SMED30024864
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30024864 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7
Alignments
SMED30024864 aligns in the following genomic locations:
Homology
BLAST of SMED30024864 vs. Planmine SMEST
Match: SMESG000011359.1 (SMESG000011359.1) HSP 1 Score: 736.873 bits (1901), Expect = 0.000e+0 Identity = 361/368 (98.10%), Postives = 363/368 (98.64%), Query Frame = 3 Query: 138 MQFLQLYLSVMVLIEFTEIVTTTLIGKFNGTLGDVRQLEMSMEFIAPSNTVLMWAVDSDGNLFIIKTGIQYQQNKQIECIINKNRFNITSSSIQLGKQNFQKLQWSTEIFHKEFGHSPVFESSMRNIHLNKESRKVINKKMKSNLTLILIGSEVAHKSPISNRKPLFGCLSKFSINGMQIIFDRNGNDFAQNCMKFIKEIILQMNRYAPRNFSLHFLDCGVTSLNTNELCYRDYVVHSHEIHFYELLDGKDQKIIKLIWKPIFSLNECLIEIQTINNSFFLIYKNHKNEIWRSLNGKAWIIHKILSETRYVLLINFQSRNINLQIANESVSYNFNENINIFPKYIHVGFSSQKTLCENLTGEYAIQFE 1241 MQFLQLYLSV+VLIEFTEIVTTTLIGKFNGTLGDVRQLEMSMEFIAPSNTVLMWAVDSDGNLFIIKTGIQYQQNKQIECIINKNRFNITSSSIQ GKQNFQKLQWSTEIFHKE GHSPVFESSMRNIHLNKESRKVINKKMKSNLTLILIGSEVAHK+PISNRKPLFGCLSKFSING QIIFDRNGNDFAQNCMKFIKEIILQMNRYAPRNFSL FLDCGVTSLNTNELCYRDYVVHSHEIHFYELLDGKDQKIIKLIWKPIFSLNECLIEIQTINNSFFLIYKNHKNEIWRSLNGKAWIIHKILSETRYVLLINFQSRNINLQIANESVSYNFNENINIFPKYIHVGFSSQ TLCENLTGEYAIQFE Sbjct: 1 MQFLQLYLSVIVLIEFTEIVTTTLIGKFNGTLGDVRQLEMSMEFIAPSNTVLMWAVDSDGNLFIIKTGIQYQQNKQIECIINKNRFNITSSSIQSGKQNFQKLQWSTEIFHKELGHSPVFESSMRNIHLNKESRKVINKKMKSNLTLILIGSEVAHKAPISNRKPLFGCLSKFSINGRQIIFDRNGNDFAQNCMKFIKEIILQMNRYAPRNFSLQFLDCGVTSLNTNELCYRDYVVHSHEIHFYELLDGKDQKIIKLIWKPIFSLNECLIEIQTINNSFFLIYKNHKNEIWRSLNGKAWIIHKILSETRYVLLINFQSRNINLQIANESVSYNFNENINIFPKYIHVGFSSQNTLCENLTGEYAIQFE 368
BLAST of SMED30024864 vs. Planmine SMEST
Match: SMESG000011359.1 (SMESG000011359.1) HSP 1 Score: 728.013 bits (1878), Expect = 0.000e+0 Identity = 359/368 (97.55%), Postives = 360/368 (97.83%), Query Frame = 3 Query: 138 MQFLQLYLSVMVLIEFTEIVTTTLIGKFNGTLGDVRQLEMSMEFIAPSNTVLMWAVDSDGNLFIIKTGIQYQQNKQIECIINKNRFNITSSSIQLGKQNFQKLQWSTEIFHKEFGHSPVFESSMRNIHLNKESRKVINKKMKSNLTLILIGSEVAHKSPISNRKPLFGCLSKFSINGMQIIFDRNGNDFAQNCMKFIKEIILQMNRYAPRNFSLHFLDCGVTSLNTNELCYRDYVVHSHEIHFYELLDGKDQKIIKLIWKPIFSLNECLIEIQTINNSFFLIYKNHKNEIWRSLNGKAWIIHKILSETRYVLLINFQSRNINLQIANESVSYNFNENINIFPKYIHVGFSSQKTLCENLTGEYAIQFE 1241 MQFLQLYLSV IEFTEIVTTTLIGKFNGTLGDVRQLEMSMEFIAPSNTVLMWAVDSDGNLFIIKTGIQYQQNKQIECIINKNRFNITSSSIQ GKQNFQKLQWSTEIFHKE GHSPVFESSMRNIHLNKESRKVINKKMKSNLTLILIGSEVAHK+PISNRKPLFGCLSKFSING QIIFDRNGNDFAQNCMKFIKEIILQMNRYAPRNFSL FLDCGVTSLNTNELCYRDYVVHSHEIHFYELLDGKDQKIIKLIWKPIFSLNECLIEIQTINNSFFLIYKNHKNEIWRSLNGKAWIIHKILSETRYVLLINFQSRNINLQIANESVSYNFNENINIFPKYIHVGFSSQ TLCENLTGEYAIQFE Sbjct: 1 MQFLQLYLSV---IEFTEIVTTTLIGKFNGTLGDVRQLEMSMEFIAPSNTVLMWAVDSDGNLFIIKTGIQYQQNKQIECIINKNRFNITSSSIQSGKQNFQKLQWSTEIFHKELGHSPVFESSMRNIHLNKESRKVINKKMKSNLTLILIGSEVAHKAPISNRKPLFGCLSKFSINGRQIIFDRNGNDFAQNCMKFIKEIILQMNRYAPRNFSLQFLDCGVTSLNTNELCYRDYVVHSHEIHFYELLDGKDQKIIKLIWKPIFSLNECLIEIQTINNSFFLIYKNHKNEIWRSLNGKAWIIHKILSETRYVLLINFQSRNINLQIANESVSYNFNENINIFPKYIHVGFSSQNTLCENLTGEYAIQFE 365
BLAST of SMED30024864 vs. Planmine SMEST
Match: SMESG000056437.1 (SMESG000056437.1) HSP 1 Score: 114.39 bits (285), Expect = 4.138e-27 Identity = 97/369 (26.29%), Postives = 169/369 (45.80%), Query Frame = 3 Query: 207 LIGKFNGTLGDVRQLEMSMEFIAPSNTVLMWAVDSDGNLFIIKTGIQYQQNKQIECIINKNRFNITSSSIQL-GKQNFQK-LQWSTE---IFHKEFGHSPVFESSMRNIHLNKE-------SRKVINKKMKSNLTLILIGSEVAHKSPI--SNRKPLFGCLSKFSINGMQIIFDRNGNDFAQNCMKFIKEIILQMNRYAPRNFSLHFLDCGVTSLNTNELCYRDYVVH-SHEIHFYELLDGKDQKIIKLIWKPIFSLNECLIEIQTINNSFFLIYKNHKNEIW------RSLNGKAWIIHKILSETRYVLLINFQSRNINLQIANESVSYNFNENINIFPKY---IHVGFSSQKTLCENLTGEYAIQFE 1241 L GK + + Q + ++F S+ +L++A+D + N F+I++ +Y+ ++I N FN T++ + G F LQ+ + I + H+ + RN N + S + ++ L +G + + ++ + GC+ F IN +I F G+D C K E I + + LDC ++ +NT E CY D++ + + + + G++ +I+K++WK FS + CL+EI+T S L+Y N NEI RS+ + K+ +VLLI F N L+I+N S F E+ +P Y I V + S + LC G+ A Q+E Sbjct: 25 LYGKLDENFENGMQFYIDLDFRDVSHGILLYAIDRNRNRFLIESAEKYRGRRKIVFNFNGQIFNSTNTYWRFSGTSTFTIFLQFKYDNLYIMDYSYRHATLLNVFNRN-KTNPDTFVGYPISDDHNSTTRTTDFWLGNVGPSIGKDKTVFQPSKVQMVGCVYNFRINYKEIKFTTYGDDLLPFCRKQFSETICIKGKCKKEQRRSYILDCSLSEMNTKEYCYEDFLTYMGQTLSYLTVGLGEEPRIMKMVWKQEFSKDYCLLEIETKKESSILVYANRLNEIVLNIRSNRSVEWTRRTLCKMQPNLIHVLLIEFVYPNFVLKISNGSNVLTFIEH---YPDYIIKIDVAYKSHRNLCSTFEGQLAFQYE 389
BLAST of SMED30024864 vs. Planmine SMEST
Match: SMESG000056437.1 (SMESG000056437.1) HSP 1 Score: 114.39 bits (285), Expect = 4.337e-27 Identity = 97/369 (26.29%), Postives = 169/369 (45.80%), Query Frame = 3 Query: 207 LIGKFNGTLGDVRQLEMSMEFIAPSNTVLMWAVDSDGNLFIIKTGIQYQQNKQIECIINKNRFNITSSSIQL-GKQNFQK-LQWSTE---IFHKEFGHSPVFESSMRNIHLNKE-------SRKVINKKMKSNLTLILIGSEVAHKSPI--SNRKPLFGCLSKFSINGMQIIFDRNGNDFAQNCMKFIKEIILQMNRYAPRNFSLHFLDCGVTSLNTNELCYRDYVVH-SHEIHFYELLDGKDQKIIKLIWKPIFSLNECLIEIQTINNSFFLIYKNHKNEIW------RSLNGKAWIIHKILSETRYVLLINFQSRNINLQIANESVSYNFNENINIFPKY---IHVGFSSQKTLCENLTGEYAIQFE 1241 L GK + + Q + ++F S+ +L++A+D + N F+I++ +Y+ ++I N FN T++ + G F LQ+ + I + H+ + RN N + S + ++ L +G + + ++ + GC+ F IN +I F G+D C K E I + + LDC ++ +NT E CY D++ + + + + G++ +I+K++WK FS + CL+EI+T S L+Y N NEI RS+ + K+ +VLLI F N L+I+N S F E+ +P Y I V + S + LC G+ A Q+E Sbjct: 26 LYGKLDENFENGMQFYIDLDFRDVSHGILLYAIDRNRNRFLIESAEKYRGRRKIVFNFNGQIFNSTNTYWRFSGTSTFTIFLQFKYDNLYIMDYSYRHATLLNVFNRN-KTNPDTFVGYPISDDHNSTTRTTDFWLGNVGPSIGKDKTVFQPSKVQMVGCVYNFRINYKEIKFTTYGDDLLPFCRKQFSETICIKGKCKKEQRRSYILDCSLSEMNTKEYCYEDFLTYMGQTLSYLTVGLGEEPRIMKMVWKQEFSKDYCLLEIETKKESSILVYANRLNEIVLNIRSNRSVEWTRRTLCKMQPNLIHVLLIEFVYPNFVLKISNGSNVLTFIEH---YPDYIIKIDVAYKSHRNLCSTFEGQLAFQYE 390
BLAST of SMED30024864 vs. Planmine SMEST
Match: SMESG000050489.1 (SMESG000050489.1) HSP 1 Score: 106.301 bits (264), Expect = 2.319e-24 Identity = 92/370 (24.86%), Postives = 166/370 (44.86%), Query Frame = 3 Query: 168 MVLIEFTEIVTTTLIGKFNGTLGDVRQLEMSMEFIAPSNTVLMWAVDSDGNLFIIKTGIQYQQNKQIECIINKNRFNITSSSIQLGKQNFQKLQWSTEIFHKEFGHSPVFESSMRN-IHLNKESRKVINKKMKSNLTLILIGSEVAHKSPIS-----NRKPLFGCLSKFSINGMQIIFDRNGNDFAQNCMKFIKEIILQMNRYAPRNFSLHFLDCGVTSLNTNELCYRDYVVHSHEIHFYELLDGK-DQKIIKLIWKPIFSLNECLIEIQTINNSFFLIYKNHKNEIW------RSLNGKAWIIHKILSETRYVLLINFQSRNINLQIANESVSYNFNENINIFPKYIHVGFSSQKTLCENLTGEYAIQF 1238 +++ F + T+ L G+F T + L + +F SN +++++ D N F +KTG + NKQ+ N +T+S+ G K + + + S F ++ N + N+ MK T I +G +P+ N + GCL + +N ++ ++ G+ F C E + H LDC ++SL+ +C D+++ ++ +G ++K++KLIWK N CL+EI T NNS L Y N+ NE+ +SL + + + ++ YV+ + + N+ ++I S F E +N K++ VGF SQ+ +CE T A + Sbjct: 15 VLICGFVRLSTSYLSGQFADTFQNGINLTVIFDFNPISNGLVLYSSDRYRNYFWVKTGENFINNKQMNGTFNGVEIKLTNSTESHG---ISKCSINVYLKADKMNKSDSFNTTNSNSTEADLSEADFENQTMK---TYIWLGYFDRSSNPMGSLNWDNLTEITGCLLELRVNNKAVVTNQIGSHFTNACYVNYTETVCTGGNCTSGMKEKHILDCSISSLSRERICNLDFLLKMPTNRSFDSPEGSTNEKLMKLIWKLNGPSNYCLMEIGTRNNSVILFYVNNLNELLMCMRNSQSLKCSSRSLVTLTTDEIYVMTVGYAPPNLTVKIPFGSGVKTFVETVNNDIKWVKVGFRSQENVCERFTENIAFVY 378 The following BLAST results are available for this feature:
BLAST of SMED30024864 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30024864 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30024864 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30024864 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30024864 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30024864 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30024864 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30024864 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30024864 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30024864 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30024864 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30024864 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30024864 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30024864 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30024864 ID=SMED30024864|Name=SMED30024864|organism=Schmidtea mediterranea sexual|type=transcript|length=1517bpback to top protein sequence of SMED30024864-orf-1 >SMED30024864-orf-1 ID=SMED30024864-orf-1|Name=SMED30024864-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=451bp MQFLQLYLSVMVLIEFTEIVTTTLIGKFNGTLGDVRQLEMSMEFIAPSNTback to top Annotated Terms
The following terms have been associated with this transcript:
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