SMED30024777

Overview
NameSMED30024777
Smed IDSMED30024777
Length (bp)5046
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30024777 (SMED30024777) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30024777 (SMED30024777) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30024777 (SMED30024777) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30024777 (SMED30024777) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30024777

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30024777h1SMcG0015041 SmedASXL_010850SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30024777h1SMcG0015041 SmedASXL_010850SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30024777h1SMcG0015041 Contig33099uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30024777h1SMcG0015041 Contig33099newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
testisSMED30024777h1SMcG0010253 Contig4813uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testisSMED30024777h1SMcG0010253 Contig4813newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testisSMED30024777h1SMcG0015041 Contig4813uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
testisSMED30024777h1SMcG0015041 Contig4813newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30024777 vs. Planmine SMEST
Match: SMESG000011608.1 (SMESG000011608.1)

HSP 1 Score: 640.188 bits (1650), Expect = 0.000e+0
Identity = 339/339 (100.00%), Postives = 339/339 (100.00%), Query Frame = 3
Query:   81 MKLALTMDADKCDLSNLLKRLKESNEEETKIKNKISRQKEEIVKTEKMMQQFQDCLTEQNELLSRLCNNQENAINTTNQIIQAIKQLDNENEEVSTYEDLKNSINSVHSETLIVKNEDKMNLSIIINETDFPVNEIQILPKNEQEIIEPKKRKLNESINGESENSLFVKDKKVESGPFGVCEHRDSKIAELNAEKLKYIQRAKTLEYQCWALESTWFKPEQMRKAQESIQAQRAHIERDEIEIRNLKIQLKGMHIELMKAKNDHHITSSALMSLPYASTELFPPVTYPVIPIYSESLPEEXXXXXXXXXXXXXXXXXXXQEALMMQQQIKSLESALPKL 1097
            MKLALTMDADKCDLSNLLKRLKESNEEETKIKNKISRQKEEIVKTEKMMQQFQDCLTEQNELLSRLCNNQENAINTTNQIIQAIKQLDNENEEVSTYEDLKNSINSVHSETLIVKNEDKMNLSIIINETDFPVNEIQILPKNEQEIIEPKKRKLNESINGESENSLFVKDKKVESGPFGVCEHRDSKIAELNAEKLKYIQRAKTLEYQCWALESTWFKPEQMRKAQESIQAQRAHIERDEIEIRNLKIQLKGMHIELMKAKNDHHITSSALMSLPYASTELFPPVTYPVIPIYSESLPEEKRKSKHRLSRNRRRRIKRRQEALMMQQQIKSLESALPKL
Sbjct:    1 MKLALTMDADKCDLSNLLKRLKESNEEETKIKNKISRQKEEIVKTEKMMQQFQDCLTEQNELLSRLCNNQENAINTTNQIIQAIKQLDNENEEVSTYEDLKNSINSVHSETLIVKNEDKMNLSIIINETDFPVNEIQILPKNEQEIIEPKKRKLNESINGESENSLFVKDKKVESGPFGVCEHRDSKIAELNAEKLKYIQRAKTLEYQCWALESTWFKPEQMRKAQESIQAQRAHIERDEIEIRNLKIQLKGMHIELMKAKNDHHITSSALMSLPYASTELFPPVTYPVIPIYSESLPEEKRKSKHRLSRNRRRRIKRRQEALMMQQQIKSLESALPKL 339          
BLAST of SMED30024777 vs. Planmine SMEST
Match: SMESG000011608.1 (SMESG000011608.1)

HSP 1 Score: 628.246 bits (1619), Expect = 0.000e+0
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 3
Query:   99 MDADKCDLSNLLKRLKESNEEETKIKNKISRQKEEIVKTEKMMQQFQDCLTEQNELLSRLCNNQENAINTTNQIIQAIKQLDNENEEVSTYEDLKNSINSVHSETLIVKNEDKMNLSIIINETDFPVNEIQILPKNEQEIIEPKKRKLNESINGESENSLFVKDKKVESGPFGVCEHRDSKIAELNAEKLKYIQRAKTLEYQCWALESTWFKPEQMRKAQESIQAQRAHIERDEIEIRNLKIQLKGMHIELMKAKNDHHITSSALMSLPYASTELFPPVTYPVIPIYSESLPEEXXXXXXXXXXXXXXXXXXXQEALMMQQQIKSLESALPKL 1097
            MDADKCDLSNLLKRLKESNEEETKIKNKISRQKEEIVKTEKMMQQFQDCLTEQNELLSRLCNNQENAINTTNQIIQAIKQLDNENEEVSTYEDLKNSINSVHSETLIVKNEDKMNLSIIINETDFPVNEIQILPKNEQEIIEPKKRKLNESINGESENSLFVKDKKVESGPFGVCEHRDSKIAELNAEKLKYIQRAKTLEYQCWALESTWFKPEQMRKAQESIQAQRAHIERDEIEIRNLKIQLKGMHIELMKAKNDHHITSSALMSLPYASTELFPPVTYPVIPIYSESLPEEKRKSKHRLSRNRRRRIKRRQEALMMQQQIKSLESALPKL
Sbjct:    1 MDADKCDLSNLLKRLKESNEEETKIKNKISRQKEEIVKTEKMMQQFQDCLTEQNELLSRLCNNQENAINTTNQIIQAIKQLDNENEEVSTYEDLKNSINSVHSETLIVKNEDKMNLSIIINETDFPVNEIQILPKNEQEIIEPKKRKLNESINGESENSLFVKDKKVESGPFGVCEHRDSKIAELNAEKLKYIQRAKTLEYQCWALESTWFKPEQMRKAQESIQAQRAHIERDEIEIRNLKIQLKGMHIELMKAKNDHHITSSALMSLPYASTELFPPVTYPVIPIYSESLPEEKRKSKHRLSRNRRRRIKRRQEALMMQQQIKSLESALPKL 333          
BLAST of SMED30024777 vs. Planmine SMEST
Match: SMESG000017444.1 (SMESG000017444.1)

HSP 1 Score: 191.815 bits (486), Expect = 9.030e-49
Identity = 96/132 (72.73%), Postives = 110/132 (83.33%), Query Frame = -3
Query: 2279 MDPCTILNTALKNARKENTDLHRQLLQLTKEIHQIKSTWIEPVKAKVLYQPVAATQRGWAEEKRLEQATKTQIHGLEVAQSAYQEGQAVT*PLLFAPAQLAYRTSTDATKPAVKPPQRRPGKIEKAKRQAMR 2674
            M   T LN ALK+ARKENTDLHRQ+L++T+EI +IKSTWIEP K KVLYQ V A QR WAEEKRL QA +TQI GLEVA +A QEGQ+VT PL+FAPAQLAYR ST ATKPA+K PQRRPGK E+A+R+AMR
Sbjct:    1 MVTATGLNQALKDARKENTDLHRQMLEMTREIQRIKSTWIEPSKVKVLYQRVTAAQRRWAEEKRLVQAMRTQIRGLEVALAACQEGQSVTYPLVFAPAQLAYRDSTCATKPAIKSPQRRPGKFERARRRAMR 132          
BLAST of SMED30024777 vs. Planmine SMEST
Match: SMESG000020037.1 (SMESG000020037.1)

HSP 1 Score: 166.777 bits (421), Expect = 1.993e-47
Identity = 83/130 (63.85%), Postives = 100/130 (76.92%), Query Frame = -3
Query: 2285 MDPCTILNTALKNARKENTDLHRQLLQLTKEIHQIKSTWIEPVKAKVLYQPVAATQRGWAEEKRLEQATKTQIHGLEVAQSAYQEGQAVT*PLLFAPAQLAYRTSTDATKPAVKPPQRRPGKIEKAKRQA 2674
            M P  I +  L+ +R+EN +LH+QL+Q+TKEIHQIK+TW+EP K K LYQ + A QRGWAEEK+L Q  K QI  LEVA SA QEG AVT PL+FAPAQLAYR +T+ TKPA KP  RRPGK E+AKR+A
Sbjct:    1 MGPYKISSRELEKSRRENAELHQQLIQMTKEIHQIKATWVEPGKVKSLYQKMTAAQRGWAEEKQLHQTQKVQIRSLEVALSACQEGNAVTYPLVFAPAQLAYREATETTKPATKPTPRRPGKAERAKRRA 130          
BLAST of SMED30024777 vs. Planmine SMEST
Match: SMESG000013157.1 (SMESG000013157.1)

HSP 1 Score: 102.064 bits (253), Expect = 8.849e-44
Identity = 47/92 (51.09%), Postives = 66/92 (71.74%), Query Frame = -3
Query: 2918 IARLETHQKKTNKEVNRGRQHLNQINKDIAAGLIPSTDWYAPSYGISFELDDPRVNQPAQAMTVSMPKKILPRGIRTETGDRMYEWDHSYTR 3193
            +ARLE HQ +TN++V + RQ+L ++N+DIAAG IPSTD YAP Y +SFELDDPR+ +    M V++P+ ILP+ +R E      + +HSY R
Sbjct:  165 LARLERHQAETNRQVEQARQYLRRVNEDIAAGRIPSTDGYAPQYNVSFELDDPRLQRQMSEMRVAVPRHILPKEMRNENDIAEPQREHSYAR 256          

HSP 2 Score: 96.6709 bits (239), Expect = 8.849e-44
Identity = 42/71 (59.15%), Postives = 56/71 (78.87%), Query Frame = -2
Query: 3195 WMDESRITMRFPSHSAAKCQQAMWDKGYSISPVRKSRFERQDEMEDAERPYIAIKREPGRNPAQEPRGQAE 3407
            W+D SR+  RFP+HSAA+C+QAMWD G+SI PVR++R   Q +  + +  Y+AIKREPGR+PA+E RGQAE
Sbjct:   92 WIDASRVIARFPAHSAARCRQAMWDAGFSIFPVRETRGGTQGDSNEVDLSYVAIKREPGRSPAREMRGQAE 162          
The following BLAST results are available for this feature:
BLAST of SMED30024777 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024777 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024777 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024777 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024777 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024777 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024777 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024777 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024777 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024777 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024777 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024777 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024777 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024777 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000011608.10.000e+0100.00SMESG000011608.1[more]
SMESG000011608.10.000e+0100.00SMESG000011608.1[more]
SMESG000017444.19.030e-4972.73SMESG000017444.1[more]
SMESG000020037.11.993e-4763.85SMESG000020037.1[more]
SMESG000013157.18.849e-4451.09SMESG000013157.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024777 ID=SMED30024777|Name=SMED30024777|organism=Schmidtea mediterranea sexual|type=transcript|length=5046bp
TCTCACTTGCAAAAAAATAAAATTCGTCATATTATTTTATAATTATTTTT
TCATATTTATTTTTAAATTAATTTTAATCAATGAAGCTAGCTTTGACAAT
GGATGCTGATAAATGTGACTTAAGTAATCTTTTAAAACGTTTAAAAGAAT
CTAACGAAGAAGAAACAAAAATTAAAAACAAAATTTCTAGACAAAAGGAA
GAAATTGTCAAAACTGAAAAAATGATGCAGCAGTTCCAAGATTGTCTTAC
TGAACAGAATGAACTCCTTTCTAGATTGTGCAACAATCAAGAAAATGCAA
TTAATACTACAAATCAAATTATTCAAGCGATAAAACAGTTAGATAATGAA
AATGAAGAGGTTTCGACTTATGAAGATCTTAAAAATTCCATAAATTCTGT
TCATAGTGAAACCTTAATTGTCAAAAACGAAGACAAAATGAATCTATCCA
TTATCATAAATGAAACAGATTTTCCAGTAAATGAAATACAAATTCTTCCT
AAAAATGAACAAGAAATCATTGAACCGAAAAAAAGAAAACTAAACGAATC
GATAAATGGGGAATCTGAAAACAGTTTATTTGTAAAGGATAAAAAAGTAG
AGAGTGGACCATTTGGTGTGTGTGAGCACCGTGACAGTAAGATCGCTGAA
CTGAATGCTGAGAAGCTTAAATACATTCAGCGTGCTAAGACACTTGAGTA
TCAGTGTTGGGCCCTAGAATCAACTTGGTTCAAACCCGAGCAAATGCGCA
AAGCACAAGAATCGATACAGGCTCAACGAGCACACATTGAAAGAGATGAA
ATCGAAATACGCAACTTAAAAATCCAGCTTAAAGGAATGCATATTGAGCT
GATGAAAGCTAAGAATGATCATCATATCACTTCATCAGCCCTCATGTCAC
TTCCTTACGCTAGCACGGAGTTATTTCCGCCTGTTACTTATCCAGTTATT
CCGATTTACTCTGAATCTTTACCCGAGGAAAAGCGAAAATCTAAGCATAG
GCTAAGCAGAAATAGACGGAGACGAATAAAAAGACGACAAGAAGCTTTAA
TGATGCAGCAGCAGATAAAATCGCTCGAGAGCGCCTTACCGAAACTATAG
CTGGCTTATGTTTTAGGAAATTTTTTCCTTATATAAGGACTTTGTGTTTG
TATTTTTACATAAGAATTTTGTAGCATCGTCTTAATTATTGATTTTTTAT
TGTTGTTTACAACTCATGCACCTCTATGTATAATTATTTGCTGCGTGTTA
ATTGTAAGGTTAGAATTATTGTTTTTGGTAAATTTTATTTTGTATACACT
AAAGTGGCCAGACATTTTTTGAAATGCTGAAAATATTCAAATCAAAATTT
TCGTACGTCAATTAAATGAATTATTTACTTTAAAAATTGAAAATAAAATT
TATAGTAAATTAATTAAGCAAAAGATTTTTTTGTATTACAAATGATTTGT
TGGTACTTAAAAATAAAAGCATTATCTAATATTAAGTGGAGGAAACAGAA
ATTTTGTGAAAATGTTTGGTGAGGTCTCAACTTGCACTAGTGTTTCTTAA
ATAAATCAAAAGACAAAGTCGATATATAAATGATTTATTAATAAATAAAA
TTTACCAAATCAATTTTGAAAATTTTCAAATTTAACTTTTCGATCACACT
TTATTCAATTATCAATACAAATGAAATTTGGGTGCTTGTTATGTAATTGT
TAAATTGTATTTCGGTGCGTGTTATATACGTGAATGCAAAATATCGTGAT
GAACTAAATCTGTTCTATTTTAAGATCTTCCAAAGATTACCTTCTTCAGA
TCGCTTCTTTTAGTAGTGAGATTTTTGTGGGCGCTTAGACTGAAAAATAT
CTCAGTATTTATACTAAAATATTTTGCCTGTTGTGGGGTTTTGGGTAGAG
CGCTGTTAATTAACCTTGACCCCATATGAAAATAAATGTCTCTCTCCCAA
TGGTTACCTGTTGCCAAGCAACGTTGGTAATGCAACCACTGGTTATGGAA
TCGCCAAAGGTGAGCGCCATATAGGAACTGCTAAGGCCCGATCCCATAGC
GGGTTCAACGCACTACGATAGAAGGTATGTGTGCGATTTCTATTTCTGGT
CCCTTTCGTTTTAAGAATTCTCTCATTCGATCAGTTATCTCTTTCTCAAC
ACCCGACTGAATCTTTATTATCTTGCGTGAATTATTCCAGGTCATAGTCA
ACTAATTTAACGAATACGAAGGCAATCAACTAAAATATTTATTTATTATG
ACATTTCTCATTATATTCGTTCCGCAAGTCTCATCGCCTGTCGTTTCGCT
TTCTCGATCTTTCCCGGACGGCGTTGAGGTGGTTTCACCGCGGGTTTGGT
TGCGTCTGTTGAGGTTCGGTACGCCAACTGTGCAGGTGCAAATAACAGGG
GTTATGTGACAGCTTGGCCCTCCTGGTATGCTGATTGCGCCACCTCCAGG
CCGTGTATTTGCGTCTTCGTGGCCTGCTCCAATCGTTTTTCTTCAGCCCA
CCCGCGTTGGGTGGCCGCTACTGGCTGGTATAGAACTTTCGCTTTGACTG
GTTCTATCCATGTTGACTTTATCTGATGAATTTCCTTTGTCAATTGTAGT
AACTGTCTATGAAGATCGGTATTCTCCTTGCGTGCGTTTTTGAGAGCCGT
ATTTAGTATCGTGCATGGGTCCATCTATACAACAAAAATGGGTCATTAAT
AGCTTATTTCGTTCATTATACAATAAATAACATAATAACTTCAAAGGGTA
TTACGTCTAAGCTAAGGTCAGGTAATGGTTTACTTTCAGGGCGAAATATT
TCTTACACTAATTATAAATATAAGGAATGAACAATAAAATCAGAAATATA
TATTTCACAAAATTCAGTTGCGACGTGAGCTTGAATTATCTTTCCTTGGG
TTTCGTCTTGATGTTCTGCGCGTGTAGGAGTGATCCCACTCATACATCCG
ATCTCCAGTCTCCGTCCTTATTCCTCTTGGGAGTATTTTCTTCGGCATCG
ACACGGTCATGGCCTGTGCTGGCTGATTTACCCTGGGGTCATCGAGCTCG
AATGAGATGCCATATGAAGGGGCATACCAATCCGTGCTCGGGATCAAGCC
AGCCGCAATATCCTTGTTTATTTGATTGAGATGTTGGCGACCCCGATTTA
CTTCCTTGTTGGTTTTCTTTTGGTGAGTTTCGAGTCGAGCTATCTTCTGC
CTGTCCTCGAGGTTCTTGAGCCGGATTTCGCCCAGGCTCCCGTTTGATGG
CTATATATGGCCGTTCGGCGTCCTCCATTTCATCCTGCCTCTCGAATCTG
GATTTTCGCACCGGGGAGATAGAATACCCCTTATCCCACATCGCCTGTTG
ACATTTTGCGGCGGAGTGGGATGGGAATCTCATCGTGATACGAGATTCGT
CCATCCAAAAACCTGTGAATACGAATTTATTAAATAGTTTAATAAACTGA
ATCGTTCATGTGGTAATTAACATGTAAAACAAATAATTTATTTGTAGCAT
TTATAGTGAACTGATCGGGTTGATATTCATTAAAAAGAAAAGAATTAATC
AAATTAATAATAAATCGTTCGCGTAGAGTTATTAAACCGTGCATCAACCT
ATAGGTGAATTAAGTTTTCTTAACGGTATTTATCAAGTCAATAGTCTAAG
AGGTATTTATGACGGGGAGACTCGTATGCCTTCTAGCTCATAATTCATTG
GGTAGTTCCTGATTTCATCTCGTGACCTTCATTCGTCGTAAATGTCCGAA
TCGCGATTCATAAATTAACTGTTCGATTTCGTTGGTTGAGCTTAGTGTTT
TTAGCGGCAGGTTCAGCAGAATAAATTTTTTATTGTTCTACGGAATTCTC
CAGACATGAAACTGAGTCTCGACGCTCTTCGCTTACACTTGGGCATATAG
TGCATTCACCTACTGATCTAATGTTTCTCTGACAAACGTTTGGCATCGGT
CATGTTGTTAGACGTGATATTAATTTTATAGCTTTCGCCCTGGACATTTG
GCTAGATACTTGCTCGAGCGTGAAGAAAACAGTTAACATGTTTTGTAAGT
GAATCCTGGGGCTGGCGAGAGTTTAAAGGGAAGACTTAGGTGGCTTTCAT
TTGACTGCATAGAAAATTATCGTAATGAGATTCTGACGCTTGCATTTATT
TAATAATAATTATAATAAACTATCTTTGGTCCTTCTGGTTGTATAGGTTA
TTTTTCGTCGGCAGATTTAGATAGTATTTTCTCTCTCGGATAAGGCGAAT
TGGTGCATGAACATTTTATGAATTAAAATGAGTCCATTAAAATAAATTCA
TACTTCCTTGAGCATCGTTTCTTCGCTATAGCATAATTTCGTCCGTGTTC
GGGTATCGCGAGTGGTGGCCTCTGGATCGTAGTTCGTTGGGTCGGTTGTT
TTTCATACTGTTTACGACAAAAAATGTGCCCTAATCGTCTGATCGCGTCT
TTTATACTTTATTCTGTTGAATGTGCCTGTCGTCTACTTTTCCTAACCAC
TGTCTGTCGGTGTGTGCCTCAATTCTCTCAATTTCCGTGCAACTCGCCTG
TTGGTGTGTGTCGACAGATGTCGACTGCCACGTCGTTATATTCCTTCTCC
GCTTGTCAGCAACGGGCTGTGTCTTGCCACTTCAATAAACCATATGTTGC
TGCTTTATTGTTAATTGTCAAAAATTATAATTTTCATTAGCGACGAAATT
TTGATTCAACGGGTTGAGTTGTGGGCCTGGTGAGCGTATCCACTTCTACT
ATCTTCCACGGATGTCAATCTCCTACTACTCGTATTTTGGATATTGGTAT
ATATCTCGCCTCTTTCTCAATGGGTTTCATCTAGGATTTATGTGATTAAT
GCAAGAATTCGTGGTTGTTAAAGGCCTATTAACTGATGATATTCTCCACG
ATCTACAATCCTATTTTCTCGAGCATCGTTTTGATTTTATGTGAAATCGA
TTGATTATTTATGTGATGATCCTAGTTATGCATAAGTATACCACATGGGA
CTATCAATGATACTTTTGTTAATTTTTTCGCAAGTTGTTAGGGCGT
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protein sequence of SMED30024777-orf-1

>SMED30024777-orf-1 ID=SMED30024777-orf-1|Name=SMED30024777-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=122bp
MDESRITMRFPSHSAAKCQQAMWDKGYSISPVRKSRFERQDEMEDAERPY
IAIKREPGRNPAQEPRGQAEDSSTRNSPKENQQGSKSGSPTSQSNKQGYC
GWLDPEHGLVCPFIWHLIRAR*
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protein sequence of SMED30024777-orf-2

>SMED30024777-orf-2 ID=SMED30024777-orf-2|Name=SMED30024777-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=340bp
MKLALTMDADKCDLSNLLKRLKESNEEETKIKNKISRQKEEIVKTEKMMQ
QFQDCLTEQNELLSRLCNNQENAINTTNQIIQAIKQLDNENEEVSTYEDL
KNSINSVHSETLIVKNEDKMNLSIIINETDFPVNEIQILPKNEQEIIEPK
KRKLNESINGESENSLFVKDKKVESGPFGVCEHRDSKIAELNAEKLKYIQ
RAKTLEYQCWALESTWFKPEQMRKAQESIQAQRAHIERDEIEIRNLKIQL
KGMHIELMKAKNDHHITSSALMSLPYASTELFPPVTYPVIPIYSESLPEE
KRKSKHRLSRNRRRRIKRRQEALMMQQQIKSLESALPKL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR027356NPH3_dom