SMED30024770

Overview
NameSMED30024770
Smed IDSMED30024770
Length (bp)2719
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30024770 (SMED30024770) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30024770 (SMED30024770) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30024770 (SMED30024770) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30024770 (SMED30024770) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30024770

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30024770h1SMcG0003202 Contig1465GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30024770h1SMcG0018703 Contig1465GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30024770h1SMcG0003202 dd_Smed_v4_7894_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30024770h1SMcG0003202 dd_Smed_v4_7894_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30024770h1SMcG0003202 dd_Smed_v4_7894_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30024770h1SMcG0003202 Contig54201uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30024770h1SMcG0003202 Contig54201newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
epidermisSMED30024770h1SMcG0003202 dd_Smed_v4_7894_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30024770 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.005416:1..12442 -11034
Homology
BLAST of SMED30024770 vs. TrEMBL
Match: A0A4S2LG83 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008891 PE=4 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 7.595e-11
Identity = 96/442 (21.72%), Postives = 182/442 (41.18%), Query Frame = 3
Query:   93 FILYISQKFSLNLSKTI------LLCYNEKFRIACENPNDYLIIKNSTFGCLKSFKPNSIQFPWCNNSNLCSFYHNCKISLLNRIAQLCSGKKSCNLNVVFSKYIPKDLFPCKELFTQNQLL---SLYIDYECVAKELFISVADLKNV--QLVGGYITSVAYIHQKTSDSYDFKLLAAS---YAEKNLLPIKVIFRPITLLGKQEHHP-------IVFQFQILQNTLSYPSQNNAILSNSTNTCQ---------MGKLYSQDYYAFN----SDHY--------------LLGNETNSRHSIIACQYSPDNVGRVY-EMENATGVFITANVNF-DPRINRDEGLLIKYIAIFC-PLLNITNAKVSYDNKISRSKSLSVNVFATVQCDSGYFFLSSNRSSKVTSM-----------------ILSCDHNKAQWDNQPIIPDRCV 1214
             +L +S  F + L  T       L CY E+ +IAC+  +  +++ N+ FG  K      I     N S +     +C I +   +++LCSG  SC  NV +S  +P D    +E   +       SL+++Y CV+  L   +    +V  + VGGY+ ++ Y   +   + ++++  A       +  LP +     +     Q H P       + F  +I   +L+ P+ ++    N     +         +G+   QDY   +    S HY              L  + T S   +   + +   +GR +  + +A G+   +N+N  D ++   +G+LI+YIA+FC P+ N T   +     ++   S     +A + C S   +L   R ++  ++                   +C+H+   W ++  IP+ C+
Sbjct:    6 LLLLVSTSFIVTLVHTAVSTDWKLTCYGEQLQIACDL-SKIVVLHNAQFGFFKQI----IGRKNLNVSCIAQQRGDCWIDVTPSMSRLCSGYSSCTTNVQYSLELPLDGI-ARECSIETAGFSKPSLFVEYSCVSTTLLTRLTSNSHVDSEQVGGYLATLDYAETRNGGT-EWRMHQADAICRVGEEQLPYRFY---LPAPNTQPHQPSFGLEEMVAFVLRIKDMSLTQPTTHSVTAENGKTKLRLATDQECSNLGQPCQQDYLEISAKLPSRHYSDASFDVIPVTRLCLTESATQSETPVKDSEAT--MIGRTFGPLPSAVGLQFRSNLNLRDSQVIPGKGILIEYIALFCTPIRNPTGYGIVDYRFLTLPHSSHTVAYAEIFCPSDR-WLEPQRPTETEAIHRNQTGIPWWLERIQHTTRACNHHSRSWSDR--IPEACL 432          
BLAST of SMED30024770 vs. Planmine SMEST
Match: SMESG000035481.1 (SMESG000035481.1)

HSP 1 Score: 1645.56 bits (4260), Expect = 0.000e+0
Identity = 831/834 (99.64%), Postives = 831/834 (99.64%), Query Frame = 3
Query:   57 MFQYFINLRFLFFILYISQKFSLNLSKTILLCYNEKFRIACENPNDYLIIKNSTFGCLKSFKPNSIQFPWCNNSNLCSFYHNCKISLLNRIAQLCSGKKSCNLNVVFSKYIPKDLFPCKELFTQNQLLSLYIDYECVAKELFISVADLKNVQLVGGYITSVAYIHQKTSDSYDFKLLAASYAEKNLLPIKVIFRPITLLGKQEHHPIVFQFQILQNTLSYPSQNNAILSNSTNTCQMGKLYSQDYYAFNSDHYLLGNETNSRHSIIACQYSPDNVGRVYEMENATGVFITANVNFDPRINRDEGLLIKYIAIFCPLLNITNAKVSYDNKISRSKSLSVNVFATVQCDSGYFFLSSNRSSKVTSMILSCDHNKAQWDNQPIIPDRCVNHIPVMPTSSIVTVINATMSSWLEVSSESAQRTHTVIYVNGSYIAMSTDGQMSIIIKSTFGIVSLFFIVFCAAXXXXXXXXXXXXCLSKNCIFLSKLFSKQEETIHANSNNTFGNRFEGFVQYRPIGTPISGSLGFIRHSLSESYRQSNRTLITRADSRPTMTSRDSFPTSNNVSAFDVHNVQHNDELPTKVPKQSPIMKILRSASTLKNHSLGVFRPMSLSRQSFNDRCSNFYDGRSSNSQIALLKSRRSNVASEFMDDPSIMWTSDISPSKTMPKSNMPNKRXXXXXXXXXXXXXXRYIPKIYSPTVDTYLEPTLEVSNHLEDYSQKLSSRNEKNKNEDYMIYDHSRVSSNNIDFTCPSSITGSCSDTNNNENEENKRFSMISNNYYGIPSSNMNPNGLRSRVNDPITERIKNKSTESVYESIDEDPICQNTLERRNLALPPPPNE 2558
            MFQYFINLRFLFFILYISQKFSLNLSKTILLCYNEKFRI CENPNDYLIIKNSTFGCLKSFKPNSIQFPWCNNSNLCSFYHNCKISLLNRIAQLCSGKKSCNLNVVFSKYIPKDLFPCKELFTQNQLLSLYIDYECVAKELFISVADLKNVQLVGGYITSVAYIHQKTSDSYDFKLLAASYAEKNLLPIKVIFRPITLLGKQEHHPIVFQFQILQNTLSYPSQNNAILSNSTNTCQMGKLYSQDYYAFNSDHYLLGNETNSRHSIIACQYSPDNVGRVYEMENATGVFITANVNFDPRINRDEGLLIKYIAIFCPLLNITNAKVSYDNKISRSKSLSVNVFATVQCDSGYFFLSSNRSSKVTSMILSCDHNKAQWDNQPIIPDRCVNHIPVMPTSSIVTVINATMSSWLEVSSESAQRTHTVIYVNGSYIAMSTDGQMSIIIKSTFGIVSLFFIVFCAAIYIYLKNKNKIKCLSKNCIFLSKLFSKQEETIHANSNNTFGNRFEGFVQYRPIGTPISGSLGFIRHSLSESYRQSNRTLITRADSRPTMTSRDSF TSNNVSAFDVHNVQHNDELPTKVPKQSPIMKILRSASTLKNHSLGVFRPMSLSRQSFNDRCSNFYDGRSSNSQIALLKSRRSNVASEFMDDPSIMWTSDISPSKTMPKSNMPNKRDSEFSNNSSSDLSLRYIPKIYSPTVDTYLEPTLEVSNHLEDYSQKLS RNEKNKNEDYMIYDHSRVSSNNIDFTCPSSITGSCSDTNNNENEENKRFSMISNNYYGIPSSNMNPNGLRSRVNDPITERIKNKSTESVYESIDEDPICQNTLERRNLALPPPPNE
Sbjct:    1 MFQYFINLRFLFFILYISQKFSLNLSKTILLCYNEKFRIVCENPNDYLIIKNSTFGCLKSFKPNSIQFPWCNNSNLCSFYHNCKISLLNRIAQLCSGKKSCNLNVVFSKYIPKDLFPCKELFTQNQLLSLYIDYECVAKELFISVADLKNVQLVGGYITSVAYIHQKTSDSYDFKLLAASYAEKNLLPIKVIFRPITLLGKQEHHPIVFQFQILQNTLSYPSQNNAILSNSTNTCQMGKLYSQDYYAFNSDHYLLGNETNSRHSIIACQYSPDNVGRVYEMENATGVFITANVNFDPRINRDEGLLIKYIAIFCPLLNITNAKVSYDNKISRSKSLSVNVFATVQCDSGYFFLSSNRSSKVTSMILSCDHNKAQWDNQPIIPDRCVNHIPVMPTSSIVTVINATMSSWLEVSSESAQRTHTVIYVNGSYIAMSTDGQMSIIIKSTFGIVSLFFIVFCAAIYIYLKNKNKIKCLSKNCIFLSKLFSKQEETIHANSNNTFGNRFEGFVQYRPIGTPISGSLGFIRHSLSESYRQSNRTLITRADSRPTMTSRDSFQTSNNVSAFDVHNVQHNDELPTKVPKQSPIMKILRSASTLKNHSLGVFRPMSLSRQSFNDRCSNFYDGRSSNSQIALLKSRRSNVASEFMDDPSIMWTSDISPSKTMPKSNMPNKRDSEFSNNSSSDLSLRYIPKIYSPTVDTYLEPTLEVSNHLEDYSQKLSPRNEKNKNEDYMIYDHSRVSSNNIDFTCPSSITGSCSDTNNNENEENKRFSMISNNYYGIPSSNMNPNGLRSRVNDPITERIKNKSTESVYESIDEDPICQNTLERRNLALPPPPNE 834          
BLAST of SMED30024770 vs. Planmine SMEST
Match: SMESG000035481.1 (SMESG000035481.1)

HSP 1 Score: 1637.47 bits (4239), Expect = 0.000e+0
Identity = 830/836 (99.28%), Postives = 831/836 (99.40%), Query Frame = 3
Query:   57 MFQYFINLRFLFFILYISQKFSLNLSKTILLCYNEKFRIACENPNDYLIIKNSTFGCLKSFKPNSIQFPWCNNSNLCSFYHNCKISLLNRIAQLCSGKKSCNLNVVFSKYIPKDLFPCKELFTQNQLLSLYIDYECVAKELFISVADLKNVQLVGGYITSVAYIHQKTSDSYDFKLLAASYAEKNLLPIKVIFRPITLLGKQEHHPIVFQFQILQNTLSYPSQNNAILSNSTNTCQMGKLYSQDYYAFNSDHYLLGNETNSRHSIIACQYSPDNVGRVYEMENATGVFITANVNFDPRINRDEGLLIKYIAIFCPLLNITNAKVSYDNKISRSKSLSVNVFATVQCDSGYFFLSSNRSSKVTSMILSCDHNKAQWDNQPIIPDRCVN--HIPVMPTSSIVTVINATMSSWLEVSSESAQRTHTVIYVNGSYIAMSTDGQMSIIIKSTFGIVSLFFIVFCAAXXXXXXXXXXXXCLSKNCIFLSKLFSKQEETIHANSNNTFGNRFEGFVQYRPIGTPISGSLGFIRHSLSESYRQSNRTLITRADSRPTMTSRDSFPTSNNVSAFDVHNVQHNDELPTKVPKQSPIMKILRSASTLKNHSLGVFRPMSLSRQSFNDRCSNFYDGRSSNSQIALLKSRRSNVASEFMDDPSIMWTSDISPSKTMPKSNMPNKRXXXXXXXXXXXXXXRYIPKIYSPTVDTYLEPTLEVSNHLEDYSQKLSSRNEKNKNEDYMIYDHSRVSSNNIDFTCPSSITGSCSDTNNNENEENKRFSMISNNYYGIPSSNMNPNGLRSRVNDPITERIKNKSTESVYESIDEDPICQNTLERRNLALPPPPNE 2558
            MFQYFINLRFLFFILYISQKFSLNLSKTILLCYNEKFRI CENPNDYLIIKNSTFGCLKSFKPNSIQFPWCNNSNLCSFYHNCKISLLNRIAQLCSGKKSCNLNVVFSKYIPKDLFPCKELFTQNQLLSLYIDYECVAKELFISVADLKNVQLVGGYITSVAYIHQKTSDSYDFKLLAASYAEKNLLPIKVIFRPITLLGKQEHHPIVFQFQILQNTLSYPSQNNAILSNSTNTCQMGKLYSQDYYAFNSDHYLLGNETNSRHSIIACQYSPDNVGRVYEMENATGVFITANVNFDPRINRDEGLLIKYIAIFCPLLNITNAKVSYDNKISRSKSLSVNVFATVQCDSGYFFLSSNRSSKVTSMILSCDHNKAQWDNQPIIPDRCVN  +IPVMPTSSIVTVINATMSSWLEVSSESAQRTHTVIYVNGSYIAMSTDGQMSIIIKSTFGIVSLFFIVFCAAIYIYLKNKNKIKCLSKNCIFLSKLFSKQEETIHANSNNTFGNRFEGFVQYRPIGTPISGSLGFIRHSLSESYRQSNRTLITRADSRPTMTSRDSF TSNNVSAFDVHNVQHNDELPTKVPKQSPIMKILRSASTLKNHSLGVFRPMSLSRQSFNDRCSNFYDGRSSNSQIALLKSRRSNVASEFMDDPSIMWTSDISPSKTMPKSNMPNKRDSEFSNNSSSDLSLRYIPKIYSPTVDTYLEPTLEVSNHLEDYSQKLS RNEKNKNEDYMIYDHSRVSSNNIDFTCPSSITGSCSDTNNNENEENKRFSMISNNYYGIPSSNMNPNGLRSRVNDPITERIKNKSTESVYESIDEDPICQNTLERRNLALPPPPNE
Sbjct:    1 MFQYFINLRFLFFILYISQKFSLNLSKTILLCYNEKFRIVCENPNDYLIIKNSTFGCLKSFKPNSIQFPWCNNSNLCSFYHNCKISLLNRIAQLCSGKKSCNLNVVFSKYIPKDLFPCKELFTQNQLLSLYIDYECVAKELFISVADLKNVQLVGGYITSVAYIHQKTSDSYDFKLLAASYAEKNLLPIKVIFRPITLLGKQEHHPIVFQFQILQNTLSYPSQNNAILSNSTNTCQMGKLYSQDYYAFNSDHYLLGNETNSRHSIIACQYSPDNVGRVYEMENATGVFITANVNFDPRINRDEGLLIKYIAIFCPLLNITNAKVSYDNKISRSKSLSVNVFATVQCDSGYFFLSSNRSSKVTSMILSCDHNKAQWDNQPIIPDRCVNREYIPVMPTSSIVTVINATMSSWLEVSSESAQRTHTVIYVNGSYIAMSTDGQMSIIIKSTFGIVSLFFIVFCAAIYIYLKNKNKIKCLSKNCIFLSKLFSKQEETIHANSNNTFGNRFEGFVQYRPIGTPISGSLGFIRHSLSESYRQSNRTLITRADSRPTMTSRDSFQTSNNVSAFDVHNVQHNDELPTKVPKQSPIMKILRSASTLKNHSLGVFRPMSLSRQSFNDRCSNFYDGRSSNSQIALLKSRRSNVASEFMDDPSIMWTSDISPSKTMPKSNMPNKRDSEFSNNSSSDLSLRYIPKIYSPTVDTYLEPTLEVSNHLEDYSQKLSPRNEKNKNEDYMIYDHSRVSSNNIDFTCPSSITGSCSDTNNNENEENKRFSMISNNYYGIPSSNMNPNGLRSRVNDPITERIKNKSTESVYESIDEDPICQNTLERRNLALPPPPNE 836          
The following BLAST results are available for this feature:
BLAST of SMED30024770 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024770 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024770 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024770 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024770 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024770 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024770 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024770 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
A0A4S2LG837.595e-1121.72Uncharacterized protein OS=Opisthorchis felineus O... [more]
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BLAST of SMED30024770 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024770 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024770 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024770 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024770 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024770 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000035481.10.000e+099.64SMESG000035481.1[more]
SMESG000035481.10.000e+099.28SMESG000035481.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024770 ID=SMED30024770|Name=SMED30024770|organism=Schmidtea mediterranea sexual|type=transcript|length=2719bp
CTTGAAAGATTTTACATTTTAGCACAGTTATTTAAAATTTATAAATTATG
TAACCGATGTTTCAATACTTCATCAATTTAAGATTTCTATTTTTTATTCT
ATACATATCACAAAAATTTAGTCTAAATTTATCAAAAACTATTTTATTAT
GTTATAATGAAAAGTTTCGAATCGCGTGTGAAAATCCAAATGATTATTTG
ATAATAAAAAATTCTACTTTTGGATGCTTAAAATCTTTCAAGCCAAATTC
TATTCAATTTCCCTGGTGCAACAATTCTAATTTGTGCAGTTTTTATCATA
ATTGCAAAATTTCGTTATTAAATAGAATTGCTCAATTATGTTCAGGCAAA
AAGTCATGCAATTTAAATGTTGTGTTTTCTAAATATATCCCTAAAGATTT
ATTTCCGTGTAAAGAATTATTTACACAGAATCAATTATTGAGCCTTTATA
TAGATTATGAATGCGTTGCGAAAGAACTATTTATAAGCGTAGCAGACCTA
AAGAACGTTCAATTGGTTGGAGGTTACATCACATCGGTGGCCTATATTCA
CCAGAAAACCAGTGACAGTTATGATTTTAAATTATTAGCTGCTAGTTATG
CTGAAAAAAATTTACTTCCGATCAAAGTAATATTTCGTCCAATCACATTG
CTTGGAAAGCAAGAACATCATCCAATTGTCTTTCAATTTCAGATTTTGCA
AAATACTCTTTCTTACCCATCTCAGAATAATGCAATTTTATCGAATTCAA
CAAATACTTGCCAAATGGGAAAACTGTATTCCCAAGATTATTATGCATTT
AATTCTGACCACTATTTGCTCGGTAATGAAACCAACAGTCGACACTCCAT
AATTGCCTGTCAATACTCACCTGACAATGTCGGTAGGGTTTATGAAATGG
AAAACGCAACAGGCGTGTTCATAACGGCCAATGTGAATTTTGATCCACGA
ATAAATCGTGATGAAGGGCTTCTGATAAAATACATCGCTATCTTTTGTCC
TTTGTTAAATATCACAAATGCAAAAGTAAGTTACGATAACAAAATCAGCC
GATCAAAAAGTTTGAGTGTAAATGTTTTCGCCACTGTCCAATGTGATTCC
GGGTATTTCTTTCTCTCTTCAAATCGTTCTTCTAAAGTAACTTCAATGAT
TTTGAGCTGCGATCACAACAAAGCCCAATGGGATAATCAACCGATCATTC
CAGATCGATGCGTCAACCACATTCCAGTGATGCCAACTTCGTCGATTGTC
ACAGTAATCAACGCTACGATGTCTAGTTGGTTGGAAGTTTCTTCGGAAAG
TGCTCAAAGAACGCACACTGTGATCTATGTGAATGGATCTTATATTGCTA
TGTCAACGGATGGTCAAATGAGCATAATCATTAAAAGTACATTTGGAATT
GTCAGCTTATTTTTTATCGTTTTTTGTGCTGCCATATATATATACCTCAA
GAATAAAAATAAAATAAAATGTTTATCTAAAAACTGTATATTCTTGTCAA
AGTTATTTAGTAAGCAAGAAGAAACTATTCATGCTAATAGCAATAATACA
TTTGGAAATCGTTTCGAAGGTTTTGTCCAATATCGTCCTATTGGAACTCC
AATTTCCGGATCATTGGGATTCATAAGACATAGTTTATCTGAAAGCTATA
GGCAAAGCAACCGAACGCTAATAACTAGAGCAGACAGTAGACCAACTATG
ACAAGTCGAGATTCTTTTCCAACTTCTAATAATGTTTCCGCATTTGACGT
TCATAATGTACAACACAATGATGAATTGCCAACAAAAGTTCCTAAACAAT
CACCAATTATGAAAATCTTGCGTTCAGCTAGTACTTTAAAAAATCATTCC
CTGGGTGTCTTTAGACCAATGTCTTTATCGCGACAGTCTTTTAACGATCG
CTGTTCTAATTTTTATGACGGACGATCCTCTAATTCGCAAATTGCTCTCT
TAAAAAGTCGTCGGAGTAACGTTGCTTCTGAGTTCATGGATGATCCCTCA
ATCATGTGGACTTCAGATATATCTCCGTCTAAAACTATGCCAAAGTCCAA
TATGCCAAATAAGCGTGATTCTGAATTCAGCAATAATTCTAGCTCGGATC
TTTCACTTAGATATATTCCTAAGATTTATTCACCAACAGTTGACACATAC
TTGGAACCTACGCTTGAAGTTTCAAATCATCTTGAAGATTATTCTCAGAA
ATTATCGTCAAGAAATGAAAAGAATAAAAACGAAGATTATATGATTTATG
ATCATTCTAGAGTTTCTAGCAATAATATTGATTTTACTTGTCCATCATCT
ATAACTGGATCGTGTTCTGATACAAATAACAATGAAAATGAAGAAAACAA
AAGATTTTCAATGATCAGTAATAATTATTATGGTATTCCATCTTCAAATA
TGAATCCAAATGGTTTACGATCGCGCGTTAATGATCCAATAACTGAAAGA
ATTAAAAATAAATCAACGGAATCAGTTTATGAAAGTATCGACGAAGATCC
TATATGTCAGAATACATTAGAAAGGAGAAATCTTGCCTTGCCTCCACCGC
CAAATGAATAAATGATAAAGTATTCCGAAGAAAGTGTTTAGTAAACTTTT
GAAAAATGTTTTTTACAAAATTTTATTTTTTCATTTGTTTCAATTGTGAT
TAAGGTTTTGGAGAACTTTTCAGCTATTTTGTCATTTTCTCTTGATATCT
ATTTAATGCCATTAAATCC
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protein sequence of SMED30024770-orf-1

>SMED30024770-orf-1 ID=SMED30024770-orf-1|Name=SMED30024770-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=835bp
MFQYFINLRFLFFILYISQKFSLNLSKTILLCYNEKFRIACENPNDYLII
KNSTFGCLKSFKPNSIQFPWCNNSNLCSFYHNCKISLLNRIAQLCSGKKS
CNLNVVFSKYIPKDLFPCKELFTQNQLLSLYIDYECVAKELFISVADLKN
VQLVGGYITSVAYIHQKTSDSYDFKLLAASYAEKNLLPIKVIFRPITLLG
KQEHHPIVFQFQILQNTLSYPSQNNAILSNSTNTCQMGKLYSQDYYAFNS
DHYLLGNETNSRHSIIACQYSPDNVGRVYEMENATGVFITANVNFDPRIN
RDEGLLIKYIAIFCPLLNITNAKVSYDNKISRSKSLSVNVFATVQCDSGY
FFLSSNRSSKVTSMILSCDHNKAQWDNQPIIPDRCVNHIPVMPTSSIVTV
INATMSSWLEVSSESAQRTHTVIYVNGSYIAMSTDGQMSIIIKSTFGIVS
LFFIVFCAAIYIYLKNKNKIKCLSKNCIFLSKLFSKQEETIHANSNNTFG
NRFEGFVQYRPIGTPISGSLGFIRHSLSESYRQSNRTLITRADSRPTMTS
RDSFPTSNNVSAFDVHNVQHNDELPTKVPKQSPIMKILRSASTLKNHSLG
VFRPMSLSRQSFNDRCSNFYDGRSSNSQIALLKSRRSNVASEFMDDPSIM
WTSDISPSKTMPKSNMPNKRDSEFSNNSSSDLSLRYIPKIYSPTVDTYLE
PTLEVSNHLEDYSQKLSSRNEKNKNEDYMIYDHSRVSSNNIDFTCPSSIT
GSCSDTNNNENEENKRFSMISNNYYGIPSSNMNPNGLRSRVNDPITERIK
NKSTESVYESIDEDPICQNTLERRNLALPPPPNE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 658..677
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..26
score: 0.53
NoneNo IPR availableTMHMMTMhelixcoord: 445..464