SMED30024938

Overview
NameSMED30024938
Smed IDSMED30024938
Length (bp)1926
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30024938 (SMED30024938) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30024938 (SMED30024938) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30024938 (SMED30024938) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30024938 (SMED30024938) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30024938

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pigment cellSMED30024938SMESG000014710.1 SmedASXL_019210SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult RNA-sequencing evidence
gutSMED30024938SMESG000014710.1 dd_Smed_v4_2336_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30024938SMESG000014710.1 dd_Smed_v4_2336_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30024938SMESG000014710.1 dd_Smed_v4_2336_0_1dd_Smed_v4PMID:30962434
Cote et al., 2019		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30024938 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002992:7213..12389 -9428
Homology
[Close]
BLAST of SMED30024938 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2])

HSP 1 Score: 56.9954 bits (136), Expect = 4.651e-8
Identity = 51/191 (26.70%), Postives = 84/191 (43.98%), Query Frame = 2 
Query:  380 QYANNRILEIYYINPNSTKERRNMLNHLKPTQLMYRNNINLLVPPQLELKMNSQVRNYITVEPNSSLTVSCQALDGQPKIRPEIV-KSDMKIEFDIFSHDSNTH---KITIRTSML------DHNRTVTCKAGSVMKSVRI---------FIYHAPTKIKLQPEVKKVLGNNTGIICLATNGYPTPRITWY 895
            Q A    L+I ++N +   E    + H +   +  ++ +N++VPPQL    N Q  + I V+ N+ L ++C   + +P+  PE++   D K+        S  H     T+ TS++      DH + +TC+A      +RI         F    PT   L+       G+N  I C  T G P P + WY
Sbjct:   82 QNAEELHLKITHVNLDDDGEYECQMLHPEEGPIRAKSFLNIIVPPQLVYFSNYQPNSIIAVKENTPLNITCVVPNVKPE--PEVLWYMDGKVMSRDVKQASTPHLNKTFTVYTSLVVQSDRNDHGKVITCEAFQKETDIRITTNTTLDVLFPPSDPTVEILRNPSALRSGDNVTIACSVTGGNPPPDVFWY 270
[Close]
BLAST of SMED30024938 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2])

HSP 1 Score: 56.6102 bits (135), Expect = 5.254e-8
Identity = 51/191 (26.70%), Postives = 84/191 (43.98%), Query Frame = 2 
Query:  380 QYANNRILEIYYINPNSTKERRNMLNHLKPTQLMYRNNINLLVPPQLELKMNSQVRNYITVEPNSSLTVSCQALDGQPKIRPEIV-KSDMKIEFDIFSHDSNTH---KITIRTSML------DHNRTVTCKAGSVMKSVRI---------FIYHAPTKIKLQPEVKKVLGNNTGIICLATNGYPTPRITWY 895
            Q A    L+I ++N +   E    + H +   +  ++ +N++VPPQL    N Q  + I V+ N+ L ++C   + +P+  PE++   D K+        S  H     T+ TS++      DH + +TC+A      +RI         F    PT   L+       G+N  I C  T G P P + WY
Sbjct:   82 QNAEELHLKITHVNLDDDGEYECQMLHPEEGPIRAKSFLNIIVPPQLVYFSNYQPNSIIAVKENTPLNITCVVPNVKPE--PEVLWYMDGKVMSRDVKQASTPHLNKTFTVYTSLVVQSDRNDHGKVITCEAFQKETDIRITTNTTLDVLFPPSDPTVEILRNPSALRSGDNVTIACSVTGGNPPPDVFWY 270
[Close]
BLAST of SMED30024938 vs. Ensembl Zebrafish
Match: hmcn1 (hemicentin 1 [Source:ZFIN;Acc:ZDB-GENE-041014-322])

HSP 1 Score: 54.299 bits (129), Expect = 5.066e-7
Identity = 53/201 (26.37%), Postives = 88/201 (43.78%), Query Frame = 2 
Query:  491 NINLLVPPQLELKMNSQVRNYITVEPNSSLTVSCQALDGQPKIRPEIVKSDMKIEFDIFSHDSNTHKI--------TIRTSMLDHNRTVTCK---AGSVMKSVRIFIYHAPTKIKLQPE-VKKVLGNNTGIICLATNGYPTPRITWYFIHGPSDASLYYQIAPYTQRLYIKENVPIGSSWKFICSATNSFFERSKTFNKTV 1057
            ++ +L+PP +    + ++ + +TV  N ++ + CQ +DG P  +   VK    +  D      NTH++         +   + D  R V      AG+  KS  + ++ +PT + ++ E V  VL N   + C AT G P P + W+    P  AS    I P  + L I +   +    K+ C A N   E  K    TV
Sbjct: 2846 DVRVLLPPSIR-GADGELPDEVTVLVNKTMLLECQ-VDGSPTPKISWVKDSQPLTQD------NTHRLLSNGRTLQILNAQVTDTGRYVCVAQNLAGTAEKSFNLHVHVSPTIVGIREENVTVVLNNFVSLNCEAT-GLPPPTLRWFKDRRPVQASTNALIMPGGRTLQILK-AKMSDGGKYSCVAVNPAGEAQKLIYLTV 3036

HSP 2 Score: 50.447 bits (119), Expect = 8.419e-6
Identity = 54/202 (26.73%), Postives = 87/202 (43.07%), Query Frame = 2 
Query:  491 NINLLVPPQLELKMNSQVRNYITVEPNSSLTVSCQALDGQPKI-----RPEIVKSDMKIEFDIFSHDSNTHKITIRTSMLDHNRTVTCKA----GSVMKSVRIFIYHAPTKI--KLQPEVKKVLGNNTGIICLATNGYPTPRITWYF--IHGPSDASLYYQIAPYTQRLYIKENVPIGSSWKFICSATNSFFERSKTFNKTV 1057
            ++N+ VPP+L+     +V   I+    + +T +C A    P          ++++   +E  I S  S   K+ I  S L  + T TC A    G   K+  + I+  P  I  +L  E+  +L ++  ++C A  G PTP I W    +     A    +I+P    L +   V    S K+ C ATN   E  + FN  V
Sbjct: 3128 HLNIHVPPRLDGPAEERVVETIS----NPVTFACDATGIPPPSLTWLKNGRVIENSESLEMHILSGGS---KLQISRSQLSDSGTYTCVASNVEGKAQKNYHLVIHVPPNIIGSELPSEMSVLLNDSVQLVCRA-EGTPTPEIQWLKDGMTISRTAQKNIKISPDGSTLTVTA-VHTSDSGKYTCVATNQAGEEDRIFNLNV 3320
[Close]
BLAST of SMED30024938 vs. Ensembl Zebrafish
Match: hmcn1 (hemicentin 1 [Source:ZFIN;Acc:ZDB-GENE-041014-322])

HSP 1 Score: 54.299 bits (129), Expect = 5.066e-7
Identity = 53/201 (26.37%), Postives = 88/201 (43.78%), Query Frame = 2 
Query:  491 NINLLVPPQLELKMNSQVRNYITVEPNSSLTVSCQALDGQPKIRPEIVKSDMKIEFDIFSHDSNTHKI--------TIRTSMLDHNRTVTCK---AGSVMKSVRIFIYHAPTKIKLQPE-VKKVLGNNTGIICLATNGYPTPRITWYFIHGPSDASLYYQIAPYTQRLYIKENVPIGSSWKFICSATNSFFERSKTFNKTV 1057
            ++ +L+PP +    + ++ + +TV  N ++ + CQ +DG P  +   VK    +  D      NTH++         +   + D  R V      AG+  KS  + ++ +PT + ++ E V  VL N   + C AT G P P + W+    P  AS    I P  + L I +   +    K+ C A N   E  K    TV
Sbjct: 2847 DVRVLLPPSIR-GADGELPDEVTVLVNKTMLLECQ-VDGSPTPKISWVKDSQPLTQD------NTHRLLSNGRTLQILNAQVTDTGRYVCVAQNLAGTAEKSFNLHVHVSPTIVGIREENVTVVLNNFVSLNCEAT-GLPPPTLRWFKDRRPVQASTNALIMPGGRTLQILK-AKMSDGGKYSCVAVNPAGEAQKLIYLTV 3037

HSP 2 Score: 50.447 bits (119), Expect = 8.420e-6
Identity = 54/202 (26.73%), Postives = 87/202 (43.07%), Query Frame = 2 
Query:  491 NINLLVPPQLELKMNSQVRNYITVEPNSSLTVSCQALDGQPKI-----RPEIVKSDMKIEFDIFSHDSNTHKITIRTSMLDHNRTVTCKA----GSVMKSVRIFIYHAPTKI--KLQPEVKKVLGNNTGIICLATNGYPTPRITWYF--IHGPSDASLYYQIAPYTQRLYIKENVPIGSSWKFICSATNSFFERSKTFNKTV 1057
            ++N+ VPP+L+     +V   I+    + +T +C A    P          ++++   +E  I S  S   K+ I  S L  + T TC A    G   K+  + I+  P  I  +L  E+  +L ++  ++C A  G PTP I W    +     A    +I+P    L +   V    S K+ C ATN   E  + FN  V
Sbjct: 3129 HLNIHVPPRLDGPAEERVVETIS----NPVTFACDATGIPPPSLTWLKNGRVIENSESLEMHILSGGS---KLQISRSQLSDSGTYTCVASNVEGKAQKNYHLVIHVPPNIIGSELPSEMSVLLNDSVQLVCRA-EGTPTPEIQWLKDGMTISRTAQKNIKISPDGSTLTVTA-VHTSDSGKYTCVATNQAGEEDRIFNLNV 3321
[Close]
BLAST of SMED30024938 vs. UniProt/SwissProt
Match: sp|Q9U3P2|SYG2_CAEEL (Synaptogenesis protein syg-2 OS=Caenorhabditis elegans OX=6239 GN=syg-2 PE=1 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 5.672e-7
Identity = 51/191 (26.70%), Postives = 84/191 (43.98%), Query Frame = 2 
Query:  380 QYANNRILEIYYINPNSTKERRNMLNHLKPTQLMYRNNINLLVPPQLELKMNSQVRNYITVEPNSSLTVSCQALDGQPKIRPEIV-KSDMKIEFDIFSHDSNTH---KITIRTSML------DHNRTVTCKAGSVMKSVRI---------FIYHAPTKIKLQPEVKKVLGNNTGIICLATNGYPTPRITWY 895
            Q A    L+I ++N +   E    + H +   +  ++ +N++VPPQL    N Q  + I V+ N+ L ++C   + +P+  PE++   D K+        S  H     T+ TS++      DH + +TC+A      +RI         F    PT   L+       G+N  I C  T G P P + WY
Sbjct:   82 QNAEELHLKITHVNLDDDGEYECQMLHPEEGPIRAKSFLNIIVPPQLVYFSNYQPNSIIAVKENTPLNITCVVPNVKPE--PEVLWYMDGKVMSRDVKQASTPHLNKTFTVYTSLVVQSDRNDHGKVITCEAFQKETDIRITTNTTLDVLFPPSDPTVEILRNPSALRSGDNVTIACSVTGGNPPPDVFWY 270
[Close]
BLAST of SMED30024938 vs. Planmine SMEST
Match: SMESG000014710.1 (SMESG000014710.1)

HSP 1 Score: 1097.03 bits (2836), Expect = 0.000e+0
Identity = 545/547 (99.63%), Postives = 547/547 (100.00%), Query Frame = 2 
Query:  212 MVIQTTDLNGFDKEVAIFDFGLKEVISQEFQSDYMRIPTWGKFEEGATIYTMINFTQYANNRILEIYYINPNSTKERRNMLNHLKPTQLMYRNNINLLVPPQLELKMNSQVRNYITVEPNSSLTVSCQALDGQPKIRPEIVKSDMKIEFDIFSHDSNTHKITIRTSMLDHNRTVTCKAGSVMKSVRIFIYHAPTKIKLQPEVKKVLGNNTGIICLATNGYPTPRITWYFIHGPSDASLYYQIAPYTQRLYIKENVPIGSSWKFICSATNSFFERSKTFNKTVSFTIIHQQRNKTQTYYNTSKIKTSIWDPDQMNYTLKQIEEIKLEIFVRDITITNLKDSIGTLNSEIFQLNQTIQQLRETQRETSSDIKMFKKIFNIEIESXXXXXXXXXXXXIDKNKVNQTQRNSAMTTTPSASLVPIISFDWRINGVSRLRRKEKSFRSVKFVSPKIRFPVTLYFKFRRMESMFISVRMSHINDAESSLVIKGYVTLSIYNAEDVSKQMSTRNFFERNRRSTNVDFVFNYFELHMNQVLQSDDIIVKCSLSISS 1852
            MVIQTTDLNGFDKEVAIFDFGLKEVISQEFQSDYMRIPTWGKFEEGATIYTMINFTQYANNRILEIYYINPNSTKERRNMLNHLKPTQLMYRNNINLLVPPQLELKMNSQVRNYITVEPNSSLTVSCQALDGQPK+RPEIVKSDMKIEFDIFSHDSNTHKITIRTSMLDHNRTVTCKAGSVMKSVRIFIYHAPTKIKLQPEVKKVLGNNTGIICLATNGYPTPRITWYFIHGPSDASLYYQIAPYTQRLYIKENVPIGSSWKFICSATNSFFERSKTFNKTVSFTIIHQQRNKTQTYYNTSKIKTSIWDPDQMNYTLKQIEEIKLEIFVRDITITNLKDSIGTLNSEIFQLNQTIQQLRETQRETSSDIKMFKKIFNIEIESLKKELNNLNGNLIDKNKVNQTQRNSAMTTTPSASLVPIISFDWRINGVSRLRRKEKSFRSVKFV+PKIRFPVTLYFKFRRMESMFISVRMSHINDAESSLVIKGYVTLSIYNAEDVSKQMSTRNFFERNRRSTNVDFVFNYFELHMNQVLQSDDIIVKCSLSISS
Sbjct:    1 MVIQTTDLNGFDKEVAIFDFGLKEVISQEFQSDYMRIPTWGKFEEGATIYTMINFTQYANNRILEIYYINPNSTKERRNMLNHLKPTQLMYRNNINLLVPPQLELKMNSQVRNYITVEPNSSLTVSCQALDGQPKVRPEIVKSDMKIEFDIFSHDSNTHKITIRTSMLDHNRTVTCKAGSVMKSVRIFIYHAPTKIKLQPEVKKVLGNNTGIICLATNGYPTPRITWYFIHGPSDASLYYQIAPYTQRLYIKENVPIGSSWKFICSATNSFFERSKTFNKTVSFTIIHQQRNKTQTYYNTSKIKTSIWDPDQMNYTLKQIEEIKLEIFVRDITITNLKDSIGTLNSEIFQLNQTIQQLRETQRETSSDIKMFKKIFNIEIESLKKELNNLNGNLIDKNKVNQTQRNSAMTTTPSASLVPIISFDWRINGVSRLRRKEKSFRSVKFVNPKIRFPVTLYFKFRRMESMFISVRMSHINDAESSLVIKGYVTLSIYNAEDVSKQMSTRNFFERNRRSTNVDFVFNYFELHMNQVLQSDDIIVKCSLSISS 547
[Close]
BLAST of SMED30024938 vs. Planmine SMEST
Match: SMESG000032651.1 (SMESG000032651.1)

HSP 1 Score: 207.994 bits (528), Expect = 3.330e-59
Identity = 119/349 (34.10%), Postives = 192/349 (55.01%), Query Frame = 2 
Query:   62 LVYCLLSFVLIHLACSQ--FIITNITKFPLPLGRPFEITVSINPSS----GLTLELMVIQTTDLNGFDKEVAIFDFGLKEVISQEFQSDYMRIPTWGKFEEGATIYTMINFTQYANNRILEIYYINPNSTKERRNMLNHLKPTQLMYRNNINLLVPPQLELKMNSQVRNYITVEPNSSLTVSCQALDGQPKIRPEIVKS-DMKIEFDIFSHDSNTHKITIRTSMLDHNRTVTCKAGSVMKSVRIFIYHAPTKIKLQPEVKKVLGNNTGIICLATNGYPTPRITWYFIHGPSDASLYYQIAPYTQRLYIKENVPIGSSWKFICSATNSFFERSKTFNKTVSFTIIHQQRN 1087
            L  CLL   +I ++ +Q    +  I +FP P G  F I +    +     G + ++++IQ +D +   K+ AIF++  K++I  E + D+MRIPTW KF+    I TM+ F + AN++ LEI+ I  ++  E+RN +  L     +Y++ I+LL+ P+LEL + S       +  +     SC+A DG P  +P   K+ D+K+    +S + N   I I  S+LDH + ++C AG    +V+I + H PT+I L PE+   + N T   C + +G+P P + W+FI GP D   Y+ I+   ++L +K   PIG+ WKF C+A+N       +FN+TVSF I   Q  
Sbjct:    9 LSACLLLNAVISISGNQTHLRVNTIPEFPFPAGSNFTIEIVEKENGKQIYGNSHDILIIQISDSS--KKQQAIFEYIYKDMIDMEIKGDFMRIPTWTKFKS-QIIATML-FDRSANDKTLEIFQITASNNNEKRNQMKTLNKNSRIYQSKISLLIDPKLELLITSMDGRKFELMLDEWGKASCRAFDGVPMEKPYWDKNFDLKMRNPKYSPERNERTIEIYASILDHMKELSCTAGRTRAAVKINVLHHPTQILLDPELGSFVENGTTTYCSSIDGFPVPEVRWHFISGPDDPGRYFNISKDGRKLLLKSETPIGTVWKFNCTASNILPRTKGSFNRTVSFEIGRPQEG 353
[Close]
BLAST of SMED30024938 vs. Planmine SMEST
Match: SMESG000014480.1 (SMESG000014480.1)

HSP 1 Score: 179.104 bits (453), Expect = 5.493e-48
Identity = 164/647 (25.35%), Postives = 301/647 (46.52%), Query Frame = 2 
Query:   59 KLVYCLLSFVLIHLA-CSQFIITNITKFPLPLGRPFEITVSI-NPSSGLTLELMVIQTTDLNGFDKEVAIFDFGLKEVISQEFQSDYMRIPTWGKFEEGATIYTMINFTQYANNRILEIYYINPNSTKERRNMLNHLKPTQLMYRNNINLLVPPQLELKMNSQVRNYITVEPNSSLTVSCQALDGQPKIRPEIVK-SDMKIEFDIFSHDSNTHKITIRTSMLDHNRTVTCKAGSVMKSVRIFIYHAPTKIKLQPEVKKVLGNNTGIICLATNGYPTPRITWYFIHGPSDASLYYQIAPYTQRLYIKENVPIGSSWKFICSATNSFFERSKTFNKTVSFTIIHQQRNKTQTYYNTSKIKTSIWDPDQMNYTLKQIEEIKLEIF-VRDITITNLKDSIGTLNSEI-----------FQLNQTIQQLRET----------------------QRETSSDIKMFKKIFNIEIESXXXXXXXXXXXXIDKNKVNQTQRNSAMTTTPSASLVPIISF------DWRINGVSRLRRKE----KSFRSVKFVSPKIRFPVTLYF--KFRRMESMFISVRMSHI--NDAESSLVIKGYVTLSIYNAED-VSKQMSTRNFFERNRRSTNVDFVFNYFELHMNQVLQSDDIIVKCSLS 1843
            K+   +L ++ +  +      I+ I+ FP  + + F IT+S  N S GL  + +VI+ +D  G   + A+FD+G+K+ I   +  ++M +  W K     T+   + F + +N++ +EI  I   S+K +     +   + L+YR+ I LL P +L+L ++S        E       SC+ + G     P  +   +  I+      +    K     S+ DH + + CK+G+ ++  ++ ++H PTK+KL+PE+     NNT + C   NGYP P   W  +HGPS+AS  Y+I    Q L +       S W F CSA NSF    K FNKTV+F II   +NK     N+  IK      +  N+    + +  +E+  V  + +T + + + T N               +LN+ I+QL+++                      +++ S +I+  K+   +  + + +    +N   +DK  ++Q Q+   + T P         F      +W+IN + + R+K      +F    + + K++    L+F   ++   +  ++V +  I   D +S    KG + + IYN+ +    ++ +R+    ++    + F F Y EL ++ VL+SD+II+KC +S
Sbjct:    2 KIAAVILIWIFLKFSKAGSLSISKISPFPHNIHKHFNITLSYSNESFGLG-DTLVIRISDKFGNKFDEAVFDYGVKDEIGDTYSGEFMTVSKWEKNPVEKTLSASLFFNKRSNSQFIEILNIQLISSKFQNLSQENYGESILVYRSKIELLSPTKLKLTVSSVDGKVKEFEEGQIGVASCRLIGGTQNTLPVFINIKNFTIKRSSLILEKTERKFEFLISIADHMKEIICKSGNNIQKAKVKVFHPPTKVKLEPELNNKFVNNTKVSCGHLNGYPIPVAQWRLVHGPSNASTMYEITQGGQHLKVTVPRLDNSIWSFNCSARNSFSNSIKWFNKTVTFFIIENNKNKQ---INSENIKPIRKPENSTNHNELLLIKNTIELLSVNYLKLTKVTEKLITDNKNFSMALKETGKANLRLNREIEQLKKSCSVKTKKLRSNNKKEFFESAMKRKDDSIEIRNLKEGLEVLKKKINEMTELINRTPLDK-AIHQPQQLKLIQTKPPDKTQGYPPFNISPVNEWKINQIQKKRKKAFPIFGNFFYADYNANKMKM---LFFPNGYQTSSATHLTVILQLIFAPDIDSYRPFKGDIIIGIYNSRNQYLNKIISRHLLLTHQVDRAI-FNFKYDELELSGVLKSDEIIIKCFVS 639
[Close]
BLAST of SMED30024938 vs. Planmine SMEST
Match: SMESG000070072.1 (SMESG000070072.1)

HSP 1 Score: 168.703 bits (426), Expect = 4.369e-45
Identity = 101/342 (29.53%), Postives = 172/342 (50.29%), Query Frame = 2 
Query:   65 VYCLLSFVLIHLACSQ--FIITNITKFPLPLGRPFEITVSINPSSGLTL----ELMVIQTTDLNGFDKEVAIFDFGLKEVISQEFQSDYMRIPTWGKFEEGATIYTMINFTQYANNRILEIYYINPNSTKERRNMLNHLKPTQLMYRNNINLLVPPQLELKMNSQVRNYITVEPNSSLTVSCQALDGQPKIRPEIVK-SDMKIEFDIFSHDSNTHKITIRTSMLDHNRTVTCKAGSVMKSVRIFIYHAPTKIKLQPEVKKVLGNNTGIICLATNGYPTPRITWYFIHGPSDASLYYQIAPYTQRLYIKENVPIGSSWKFICSATNSFFERSKTFNKTVSFTI 1069
            +YC   F+ + LA SQ    + +++KFP  +G  F + +     +G       + M I+  DLN  + E A   +G+   +  EF+   M +  W K      +   I F + A+N+ LEI+Y + ++  ER+  L +L   + ++   I+L +P  L L +     +   +  N  +  SC+A  G P+ +P     + + +   I++  +N  ++ +R S+ DH    +C AG+V ++  I I+H PT + +QP +KK   +N+ I C   +GYP P I W  I GP +A+L+Y I P   ++YI  N   GS W F C+  +     +K  NKT+SF I
Sbjct:    6 LYCF--FLPLILAQSQIPLFVKSVSKFPHLIGDAFSLDIQSRTENGKLSSDPSDTMAIRIVDLNFTEIERAFLVYGVLNFMDDEFKGKLMDVKVWEKNPTLTAMNISIIFNRAAHNKYLEIFYFSASNALERKKYLKNLSLAKRLFHELIDLFIPSTLTLSVLPIKVDRREIIINDMVMASCKAFGGIPQEKPRFEDHAHLNVTEIIYNKTANEIRMNLRASLTDHLTNFSCVAGNVTETFTIRIHHHPTALYIQP-IKKRFQSNSTIKCYTNDGYPDPEINWQLIEGPPNANLFYNIPPKGDQIYINSNSQTGSKWTFRCTMKSFLVNTNKILNKTISFAI 344
[Close]
BLAST of SMED30024938 vs. Planmine SMEST
Match: SMESG000014496.1 (SMESG000014496.1)

HSP 1 Score: 169.088 bits (427), Expect = 6.976e-45
Identity = 98/345 (28.41%), Postives = 171/345 (49.57%), Query Frame = 2 
Query:   53 MTKLVYCLLSFVLIHLACSQFIITNITKFPLPLGRPFEITVSINPSSGLTL-----ELMVIQTTDLNGFDKEVAIFDFGLKEVISQEFQSDYMRIPTWGKFEEGATIYTMINFTQYANNRILEIYYINPNSTKERRNMLNHLKPTQLMYRNNINLLVPPQLELKMNSQVRNYITVEPNSSLTVSCQALDGQPKIRPEIVKS-DMKIEFDIFSHDSNTHKITIRTSMLDHNRTVTCKAGSVMKSVRIFIYHAPTKIKLQPEVKKVLGNNTGIICLATNGYPTPRITWYFIHGPSDASLYYQIAPYTQRLYIKENVPIGSSWKFICSATNSFFERSKTFNKTVSFTI 1069
            +  +++CL+  +++  A     +  ++  P  L   F+I +    ++G  L     + MVI+    N  ++E +I ++G ++ I +E    +M I  W K      I   I F + A+N+ LEI+Y +  +  +RR  L +L   + +    I L +P QL L M     +   +  N  +  SC+A  G P+ +P+      + +   I++  +N  ++ +R S+ DH    +C AG+V ++  I I+H PT + +QP +KK   +N+ IIC   +GYP P I W  I GP +A  +Y       +++I  N P+GS W F C+  +      K  NKT+SF I
Sbjct:    3 LNAVIFCLIQQLVLTDAQPLLTVCFVSTVPHLLKNTFDIQIKPQ-ANGTKLSDDPSDTMVIRIYSFNFTEEEKSILNYGSQDAIDEEILGKFMDIKVWEKNFSSLRINISIMFKRGAHNKYLEIFYFSAPNKFQRRKYLKNLSLAKRLLYQKIELFMPSQLNLSMYPLNVDRKEIIINDMVMASCRAFGGIPQEKPKFEDHVHLNVMEKIYNKTTNEIRMNLRASLSDHLTNFSCSAGNVTETYTIRIHHHPTALYIQPIIKKRFRSNSTIICFTKDGYPDPEINWQLIEGPPNADRFYNFPGKGDQIHINSNSPVGSKWTFKCTMKSMLVNTKKMLNKTISFAI 346
The following BLAST results are available for this feature:
BLAST of SMED30024938 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024938 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
syg-24.651e-826.70Synaptogenesis protein syg-2 [Source:UniProtKB/Sw... [more]
syg-25.254e-826.70Synaptogenesis protein syg-2 [Source:UniProtKB/Sw... [more]
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BLAST of SMED30024938 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024938 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
hmcn15.066e-726.37hemicentin 1 [Source:ZFIN;Acc:ZDB-GENE-041014-322][more]
hmcn15.066e-726.37hemicentin 1 [Source:ZFIN;Acc:ZDB-GENE-041014-322][more]
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BLAST of SMED30024938 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024938 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024938 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|Q9U3P2|SYG2_CAEEL5.672e-726.70Synaptogenesis protein syg-2 OS=Caenorhabditis ele... [more]
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BLAST of SMED30024938 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024938 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024938 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024938 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024938 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024938 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024938 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000014710.10.000e+099.63SMESG000014710.1[more]
SMESG000032651.13.330e-5934.10SMESG000032651.1[more]
SMESG000014480.15.493e-4825.35SMESG000014480.1[more]
SMESG000070072.14.369e-4529.53SMESG000070072.1[more]
SMESG000014496.16.976e-4528.41SMESG000014496.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024938 ID=SMED30024938|Name=SMED30024938|organism=Schmidtea mediterranea sexual|type=transcript|length=1926bp
TATACATTTACTCGTTTCAATTTTGTTCATTTTATTATTGTGTGAAGTTT
AAATGACAAAATTAGTATATTGCTTATTAAGTTTTGTGCTGATTCATTTG
GCATGCAGCCAATTTATAATTACAAATATCACCAAATTTCCTTTACCCTT
AGGGAGACCTTTTGAGATCACAGTGTCCATAAATCCTAGTTCAGGTTTGA
CACTGGAGTTAATGGTAATTCAAACAACCGATTTAAATGGATTTGATAAG
GAAGTTGCAATTTTTGACTTCGGACTCAAAGAGGTCATATCCCAAGAATT
TCAAAGTGACTACATGAGAATACCAACTTGGGGAAAATTTGAGGAAGGCG
CAACCATTTATACTATGATTAATTTTACACAATATGCCAACAACCGGATC
CTTGAGATTTATTATATAAATCCGAATTCCACCAAGGAACGGCGAAATAT
GCTCAATCATCTGAAACCAACTCAACTGATGTATCGCAACAATATAAACT
TGTTAGTACCGCCACAGCTGGAATTGAAAATGAACTCTCAAGTACGCAAT
TATATCACAGTGGAACCCAATTCATCATTGACCGTGTCATGCCAAGCGCT
CGACGGTCAGCCAAAGATCCGTCCAGAAATTGTTAAAAGTGATATGAAGA
TTGAATTCGACATATTCTCTCATGACTCCAATACACACAAAATTACAATA
CGTACTTCAATGCTTGATCATAATCGGACAGTGACATGCAAAGCTGGAAG
TGTTATGAAATCCGTTAGGATTTTTATATATCATGCTCCAACAAAGATTA
AACTACAACCGGAAGTTAAGAAAGTTCTGGGAAATAACACTGGAATCATC
TGTTTGGCTACAAACGGATATCCAACTCCAAGGATAACATGGTATTTTAT
TCACGGTCCGTCAGATGCATCGTTATATTATCAAATTGCTCCGTATACAC
AGCGTCTTTATATTAAGGAAAATGTTCCAATTGGATCTTCATGGAAATTC
ATCTGCAGTGCTACAAATTCATTTTTTGAAAGGTCTAAAACATTCAACAA
GACCGTCTCCTTTACCATTATACATCAGCAAAGAAACAAGACTCAGACAT
ATTATAATACTTCCAAGATCAAAACTAGTATCTGGGATCCTGATCAAATG
AATTATACATTAAAACAAATAGAAGAAATTAAGCTAGAAATTTTCGTGAG
AGACATAACCATCACAAATTTAAAGGATTCCATTGGCACTTTGAATTCGG
AAATATTCCAATTAAACCAGACAATACAGCAGTTGCGGGAAACCCAAAGG
GAAACAAGTAGCGATATAAAAATGTTTAAAAAGATTTTTAACATAGAGAT
TGAAAGTTTAAAGAAGGAACTGAATAACTTGAATGGAAATTTGATTGACA
AAAATAAAGTAAACCAAACACAGCGGAATTCGGCAATGACAACAACTCCA
AGCGCATCTCTCGTACCAATCATTTCATTCGATTGGAGAATAAATGGCGT
TTCTCGTCTTCGACGAAAAGAAAAGTCTTTCCGTTCGGTGAAATTCGTAA
GTCCCAAAATTCGATTTCCGGTGACTTTATATTTCAAATTCCGCCGAATG
GAGAGCATGTTCATTTCCGTGCGTATGTCACACATAAATGATGCAGAATC
GAGTTTGGTTATCAAAGGATATGTAACGCTTTCTATTTACAATGCTGAAG
ATGTTTCAAAGCAAATGTCGACGAGGAATTTCTTTGAACGAAATCGAAGA
TCCACAAATGTCGATTTTGTTTTCAACTATTTCGAGTTGCATATGAATCA
AGTTTTACAAAGTGACGACATTATAGTGAAATGTTCCTTGAGTATTTCCA
GTTAGACCAAAAAGGACATACGAAGAGATTTTACGACGAAAACATTTTAA
TAAAATGTATTTCTTTTGAAAAAAAA
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protein sequence of SMED30024938-orf-1

>SMED30024938-orf-1 ID=SMED30024938-orf-1|Name=SMED30024938-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=601bp
MTKLVYCLLSFVLIHLACSQFIITNITKFPLPLGRPFEITVSINPSSGLT
LELMVIQTTDLNGFDKEVAIFDFGLKEVISQEFQSDYMRIPTWGKFEEGA
TIYTMINFTQYANNRILEIYYINPNSTKERRNMLNHLKPTQLMYRNNINL
LVPPQLELKMNSQVRNYITVEPNSSLTVSCQALDGQPKIRPEIVKSDMKI
EFDIFSHDSNTHKITIRTSMLDHNRTVTCKAGSVMKSVRIFIYHAPTKIK
LQPEVKKVLGNNTGIICLATNGYPTPRITWYFIHGPSDASLYYQIAPYTQ
RLYIKENVPIGSSWKFICSATNSFFERSKTFNKTVSFTIIHQQRNKTQTY
YNTSKIKTSIWDPDQMNYTLKQIEEIKLEIFVRDITITNLKDSIGTLNSE
IFQLNQTIQQLRETQRETSSDIKMFKKIFNIEIESLKKELNNLNGNLIDK
NKVNQTQRNSAMTTTPSASLVPIISFDWRINGVSRLRRKEKSFRSVKFVS
PKIRFPVTLYFKFRRMESMFISVRMSHINDAESSLVIKGYVTLSIYNAED
VSKQMSTRNFFERNRRSTNVDFVFNYFELHMNQVLQSDDIIVKCSLSISS
*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0002075pigment cell
Vocabulary: INTERPRO
TermDefinition
IPR008974TRAF-like
IPR036179Ig-like_dom_sf
IPR013783Ig-like_fold
IPR007110Ig-like_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0042803protein homodimerization activity
Vocabulary: cellular component
TermDefinition
GO:0005615extracellular space
GO:0005886plasma membrane
GO:0009986cell surface
GO:0016020membrane
GO:0016021integral component of membrane
GO:0030054cell junction
GO:0045202synapse
Vocabulary: biological process
TermDefinition
GO:0007155cell adhesion
GO:0007156homophilic cell adhesion via plasma membrane adhesion molecules
GO:0007399nervous system development
GO:0007416synapse assembly
GO:0007417central nervous system development
GO:0007420brain development
GO:0010842retina layer formation
GO:0048704embryonic skeletal system morphogenesis
GO:0060219camera-type eye photoreceptor cell differentiation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 426..453
NoneNo IPR availableCOILSCoilCoilcoord: 394..414
NoneNo IPR availableCDDcd00096Igcoord: 265..332
e-value: 3.56654E-4
score: 37.2217
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..19
score: 0.878
NoneNo IPR availableTMHMMTMhelixcoord: 5..27
IPR008974TRAF-likeGENE3DG3DSA:2.60.210.10coord: 473..599
e-value: 2.7E-5
score: 26.3
IPR013783Immunoglobulin-like foldGENE3DG3DSA:2.60.40.10coord: 245..347
e-value: 2.4E-7
score: 32.6
IPR007110Immunoglobulin-like domainPROSITEPS50835IG_LIKEcoord: 246..336
score: 8.938
IPR036179Immunoglobulin-like domain superfamilySUPERFAMILYSSF48726Immunoglobulincoord: 243..335
IPR036179Immunoglobulin-like domain superfamilySUPERFAMILYSSF48726Immunoglobulincoord: 151..235