SMED30025035

Overview
NameSMED30025035
Smed IDSMED30025035
Length (bp)525
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30025035

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 36

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30025035h1SMcG0002130 Contig1264GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30025035h1SMcG0002130 Contig1264GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30025035h1SMcG0002129 Contig1264GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30025035h1SMcG0002129 Contig1264GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30025035h1SMcG0017311 Contig1264GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30025035h1SMcG0017311 Contig1264GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30025035h1SMcG0002130 Contig1264GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30025035h1SMcG0002130 Contig1264GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30025035h1SMcG0002129 Contig1264GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30025035h1SMcG0002129 Contig1264GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30025035h1SMcG0017311 Contig1264GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30025035h1SMcG0017311 Contig1264GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
head regionSMED30025035h1SMcG0002130 SmedASXL_012923SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30025035h1SMcG0002131 SmedASXL_012923SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30025035h1SMcG0002130 SmedASXL_012923SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30025035h1SMcG0002131 SmedASXL_012923SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30025035h1SMcG0002130 Contig35429newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025035h1SMcG0002130 Contig35429uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
parenchymaSMED30025035h1SMcG0002130 dd_Smed_v4_14898_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025035h1SMcG0002131 Contig35429newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025035h1SMcG0002131 Contig35429uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025035h1SMcG0002130 Contig35429newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025035h1SMcG0002130 Contig35429uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
parenchymaSMED30025035h1SMcG0002130 dd_Smed_v4_14898_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025035h1SMcG0002131 Contig35429newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30025035h1SMcG0002131 Contig35429uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30025035h1SMcG0002131 OX_Smed_1.0.02541ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
head regionSMED30025035h1SMcG0002130 OX_Smed_1.0.02541ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
head regionSMED30025035h1SMcG0010683 OX_Smed_1.0.02541ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
head regionSMED30025035h1SMcG0010684 OX_Smed_1.0.02541ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
head regionSMED30025035h1SMcG0002131 OX_Smed_1.0.02541ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
head regionSMED30025035h1SMcG0002130 OX_Smed_1.0.02541ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
head regionSMED30025035h1SMcG0010683 OX_Smed_1.0.02541ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
head regionSMED30025035h1SMcG0010684 OX_Smed_1.0.02541ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30025035h1SMcG0002130 dd_Smed_v4_14949_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30025035h1SMcG0002130 dd_Smed_v4_14949_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30025035 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.008627:12192..12716 +2508
v31.053761:300..823 -2500
Homology
[Close]
BLAST of SMED30025035 vs. Ensembl Zebrafish
Match: CR762475.1 (pep chromosome:GRCz11:4:55494751:55498830:1 gene:ENSDARG00000098243.2 transcript:ENSDART00000172605.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR762475.1)

HSP 1 Score: 52.373 bits (124), Expect = 5.029e-8
Identity = 35/96 (36.46%), Postives = 50/96 (52.08%), Query Frame = -1 
Query:  220 LIVDSTPDVIHINQLSFILRYVPPDGYVKERFFSFIDIQGHDTTYF-HIFIDTLKKLDIHITKCNDQSYD-ATNMSEPYIGLQDRIKAKIPIADYI 501
            +++D T DV H  Q+SF++RYV  D  VKERF     ++        ++ I  L+   + +     Q YD A NMS  Y GLQ RI+A    A Y+
Sbjct:  407 ILLDETTDVSHKEQVSFVVRYV-RDMEVKERFLQVCTLESTTGKELENVVISLLQDNGLDLKNIRGQGYDGAANMSGMYKGLQSRIRAHNEKALYV 501
[Close]
BLAST of SMED30025035 vs. Ensembl Xenopus
Match: ENSXETT00000043220.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:7:36411169:36451375:1 gene:ENSXETG00000018966.1 transcript:ENSXETT00000043220.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 94.3597 bits (233), Expect = 2.209e-22
Identity = 54/102 (52.94%), Postives = 63/102 (61.76%), Query Frame = -1 
Query:  220 KCKYCVLIVDSTPDVIHINQLSFILRYVPPDGYVKERFFSFIDIQGHDT-TYFHIFIDTLKKLDIHITKCNDQSYD-ATNMSEPYIGLQDRIKAKIPIADYI 519
            + KY  + VDSTPDV H +QL+FI+ YV  DG  KERF  FI   GH     F   +DTLK+  I I  C  QSYD A+NMS PY GLQ RIK   P+A YI
Sbjct:  244 QAKYYSISVDSTPDVSHTDQLAFIICYVKNDGIPKERFIKFIPNVGHKAENLFSAVMDTLKEYHIDILDCRGQSYDNASNMSGPYSGLQARIKQVNPLAVYI 345
[Close]
BLAST of SMED30025035 vs. TrEMBL
Match: A0A5N4ARD5 (Uncharacterized protein OS=Photinus pyralis OX=7054 GN=PPYR_07658 PE=4 SV=1)

HSP 1 Score: 104.76 bits (260), Expect = 8.794e-23
Identity = 54/99 (54.55%), Postives = 65/99 (65.66%), Query Frame = -1 
Query:  220 YCVLIVDSTPDVIHINQLSFILRYVPPDGYVKERFFSFIDIQGHDTTYF-HIFIDTLKKLDIHITKCNDQSYD-ATNMSEPYIGLQDRIKAKIPIADYI 510
            Y  LI+DSTPD+ H++QLS +LRYV PDG +KERFF FIDI  H +    H+  +TL    I ITKC  QSYD A NM+  Y GLQ RIK     A+YI
Sbjct:  658 YFSLIIDSTPDISHVDQLSIVLRYVLPDGQIKERFFGFIDIYSHHSENLEHVVTETLSNWGIDITKCRGQSYDNAANMAGKYSGLQARIKQHSANAEYI 756
[Close]
BLAST of SMED30025035 vs. TrEMBL
Match: A0A4Y2KXS9 (DUF4371 domain-containing protein (Fragment) OS=Araneus ventricosus OX=182803 GN=AVEN_221066_1 PE=4 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 3.563e-21
Identity = 47/99 (47.47%), Postives = 64/99 (64.65%), Query Frame = -1 
Query:  220 YCVLIVDSTPDVIHINQLSFILRYVPPDGYVKERFFSFIDIQGHDTTYF-HIFIDTLKKLDIHITKCNDQSY-DATNMSEPYIGLQDRIKAKIPIADYI 510
            Y  +IVDSTPDV HI+QL+FILR+V   G +KE FF FI I+ HD+ Y  ++ +  L+   I +  C  ++Y DA+NMS  YIGLQ R+K     A Y+
Sbjct:    2 YYSIIVDSTPDVTHIDQLTFILRFVDEKGDIKENFFGFIPIESHDSAYLQNVVLGNLRNYSIELKNCRGKTYDDASNMSGRYIGLQARLKEHCKTATYV 100
[Close]
BLAST of SMED30025035 vs. TrEMBL
Match: A0A4Y2HNS7 (Zinc finger MYM-type protein 1 OS=Araneus ventricosus OX=182803 GN=ZMYM1_63 PE=4 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 8.352e-21
Identity = 48/99 (48.48%), Postives = 65/99 (65.66%), Query Frame = -1 
Query:  220 YCVLIVDSTPDVIHINQLSFILRYVPPDGYVKERFFSFIDIQGHDTTYF-HIFIDTLKKLDIHITKCNDQSYD-ATNMSEPYIGLQDRIKAKIPIADYI 510
            Y  +IVDSTPDV HI+QL+FILR+V   G +KERFF FI I+ HD+ Y  ++ ++ L+   I +  C  Q+YD A+NMS  Y GLQ R+K     A Y+
Sbjct:  434 YYSIIVDSTPDVTHIDQLTFILRFVDEKGDIKERFFGFIPIESHDSAYLENVVLENLRNYSIELKNCRGQTYDNASNMSGRYTGLQARLKEHCKTATYV 532
[Close]
BLAST of SMED30025035 vs. TrEMBL
Match: A0A4Y2TIA1 (DUF4371 domain-containing protein OS=Araneus ventricosus OX=182803 GN=AVEN_147296_1 PE=4 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 9.932e-21
Identity = 47/99 (47.47%), Postives = 64/99 (64.65%), Query Frame = -1 
Query:  220 YCVLIVDSTPDVIHINQLSFILRYVPPDGYVKERFFSFIDIQGHDTTYF-HIFIDTLKKLDIHITKCNDQSYD-ATNMSEPYIGLQDRIKAKIPIADYI 510
            Y  +IVDSTPDV HI+QL+FILR+V   G +KERFF FI I+  D+ Y  ++ ++ L+   I +  C  Q+YD A+NMS  Y GLQ R+K     A Y+
Sbjct:   18 YYSIIVDSTPDVTHIDQLTFILRFVDEKGDIKERFFGFIPIESQDSAYLENVVLENLRNYSIELKNCRGQTYDNASNMSGRYTGLQARLKEHCKTATYV 116
[Close]
BLAST of SMED30025035 vs. TrEMBL
Match: A0A2S2Q4N5 (Zinc finger MYM-type protein 1 OS=Sipha flava OX=143950 GN=ZMYM1_27 PE=4 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 1.077e-20
Identity = 54/103 (52.43%), Postives = 68/103 (66.02%), Query Frame = -1 
Query:  220 KCKYCVLIVDSTPDVIHINQLSFILRYVPPDGYVKERFFSFIDIQGH--DTTYFHIFIDTLKKLDIHITKCNDQSYD-ATNMSEPYIGLQDRIKAKIPIADYI 519
            K KY  LIVDSTPD+ H++QLS+ILRYV  +G V ERF  F +I  H  ++   HI +  LK+L+I I  C  QSYD A+NMS  Y GLQ RIK   P A+Y+
Sbjct:  129 KVKYFGLIVDSTPDIAHVDQLSYILRYVTEEGEVLERFIKFQNIYSHTAESLTTHI-VSLLKELNISINNCIGQSYDNASNMSGKYSGLQARIKQLSPKAEYV 230
[Close]
BLAST of SMED30025035 vs. Ensembl Cavefish
Match: ENSAMXT00000042715.1 (pep primary_assembly:Astyanax_mexicanus-2.0:16:11855083:11857132:-1 gene:ENSAMXG00000035113.1 transcript:ENSAMXT00000042715.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 82.4185 bits (202), Expect = 1.721e-18
Identity = 41/100 (41.00%), Postives = 63/100 (63.00%), Query Frame = -1 
Query:  220 KYCVLIVDSTPDVIHINQLSFILRYVPPDGYVKERFFSFIDIQGHD-TTYFHIFIDTLKKLDIHITKCNDQSYD-ATNMSEPYIGLQDRIKAKIPIADYI 513
            KY  +IVDSTPD+ H++QL+FI R+V  DG+V ERF +F  I+ H   +     +  ++ L + ++ C  QSYD A+NMS  Y G+Q  +K + P+  Y+
Sbjct:  217 KYFSVIVDSTPDLAHVDQLTFIFRFVNNDGHVVERFLAFEPIENHSGDSLAECVVAMVENLGLDLSNCRGQSYDNASNMSGKYNGIQAHLKQRNPLICYV 316
[Close]
BLAST of SMED30025035 vs. Ensembl Cavefish
Match: ENSAMXT00000035531.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000029.1:6285841:6288423:1 gene:ENSAMXG00000041958.1 transcript:ENSAMXT00000035531.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 73.9442 bits (180), Expect = 1.660e-15
Identity = 38/100 (38.00%), Postives = 60/100 (60.00%), Query Frame = -1 
Query:  220 KYCVLIVDSTPDVIHINQLSFILRYVPPDGYVKERFFSFIDIQGHD-TTYFHIFIDTLKKLDIHITKCNDQSYD-ATNMSEPYIGLQDRIKAKIPIADYI 513
            KY  +IVDSTPD+ H++Q +FI R+V  D +V ERF +F  I+ H   +     +  ++ L + ++ C  QSYD A+NMS  Y G+Q  +K +  +  Y+
Sbjct:  376 KYFSVIVDSTPDLAHVDQPTFIFRFVNNDAHVVERFLAFEPIENHSGDSLAECVVAMVENLGLDLSNCRGQSYDNASNMSGKYNGVQAHLKQRNSLICYV 475
[Close]
BLAST of SMED30025035 vs. Ensembl Cavefish
Match: ENSAMXT00000044961.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000032.1:419978:423709:1 gene:ENSAMXG00000036481.1 transcript:ENSAMXT00000044961.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 63.5438 bits (153), Expect = 7.397e-12
Identity = 38/96 (39.58%), Postives = 55/96 (57.29%), Query Frame = -1 
Query:  250 KCKYCVLIVDSTPDVIHINQLSFILRYV--PPDGYVKERFFSFIDIQGHDTT---YFHIFIDTLKKLDIHITKCNDQSYD-ATNMSEPYIGLQDRI 519
            + KY  +I+D TPD+ H  QLS +LR V   P   + E FF FID++  DT+      + +D L+K ++ I+ C  QSYD  +NM     G+Q RI
Sbjct:  303 RAKYYSVIMDCTPDISHKEQLSVVLRLVNCDPSVSIAEHFFGFIDVE--DTSGKGLTEVLLDHLQKHNLSISDCRGQSYDNGSNMMGQKQGVQARI 396
[Close]
BLAST of SMED30025035 vs. Ensembl Cavefish
Match: ENSAMXT00000025204.2 (pep primary_assembly:Astyanax_mexicanus-2.0:12:7364240:7371214:-1 gene:ENSAMXG00000024490.2 transcript:ENSAMXT00000025204.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.3806 bits (137), Expect = 1.004e-9
Identity = 35/90 (38.89%), Postives = 52/90 (57.78%), Query Frame = -1 
Query:  250 LIVDSTPDVIHINQLSFILRYV--PPDGYVKERFFSFIDIQGHDTT---YFHIFIDTLKKLDIHITKCNDQSYD-ATNMSEPYIGLQDRI 501
            +I+D TPD+ H  QLS +LR V   P   + E FF FID++  DT+      + +D L+K ++ I+ C  QSYD  +NM     G++ RI
Sbjct:  255 VIMDCTPDISHKEQLSVVLRLVNCDPSVSIAEHFFGFIDVE--DTSGKGLTEVLLDHLQKHNLSISDCRGQSYDNGSNMMGQKQGVRARI 342
[Close]
BLAST of SMED30025035 vs. Ensembl Cavefish
Match: ENSAMXT00000036272.1 (pep primary_assembly:Astyanax_mexicanus-2.0:20:11935414:11938921:1 gene:ENSAMXG00000041670.1 transcript:ENSAMXT00000036272.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.1434 bits (126), Expect = 2.745e-8
Identity = 29/104 (27.88%), Postives = 55/104 (52.88%), Query Frame = -1 
Query:  220 KCKYCVLIVDSTPDVIHINQLSFILRYVP--PDGYVKERFFSFID-IQGHDTTYFHIFIDTLKKLDIHITKCNDQSYD-ATNMSEPYIGLQDRIKAKIPIADYI 519
            + KY  +I+D TPD+ H  Q+S ++R V   P+ +++E F  +++ ++       ++ ++ L+ L I    C  Q+YD   NM     G+Q R+    P A ++
Sbjct:  329 QAKYYAIILDCTPDISHKEQMSLVVRIVELVPEPHIREYFLGYMNVVETTGLNLSNVVLEKLRALGIPFEDCRGQAYDNGANMKGKRQGVQARLLQLNPRAAFV 432
[Close]
BLAST of SMED30025035 vs. Ensembl Medaka
Match: ENSORLT00000028133.1 (zinc finger MYM-type protein 1-like [Source:NCBI gene;Acc:111948955])

HSP 1 Score: 50.8322 bits (120), Expect = 1.758e-7
Identity = 35/106 (33.02%), Postives = 55/106 (51.89%), Query Frame = -1 
Query:  220 KCKYCVLIVDSTPDVIHINQLSFILRYVP--PDGYVKERFFSFIDIQGHDTTYFHI---FIDTLKKLDIHITKCNDQSYD-ATNMSEPYIGLQDRIKAKIPIADYI 519
            K KY  +I+D T D+ H  QLS I+R V       ++E F  F++ +  ++T  H+    ++ L++L I    C  QSYD   NM     G+Q R+  K P A ++
Sbjct:  361 KAKYFSIILDCTLDISHTEQLSVIIRVVSLMEKPQIREHFMGFLEAE--ESTGQHLASMILNRLEELGISFEDCRGQSYDNGANMKGKNKGVQARLLEKNPRALFL 464
[Close]
BLAST of SMED30025035 vs. Ensembl Medaka
Match: ENSORLT00000042294.1 (zinc finger MYM-type protein 1-like [Source:NCBI gene;Acc:110016639])

HSP 1 Score: 50.8322 bits (120), Expect = 1.758e-7
Identity = 35/106 (33.02%), Postives = 55/106 (51.89%), Query Frame = -1 
Query:  220 KCKYCVLIVDSTPDVIHINQLSFILRYVP--PDGYVKERFFSFIDIQGHDTTYFHI---FIDTLKKLDIHITKCNDQSYD-ATNMSEPYIGLQDRIKAKIPIADYI 519
            K KY  +I+D T D+ H  QLS I+R V       ++E F  F++ +  ++T  H+    ++ L++L I    C  QSYD   NM     G+Q R+  K P A ++
Sbjct:  361 KAKYFSIILDCTLDISHTEQLSVIIRVVSLMEKPQIREHFMGFLEAE--ESTGQHLASMILNRLEELGISFEDCRGQSYDNGANMKGKNKGVQARLLEKNPRALFL 464
[Close]
BLAST of SMED30025035 vs. Ensembl Medaka
Match: ENSORLT00000045044.1 (zinc finger MYM-type protein 1-like [Source:NCBI gene;Acc:111948193])

HSP 1 Score: 50.8322 bits (120), Expect = 1.758e-7
Identity = 35/106 (33.02%), Postives = 55/106 (51.89%), Query Frame = -1 
Query:  220 KCKYCVLIVDSTPDVIHINQLSFILRYVP--PDGYVKERFFSFIDIQGHDTTYFHI---FIDTLKKLDIHITKCNDQSYD-ATNMSEPYIGLQDRIKAKIPIADYI 519
            K KY  +I+D T D+ H  QLS I+R V       ++E F  F++ +  ++T  H+    ++ L++L I    C  QSYD   NM     G+Q R+  K P A ++
Sbjct:  361 KAKYFSIILDCTLDISHTEQLSVIIRVVSLMEKPQIREHFMGFLEAE--ESTGQHLASMILNRLEELGISFEDCRGQSYDNGANMKGKNKGVQARLLEKNPRALFL 464
[Close]
BLAST of SMED30025035 vs. Planmine SMEST
Match: SMESG000018951.1 (SMESG000018951.1)

HSP 1 Score: 75.0998 bits (183), Expect = 3.836e-16
Identity = 39/71 (54.93%), Postives = 48/71 (67.61%), Query Frame = -1 
Query:  301 YCVLIVDSTPDVIHINQLSFILRYVPPDGYVKERFFSFIDIQGHDTTY-FHIFIDTLKKLDIHITKCNDQS 510
            Y  LIV+ST  V HI+QL+F++ YV PDGYVKE+FFSFIDIQ HD  Y F + IDT KK    +     +S
Sbjct:  290 YFALIVESTLAVSHIDQLTFVISYVLPDGYVKEKFFSFIDIQCHDPAYLFQVMIDTFKKWGFTLQNTEAKS 360
[Close]
BLAST of SMED30025035 vs. Planmine SMEST
Match: SMESG000060573.1 (SMESG000060573.1)

HSP 1 Score: 64.6994 bits (156), Expect = 1.702e-12
Identity = 38/98 (38.78%), Postives = 56/98 (57.14%), Query Frame = -1 
Query:  229 VDSTPDVIHINQLSFILRYVPPDGY-----VKERFFSFIDIQ---GHDTTYFHIFIDTLKKLDIHITKCNDQSYD-ATNMSEPYIGLQDRIKAKIPIA 495
             D+TPD  HI Q +FILRYV  + +     +KERF +F++     G D    ++ ++TL+K +I I+KC  Q YD  +NMS  Y G +  I    P+A
Sbjct:   77 ADATPDSAHIEQTTFILRYVSVNSHSDEYEIKERFLAFVNCNKKTGEDIA--NLILETLEKYNIPISKCRAQGYDNGSNMSGSYKGAEGCILQINPLA 172
[Close]
BLAST of SMED30025035 vs. Planmine SMEST
Match: SMESG000033201.1 (SMESG000033201.1)

HSP 1 Score: 62.7734 bits (151), Expect = 2.157e-12
Identity = 36/91 (39.56%), Postives = 51/91 (56.04%), Query Frame = -1 
Query:  250 CKYCVLIVDSTPDVIHINQLSFILRYVPPDGYVKERFFSFIDIQGHDTTY-FHIFIDTLKKLDIHITKCNDQSYD-ATNMSEPYIGLQDRI 516
             KY    + S  D+ H +QL+F + YV  DG   ERF  F  I  H + + F   ++T + L+I I  C  Q+YD A+NMS+ Y G+Q RI
Sbjct:   16 AKYFSFSIYSNSDISHTDQLTFTVHYVKIDGQSVERFLKF-QISSHTSLHQFDTILETFRALEIDINDCRGQTYDNASNMSKQYSGVQARI 105
[Close]
BLAST of SMED30025035 vs. Planmine SMEST
Match: SMESG000003766.1 (SMESG000003766.1)

HSP 1 Score: 62.003 bits (149), Expect = 9.860e-12
Identity = 38/100 (38.00%), Postives = 54/100 (54.00%), Query Frame = -1 
Query:  223 VDSTPDVIHINQLSFILRYVPP-DGYVKERFFSFIDIQGHDTTYFH--IFIDTLKKLDIHITKCNDQSYD-ATNMSEPYIG-----LQDRIKAKIPIADY 495
            VDSTP + H +QLS I+ YV P DG   ERF +F++++ H        +F     +L+I   KC  QSYD A NM+  Y G     L ++ K  +P  +Y
Sbjct:   33 VDSTPVISHTDQLSLIIIYVSPEDGLPTERFLAFLELKDHSVESIADLVFNSITTELEIDFRKCRGQSYDNAANMAGRYNGKILIHLSNKQKTNLPNTEY 132
[Close]
BLAST of SMED30025035 vs. Planmine SMEST
Match: SMESG000049700.1 (SMESG000049700.1)

HSP 1 Score: 56.6102 bits (135), Expect = 9.926e-10
Identity = 41/120 (34.17%), Postives = 57/120 (47.50%), Query Frame = -1 
Query:  181 KCKYCVLIVDSTPDVIHINQLSFILRYVPPDGY-----VKERFFSFIDIQGHD-TTYFHIFIDTLKKLDIHITKCNDQSYD-ATNMSEPYIGLQDRIKAKIPIADYIR*TILKQNLKKIH 519
            + KY     DSTPD  H   LS + RYV  D       +KE F  FI++   D  T  ++ +DTL+  ++ +  C  Q YD A  MS    GLQ RI  + P A ++       NL  +H
Sbjct:  253 RNKYYGSFFDSTPDQAHREHLSRVKRYVDVDFSNKTVTIKESFLRFIELHAKDAATLEYVIVDTLEADNLRLDDCRSQCYDNAALMSGHISGLQQRICNRNPRAIFVNCNNYSLNLAGMH 372
The following BLAST results are available for this feature:
BLAST of SMED30025035 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025035 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025035 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025035 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CR762475.15.029e-836.46pep chromosome:GRCz11:4:55494751:55498830:1 gene:E... [more]
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BLAST of SMED30025035 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSXETT00000043220.12.209e-2252.94pep primary_assembly:Xenopus_tropicalis_v9.1:7:364... [more]
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BLAST of SMED30025035 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025035 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025035 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5N4ARD58.794e-2354.55Uncharacterized protein OS=Photinus pyralis OX=705... [more]
A0A4Y2KXS93.563e-2147.47DUF4371 domain-containing protein (Fragment) OS=Ar... [more]
A0A4Y2HNS78.352e-2148.48Zinc finger MYM-type protein 1 OS=Araneus ventrico... [more]
A0A4Y2TIA19.932e-2147.47DUF4371 domain-containing protein OS=Araneus ventr... [more]
A0A2S2Q4N51.077e-2052.43Zinc finger MYM-type protein 1 OS=Sipha flava OX=1... [more]
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BLAST of SMED30025035 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000042715.11.721e-1841.00pep primary_assembly:Astyanax_mexicanus-2.0:16:118... [more]
ENSAMXT00000035531.11.660e-1538.00pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000044961.17.397e-1239.58pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000025204.21.004e-938.89pep primary_assembly:Astyanax_mexicanus-2.0:12:736... [more]
ENSAMXT00000036272.12.745e-827.88pep primary_assembly:Astyanax_mexicanus-2.0:20:119... [more]
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BLAST of SMED30025035 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025035 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025035 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30025035 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSORLT00000028133.11.758e-733.02zinc finger MYM-type protein 1-like [Source:NCBI g... [more]
ENSORLT00000042294.11.758e-733.02zinc finger MYM-type protein 1-like [Source:NCBI g... [more]
ENSORLT00000045044.11.758e-733.02zinc finger MYM-type protein 1-like [Source:NCBI g... [more]
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BLAST of SMED30025035 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000018951.13.836e-1654.93SMESG000018951.1[more]
SMESG000060573.11.702e-1238.78SMESG000060573.1[more]
SMESG000033201.12.157e-1239.56SMESG000033201.1[more]
SMESG000003766.19.860e-1238.00SMESG000003766.1[more]
SMESG000049700.19.926e-1034.17SMESG000049700.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30025035 ID=SMED30025035|Name=SMED30025035|organism=Schmidtea mediterranea sexual|type=transcript|length=525bp
AAAGTTAAATAATTCAATTATCGTCGACACAAAAAGCCTATGATCAGGCA
GAGTTTTACTTATTGCATTTATTCTTTGTATCAAACAAAGCAGCTTTTAT
TTCGTAGTACTTTTTCTTTAAATCCAGAACAGCAACTGGTCTCCTAGAAC
ACCTGATATCAGAAACTTTTTTCACAAACAGTGAATTTTCTTAAGATTTT
GTTTCAAAATGGTTCACCGAATGTAATCTGCAATCGGGATTTTAGCTTTG
ATGCGATCTTGCAATCCAATGTATGGCTCTGACATATTGGTAGCGTCGTA
GCTTTGGTCATTACATTTTGTAATATGTATGTCCAATTTCTTAAGAGTAT
CAATAAATATATGAAAATATGTAGTATCATGACCTTGGATATCAATAAAA
GAAAAGAATCTTTCTTTTACGTATCCGTCCGGAGGAACATATCTAAGGAT
AAAACTGAGCTGATTAATATGAATTACATCGGGTGTTGAATCAACAATCA
ATACACAATACTTGCACTTCATAAA
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protein sequence of SMED30025035-orf-1

>SMED30025035-orf-1 ID=SMED30025035-orf-1|Name=SMED30025035-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=104bp
FMKCKYCVLIVDSTPDVIHINQLSFILRYVPPDGYVKERFFSFIDIQGHD
TTYFHIFIDTLKKLDIHITKCNDQSYDATNMSEPYIGLQDRIKAKIPIAD
YIR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR025398DUF4371