiation factor-4G vs. UniProt/SwissProt
Match: sp|B9FXV5|IF4G_ORYSJ (Eukaryotic translation initiation factor 4G OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0555200 PE=2 SV=2) HSP 1 Score: 119.398 bits (298), Expect = 1.719e-25 Identity = 89/256 (34.77%), Postives = 146/256 (57.03%), Query Frame = 2
Query: 1601 KLLDSLEELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLAITYTN------NPAFKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRD--SYDSDAMLALCKLLETCGKQID-VKHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRH-VQQEKQIN 2338
K+ D E +R +K+ LNK+TPQNF + ++++ +NI+N ++ +I++IF KA+ E + YA FC+ LA + FK LL KCQ+ FE +++ E + E +E + +T + + EE+ +A+ + GNIR IGEL+ +L +++ CI +LL + + D + + ALCKL+ T G+ ID K KE M+ Y + +LS S L R+RF L+D ID+R+ W R V+ K+I+
Sbjct: 1174 KVSDEEEAKQRQLKAILNKLTPQNFEKLFEKVKEVNIDNVATLTGVISQIFDKALMEPTFCEMYANFCFHLAGALPDFSEDNEKITFKRLLLNKCQEEFERGEREEAEADKTE-EEGEIKQT--------KEEREEKRIRARRRMLGNIRLIGELYKKRMLTERIMHECIKKLLGNYQNPDEENIEALCKLMSTIGEMIDHAKAKEHMDAYFDIMLKLSTSQQLSSRVRFMLRDSIDLRKNKWQQRRKVEGPKKID 1420
BLAST of Eukaryotic initiation factor-4G vs. TrEMBL
Match: D5JG54 (Eukaryotic initiation factor-4G OS=Dugesia japonica OX=6161 GN=eIF4G PE=2 SV=1) HSP 1 Score: 823.157 bits (2125), Expect = 0.000e+0 Identity = 419/566 (74.03%), Postives = 485/566 (85.69%), Query Frame = 2
Query: 2129 MLALCKLLETCGKQIDVKHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKFNLMTKKKNVSN-SQNIARRNIQ----KNVSIKNLSCNSHKVDEKTVLGPVLGNWATGSGSKSKSHEAEGWTPVSQKPKGGAMRYTSNSRSREQSPQVILANKYRNLESSAEASGADSRSSTPPMIRPITKAFRIIEQEVTDEEMAEIIFNDLLSTGNFDIKKWLTLRKVQPIIEKWVDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKSLKWEKIVPNHWETFLKVNNLEFTVDLPGLSLCGGDPLNFIAEELEDMLSNNLSNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSVQEYLKSKKEIIKRISNHKLENEIEILKSILNVASELNRDTLTRIYDLLITEKIVSHQALKDYCDNESLKSENNFKLTNSLLSDILYL 3811
MLALCKLLETCGKQID+KHKE MN +I+ I LS+S+DLEKRIRFKL+DIIDMR R+WTLRHVQQEKQINPKTLEEQK EAAAEA+A +K M KK+N N SQN RRN+ KNVSIKNLSC+ H VDEKTVLGP G+W GSGSKSKSHEAE W PVS K KGG +RYTSNSRSREQSPQVIL NKYRNLESSAEASGADSRSSTPPMI I+KA+R++E E++DEE A+IIFNDLLSTG+FDI W LR + +IEKW+DDMMEKD SCRR IGD+ NRLLI+KII+++QI++GT+ FVSSIDDLICDIPK+W FFAQFLERFI DKNSNI FFE+IFKSILPN DVA CFS ALH+AS RLGHKIVGQVFH+SL W IVP++WE F+K NNLEFTV L LS CGGDPL+FI EEL DMLS+N SNE IF+FLE VDKPTWKKSECT+TITSSILKA++K+PESSV+EY K+KKEIIKRISNHK ENE+EILK+ILN+AS+LN++TLT IYDLLITE+I+S + LK Y ++ESLKSE+NFKLT+ +LS ++Y+
Sbjct: 1 MLALCKLLETCGKQIDMKHKEQMNLFINKIYELSKSIDLEKRIRFKLRDIIDMRVRDWTLRHVQQEKQINPKTLEEQKEEAAAEASANALKLAAMNKKRNAQNPSQNNPRRNVNHAGNKNVSIKNLSCHRHTVDEKTVLGPAGGSWIYGSGSKSKSHEAEVWIPVSLKTKGGTIRYTSNSRSREQSPQVILGNKYRNLESSAEASGADSRSSTPPMIPLISKAYRVVEPEMSDEERADIIFNDLLSTGSFDINSWKKLRTIPVVIEKWIDDMMEKDASCRRTIGDNFNRLLINKIITTEQIIDGTKSFVSSIDDLICDIPKLWNFFAQFLERFILDKNSNIKFFESIFKSILPNQDVANCFSAALHDASDRLGHKIVGQVFHRSLNWNNIVPDNWEQFIKQNNLEFTVYLCPLSSCGGDPLHFIEEELADMLSSNSSNENIFDFLEXXVDKPTWKKSECTRTITSSILKAIKKMPESSVREYFKNKKEIIKRISNHKPENEMEILKAILNIASDLNKETLTLIYDLLITEEIISLEVLKTYLEDESLKSESNFKLTHPILS-VMYV 565
BLAST of Eukaryotic initiation factor-4G vs. TrEMBL
Match: A0A1I8I699 (MI domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 228.024 bits (580), Expect = 4.038e-57 Identity = 209/752 (27.79%), Postives = 353/752 (46.94%), Query Frame = 2
Query: 1571 YLPPQLRDKQKLLDSLE--ELKRNIKSNLNKITPQNFPECVXXXXXX-XXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLAITYTNNP----------AFKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAML-----ALCKLLETCGKQIDVKH--KESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKFNLMTKKKNVSN------SQNIAR----------RNIQKNVSIKNLSCNSHKVDE-KTVLGPVLGNWATGSGSKSKSHEAEGWTP--VSQK--PKGGAMRYTSNSRSREQSPQVILANKYRNLESSAEASGADSRSSTPPMI--RPITKAFRIIEQEVTDEEMAEIIFNDLLSTGNF-DIKKWLTLRKVQPIIEKWVDD-----MMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFENIFKSILPN-NDVAL------CFSTALHEASSRLGHKIVGQVFHKS-LKWEKI--VPNH-WETFLKVNNLEFTVDLPGLSLCGGDP--LNFIAEELEDMLSN--------NLSNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSVQEYLKSKKEIIKRISNHKLENE 3616
Y+P L+ K + L+ E++RNI+S LNKI PQNF C ++ +I + + ++ ++ IF KA +E++YS +A+ C +LA N P F+ LLKKCQ+MFETPL++Q+E +W+EK+ E K E + +EQ+KKAKD+Y+GNIRFIGELF+ ++L +I CIV L+ + S +M LC+LL+T GK+++ ++ K +++Y I +L S ++E ++RF+L+DI++MR+RNW R ++ P T EE + +A + + + T+ Q +A R QK + K + +S + + +T LGP ++ + K G TP SQK + A R TS S PQ + L +S + + S P P + I ++V + + I+ L S + D K P I +V+ + E R + G L LL +++SS+ ++EG + DD + D P + + + F+ S +S + + I + D + S ALH AS RLGH VG F ++ + W + +P F+ N LEFT+ LS P L+ + E +E + N +L +E++ +E VDK + E + + S+ + + + + +L K +++ I H+ + +
Sbjct: 8 YVPGFLQKKPDDQEQLKKVEIERNIRSILNKIAPQNFESCFSQLTTTCDIGSIQGLNACVDIIFDKATREKSYSSVFAEICKRLAFLKVNFPEQSQENKQFIGFRSLLLKKCQEMFETPLKEQMEQKGAQWQEKIDAEEKEDKKKALEEERDEQMKKAKDQYYGNIRFIGELFMKDILTANIIHQCIVSQLKSDHSSKSMFTESLECLCELLKTVGKKLESENNAKARIDQYFERIKKLINSKEIESKVRFRLQDIVEMRQRNWERREMEIRLHERPMTKEELEQKARQDEESWAQQLQQQTRGGRQGGFSRPPAGQTLAANSKWTPVTTTRKAQKMDARKMVIGSSQEQGQSQTRLGPSRPSFVAKARPSGKDDGGRG-TPRLGSQKNSRESSASRGTSREASLHLDPQQLQQPAVAALTASGKMPRSQSSQELPARAVESPRSVEPPPIPRDVLERKSKNIMMEWLGSLCSLEDTKSNFIEISRHPEISAFVEVNAEHLLNENSKELREKGGQLLFELLHSELLSSKDLIEGFSPIMEMADDFLSDFPLLSSYLGEIFSSFVQKAASYLSCVQQLVLRIPADLRDSSRRPLRGEVVSRALHLASGRLGHDRVGAAFREAGMAWHSLLELPQADAADFVAANKLEFTLHETTLS---PSPIALDVVGERIEQKIGNADGDASAKDLLSEELIALIEKEVDKKQMESREFIRVLVRSVCRGCTQSKKLNKPLFLLVKPVLMRHIETHEQQQQ 755
BLAST of Eukaryotic initiation factor-4G vs. TrEMBL
Match: A0A267H8S5 (MI domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig018935g1 PE=4 SV=1) HSP 1 Score: 224.172 bits (570), Expect = 8.789e-57 Identity = 205/749 (27.37%), Postives = 350/749 (46.73%), Query Frame = 2
Query: 1568 GYLPPQLRDKQKLLDSLEELKRNIKSNLNKITPQNFPECVXXXXXX-XXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLAITYTNNP----------AFKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLA-----LCKLLETCGKQIDVKH--KESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKFNLMTKKKNVSN------SQNIAR----------RNIQKNVSIKNLSCNSHKVDE-KTVLGPVLGNWATGSGSKSKSHEAEGWTP--VSQK--PKGGAMRYTSNSRSREQSPQVILANKYRNLESSAEASGADSRSSTPPMI--RPITKAFRIIEQEVTDEEMAEIIFNDLLSTGNF-DIKKWLTLRKVQPIIEKWVDD-----MMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFENIFKSILPN-NDVAL------CFSTALHEASSRLGHKIVGQVFHKS-LKWEKI--VPNH-WETFLKVNNLEFTVDLPGLSLCGGDPLNFIAEELEDMLSN--------NLSNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSVQEYLKSKKEIIKRISNHKLENE 3616
GYL + D+++L E++RNI+S LNKI PQNF C ++ +I + + ++ ++ IF KA +E++YS +A+ C +LA N P F+ LLKKCQ+MFETPL++Q+E +W+EK+ E K E + +EQ+KKAKD+Y+GNIRFIGELF+ ++L +I CIV L+ + S +M L +LL+T GK+++ ++ K +++Y I +L S ++E ++RF+L+DI++MR+RNW R ++ P T +E + +A + + + T+ Q +A R QK + K + +S + + +T LGP ++ + K G TP SQK + A R TS S PQ + L +S + + S P P + I ++V + + I+ L S + D K P I +V+ + E R + G L LL +++SS+ ++EG + DD + D + + + F+ S +S + + I + D + S ALH AS RLGH VG F ++ + W + +P F+ N LEFT+ L L L+ + E +E + N +L +E++ +E VDK + E + + S+ + + + + +L K +++ I H+ + +
Sbjct: 11 GYLQKKPDDQEQLKKV--EIERNIRSILNKIAPQNFESCFSQLTTTCDIGSIQGLNACVDIIFDKATREKSYSSVFAEICKRLAFLKVNFPEQSQENKQFIGFRSLLLKKCQEMFETPLKEQMEQKGAQWQEKIDAEEKEDKKKALEEERDEQMKKAKDQYYGNIRFIGELFMKDILTANIIHQCIVSQLKSDHSSKSMFTESLECLFELLKTVGKKLESENNAKARIDQYFERIKKLINSKEIESKVRFRLQDIVEMRQRNWERREMEIRLHERPMTKKELEQKARQDEESWAQQLQQQTRGGRQGGFSRPPAGQTLAANSKWTPVTTTRKAQKMDARKMVIGSSQEQGQSQTRLGPSRPSFVAKARPSGKDDGGRG-TPRLGSQKNSRESSASRGTSREASLHLDPQQLQQPAVAALTASGKMPRSQSSQELPARAVESPRSVEPPPIPRDVLERKSKNIMMEWLGSLCSLEDTKSNFIEISRHPEISAFVEVNAEHLLNESSKELREKGGQLLFELLHSELLSSKDLIEGFSPIMEMADDFLSDFSLLSSYLGEIFSSFVQKAASYLSCVQQLVLRIPADLRDSSRRPLRGEVVSRALHLASGRLGHDRVGAAFREAGMAWHSLLELPQADAADFVAANKLEFTLHETTL-LPSPIALDVVGERIEQKIGNADGDASAKDLLSEELIALIEKEVDKKQMESREFIRVLARSVCRGCTQSKKLNKPLFLLVKPVLMRHIETHEQQQQ 755
BLAST of Eukaryotic initiation factor-4G vs. TrEMBL
Match: A0A1I8G5Z3 (MIF4G domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 220.705 bits (561), Expect = 5.724e-55 Identity = 197/736 (26.77%), Postives = 346/736 (47.01%), Query Frame = 2
Query: 1568 GYLPPQLRDKQKLLDSLEELKRNIKSNLNKITPQNFPECVXXXXXX-XXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLAITYTNNP----------AFKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAML-----ALCKLLETCGKQIDVKH--KESMNKYISDISRLSQSVDLEKRIRFKLKDI-----IDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKFNLMTKKKNVSNSQNIARRNIQKNVSIKNLSCNSHKVDEKTVLGPVLGNWATGSGSKSKSHEAEGWTPVSQKPKGGAMRYTSNSRSREQSPQVILANKYRNLESSAEASGADSRS-STPPMIRPITKAFRIIE-----QEVTDEEMAEIIFNDLLSTGNF-DIKKWLTLRKVQPIIEKWVDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFENIFKSILPN-NDVAL------CFSTALHEASSRLGHKIVGQVFHKS-LKWEKI--VPNH-WETFLKVNNLEFTVDLPGLSLCGGDP--LNFIAEELEDMLSN--------NLSNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSVQEYLKSKKEIIKRISNHKLENEIE 3622
GYL + D+++L E++RNI+S LNKI PQNF C ++ +I + + ++ ++ IF KA +E++YS +A+ C +LA N P F+ LLKKCQ+MFETPL++Q+E +W+EK+ E K E + +EQ+KKAKD+Y+GNIRFIGELF+ ++L +I CIV L+ + S +M LC+LL+T GK+++ ++ K +++Y I +L S ++E ++RF+L+DI E +W +Q+ +T ++ + T+ N +K V+ ++ + + +K V + + + +T LGP ++ + K G TP K S SRE SP + + ++ ASG RS S+ + ++ R +E ++V + + I+ L S + D K P I +V+ + G L LL +++SS+ ++EG + DD + D P + + + F+ S +S + + I + D + S ALH AS RLGH VG F ++ + W + +P F+ N LEFT+ LS P L+ + E +E + N +L +E++ +E VDK + E + + S+ + + + + +L K +++ I H+ + +++
Sbjct: 11 GYLQKKPDDQEQLKKV--EIERNIRSILNKIAPQNFESCFSQLTTTCDIGSIQGLNACVDIIFDKATREKSYSSVFAEICKRLAFLKVNFPEQSQENKQFIGFRSLLLKKCQEMFETPLKEQMEQKGAQWQEKIDAEEKEDKKKALEEERDEQMKKAKDQYYGNIRFIGELFMKDILTANIIHQCIVSQLKSDHSSKSMFTESLECLCELLKTVGKKLESENNAKARIDQYFERIKKLINSKEIESKVRFRLQDIELEQKARQDEESWA-------QQLQQQTRGGRQGGFSRPPAGQTLAAN--SKWTPVTTTRKAQKMDARKMV----IGSSQEQGQSQTRLGPSRPSFVAKARPSGKDDGGRG-TPRLGSQKNSRESSASRGTSREASPHLDPQQLQQPAVAALAASGKMPRSQSSQELPARAVESPRSVEPPPIPRDVLERKSKNIMMEWLGSLCSLEDTKSNFIEISRHPEISAFVEQRASGEG------GQLLFELLHSELLSSKDLIEGFSPIMEMADDFLSDFPLLSSYLGEIFSSFVQKAASYLSCVQQLVLRIPADLRDSSRRPLRGEVVSRALHLASGRLGHDRVGAAFREAGMAWHSLLELPQADAADFVAANKLEFTLHETTLS---PSPIALDVVGERIEQKIGNADGDASAKDLLSEELIALIEKEVDKKQMESREFIRVLVRSVCRGCTQSKKLNKPLFLLVKPVLMRHIETHEQQQQVD 721
BLAST of Eukaryotic initiation factor-4G vs. TrEMBL
Match: A0A267HB46 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig013413g3 PE=4 SV=1) HSP 1 Score: 216.083 bits (549), Expect = 9.160e-53 Identity = 141/411 (34.31%), Postives = 228/411 (55.47%), Query Frame = 2
Query: 1196 SSSNYHLPVNKKESDETILKRKLIYDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQPRVISCQLPPESSSS---------QTNNERANNKKQL-----PNRQYSKYKNYNHKQTERKQINLEISDNQVFI-RSKSGYLPPQLRDKQKLLDSLE--ELKRNIKSNLNKITPQNFPECVXXXXXX-XXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLAITYTNNP----------AFKGQLLKKCQDMFETPLQDQLENVRNEWKEKV-LNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAML-----ALCKLLETCGKQIDVKH--KESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQ 2320
+S Y ESD+T L++KL YDR +L+ ++ S Q+P +P DI PR S Q PP + Q N + K + + K + Q+ +E+ ++V + +S S Y+P L+ K + L+ E++RNI+S LNKI PQNF C ++ +I + + ++ ++ IF KA +E++YS +A+ C +LA N P F+ LLKKCQ+MFETPL++Q+E +W+EK+ E + K KAL E + +EQ+KKAKD+Y+GNIRFIGELF+ ++L +I CIV L+ + S +M LC+LL+T GK+++ ++ K +++Y I +L S ++E ++RF+L+DI++MR+RNW R ++
Sbjct: 473 ASGKYEFKPEPGESDKTTLEKKLKYDRGFLLSLQFTDLSNQRPDELPG------DIPELLPRSDSQQQPPRAVRGFDFTPSFVVQPNYVAKDKGKSIYGSHRGSGGRGGGGGGPPKPAPKPQLVIEVRKDKVDLHKSDSAYVPGFLQKKPDDQEQLKKVEIERNIRSILNKIAPQNFESCFSQLTTTCDIGSIQGLNACVDIIFDKATREKSYSSVFAEICKRLAFLKVNFPEQSQENKQFIGFRSLLLKKCQEMFETPLKEQMEQKGAQWQEKIDAEEKEDKKKAL-EEERDEQMKKAKDQYYGNIRFIGELFMKDILTANIIHQCIVSQLKSDHSSKSMFTESLECLCELLKTVGKKLESENNAKARIDQYFERIKKLINSKEIESKVRFRLQDIVEMRQRNWERREME 876
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Cavefish
Match: eif4g3a (eukaryotic translation initiation factor 4 gamma, 3a [Source:ZFIN;Acc:ZDB-GENE-120810-2]) HSP 1 Score: 142.895 bits (359), Expect = 1.023e-33 Identity = 114/384 (29.69%), Postives = 198/384 (51.56%), Query Frame = 2
Query: 1268 YDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQP-----RVISCQLPPESSSSQTNNERANNKKQLPNRQYSKYKNYNHKQTERKQI--NLEISDNQVFIRSKSGYLPPQLRDK-------QKLLDSLEELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA---ITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDS-KMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTL 2350
YDR +L+ + QKP +P ++ D I NQ R++ +L A+ +QLP + + ++ ++I N+ ++D+ ++++ + P R+ QK D L + ++S LNK+TPQ F +K++ L I +E + +I+ +F KA+ E ++SV YA C+ LA + + P F+ LL +CQ FE +D++++ E K+K L+ S K + +LEE KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + ++ + RIRF L+D+ID+R NW R +Q PKT+
Sbjct: 706 YDREFLLGFQFMPACVQKPEGLPPISDVVLDKI-NQTKVPLIRMVDPRL--MIRGPDFTPAFADFTRQLPGMRGPPLLSLGARRPPPRKIIMNVSVNDDVQLKKAENAWKPGMKREAATEDQETQKTQDVL----KKVRSILNKLTPQMFTSLMKQVTDLTINTEERLKGVIDLVFEKAIDEPSFSVAYANMCHCLATLKVPMADKPGTTVNFRKLLLNRCQKEFE---RDKMDDAAFEKKQKELDSASSVTEKERLQEELEEAKDKARRRSTGNIKFIGELFKLRMLTEPIMHDCVVKLLKN-HDDESLECLCRLLTTIGKDLDFEKAKPRMDQYFHQMEKIVKERKTSSRIRFMLQDVIDLRLHNWVSRRAEQ----GPKTI 1074 HSP 2 Score: 58.5362 bits (140), Expect = 6.377e-8 Identity = 61/280 (21.79%), Postives = 122/280 (43.57%), Query Frame = 2
Query: 2930 MEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNI-SFFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVP--NHWETFLKVNNLEFTVDLPGLSLCGGDPLNFIAEELEDMLSNNL-----SNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSVQ---EYLKSKKEIIKRISNHKLENEIEILKSILNVASELNR--DTLTRIYDLLITEKIVSHQAL 3727
+E+ R +G L +L I+ Q+ G + DD+ DIP IW + A+ L + + + F + K +LP + S LH + H+ VG ++ +S L W + ++F+ L+FT S + EEL L N L ++E+IF+++E ++D+ S + + +++ KA K +S + ++ + ++ + N E +++ L ++ + L++ + L +D L E ++S A
Sbjct: 1308 LERSQISREHMGHLLYQLTQQSILPRTQLYTGFADTLEMADDMAIDIPHIWLYLAELLCPLLKEGGIPMRELFSELSKPLLPVGRAGVLLSEILH-LLCKQSHRKVGALWRESGLNWSDFLSEDEDLQSFISEQKLQFTQSDSSSSPSSPSKPSLPPEELHTQLENLLLVDMATDEQIFDWVEANLDESQISSSLFLRALMTAVCKAAVKDENTSCRVDTAIIQKRLPVLHKYFNADTERQLQALYALQALIVTLDQPPNLLRMFFDCLYDEDVISEDAF 1586
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Cavefish
Match: eif4g3b (eukaryotic translation initiation factor 4 gamma 3 [Source:NCBI gene;Acc:103031287]) HSP 1 Score: 141.354 bits (355), Expect = 3.085e-33 Identity = 156/562 (27.76%), Postives = 267/562 (47.51%), Query Frame = 2
Query: 1028 ILLESKTPLSVKSFERMEFEEFDLQNKAPESSIRLDIEEDVATCSSEDEVHIDLKLSSSNYHLPVNKKESDETILKRKLIYDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISN--------QPRVISCQLPPESSSSQTNNERANNKKQLPNRQYSKYKNYNHKQTERKQI--NLEISDNQVFIRSKSGYLPPQLRDKQKLLDSLEELK-----RNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA---ITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVK-KAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKFN--LMTKKKNVSNSQNIARRN---IQKNVSIKNLS----CNSHKVDEKTVLGPVLG-NWATGS--GSKSKSHEAEGWTP 2605
+ +E ++ + E E E L+N PE + D E+V T + LK +S+N + K E + K Y+R +L+ + QKP +P ++ D I+ PRVIS A+ + +P + + N ++ ++I N+ ++D ++++ + P RD +D E LK R ++S LNK+TPQ F + +K++ L I+ +E + +I+ +F KA+ E ++SV Y C LA + + P F+ LL +CQ FE +D++++ E K++ L S + + E+ K KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K + L++K K Q N + KN + S + +D+K LGP NW GS G+ +K+ E++ P
Sbjct: 590 VCVERRSSTAAPVEENGEQEAEPLRN-GPEHTPEADSSEEVPTPA--------LKEASTNA---IQKPE----LKNGKRQYNRDFLLGFQFMPACVQKPEGLPPISDVVLDKINQNKLPLRQVDPRVIS-------RGPDFTPAFADFGRMIPGSRGAPLLNVGLRRPPPRKIIMNVSVNDEVQLKKAENAWKPGMKRDA--AIDDPENLKTQELFRKVRSILNKLTPQMFNQLMKQVTDLTIDTEERLKGVIDLVFEKAIDEPSFSVAYGNMCSCLATLKVPMADKPTSTVNFRKLLLNRCQKEFE---KDKMDDDVFEKKQRELEAASSATERERLQEELEEAKDKARRRSIGNIKFIGELFKLRMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLHNWVSRRADQ----GPKTIEQIHKEAKIEEQEEQRKVHQQLLSKDKRRPVLQREETWNTVPVPKNSRTIDPSKIPKISKPPMDDKIQLGPRAQLNWMKGSSGGAGAKASESDVLRP 1118 HSP 2 Score: 77.0258 bits (188), Expect = 1.559e-13 Identity = 67/290 (23.10%), Postives = 132/290 (45.52%), Query Frame = 2
Query: 2918 VDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNI-SFFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNHWE--TFLKVNNLEFTVDLPGLSLCGGDPLNFIA----------EELEDMLSNNL-SNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSVQ---EYLKSKKEIIKRISNHKLENEIEILKSI--LNVASELNRDTLTRIYDLLITEKIVSHQAL 3727
V+ +E+ R +G ++LL +S Q +G + DD+ DIP IW + A+ + + + ++ F K +LP + FS LH ++ H+ VG ++ S L W +P + +F+ L+FT LS C P ++ ++LE +L +L S+E+IF+++E ++D+ + + +++ KA K+ SS + ++ + ++ + N E +++ L ++ L V E + L +D L E ++S A
Sbjct: 1287 VESTLERSQITRDHMGQLFHQLLQAGSLSKPQFFKGFSETLELADDMAIDIPHIWLYLAELVNPVLREGGISMRELFSEFSKPLLPVGRAGILFSEILHLLCKQMSHRKVGALWKDSGLSWADFLPETEDVNSFITEQKLDFT-----LSDCSS-PAAAVSNRMLSPEELNKQLEKLLLEDLASDEQIFDWVEANLDESEMSSPPFLRALMTAVCKAAVKLESSSYRVDTAIIQKRLPVLHKYLNSDTERQLQALYALQALIVKLEQPPNLLRMFFDCLYDEDVISEDAF 1570
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Cavefish
Match: eif4g3b (eukaryotic translation initiation factor 4 gamma 3 [Source:NCBI gene;Acc:103031287]) HSP 1 Score: 140.969 bits (354), Expect = 4.810e-33 Identity = 156/562 (27.76%), Postives = 267/562 (47.51%), Query Frame = 2
Query: 1028 ILLESKTPLSVKSFERMEFEEFDLQNKAPESSIRLDIEEDVATCSSEDEVHIDLKLSSSNYHLPVNKKESDETILKRKLIYDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISN--------QPRVISCQLPPESSSSQTNNERANNKKQLPNRQYSKYKNYNHKQTERKQI--NLEISDNQVFIRSKSGYLPPQLRDKQKLLDSLEELK-----RNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA---ITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVK-KAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKFN--LMTKKKNVSNSQNIARRN---IQKNVSIKNLS----CNSHKVDEKTVLGPVLG-NWATGS--GSKSKSHEAEGWTP 2605
+ +E ++ + E E E L+N PE + D E+V T + LK +S+N + K E + K Y+R +L+ + QKP +P ++ D I+ PRVIS A+ + +P + + N ++ ++I N+ ++D ++++ + P RD +D E LK R ++S LNK+TPQ F + +K++ L I+ +E + +I+ +F KA+ E ++SV Y C LA + + P F+ LL +CQ FE +D++++ E K++ L S + + E+ K KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K + L++K K Q N + KN + S + +D+K LGP NW GS G+ +K+ E++ P
Sbjct: 692 VCVERRSSTAAPVEENGEQEAEPLRN-GPEHTPEADSSEEVPTPA--------LKEASTNA---IQKPE----LKNGKRQYNRDFLLGFQFMPACVQKPEGLPPISDVVLDKINQNKLPLRQVDPRVIS-------RGPDFTPAFADFGRMIPGSRGAPLLNVGLRRPPPRKIIMNVSVNDEVQLKKAENAWKPGMKRDA--AIDDPENLKTQELFRKVRSILNKLTPQMFNQLMKQVTDLTIDTEERLKGVIDLVFEKAIDEPSFSVAYGNMCSCLATLKVPMADKPTSTVNFRKLLLNRCQKEFE---KDKMDDDVFEKKQRELEAASSATERERLQEELEEAKDKARRRSIGNIKFIGELFKLRMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLHNWVSRRADQ----GPKTIEQIHKEAKIEEQEEQRKVHQQLLSKDKRRPVLQREETWNTVPVPKNSRTIDPSKIPKISKPPMDDKIQLGPRAQLNWMKGSSGGAGAKASESDVLRP 1220 HSP 2 Score: 77.0258 bits (188), Expect = 1.466e-13 Identity = 67/290 (23.10%), Postives = 132/290 (45.52%), Query Frame = 2
Query: 2918 VDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNI-SFFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNHWE--TFLKVNNLEFTVDLPGLSLCGGDPLNFIA----------EELEDMLSNNL-SNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSVQ---EYLKSKKEIIKRISNHKLENEIEILKSI--LNVASELNRDTLTRIYDLLITEKIVSHQAL 3727
V+ +E+ R +G ++LL +S Q +G + DD+ DIP IW + A+ + + + ++ F K +LP + FS LH ++ H+ VG ++ S L W +P + +F+ L+FT LS C P ++ ++LE +L +L S+E+IF+++E ++D+ + + +++ KA K+ SS + ++ + ++ + N E +++ L ++ L V E + L +D L E ++S A
Sbjct: 1389 VESTLERSQITRDHMGQLFHQLLQAGSLSKPQFFKGFSETLELADDMAIDIPHIWLYLAELVNPVLREGGISMRELFSEFSKPLLPVGRAGILFSEILHLLCKQMSHRKVGALWKDSGLSWADFLPETEDVNSFITEQKLDFT-----LSDCSS-PAAAVSNRMLSPEELNKQLEKLLLEDLASDEQIFDWVEANLDESEMSSPPFLRALMTAVCKAAVKLESSSYRVDTAIIQKRLPVLHKYLNSDTERQLQALYALQALIVKLEQPPNLLRMFFDCLYDEDVISEDAF 1672
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Cavefish
Match: eif4g3b (eukaryotic translation initiation factor 4 gamma 3 [Source:NCBI gene;Acc:103031287]) HSP 1 Score: 140.969 bits (354), Expect = 5.060e-33 Identity = 156/562 (27.76%), Postives = 267/562 (47.51%), Query Frame = 2
Query: 1028 ILLESKTPLSVKSFERMEFEEFDLQNKAPESSIRLDIEEDVATCSSEDEVHIDLKLSSSNYHLPVNKKESDETILKRKLIYDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISN--------QPRVISCQLPPESSSSQTNNERANNKKQLPNRQYSKYKNYNHKQTERKQI--NLEISDNQVFIRSKSGYLPPQLRDKQKLLDSLEELK-----RNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA---ITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVK-KAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKFN--LMTKKKNVSNSQNIARRN---IQKNVSIKNLS----CNSHKVDEKTVLGPVLG-NWATGS--GSKSKSHEAEGWTP 2605
+ +E ++ + E E E L+N PE + D E+V T + LK +S+N + K E + K Y+R +L+ + QKP +P ++ D I+ PRVIS A+ + +P + + N ++ ++I N+ ++D ++++ + P RD +D E LK R ++S LNK+TPQ F + +K++ L I+ +E + +I+ +F KA+ E ++SV Y C LA + + P F+ LL +CQ FE +D++++ E K++ L S + + E+ K KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K + L++K K Q N + KN + S + +D+K LGP NW GS G+ +K+ E++ P
Sbjct: 717 VCVERRSSTAAPVEENGEQEAEPLRN-GPEHTPEADSSEEVPTPA--------LKEASTNA---IQKPE----LKNGKRQYNRDFLLGFQFMPACVQKPEGLPPISDVVLDKINQNKLPLRQVDPRVIS-------RGPDFTPAFADFGRMIPGSRGAPLLNVGLRRPPPRKIIMNVSVNDEVQLKKAENAWKPGMKRDA--AIDDPENLKTQELFRKVRSILNKLTPQMFNQLMKQVTDLTIDTEERLKGVIDLVFEKAIDEPSFSVAYGNMCSCLATLKVPMADKPTSTVNFRKLLLNRCQKEFE---KDKMDDDVFEKKQRELEAASSATERERLQEELEEAKDKARRRSIGNIKFIGELFKLRMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLHNWVSRRADQ----GPKTIEQIHKEAKIEEQEEQRKVHQQLLSKDKRRPVLQREETWNTVPVPKNSRTIDPSKIPKISKPPMDDKIQLGPRAQLNWMKGSSGGAGAKASESDVLRP 1245 HSP 2 Score: 77.0258 bits (188), Expect = 1.434e-13 Identity = 67/290 (23.10%), Postives = 132/290 (45.52%), Query Frame = 2
Query: 2918 VDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNI-SFFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNHWE--TFLKVNNLEFTVDLPGLSLCGGDPLNFIA----------EELEDMLSNNL-SNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSVQ---EYLKSKKEIIKRISNHKLENEIEILKSI--LNVASELNRDTLTRIYDLLITEKIVSHQAL 3727
V+ +E+ R +G ++LL +S Q +G + DD+ DIP IW + A+ + + + ++ F K +LP + FS LH ++ H+ VG ++ S L W +P + +F+ L+FT LS C P ++ ++LE +L +L S+E+IF+++E ++D+ + + +++ KA K+ SS + ++ + ++ + N E +++ L ++ L V E + L +D L E ++S A
Sbjct: 1414 VESTLERSQITRDHMGQLFHQLLQAGSLSKPQFFKGFSETLELADDMAIDIPHIWLYLAELVNPVLREGGISMRELFSEFSKPLLPVGRAGILFSEILHLLCKQMSHRKVGALWKDSGLSWADFLPETEDVNSFITEQKLDFT-----LSDC-SSPAAAVSNRMLSPEELNKQLEKLLLEDLASDEQIFDWVEANLDESEMSSPPFLRALMTAVCKAAVKLESSSYRVDTAIIQKRLPVLHKYLNSDTERQLQALYALQALIVKLEQPPNLLRMFFDCLYDEDVISEDAF 1697
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Cavefish
Match: eif4g3b (eukaryotic translation initiation factor 4 gamma 3 [Source:NCBI gene;Acc:103031287]) HSP 1 Score: 140.969 bits (354), Expect = 5.355e-33 Identity = 155/558 (27.78%), Postives = 266/558 (47.67%), Query Frame = 2
Query: 1028 ILLESKTPLSVKSFERMEFEEFDLQNKAPESSIRLDIEEDVATCSSEDEVHIDLKLSSSNYHLPVNKKESDETILKRKLIYDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISN--------QPRVISCQLPPESSSSQTNNERANNKKQLPNRQYSKYKNYNHKQTERKQI--NLEISDNQVFIRSKSGYLPPQLRDKQKLLDSLEELK-----RNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA---ITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVK-KAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKFN--LMTKKKNVSNSQNIARRN---IQKNVSIKNLS----CNSHKVDEKTVLGPVLG-NWATGS--GSKSKSHEAE 2593
+ +E ++ + E E E L+N PE + D E+V T + LK +S+N + K E + K Y+R +L+ + QKP +P ++ D I+ PRVIS A+ + +P + + N ++ ++I N+ ++D ++++ + P RD +D E LK R ++S LNK+TPQ F + +K++ L I+ +E + +I+ +F KA+ E ++SV Y C LA + + P F+ LL +CQ FE +D++++ E K++ L S + + E+ K KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K + L++K K Q N + KN + S + +D+K LGP NW GS G+ +K+ E++
Sbjct: 735 VCVERRSSTAAPVEENGEQEAEPLRN-GPEHTPEADSSEEVPTPA--------LKEASTNA---IQKPE----LKNGKRQYNRDFLLGFQFMPACVQKPEGLPPISDVVLDKINQNKLPLRQVDPRVIS-------RGPDFTPAFADFGRMIPGSRGAPLLNVGLRRPPPRKIIMNVSVNDEVQLKKAENAWKPGMKRDA--AIDDPENLKTQELFRKVRSILNKLTPQMFNQLMKQVTDLTIDTEERLKGVIDLVFEKAIDEPSFSVAYGNMCSCLATLKVPMADKPTSTVNFRKLLLNRCQKEFE---KDKMDDDVFEKKQRELEAASSATERERLQEELEEAKDKARRRSIGNIKFIGELFKLRMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLHNWVSRRADQ----GPKTIEQIHKEAKIEEQEEQRKVHQQLLSKDKRRPVLQREETWNTVPVPKNSRTIDPSKIPKISKPPMDDKIQLGPRAQLNWMKGSSGGAGAKASESD 1259 HSP 2 Score: 77.0258 bits (188), Expect = 1.551e-13 Identity = 67/290 (23.10%), Postives = 132/290 (45.52%), Query Frame = 2
Query: 2918 VDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNI-SFFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNHWE--TFLKVNNLEFTVDLPGLSLCGGDPLNFIA----------EELEDMLSNNL-SNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSVQ---EYLKSKKEIIKRISNHKLENEIEILKSI--LNVASELNRDTLTRIYDLLITEKIVSHQAL 3727
V+ +E+ R +G ++LL +S Q +G + DD+ DIP IW + A+ + + + ++ F K +LP + FS LH ++ H+ VG ++ S L W +P + +F+ L+FT LS C P ++ ++LE +L +L S+E+IF+++E ++D+ + + +++ KA K+ SS + ++ + ++ + N E +++ L ++ L V E + L +D L E ++S A
Sbjct: 1432 VESTLERSQITRDHMGQLFHQLLQAGSLSKPQFFKGFSETLELADDMAIDIPHIWLYLAELVNPVLREGGISMRELFSEFSKPLLPVGRAGILFSEILHLLCKQMSHRKVGALWKDSGLSWADFLPETEDVNSFITEQKLDFT-----LSDC-SSPAAAVSNRMLSPEELNKQLEKLLLEDLASDEQIFDWVEANLDESEMSSPPFLRALMTAVCKAAVKLESSSYRVDTAIIQKRLPVLHKYLNSDTERQLQALYALQALIVKLEQPPNLLRMFFDCLYDEDVISEDAF 1715
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Sea Lamprey
Match: eif4g2a (eukaryotic translation initiation factor 4, gamma 2a [Source:ZFIN;Acc:ZDB-GENE-030131-2173]) HSP 1 Score: 87.0409 bits (214), Expect = 2.084e-17 Identity = 77/244 (31.56%), Postives = 121/244 (49.59%), Query Frame = 2
Query: 1619 EELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLAITYTN--NPAFKGQLLKKCQDMFE----TPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYD------SDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLR 2311
E + R ++ LNK+TP+ F + E+ + I++K + +I IF KA++E YS Y+Q C +LA N P +G K F LQD+ EN + K L D++ L + +EQ AK K GNI+FIGEL +LL ++ CI LL ++ + LC+++ T G ++D K + M++Y + L + DL RIRF L+D +++R W R
Sbjct: 69 EAIFRKVRGILNKLTPEKFDKLCLELLNVGIDSKLILKGIILLIFEKALEEPKYSSLYSQLCQRLAEDAPNFDGPPLEGPGRHKHNSSFRRLLIAKLQDEFEN-----RTKKLEGFDNRDSPLSPEE-DEQRTIAKLKMLGNIKFIGELGKLDLLHESILHRCIKTLLEKKKRVALKDMAEDLECLCQIMRTVGPRLDHDKARSLMDQYFLRMQSLMANKDLPARIRFLLQDTVELRLNKWVPR 306
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Yeast
Match: TIF4631 (Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication [Source:SGD;Acc:S000003394]) HSP 1 Score: 83.1889 bits (204), Expect = 1.929e-16 Identity = 77/283 (27.21%), Postives = 129/283 (45.58%), Query Frame = 2
Query: 1595 KQKLLDSLEELKRNIKSNLNKITPQNFPE-----CVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA------ITYTNNPAFKGQ------LLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDS---DAMLALCKLLETCGKQID----------VKHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMR-ERNWTLRHVQQEKQINPKTL 2350
K +LLD +E++R +KS LNK+T + F + N E + +I +IF+KA E +S YAQ C K+ IT N G L+ +C F+ D+L NE + ++ S+ AK + G +RFIG L+ NLL GK++ C L++D DS + + ++ +LL T G+Q + ++ + ++ + + Q+ + RI+FKL DI ++R ++NW +K PKT+
Sbjct: 597 KTELLDK-DEVERKMKSLLNKLTLEMFDAISSEILAIANISVWETNGETLKAVIEQIFLKACDEPHWSSMYAQLCGKVVKELNPDITDETNEGKTGPKLVLHYLVARCHAEFDKGWTDKL----------PTNEDGTPLEPEMMSEEYYAAASAKRRGLGLVRFIGFLYRLNLLTGKMMFECFRRLMKDLTDSPSEETLESVVELLNTVGEQFETDSFRTGQATLEGSQLLDSLFGILDNIIQTAKISSRIKFKLIDIKELRHDKNWN----SDKKDNGPKTI 864
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Yeast
Match: TIF4632 (Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); associates with the poly(A)-binding protein Pab1p, also interacts with eIF4A (Tif1p); TIF4632 has a paralog, TIF4631, that arose from the whole genome duplication [Source:SGD;Acc:S000003017]) HSP 1 Score: 75.0998 bits (183), Expect = 5.266e-14 Identity = 94/358 (26.26%), Postives = 153/358 (42.74%), Query Frame = 2
Query: 1595 KQKLLDSLEELKRNIKSNLNKITPQNFPECVX-----XXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA------ITYTNNPAFKGQ------LLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRD---SYDSDAMLALCKLLETCGKQIDVKHKESMNKYISDISRLSQSVDLE----------------KRIRFKLKDIIDMRE-RNWTLRHVQQEKQINPKTLXX-XXXXXXXXXXXXTIKFNLMTKKKNVSNSQNIA--RRNIQ----------KNVSIKNLSCNSHKVDE 2518
K +L D EE++R +KS LNK+T + F + ++ E + +I +IF KA E +S YAQ C K+ I N G L+ +C + FE D+L E + ++ SD AK + G +RFIG L+ NLL GK++ C L++D + + ++ +LL T G+Q + +K+++ + L SV L+ RI+FKL D+ ++RE ++W +K PKT+++ + E + + N N SNS + RRN+Q K S +N N+ KV+E
Sbjct: 557 KTELFDK-EEVERKMKSLLNKLTLEMFDSISSEILDIANQSKWEDDGETLKIVIEQIFHKACDEPHWSSMYAQLCGKVVKDLDPNIKDKENEGKNGPKLVLHYLVARCHEEFEKGWADKL----------PAGEDGNPLEPEMMSDEYYIAAAAKRRGLGLVRFIGYLYCLNLLTGKMMFECFRRLMKDLNNDPSEETLESVIELLNTVGEQFE------HDKFVTPQATLEGSVLLDNLFMLLQHIIDGGTISNRIKFKLIDVKELREIKHWN----SAKKDAGPKTIQQIHQEEEQLRQKKNSQRSNSRFNNHNQSNSNRYSSNRRNMQNTQRDSFASTKTGSFRNNQRNARKVEE 893
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Nematostella
Match: EDO33172 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7ST03]) HSP 1 Score: 120.939 bits (302), Expect = 1.013e-27 Identity = 88/252 (34.92%), Postives = 137/252 (54.37%), Query Frame = 2
Query: 1622 ELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKL----AITYTNNPAFKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNET----DSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDVK-HKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTL 2350
E+ R + LNK+TPQ F + +++ L I+ E++ +I+ IF KA+ E +SV YA C L T F+ LL KCQ FE D EN +E ++++NE D K+K +++LEE+ K + GNIRFIGELF +L ++ C+++L++ S D +++ CKLL T GK +D + K M++Y S + ++ S R+RF L+D+ D+R NW R NPKT+
Sbjct: 1 EVMRKFRGILNKLTPQKFKKLTEQVMELEIDTSEKLEGIIDIIFEKAISEPGFSVAYANLCRCLLPIKVSTGNKELVFRTILLNKCQKEFEKEKTD--ENRIHEKIDQLVNEGLSEEDYKIK---KAELEEEEMFGKRRTLGNIRFIGELFKLKILTENIMHTCVMKLIK-SMDEESLECFCKLLSTIGKDLDHEVAKPRMDQYFSQMDKIIASKKTSSRVRFMLQDLRDLRLSNWVPRRDDN----NPKTI 242
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Nematostella
Match: EDO32341 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SVB3]) HSP 1 Score: 100.138 bits (248), Expect = 1.797e-23 Identity = 77/236 (32.63%), Postives = 127/236 (53.81%), Query Frame = 2
Query: 1631 RNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA---ITYTNNP-AFKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKM---KALYESDLEEQVKKAKDKYFGNIRFIGELF-LHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDVK-HKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLR 2311
R + LNKITPQNF + V+++ L I+ E++ +I+ +F KA++E Y V YA C+ L +++ N FK LL KCQ F+ + + N ++ ++NE S M K + S+ EE + + GNI FIGELF L +L ++ C+ +L+ + D +++ LC+LL T GK +D K M++Y +++ S +RF ++D+ D+R NW R
Sbjct: 4 RKFRGILNKITPQNFKKLVEQVMNLEIDTAEKLEGIIDIVFEKAIREPRYCVVYANLCHCLLPIKVSFENKELVFKTILLNKCQKKFDKTYEHKTHNNTDQ----LVNEGLSPMFSRKKKHPSEQEEYEMWERRRTLGNIIFIGELFILKKILTENIMHTCVWKLINRT-DDESLECLCELLSTVGKDLDHDIAKPRMDQYFRQFNKIIDSKKTSSCVRFMIQDLRDLRLSNWVPR 234
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Nematostella
Match: EDO31711 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SX49]) HSP 1 Score: 98.2117 bits (243), Expect = 1.310e-20 Identity = 86/272 (31.62%), Postives = 127/272 (46.69%), Query Frame = 2
Query: 1580 PQLRDKQKLLDSLEELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA--------ITYTNNPAFKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELL----RDSY--DSDAMLALCKLLETCGKQID-VKHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTL 2350
PQ D E + R ++ LNK+TP F + E+ + +E K + +I +F KA+ E AYS YAQ C +L+ T N F+ LLK+C++ F + K D K E EE+ K K GNIRFIGEL ++L ++ CI +LL R S S+ M LC L++T G +ID K K +++Y I ++ DL RIRF L+ I ++R W R V ++ + PK +
Sbjct: 53 PQYNRTNLYRDENELVFRRVRGILNKLTPDKFDKLFYELLHVGVETKYILKGVILLVFEKALDEPAYSSMYAQLCLRLSEEAPNFDDPGKTGNSTFRRLLLKQCEEEFNN-------------RSKASQAFDKKDDLTQEE--EEERGSTKRKMLGNIRFIGELAKLDMLHETILHKCIKQLLDKKKRASVADTSEDMECLCHLMKTVGSRIDGPKAKPLVDQYFERIYQIRSRSDLPSRIRFMLEGIEELRTNKWIPRKVARD--VGPKPI 307
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Medaka
Match: eif4g3a (eukaryotic translation initiation factor 4 gamma 3 [Source:NCBI gene;Acc:105354159]) HSP 1 Score: 136.346 bits (342), Expect = 1.187e-31 Identity = 131/475 (27.58%), Postives = 237/475 (49.89%), Query Frame = 2
Query: 1268 YDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISN---QPRVISCQLPPESSSSQTNNERANNKKQLPNRQYSKYKNYNHKQTERKQI--NLEISDNQVFIRSKSGYLPPQLRD---KQKLLDSLEELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA---ITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVK-KAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKFN--LMTK--KKNVSNSQNIARRNIQKNV--SIKN---------LSCNSHKVDEKTVLGP-VLGNWATGSGSKSKSHEAE 2593
Y+R +L+ + QKP +P ++ D ++ R + ++ S A+ +Q+ + + N ++ ++I N+ ++D+ ++++ + P R+ + +EL R ++S LNK+TPQ F + +K++ L I+ +E + +I+ +F KA+ E +SV Y C LA + T+ P F+ LL +CQ FE +D++++V E K+K L+ S + + E+ K KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K + L++K K+ + A+R+ N + KN + ++DEK LGP W GS +K+ ++E
Sbjct: 737 YNREFLLNFQFMPACTQKPEGLPPISDVVLDKMNQNKMPSRAVDSRV--ISRGPDFTPAFADFGRQMSGARGAALMNMGTRRPPPRKIIMNVTVNDDVQLKKAENAWKPGMKRETLPEDPEAQRTQELFRRVRSILNKLTPQKFNQLMKQVTDLTIDTEERLKGVIDLVFEKAIDEPGFSVAYGNMCRCLATLKVQMTDKPNSTVNFRKLLLNRCQKEFE---KDKVDDVVFERKQKELDSAASTTERERLQEELEEAKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLHNWVSRRADQ----GPKTIEQIHKEAKIEEQEEQRKVHQQLLSKDSKRRPDPREQRAQRDDTWNTVPTTKNSRTIDPNKIPKISKPQMDEKIQLGPRAQVTWVKGSSGGAKASDSE 1201 HSP 2 Score: 74.3294 bits (181), Expect = 9.955e-13 Identity = 73/321 (22.74%), Postives = 142/321 (44.24%), Query Frame = 2
Query: 2903 IIEKWVDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNI-SFFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNH--WETFLKVNNLEF-TVDLPGLSLCGGDPLNF------IAEELEDMLSNNL-SNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRK-------IPESSVQEYLKSKKEIIKRISNHKLENEIEILKSI--LNVASELNRDTLTRIYDLLITEKIVSHQAL------KDYCDNE----SLKSENNF 3772
++ VD +E+ R +G +L+ I+ Q +G + DD+ DIP IW + A+ L + D ++ F + K +LP + S LH ++ H+ V ++ +S + W ++P + F+ LEF D G C P ++++LE +L ++ S+E+IF+++E ++D+P +++ +++ KA K + + +Q L ++ + N E +++ L ++ L V+ E + L +D L E ++S A KD + E +LKS F
Sbjct: 1365 FVQVGVDSTLERSQITRDHMGQLFFQLVQQGILPKPQFYKGFTDTLEMADDMAIDIPHIWLYLAEMLCPVLKDGGFSMRELFSELSKPLLPVGRAGILISEILHILCKQMSHRTVSSLWRESGISWTDVLPQGEDVQAFISQQKLEFLQTDGSG---CEAAPSKRMLSPEELSQQLEKLLLEDMASDEQIFDWVEENLDEPQTSSPPFLRSLMTAVCKAAVKDENNCCRVDTAIIQRRLP----VLLKYLNSDTERQLQALYALQALIVSLEQPANLLRMFFDCLYDEDVISEDAFYKWESSKDPAEQEGKGVALKSVTAF 1678
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Medaka
Match: eif4g3a (eukaryotic translation initiation factor 4 gamma 3 [Source:NCBI gene;Acc:105354159]) HSP 1 Score: 135.961 bits (341), Expect = 1.445e-31 Identity = 131/475 (27.58%), Postives = 237/475 (49.89%), Query Frame = 2
Query: 1268 YDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISN---QPRVISCQLPPESSSSQTNNERANNKKQLPNRQYSKYKNYNHKQTERKQI--NLEISDNQVFIRSKSGYLPPQLRD---KQKLLDSLEELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA---ITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVK-KAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKFN--LMTK--KKNVSNSQNIARRNIQKNV--SIKN---------LSCNSHKVDEKTVLGP-VLGNWATGSGSKSKSHEAE 2593
Y+R +L+ + QKP +P ++ D ++ R + ++ S A+ +Q+ + + N ++ ++I N+ ++D+ ++++ + P R+ + +EL R ++S LNK+TPQ F + +K++ L I+ +E + +I+ +F KA+ E +SV Y C LA + T+ P F+ LL +CQ FE +D++++V E K+K L+ S + + E+ K KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K + L++K K+ + A+R+ N + KN + ++DEK LGP W GS +K+ ++E
Sbjct: 769 YNREFLLNFQFMPACTQKPEGLPPISDVVLDKMNQNKMPSRAVDSRV--ISRGPDFTPAFADFGRQMSGARGAALMNMGTRRPPPRKIIMNVTVNDDVQLKKAENAWKPGMKRETLPEDPEAQRTQELFRRVRSILNKLTPQKFNQLMKQVTDLTIDTEERLKGVIDLVFEKAIDEPGFSVAYGNMCRCLATLKVQMTDKPNSTVNFRKLLLNRCQKEFE---KDKVDDVVFERKQKELDSAASTTERERLQEELEEAKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLHNWVSRRADQ----GPKTIEQIHKEAKIEEQEEQRKVHQQLLSKDSKRRPDPREQRAQRDDTWNTVPTTKNSRTIDPNKIPKISKPQMDEKIQLGPRAQVTWVKGSSGGAKASDSE 1233 HSP 2 Score: 73.9442 bits (180), Expect = 1.154e-12 Identity = 73/321 (22.74%), Postives = 142/321 (44.24%), Query Frame = 2
Query: 2903 IIEKWVDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNI-SFFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNH--WETFLKVNNLEF-TVDLPGLSLCGGDPLNF------IAEELEDMLSNNL-SNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRK-------IPESSVQEYLKSKKEIIKRISNHKLENEIEILKSI--LNVASELNRDTLTRIYDLLITEKIVSHQAL------KDYCDNE----SLKSENNF 3772
++ VD +E+ R +G +L+ I+ Q +G + DD+ DIP IW + A+ L + D ++ F + K +LP + S LH ++ H+ V ++ +S + W ++P + F+ LEF D G C P ++++LE +L ++ S+E+IF+++E ++D+P +++ +++ KA K + + +Q L ++ + N E +++ L ++ L V+ E + L +D L E ++S A KD + E +LKS F
Sbjct: 1397 FVQVGVDSTLERSQITRDHMGQLFFQLVQQGILPKPQFYKGFTDTLEMADDMAIDIPHIWLYLAEMLCPVLKDGGFSMRELFSELSKPLLPVGRAGILISEILHILCKQMSHRTVSSLWRESGISWTDVLPQGEDVQAFISQQKLEFLQTDGSG---CEAAPSKRMLSPEELSQQLEKLLLEDMASDEQIFDWVEENLDEPQTSSPPFLRSLMTAVCKAAVKDENNCCRVDTAIIQRRLP----VLLKYLNSDTERQLQALYALQALIVSLEQPANLLRMFFDCLYDEDVISEDAFYKWESSKDPAEQEGKGVALKSVTAF 1710
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Medaka
Match: eif4g3a (eukaryotic translation initiation factor 4 gamma 3 [Source:NCBI gene;Acc:105354159]) HSP 1 Score: 135.961 bits (341), Expect = 1.473e-31 Identity = 131/475 (27.58%), Postives = 237/475 (49.89%), Query Frame = 2
Query: 1268 YDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISN---QPRVISCQLPPESSSSQTNNERANNKKQLPNRQYSKYKNYNHKQTERKQI--NLEISDNQVFIRSKSGYLPPQLRD---KQKLLDSLEELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLA---ITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVK-KAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKFN--LMTK--KKNVSNSQNIARRNIQKNV--SIKN---------LSCNSHKVDEKTVLGP-VLGNWATGSGSKSKSHEAE 2593
Y+R +L+ + QKP +P ++ D ++ R + ++ S A+ +Q+ + + N ++ ++I N+ ++D+ ++++ + P R+ + +EL R ++S LNK+TPQ F + +K++ L I+ +E + +I+ +F KA+ E +SV Y C LA + T+ P F+ LL +CQ FE +D++++V E K+K L+ S + + E+ K KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D+ID+R NW R Q PKT+E+ EA E K + L++K K+ + A+R+ N + KN + ++DEK LGP W GS +K+ ++E
Sbjct: 776 YNREFLLNFQFMPACTQKPEGLPPISDVVLDKMNQNKMPSRAVDSRV--ISRGPDFTPAFADFGRQMSGARGAALMNMGTRRPPPRKIIMNVTVNDDVQLKKAENAWKPGMKRETLPEDPEAQRTQELFRRVRSILNKLTPQKFNQLMKQVTDLTIDTEERLKGVIDLVFEKAIDEPGFSVAYGNMCRCLATLKVQMTDKPNSTVNFRKLLLNRCQKEFE---KDKVDDVVFERKQKELDSAASTTERERLQEELEEAKDKARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLHNWVSRRADQ----GPKTIEQIHKEAKIEEQEEQRKVHQQLLSKDSKRRPDPREQRAQRDDTWNTVPTTKNSRTIDPNKIPKISKPQMDEKIQLGPRAQVTWVKGSSGGAKASDSE 1240 HSP 2 Score: 73.9442 bits (180), Expect = 1.184e-12 Identity = 73/321 (22.74%), Postives = 142/321 (44.24%), Query Frame = 2
Query: 2903 IIEKWVDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNI-SFFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNH--WETFLKVNNLEF-TVDLPGLSLCGGDPLNF------IAEELEDMLSNNL-SNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRK-------IPESSVQEYLKSKKEIIKRISNHKLENEIEILKSI--LNVASELNRDTLTRIYDLLITEKIVSHQAL------KDYCDNE----SLKSENNF 3772
++ VD +E+ R +G +L+ I+ Q +G + DD+ DIP IW + A+ L + D ++ F + K +LP + S LH ++ H+ V ++ +S + W ++P + F+ LEF D G C P ++++LE +L ++ S+E+IF+++E ++D+P +++ +++ KA K + + +Q L ++ + N E +++ L ++ L V+ E + L +D L E ++S A KD + E +LKS F
Sbjct: 1404 FVQVGVDSTLERSQITRDHMGQLFFQLVQQGILPKPQFYKGFTDTLEMADDMAIDIPHIWLYLAEMLCPVLKDGGFSMRELFSELSKPLLPVGRAGILISEILHILCKQMSHRTVSSLWRESGISWTDVLPQGEDVQAFISQQKLEFLQTDGSG---CEAAPSKRMLSPEELSQQLEKLLLEDMASDEQIFDWVEENLDEPQTSSPPFLRSLMTAVCKAAVKDENNCCRVDTAIIQRRLP----VLLKYLNSDTERQLQALYALQALIVSLEQPANLLRMFFDCLYDEDVISEDAFYKWESSKDPAEQEGKGVALKSVTAF 1717
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Medaka
Match: eif4g1a (eukaryotic translation initiation factor 4 gamma 1 [Source:NCBI gene;Acc:101158558]) HSP 1 Score: 133.265 bits (334), Expect = 1.081e-30 Identity = 88/252 (34.92%), Postives = 144/252 (57.14%), Query Frame = 2
Query: 1622 ELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFC---YKLAITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNET-DSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTL 2350
EL + ++S LNK+TPQ F E +K++ L I+ +E + I+ IF KA+ E +SV YA C L + + P F+ LL +CQ FE +DQ ++ E K+K L+ + D + + +LEE KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D++D+R NW R + PKT+
Sbjct: 831 ELFKRLRSILNKLTPQKFQELMKQVSELAIDTEERLKGAIDLIFEKAISEPNFSVAYANMCRCLMGLKVPTPDKPGNFVNFRKLLLNRCQKEFE---KDQDDDEIFERKQKELDASKDDEERERLRVELEESKDKARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLSTIGKDLDFEKAKPRMDQYFTQMDKIIKEKKTSSRIRFMLQDVLDLRRNNWVPRR----GDLGPKTI 1074 HSP 2 Score: 62.3882 bits (150), Expect = 4.450e-9 Identity = 43/194 (22.16%), Postives = 95/194 (48.97%), Query Frame = 2
Query: 2918 VDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNIS-FFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNHWE--TFLKVNNLEFTVDLPGLS--------LCGGDPLNFIAEELEDMLSNNLSNEKIFEFLETHVD 3463
V+ +E+ R +G L+ L+ + +QQ +G + + + +D+ DIP IW + A+ + +++ + F I K ++P + + L + + VG ++ ++ L W +I+P + TF+ +EFTVD S L G + I+++L+ +L + +N++I +++E +++
Sbjct: 1396 VESTLERSTIAREHMGLLLHNLVKAGTLPTQQYFKGLEEILEAAEDMAIDIPHIWLYLAELITPMLHEGGIPMGQLFREISKPLVPLGKAGVLLAQILQLLCKGMTPQKVGALWKEAGLSWNEILPKDEDVNTFVTDQKVEFTVDEEMKSKDVGRKNVLSGEE----ISKQLDRLLQDKANNQRIRDWIEANLE 1585
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Medaka
Match: eif4g1a (eukaryotic translation initiation factor 4 gamma 1 [Source:NCBI gene;Acc:101158558]) HSP 1 Score: 133.265 bits (334), Expect = 1.084e-30 Identity = 88/252 (34.92%), Postives = 144/252 (57.14%), Query Frame = 2
Query: 1622 ELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFC---YKLAITYTNNPA----FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNET-DSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDV-KHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTL 2350
EL + ++S LNK+TPQ F E +K++ L I+ +E + I+ IF KA+ E +SV YA C L + + P F+ LL +CQ FE +DQ ++ E K+K L+ + D + + +LEE KA+ + GNI+FIGELF +L ++ +C+V+LL++ +D +++ LC+LL T GK +D K K M++Y + + ++ + RIRF L+D++D+R NW R + PKT+
Sbjct: 787 ELFKRLRSILNKLTPQKFQELMKQVSELAIDTEERLKGAIDLIFEKAISEPNFSVAYANMCRCLMGLKVPTPDKPGNFVNFRKLLLNRCQKEFE---KDQDDDEIFERKQKELDASKDDEERERLRVELEESKDKARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKN-HDEESLECLCRLLSTIGKDLDFEKAKPRMDQYFTQMDKIIKEKKTSSRIRFMLQDVLDLRRNNWVPRR----GDLGPKTI 1030 HSP 2 Score: 62.3882 bits (150), Expect = 4.283e-9 Identity = 43/194 (22.16%), Postives = 95/194 (48.97%), Query Frame = 2
Query: 2918 VDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNIS-FFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKS-LKWEKIVPNHWE--TFLKVNNLEFTVDLPGLS--------LCGGDPLNFIAEELEDMLSNNLSNEKIFEFLETHVD 3463
V+ +E+ R +G L+ L+ + +QQ +G + + + +D+ DIP IW + A+ + +++ + F I K ++P + + L + + VG ++ ++ L W +I+P + TF+ +EFTVD S L G + I+++L+ +L + +N++I +++E +++
Sbjct: 1352 VESTLERSTIAREHMGLLLHNLVKAGTLPTQQYFKGLEEILEAAEDMAIDIPHIWLYLAELITPMLHEGGIPMGQLFREISKPLVPLGKAGVLLAQILQLLCKGMTPQKVGALWKEAGLSWNEILPKDEDVNTFVTDQKVEFTVDEEMKSKDVGRKNVLSGEE----ISKQLDRLLQDKANNQRIRDWIEANLE 1541
BLAST of Eukaryotic initiation factor-4G vs. Planmine SMEST
Match: SMESG000045275.1 (SMESG000045275.1) HSP 1 Score: 1924.06 bits (4983), Expect = 0.000e+0 Identity = 976/976 (100.00%), Postives = 976/976 (100.00%), Query Frame = 2
Query: 896 MCTTLMNTEEDSSYEKENVEYTTKESNIICDKEETEQSKKEDDDILLESKTPLSVKSFERMEFEEFDLQNKAPESSIRLDIEEDVATCSSEDEVHIDLKLSSSNYHLPVNKKESDETILKRKLIYDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQPRVISCQLPPESSSSQTNNERANNKKQLPNRQYSKYKNYNHKQTERKQINLEISDNQVFIRSKSGYLPPQLRDKQKLLDSLEELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLAITYTNNPAFKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDVKHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKFNLMTKKKNVSNSQNIARRNIQKNVSIKNLSCNSHKVDEKTVLGPVLGNWATGSGSKSKSHEAEGWTPVSQKPKGGAMRYTSNSRSREQSPQVILANKYRNLESSAEASGADSRSSTPPMIRPITKAFRIIEQEVTDEEMAEIIFNDLLSTGNFDIKKWLTLRKVQPIIEKWVDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKSLKWEKIVPNHWETFLKVNNLEFTVDLPGLSLCGGDPLNFIAEELEDMLSNNLSNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSVQEYLKSKKEIIKRISNHKLENEIEILKSILNVASELNRDTLTRIYDLLITEKIVSHQALKDYCDNESLKSENNFKLTNSLLSDILYLNEKS 3823
MCTTLMNTEEDSSYEKENVEYTTKESNIICDKEETEQSKKEDDDILLESKTPLSVKSFERMEFEEFDLQNKAPESSIRLDIEEDVATCSSEDEVHIDLKLSSSNYHLPVNKKESDETILKRKLIYDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQPRVISCQLPPESSSSQTNNERANNKKQLPNRQYSKYKNYNHKQTERKQINLEISDNQVFIRSKSGYLPPQLRDKQKLLDSLEELKRNIKSNLNKITPQNFPECVKEIEILNIENKEEVSCLINEIFIKAVKEEAYSVTYAQFCYKLAITYTNNPAFKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDVKHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLEEQKAEAAAEANAATIKFNLMTKKKNVSNSQNIARRNIQKNVSIKNLSCNSHKVDEKTVLGPVLGNWATGSGSKSKSHEAEGWTPVSQKPKGGAMRYTSNSRSREQSPQVILANKYRNLESSAEASGADSRSSTPPMIRPITKAFRIIEQEVTDEEMAEIIFNDLLSTGNFDIKKWLTLRKVQPIIEKWVDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKSLKWEKIVPNHWETFLKVNNLEFTVDLPGLSLCGGDPLNFIAEELEDMLSNNLSNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSVQEYLKSKKEIIKRISNHKLENEIEILKSILNVASELNRDTLTRIYDLLITEKIVSHQALKDYCDNESLKSENNFKLTNSLLSDILYLNEKS
Sbjct: 1 MCTTLMNTEEDSSYEKENVEYTTKESNIICDKEETEQSKKEDDDILLESKTPLSVKSFERMEFEEFDLQNKAPESSIRLDIEEDVATCSSEDEVHIDLKLSSSNYHLPVNKKESDETILKRKLIYDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQPRVISCQLPPESSSSQTNNERANNKKQLPNRQYSKYKNYNHKQTERKQINLEISDNQVFIRSKSGYLPPQLRDKQKLLDSLEELKRNIKSNLNKITPQNFPECVKEIEILNIENKEEVSCLINEIFIKAVKEEAYSVTYAQFCYKLAITYTNNPAFKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDVKHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLEEQKAEAAAEANAATIKFNLMTKKKNVSNSQNIARRNIQKNVSIKNLSCNSHKVDEKTVLGPVLGNWATGSGSKSKSHEAEGWTPVSQKPKGGAMRYTSNSRSREQSPQVILANKYRNLESSAEASGADSRSSTPPMIRPITKAFRIIEQEVTDEEMAEIIFNDLLSTGNFDIKKWLTLRKVQPIIEKWVDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKSLKWEKIVPNHWETFLKVNNLEFTVDLPGLSLCGGDPLNFIAEELEDMLSNNLSNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSVQEYLKSKKEIIKRISNHKLENEIEILKSILNVASELNRDTLTRIYDLLITEKIVSHQALKDYCDNESLKSENNFKLTNSLLSDILYLNEKS 976
BLAST of Eukaryotic initiation factor-4G vs. Planmine SMEST
Match: SMESG000045275.1 (SMESG000045275.1) HSP 1 Score: 1912.5 bits (4953), Expect = 0.000e+0 Identity = 971/971 (100.00%), Postives = 971/971 (100.00%), Query Frame = 2
Query: 911 MNTEEDSSYEKENVEYTTKESNIICDKEETEQSKKEDDDILLESKTPLSVKSFERMEFEEFDLQNKAPESSIRLDIEEDVATCSSEDEVHIDLKLSSSNYHLPVNKKESDETILKRKLIYDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQPRVISCQLPPESSSSQTNNERANNKKQLPNRQYSKYKNYNHKQTERKQINLEISDNQVFIRSKSGYLPPQLRDKQKLLDSLEELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLAITYTNNPAFKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDVKHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKFNLMTKKKNVSNSQNIARRNIQKNVSIKNLSCNSHKVDEKTVLGPVLGNWATGSGSKSKSHEAEGWTPVSQKPKGGAMRYTSNSRSREQSPQVILANKYRNLESSAEASGADSRSSTPPMIRPITKAFRIIEQEVTDEEMAEIIFNDLLSTGNFDIKKWLTLRKVQPIIEKWVDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKSLKWEKIVPNHWETFLKVNNLEFTVDLPGLSLCGGDPLNFIAEELEDMLSNNLSNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSVQEYLKSKKEIIKRISNHKLENEIEILKSILNVASELNRDTLTRIYDLLITEKIVSHQALKDYCDNESLKSENNFKLTNSLLSDILYLNEKS 3823
MNTEEDSSYEKENVEYTTKESNIICDKEETEQSKKEDDDILLESKTPLSVKSFERMEFEEFDLQNKAPESSIRLDIEEDVATCSSEDEVHIDLKLSSSNYHLPVNKKESDETILKRKLIYDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQPRVISCQLPPESSSSQTNNERANNKKQLPNRQYSKYKNYNHKQTERKQINLEISDNQVFIRSKSGYLPPQLRDKQKLLDSLEELKRNIKSNLNKITPQNFPECVKEIEILNIENKEEVSCLINEIFIKAVKEEAYSVTYAQFCYKLAITYTNNPAFKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDVKHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLEEQKAEAAAEANAATIKFNLMTKKKNVSNSQNIARRNIQKNVSIKNLSCNSHKVDEKTVLGPVLGNWATGSGSKSKSHEAEGWTPVSQKPKGGAMRYTSNSRSREQSPQVILANKYRNLESSAEASGADSRSSTPPMIRPITKAFRIIEQEVTDEEMAEIIFNDLLSTGNFDIKKWLTLRKVQPIIEKWVDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKSLKWEKIVPNHWETFLKVNNLEFTVDLPGLSLCGGDPLNFIAEELEDMLSNNLSNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSVQEYLKSKKEIIKRISNHKLENEIEILKSILNVASELNRDTLTRIYDLLITEKIVSHQALKDYCDNESLKSENNFKLTNSLLSDILYLNEKS
Sbjct: 1 MNTEEDSSYEKENVEYTTKESNIICDKEETEQSKKEDDDILLESKTPLSVKSFERMEFEEFDLQNKAPESSIRLDIEEDVATCSSEDEVHIDLKLSSSNYHLPVNKKESDETILKRKLIYDRHYLIKMKDNLKSRQKPINMPATTEFSEDIISNQPRVISCQLPPESSSSQTNNERANNKKQLPNRQYSKYKNYNHKQTERKQINLEISDNQVFIRSKSGYLPPQLRDKQKLLDSLEELKRNIKSNLNKITPQNFPECVKEIEILNIENKEEVSCLINEIFIKAVKEEAYSVTYAQFCYKLAITYTNNPAFKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDVKHKESMNKYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLEEQKAEAAAEANAATIKFNLMTKKKNVSNSQNIARRNIQKNVSIKNLSCNSHKVDEKTVLGPVLGNWATGSGSKSKSHEAEGWTPVSQKPKGGAMRYTSNSRSREQSPQVILANKYRNLESSAEASGADSRSSTPPMIRPITKAFRIIEQEVTDEEMAEIIFNDLLSTGNFDIKKWLTLRKVQPIIEKWVDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKSLKWEKIVPNHWETFLKVNNLEFTVDLPGLSLCGGDPLNFIAEELEDMLSNNLSNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKIPESSVQEYLKSKKEIIKRISNHKLENEIEILKSILNVASELNRDTLTRIYDLLITEKIVSHQALKDYCDNESLKSENNFKLTNSLLSDILYLNEKS 971
BLAST of Eukaryotic initiation factor-4G vs. Planmine SMEST
Match: SMESG000030556.1 (SMESG000030556.1) HSP 1 Score: 384.03 bits (985), Expect = 2.395e-113 Identity = 284/794 (35.77%), Postives = 426/794 (53.65%), Query Frame = 2
Query: 1556 RSKSGYLPPQLRDKQK--LLDSLEELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXX-------VSCLINEIFIKAVKEEAYSVTYAQFCYKLAITYTNNPA---FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDVKHKESMNKYISDISRLSQSVD-LEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXX-----TIKFNLMTKKKNVSNSQNIARRNIQKNVSIKNLSCNSHKVDEKTVLGPVLGNWATGSGSKSKSHEAEGWTPVSQKPKGGAMRYTSNSRSREQ--SPQVIL--------ANKYRNLESSAEASGADSRSSTPPMIRPITKAFRIIEQEVTDEEMAEIIFNDLLSTGNFDIKKWLTLRKVQPIIEKWVDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKSLKWEKIVPNHWETFLKV--NNLEFTVDLPGLSLCGGDPLNFIAEELEDMLSNNL------SNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKI-PESSVQEYLKSKKEIIKRISNHKLENEIEILKSILNVASEL-NRDTLTRIYDLLITEKIVSHQALKDYCDNESLKS---EN---NFKLTNSLLSDIL 3805
R +GYLPP LR K++ L +LE +RN+K LNKI PQNF C+ EI+ + ++ + CLI EIF KAVKEEAYSVTYA C+ +A +N + FK LL +C+ MFETPLQDQL V+N W++KV E D K++ LY SD+EEQ+KKA+DKYFGNIRFIGEL++H+LL K+I C+ L+ D DAML+LCKLLETCGK+ D KH + M+ YI ++ + ++ D LE RIRFK+ DII+M ER W LR VQ +K + PKTLEE K + + + + + TIK N T K++ N + + +N SI +++ S E L P G W + K + W V K + + +Y+S ++S + SP I +KY L + + + +S P +R ++ E + + E++ N LL T ++ W KV +I +W+D +MEK R +GD L L+ + ++EG F+ I+DLI DIPKIW F A+ LERFI D+ S + F++ +F+S LPN + C L A+ R+G ++ + ++ N + FL + + +FT L L + + + + N L NE IFE LE+ + E ++ + + + ES V ++L K+ + +RI + K E + L +++N A+ L RD LT + L T KI+S A + + +N LK EN N++LT + + IL
Sbjct: 283 RIVNGYLPPHLRLKEECSLNKNLENCRRNLKCILNKIAPQNFSLCINEIKAIIKSIDQQNVKIDDCIDCLIEEIFDKAVKEEAYSVTYANLCFNIAKLDDDNESKWNFKASLLNRCKQMFETPLQDQLRMVKNHWEKKVEEEEDEKLRNLYRSDIEEQIKKARDKYFGNIRFIGELYVHDLLKPKIIHCCVNTLIPKPKDEDAMLSLCKLLETCGKKFDEKHSDKMSDYIKSMTEMLETPDLLETRIRFKILDIIEMSERKWLLREVQSQKMVRPKTLEEVKKDVSDDKSISASKFPTIKSNNRTSTKHIPNQSELKFKQQSRNQSI-DVNSLSLGTSESLELKPSYGFWNSN-----KEKDQSEWKNVDNKKRTKS-KYSSTNKSSQNRFSPNKITDEPTNKAHLSKYAILSTQDDVKENNITNSESP-VRSVSPCESKSEDDAVMMDGEELLDN-LLETHTLNMTAWGNQLKVSELINQWLDKIMEKKQRDRHLVGDQLANLVACNKLQKDALIEGLNQFIDLIEDLITDIPKIWTFLAEILERFIQDEKSYVEFYKFVFESRLPNEHINKCVLLILEYANERVGSSVM----------KFLIDNQLDDFLNLIKKDKDFTSMPETLILNTDNTNSSTTTQFTNSFKNELISSLPNDNENIFELLESKFTIEQMQTDEFVNSLAYCLFSFICDLKSESDVIDFLIEKQCLFRRIIDSKSAFETQFLLALVNFANRLETRDLLTTLVQNL-TGKIISTDACQHFINNSELKDLDLENDNENYQLTVNAIRSIL 1056
BLAST of Eukaryotic initiation factor-4G vs. Planmine SMEST
Match: SMESG000030556.1 (SMESG000030556.1) HSP 1 Score: 383.645 bits (984), Expect = 3.869e-113 Identity = 284/794 (35.77%), Postives = 426/794 (53.65%), Query Frame = 2
Query: 1556 RSKSGYLPPQLRDKQK--LLDSLEELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXX-------VSCLINEIFIKAVKEEAYSVTYAQFCYKLAITYTNNPA---FKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDVKHKESMNKYISDISRLSQSVD-LEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXX-----TIKFNLMTKKKNVSNSQNIARRNIQKNVSIKNLSCNSHKVDEKTVLGPVLGNWATGSGSKSKSHEAEGWTPVSQKPKGGAMRYTSNSRSREQ--SPQVIL--------ANKYRNLESSAEASGADSRSSTPPMIRPITKAFRIIEQEVTDEEMAEIIFNDLLSTGNFDIKKWLTLRKVQPIIEKWVDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFENIFKSILPNNDVALCFSTALHEASSRLGHKIVGQVFHKSLKWEKIVPNHWETFLKV--NNLEFTVDLPGLSLCGGDPLNFIAEELEDMLSNNL------SNEKIFEFLETHVDKPTWKKSECTKTITSSILKALRKI-PESSVQEYLKSKKEIIKRISNHKLENEIEILKSILNVASEL-NRDTLTRIYDLLITEKIVSHQALKDYCDNESLKS---EN---NFKLTNSLLSDIL 3805
R +GYLPP LR K++ L +LE +RN+K LNKI PQNF C+ EI+ + ++ + CLI EIF KAVKEEAYSVTYA C+ +A +N + FK LL +C+ MFETPLQDQL V+N W++KV E D K++ LY SD+EEQ+KKA+DKYFGNIRFIGEL++H+LL K+I C+ L+ D DAML+LCKLLETCGK+ D KH + M+ YI ++ + ++ D LE RIRFK+ DII+M ER W LR VQ +K + PKTLEE K + + + + + TIK N T K++ N + + +N SI +++ S E L P G W + K + W V K + + +Y+S ++S + SP I +KY L + + + +S P +R ++ E + + E++ N LL T ++ W KV +I +W+D +MEK R +GD L L+ + ++EG F+ I+DLI DIPKIW F A+ LERFI D+ S + F++ +F+S LPN + C L A+ R+G ++ + ++ N + FL + + +FT L L + + + + N L NE IFE LE+ + E ++ + + + ES V ++L K+ + +RI + K E + L +++N A+ L RD LT + L T KI+S A + + +N LK EN N++LT + + IL
Sbjct: 292 RIVNGYLPPHLRLKEECSLNKNLENCRRNLKCILNKIAPQNFSLCINEIKAIIKSIDQQNVKIDDCIDCLIEEIFDKAVKEEAYSVTYANLCFNIAKLDDDNESKWNFKASLLNRCKQMFETPLQDQLRMVKNHWEKKVEEEEDEKLRNLYRSDIEEQIKKARDKYFGNIRFIGELYVHDLLKPKIIHCCVNTLIPKPKDEDAMLSLCKLLETCGKKFDEKHSDKMSDYIKSMTEMLETPDLLETRIRFKILDIIEMSERKWLLREVQSQKMVRPKTLEEVKKDVSDDKSISASKFPTIKSNNRTSTKHIPNQSELKFKQQSRNQSI-DVNSLSLGTSESLELKPSYGFWNSN-----KEKDQSEWKNVDNKKRTKS-KYSSTNKSSQNRFSPNKITDEPTNKAHLSKYAILSTQDDVKENNITNSESP-VRSVSPCESKSEDDAVMMDGEELLDN-LLETHTLNMTAWGNQLKVSELINQWLDKIMEKKQRDRHLVGDQLANLVACNKLQKDALIEGLNQFIDLIEDLITDIPKIWTFLAEILERFIQDEKSYVEFYKFVFESRLPNEHINKCVLLILEYANERVGSSVM----------KFLIDNQLDDFLNLIKKDKDFTSMPETLILNTDNTNSSTTTQFTNSFKNELISSLPNDNENIFELLESKFTIEQMQTDEFVNSLAYCLFSFICDLKSESDVIDFLIEKQCLFRRIIDSKSAFETQFLLALVNFANRLETRDLLTTLVQNL-TGKIISTDACQHFINNSELKDLDLENDNENYQLTVNAIRSIL 1065
BLAST of Eukaryotic initiation factor-4G vs. Planmine SMEST
Match: SMESG000045255.1 (SMESG000045255.1) HSP 1 Score: 363.614 bits (932), Expect = 2.065e-103 Identity = 230/584 (39.38%), Postives = 336/584 (57.53%), Query Frame = 2
Query: 1550 FIRSKSGYLPPQLRDKQKLLDSLEELKRNIKSNLNKITPQNFPECVXXXXXXXXXXXXXVSCLINEIFIKAVKEEAYSVTYAQFCYKLAITYTNNPAFKGQLLKKCQDMFETPLQDQLENVRNEWKEKVLNETDSKMKALYESDLEEQVKKAKDKYFGNIRFIGELFLHNLLAGKVILNCIVELLRDSYDSDAMLALCKLLETCGKQIDVKHKESMN------KYISDISRLSQSVDLEKRIRFKLKDIIDMRERNWTLRHVQQEKQINPKTLXXXXXXXXXXXXXXTIKFNLMTKKKNVSNSQNIARRNIQKN--VSIKNLSCNSHKVDEKTVLGPVL-GNWATGSGSKSKSHEAEGWTPVSQKPKGGAMRYTSNSRSREQSPQVIL---ANKYRNLESSAEASGADSRSSTPPMIRPITKAFRIIEQEVTDEEMAEIIFNDLLSTGNFDIKKWLTLRKVQPIIEKWVDDMMEKDVSCRRQIGDSLNRLLIDKIISSQQIVEGTQVFVSSIDDLICDIPKIWEFFAQFLERFIYDKNSNISFFENIFKSILPN-NDVALCFSTALHEASSRLGHKIVGQVFHK 3262
IR+++GYLPPQLR + D L+ LKRNIKS LNK+TP+NF C+ ++ L I+ +E + CLI+EIF KA++E +YS YA+FC +++ N +FK LLK CQ MF+ PL QLE V+ W+ K NE D +MK LYESD+EEQVKKAKDKYFGNIRF ELFL N L+ K+I+ C LL S D++++ A C+L+E CGKQ++ KE N + ++ LS++VD+E+R+RFKLKD++DMRERNW LR +Q+ + PKTL+E K + E + N +N+ N+ R + K L N + E TVLGP L W +G +SKS+ + G++ + P + Y P V++ NKY L E S P ++ E E+ +++ N+L + + KW L ++EKW M E+ V CR ++GD + L+ +++ Q+++G V + ++DD+I D+PK WEF AQFLERFI ++SNISFF+++FK + + V CF +SSR+ IV +F K
Sbjct: 1011 LIRTENGYLPPQLRKADE--DKLQLLKRNIKSILNKMTPENFEVCLNDLVNLEIDEEENMRCLIDEIFSKAIQEVSYSSIYARFCSEMSNARVGNYSFKALLLKSCQTMFDKPLHLQLEEVKTFWEAKAANEKDERMKRLYESDIEEQVKKAKDKYFGNIRFFSELFLSNQLSWKIIIACAERLLMFSKDNNSLEAACRLMEICGKQLEKLEKERTNGDKGFSRVMNQFVVLSKNVDIEQRLRFKLKDVVDMRERNWELRDIQKAMKSVPKTLDEIKEDKQKEDLSKIKTNYNNNNNNNNTNNNNMRNRQPYSRPVMDTKKLLVNPQNLSENTVLGPSLRTQW--NAGCQSKSNNSNGFSTI---PSRKQVSYDDII----DKPTVVVIKTQNKYAKLCEDFEV----ELPSVP---------VKVNNDEALTTELFDLLMNNLAT-----VDKWKELFNPVEVVEKWFALMFERKVECRMRVGDYFHDLIEGGLLTFDQLIQGASVTMETVDDIIDDVPKFWEFLAQFLERFIRSEHSNISFFKDLFKDVEGQESGVEKCFKFTQQFSSSRIDSDIVEALFGK 1565 The following BLAST results are available for this feature:
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Eukaryotic initiation factor-4G vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Eukaryotic initiation factor-4G vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 2
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 3
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Eukaryotic initiation factor-4G vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30025106 ID=SMED30025106|Name=Eukaryotic initiation factor-4G|organism=Schmidtea mediterranea sexual|type=transcript|length=4301bpback to top protein sequence of SMED30025106-orf-1 >SMED30025106-orf-1 ID=SMED30025106-orf-1|Name=SMED30025106-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1229bp MSQKFELRKYNYNFQCFFNLKLIPIGIWTRTKYVFYCNVCYKANSFIMIIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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