Innexin

Overview
NameInnexin
Smed IDSMED30024751
Length (bp)721
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Innexin (SMED30024751) t-SNE clustered cells

Violin plots show distribution of expression levels for Innexin (SMED30024751) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Innexin (SMED30024751) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Innexin (SMED30024751) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30024751

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30024751SMESG000035987.1 dd_Smed_v4_12498_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30024751SMESG000035987.1 dd_Smed_v4_12498_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
reproductive organSMED30024751SMESG000035987.1 Contig8907newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30024751SMESG000035987.1 Contig8907uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Innexin vs. Ensembl Celegans
Match: inx-1 (Innexin [Source:UniProtKB/TrEMBL;Acc:Q17394])

HSP 1 Score: 70.8626 bits (172), Expect = 4.842e-14
Identity = 36/97 (37.11%), Postives = 53/97 (54.64%), Query Frame = 2 
Query:    2 FLWFWFVFIAIITVHSFIHWTSKSVCRIQRYNFIRKFLVLKDALQEAGDRKKLYAFIDLFLQQDGVFILRLLALNCGDLIAAEITEYLWCRFKECAR 292
            FLWFW+  +A ++  S  HW   S    Q   FIRK+L   D    A DR+ +  F+  FL  DGVF +R+++ + GD++A E+   LW  F +  R
Sbjct:  278 FLWFWYFMVAFVSAVSMFHWIIISFLPGQHMKFIRKYLRATDL---ATDRQSVKKFVHKFLGYDGVFCMRMISAHAGDILATELIVALWHNFNDRVR 371
[Close]
BLAST of Innexin vs. Ensembl Celegans
Match: inx-1 (Innexin [Source:UniProtKB/TrEMBL;Acc:Q17394])

HSP 1 Score: 70.0922 bits (170), Expect = 1.013e-13
Identity = 36/97 (37.11%), Postives = 53/97 (54.64%), Query Frame = 2 
Query:    2 FLWFWFVFIAIITVHSFIHWTSKSVCRIQRYNFIRKFLVLKDALQEAGDRKKLYAFIDLFLQQDGVFILRLLALNCGDLIAAEITEYLWCRFKECAR 292
            FLWFW+  +A ++  S  HW   S    Q   FIRK+L   D    A DR+ +  F+  FL  DGVF +R+++ + GD++A E+   LW  F +  R
Sbjct:  278 FLWFWYFMVAFVSAVSMFHWIIISFLPGQHMKFIRKYLRATDL---ATDRQSVKKFVHKFLGYDGVFCMRMISAHAGDILATELIVALWHNFNDRVR 371
[Close]
BLAST of Innexin vs. Ensembl Celegans
Match: inx-1 (Innexin [Source:UniProtKB/TrEMBL;Acc:Q17394])

HSP 1 Score: 70.0922 bits (170), Expect = 1.013e-13
Identity = 36/97 (37.11%), Postives = 53/97 (54.64%), Query Frame = 2 
Query:    2 FLWFWFVFIAIITVHSFIHWTSKSVCRIQRYNFIRKFLVLKDALQEAGDRKKLYAFIDLFLQQDGVFILRLLALNCGDLIAAEITEYLWCRFKECAR 292
            FLWFW+  +A ++  S  HW   S    Q   FIRK+L   D    A DR+ +  F+  FL  DGVF +R+++ + GD++A E+   LW  F +  R
Sbjct:  278 FLWFWYFMVAFVSAVSMFHWIIISFLPGQHMKFIRKYLRATDL---ATDRQSVKKFVHKFLGYDGVFCMRMISAHAGDILATELIVALWHNFNDRVR 371
[Close]
BLAST of Innexin vs. Ensembl Celegans
Match: unc-7 (Innexin unc-7 [Source:UniProtKB/Swiss-Prot;Acc:Q03412])

HSP 1 Score: 66.6254 bits (161), Expect = 1.887e-12
Identity = 36/99 (36.36%), Postives = 58/99 (58.59%), Query Frame = 2 
Query:    2 FLWFWFVFIAIITVHSFIHWTSKSVCRIQRYNFIRKFL-VLKD-ALQEAGDRKKLYAFIDLFLQQDGVFILRLLALNCGDLIAAEITEYLWCRFKECAR 292
            FLWFWF+   I+TV + ++W        Q  +F+RK+L VL D   +   D   L  F + FL++DGVF+LR+++ + G+L+++E+   LW  F    R
Sbjct:  432 FLWFWFLTCGIVTVCNTMYWILIMFIPSQGMSFVRKYLRVLPDHPAKPIADDVTLRKFTNNFLRKDGVFMLRMISTHAGELMSSELILALWQDFNNVDR 530
[Close]
BLAST of Innexin vs. Ensembl Celegans
Match: unc-7 (Innexin unc-7 [Source:UniProtKB/Swiss-Prot;Acc:Q03412])

HSP 1 Score: 66.2402 bits (160), Expect = 2.012e-12
Identity = 36/99 (36.36%), Postives = 58/99 (58.59%), Query Frame = 2 
Query:    2 FLWFWFVFIAIITVHSFIHWTSKSVCRIQRYNFIRKFL-VLKD-ALQEAGDRKKLYAFIDLFLQQDGVFILRLLALNCGDLIAAEITEYLWCRFKECAR 292
            FLWFWF+   I+TV + ++W        Q  +F+RK+L VL D   +   D   L  F + FL++DGVF+LR+++ + G+L+++E+   LW  F    R
Sbjct:  430 FLWFWFLTCGIVTVCNTMYWILIMFIPSQGMSFVRKYLRVLPDHPAKPIADDVTLRKFTNNFLRKDGVFMLRMISTHAGELMSSELILALWQDFNNVDR 528
[Close]
BLAST of Innexin vs. UniProt/SwissProt
Match: sp|Q03412|UNC7_CAEEL (Innexin unc-7 OS=Caenorhabditis elegans OX=6239 GN=unc-7 PE=1 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 3.092e-11
Identity = 36/99 (36.36%), Postives = 58/99 (58.59%), Query Frame = 2 
Query:    2 FLWFWFVFIAIITVHSFIHWTSKSVCRIQRYNFIRKFL-VLKD-ALQEAGDRKKLYAFIDLFLQQDGVFILRLLALNCGDLIAAEITEYLWCRFKECAR 292
            FLWFWF+   I+TV + ++W        Q  +F+RK+L VL D   +   D   L  F + FL++DGVF+LR+++ + G+L+++E+   LW  F    R
Sbjct:  408 FLWFWFLTCGIVTVCNTMYWILIMFIPSQGMSFVRKYLRVLPDHPAKPIADDVTLRKFTNNFLRKDGVFMLRMISTHAGELMSSELILALWQDFNNVDR 506
[Close]
BLAST of Innexin vs. UniProt/SwissProt
Match: sp|O61715|INX19_CAEEL (Innexin-19 OS=Caenorhabditis elegans OX=6239 GN=inx-19 PE=1 SV=2)

HSP 1 Score: 63.929 bits (154), Expect = 1.604e-10
Identity = 32/113 (28.32%), Postives = 61/113 (53.98%), Query Frame = 2 
Query:    2 FLWFWFVFIAIITVHSFIHWTSKSVCRIQRYNFIRKFLVLKDA--------LQEAGDRKKLY-----------AFIDLFLQQDGVFILRLLALNCGDLIAAEITEYLWCRFKE 283
            FLW W++ +AIIT  SFI+W + S    ++ +++ KF+ + ++         ++    ++LY            F+  FL+ DGV +LR+++ + GD+I  ++   LW  F+E
Sbjct:  304 FLWCWYMILAIITTCSFIYWIANSFIHSEKVDYVMKFIQIAESSEFKKLQKFEKDATVERLYTVIAFAPHLLDTFVSDFLKSDGVLMLRMISNHAGDMIVVQLVRNLWQEFRE 416
[Close]
BLAST of Innexin vs. UniProt/SwissProt
Match: sp|O01393|UNC9_CAEEL (Innexin unc-9 OS=Caenorhabditis elegans OX=6239 GN=unc-9 PE=1 SV=1)

HSP 1 Score: 62.7734 bits (151), Expect = 3.958e-10
Identity = 37/102 (36.27%), Postives = 57/102 (55.88%), Query Frame = 2 
Query:    2 FLWFWFVFIAIITVHSFIHWTSKSVCRIQRYNFIRKFLVLKDALQEAGDRKKLYAFIDLFLQQDGVFILRLLALNCGDLIAAEITEYLW---CRFKECARMD 298
            FLWFW+  +A  T+ S  +W   SV   ++ NF+ K+L   +  +   D + L  F+  FL+QDGVF+LR++A + G+L   E+ + LW   C  KE    D
Sbjct:  285 FLWFWYFLLAGATLCSLFYWIYISVVPSRQLNFVGKYLTGIEGYKMV-DSQSLRRFVFHFLRQDGVFLLRMVATHAGELPCYELAKTLWNNYCDNKEGKMHD 385
[Close]
BLAST of Innexin vs. UniProt/SwissProt
Match: sp|A8WVX4|INX19_CAEBR (Innexin-19 OS=Caenorhabditis briggsae OX=6238 GN=inx-19 PE=3 SV=3)

HSP 1 Score: 62.3882 bits (150), Expect = 5.310e-10
Identity = 30/113 (26.55%), Postives = 62/113 (54.87%), Query Frame = 2 
Query:    2 FLWFWFVFIAIITVHSFIHWTSKSVCRIQRYNFIRKFLVLKDA--------LQEAGDRKKLY-----------AFIDLFLQQDGVFILRLLALNCGDLIAAEITEYLWCRFKE 283
            FLW W++ +AIIT  SFI+W + S    ++ +++ KF+ + ++         ++    ++LY           +F+  FL+ DG+ +LR+++ + GD+I  ++   LW  ++E
Sbjct:  288 FLWCWYMILAIITTCSFIYWIANSFIHSEKVDYVMKFIQIAESSEYKKLQKFEKDATVERLYTVIAFAPHLLDSFVSDFLKSDGILMLRMISNHAGDMIVVQLVRNLWQEYRE 400
[Close]
BLAST of Innexin vs. UniProt/SwissProt
Match: sp|Q22549|INX10_CAEEL (Innexin-10 OS=Caenorhabditis elegans OX=6239 GN=inx-10 PE=3 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 2.536e-9
Identity = 30/95 (31.58%), Postives = 51/95 (53.68%), Query Frame = 2 
Query:    2 FLWFWFVFIAIITVHSFIHWTSKSVCRIQRYNFIRKFLVLKDAL---QEAGDRKKLYAFIDLFLQQDGVFILRLLALNCGDLIAAEITEYLWCRF 277
             LWFW++ + + T  SF +W   S+ R     FI + L + D      E G ++K+  FI  +L+ DGVF++R++ L  G +   ++ + LW  F
Sbjct:  286 LLWFWYLALLVFTFGSFFYWLLVSLWRHLNVRFIIRHLEMSDIAFDSSEDGAQEKVNRFISNYLKSDGVFVIRMMTLQSGVIFGTDLVQELWRNF 380
[Close]
BLAST of Innexin vs. TrEMBL
Match: W6V7B3 (Innexin OS=Echinococcus granulosus OX=6210 GN=inx PE=3 SV=1)

HSP 1 Score: 117.472 bits (293), Expect = 3.678e-27
Identity = 58/134 (43.28%), Postives = 90/134 (67.16%), Query Frame = 2 
Query:    2 FLWFWFVFIAIITVHSFIHWTSKSVCRIQRYNFIRKFLVLKDALQEAGDRKKLYAFIDLFLQQDGVFILRLLALNCGDLIAAEITEYLWCRFKECARMDSGERSKLFYLD-----SGDVYSKARPYRSRKSKDL 388
            FLWFWF+ ++ +++ SF++W+ KS  R  R +FIRKFL L+D+L E G++K+ YAF+D +L+QDGVFILRL+A N G+L+A+EI E LW  FK    + + E+ +   +      SG  +  + P R+ +S +L
Sbjct:  155 FLWFWFILVSAVSISSFLYWSYKSSFRSNRIHFIRKFLKLRDSL-EPGEKKRSYAFVDSYLRQDGVFILRLVAQNAGELVASEIVEKLWQMFKRRQSLPTTEKVRANEVGWKSGVSGHSHIHSPPMRAPRSSEL 287
[Close]
BLAST of Innexin vs. TrEMBL
Match: A0A0R3X3I0 (Innexin OS=Hydatigena taeniaeformis OX=6205 GN=inx PE=3 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 4.613e-27
Identity = 62/150 (41.33%), Postives = 95/150 (63.33%), Query Frame = 2 
Query:    2 FLWFWFVFIAIITVHSFIHWTSKSVCRIQRYNFIRKFLVLKDALQEAGDRKKLYAFIDLFLQQDGVFILRLLALNCGDLIAAEITEYLWCRFKECARMDSGERSKLFYLD-----SGDVYSKARPYRSRKSKDLIEPVQSRRRIPSAPAL 436
            FLWFWF+ ++ +++ SF++W+ KS  R  R +FIRKFL L+D+L E G++K+ YAF+D +L+QDGVFILRL+A N G+L+A+EI E LW  FK    +   E+++   +      SG  +  + P R  +S +L  P        +AP+L
Sbjct:  155 FLWFWFILVSAVSISSFLYWSYKSSFRSNRIHFIRKFLKLRDSL-EPGEKKRSYAFVDSYLRQDGVFILRLVAQNAGELVASEIVEKLWQMFKRRQSIPVTEKARTNEVGWKSGVSGHSHIHSPPMRVPRSSEL-SPTSITSPTVNAPSL 302
[Close]
BLAST of Innexin vs. TrEMBL
Match: A0A158R819 (Innexin OS=Taenia asiatica OX=60517 GN=inx PE=3 SV=1)

HSP 1 Score: 117.087 bits (292), Expect = 1.824e-26
Identity = 61/150 (40.67%), Postives = 95/150 (63.33%), Query Frame = 2 
Query:    2 FLWFWFVFIAIITVHSFIHWTSKSVCRIQRYNFIRKFLVLKDALQEAGDRKKLYAFIDLFLQQDGVFILRLLALNCGDLIAAEITEYLWCRFKECARMDSGERSKLFYLD-----SGDVYSKARPYRSRKSKDLIEPVQSRRRIPSAPAL 436
            FLWFWF+ ++ +++ SF++W+ KS  R  R +FIRKFL L+D+L E G++K+ YAF+D +L+QDGVFILRL+A N G+L+A+EI E LW  FK    + + E+ +   +      SG  +  + P R+ +S +L  P        +AP +
Sbjct:  295 FLWFWFILVSAVSISSFLYWSYKSSFRSNRIHFIRKFLKLRDSL-EPGEKKRSYAFVDSYLRQDGVFILRLVAQNAGELVASEIVEKLWQMFKRRQSLPTTEKVRANEVGWKSGVSGHSHIHSPPMRAPRSSEL-SPTSVTSPTINAPTV 442
[Close]
BLAST of Innexin vs. TrEMBL
Match: A0A504YG67 (Innexin OS=Fasciola gigantica OX=46835 GN=inx PE=3 SV=1)

HSP 1 Score: 115.161 bits (287), Expect = 6.059e-26
Identity = 52/93 (55.91%), Postives = 71/93 (76.34%), Query Frame = 2 
Query:    2 FLWFWFVFIAIITVHSFIHWTSKSVCRIQRYNFIRKFLVLKDALQEAGDRKKLYAFIDLFLQQDGVFILRLLALNCGDLIAAEITEYLWCRFK 280
            FLWFWF+ +A + + SF++W+ KS     R +FIRKFL L+DAL E GD+K+ +AF+D +L+QDGVFILRL+A N G+L+A+EI E LW  FK
Sbjct:  155 FLWFWFILVAAVNISSFLYWSYKSSFHGNRVHFIRKFLKLRDAL-EPGDKKRTFAFVDSYLRQDGVFILRLVAQNAGELVASEIVERLWNMFK 246
[Close]
BLAST of Innexin vs. TrEMBL
Match: A0A0R3TP98 (Innexin OS=Rodentolepis nana OX=102285 GN=inx PE=3 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 2.027e-25
Identity = 51/103 (49.51%), Postives = 77/103 (74.76%), Query Frame = 2 
Query:    2 FLWFWFVFIAIITVHSFIHWTSKSVCRIQRYNFIRKFLVLKDALQEAGDRKKLYAFIDLFLQQDGVFILRLLALNCGDLIAAEITEYLWCRFKECARMDSGER 310
            FLWFWF+ ++ +++ SF++W+ KS  R  R +FIRKFL L+D+L E G++K+ YAF+D +L+QDGVFILRL+A N G+L+A+EI E LW  +K    + + E+
Sbjct:  155 FLWFWFILVSAVSISSFLYWSYKSSFRSNRIHFIRKFLKLRDSL-EPGEKKRTYAFVDSYLRQDGVFILRLVAQNAGELVASEIVEKLWIIYKRRQLLPTTEK 256
[Close]
BLAST of Innexin vs. Planmine SMEST
Match: SMESG000035987.1 (SMESG000035987.1)

HSP 1 Score: 343.584 bits (880), Expect = 4.038e-117
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 2 
Query:    2 FLWFWFVFIAIITVHSFIHWTSKSVCRIQRYNFIRKFLVLKDALQEAGDRKKLYAFIDLFLQQDGVFILRLLALNCGDLIAAEITEYLWCRFKECARMDSGERSKLFYLDSGDVYSKARPYRSRKSKDLIEPVQSRRRIPSAPALKEXXXXXXXXXXXXXXXXXXXXXXRPPKKMSCANLHHVSEEDPGEFV 577
            FLWFWFVFIAIITVHSFIHWTSKSVCRIQRYNFIRKFLVLKDALQEAGDRKKLYAFIDLFLQQDGVFILRLLALNCGDLIAAEITEYLWCRFKECARMDSGERSKLFYLDSGDVYSKARPYRSRKSKDLIEPVQSRRRIPSAPALKENSHSQSNSNTSSSNRSNISAPNRPPKKMSCANLHHVSEEDPGEFV
Sbjct:  295 FLWFWFVFIAIITVHSFIHWTSKSVCRIQRYNFIRKFLVLKDALQEAGDRKKLYAFIDLFLQQDGVFILRLLALNCGDLIAAEITEYLWCRFKECARMDSGERSKLFYLDSGDVYSKARPYRSRKSKDLIEPVQSRRRIPSAPALKENSHSQSNSNTSSSNRSNISAPNRPPKKMSCANLHHVSEEDPGEFV 486
[Close]
BLAST of Innexin vs. Planmine SMEST
Match: SMESG000046667.1 (SMESG000046667.1)

HSP 1 Score: 87.4261 bits (215), Expect = 1.133e-19
Identity = 66/192 (34.38%), Postives = 92/192 (47.92%), Query Frame = 2 
Query:    2 FLWFWFVFIAIITVHSFIHWTSKSVCRIQRYNFIRKFLVLKDALQEAGDRKKLYAFIDLFLQQDGVFILRLLALNCGDLIAAEITEYLWCRFKECARMDSGERSKLFYLDSGDVYSKARPYRSRKSKDLIEPVQSRRRIPSAPALKEXXXXXXXXXXXXXXXXXXXXXXRPPKKMSCANLHHVSEEDPGEFV 577
            FLWFWFV ++ +TV SFI  T K      R NF+ KFL L+    E   + K   F++  L+ DGVF+LRLLA N GDLIA+EIT+ LW       R+ S  +S+       D++     +R+  S   I        +   P L E+  S    ++S +                 A  +  +EEDPGEFV
Sbjct:  309 FLWFWFVLVSAVTVSSFISLTYKIGLVYNRINFVCKFLKLRQK-PETITKNKCRNFVEKHLRHDGVFLLRLLAQNAGDLIASEITDSLW-------RLYSDHKSE----TKTDLFRSKENHRNLASGQQI--------LTHKPQLNESLLSLRKDSSSHTKIRGPDKSRHKLPLNEFATANPTAEEDPGEFV 480
[Close]
BLAST of Innexin vs. Planmine SMEST
Match: SMESG000046667.1 (SMESG000046667.1)

HSP 1 Score: 87.4261 bits (215), Expect = 1.269e-19
Identity = 66/192 (34.38%), Postives = 92/192 (47.92%), Query Frame = 2 
Query:    2 FLWFWFVFIAIITVHSFIHWTSKSVCRIQRYNFIRKFLVLKDALQEAGDRKKLYAFIDLFLQQDGVFILRLLALNCGDLIAAEITEYLWCRFKECARMDSGERSKLFYLDSGDVYSKARPYRSRKSKDLIEPVQSRRRIPSAPALKEXXXXXXXXXXXXXXXXXXXXXXRPPKKMSCANLHHVSEEDPGEFV 577
            FLWFWFV ++ +TV SFI  T K      R NF+ KFL L+    E   + K   F++  L+ DGVF+LRLLA N GDLIA+EIT+ LW       R+ S  +S+       D++     +R+  S   I        +   P L E+  S    ++S +                 A  +  +EEDPGEFV
Sbjct:  295 FLWFWFVLVSAVTVSSFISLTYKIGLVYNRINFVCKFLKLRQK-PETITKNKCRNFVEKHLRHDGVFLLRLLAQNAGDLIASEITDSLW-------RLYSDHKSE----TKTDLFRSKENHRNLASGQQI--------LTHKPQLNESLLSLRKDSSSHTKIRGPDKSRHKLPLNEFATANPTAEEDPGEFV 466
[Close]
BLAST of Innexin vs. Planmine SMEST
Match: SMESG000030970.1 (SMESG000030970.1)

HSP 1 Score: 73.559 bits (179), Expect = 4.058e-15
Identity = 37/108 (34.26%), Postives = 59/108 (54.63%), Query Frame = 2 
Query:    2 FLWFWFVFIAIITVHSFIHWTSKSVCRIQRYNFIRKFLVLKD--ALQEAGDRKKLYAFIDLFLQQDGVFILRLLALNCGDLIAAEITEYLWCRFKECARMDSGERSKL 319
            FLWFW + +++I++ S   W  + +    + +F+  +L L      +E  D+KK+  F  +FL+ DG+F LR+LA N GDLI AE+   LW  +  C      +  KL
Sbjct:  180 FLWFWMLILSVISITSLFMWLIRMLISRNKTDFLNMYLKLSGDYTHRELKDKKKIVKFEKIFLRNDGMFFLRILATNVGDLITAEVVSQLWKIYAPCYENTELDMEKL 287
[Close]
BLAST of Innexin vs. Planmine SMEST
Match: SMESG000030970.1 (SMESG000030970.1)

HSP 1 Score: 73.559 bits (179), Expect = 6.981e-15
Identity = 37/108 (34.26%), Postives = 59/108 (54.63%), Query Frame = 2 
Query:    2 FLWFWFVFIAIITVHSFIHWTSKSVCRIQRYNFIRKFLVLKD--ALQEAGDRKKLYAFIDLFLQQDGVFILRLLALNCGDLIAAEITEYLWCRFKECARMDSGERSKL 319
            FLWFW + +++I++ S   W  + +    + +F+  +L L      +E  D+KK+  F  +FL+ DG+F LR+LA N GDLI AE+   LW  +  C      +  KL
Sbjct:  298 FLWFWMLILSVISITSLFMWLIRMLISRNKTDFLNMYLKLSGDYTHRELKDKKKIVKFEKIFLRNDGMFFLRILATNVGDLITAEVVSQLWKIYAPCYENTELDMEKL 405
The following BLAST results are available for this feature:
BLAST of Innexin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
inx-14.842e-1437.11Innexin [Source:UniProtKB/TrEMBL;Acc:Q17394][more]
inx-11.013e-1337.11Innexin [Source:UniProtKB/TrEMBL;Acc:Q17394][more]
inx-11.013e-1337.11Innexin [Source:UniProtKB/TrEMBL;Acc:Q17394][more]
unc-71.887e-1236.36Innexin unc-7 [Source:UniProtKB/Swiss-Prot;Acc:Q0... [more]
unc-72.012e-1236.36Innexin unc-7 [Source:UniProtKB/Swiss-Prot;Acc:Q0... [more]
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BLAST of Innexin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q03412|UNC7_CAEEL3.092e-1136.36Innexin unc-7 OS=Caenorhabditis elegans OX=6239 GN... [more]
sp|O61715|INX19_CAEEL1.604e-1028.32Innexin-19 OS=Caenorhabditis elegans OX=6239 GN=in... [more]
sp|O01393|UNC9_CAEEL3.958e-1036.27Innexin unc-9 OS=Caenorhabditis elegans OX=6239 GN... [more]
sp|A8WVX4|INX19_CAEBR5.310e-1026.55Innexin-19 OS=Caenorhabditis briggsae OX=6238 GN=i... [more]
sp|Q22549|INX10_CAEEL2.536e-931.58Innexin-10 OS=Caenorhabditis elegans OX=6239 GN=in... [more]
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BLAST of Innexin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
W6V7B33.678e-2743.28Innexin OS=Echinococcus granulosus OX=6210 GN=inx ... [more]
A0A0R3X3I04.613e-2741.33Innexin OS=Hydatigena taeniaeformis OX=6205 GN=inx... [more]
A0A158R8191.824e-2640.67Innexin OS=Taenia asiatica OX=60517 GN=inx PE=3 SV... [more]
A0A504YG676.059e-2655.91Innexin OS=Fasciola gigantica OX=46835 GN=inx PE=3... [more]
A0A0R3TP982.027e-2549.51Innexin OS=Rodentolepis nana OX=102285 GN=inx PE=3... [more]
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BLAST of Innexin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Innexin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000035987.14.038e-117100.00SMESG000035987.1[more]
SMESG000046667.11.133e-1934.38SMESG000046667.1[more]
SMESG000046667.11.269e-1934.38SMESG000046667.1[more]
SMESG000030970.14.058e-1534.26SMESG000030970.1[more]
SMESG000030970.16.981e-1534.26SMESG000030970.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024751 ID=SMED30024751|Name=Innexin|organism=Schmidtea mediterranea sexual|type=transcript|length=721bp
TTTTCTTTGGTTTTGGTTTGTTTTTATCGCGATCATAACTGTCCATAGTT
TTATTCACTGGACATCGAAATCGGTTTGTCGAATACAACGATATAATTTC
ATTAGAAAATTTTTAGTGCTTAAAGACGCCTTACAAGAAGCTGGAGATCG
GAAAAAACTTTATGCCTTTATAGATTTATTTCTTCAACAGGATGGTGTTT
TTATTTTGCGATTATTGGCATTGAATTGCGGTGATTTGATTGCTGCTGAA
ATCACCGAATATCTCTGGTGTCGGTTCAAAGAGTGTGCACGAATGGATTC
CGGAGAAAGATCAAAATTATTTTATTTAGACAGTGGAGATGTGTACTCAA
AAGCAAGACCATACAGATCTAGAAAATCCAAAGACTTAATTGAACCTGTT
CAATCTCGTCGGAGAATTCCGAGTGCTCCCGCTTTAAAAGAAAATTCGCA
TTCACAAAGCAATAGCAATACGTCGAGTTCAAACCGGTCAAATATTTCCG
CCCCAAATCGTCCACCAAAGAAAATGTCTTGTGCCAATTTGCATCATGTA
AGTGAGGAAGATCCAGGAGAGTTTGTTTAATGGATTTTGTTGCATTAAAT
TTACTTTGCATGAGTTTATTTTTAATTTGATTTATTGGAAACATTTATTT
AAGAAAACCGATTTATTTTTCTGTAAATTATGTGACTTTGTTCATTGAAA
CATGGTTCCATTATTTTTTCG
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protein sequence of SMED30024751-orf-1

>SMED30024751-orf-1 ID=SMED30024751-orf-1|Name=SMED30024751-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=193bp
FLWFWFVFIAIITVHSFIHWTSKSVCRIQRYNFIRKFLVLKDALQEAGDR
KKLYAFIDLFLQQDGVFILRLLALNCGDLIAAEITEYLWCRFKECARMDS
GERSKLFYLDSGDVYSKARPYRSRKSKDLIEPVQSRRRIPSAPALKENSH
SQSNSNTSSSNRSNISAPNRPPKKMSCANLHHVSEEDPGEFV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR000990Innexin
Vocabulary: cellular component
TermDefinition
GO:0005921gap junction
GO:0005886plasma membrane
GO:0016020membrane
GO:0016021integral component of membrane
GO:0030054cell junction
Vocabulary: biological process
TermDefinition
GO:0006810transport
GO:0006811ion transport
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000990InnexinPFAMPF00876Innexincoord: 1..91
e-value: 6.1E-19
score: 68.5
IPR000990InnexinPANTHERPTHR11893INNEXINcoord: 1..97
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 145..170
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 140..192
NoneNo IPR availablePANTHERPTHR11893:SF36INNEXIN-5coord: 1..97
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..16
score: 0.856
NoneNo IPR availableTMHMMTMhelixcoord: 4..26