CHHC U11-48K-type domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30025085 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of CHHC U11-48K-type domain-containing protein vs. Ensembl Fly
Match: CG14321 (gene:FBgn0038540 transcript:FBtr0083511) HSP 1 Score: 46.9802 bits (110), Expect = 1.693e-6 Identity = 20/31 (64.52%), Postives = 24/31 (77.42%), Query Frame = 2 Query: 158 QCMNNKCYQEIYQMNELEEGEIDRRYYSFRG 250 QC++ CYQ+IY NELEEGEID R SF+G Sbjct: 85 QCISPSCYQDIYAFNELEEGEIDARLNSFKG 115
BLAST of CHHC U11-48K-type domain-containing protein vs. TrEMBL
Match: W4YCH2 (CHHC U11-48K-type domain-containing protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1) HSP 1 Score: 60.4622 bits (145), Expect = 9.249e-7 Identity = 42/145 (28.97%), Postives = 70/145 (48.28%), Query Frame = 3 Query: 318 IDNILSQINWSKESIENTEIKEICKYNCCHSISLSSKSNHESKCLMLNNGWNKKEYESIPKIQLKPVIGDGLGGVHKLELNH-LEERLQSRINQSDDNTDLLSSKGRNRREKELELLAEVRDIKRKRQSYR---VYKEIKSQTEV 740 +D IL +I WS E +++ E C N H + SS H +C +G+ ++E E ++ + VH +EL+ + + SR +DD + + E LAE+RD +R+RQSYR V+ +S+T+V Sbjct: 33 LDGILKEIGWSFEDLQSKEALVTCPINPQHRVVESSLQCHIDRCKWARDGYTREEQEEAASLEANRFFYENKKDVHAVELDADCQREITSRFVDNDDKKETTQTHA--------EALAEMRDYRRRRQSYRAKNVHITKRSKTDV 169
BLAST of CHHC U11-48K-type domain-containing protein vs. Planmine SMEST
Match: SMESG000009746.1 (SMESG000009746.1) HSP 1 Score: 353.599 bits (906), Expect = 4.172e-124 Identity = 177/180 (98.33%), Postives = 179/180 (99.44%), Query Frame = 3 Query: 201 MNLKKVKSIDVIILFEDITDSDKNFVSNCQVFLSDSESLIDNILSQINWSKESIENTEIKEICKYNCCHSISLSSKSNHESKCLMLNNGWNKKEYESIPKIQLKPVIGDGLGGVHKLELNHLEERLQSRINQSDDNTDLLSSKGRNRREKELELLAEVRDIKRKRQSYRVYKEIKSQTEV 740 MNLKKVKSIDVIILFEDITDSDKNFVSNCQVFLSDSESLI+NILSQINWSKESIENTEIKEICKYNCCHSISLSSKSNHESKCLMLNNGWNKKEYESIPKI LKPVIGDGLGGVHKLELNHLEERLQSRINQSDDNTDLLSSKGRNRREKELELLAEVRDIKRKRQSYRVYKEIKSQTE+ Sbjct: 1 MNLKKVKSIDVIILFEDITDSDKNFVSNCQVFLSDSESLIENILSQINWSKESIENTEIKEICKYNCCHSISLSSKSNHESKCLMLNNGWNKKEYESIPKIPLKPVIGDGLGGVHKLELNHLEERLQSRINQSDDNTDLLSSKGRNRREKELELLAEVRDIKRKRQSYRVYKEIKSQTEI 180 The following BLAST results are available for this feature:
BLAST of CHHC U11-48K-type domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of CHHC U11-48K-type domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of CHHC U11-48K-type domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 1
BLAST of CHHC U11-48K-type domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of CHHC U11-48K-type domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of CHHC U11-48K-type domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of CHHC U11-48K-type domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of CHHC U11-48K-type domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 1
BLAST of CHHC U11-48K-type domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of CHHC U11-48K-type domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of CHHC U11-48K-type domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of CHHC U11-48K-type domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of CHHC U11-48K-type domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of CHHC U11-48K-type domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30025085 ID=SMED30025085|Name=CHHC U11-48K-type domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=976bpback to top protein sequence of SMED30025085-orf-1 >SMED30025085-orf-1 ID=SMED30025085-orf-1|Name=SMED30025085-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=184bp MNLKKVKSIDVIILFEDITDSDKNFVSNCQVFLSDSESLIDNILSQINWSback to top Annotated Terms
The following terms have been associated with this transcript:
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