SMED30024671

Overview
NameSMED30024671
Smed IDSMED30024671
Length (bp)385
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30024671 (SMED30024671) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30024671 (SMED30024671) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30024671 (SMED30024671) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30024671 (SMED30024671) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30024671

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30024671 SmedASXL_018917SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30024671 SmedASXL_018917SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
nervous systemSMED30024671 dd_Smed_v4_1516_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30024671 dd_Smed_v4_1516_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30024671 dd_Smed_v4_1516_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30024671 dd_Smed_v4_1516_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30024671 dd_Smed_v4_1516_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30024671 SMED30024671smed_20140614PMID:26457503
Tu et al., 2015		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30024671 SMED30024671smed_20140614PMID:26457503
Tu et al., 2015		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Alignments
SMED30024671 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000560:35715..36185 -1861
Homology
[Close]
BLAST of SMED30024671 vs. Ensembl Human
Match: TIMM13 (translocase of inner mitochondrial membrane 13 [Source:HGNC Symbol;Acc:HGNC:11816])

HSP 1 Score: 84.7297 bits (208), Expect = 5.415e-22
Identity = 36/72 (50.00%), Postives = 53/72 (73.61%), Query Frame = -2 
Query:   91 LMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESS 306
            +MEQVK Q A+   ++L+Q +TDKCF KC+ K G SLD+S+ KC   CM+RYM+A N V++ Y++RL +E +
Sbjct:   22 IMEQVKVQIAVANAQELLQRMTDKCFRKCIGKPGGSLDNSEQKCIAMCMDRYMDAWNTVSRAYNSRLQRERA 93
[Close]
BLAST of SMED30024671 vs. Ensembl Human
Match: TIMM13 (translocase of inner mitochondrial membrane 13 [Source:HGNC Symbol;Acc:HGNC:11816])

HSP 1 Score: 68.5514 bits (166), Expect = 8.529e-16
Identity = 28/55 (50.91%), Postives = 40/55 (72.73%), Query Frame = -2 
Query:   88 QTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESSQ 252
            Q +TDKCF KC+ K G SLD+S+ KC   CM+RYM+A N V++ Y++RL +E + 
Sbjct:   25 QRMTDKCFRKCIGKPGGSLDNSEQKCIAMCMDRYMDAWNTVSRAYNSRLQRERAN 79
[Close]
BLAST of SMED30024671 vs. Ensembl Celegans
Match: tin-13 (Mitochondrial import inner membrane translocase subunit tim-13 [Source:UniProtKB/Swiss-Prot;Acc:O45319])

HSP 1 Score: 71.633 bits (174), Expect = 3.580e-17
Identity = 32/78 (41.03%), Postives = 55/78 (70.51%), Query Frame = -2 
Query:   97 LTDNQKEKLMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKE 330
            L+  Q+E+++  VK QAA+   ++L+  I++KC +KC+T  G+SL S + +C  +CM+R+ME+ NLV++T   RL +E
Sbjct:   13 LSPEQQEQVISGVKQQAALANAQNLVTDISEKCTNKCITAPGSSLASGEKQCLQRCMDRFMESWNLVSQTLQKRLQEE 90
[Close]
BLAST of SMED30024671 vs. Ensembl Fly
Match: CG34132 (gene:FBgn0083968 transcript:FBtr0110879)

HSP 1 Score: 85.8853 bits (211), Expect = 6.130e-23
Identity = 37/83 (44.58%), Postives = 59/83 (71.08%), Query Frame = -2 
Query:   85 ALTDNQKEKLMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESSQY 333
            A+ +  K +LM+QVK Q A+   ++L+  +T+KCF KCV K GTSLDSS+ KC   CM+R+M++ NL+++ Y  R+ +E S++
Sbjct:    2 AMANVDKGELMDQVKQQIAVANAQELLTQMTEKCFKKCVNKPGTSLDSSEQKCISMCMDRFMDSWNLISRVYGQRIQREQSKF 84
[Close]
BLAST of SMED30024671 vs. Ensembl Fly
Match: Tim13 (gene:FBgn0036204 transcript:FBtr0076089)

HSP 1 Score: 82.8037 bits (203), Expect = 1.364e-21
Identity = 36/79 (45.57%), Postives = 56/79 (70.89%), Query Frame = -2 
Query:   97 ALTDNQKEKLMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKE 333
            A  + +K +LM QVK Q A+   ++++  +T+KCF KC+ K G SLDS++ +C  QCM+R+M+A NLV++TY  RL +E
Sbjct:    2 AAANMEKGELMNQVKQQIALANAQEMLSKMTEKCFKKCIQKPGKSLDSTEQRCISQCMDRFMDAWNLVSRTYGNRLQRE 80
[Close]
BLAST of SMED30024671 vs. Ensembl Fly
Match: CG42302 (gene:FBgn0259198 transcript:FBtr0299682)

HSP 1 Score: 47.3654 bits (111), Expect = 1.433e-7
Identity = 18/62 (29.03%), Postives = 38/62 (61.29%), Query Frame = -2 
Query:  118 MEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTY 303
            +E+++ Q  +  I++L++ +T +CFD C+      L S++  C   CM+R+M++  +V+  Y
Sbjct:    8 LERIRQQIVLANIQELIKKMTRRCFDVCIAMPEMELRSTERDCLANCMDRFMDSVQVVSSQY 69
[Close]
BLAST of SMED30024671 vs. Ensembl Fly
Match: Tim8 (gene:FBgn0027359 transcript:FBtr0343640)

HSP 1 Score: 42.3578 bits (98), Expect = 6.515e-6
Identity = 22/84 (26.19%), Postives = 41/84 (48.81%), Query Frame = -2 
Query:   91 DMSALTDNQKEKLMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESS 342
            D   L+ N KE L E +  +    Q+   +    + C++KC+ K  T LD +   C   C++R+++   L+T+ ++  L K   
Sbjct:    3 DFENLSGNDKE-LQEFLLIEKQKAQVNAQIHEFNEICWEKCIGKPSTKLDHATETCLSNCVDRFIDTSLLITQRFAQMLQKRGG 85
[Close]
BLAST of SMED30024671 vs. Ensembl Fly
Match: Tim8 (gene:FBgn0027359 transcript:FBtr0073482)

HSP 1 Score: 42.3578 bits (98), Expect = 6.515e-6
Identity = 22/84 (26.19%), Postives = 41/84 (48.81%), Query Frame = -2 
Query:   91 DMSALTDNQKEKLMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESS 342
            D   L+ N KE L E +  +    Q+   +    + C++KC+ K  T LD +   C   C++R+++   L+T+ ++  L K   
Sbjct:    3 DFENLSGNDKE-LQEFLLIEKQKAQVNAQIHEFNEICWEKCIGKPSTKLDHATETCLSNCVDRFIDTSLLITQRFAQMLQKRGG 85
[Close]
BLAST of SMED30024671 vs. Ensembl Zebrafish
Match: timm13 (translocase of inner mitochondrial membrane 13 homolog (yeast) [Source:NCBI gene;Acc:436738])

HSP 1 Score: 83.9593 bits (206), Expect = 5.936e-22
Identity = 35/73 (47.95%), Postives = 54/73 (73.97%), Query Frame = -2 
Query:   88 LMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESSQ 306
            +MEQVK Q A+   ++L+Q +TDKCF KC+ K G++LD+S+ KC   CM+RYM+A N V++ Y++RL +E + 
Sbjct:   22 IMEQVKVQIAVANAQELLQRMTDKCFKKCIGKPGSTLDNSEQKCIAMCMDRYMDAWNTVSRAYNSRLQRERAH 94
[Close]
BLAST of SMED30024671 vs. Ensembl Xenopus
Match: timm13 (translocase of inner mitochondrial membrane 13 homolog [Source:NCBI gene;Acc:734099])

HSP 1 Score: 85.1149 bits (209), Expect = 3.194e-22
Identity = 36/73 (49.32%), Postives = 54/73 (73.97%), Query Frame = -2 
Query:   88 LMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESSQ 306
            +MEQVK Q A+   ++L+Q +TDKCF KC+ K G SLD+S+ KC   CM+RYM+A N V++ Y++RL +E ++
Sbjct:   23 IMEQVKVQIAVANAQELLQRMTDKCFRKCIGKPGGSLDNSEQKCIAMCMDRYMDAWNTVSRAYNSRLQRERAK 95
[Close]
BLAST of SMED30024671 vs. Ensembl Xenopus
Match: timm13 (translocase of inner mitochondrial membrane 13 homolog [Source:NCBI gene;Acc:734099])

HSP 1 Score: 68.9366 bits (167), Expect = 3.496e-14
Identity = 28/55 (50.91%), Postives = 41/55 (74.55%), Query Frame = -2 
Query:   88 QTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESSQ 252
            Q +TDKCF KC+ K G SLD+S+ KC   CM+RYM+A N V++ Y++RL +E ++
Sbjct:  610 QRMTDKCFRKCIGKPGGSLDNSEQKCIAMCMDRYMDAWNTVSRAYNSRLQRERAK 664
[Close]
BLAST of SMED30024671 vs. Ensembl Mouse
Match: Timm13 (translocase of inner mitochondrial membrane 13 [Source:MGI Symbol;Acc:MGI:1353432])

HSP 1 Score: 84.7297 bits (208), Expect = 3.334e-22
Identity = 36/73 (49.32%), Postives = 53/73 (72.60%), Query Frame = -2 
Query:   88 LMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESSQ 306
            +MEQVK Q A+   ++L+Q +TDKCF KC+ K G SLD+S+ KC   CM+RYM+A N V++ Y++RL +E + 
Sbjct:   22 IMEQVKVQIAVANAQELLQRMTDKCFRKCIGKPGGSLDNSEQKCIAMCMDRYMDAWNTVSRAYNSRLQRERAN 94
[Close]
BLAST of SMED30024671 vs. Ensembl Mouse
Match: Timm13 (translocase of inner mitochondrial membrane 13 [Source:MGI Symbol;Acc:MGI:1353432])

HSP 1 Score: 68.5514 bits (166), Expect = 4.465e-16
Identity = 31/65 (47.69%), Postives = 44/65 (67.69%), Query Frame = -2 
Query:   88 AAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESSQ 282
             AIM+     Q +TDKCF KC+ K G SLD+S+ KC   CM+RYM+A N V++ Y++RL +E + 
Sbjct:   20 GAIME-----QRMTDKCFRKCIGKPGGSLDNSEQKCIAMCMDRYMDAWNTVSRAYNSRLQRERAN 79
[Close]
BLAST of SMED30024671 vs. Ensembl Mouse
Match: Timm13 (translocase of inner mitochondrial membrane 13 [Source:MGI Symbol;Acc:MGI:1353432])

HSP 1 Score: 50.8322 bits (120), Expect = 3.816e-9
Identity = 22/41 (53.66%), Postives = 31/41 (75.61%), Query Frame = -2 
Query:  184 LMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQ 306
            +MEQVK Q A+   ++L+Q +TDKCF KC+ K G SLD+S+
Sbjct:   22 IMEQVKVQIAVANAQELLQRMTDKCFRKCIGKPGGSLDNSE 62
[Close]
BLAST of SMED30024671 vs. UniProt/SwissProt
Match: sp|Q8AVK1|TI13B_XENLA (Mitochondrial import inner membrane translocase subunit Tim13-B OS=Xenopus laevis OX=8355 GN=timm13-b PE=3 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 2.518e-22
Identity = 36/73 (49.32%), Postives = 55/73 (75.34%), Query Frame = -2 
Query:   88 LMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESSQ 306
            +MEQVK Q A+   ++L+Q +TDKCF KC+ K G SLD+S+ KC   CM+RYM+A N+V++ Y++RL +E ++
Sbjct:   23 IMEQVKVQIAVANAQELLQRMTDKCFRKCIGKPGGSLDNSEQKCVAMCMDRYMDAWNIVSRAYNSRLQRERAK 95
[Close]
BLAST of SMED30024671 vs. UniProt/SwissProt
Match: sp|Q6GPY0|TI13A_XENLA (Mitochondrial import inner membrane translocase subunit Tim13-A OS=Xenopus laevis OX=8355 GN=timm13-a PE=3 SV=2)

HSP 1 Score: 85.1149 bits (209), Expect = 1.620e-21
Identity = 36/73 (49.32%), Postives = 54/73 (73.97%), Query Frame = -2 
Query:   88 LMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESSQ 306
            +MEQVK Q A+   ++L+Q +TDKCF KC+ K G SLD+S+ KC   CM+RYM+A N V++ Y++RL +E ++
Sbjct:   23 IMEQVKVQIAVANAQELLQRMTDKCFRKCIGKPGGSLDNSEQKCIAMCMDRYMDAWNTVSRAYNSRLQRERAK 95
[Close]
BLAST of SMED30024671 vs. UniProt/SwissProt
Match: sp|P62076|TIM13_RAT (Mitochondrial import inner membrane translocase subunit Tim13 OS=Rattus norvegicus OX=10116 GN=Timm13 PE=3 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 2.332e-21
Identity = 36/73 (49.32%), Postives = 53/73 (72.60%), Query Frame = -2 
Query:   88 LMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESSQ 306
            +MEQVK Q A+   ++L+Q +TDKCF KC+ K G SLD+S+ KC   CM+RYM+A N V++ Y++RL +E + 
Sbjct:   22 IMEQVKVQIAVANAQELLQRMTDKCFRKCIGKPGGSLDNSEQKCIAMCMDRYMDAWNTVSRAYNSRLQRERAN 94
[Close]
BLAST of SMED30024671 vs. UniProt/SwissProt
Match: sp|P62075|TIM13_MOUSE (Mitochondrial import inner membrane translocase subunit Tim13 OS=Mus musculus OX=10090 GN=Timm13 PE=1 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 2.332e-21
Identity = 36/73 (49.32%), Postives = 53/73 (72.60%), Query Frame = -2 
Query:   88 LMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESSQ 306
            +MEQVK Q A+   ++L+Q +TDKCF KC+ K G SLD+S+ KC   CM+RYM+A N V++ Y++RL +E + 
Sbjct:   22 IMEQVKVQIAVANAQELLQRMTDKCFRKCIGKPGGSLDNSEQKCIAMCMDRYMDAWNTVSRAYNSRLQRERAN 94
[Close]
BLAST of SMED30024671 vs. UniProt/SwissProt
Match: sp|Q9Y5L4|TIM13_HUMAN (Mitochondrial import inner membrane translocase subunit Tim13 OS=Homo sapiens OX=9606 GN=TIMM13 PE=1 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 2.600e-21
Identity = 36/72 (50.00%), Postives = 53/72 (73.61%), Query Frame = -2 
Query:   91 LMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESS 306
            +MEQVK Q A+   ++L+Q +TDKCF KC+ K G SLD+S+ KC   CM+RYM+A N V++ Y++RL +E +
Sbjct:   22 IMEQVKVQIAVANAQELLQRMTDKCFRKCIGKPGGSLDNSEQKCIAMCMDRYMDAWNTVSRAYNSRLQRERA 93
[Close]
BLAST of SMED30024671 vs. TrEMBL
Match: A0A5J4NSX8 (Mitochondrial import inner membrane translocase subunit TIM13 OS=Paragonimus westermani OX=34504 GN=DEA37_0003718 PE=3 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 3.578e-26
Identity = 43/84 (51.19%), Postives = 71/84 (84.52%), Query Frame = -2 
Query:   94 MDMSALTDNQKEKLMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKES 345
            +++S+LT  QKE+LMEQ+KA+ A++  ++L+Q ++DKCF+KCV K GT+LDSS+ KC G CM+RY++A NLV++T+++R+ +E+
Sbjct:    8 LNLSSLTTAQKEQLMEQMKAEVALVSAQELLQKMSDKCFEKCVAKPGTTLDSSEQKCVGLCMDRYVDAWNLVSRTFASRIRRET 91
[Close]
BLAST of SMED30024671 vs. TrEMBL
Match: G7YIW8 (Mitochondrial import inner membrane translocase subunit Tim13-B OS=Clonorchis sinensis OX=79923 GN=timm13-b PE=3 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 4.458e-25
Identity = 42/83 (50.60%), Postives = 67/83 (80.72%), Query Frame = -2 
Query:   97 MDMSALTDNQKEKLMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKE 345
            +++S+LT  QKE+LMEQ+KA+ A+   ++L+Q ++DKCF+KCV K G SLD+S+ KC G CM+RY++A NLV++T++ R+ +E
Sbjct:    8 LNLSSLTSAQKEQLMEQMKAEVALASAQELLQRMSDKCFEKCVPKPGLSLDNSEQKCVGMCMDRYVDAWNLVSRTFAARVRRE 90
[Close]
BLAST of SMED30024671 vs. TrEMBL
Match: A0A1S8WUV2 (Tim10/DDP family zinc finger (Fragment) OS=Opisthorchis viverrini OX=6198 GN=X801_05916 PE=3 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 5.545e-25
Identity = 41/84 (48.81%), Postives = 68/84 (80.95%), Query Frame = -2 
Query:   94 MDMSALTDNQKEKLMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKES 345
            +++++LT  QKE+LMEQ+KA+ A+   ++L+Q ++DKCF+KCV K G SLD+S+ KC G CM+RY++A NLV++T++ R+ +E+
Sbjct:    8 LNLNSLTSAQKEQLMEQMKAEVALASAQELLQRMSDKCFEKCVPKPGVSLDNSEQKCVGMCMDRYVDAWNLVSRTFAARVRREA 91
[Close]
BLAST of SMED30024671 vs. TrEMBL
Match: A0A4Z2CNF7 (Mitochondrial import inner membrane translocase subunit Tim13-B OS=Schistosoma japonicum OX=6182 GN=EWB00_009142 PE=3 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 8.698e-25
Identity = 42/86 (48.84%), Postives = 68/86 (79.07%), Query Frame = -2 
Query:   88 MDMSALTDNQKEKLMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESSQ 345
            +++S+LT  QK +LMEQ+K + A+   R+L++ ++DKCF+KCVTK GTSLD+S+ KC G CM+RY++A NLV+K ++ R+ +E+ +
Sbjct:    5 VNLSSLTPAQKGQLMEQMKTEVALASARELLEKMSDKCFEKCVTKPGTSLDNSEQKCVGLCMDRYIDAWNLVSKVFAARIKREAEK 90
[Close]
BLAST of SMED30024671 vs. TrEMBL
Match: A0A183VJZ4 (zf-Tim10_DDP domain-containing protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS329 PE=3 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 9.359e-25
Identity = 41/86 (47.67%), Postives = 69/86 (80.23%), Query Frame = -2 
Query:   88 MDMSALTDNQKEKLMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESSQ 345
            +++++LT  QK +LMEQ++A+ A+   R+L++ ++DKCF+KCV+K GTSLD+S+ KC G CM+RY+EA NLV+K ++ R+ +E+ +
Sbjct:    5 INLNSLTPAQKGQLMEQMRAEVALASARELLEKMSDKCFEKCVSKPGTSLDNSEQKCIGLCMDRYVEAWNLVSKVFAARIRREAEK 90
[Close]
BLAST of SMED30024671 vs. Ensembl Cavefish
Match: timm13 (translocase of inner mitochondrial membrane 13 [Source:NCBI gene;Acc:103034199])

HSP 1 Score: 84.7297 bits (208), Expect = 3.049e-22
Identity = 35/73 (47.95%), Postives = 55/73 (75.34%), Query Frame = -2 
Query:   88 LMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESSQ 306
            +MEQVK Q A+   ++L+Q +TDKCF KC+ K G++LD+S+ KC   CM+RYM+A N V++ Y++RL +E ++
Sbjct:   22 IMEQVKVQIAVANAQELLQRMTDKCFKKCIGKPGSTLDNSEQKCIAMCMDRYMDAWNTVSRAYNSRLQRERAR 94
[Close]
BLAST of SMED30024671 vs. Ensembl Cavefish
Match: timm13 (translocase of inner mitochondrial membrane 13 [Source:NCBI gene;Acc:103034199])

HSP 1 Score: 49.6766 bits (117), Expect = 1.666e-8
Identity = 21/41 (51.22%), Postives = 32/41 (78.05%), Query Frame = -2 
Query:  184 LMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQ 306
            +MEQVK Q A+   ++L+Q +TDKCF KC+ K G++LD+S+
Sbjct:   22 IMEQVKVQIAVANAQELLQRMTDKCFKKCIGKPGSTLDNSE 62
[Close]
BLAST of SMED30024671 vs. Ensembl Yeast
Match: TIM13 (Mitochondrial intermembrane space protein; forms a complex with Tim8p that delivers a subset of hydrophobic proteins to the TIM22 complex for insertion into the inner membrane [Source:SGD;Acc:S000003413])

HSP 1 Score: 47.7506 bits (112), Expect = 1.065e-8
Identity = 20/74 (27.03%), Postives = 42/74 (56.76%), Query Frame = -2 
Query:   91 EKLMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESS 312
            ++L  Q+  + A+    +L+  I++ CF+KC+T   +   +    C  QC+ +YM + N+++K Y +R+   S+
Sbjct:   31 KELKNQIAQELAVANATELVNKISENCFEKCLT---SPYATRNDACIDQCLAKYMRSWNVISKAYISRIQNASA 101
[Close]
BLAST of SMED30024671 vs. Ensembl Nematostella
Match: EDO33527 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SRZ6])

HSP 1 Score: 82.0333 bits (201), Expect = 6.313e-22
Identity = 36/71 (50.70%), Postives = 51/71 (71.83%), Query Frame = -2 
Query:   91 MEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESS 303
            M+QVK Q A+   ++L+Q I+DKCF KC+ K G SLD+S+ KC   CM+RYM++ N V++ Y+ RL KE S
Sbjct:    1 MDQVKTQIALANAQELLQKISDKCFKKCIFKPGASLDNSEQKCIALCMDRYMDSWNTVSRVYTARLQKEHS 71
[Close]
BLAST of SMED30024671 vs. Ensembl Nematostella
Match: EDO44492 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVQ3])

HSP 1 Score: 43.8986 bits (102), Expect = 5.001e-7
Identity = 23/78 (29.49%), Postives = 41/78 (52.56%), Query Frame = -2 
Query:   85 QKEKLMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESSQY 318
            ++E ++E+ KA+   MQ+       TD C+DKC+ K G  LD     C   C+ER+++   L+TK +   + +    +
Sbjct:   12 RQELMIEEQKAKFQ-MQV----HKFTDTCWDKCMDKPGNKLDGKTETCLVNCVERFIDTTLLITKRFGELVQRSGGGF 84
[Close]
BLAST of SMED30024671 vs. Ensembl Nematostella
Match: EDO26210 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVQ3])

HSP 1 Score: 43.8986 bits (102), Expect = 5.001e-7
Identity = 23/78 (29.49%), Postives = 41/78 (52.56%), Query Frame = -2 
Query:   85 QKEKLMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESSQY 318
            ++E ++E+ KA+   MQ+       TD C+DKC+ K G  LD     C   C+ER+++   L+TK +   + +    +
Sbjct:   12 RQELMIEEQKAKFQ-MQV----HKFTDTCWDKCMDKPGNKLDGKTETCLVNCVERFIDTTLLITKRFGELVQRSGGGF 84
[Close]
BLAST of SMED30024671 vs. Ensembl Medaka
Match: timm13 (translocase of inner mitochondrial membrane 13 [Source:NCBI gene;Acc:101175143])

HSP 1 Score: 88.1965 bits (217), Expect = 1.182e-23
Identity = 38/73 (52.05%), Postives = 56/73 (76.71%), Query Frame = -2 
Query:   88 LMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESSQ 306
            +MEQVK Q A+   ++L+Q +TDKCF KC+ K G+SLD+S+ KC   CM+RYM+A N V++TY++RL KE ++
Sbjct:   22 IMEQVKVQIAVANAQELLQRMTDKCFKKCIGKPGSSLDNSEQKCIAMCMDRYMDAWNTVSRTYNSRLQKERAR 94
[Close]
BLAST of SMED30024671 vs. Planmine SMEST
Match: SMESG000017034.1 (SMESG000017034.1)

HSP 1 Score: 182.956 bits (463), Expect = 2.847e-61
Identity = 89/89 (100.00%), Postives = 89/89 (100.00%), Query Frame = -2 
Query:   79 MDMSALTDNQKEKLMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESSQYGA 345
            MDMSALTDNQKEKLMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESSQYGA
Sbjct:    1 MDMSALTDNQKEKLMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESSQYGA 89
[Close]
BLAST of SMED30024671 vs. Planmine SMEST
Match: SMESG000017034.1 (SMESG000017034.1)

HSP 1 Score: 179.104 bits (453), Expect = 8.677e-60
Identity = 87/87 (100.00%), Postives = 87/87 (100.00%), Query Frame = -2 
Query:   79 MSALTDNQKEKLMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESSQYGA 339
            MSALTDNQKEKLMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESSQYGA
Sbjct:    1 MSALTDNQKEKLMEQVKAQAAIMQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTRLMKESSQYGA 87
[Close]
BLAST of SMED30024671 vs. Planmine SMEST
Match: SMESG000071085.1 (SMESG000071085.1)

HSP 1 Score: 41.5874 bits (96), Expect = 8.240e-6
Identity = 15/55 (27.27%), Postives = 30/55 (54.55%), Query Frame = -2 
Query:  109 MQIRDLMQTITDKCFDKCVTKIGTSLDSSQTKCAGQCMERYMEAHNLVTKTYSTR 273
            M++   +  +T KC+D+C  K+  SL +   KC   C++R++++   V +    R
Sbjct:   16 MELVSYINDLTKKCWDRCNIKVSASLATGDQKCIANCVDRFLDSSAFVARELPGR 70
The following BLAST results are available for this feature:
BLAST of SMED30024671 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
TIMM135.415e-2250.00translocase of inner mitochondrial membrane 13 [So... [more]
TIMM138.529e-1650.91translocase of inner mitochondrial membrane 13 [So... [more]
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BLAST of SMED30024671 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tin-133.580e-1741.03Mitochondrial import inner membrane translocase su... [more]
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BLAST of SMED30024671 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG341326.130e-2344.58gene:FBgn0083968 transcript:FBtr0110879[more]
Tim131.364e-2145.57gene:FBgn0036204 transcript:FBtr0076089[more]
CG423021.433e-729.03gene:FBgn0259198 transcript:FBtr0299682[more]
Tim86.515e-626.19gene:FBgn0027359 transcript:FBtr0343640[more]
Tim86.515e-626.19gene:FBgn0027359 transcript:FBtr0073482[more]
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BLAST of SMED30024671 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
timm135.936e-2247.95translocase of inner mitochondrial membrane 13 hom... [more]
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BLAST of SMED30024671 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
timm133.194e-2249.32translocase of inner mitochondrial membrane 13 hom... [more]
timm133.496e-1450.91translocase of inner mitochondrial membrane 13 hom... [more]
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BLAST of SMED30024671 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Timm133.334e-2249.32translocase of inner mitochondrial membrane 13 [So... [more]
Timm134.465e-1647.69translocase of inner mitochondrial membrane 13 [So... [more]
Timm133.816e-953.66translocase of inner mitochondrial membrane 13 [So... [more]
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BLAST of SMED30024671 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q8AVK1|TI13B_XENLA2.518e-2249.32Mitochondrial import inner membrane translocase su... [more]
sp|Q6GPY0|TI13A_XENLA1.620e-2149.32Mitochondrial import inner membrane translocase su... [more]
sp|P62076|TIM13_RAT2.332e-2149.32Mitochondrial import inner membrane translocase su... [more]
sp|P62075|TIM13_MOUSE2.332e-2149.32Mitochondrial import inner membrane translocase su... [more]
sp|Q9Y5L4|TIM13_HUMAN2.600e-2150.00Mitochondrial import inner membrane translocase su... [more]
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BLAST of SMED30024671 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5J4NSX83.578e-2651.19Mitochondrial import inner membrane translocase su... [more]
G7YIW84.458e-2550.60Mitochondrial import inner membrane translocase su... [more]
A0A1S8WUV25.545e-2548.81Tim10/DDP family zinc finger (Fragment) OS=Opistho... [more]
A0A4Z2CNF78.698e-2548.84Mitochondrial import inner membrane translocase su... [more]
A0A183VJZ49.359e-2547.67zf-Tim10_DDP domain-containing protein OS=Trichobi... [more]
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BLAST of SMED30024671 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
timm133.049e-2247.95translocase of inner mitochondrial membrane 13 [So... [more]
timm131.666e-851.22translocase of inner mitochondrial membrane 13 [So... [more]
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BLAST of SMED30024671 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024671 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
TIM131.065e-827.03Mitochondrial intermembrane space protein; forms a... [more]
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BLAST of SMED30024671 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3
Match NameE-valueIdentityDescription
EDO335276.313e-2250.70Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO444925.001e-729.49Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO262105.001e-729.49Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30024671 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
timm131.182e-2352.05translocase of inner mitochondrial membrane 13 [So... [more]
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BLAST of SMED30024671 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000017034.12.847e-61100.00SMESG000017034.1[more]
SMESG000017034.18.677e-60100.00SMESG000017034.1[more]
SMESG000071085.18.240e-627.27SMESG000071085.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024671 ID=SMED30024671|Name=SMED30024671|organism=Schmidtea mediterranea sexual|type=transcript|length=385bp
ATTTTATATTAGAAAACTAATTCAATGCGGATAACATGTGGAACATAAAT
TATAATATTTCCTATTTGTATGGACTTATGCGCCATATTGACTACTTTCT
TTCATTAACCGAGTACTGTAGGTTTTCGTGACTAAATTATGAGCTTCCAT
ATATCTTTCCATGCATTGACCGGCACATTTAGTTTGACTACTATCTAATG
ATGTTCCGATTTTTGTTACGCATTTATCGAAACATTTATCTGTTATTGTT
TGCATTAAATCTCTTATTTGCATTATTGCTGCTTGGGCTTTTACTTGTTC
CATAAGTTTTTCTTTTTGATTATCCGTTAAAGCTGACATATCCATTTTCA
CGCAAATTAATAAATTTTAAAATATTGTATTATTA
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000429neoblast
PLANA:0002032epidermal cell
PLANA:0002109X1 cell
PLANA:0002111X2 cell
PLANA:0002142parenchyma
Vocabulary: INTERPRO
TermDefinition
IPR035427Tim10-like_dom_sf
IPR039238Tim8/13
IPR004217Tim10-like
Vocabulary: cellular component
TermDefinition
GO:0005758mitochondrial intermembrane space
Vocabulary: biological process
TermDefinition
GO:0072321chaperone-mediated protein transport
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004217Tim10-likePFAMPF02953zf-Tim10_DDPcoord: 17..77
e-value: 4.9E-21
score: 74.1
IPR035427Tim10-like domain superfamilyGENE3DG3DSA:1.10.287.810coord: 23..88
e-value: 6.3E-20
score: 72.4
IPR035427Tim10-like domain superfamilySUPERFAMILYSSF144122Tim10-likecoord: 27..85
NoneNo IPR availablePANTHERPTHR19338:SF0MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM13coord: 3..85
IPR039238Mitochondrial import inner membrane translocase subunit Tim8/13PANTHERPTHR19338TRANSLOCASE OF INNER MITOCHONDRIAL MEMBRANE 13 HOMOLOGcoord: 3..85