Repressor of RNA polymerase III transcription MAF1-like protein

Overview
NameRepressor of RNA polymerase III transcription MAF1-like protein
Smed IDSMED30024845
Length (bp)689
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Repressor of RNA polymerase III transcription MAF1-like protein (SMED30024845) t-SNE clustered cells

Violin plots show distribution of expression levels for Repressor of RNA polymerase III transcription MAF1-like protein (SMED30024845) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Repressor of RNA polymerase III transcription MAF1-like protein (SMED30024845) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Repressor of RNA polymerase III transcription MAF1-like protein (SMED30024845) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30024845

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30024845SMESG000006493.1 SMESG000006492.1 SMESG000006491.1 dd_Smed_v4_2424_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30024845SMESG000006493.1 SMESG000006492.1 SMESG000006491.1 dd_Smed_v4_2424_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30024845SMESG000006493.1 SMESG000006492.1 SMESG000006491.1 dd_Smed_v4_2424_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Human
Match: MAF1 (MAF1 homolog, negative regulator of RNA polymerase III [Source:HGNC Symbol;Acc:HGNC:24966])

HSP 1 Score: 71.2478 bits (173), Expect = 3.470e-14
Identity = 52/177 (29.38%), Postives = 81/177 (45.76%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQCDSYR-QRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYVDLYYK-IRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNN-NGINFLFYNDNLKRLLVFTIK 614
            RIE YSCKM   +K  +K  C          LS PQ       RL+      +   +    + K   +L + L+  +R  YD+S   S +F  E S   VV   +  + + V   +K ++ ++W  +D+ I L  C IYSY  +   DP   D +    N+ FYN  LKR++ F+ +
Sbjct:   28 RIESYSCKMAGDDKHMFKQFCQEGQPHVLEALSPPQTSGLSPSRLSKSQGGEEEGPLSDKCSRKTLFYLIATLNESFRPDYDFSTARSHEFSREPSLSWVVNAVNCSLFSAVREDFKDLKPQLWNAVDEEICLAECDIYSYNPDLDSDPFGEDGSLWSFNYFFYNKRLKRIVFFSCR 204
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Human
Match: MAF1 (MAF1 homolog, negative regulator of RNA polymerase III [Source:HGNC Symbol;Acc:HGNC:24966])

HSP 1 Score: 71.2478 bits (173), Expect = 3.470e-14
Identity = 52/177 (29.38%), Postives = 81/177 (45.76%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQCDSYR-QRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYVDLYYK-IRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNN-NGINFLFYNDNLKRLLVFTIK 614
            RIE YSCKM   +K  +K  C          LS PQ       RL+      +   +    + K   +L + L+  +R  YD+S   S +F  E S   VV   +  + + V   +K ++ ++W  +D+ I L  C IYSY  +   DP   D +    N+ FYN  LKR++ F+ +
Sbjct:   28 RIESYSCKMAGDDKHMFKQFCQEGQPHVLEALSPPQTSGLSPSRLSKSQGGEEEGPLSDKCSRKTLFYLIATLNESFRPDYDFSTARSHEFSREPSLSWVVNAVNCSLFSAVREDFKDLKPQLWNAVDEEICLAECDIYSYNPDLDSDPFGEDGSLWSFNYFFYNKRLKRIVFFSCR 204
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Human
Match: MAF1 (MAF1 homolog, negative regulator of RNA polymerase III [Source:HGNC Symbol;Acc:HGNC:24966])

HSP 1 Score: 55.4546 bits (132), Expect = 1.186e-8
Identity = 52/207 (25.12%), Postives = 81/207 (39.13%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQCDSYR-QRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYVDLYYK-IRHEMWVEIDKIINLKLCQIY------------------------------SYKSNKIDDPLQIDNN-NGINFLFYNDNLKRLLVFTIK 614
            RIE YSCKM   +K  +K  C          LS PQ       RL+      +   +    + K   +L + L+  +R  YD+S   S +F  E S   VV   +  + + V   +K ++ ++W  +D+ I L  C IY                              SY  +   DP   D +    N+ FYN  LKR++ F+ +
Sbjct:   28 RIESYSCKMAGDDKHMFKQFCQEGQPHVLEALSPPQTSGLSPSRLSKSQGGEEEGPLSDKCSRKTLFYLIATLNESFRPDYDFSTARSHEFSREPSLSWVVNAVNCSLFSAVREDFKDLKPQLWNAVDEEICLAECDIYRWAGIPADGLQASRFVGHLAPHTTCHPILPSYNPDLDSDPFGEDGSLWSFNYFFYNKRLKRIVFFSCR 234
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Human
Match: MAF1 (MAF1 homolog, negative regulator of RNA polymerase III [Source:HGNC Symbol;Acc:HGNC:24966])

HSP 1 Score: 55.0694 bits (131), Expect = 1.626e-8
Identity = 51/207 (24.64%), Postives = 80/207 (38.65%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQCDSYR-QRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYVDLYYK-IRHEMWVEIDKIINLKLCQIYSYKSNKID------------------------------DPLQIDNN-NGINFLFYNDNLKRLLVFTIK 614
            RIE YSCKM   +K  +K  C          LS PQ       RL+      +   +    + K   +L + L+  +R  YD+S   S +F  E S   VV   +  + + V   +K ++ ++W  +D+ I L  C IY +     D                              DP   D +    N+ FYN  LKR++ F+ +
Sbjct:   28 RIESYSCKMAGDDKHMFKQFCQEGQPHVLEALSPPQTSGLSPSRLSKSQGGEEEGPLSDKCSRKTLFYLIATLNESFRPDYDFSTARSHEFSREPSLSWVVNAVNCSLFSAVREDFKDLKPQLWNAVDEEICLAECDIYRWAGIPADGLQASRFVGHLAPHTTCHPILPSYNPDLDSDPFGEDGSLWSFNYFFYNKRLKRIVFFSCR 234
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Celegans
Match: mafr-1 (Repressor of RNA polymerase III transcription MAF1 [Source:UniProtKB/TrEMBL;Acc:Q9TZN2])

HSP 1 Score: 63.5438 bits (153), Expect = 5.337e-12
Identity = 57/196 (29.08%), Postives = 86/196 (43.88%), Query Frame = 3 
Query:   87 DIRIECYSCKMTQKEKRHYKSLCNT--------FGSDANFPLSHPQCDSYRQRL-----------TSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYVDLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDN-NNGINFLFYNDNLKRLLVFTIK 614
            D ++E YS KM   EK+ +KS   +         GS     +S      +  RL           +    ++    +K  I+ K    LT  L+ C    +D+S   S  F    +  ++ +  D  +ET V  Y+  R E+W  ID+ I    CQIYS+KS   DDP   D     + F+FYN  LKR ++ TI+
Sbjct:   26 DFKLETYSSKMVTSEKKQWKSNDKSVIWGERQPLGSYEEMVMSASPSVGHNHRLRHLSERSCSGGSDNDFDVNDYLIKDSISRKKLYDLTQVLN-CSFPDHDFSNANSEAFAL-VNYSDLSRLVDMKLETIVRDYHVRREELWGIIDEAIVPGDCQIYSFKSQFEDDPFTEDGCIWALAFIFYNKGLKRFVLLTIR 219
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Celegans
Match: mafr-1 (Repressor of RNA polymerase III transcription MAF1 [Source:UniProtKB/TrEMBL;Acc:Q9TZN2])

HSP 1 Score: 63.5438 bits (153), Expect = 5.337e-12
Identity = 57/196 (29.08%), Postives = 86/196 (43.88%), Query Frame = 3 
Query:   87 DIRIECYSCKMTQKEKRHYKSLCNT--------FGSDANFPLSHPQCDSYRQRL-----------TSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYVDLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDN-NNGINFLFYNDNLKRLLVFTIK 614
            D ++E YS KM   EK+ +KS   +         GS     +S      +  RL           +    ++    +K  I+ K    LT  L+ C    +D+S   S  F    +  ++ +  D  +ET V  Y+  R E+W  ID+ I    CQIYS+KS   DDP   D     + F+FYN  LKR ++ TI+
Sbjct:   26 DFKLETYSSKMVTSEKKQWKSNDKSVIWGERQPLGSYEEMVMSASPSVGHNHRLRHLSERSCSGGSDNDFDVNDYLIKDSISRKKLYDLTQVLN-CSFPDHDFSNANSEAFAL-VNYSDLSRLVDMKLETIVRDYHVRREELWGIIDEAIVPGDCQIYSFKSQFEDDPFTEDGCIWALAFIFYNKGLKRFVLLTIR 219
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Fly
Match: Maf1 (gene:FBgn0267861 transcript:FBtr0301121)

HSP 1 Score: 68.1662 bits (165), Expect = 1.187e-13
Identity = 57/188 (30.32%), Postives = 87/188 (46.28%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSL-CNTFGSDANFPLSHPQ-----CDSYRQRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYV-DLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNNNG----INFLFYNDNLKRLLVFTIKFYN 623
            RIE YSCKM   EK  YK    ++ G D    LS PQ       ++R+  +    E   +++   I+ K   +L + L+  +   YD+S+ +S +F  E S + V+ +    +     D Y  IR  +W  +D  + L  C IYSY  +   DP       G     N+ FYN  LKR++ FT +  N
Sbjct:   28 RIESYSCKMVAAEKVLYKRFTADSHGHDLQ-ALSPPQTLADFSPNFRRNNSQSGDEG--ITLCDTISRKTLFYLIATLNASFEPDYDFSEAKSHEFSKEPSLQWVMNSIHANLSALAGDKYQAIRQPLWSAVDDEVILSECDIYSYNPDLSCDPF---GEPGCLWSFNYFFYNKKLKRIVFFTSRAVN 209
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Fly
Match: Maf1 (gene:FBgn0267861 transcript:FBtr0113830)

HSP 1 Score: 68.1662 bits (165), Expect = 1.187e-13
Identity = 57/188 (30.32%), Postives = 87/188 (46.28%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSL-CNTFGSDANFPLSHPQ-----CDSYRQRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYV-DLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNNNG----INFLFYNDNLKRLLVFTIKFYN 623
            RIE YSCKM   EK  YK    ++ G D    LS PQ       ++R+  +    E   +++   I+ K   +L + L+  +   YD+S+ +S +F  E S + V+ +    +     D Y  IR  +W  +D  + L  C IYSY  +   DP       G     N+ FYN  LKR++ FT +  N
Sbjct:   28 RIESYSCKMVAAEKVLYKRFTADSHGHDLQ-ALSPPQTLADFSPNFRRNNSQSGDEG--ITLCDTISRKTLFYLIATLNASFEPDYDFSEAKSHEFSKEPSLQWVMNSIHANLSALAGDKYQAIRQPLWSAVDDEVILSECDIYSYNPDLSCDPF---GEPGCLWSFNYFFYNKKLKRIVFFTSRAVN 209
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Fly
Match: Maf1 (gene:FBgn0267861 transcript:FBtr0113831)

HSP 1 Score: 68.1662 bits (165), Expect = 1.187e-13
Identity = 57/188 (30.32%), Postives = 87/188 (46.28%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSL-CNTFGSDANFPLSHPQ-----CDSYRQRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYV-DLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNNNG----INFLFYNDNLKRLLVFTIKFYN 623
            RIE YSCKM   EK  YK    ++ G D    LS PQ       ++R+  +    E   +++   I+ K   +L + L+  +   YD+S+ +S +F  E S + V+ +    +     D Y  IR  +W  +D  + L  C IYSY  +   DP       G     N+ FYN  LKR++ FT +  N
Sbjct:   28 RIESYSCKMVAAEKVLYKRFTADSHGHDLQ-ALSPPQTLADFSPNFRRNNSQSGDEG--ITLCDTISRKTLFYLIATLNASFEPDYDFSEAKSHEFSKEPSLQWVMNSIHANLSALAGDKYQAIRQPLWSAVDDEVILSECDIYSYNPDLSCDPF---GEPGCLWSFNYFFYNKKLKRIVFFTSRAVN 209
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Fly
Match: Maf1 (gene:FBgn0267861 transcript:FBtr0113829)

HSP 1 Score: 68.1662 bits (165), Expect = 1.187e-13
Identity = 57/188 (30.32%), Postives = 87/188 (46.28%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSL-CNTFGSDANFPLSHPQ-----CDSYRQRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYV-DLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNNNG----INFLFYNDNLKRLLVFTIKFYN 623
            RIE YSCKM   EK  YK    ++ G D    LS PQ       ++R+  +    E   +++   I+ K   +L + L+  +   YD+S+ +S +F  E S + V+ +    +     D Y  IR  +W  +D  + L  C IYSY  +   DP       G     N+ FYN  LKR++ FT +  N
Sbjct:   28 RIESYSCKMVAAEKVLYKRFTADSHGHDLQ-ALSPPQTLADFSPNFRRNNSQSGDEG--ITLCDTISRKTLFYLIATLNASFEPDYDFSEAKSHEFSKEPSLQWVMNSIHANLSALAGDKYQAIRQPLWSAVDDEVILSECDIYSYNPDLSCDPF---GEPGCLWSFNYFFYNKKLKRIVFFTSRAVN 209
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Fly
Match: Maf1 (gene:FBgn0267861 transcript:FBtr0344972)

HSP 1 Score: 68.1662 bits (165), Expect = 1.187e-13
Identity = 57/188 (30.32%), Postives = 87/188 (46.28%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSL-CNTFGSDANFPLSHPQ-----CDSYRQRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYV-DLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNNNG----INFLFYNDNLKRLLVFTIKFYN 623
            RIE YSCKM   EK  YK    ++ G D    LS PQ       ++R+  +    E   +++   I+ K   +L + L+  +   YD+S+ +S +F  E S + V+ +    +     D Y  IR  +W  +D  + L  C IYSY  +   DP       G     N+ FYN  LKR++ FT +  N
Sbjct:   28 RIESYSCKMVAAEKVLYKRFTADSHGHDLQ-ALSPPQTLADFSPNFRRNNSQSGDEG--ITLCDTISRKTLFYLIATLNASFEPDYDFSEAKSHEFSKEPSLQWVMNSIHANLSALAGDKYQAIRQPLWSAVDDEVILSECDIYSYNPDLSCDPF---GEPGCLWSFNYFFYNKKLKRIVFFTSRAVN 209
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Zebrafish
Match: maf1 (MAF1 homolog, negative regulator of RNA polymerase III [Source:NCBI gene;Acc:406750])

HSP 1 Score: 78.5666 bits (192), Expect = 3.301e-17
Identity = 51/176 (28.98%), Postives = 80/176 (45.45%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQCDSYRQRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYV-DLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNN-NGINFLFYNDNLKRLLVFTIK 614
            RIE YSCKM   +K+ +K  C          LS PQ         S  ++     +    + K   +L + L+  +R  YD+S+ +  DF  E S   V    +  +     + Y  ++ ++W  +DK I+L  C IYSY  +   DP   + N    N+ FYN  LKR++ FT +
Sbjct:   28 RIESYSCKMAGDDKQMFKQFCQEGQPHVLEALSPPQSSGISPNKLSHSLDDGEGPLSDKCSRKTLFYLIATLNESFRPDYDFSRTKGHDFSKEPSVNWVFNAVNSSLSAAAGEAYSHLQPQLWEALDKEISLAECDIYSYNPDLDSDPYGEEGNLWSFNYFFYNKRLKRIVFFTCR 203
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Zebrafish
Match: zgc:112356 (zgc:112356 [Source:ZFIN;Acc:ZDB-GENE-050913-28])

HSP 1 Score: 69.3218 bits (168), Expect = 4.354e-14
Identity = 54/186 (29.03%), Postives = 83/186 (44.62%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQC-DSYRQRLTSKPIEIQLL--SVKPIINP-------KIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYV-DLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNN-NGINFLFYNDNLKRLLVFTIK 614
            RIE YSCKM   +K  +K  C             P   ++     +S      LL  S +   NP       K   +L + L+  +R  YD+S   + +F  E S   V  + +  + + V + +  +  E+W  ID+ INL+ C IYSY  +   DP   + +    N+ FYN  LKR+L FT +
Sbjct:   28 RIESYSCKMAGDDKHMFKQFCQE---------GEPHVLEALSPPQSSSAPSPNLLGKSGEDAENPLSDKCCRKTLFYLITTLNESFRPDYDFSAARAHEFSREPSANWVANSVNSSLYSAVGEQFNTLGPELWTAIDQEINLQGCDIYSYNPDLDSDPFGEEGSLWSFNYFFYNKKLKRILFFTCR 204
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Zebrafish
Match: zgc:112356 (zgc:112356 [Source:ZFIN;Acc:ZDB-GENE-050913-28])

HSP 1 Score: 69.3218 bits (168), Expect = 8.230e-14
Identity = 54/186 (29.03%), Postives = 83/186 (44.62%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQC-DSYRQRLTSKPIEIQLL--SVKPIINP-------KIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYV-DLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNN-NGINFLFYNDNLKRLLVFTIK 614
            RIE YSCKM   +K  +K  C             P   ++     +S      LL  S +   NP       K   +L + L+  +R  YD+S   + +F  E S   V  + +  + + V + +  +  E+W  ID+ INL+ C IYSY  +   DP   + +    N+ FYN  LKR+L FT +
Sbjct:   28 RIESYSCKMAGDDKHMFKQFCQE---------GEPHVLEALSPPQSSSAPSPNLLGKSGEDAENPLSDKCCRKTLFYLITTLNESFRPDYDFSAARAHEFSREPSANWVANSVNSSLYSAVGEQFNTLGPELWTAIDQEINLQGCDIYSYNPDLDSDPFGEEGSLWSFNYFFYNKKLKRILFFTCR 204
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Mouse
Match: Maf1 (MAF1 homolog, negative regulator of RNA polymerase III [Source:MGI Symbol;Acc:MGI:1916127])

HSP 1 Score: 70.0922 bits (170), Expect = 2.532e-14
Identity = 50/177 (28.25%), Postives = 78/177 (44.07%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQCDSYR-QRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTD-KIIETYVDLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNN-NGINFLFYNDNLKRLLVFTIK 614
            RIE YSCKM   +K  +K  C          LS PQ       RL+          +    + K   +L + L+  +R  YD+S   S +F  E S   VV   +  +     + +  ++ ++W  +D+ I L  C IYSY  +   DP   D +    N+ FYN  LKR++ F+ +
Sbjct:   28 RIESYSCKMAGDDKHMFKQFCQEGQPHVLEALSPPQTSGLSPSRLSKSQGGEDESPLSDKCSRKTLFYLIATLNESFRPDYDFSTARSHEFSREPSLRWVVNAVNCSLFSAVREDFKALKPQLWNAVDEEICLAECDIYSYNPDLDSDPFGEDGSLWSFNYFFYNKRLKRIVFFSCR 204
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Mouse
Match: Maf1 (MAF1 homolog, negative regulator of RNA polymerase III [Source:MGI Symbol;Acc:MGI:1916127])

HSP 1 Score: 70.0922 bits (170), Expect = 4.963e-14
Identity = 50/177 (28.25%), Postives = 78/177 (44.07%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQCDSYR-QRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTD-KIIETYVDLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNN-NGINFLFYNDNLKRLLVFTIK 614
            RIE YSCKM   +K  +K  C          LS PQ       RL+          +    + K   +L + L+  +R  YD+S   S +F  E S   VV   +  +     + +  ++ ++W  +D+ I L  C IYSY  +   DP   D +    N+ FYN  LKR++ F+ +
Sbjct:   28 RIESYSCKMAGDDKHMFKQFCQEGQPHVLEALSPPQTSGLSPSRLSKSQGGEDESPLSDKCSRKTLFYLIATLNESFRPDYDFSTARSHEFSREPSLRWVVNAVNCSLFSAVREDFKALKPQLWNAVDEEICLAECDIYSYNPDLDSDPFGEDGSLWSFNYFFYNKRLKRIVFFSCR 204
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Mouse
Match: Maf1 (MAF1 homolog, negative regulator of RNA polymerase III [Source:MGI Symbol;Acc:MGI:1916127])

HSP 1 Score: 70.0922 bits (170), Expect = 4.963e-14
Identity = 50/177 (28.25%), Postives = 78/177 (44.07%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQCDSYR-QRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTD-KIIETYVDLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNN-NGINFLFYNDNLKRLLVFTIK 614
            RIE YSCKM   +K  +K  C          LS PQ       RL+          +    + K   +L + L+  +R  YD+S   S +F  E S   VV   +  +     + +  ++ ++W  +D+ I L  C IYSY  +   DP   D +    N+ FYN  LKR++ F+ +
Sbjct:   28 RIESYSCKMAGDDKHMFKQFCQEGQPHVLEALSPPQTSGLSPSRLSKSQGGEDESPLSDKCSRKTLFYLIATLNESFRPDYDFSTARSHEFSREPSLRWVVNAVNCSLFSAVREDFKALKPQLWNAVDEEICLAECDIYSYNPDLDSDPFGEDGSLWSFNYFFYNKRLKRIVFFSCR 204
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Mouse
Match: Maf1 (MAF1 homolog, negative regulator of RNA polymerase III [Source:MGI Symbol;Acc:MGI:1916127])

HSP 1 Score: 70.0922 bits (170), Expect = 4.963e-14
Identity = 50/177 (28.25%), Postives = 78/177 (44.07%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQCDSYR-QRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTD-KIIETYVDLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNN-NGINFLFYNDNLKRLLVFTIK 614
            RIE YSCKM   +K  +K  C          LS PQ       RL+          +    + K   +L + L+  +R  YD+S   S +F  E S   VV   +  +     + +  ++ ++W  +D+ I L  C IYSY  +   DP   D +    N+ FYN  LKR++ F+ +
Sbjct:   28 RIESYSCKMAGDDKHMFKQFCQEGQPHVLEALSPPQTSGLSPSRLSKSQGGEDESPLSDKCSRKTLFYLIATLNESFRPDYDFSTARSHEFSREPSLRWVVNAVNCSLFSAVREDFKALKPQLWNAVDEEICLAECDIYSYNPDLDSDPFGEDGSLWSFNYFFYNKRLKRIVFFSCR 204
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. UniProt/SwissProt
Match: sp|Q7ZWL6|MAF1_XENLA (Repressor of RNA polymerase III transcription MAF1 homolog OS=Xenopus laevis OX=8355 GN=maf1 PE=2 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 3.753e-17
Identity = 53/176 (30.11%), Postives = 79/176 (44.89%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQCDSYRQRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDK-IIETYVDLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNN-NGINFLFYNDNLKRLLVFTIK 614
            RIE YSCKM   +K  +K  C          LS PQ      RL       +   +    + K   +L + L+  +R  YD+S   + +F  E S   VV   +  ++    D +  +R  +W  +D+ INL  C IYSY  +   DP   D N    N+ FYN  LKR++ F+ +
Sbjct:   28 RIESYSCKMAGDDKHMFKQFCQEGQPHVLEALSPPQTGISPSRLGKSQGADEEGPLSDKCSRKTLFYLIATLNESFRPDYDFSAARAHEFSREPSLNWVVNAVNSSLVSALGDDFTSLRPNLWDAVDEEINLSECDIYSYNPDLDCDPFGEDGNLWSFNYFFYNKKLKRIVFFSCR 203
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. UniProt/SwissProt
Match: sp|Q6PGU2|MAF1_DANRE (Repressor of RNA polymerase III transcription MAF1 homolog OS=Danio rerio OX=7955 GN=maf1 PE=2 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 2.492e-16
Identity = 51/176 (28.98%), Postives = 80/176 (45.45%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQCDSYRQRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYV-DLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNN-NGINFLFYNDNLKRLLVFTIK 614
            RIE YSCKM   +K+ +K  C          LS PQ         S  ++     +    + K   +L + L+  +R  YD+S+ +  DF  E S   V    +  +     + Y  ++ ++W  +DK I+L  C IYSY  +   DP   + N    N+ FYN  LKR++ FT +
Sbjct:   28 RIESYSCKMAGDDKQMFKQFCQEGQPHVLEALSPPQSSGISPNKLSHSLDDGEGPLSDKCSRKTLFYLIATLNESFRPDYDFSRTKGHDFSKEPSVNWVFNAVNSSLSAAAGEAYSHLQPQLWEALDKEISLAECDIYSYNPDLDSDPYGEEGNLWSFNYFFYNKRLKRIVFFTCR 203
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. UniProt/SwissProt
Match: sp|Q9H063|MAF1_HUMAN (Repressor of RNA polymerase III transcription MAF1 homolog OS=Homo sapiens OX=9606 GN=MAF1 PE=1 SV=2)

HSP 1 Score: 71.2478 bits (173), Expect = 1.667e-13
Identity = 52/177 (29.38%), Postives = 81/177 (45.76%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQCDSYR-QRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYVDLYYK-IRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNN-NGINFLFYNDNLKRLLVFTIK 614
            RIE YSCKM   +K  +K  C          LS PQ       RL+      +   +    + K   +L + L+  +R  YD+S   S +F  E S   VV   +  + + V   +K ++ ++W  +D+ I L  C IYSY  +   DP   D +    N+ FYN  LKR++ F+ +
Sbjct:   28 RIESYSCKMAGDDKHMFKQFCQEGQPHVLEALSPPQTSGLSPSRLSKSQGGEEEGPLSDKCSRKTLFYLIATLNESFRPDYDFSTARSHEFSREPSLSWVVNAVNCSLFSAVREDFKDLKPQLWNAVDEEICLAECDIYSYNPDLDSDPFGEDGSLWSFNYFFYNKRLKRIVFFSCR 204
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. UniProt/SwissProt
Match: sp|A5D9C6|MAF1_BOVIN (Repressor of RNA polymerase III transcription MAF1 homolog OS=Bos taurus OX=9913 GN=MAF1 PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 2.969e-13
Identity = 50/177 (28.25%), Postives = 78/177 (44.07%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQCDSYR-QRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTD-KIIETYVDLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNN-NGINFLFYNDNLKRLLVFTIK 614
            RIE YSCKM   +K  +K  C          LS PQ       RL+          +    + K   +L + L+  +R  YD+S   S +F  E S   VV   +  +     + +  ++ ++W  +D+ I L  C IYSY  +   DP   D +    N+ FYN  LKR++ F+ +
Sbjct:   28 RIESYSCKMAGDDKHMFKQFCQEGQPHVLEALSPPQTSGLSPSRLSKSQGGEDEGPLSDKCSRKTLFYLIATLNESFRPDYDFSTARSHEFSREPSLSWVVNAVNCSLFSAVREDFKALKPQLWNAVDEEICLAECDIYSYNPDLDSDPFGEDGSLWSFNYFFYNKRLKRIVFFSCR 204
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. UniProt/SwissProt
Match: sp|Q9D0U6|MAF1_MOUSE (Repressor of RNA polymerase III transcription MAF1 homolog OS=Mus musculus OX=10090 GN=Maf1 PE=2 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 3.471e-13
Identity = 50/177 (28.25%), Postives = 78/177 (44.07%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQCDSYR-QRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTD-KIIETYVDLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNN-NGINFLFYNDNLKRLLVFTIK 614
            RIE YSCKM   +K  +K  C          LS PQ       RL+          +    + K   +L + L+  +R  YD+S   S +F  E S   VV   +  +     + +  ++ ++W  +D+ I L  C IYSY  +   DP   D +    N+ FYN  LKR++ F+ +
Sbjct:   28 RIESYSCKMAGDDKHMFKQFCQEGQPHVLEALSPPQTSGLSPSRLSKSQGGEDESPLSDKCSRKTLFYLIATLNESFRPDYDFSTARSHEFSREPSLRWVVNAVNCSLFSAVREDFKALKPQLWNAVDEEICLAECDIYSYNPDLDSDPFGEDGSLWSFNYFFYNKRLKRIVFFSCR 204
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. TrEMBL
Match: G7YGR3 (Repressor of RNA polymerase III transcription MAF1 homolog OS=Clonorchis sinensis OX=79923 GN=CLF_107408 PE=4 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 2.002e-18
Identity = 70/258 (27.13%), Postives = 105/258 (40.70%), Query Frame = 3 
Query:   12 MKLLILPNLNKLQDEVKNTFVRYDGDIRIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQCDSYRQR----LTSKPIEIQLLSV-----KPII---------------------------------NPKIYTFLTSALSL------------CYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYVDLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNNNGINFLFYNDNLKRLLVFTIKFYN 623
            MKLL   +L +L  E+     RY+ D+R+E YSCKM  +EKR +K LC+  GSD     SH    S   R    +T + +  +L  +     +P                                   +P  Y    S LS+            C+   YD+    S +FC E     V     +    YVD Y  I  E+W  I++ I    C IYSY+ + + DP         N+ F+N +L+R+L F+++  N
Sbjct:    1 MKLLDQVHLERLVSELSGASKRYNVDVRLESYSCKMVSEEKRQFKELCSRIGSDKQDTESHRLGSSQTLRGLWEMTGQAVNPELYQMSTAQEQPTTAVAHLPANSGSTTAKKRSRSNTASSSDDGASPSSPTRYKTTASGLSVKDLFCLMSTLNSCFGPEYDFLSARSDEFCLEPELWVVKHYISQFCSVYVDKYEDISPELWKTIEEEIVPSQCLIYSYRPDHLSDPYSSGCLASFNYFFHNKSLRRVLFFSLRVLN 258
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. TrEMBL
Match: T1EYF7 (Repressor of RNA polymerase III transcription MAF1 OS=Helobdella robusta OX=6412 GN=20201607 PE=3 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 4.169e-18
Identity = 69/207 (33.33%), Postives = 105/207 (50.72%), Query Frame = 3 
Query:   12 MKLLILPNLNKLQD--EVKNTFVRYDGDIRIECYSCKMTQKEKRHYKSLCNTFGSDAN--FPLSHPQ---CDSYRQRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTD-KIIETYVDLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNNNGINFLFYNDNLKRLLVFT 608
            MKLL  P L  +    ++     R++G  R+E YSCKM  K+KR +K + N  G   N    LS P      S   R  SK  +++ + +   I+ K   +L S L+  +   YD+S+ +S +F  E S   VV + D +++    D Y ++  E+W  ID+ I L+ C  YSY  +   DP + D+    N+ FYN  LKR+L FT
Sbjct:    1 MKLLENPKLEAMSHKLQIDKPDCRFEG--RLESYSCKMVTKDKRLFKLMSNEMGLSPNDLQALSPPVNMFSSSPTSRFGSKSFDMEPV-LCDAISLKTLFYLISTLNESFHPDYDFSQAKSDEFSKESSLASVVNSVDCRLLTVLGDDYNELSKELWATIDEEICLRECSYYSYNPDLTSDPYE-DSLWFFNYFFYNKKLKRILFFT 203
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. TrEMBL
Match: A0A4S2MIC1 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_001174 PE=4 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 6.292e-18
Identity = 70/258 (27.13%), Postives = 104/258 (40.31%), Query Frame = 3 
Query:   12 MKLLILPNLNKLQDEVKNTFVRYDGDIRIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQCDSYRQR----LTSKPIEIQLLSV-----KPII---------------------------------NPKIYTFLTSALSL------------CYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYVDLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNNNGINFLFYNDNLKRLLVFTIKFYN 623
            MKLL   +L +L  E+     RY+ D+R+E YSCKM  +EKR +K LC+  GSD     SH    S   R    +T + +   L  +     +P                                   +P  Y    S LS+            C+   YD+    S +FC E     V     +    YVD Y  I  E+W  I++ I    C IYSY+ + + DP         N+ F+N +L+R+L F+++  N
Sbjct:    1 MKLLDQVHLERLVSELSGASKRYNVDVRLESYSCKMVSEEKRQFKELCSRIGSDKQDTESHRLGSSQTLRGLWEMTGQAVNPDLYHMSTAQEQPTTAVTHLSANSGSTTAKKRSRSNTASSSDDGASPSSPTRYKTTASGLSVKDLFCLMSTLNSCFGPEYDFLSARSDEFCLEPELWVVKHYISQFCSVYVDKYEDISPELWKTIEEEIVPSQCLIYSYRPDHLSDPYSSGCLASFNYFFHNKSLRRVLFFSLRVLN 258
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. TrEMBL
Match: A0A074ZSJ2 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_03128 PE=4 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 6.693e-18
Identity = 69/258 (26.74%), Postives = 105/258 (40.70%), Query Frame = 3 
Query:   12 MKLLILPNLNKLQDEVKNTFVRYDGDIRIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQCDSYRQR----LTSKPIEIQLLSV-----KPII---------------------------------NPKIYTFLTSALSL------------CYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYVDLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNNNGINFLFYNDNLKRLLVFTIKFYN 623
            MKLL   +L +L  E+     RY+ D+R+E YSCKM  +EKR +K LC+  GSD     SH    S   R    +T + +  ++  +     +P                                   +P  Y    S LS+            C+   YD+    S +FC E     V     +    YVD Y +I  E+W  I+  I    C IYSY+ + + DP         N+ F+N +L+R+L F+++  N
Sbjct:    1 MKLLDQVHLERLVSELSGASKRYNVDVRLESYSCKMVSEEKRQFKELCSRIGSDKQDTESHRLGSSQTLRGLWEMTGQAVNPEVYQMSNAQEQPTTAVAHLPANSGSTTAKKRSRSNTASSSDDGASPSSPTRYKTTASGLSVKDLFCLMSTLNSCFGPEYDFLSARSDEFCLEPELWVVKHYISQFCSVYVDKYEEISPELWKTIEDEIVPSQCLIYSYRPDHLSDPYSSGCLASFNYFFHNKSLRRVLFFSLRVLN 258
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. TrEMBL
Match: A0A0P4WKY9 (Repressor of RNA polymerase III transcription MAF1 OS=Scylla olivacea OX=85551 PE=3 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 1.135e-16
Identity = 60/183 (32.79%), Postives = 88/183 (48.09%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQC-------DSYRQRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDK-IIETYVDLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDN-NNGINFLFYNDNLKRLLVFTIK 614
            R+E YSCKM  +EK  YK   +  G      LS PQ         S+ +   S   E   L+ K  IN K   +L S L+  +   YD+S   S +F  E S E V++T  K ++      +  +  E+W  +D  I L+ C+IYSY  ++  DP   D      N+ FYN  LKR++ FT++
Sbjct:   29 RLESYSCKMVGQEKALYKRFHSFSGLSDMQALSPPQSMMEASSPHSHAKSSQSSGGEDSPLADK--INRKTLFYLISTLNAAFYPDYDFSNASSQEFSREPSVEWVMRTVQKQMMAVARAEFMAVESELWAALDSAICLRECEIYSYNPDQTSDPYSEDGCLWSFNYFFYNSRLKRIVFFTMR 209
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Cavefish
Match: maf1 (repressor of RNA polymerase III transcription MAF1 homolog [Source:NCBI gene;Acc:103046362])

HSP 1 Score: 73.9442 bits (180), Expect = 1.443e-15
Identity = 52/178 (29.21%), Postives = 84/178 (47.19%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQCDSYR-QRLT-SKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYV-DLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNN-NGINFLFYNDNLKRLLVFTIK 614
            RIE YSCKM  ++K+ +K  C          LS PQ       +L+ S+  +     +    + K   +L + L+  +R  YD+S+ +  DF  E S   V    +  +     ++Y  ++ ++W  ID  I+L  C IYSY  +   DP   + N    N+ FYN  LKR++ FT +
Sbjct:   28 RIESYSCKMAGEDKQMFKQFCQEGLPHVLEALSPPQTAGVSPNKLSHSQSGDEGEGPLSDKCSRKTLFYLIATLNESFRPDYDFSRTKGHDFSREPSVNWVFNAVNSSLSAAAGEVYSHLQPQLWEAIDSEISLAECDIYSYNPDLASDPYGEEGNLWSFNYFFYNKRLKRIVFFTCR 205
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Cavefish
Match: zgc:112356 (repressor of RNA polymerase III transcription MAF1 homolog [Source:NCBI gene;Acc:103028421])

HSP 1 Score: 64.6994 bits (156), Expect = 3.604e-12
Identity = 53/186 (28.49%), Postives = 81/186 (43.55%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQC-DSYRQRLTSKPIEIQLL--SVKPIINP-------KIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYV-DLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNN-NGINFLFYNDNLKRLLVFTIK 614
            RIE YSCKM   +K  +K  C             P   ++     +S      LL  S     NP       K   +L + L+  +R  YD+S   + +F  E S   V    +  + + V + +  +  E+W  ID+ INL+ C IYSY  +   DP   + +    N+ FYN  LKR++ FT +
Sbjct:   28 RIESYSCKMAGDDKHMFKQFCQE---------GEPHVLEALSPPQSSSAPSPNLLGKSGDEGENPLSDKCCRKTLFYLITTLNESFRPDYDFSAARAHEFSREPSLNWVANAVNSSLFSAVGEEFNSLGPELWNAIDQEINLQGCDIYSYNPDLDSDPFGEEGSLWSFNYFFYNKKLKRIVFFTCR 204
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Nematostella
Match: EDO41550 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S3U5])

HSP 1 Score: 69.3218 bits (168), Expect = 1.839e-14
Identity = 59/194 (30.41%), Postives = 88/194 (45.36%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSLCNTFGSDAN------------FPLS----HPQCDSY--RQRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDK-IIETYVDLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNNN-GINFLFYNDNLKRLLVFTIK 614
            RIE YSCKM   +K+ YKSL    GS  N             PLS    H     Y  R R  S   +    ++   I+ K    L S L+  +   YD+S  +S +FC E S + VV T +  +     + +  +++ +W  ID+ I L  C I+SY  +   DP   + +    N+ FYN  ++R L FT +
Sbjct:   28 RIESYSCKMAGNDKKLYKSLNE--GSAPNDLQALSPSQTTQMPLSVSPTHNPVVVYPDRVRTLSTSSQDGEATLCDTISRKTLFHLISTLNASFYPDYDFSNAKSDEFCREPSLQVVVDTINGNLAANMGEQFLSLKNALWSAIDEEIQLVDCDIFSYNPDLDSDPYGEEGSMWSFNYFFYNKKMRRTLFFTCR 219
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Medaka
Match: zgc:112356 (MAF1 homolog, negative regulator of RNA polymerase III [Source:NCBI gene;Acc:101157096])

HSP 1 Score: 70.0922 bits (170), Expect = 3.613e-14
Identity = 54/183 (29.51%), Postives = 83/183 (45.36%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQCDSYRQRLTSKPIEIQLLSVKPIINP-------KIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYV-DLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNN-NGINFLFYNDNLKRLLVFTIK 614
            RIE YSCKM   +K  +K  C          LS PQ  S      + P ++   S +   NP       K   +L + L+  +R  YD+S   + +F  E S   V    +  + + V + +  +  E+W  ID+ I L+ C IYSY  +   DP   + +    N+ FYN  LKR++ FT +
Sbjct:   28 RIESYSCKMAGDDKHMFKQFCQEGEPHVLEALSPPQSTS-----ATSPSQLGR-SSEDGENPLSDKCCRKTLFYLITTLNESFRPDYDFSAARAHEFSREPSLNFVANAVNGSLFSVVGEDFNSLGPELWNAIDQEITLQSCDIYSYNPDLDSDPFGEEGSLWSFNYFFYNKKLKRIVFFTCR 204
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Medaka
Match: zgc:112356 (MAF1 homolog, negative regulator of RNA polymerase III [Source:NCBI gene;Acc:101157096])

HSP 1 Score: 70.0922 bits (170), Expect = 6.714e-14
Identity = 54/183 (29.51%), Postives = 83/183 (45.36%), Query Frame = 3 
Query:   93 RIECYSCKMTQKEKRHYKSLCNTFGSDANFPLSHPQCDSYRQRLTSKPIEIQLLSVKPIINP-------KIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYV-DLYYKIRHEMWVEIDKIINLKLCQIYSYKSNKIDDPLQIDNN-NGINFLFYNDNLKRLLVFTIK 614
            RIE YSCKM   +K  +K  C          LS PQ  S      + P ++   S +   NP       K   +L + L+  +R  YD+S   + +F  E S   V    +  + + V + +  +  E+W  ID+ I L+ C IYSY  +   DP   + +    N+ FYN  LKR++ FT +
Sbjct:   28 RIESYSCKMAGDDKHMFKQFCQEGEPHVLEALSPPQSTS-----ATSPSQLGR-SSEDGENPLSDKCCRKTLFYLITTLNESFRPDYDFSAARAHEFSREPSLNFVANAVNGSLFSVVGEDFNSLGPELWNAIDQEITLQSCDIYSYNPDLDSDPFGEEGSLWSFNYFFYNKKLKRIVFFTCR 204
[Close]
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Planmine SMEST
Match: SMESG000006492.1 (SMESG000006492.1)

HSP 1 Score: 268.085 bits (684), Expect = 4.313e-92
Identity = 129/129 (100.00%), Postives = 129/129 (100.00%), Query Frame = 3 
Query:  117 MTQKEKRHYKSLCNTFGSDANFPLSHPQCDSYRQRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYVDLYYKIRHEMWVEIDKIINLKLCQIY 503
            MTQKEKRHYKSLCNTFGSDANFPLSHPQCDSYRQRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYVDLYYKIRHEMWVEIDKIINLKLCQIY
Sbjct:    1 MTQKEKRHYKSLCNTFGSDANFPLSHPQCDSYRQRLTSKPIEIQLLSVKPIINPKIYTFLTSALSLCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYVDLYYKIRHEMWVEIDKIINLKLCQIY 129

HSP 2 Score: 53.1434 bits (126), Expect = 1.137e-8
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 1 
Query:  505 VINQIKLMIPFKSITIMELIFYFTMII 585
            VINQIKLMIPFKSITIMELIFYFTMII
Sbjct:  143 VINQIKLMIPFKSITIMELIFYFTMII 169
The following BLAST results are available for this feature:
BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
MAF13.470e-1429.38MAF1 homolog, negative regulator of RNA polymerase... [more]
MAF13.470e-1429.38MAF1 homolog, negative regulator of RNA polymerase... [more]
MAF11.186e-825.12MAF1 homolog, negative regulator of RNA polymerase... [more]
MAF11.626e-824.64MAF1 homolog, negative regulator of RNA polymerase... [more]
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BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mafr-15.337e-1229.08Repressor of RNA polymerase III transcription MAF1... [more]
mafr-15.337e-1229.08Repressor of RNA polymerase III transcription MAF1... [more]
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BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Maf11.187e-1330.32gene:FBgn0267861 transcript:FBtr0301121[more]
Maf11.187e-1330.32gene:FBgn0267861 transcript:FBtr0113830[more]
Maf11.187e-1330.32gene:FBgn0267861 transcript:FBtr0113831[more]
Maf11.187e-1330.32gene:FBgn0267861 transcript:FBtr0113829[more]
Maf11.187e-1330.32gene:FBgn0267861 transcript:FBtr0344972[more]
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BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
maf13.301e-1728.98MAF1 homolog, negative regulator of RNA polymerase... [more]
zgc:1123564.354e-1429.03zgc:112356 [Source:ZFIN;Acc:ZDB-GENE-050913-28][more]
zgc:1123568.230e-1429.03zgc:112356 [Source:ZFIN;Acc:ZDB-GENE-050913-28][more]
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BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Maf12.532e-1428.25MAF1 homolog, negative regulator of RNA polymerase... [more]
Maf14.963e-1428.25MAF1 homolog, negative regulator of RNA polymerase... [more]
Maf14.963e-1428.25MAF1 homolog, negative regulator of RNA polymerase... [more]
Maf14.963e-1428.25MAF1 homolog, negative regulator of RNA polymerase... [more]
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BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q7ZWL6|MAF1_XENLA3.753e-1730.11Repressor of RNA polymerase III transcription MAF1... [more]
sp|Q6PGU2|MAF1_DANRE2.492e-1628.98Repressor of RNA polymerase III transcription MAF1... [more]
sp|Q9H063|MAF1_HUMAN1.667e-1329.38Repressor of RNA polymerase III transcription MAF1... [more]
sp|A5D9C6|MAF1_BOVIN2.969e-1328.25Repressor of RNA polymerase III transcription MAF1... [more]
sp|Q9D0U6|MAF1_MOUSE3.471e-1328.25Repressor of RNA polymerase III transcription MAF1... [more]
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BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
G7YGR32.002e-1827.13Repressor of RNA polymerase III transcription MAF1... [more]
T1EYF74.169e-1833.33Repressor of RNA polymerase III transcription MAF1... [more]
A0A4S2MIC16.292e-1827.13Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A074ZSJ26.693e-1826.74Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A0P4WKY91.135e-1632.79Repressor of RNA polymerase III transcription MAF1... [more]
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BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
maf11.443e-1529.21repressor of RNA polymerase III transcription MAF1... [more]
zgc:1123563.604e-1228.49repressor of RNA polymerase III transcription MAF1... [more]
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BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO415501.839e-1430.41Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
zgc:1123563.613e-1429.51MAF1 homolog, negative regulator of RNA polymerase... [more]
zgc:1123566.714e-1429.51MAF1 homolog, negative regulator of RNA polymerase... [more]
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BLAST of Repressor of RNA polymerase III transcription MAF1-like protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000006492.14.313e-92100.00SMESG000006492.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024845 ID=SMED30024845|Name=Repressor of RNA polymerase III transcription MAF1-like protein|organism=Schmidtea mediterranea sexual|type=transcript|length=689bp
TTATAGCTTAAATGAAGTTGTTGATATTACCAAATTTAAATAAATTACAA
GATGAAGTTAAAAATACATTCGTTAGATATGATGGCGACATACGAATTGA
GTGCTATTCGTGTAAGATGACTCAGAAGGAAAAGAGACATTATAAAAGCT
TGTGTAATACTTTTGGCAGTGATGCAAATTTTCCTCTTAGCCATCCGCAG
TGTGATAGTTACAGGCAAAGGTTAACGTCTAAGCCAATTGAAATTCAGCT
ATTATCAGTTAAGCCAATTATTAATCCTAAAATATATACTTTTTTGACGA
GTGCATTGTCTCTATGTTATCGAGAAGCTTATGACTACAGCAAGTGTGAA
AGTACAGATTTTTGTTACGAAAGAAGTCCAGAAGAAGTTGTTAAAACTAC
AGATAAAATAATTGAAACATATGTTGACTTATACTATAAAATCAGACATG
AAATGTGGGTCGAAATTGATAAAATTATAAATCTCAAACTATGCCAAATC
TACAGTTATAAATCAAATAAAATTGATGATCCCCTTCAAATCGATAACAA
TAATGGAATTAATTTTTTATTTTACAATGATAATCTAAAACGATTGTTGG
TATTCACCATAAAATTTTACAATTTCAAAAATCAAACAAAAGAAGCAGAA
TATTTATTATAAATACATATTTGTTAAATTTAAAAAAAA
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protein sequence of SMED30024845-orf-1

>SMED30024845-orf-1 ID=SMED30024845-orf-1|Name=SMED30024845-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=217bp
MKLLILPNLNKLQDEVKNTFVRYDGDIRIECYSCKMTQKEKRHYKSLCNT
FGSDANFPLSHPQCDSYRQRLTSKPIEIQLLSVKPIINPKIYTFLTSALS
LCYREAYDYSKCESTDFCYERSPEEVVKTTDKIIETYVDLYYKIRHEMWV
EIDKIINLKLCQIYSYKSNKIDDPLQIDNNNGINFLFYNDNLKRLLVFTI
KFYNFKNQTKEAEYLL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
GO:0005634nucleus
Vocabulary: INTERPRO
TermDefinition
IPR038564Maf1_sf
IPR015257Maf1
IPR001024PLAT/LH2_dom
Vocabulary: biological process
TermDefinition
GO:0016480negative regulation of transcription from RNA polymerase III promoter
GO:0006351transcription, DNA-templated
GO:0006355regulation of transcription, DNA-templated
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015257Repressor of RNA polymerase III transcription Maf1PFAMPF09174Maf1coord: 28..198
e-value: 2.4E-29
score: 102.7
IPR015257Repressor of RNA polymerase III transcription Maf1PANTHERPTHR22504REPRESSOR OF RNA POLYMERASE III TRANSCRIPTION MAF1coord: 1..204
IPR038564Repressor of RNA polymerase III transcription Maf1 superfamilyGENE3DG3DSA:3.40.1000.50coord: 63..201
e-value: 5.5E-19
score: 70.5