Ligand of Numb protein X 2
|
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30024655 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7
Homology
BLAST of Ligand of Numb protein X 2 vs. Ensembl Human
Match: LNX2 (ligand of numb-protein X 2 [Source:HGNC Symbol;Acc:HGNC:20421]) HSP 1 Score: 266.159 bits (679), Expect = 2.353e-78 Identity = 181/508 (35.63%), Postives = 266/508 (52.36%), Query Frame = 3
Query: 585 ITIINPER----LEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVA---------KEEILTFVLEK-PTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDS--NMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKS-----TYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKV-------------PNLLLMANTD-NFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
IT I R ++ G+S VGG +TPL IVIQE++RDG IA DGRL GDQI ++N Y +S SH R VLS Q +T++LT+ R+ +R+ A +EEI L K + ++G+KL +T++ G++I ++L A+ DGRL +DR+L IN HDL +GT A +II++ + V I+R +PQ + IR AG S + TP P + P S K T +E+ + V K+P ESLGM+VAGG S+ G+ P+++T++ P+ L I +GD+LL +N +L LSH AV LK A V ++ L+V P+ D ++ PSW+ WL LP I L R + GS GFSIVGG + + TN +P IK+IV G+PA+ +GRLKCGD+++++N S +SHS V + K + V L V+ WPG+++
Sbjct: 231 ITTIEIHRSNPYIQLGISIVGGNETPLINIVIQEVYRDGVIARDGRLLAGDQILQVNNYNISNVSHNYARAVLS---QPCNTLHLTVLRE---RRFGNRAHNHSDSNSPREEIFQVALHKRDSGEQLGIKLVRRTDEPGVFILDLLEGGLAAQDGRLSSNDRVLAINGHDLKYGTPELAAQIIQASGERVNLTIARPGKPQPGNTIREAGNHSSSSQHHTP-----------------------PPYYSRPSSHKDLTQCVTCQEKHITVKKEPHESLGMTVAGGRGSKSGELPIFVTSVPPHGCLARDGRIKRGDVLLNINGIDLTNLSHSEAVAMLK-ASAASPAVALKALEVQIVEEATQNAEEQPSTFSENEYDASWSPSWVMWLGLPSTLHSCHDIVLRR-SYLGSWGFSIVGGYEENH---TN--------------QPFFIKTIVLGTPAYYDGRLKCGDMIVAVNGLSTVGMSHSALVPMLKEQRNKVTLTVICWPGSLV 690
BLAST of Ligand of Numb protein X 2 vs. Ensembl Human
Match: LNX2 (ligand of numb-protein X 2 [Source:HGNC Symbol;Acc:HGNC:20421]) HSP 1 Score: 266.159 bits (679), Expect = 2.353e-78 Identity = 181/508 (35.63%), Postives = 266/508 (52.36%), Query Frame = 3
Query: 585 ITIINPER----LEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVA---------KEEILTFVLEK-PTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDS--NMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKS-----TYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKV-------------PNLLLMANTD-NFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
IT I R ++ G+S VGG +TPL IVIQE++RDG IA DGRL GDQI ++N Y +S SH R VLS Q +T++LT+ R+ +R+ A +EEI L K + ++G+KL +T++ G++I ++L A+ DGRL +DR+L IN HDL +GT A +II++ + V I+R +PQ + IR AG S + TP P + P S K T +E+ + V K+P ESLGM+VAGG S+ G+ P+++T++ P+ L I +GD+LL +N +L LSH AV LK A V ++ L+V P+ D ++ PSW+ WL LP I L R + GS GFSIVGG + + TN +P IK+IV G+PA+ +GRLKCGD+++++N S +SHS V + K + V L V+ WPG+++
Sbjct: 231 ITTIEIHRSNPYIQLGISIVGGNETPLINIVIQEVYRDGVIARDGRLLAGDQILQVNNYNISNVSHNYARAVLS---QPCNTLHLTVLRE---RRFGNRAHNHSDSNSPREEIFQVALHKRDSGEQLGIKLVRRTDEPGVFILDLLEGGLAAQDGRLSSNDRVLAINGHDLKYGTPELAAQIIQASGERVNLTIARPGKPQPGNTIREAGNHSSSSQHHTP-----------------------PPYYSRPSSHKDLTQCVTCQEKHITVKKEPHESLGMTVAGGRGSKSGELPIFVTSVPPHGCLARDGRIKRGDVLLNINGIDLTNLSHSEAVAMLK-ASAASPAVALKALEVQIVEEATQNAEEQPSTFSENEYDASWSPSWVMWLGLPSTLHSCHDIVLRR-SYLGSWGFSIVGGYEENH---TN--------------QPFFIKTIVLGTPAYYDGRLKCGDMIVAVNGLSTVGMSHSALVPMLKEQRNKVTLTVICWPGSLV 690
BLAST of Ligand of Numb protein X 2 vs. Ensembl Human
Match: LNX1 (ligand of numb-protein X 1 [Source:HGNC Symbol;Acc:HGNC:6657]) HSP 1 Score: 253.062 bits (645), Expect = 6.572e-74 Identity = 180/541 (33.27%), Postives = 274/541 (50.65%), Query Frame = 3
Query: 462 ISNTNSGNNFPKPQDCFYQNNSFYTPLNHLDNLEFNVEDFTITIINPERLE----FGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRD----IVDKRWLPVA---KEEILTFVLEKPT-DRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKV-----------PNLL----LMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
+ T SG+ D +N+ T L + D IT I R++ + VGG +TPL I+IQ I+RDG IA DGRL PGD I K+N +S H +L + Q ++LT+ R+ + P A +++ +L K + + ++G+KL K ++ G++I VL A G+L+++DR+L IN HDL +G+ A +I++ + V V+SR VR ++PDI + AG + + +P PG E N+P P+ T E+VV + K PGESLGM+VAGG + + D P+Y+ ++ P ++ I GDILL V+ EL +S AV LK + + ++ L+V P L MA ++ PSW+ WL+LP + I L R+ A GSLGF IVGG + +N N +P IKSIV G+PA+ +GR++CGDIL+++N S S + H+ L K +G + L +VSWPGT +
Sbjct: 135 LRRTKSGSAVANHADQGRENSENTTAPEVFPRLYHLIPDGEITSIKINRVDPSESLSIRLVGGSETPLVHIIIQHIYRDGVIARDGRLLPGDIILKVNGMDISNVPHNYAVRLLRQPCQ---VLWLTVMREQKFRSRNNGQAPDAYRPRDDSFHVILNKSSPEEQLGIKLVRKVDEPGVFIFNVLDGGVAYRHGQLEENDRVLAINGHDLRYGSPESAAHLIQASERRVHLVVSRQVRQRSPDIFQEAGWNSNGSWSP------GPG---------ERSNTP----KPLHPTITCHEKVVNIQKDPGESLGMTVAGGASHREWDLPIYVISVEPGGVISRDGRIKTGDILLNVDGVELTEVSRSEAVALLK---RTSSSIVLKALEVKEYEPQEDCSSPAALDSNHNMAPPSDWSPSWVMWLELPRCLYNCKDIVLRRNTA-GSLGFCIVGGYEE---YNGN--------------KPFFIKSIVEGTPAYNDGRIRCGDILLAVNGRSTSGMIHACLARLLKELKGRITLTIVSWPGTFL 632
BLAST of Ligand of Numb protein X 2 vs. Ensembl Human
Match: LNX1 (ligand of numb-protein X 1 [Source:HGNC Symbol;Acc:HGNC:6657]) HSP 1 Score: 253.447 bits (646), Expect = 2.958e-73 Identity = 180/541 (33.27%), Postives = 274/541 (50.65%), Query Frame = 3
Query: 462 ISNTNSGNNFPKPQDCFYQNNSFYTPLNHLDNLEFNVEDFTITIINPERLE----FGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRD----IVDKRWLPVA---KEEILTFVLEKPT-DRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKV-----------PNLL----LMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
+ T SG+ D +N+ T L + D IT I R++ + VGG +TPL I+IQ I+RDG IA DGRL PGD I K+N +S H +L + Q ++LT+ R+ + P A +++ +L K + + ++G+KL K ++ G++I VL A G+L+++DR+L IN HDL +G+ A +I++ + V V+SR VR ++PDI + AG + + +P PG E N+P P+ T E+VV + K PGESLGM+VAGG + + D P+Y+ ++ P ++ I GDILL V+ EL +S AV LK + + ++ L+V P L MA ++ PSW+ WL+LP + I L R+ A GSLGF IVGG + +N N +P IKSIV G+PA+ +GR++CGDIL+++N S S + H+ L K +G + L +VSWPGT +
Sbjct: 231 LRRTKSGSAVANHADQGRENSENTTAPEVFPRLYHLIPDGEITSIKINRVDPSESLSIRLVGGSETPLVHIIIQHIYRDGVIARDGRLLPGDIILKVNGMDISNVPHNYAVRLLRQPCQ---VLWLTVMREQKFRSRNNGQAPDAYRPRDDSFHVILNKSSPEEQLGIKLVRKVDEPGVFIFNVLDGGVAYRHGQLEENDRVLAINGHDLRYGSPESAAHLIQASERRVHLVVSRQVRQRSPDIFQEAGWNSNGSWSP------GPG---------ERSNTP----KPLHPTITCHEKVVNIQKDPGESLGMTVAGGASHREWDLPIYVISVEPGGVISRDGRIKTGDILLNVDGVELTEVSRSEAVALLKRTS---SSIVLKALEVKEYEPQEDCSSPAALDSNHNMAPPSDWSPSWVMWLELPRCLYNCKDIVLRRNTA-GSLGFCIVGGYEE---YNGN--------------KPFFIKSIVEGTPAYNDGRIRCGDILLAVNGRSTSGMIHACLARLLKELKGRITLTIVSWPGTFL 728
BLAST of Ligand of Numb protein X 2 vs. Ensembl Human
Match: PATJ (PATJ crumbs cell polarity complex component [Source:HGNC Symbol;Acc:HGNC:28881]) HSP 1 Score: 186.808 bits (473), Expect = 9.793e-50 Identity = 150/483 (31.06%), Postives = 234/483 (48.45%), Query Frame = 3
Query: 585 ITIINPERLEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVAKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYP-------GLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNL-LLMANTDNFIPSWMYWLKLPGYCQI--------PRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVS 1985
I I+ R G+S VGG DTPL IVI E++ +G A DGRL GDQI ++N L +SH E ++ + Q+ + L ++RD + + EI L+K R +GL + GK N SG++I +++ A LDGRL + D+IL +N D+ +Q I+K + V+ I R+ R + R ++ A SS +P GL+N VG + S + + VE++++ ++LG+S+AGG S GD PV+I I + + T + GD ++++N L GLSH V LK A ++ ++V+ N+ + A +N S Y L P P++ITL + + LGFSIVGG S DL PI +K++ + A +GRLK GD ++++N +L V+H AV + K +G V L V+S
Sbjct: 221 IIEISKGRSGLGLSIVGGKDTPLNAIVIHEVYEEGAAARDGRLWAGDQILEVNGVDLRNSSHEEA---ITALRQTPQKVRLVVYRD--EAHYRDEENLEIFPVDLQKKAGRGLGLSIVGKRNGSGVFISDIVKGGAADLDGRLIQGDQILSVNGEDMRNASQETVATILKCAQGLVQLEIGRL-RAGSWTSARTT-SQNSQGSQQSAHSSCHPSFAPVITGLQNL------VGTKRVSDPSQKNSGTDMEPRTVEINRELSDALGISIAGGRGSPLGDIPVFIAMIQASGVAARTQKLKVGDRIVSINGQPLDGLSHADVVNLLKNA---YGRIILQVVADTNISAIAAQLENM--STGYHLGSPTAEHHPEDTETPPPKIITLEKGSE--GLGFSIVGG--------YGSPHGDL---------PIYVKTVFAKGAAADDGRLKRGDQILAVNGETLEGVTHEQAVAILKHQRGTVTLTVLS 666
BLAST of Ligand of Numb protein X 2 vs. Ensembl Celegans
Match: mpz-1 (Multiple PDZ domain protein [Source:UniProtKB/TrEMBL;Acc:G1K0W2]) HSP 1 Score: 106.301 bits (264), Expect = 6.157e-24 Identity = 75/257 (29.18%), Postives = 122/257 (47.47%), Query Frame = 3
Query: 615 FGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWL--PVAKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAA-------GTEPDSN------MTPIATSSSYPGLKNASTRGYEVGNSPFT-------FDSPISFKSTYREE 1319
G+S VGG DT L +VI E++ DG A DGRL+PGDQ+ ++N L +H + L R + L I+RD+ + L P I L K T R +G+ + G+ N+ G+Y+ E++ A DGRL D+IL++N D+ Q + ++K++ V + R +T + + AA T P + + P+A + S P +++ V ++P T PI ++ REE
Sbjct: 49 LGLSIVGGADTVLGTVVIHEVYSDGAAAHDGRLKPGDQVLEVNGTSLRGVTHDQSIAYLRRTPPK---VRLLIYRDVNLQLSLLDPTQIYNIFEIDLVKKTGRGLGISIVGRKNEPGVYVSEIVKGGLAESDGRLMTGDQILEVNGKDVRGCMQEDVAAMLKTITGKVHLKLGRWKITETANRVHAATQALAKSATTPRTTENNNDPVRPVAVAVSSPAATPSTSASVTVTSAPATSVPEPNVVVEPIPGPTSAREE 302 HSP 2 Score: 67.0106 bits (162), Expect = 2.379e-11 Identity = 65/239 (27.20%), Postives = 104/239 (43.51%), Query Frame = 3
Query: 1311 REEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVG-GNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII*TANQIQ 2024
RE ++E+DK G+ LG+S+ GG ++ G V I + + + + GD +L VN T L G++H++++ L R KV + + + NL L P+ +Y + I L + G LG SIVG N+P + + IV G A +GRL GD ++ +N + D + K+ G VHLK+ W I TAN++
Sbjct: 36 RETMIEIDKD-GKGLGLSIVGGADTVLGT--VVIHEVYSDGAAAHDGRLKPGDQVLEVNGTSLRGVTHDQSIAYL---RRTPPKVRLLIYRDVNLQLSL----LDPTQIYNI---------FEIDLVKKTGRG-LGISIVGRKNEP----------------------GVYVSEIVKGGLAESDGRLMTGDQILEVNGKDVRGCMQEDVAAMLKTITGKVHLKLGRW--KITETANRVH 230
BLAST of Ligand of Numb protein X 2 vs. Ensembl Celegans
Match: mpz-1 (Multiple PDZ domain protein [Source:UniProtKB/TrEMBL;Acc:G1K0W2]) HSP 1 Score: 105.145 bits (261), Expect = 4.964e-23 Identity = 73/257 (28.40%), Postives = 125/257 (48.64%), Query Frame = 3
Query: 615 FGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWL--PVAKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAA-------GTEPDSN------MTPIATSSSYPGLKNASTRGYEVGNSPFT-------FDSPISFKSTYREE 1319
G+S VGG DT L +VI E++ DG A DGRL+PGDQ+ ++N L +H + ++ + ++ + L I+RD+ + L P I L K T R +G+ + G+ N+ G+Y+ E++ A DGRL D+IL++N D+ Q + ++K++ V + R +T + + AA T P + + P+A + S P +++ V ++P T PI ++ REE
Sbjct: 559 LGLSIVGGADTVLGTVVIHEVYSDGAAAHDGRLKPGDQVLEVNGTSLRGVTHDQS---IAYLRRTPPKVRLLIYRDVNLQLSLLDPTQIYNIFEIDLVKKTGRGLGISIVGRKNEPGVYVSEIVKGGLAESDGRLMTGDQILEVNGKDVRGCMQEDVAAMLKTITGKVHLKLGRWKITETANRVHAATQALAKSATTPRTTENNNDPVRPVAVAVSSPAATPSTSASVTVTSAPATSVPEPNVVVEPIPGPTSAREE 812 HSP 2 Score: 67.3958 bits (163), Expect = 3.070e-11 Identity = 65/239 (27.20%), Postives = 104/239 (43.51%), Query Frame = 3
Query: 1311 REEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVG-GNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII*TANQIQ 2024
RE ++E+DK G+ LG+S+ GG ++ G V I + + + + GD +L VN T L G++H++++ L R KV + + + NL L P+ +Y + I L + G LG SIVG N+P + + IV G A +GRL GD ++ +N + D + K+ G VHLK+ W I TAN++
Sbjct: 546 RETMIEIDKD-GKGLGLSIVGGADTVLGT--VVIHEVYSDGAAAHDGRLKPGDQVLEVNGTSLRGVTHDQSIAYL---RRTPPKVRLLIYRDVNLQLSL----LDPTQIYNI---------FEIDLVKKTGRG-LGISIVGRKNEP----------------------GVYVSEIVKGGLAESDGRLMTGDQILEVNGKDVRGCMQEDVAAMLKTITGKVHLKLGRW--KITETANRVH 740 HSP 3 Score: 52.7582 bits (125), Expect = 8.488e-7 Identity = 37/117 (31.62%), Postives = 53/117 (45.30%), Query Frame = 3
Query: 1638 YWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPI-----YPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHL 1973
YW + R +TL R+ S G SIVGG + P N+ I IKS++ SPA ++G++ GD +IS+ND L + +H AVN K+ PV
Sbjct: 28 YWGEA-------RTVTLVREP-NKSFGISIVGGRVEVSQKGGLPGTGNTVCG------------IFIKSVLPNSPAGRSGQMNMGDRVISVNDVDLRDATHEQAVNAIKNASNPVRF 124
BLAST of Ligand of Numb protein X 2 vs. Ensembl Celegans
Match: mpz-1 (Multiple PDZ domain protein [Source:UniProtKB/TrEMBL;Acc:G1K0W2]) HSP 1 Score: 105.531 bits (262), Expect = 5.242e-23 Identity = 75/257 (29.18%), Postives = 123/257 (47.86%), Query Frame = 3
Query: 615 FGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWL--PVAKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAA-------GTEPDSN------MTPIATSSSYPGLKNASTRGYEVGNSPFT-------FDSPISFKSTYREE 1319
G+S VGG DT L +VI E++ DG A DGRL+PGDQ+ ++N L +H + L R + + L I+RD+ + L P I L K T R +G+ + G+ N+ G+Y+ E++ A DGRL D+IL++N D+ Q + ++K++ V + R +T + + AA T P + + P+A + S P +++ V ++P T PI ++ REE
Sbjct: 1763 LGLSIVGGADTVLGTVVIHEVYSDGAAAHDGRLKPGDQVLEVNGTSLRGVTHDQSIAYLRR---TPPKVRLLIYRDVNLQLSLLDPTQIYNIFEIDLVKKTGRGLGISIVGRKNEPGVYVSEIVKGGLAESDGRLMTGDQILEVNGKDVRGCMQEDVAAMLKTITGKVHLKLGRWKITETANRVHAATQALAKSATTPRTTENNNDPVRPVAVAVSSPAATPSTSASVTVTSAPATSVPEPNVVVEPIPGPTSAREE 2016 HSP 2 Score: 67.3958 bits (163), Expect = 3.348e-11 Identity = 65/239 (27.20%), Postives = 104/239 (43.51%), Query Frame = 3
Query: 1311 REEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVG-GNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII*TANQIQ 2024
RE ++E+DK G+ LG+S+ GG ++ G V I + + + + GD +L VN T L G++H++++ L R KV + + + NL L P+ +Y + I L + G LG SIVG N+P + + IV G A +GRL GD ++ +N + D + K+ G VHLK+ W I TAN++
Sbjct: 1750 RETMIEIDKD-GKGLGLSIVGGADTVLGT--VVIHEVYSDGAAAHDGRLKPGDQVLEVNGTSLRGVTHDQSIAYL---RRTPPKVRLLIYRDVNLQLSL----LDPTQIYNI---------FEIDLVKKTGRG-LGISIVGRKNEP----------------------GVYVSEIVKGGLAESDGRLMTGDQILEVNGKDVRGCMQEDVAAMLKTITGKVHLKLGRW--KITETANRVH 1944 HSP 3 Score: 53.1434 bits (126), Expect = 7.461e-7 Identity = 43/146 (29.45%), Postives = 63/146 (43.15%), Query Frame = 3
Query: 1551 NCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPI-----YPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHL 1973
N ++VT R + L + S YW + R +TL R+ S G SIVGG + P N+ I IKS++ SPA ++G++ GD +IS+ND L + +H AVN K+ PV
Sbjct: 1204 NMSRVTSRTPSTGSESLQNQARQLVRS-KYWGEA-------RTVTLVREP-NKSFGISIVGGRVEVSQKGGLPGTGNTVCG------------IFIKSVLPNSPAGRSGQMNMGDRVISVNDVDLRDATHEQAVNAIKNASNPVRF 1328
BLAST of Ligand of Numb protein X 2 vs. Ensembl Celegans
Match: mpz-1 (Multiple PDZ domain protein [Source:UniProtKB/TrEMBL;Acc:G1K0W2]) HSP 1 Score: 105.531 bits (262), Expect = 5.391e-23 Identity = 75/257 (29.18%), Postives = 123/257 (47.86%), Query Frame = 3
Query: 615 FGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWL--PVAKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAA-------GTEPDSN------MTPIATSSSYPGLKNASTRGYEVGNSPFT-------FDSPISFKSTYREE 1319
G+S VGG DT L +VI E++ DG A DGRL+PGDQ+ ++N L +H + L R + + L I+RD+ + L P I L K T R +G+ + G+ N+ G+Y+ E++ A DGRL D+IL++N D+ Q + ++K++ V + R +T + + AA T P + + P+A + S P +++ V ++P T PI ++ REE
Sbjct: 988 LGLSIVGGADTVLGTVVIHEVYSDGAAAHDGRLKPGDQVLEVNGTSLRGVTHDQSIAYLRR---TPPKVRLLIYRDVNLQLSLLDPTQIYNIFEIDLVKKTGRGLGISIVGRKNEPGVYVSEIVKGGLAESDGRLMTGDQILEVNGKDVRGCMQEDVAAMLKTITGKVHLKLGRWKITETANRVHAATQALAKSATTPRTTENNNDPVRPVAVAVSSPAATPSTSASVTVTSAPATSVPEPNVVVEPIPGPTSAREE 1241 HSP 2 Score: 67.3958 bits (163), Expect = 3.196e-11 Identity = 65/239 (27.20%), Postives = 104/239 (43.51%), Query Frame = 3
Query: 1311 REEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVG-GNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII*TANQIQ 2024
RE ++E+DK G+ LG+S+ GG ++ G V I + + + + GD +L VN T L G++H++++ L R KV + + + NL L P+ +Y + I L + G LG SIVG N+P + + IV G A +GRL GD ++ +N + D + K+ G VHLK+ W I TAN++
Sbjct: 975 RETMIEIDKD-GKGLGLSIVGGADTVLGT--VVIHEVYSDGAAAHDGRLKPGDQVLEVNGTSLRGVTHDQSIAYL---RRTPPKVRLLIYRDVNLQLSL----LDPTQIYNI---------FEIDLVKKTGRG-LGISIVGRKNEP----------------------GVYVSEIVKGGLAESDGRLMTGDQILEVNGKDVRGCMQEDVAAMLKTITGKVHLKLGRW--KITETANRVH 1169 HSP 3 Score: 53.9138 bits (128), Expect = 4.083e-7 Identity = 43/146 (29.45%), Postives = 63/146 (43.15%), Query Frame = 3
Query: 1551 NCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPI-----YPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHL 1973
N ++VT R + L + S YW + R +TL R+ S G SIVGG + P N+ I IKS++ SPA ++G++ GD +IS+ND L + +H AVN K+ PV
Sbjct: 429 NMSRVTSRTPSTGSESLQNQARQLVRS-KYWGEA-------RTVTLVREP-NKSFGISIVGGRVEVSQKGGLPGTGNTVCG------------IFIKSVLPNSPAGRSGQMNMGDRVISVNDVDLRDATHEQAVNAIKNASNPVRF 553
BLAST of Ligand of Numb protein X 2 vs. Ensembl Celegans
Match: mpz-1 (Multiple PDZ domain protein [Source:UniProtKB/TrEMBL;Acc:G1K0W2]) HSP 1 Score: 105.531 bits (262), Expect = 5.484e-23 Identity = 75/257 (29.18%), Postives = 123/257 (47.86%), Query Frame = 3
Query: 615 FGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWL--PVAKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAA-------GTEPDSN------MTPIATSSSYPGLKNASTRGYEVGNSPFT-------FDSPISFKSTYREE 1319
G+S VGG DT L +VI E++ DG A DGRL+PGDQ+ ++N L +H + L R + + L I+RD+ + L P I L K T R +G+ + G+ N+ G+Y+ E++ A DGRL D+IL++N D+ Q + ++K++ V + R +T + + AA T P + + P+A + S P +++ V ++P T PI ++ REE
Sbjct: 1781 LGLSIVGGADTVLGTVVIHEVYSDGAAAHDGRLKPGDQVLEVNGTSLRGVTHDQSIAYLRR---TPPKVRLLIYRDVNLQLSLLDPTQIYNIFEIDLVKKTGRGLGISIVGRKNEPGVYVSEIVKGGLAESDGRLMTGDQILEVNGKDVRGCMQEDVAAMLKTITGKVHLKLGRWKITETANRVHAATQALAKSATTPRTTENNNDPVRPVAVAVSSPAATPSTSASVTVTSAPATSVPEPNVVVEPIPGPTSAREE 2034 HSP 2 Score: 67.3958 bits (163), Expect = 3.527e-11 Identity = 65/239 (27.20%), Postives = 104/239 (43.51%), Query Frame = 3
Query: 1311 REEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVG-GNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII*TANQIQ 2024
RE ++E+DK G+ LG+S+ GG ++ G V I + + + + GD +L VN T L G++H++++ L R KV + + + NL L P+ +Y + I L + G LG SIVG N+P + + IV G A +GRL GD ++ +N + D + K+ G VHLK+ W I TAN++
Sbjct: 1768 RETMIEIDKD-GKGLGLSIVGGADTVLGT--VVIHEVYSDGAAAHDGRLKPGDQVLEVNGTSLRGVTHDQSIAYL---RRTPPKVRLLIYRDVNLQLSL----LDPTQIYNI---------FEIDLVKKTGRG-LGISIVGRKNEP----------------------GVYVSEIVKGGLAESDGRLMTGDQILEVNGKDVRGCMQEDVAAMLKTITGKVHLKLGRW--KITETANRVH 1962 HSP 3 Score: 53.1434 bits (126), Expect = 7.660e-7 Identity = 43/146 (29.45%), Postives = 63/146 (43.15%), Query Frame = 3
Query: 1551 NCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPI-----YPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHL 1973
N ++VT R + L + S YW + R +TL R+ S G SIVGG + P N+ I IKS++ SPA ++G++ GD +IS+ND L + +H AVN K+ PV
Sbjct: 1222 NMSRVTSRTPSTGSESLQNQARQLVRS-KYWGEA-------RTVTLVREP-NKSFGISIVGGRVEVSQKGGLPGTGNTVCG------------IFIKSVLPNSPAGRSGQMNMGDRVISVNDVDLRDATHEQAVNAIKNASNPVRF 1346
BLAST of Ligand of Numb protein X 2 vs. Ensembl Fly
Match: scrib (gene:FBgn0263289 transcript:FBtr0308623) HSP 1 Score: 75.485 bits (184), Expect = 1.522e-13 Identity = 53/189 (28.04%), Postives = 92/189 (48.68%), Query Frame = 3
Query: 615 FGVSFVGG-------FDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVAKEEILTFVLEKPTDRKMGLKLAGKTN----------KSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISR 1130
G S +GG F T I I I G + G+LR GD+I K+NE +S+A+H +D + + + GD I LTI D LP +E+L L K ++G+ + G N G+++ ++ A DGRLK R+L++N H L+ + +A+ ++++ + ++ V+ +
Sbjct: 1249 LGFSIIGGTDHSCVPFGTREPGIFISHIVPGGIASKCGKLRMGDRILKVNEADVSKATH---QDAVLELLKPGDEIKLTIQHD-----PLPPGFQEVL---LSKAEGERLGMHIKGGLNGQRGNPADPSDEGVFVSKINSVGAARRDGRLKVGMRLLEVNGHSLLGASHQDAVNVLRNAGNEIQLVVCK 1426 HSP 2 Score: 62.3882 bits (150), Expect = 1.340e-9 Identity = 78/296 (26.35%), Postives = 114/296 (38.51%), Query Frame = 3
Query: 762 ASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWL-PVAKEEILTFVLEKPTDRKMGLKLAGKTNKS---------GLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEA-LEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRG------DTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLL 1598
A+ E V I++S T T+ I D + P+ EE+ VL K +G + G T+ S G++I ++P AS G+L+ DRILK+NE D+ T +A LE++K D +K I P PG + V + K GE LGM + GG+N QRG D V+++ I + + G LL VN LLG SH+ AV L+ A + V + NL+
Sbjct: 1201 AAPTELGRVTETITKS--TFTETVMTRITDNQLAEPLISEEV---VLPK-NQGSLGFSIIGGTDHSCVPFGTREPGIFISHIVPGGIASKCGKLRMGDRILKVNEADVSKATHQDAVLELLKP-GDEIKLTIQHDPLP--------------------------PGFQE-----------------------------VLLSKAEGERLGMHIKGGLNGQRGNPADPSDEGVFVSKINSVGAARRDGRLKVGMRLLEVNGHSLLGASHQDAVNVLRNAGNEIQLVVCKGYDKSNLI 1434
BLAST of Ligand of Numb protein X 2 vs. Ensembl Fly
Match: scrib (gene:FBgn0263289 transcript:FBtr0308632) HSP 1 Score: 75.0998 bits (183), Expect = 1.661e-13 Identity = 53/189 (28.04%), Postives = 92/189 (48.68%), Query Frame = 3
Query: 615 FGVSFVGG-------FDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVAKEEILTFVLEKPTDRKMGLKLAGKTN----------KSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISR 1130
G S +GG F T I I I G + G+LR GD+I K+NE +S+A+H +D + + + GD I LTI D LP +E+L L K ++G+ + G N G+++ ++ A DGRLK R+L++N H L+ + +A+ ++++ + ++ V+ +
Sbjct: 1274 LGFSIIGGTDHSCVPFGTREPGIFISHIVPGGIASKCGKLRMGDRILKVNEADVSKATH---QDAVLELLKPGDEIKLTIQHD-----PLPPGFQEVL---LSKAEGERLGMHIKGGLNGQRGNPADPSDEGVFVSKINSVGAARRDGRLKVGMRLLEVNGHSLLGASHQDAVNVLRNAGNEIQLVVCK 1451 HSP 2 Score: 62.7734 bits (151), Expect = 1.076e-9 Identity = 78/296 (26.35%), Postives = 114/296 (38.51%), Query Frame = 3
Query: 762 ASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWL-PVAKEEILTFVLEKPTDRKMGLKLAGKTNKS---------GLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEA-LEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRG------DTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLL 1598
A+ E V I++S T T+ I D + P+ EE+ VL K +G + G T+ S G++I ++P AS G+L+ DRILK+NE D+ T +A LE++K D +K I P PG + V + K GE LGM + GG+N QRG D V+++ I + + G LL VN LLG SH+ AV L+ A + V + NL+
Sbjct: 1226 AAPTELGRVTETITKS--TFTETVMTRITDNQLAEPLISEEV---VLPK-NQGSLGFSIIGGTDHSCVPFGTREPGIFISHIVPGGIASKCGKLRMGDRILKVNEADVSKATHQDAVLELLKP-GDEIKLTIQHDPLP--------------------------PGFQE-----------------------------VLLSKAEGERLGMHIKGGLNGQRGNPADPSDEGVFVSKINSVGAARRDGRLKVGMRLLEVNGHSLLGASHQDAVNVLRNAGNEIQLVVCKGYDKSNLI 1459
BLAST of Ligand of Numb protein X 2 vs. Ensembl Fly
Match: scrib (gene:FBgn0263289 transcript:FBtr0308630) HSP 1 Score: 74.7146 bits (182), Expect = 2.331e-13 Identity = 53/189 (28.04%), Postives = 92/189 (48.68%), Query Frame = 3
Query: 615 FGVSFVGG-------FDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVAKEEILTFVLEKPTDRKMGLKLAGKTN----------KSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISR 1130
G S +GG F T I I I G + G+LR GD+I K+NE +S+A+H +D + + + GD I LTI D LP +E+L L K ++G+ + G N G+++ ++ A DGRLK R+L++N H L+ + +A+ ++++ + ++ V+ +
Sbjct: 1274 LGFSIIGGTDHSCVPFGTREPGIFISHIVPGGIASKCGKLRMGDRILKVNEADVSKATH---QDAVLELLKPGDEIKLTIQHD-----PLPPGFQEVL---LSKAEGERLGMHIKGGLNGQRGNPADPSDEGVFVSKINSVGAARRDGRLKVGMRLLEVNGHSLLGASHQDAVNVLRNAGNEIQLVVCK 1451 HSP 2 Score: 62.7734 bits (151), Expect = 1.125e-9 Identity = 78/296 (26.35%), Postives = 114/296 (38.51%), Query Frame = 3
Query: 762 ASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWL-PVAKEEILTFVLEKPTDRKMGLKLAGKTNKS---------GLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEA-LEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRG------DTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLL 1598
A+ E V I++S T T+ I D + P+ EE+ VL K +G + G T+ S G++I ++P AS G+L+ DRILK+NE D+ T +A LE++K D +K I P PG + V + K GE LGM + GG+N QRG D V+++ I + + G LL VN LLG SH+ AV L+ A + V + NL+
Sbjct: 1226 AAPTELGRVTETITKS--TFTETVMTRITDNQLAEPLISEEV---VLPK-NQGSLGFSIIGGTDHSCVPFGTREPGIFISHIVPGGIASKCGKLRMGDRILKVNEADVSKATHQDAVLELLKP-GDEIKLTIQHDPLP--------------------------PGFQE-----------------------------VLLSKAEGERLGMHIKGGLNGQRGNPADPSDEGVFVSKINSVGAARRDGRLKVGMRLLEVNGHSLLGASHQDAVNVLRNAGNEIQLVVCKGYDKSNLI 1459
BLAST of Ligand of Numb protein X 2 vs. Ensembl Fly
Match: scrib (gene:FBgn0263289 transcript:FBtr0308622) HSP 1 Score: 74.7146 bits (182), Expect = 2.369e-13 Identity = 53/189 (28.04%), Postives = 92/189 (48.68%), Query Frame = 3
Query: 615 FGVSFVGG-------FDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVAKEEILTFVLEKPTDRKMGLKLAGKTN----------KSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISR 1130
G S +GG F T I I I G + G+LR GD+I K+NE +S+A+H +D + + + GD I LTI D LP +E+L L K ++G+ + G N G+++ ++ A DGRLK R+L++N H L+ + +A+ ++++ + ++ V+ +
Sbjct: 1337 LGFSIIGGTDHSCVPFGTREPGIFISHIVPGGIASKCGKLRMGDRILKVNEADVSKATH---QDAVLELLKPGDEIKLTIQHD-----PLPPGFQEVL---LSKAEGERLGMHIKGGLNGQRGNPADPSDEGVFVSKINSVGAARRDGRLKVGMRLLEVNGHSLLGASHQDAVNVLRNAGNEIQLVVCK 1514 HSP 2 Score: 60.8474 bits (146), Expect = 3.991e-9 Identity = 60/225 (26.67%), Postives = 87/225 (38.67%), Query Frame = 3
Query: 945 TNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEA-LEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRG------DTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLL 1598
T + G++I ++P AS G+L+ DRILK+NE D+ T +A LE++K D +K I P PG + V + K GE LGM + GG+N QRG D V+++ I + + G LL VN LLG SH+ AV L+ A + V + NL+
Sbjct: 1354 TREPGIFISHIVPGGIASKCGKLRMGDRILKVNEADVSKATHQDAVLELLKP-GDEIKLTIQHDPLP--------------------------PGFQE-----------------------------VLLSKAEGERLGMHIKGGLNGQRGNPADPSDEGVFVSKINSVGAARRDGRLKVGMRLLEVNGHSLLGASHQDAVNVLRNAGNEIQLVVCKGYDKSNLI 1522
BLAST of Ligand of Numb protein X 2 vs. Ensembl Fly
Match: scrib (gene:FBgn0263289 transcript:FBtr0308625) HSP 1 Score: 74.7146 bits (182), Expect = 2.493e-13 Identity = 53/189 (28.04%), Postives = 92/189 (48.68%), Query Frame = 3
Query: 615 FGVSFVGG-------FDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVAKEEILTFVLEKPTDRKMGLKLAGKTN----------KSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISR 1130
G S +GG F T I I I G + G+LR GD+I K+NE +S+A+H +D + + + GD I LTI D LP +E+L L K ++G+ + G N G+++ ++ A DGRLK R+L++N H L+ + +A+ ++++ + ++ V+ +
Sbjct: 1249 LGFSIIGGTDHSCVPFGTREPGIFISHIVPGGIASKCGKLRMGDRILKVNEADVSKATH---QDAVLELLKPGDEIKLTIQHD-----PLPPGFQEVL---LSKAEGERLGMHIKGGLNGQRGNPADPSDEGVFVSKINSVGAARRDGRLKVGMRLLEVNGHSLLGASHQDAVNVLRNAGNEIQLVVCK 1426 HSP 2 Score: 62.003 bits (149), Expect = 1.957e-9 Identity = 78/296 (26.35%), Postives = 114/296 (38.51%), Query Frame = 3
Query: 762 ASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWL-PVAKEEILTFVLEKPTDRKMGLKLAGKTNKS---------GLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEA-LEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRG------DTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLL 1598
A+ E V I++S T T+ I D + P+ EE+ VL K +G + G T+ S G++I ++P AS G+L+ DRILK+NE D+ T +A LE++K D +K I P PG + V + K GE LGM + GG+N QRG D V+++ I + + G LL VN LLG SH+ AV L+ A + V + NL+
Sbjct: 1201 AAPTELGRVTETITKS--TFTETVMTRITDNQLAEPLISEEV---VLPK-NQGSLGFSIIGGTDHSCVPFGTREPGIFISHIVPGGIASKCGKLRMGDRILKVNEADVSKATHQDAVLELLKP-GDEIKLTIQHDPLP--------------------------PGFQE-----------------------------VLLSKAEGERLGMHIKGGLNGQRGNPADPSDEGVFVSKINSVGAARRDGRLKVGMRLLEVNGHSLLGASHQDAVNVLRNAGNEIQLVVCKGYDKSNLI 1434
BLAST of Ligand of Numb protein X 2 vs. Ensembl Zebrafish
Match: lnx2a (ligand of numb-protein X 2a [Source:ZFIN;Acc:ZDB-GENE-060228-2]) HSP 1 Score: 256.529 bits (654), Expect = 9.068e-75 Identity = 174/490 (35.51%), Postives = 266/490 (54.29%), Query Frame = 3
Query: 612 EFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVAKEE-------ILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPD----SNMTPI---ATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKV-----PNL---LLMANTDNF----IPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
E G+S VGG +TPL +VIQE++RDG IA DGRL GDQI ++N +S H R L+R T+ LT+ R+ P A I + ++ + ++G+KL +T+++G++I ++L A+ DGRL +DR+L +NEHDL GT A +II++ + V +ISR + QT + + D ++ P+ AT S P L A + + +S +E+ + V K+P ESLGM+VAGG S+ G+ P+++T++ P+ L I +GD+LL++N +L LSH AV LK + +C+ V ++ L+V P L LL + +++ PSW+ WL LP Y I L R + GS GFSIVGG Y N H+ +A IK+IV G+PA+ +GRLKCGD+++++N S + +SHS V + K + V L VVSWPG++I
Sbjct: 249 EMGISIVGGNETPLINVVIQEVYRDGVIARDGRLLAGDQILQVNNVDISNVPHNFARSTLARPCA---TLQLTVLRERRCSARPPAATASPKGSPASIRITLHKRESSEQLGIKLVRRTDEAGVFILDLLEGGLAAKDGRLCSNDRVLAVNEHDLRHGTPELAAQIIQASGERVNLLISRSSK-QTMAVHTGSTLTRDIWSHDHIPPLPSTATPSPVPSLHLARS----------STQRDLSQCVNCKEKHITVKKEPHESLGMTVAGGRGSKSGELPIFVTSVQPHGCLSRDGRIKRGDVLLSINGQDLTYLSHSEAVGTLKSSATSCS-VQLKALEVTMVEEPGLDEELLPPHENDYDASWSPSWVLWLGLPSYLHSSHEIVLRR-SHPGSWGFSIVGG----YEEN---------HSNQA----FFIKTIVLGTPAYYDGRLKCGDMIVAVNGLSTAGMSHSALVPMLKEQRSRVALTVVSWPGSLI 705
BLAST of Ligand of Numb protein X 2 vs. Ensembl Zebrafish
Match: lnx2a (ligand of numb-protein X 2a [Source:ZFIN;Acc:ZDB-GENE-060228-2]) HSP 1 Score: 256.529 bits (654), Expect = 1.621e-74 Identity = 174/490 (35.51%), Postives = 266/490 (54.29%), Query Frame = 3
Query: 612 EFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVAKEE-------ILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPD----SNMTPI---ATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKV-----PNL---LLMANTDNF----IPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
E G+S VGG +TPL +VIQE++RDG IA DGRL GDQI ++N +S H R L+R T+ LT+ R+ P A I + ++ + ++G+KL +T+++G++I ++L A+ DGRL +DR+L +NEHDL GT A +II++ + V +ISR + QT + + D ++ P+ AT S P L A + + +S +E+ + V K+P ESLGM+VAGG S+ G+ P+++T++ P+ L I +GD+LL++N +L LSH AV LK + +C+ V ++ L+V P L LL + +++ PSW+ WL LP Y I L R + GS GFSIVGG Y N H+ +A IK+IV G+PA+ +GRLKCGD+++++N S + +SHS V + K + V L VVSWPG++I
Sbjct: 281 EMGISIVGGNETPLINVVIQEVYRDGVIARDGRLLAGDQILQVNNVDISNVPHNFARSTLARPCA---TLQLTVLRERRCSARPPAATASPKGSPASIRITLHKRESSEQLGIKLVRRTDEAGVFILDLLEGGLAAKDGRLCSNDRVLAVNEHDLRHGTPELAAQIIQASGERVNLLISRSSK-QTMAVHTGSTLTRDIWSHDHIPPLPSTATPSPVPSLHLARS----------STQRDLSQCVNCKEKHITVKKEPHESLGMTVAGGRGSKSGELPIFVTSVQPHGCLSRDGRIKRGDVLLSINGQDLTYLSHSEAVGTLKSSATSCS-VQLKALEVTMVEEPGLDEELLPPHENDYDASWSPSWVLWLGLPSYLHSSHEIVLRR-SHPGSWGFSIVGG----YEEN---------HSNQA----FFIKTIVLGTPAYYDGRLKCGDMIVAVNGLSTAGMSHSALVPMLKEQRSRVALTVVSWPGSLI 737
BLAST of Ligand of Numb protein X 2 vs. Ensembl Zebrafish
Match: lnx2a (ligand of numb-protein X 2a [Source:ZFIN;Acc:ZDB-GENE-060228-2]) HSP 1 Score: 256.529 bits (654), Expect = 1.621e-74 Identity = 174/490 (35.51%), Postives = 266/490 (54.29%), Query Frame = 3
Query: 612 EFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVAKEE-------ILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPD----SNMTPI---ATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKV-----PNL---LLMANTDNF----IPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
E G+S VGG +TPL +VIQE++RDG IA DGRL GDQI ++N +S H R L+R T+ LT+ R+ P A I + ++ + ++G+KL +T+++G++I ++L A+ DGRL +DR+L +NEHDL GT A +II++ + V +ISR + QT + + D ++ P+ AT S P L A + + +S +E+ + V K+P ESLGM+VAGG S+ G+ P+++T++ P+ L I +GD+LL++N +L LSH AV LK + +C+ V ++ L+V P L LL + +++ PSW+ WL LP Y I L R + GS GFSIVGG Y N H+ +A IK+IV G+PA+ +GRLKCGD+++++N S + +SHS V + K + V L VVSWPG++I
Sbjct: 281 EMGISIVGGNETPLINVVIQEVYRDGVIARDGRLLAGDQILQVNNVDISNVPHNFARSTLARPCA---TLQLTVLRERRCSARPPAATASPKGSPASIRITLHKRESSEQLGIKLVRRTDEAGVFILDLLEGGLAAKDGRLCSNDRVLAVNEHDLRHGTPELAAQIIQASGERVNLLISRSSK-QTMAVHTGSTLTRDIWSHDHIPPLPSTATPSPVPSLHLARS----------STQRDLSQCVNCKEKHITVKKEPHESLGMTVAGGRGSKSGELPIFVTSVQPHGCLSRDGRIKRGDVLLSINGQDLTYLSHSEAVGTLKSSATSCS-VQLKALEVTMVEEPGLDEELLPPHENDYDASWSPSWVLWLGLPSYLHSSHEIVLRR-SHPGSWGFSIVGG----YEEN---------HSNQA----FFIKTIVLGTPAYYDGRLKCGDMIVAVNGLSTAGMSHSALVPMLKEQRSRVALTVVSWPGSLI 737
BLAST of Ligand of Numb protein X 2 vs. Ensembl Zebrafish
Match: lnx1 (ligand of numb-protein X 1 [Source:ZFIN;Acc:ZDB-GENE-030131-9439]) HSP 1 Score: 245.358 bits (625), Expect = 2.687e-70 Identity = 169/530 (31.89%), Postives = 266/530 (50.19%), Query Frame = 3
Query: 549 LDNLEFNVEDFTITIINPERLE----FGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDK--------------RWLPVAKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAA--GTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKV--PN-----------------------LLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
L L + D +T I R + +S VGG +TPL I+IQ+I+R+G IA DGRL PGD I K+N +S H C V + + Q + LT+ R+ + + + + ++++ D ++G+KL + ++ G++I +L A+ DGRL+ DDR+L IN HDL +G A +I++ +D V F++SR PDI++ A E +P+ + DS K E+ V + K+P +SLGM+VAGG++S+ D PVY+TN+ PN ++ I KGDILL VN +L G++ AV LK + + V ++VL++ PN + L D++ P W+ WL+LP + + I L R + +GSLGFSIVGG + + N N S I+SIV G+PA+ +GR++CGDIL+ +N S ++H+ V L K +G + L +VSWPG+++
Sbjct: 270 LPQLHHLIPDGEVTSIKITRADPCEPLAISIVGGNETPLVRILIQDIYREGVIARDGRLLPGDMILKVNGIDISNVPH--CYAVAA-LKQPCTLLRLTVLREQRHRYRSHHHSPTEAFPAHTATIRDDSLHVVLVKRAPDEQLGIKLVRRPDEHGVFIFHLLEGGLAARDGRLRVDDRVLAINGHDLRYGAPEHAALLIQASEDRVHFIVSRQTHIPAPDILQEAPWNMEGPPPYSPVDIEHTL-------------------LDS--CQKPACYEKTVTLLKEPHDSLGMTVAGGMSSRGWDLPVYVTNVDPNGVVGQEGSIRKGDILLNVNGVDLTGVTRSEAVANLK---NTSSPVVLQVLEMRPPNESSLDCMPPLHSPCALSPSSPGDVKLPPPNDDYAPLWVSWLQLPRHLYCCKDIVLRR-STSGSLGFSIVGGQEEL---NCNQSF--------------FIRSIVEGTPAYNDGRIRCGDILLEVNGKSTWGMTHTALVRLLKELRGRITLTIVSWPGSLL 754
BLAST of Ligand of Numb protein X 2 vs. Ensembl Zebrafish
Match: lnx1 (ligand of numb-protein X 1 [Source:ZFIN;Acc:ZDB-GENE-030131-9439]) HSP 1 Score: 244.973 bits (624), Expect = 4.444e-70 Identity = 169/530 (31.89%), Postives = 266/530 (50.19%), Query Frame = 3
Query: 549 LDNLEFNVEDFTITIINPERLE----FGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDK--------------RWLPVAKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAA--GTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKV--PN-----------------------LLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
L L + D +T I R + +S VGG +TPL I+IQ+I+R+G IA DGRL PGD I K+N +S H C V + + Q + LT+ R+ + + + + ++++ D ++G+KL + ++ G++I +L A+ DGRL+ DDR+L IN HDL +G A +I++ +D V F++SR PDI++ A E +P+ + DS K E+ V + K+P +SLGM+VAGG++S+ D PVY+TN+ PN ++ I KGDILL VN +L G++ AV LK + + V ++VL++ PN + L D++ P W+ WL+LP + + I L R + +GSLGFSIVGG + + N N S I+SIV G+PA+ +GR++CGDIL+ +N S ++H+ V L K +G + L +VSWPG+++
Sbjct: 281 LPQLHHLIPDGEVTSIKITRADPCEPLAISIVGGNETPLVRILIQDIYREGVIARDGRLLPGDMILKVNGIDISNVPH--CYAVAA-LKQPCTLLRLTVLREQRHRYRSHHHSPTEAFPAHTATIRDDSLHVVLVKRAPDEQLGIKLVRRPDEHGVFIFHLLEGGLAARDGRLRVDDRVLAINGHDLRYGAPEHAALLIQASEDRVHFIVSRQTHIPAPDILQEAPWNMEGPPPYSPVDIEHTL-------------------LDS--CQKPACYEKTVTLLKEPHDSLGMTVAGGMSSRGWDLPVYVTNVDPNGVVGQEGSIRKGDILLNVNGVDLTGVTRSEAVANLK---NTSSPVVLQVLEMRPPNESSLDCMPPLHSPCALSPSSPGDVKLPPPNDDYAPLWVSWLQLPRHLYCCKDIVLRR-STSGSLGFSIVGGQEEL---NCNQSF--------------FIRSIVEGTPAYNDGRIRCGDILLEVNGKSTWGMTHTALVRLLKELRGRITLTIVSWPGSLL 765
BLAST of Ligand of Numb protein X 2 vs. Ensembl Xenopus
Match: ddx54 (DEAD-box helicase 54 [Source:Xenbase;Acc:XB-GENE-1011576]) HSP 1 Score: 247.669 bits (631), Expect = 1.284e-71 Identity = 170/500 (34.00%), Postives = 263/500 (52.60%), Query Frame = 3
Query: 585 ITIINPER----LEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVA---------KEEILTFVLEKP-TDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKV-------PNLLLMANTD------NFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
IT I R +E G+S VGG +TPL +VIQEI+RDG IA DGRL GDQI ++N + +S SH R VLS Q ++LT+ R+ +R+ A ++E L K ++ ++G+KL +T++SG++I ++L A+ DGRL +DR+L IN DL GT A +II+ + V +SR + Q +I R G + T +V + + ++ +E+ + V K+P ESLGM+VAGG S+ G+ P+++T++ P+ L + I +GD+LL++N +L LSH AV LK A + V+++ ++V P M+ ++ PSW+ WL LP + L R ++ GS GFSIVGG Y N + +P IK+IV G+PA+ +GRLKCGD++I++N S +SHS V + K + V L V+SWPG+++
Sbjct: 229 ITTIEIHRSNPYIELGISIVGGNETPLINVVIQEIYRDGVIARDGRLLAGDQILQVNNFDISNVSHNYARWVLS---QPCTVLHLTVLRE---RRFGSRAHGHGDGGSQRDESFHITLNKRGSNEQLGIKLVRRTDESGVFILDLLEGGLAAQDGRLHSNDRVLAINGLDLKHGTPELAAQIIQGSGERVNLTVSRPGKHQPGNITRDLGMNQPHHHT------------------QQVYHHRPSAHKDLAQCVKCQEKHITVKKEPQESLGMTVAGGRGSKSGELPIFVTSVQPHGCLARDSRIKRGDVLLSINGIDLTNLSHSEAVAMLK-ASATSSVVSLKAIEVEVIEEQTPGKEEMSTVSENEYDASWSPSWVMWLGLPSCLHSCHDVVLRR-SSLGSWGFSIVGG----YEENHTN-------------QPFFIKTIVLGTPAYYDGRLKCGDMIIAVNGLSTVGMSHSALVPMLKEQRNKVTLSVISWPGSLV 685
BLAST of Ligand of Numb protein X 2 vs. Ensembl Xenopus
Match: LNX1 (ligand of numb-protein X 1 [Source:NCBI gene;Acc:100487348]) HSP 1 Score: 236.113 bits (601), Expect = 1.105e-67 Identity = 174/505 (34.46%), Postives = 258/505 (51.09%), Query Frame = 3
Query: 570 VEDFTITIINPERLEF----GVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDK-RWLPVAKEE------ILTFVLEKPT-DRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLK--------VPNLLLMANTDN-------FIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
+ D IT I R + G+ VGG +TPL I+IQ I++DG I DGRL PGD I K+N +S H LS + Q + LT+ R+ K R V E+ + VL K + D ++G+KL + ++ G+YI +L A+ DG L+++DR+L IN HDL GT A ++I++ ++ V F++SR R QTPDI++ A ++N +P ++ R Y F+ S E+VV + K ESLGM+VAGG ++ D P+++T++ + ++ + GDILL VN L G+S AV LK + + + +K + N L M N + P W+ WL LP Y R + L R+ A GSLGFSIVGG+D D S K P IKSI+ G+PAH +GRL+CGDIL+ +N S + ++ V + K +G V L +VSWPG+ +
Sbjct: 176 IPDGEITCIKINRADLNEILGIRIVGGNETPLTQIIIQHIYQDGVIGRDGRLLPGDMILKVNGIDISSVPHTYA---LSILRQPSKVLRLTVLREQRYKCRTNGVTMEQHSPQDDSIHVVLNKSSHDVQLGIKLVRRRDEPGIYIFNLLEGGVAARDGYLQENDRVLSINGHDLRNGTPEIAAQLIQASEEGVYFIVSRHSRHQTPDILQEARWH-NNNDSPATCTNE---------RNY--------FNKNASRTVLCHEKVVSITKDNTESLGMTVAGGASNWEWDLPIFVTSVDADGVIGRDGRLKTGDILLNVNGINLTGVSRSEAVSLLKNSSSSSVVLKALEIKEYEPEEDNLNNALYMDYLQNGSEQNREWSPVWVMWLNLPHYLYSCREVVLRRNTA-GSLGFSIVGGSD------------DCSRIK-----PFFIKSILEGTPAHHDGRLRCGDILLEVNGRCTSGMPYNSLVRMLKEIRGRVVLTIVSWPGSYL 641
BLAST of Ligand of Numb protein X 2 vs. Ensembl Xenopus
Match: LNX1 (ligand of numb-protein X 1 [Source:NCBI gene;Acc:100487348]) HSP 1 Score: 237.269 bits (604), Expect = 2.930e-67 Identity = 171/507 (33.73%), Postives = 260/507 (51.28%), Query Frame = 3
Query: 570 VEDFTITIINPERLEF----GVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRD---------IVDKRWLPVAKEEILTFVLEKPT-DRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLK--------VPNLLLMANTDN-------FIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
+ D IT I R + G+ VGG +TPL I+IQ I++DG I DGRL PGD I K+N +S H LS + Q + LT+ R+ + ++ P +++ + VL K + D ++G+KL + ++ G+YI +L A+ DG L+++DR+L IN HDL GT A ++I++ ++ V F++SR R QTPDI++ A ++N +P ++ R Y F+ S E+VV + K ESLGM+VAGG ++ D P+++T++ + ++ + GDILL VN L G+S AV LK + + + +K + N L M N + P W+ WL LP Y R + L R+ A GSLGFSIVGG+D D S K P IKSI+ G+PAH +GRL+CGDIL+ +N S + ++ V + K +G V L +VSWPG+ +
Sbjct: 277 IPDGEITCIKINRADLNEILGIRIVGGNETPLTQIIIQHIYQDGVIGRDGRLLPGDMILKVNGIDISSVPHTY---ALSILRQPSKVLRLTVLREQRYKCRTNGVTMEQHSP--QDDSIHVVLNKSSHDVQLGIKLVRRRDEPGIYIFNLLEGGVAARDGYLQENDRVLSINGHDLRNGTPEIAAQLIQASEEGVYFIVSRHSRHQTPDILQEARWH-NNNDSPATCTNE---------RNY--------FNKNASRTVLCHEKVVSITKDNTESLGMTVAGGASNWEWDLPIFVTSVDADGVIGRDGRLKTGDILLNVNGINLTGVSRSEAVSLLKNSSSSSVVLKALEIKEYEPEEDNLNNALYMDYLQNGSEQNREWSPVWVMWLNLPHYLYSCREVVLRRNTA-GSLGFSIVGGSD------------DCSRIK-----PFFIKSILEGTPAHHDGRLRCGDILLEVNGRCTSGMPYNSLVRMLKEIRGRVVLTIVSWPGSYL 742
BLAST of Ligand of Numb protein X 2 vs. Ensembl Xenopus
Match: c1qb (complement C1q B chain [Source:Xenbase;Acc:XB-GENE-6251287]) HSP 1 Score: 200.675 bits (509), Expect = 1.155e-54 Identity = 160/505 (31.68%), Postives = 243/505 (48.12%), Query Frame = 3
Query: 585 ITIINPERLEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVAKE------------EILTFVLEKPTDRK--MGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGL---KNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFI---------------PSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
I ++PE G+ VGG DTPL IV+QEI D +A DG++ PGD I ++N +S +H + ++ + Q + LT+ + +K + P + +++ L K DR +G+KL KT + G+++ ++L A DGRLK +D++L IN H+L GT A +II+S + V FV+ R + Q DI + + SN P L + ST +V P E+ V V K+ ESLG+++ GG + P+Y+++I P L I KGD+LL++N T+L LS+ A+ ALK N TV VLK + + D + P W+YWL LP R ITL R ++ S GFSIVGG F +P IK+IV G+PA + RLKCGD ++ +N S +S++ + + K + V L VVSWPG+++
Sbjct: 219 IHRVDPEN-PLGMRIVGGKDTPLGNIVVQEILTDSLVATDGKVTPGDHILEVNGTNISNVTHSQ---AIALLRQPCSVLQLTL---VQEKGFSPQSSRTENSTGGSPHSTKVIQVTLTK-RDRSEPLGIKLVRKTEEPGIFVLDLLDGGLAYKDGRLKCNDKVLSINGHNLKHGTPETAAQIIQSSEARVNFVVLRHMIDQPVDINEESSSSGSSNGGSPVHPRKRPSLRPNRRKSTHHKDVNPGP-----------PDHEKSVNVKKEQYESLGITIGGG-RDLKNKLPIYVSSIQPVGCLFRDGRIRKGDVLLSINGTDLTSLSYSDAISALK---TNAASHTV-VLKALEVTVGERLDEPVEHAAGVTREPGGIWSPLWVYWLALPSSLHWCRHITL-RKTSSESWGFSIVGG------FEEGKG-----------DQPFFIKTIVPGTPAFYDKRLKCGDEIVEVNGVSAVGMSNAQLIPMLKEQKNRVTLTVVSWPGSLV 681
BLAST of Ligand of Numb protein X 2 vs. Ensembl Xenopus
Match: nphp3 (nephronophthisis 3 (adolescent) [Source:Xenbase;Acc:XB-GENE-487873]) HSP 1 Score: 185.652 bits (470), Expect = 6.221e-50 Identity = 144/485 (29.69%), Postives = 234/485 (48.25%), Query Frame = 3
Query: 573 EDFTITIINPERLEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVAKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKN---ASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLP-----------GYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVS 1985
++ TI I + R G+S VGG DTPL+ IVI E++ +G A DGRL GDQI ++N L ASH D ++ + Q+ + LT++RD + ++ +I L+K R +GL + GK SG++I +++ A +DGRL + D+I+ +N D+ +Q ++K + V I R+ + + + T S + +++ P +S + Y S + VE+++ P ++LG+S+AGG S GD PV+I I + + T+ + GD L+++N+ + GLSH V LK A + ++V+ N+ + I S + + L G +P++I L + LGFSIVGG S DL PI +K+I S A +GRLK GD ++S+N SL V+H +AV + K +G V L V+S
Sbjct: 141 QEMTIEI-SKGRSGLGLSIVGGNDTPLEAIVIHEVYEEGAAARDGRLWAGDQILEVNGVDLRNASH---EDAITALRQTPQKVQLTVYRD--EAQYKDEENLDIFHVELQKKAGRGLGLSIVGKRTGSGVFISDIVKGGAADIDGRLMQGDQIMSVNGDDMRNASQEIVATVLKCAQGLVHLEIGRL---RVGSWLASKKTFEASQINQMSSHGVLPPSLTPVISSMQDYASSKKSSADSSQRHSGTDLEPRTVEINRGPYDALGISIAGGKGSPLGDIPVFIAMIQASGVAARTHKLKVGDRLVSINQQPVDGLSHAEVVNILKHA---FGTIVLQVVADTNI-------SAIASQLESMSLGQGVSSEHQVEDGESPVPKIIHLEKGGD--GLGFSIVGG--------YGSPQGDL---------PIYVKTIFSKGAAAADGRLKRGDQILSVNGESLEGVTHDEAVAILKKQRGNVTLSVLS 587 HSP 2 Score: 71.2478 bits (173), Expect = 3.362e-12 Identity = 65/230 (28.26%), Postives = 97/230 (42.17%), Query Frame = 3
Query: 1317 EVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNC------NKVTVRVLKV-PNLLLMANTDNFIPSWMYWLKLPGYCQI--PRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKV 1979
++E++K LG+S+AG N R +++ I P+ I GD LL +N + G SH+ A +K A NK V+ + V P+ L N + +PS+ P C I + +T+ LG SIVGGND P I+I + A ++GRL GD ++ +N L N SH DA+ + T V L V
Sbjct: 21 HIIELEKDK-NGLGLSLAG--NKDRSRMSIFVVAINPDGPAGQDGRIHVGDELLEINNQIIYGKSHQNASAIIKGAPSTLKLVFIRNKDAVQQMAVNPDPLPERNMPSPLPSFD-----PATCPIIPGQEMTIEISKGRSGLGLSIVGGND-------------------TPLEAIVIHEVYEEGAAARDGRLWAGDQILEVNGVDLRNASHEDAITALRQTPQKVQLTV 223
BLAST of Ligand of Numb protein X 2 vs. Ensembl Mouse
Match: Lnx2 (ligand of numb-protein X 2 [Source:MGI Symbol;Acc:MGI:2155959]) HSP 1 Score: 262.692 bits (670), Expect = 3.756e-77 Identity = 179/508 (35.24%), Postives = 264/508 (51.97%), Query Frame = 3
Query: 564 FNVEDFTITIINPER----LEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVA---------KEEILTFVLEK-PTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKV-------------PNLLLMANTD-NFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
++ + IT I R ++ G+S VGG +TPL IVIQE++RDG IA DGRL GDQI ++N Y +S SH R VLS Q T+ LT+ R+ +R+ A ++E+ +L K + ++G+KL +T++ G++I ++L A+ DGRL +DR+L IN HDL GT A +II++ + V I+R +PQ + R AG SN S S PG TR T +E+ + V K+P ESLGM+VAGG S+ G+ P+++T++ P+ L I +GD+LL +N +L LSH AV LK A V ++ L+V P+ D ++ PSW+ WL LP I L R + GS GFSIVGG Y N + +P IK+IV G+PA+ +GRLKCGD+++++N S +SHS V + K + V L V+ WPG+++
Sbjct: 223 LSLPEGEITTIEIHRSNPYIQLGISIVGGNETPLINIVIQEVYRDGVIARDGRLLAGDQILQVNNYDISNVSHNHARAVLS---QPCSTLQLTVLRE---RRFGSRANSHADGSAPRDEVFQVLLHKRDSTEQLGIKLVRRTDEPGVFILDLLEGGLAAQDGRLNSNDRVLAINGHDLKHGTPELAAQIIQASGERVNLTIARPGKPQPSNGSREAGAHSSSNHAQ-PPSHSRPGSHKDLTRCV-----------------TCQEKHITVKKEPHESLGMTVAGGRGSKSGELPIFVTSVPPHGCLARDGRIKRGDVLLNINGIDLTNLSHSEAVAMLK-ASAASPAVILKALEVQIAEEAAQATEEQPSAFSENEYDASWSPSWVMWLGLPSALHSCHDIVLRR-SYLGSWGFSIVGG----YEENHTN-------------QPFFIKTIVLGTPAYYDGRLKCGDMIVAVNGLSTVGMSHSALVPMLKEQRNKVTLTVICWPGSLV 687
BLAST of Ligand of Numb protein X 2 vs. Ensembl Mouse
Match: Lnx1 (ligand of numb-protein X 1 [Source:MGI Symbol;Acc:MGI:1278335]) HSP 1 Score: 243.817 bits (621), Expect = 8.841e-71 Identity = 174/521 (33.40%), Postives = 264/521 (50.67%), Query Frame = 3
Query: 543 NHLDNLEFNVEDFT------ITIINPERLE----FGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDI---------VDKRWLPVAKEEILTFVLEKPT-DRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPN---------LLLMANTD-----NFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
NH+D N E+ T IT I R + + VGG +TPL I+IQ I+RDG IA DGRL PGD I K+N +S H +L + Q + LT+ R+ V + P +++ +L K + + ++G+KL + ++ G++I VL A G+L+++DR+L IN HDL FG+ A +I++ + V V+SR VR +PDI + AG + +P PG +N +++ +T E+VV V K P ESLGM+V GG + + D P+Y+ ++ P ++ I GDILL VN EL +S AV LK A + V ++ L+V L +N + ++ PSW+ WL+LP Y + + L R+ A GSLGF IVGG + + S NK P IKSIV G+PA+ +GR++CGDIL+++N S S + H+ + K +G + L + SWPGT +
Sbjct: 146 NHVDQGRDNSENTTVPEDGEITSIKINRADPSESLSIRLVGGSETPLVHIIIQHIYRDGVIARDGRLLPGDIILKVNGMDISNVPHNYAVRLLRQPCQ---VLRLTVLREQKFRSRSNAHVPDSYGP--RDDSFHVILNKSSPEEQLGIKLVRRVDEPGVFIFNVLNGGVADRHGQLEENDRVLAINGHDLRFGSPESAAHLIQASERRVHLVVSRQVRQSSPDIFQEAGWISNGQQSP------GPGERNTASKP----------------AATCHEKVVSVWKDPSESLGMTVGGGASHREWDLPIYVISVEPGGVISRDGRIKTGDILLNVNGIELTEVSRTEAVAILKSA---PSSVVLKALEVKEQEAQEDCSPAALDSNHNVTPPGDWSPSWVMWLELPQYLCNCKDVILRRNTA-GSLGFCIVGGYE------------EYSGNK-----PFFIKSIVEGTPAYNDGRIRCGDILLAVNGRSTSGMIHACLARMLKELKGRITLTIASWPGTFL 618
BLAST of Ligand of Numb protein X 2 vs. Ensembl Mouse
Match: Lnx1 (ligand of numb-protein X 1 [Source:MGI Symbol;Acc:MGI:1278335]) HSP 1 Score: 240.35 bits (612), Expect = 4.381e-70 Identity = 169/506 (33.40%), Postives = 258/506 (50.99%), Query Frame = 3
Query: 570 VEDFTITIINPERLE----FGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDI---------VDKRWLPVAKEEILTFVLEKPT-DRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPN---------LLLMANTD-----NFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
+ D IT I R + + VGG +TPL I+IQ I+RDG IA DGRL PGD I K+N +S H +L + Q + LT+ R+ V + P +++ +L K + + ++G+KL + ++ G++I VL A G+L+++DR+L IN HDL FG+ A +I++ + V V+SR VR +PDI + AG + +P PG +N +++ +T E+VV V K P ESLGM+V GG + + D P+Y+ ++ P ++ I GDILL VN EL +S AV LK A + V ++ L+V L +N + ++ PSW+ WL+LP Y + + L R+ A GSLGF IVGG + + S NK P IKSIV G+PA+ +GR++CGDIL+++N S S + H+ + K +G + L + SWPGT +
Sbjct: 112 IPDGEITSIKINRADPSESLSIRLVGGSETPLVHIIIQHIYRDGVIARDGRLLPGDIILKVNGMDISNVPHNYAVRLLRQPCQ---VLRLTVLREQKFRSRSNAHVPDSYGP--RDDSFHVILNKSSPEEQLGIKLVRRVDEPGVFIFNVLNGGVADRHGQLEENDRVLAINGHDLRFGSPESAAHLIQASERRVHLVVSRQVRQSSPDIFQEAGWISNGQQSP------GPGERNTASKP----------------AATCHEKVVSVWKDPSESLGMTVGGGASHREWDLPIYVISVEPGGVISRDGRIKTGDILLNVNGIELTEVSRTEAVAILKSA---PSSVVLKALEVKEQEAQEDCSPAALDSNHNVTPPGDWSPSWVMWLELPQYLCNCKDVILRRNTA-GSLGFCIVGGYE------------EYSGNK-----PFFIKSIVEGTPAYNDGRIRCGDILLAVNGRSTSGMIHACLARMLKELKGRITLTIASWPGTFL 569
BLAST of Ligand of Numb protein X 2 vs. Ensembl Mouse
Match: Lnx1 (ligand of numb-protein X 1 [Source:MGI Symbol;Acc:MGI:1278335]) HSP 1 Score: 240.35 bits (612), Expect = 4.381e-70 Identity = 169/506 (33.40%), Postives = 258/506 (50.99%), Query Frame = 3
Query: 570 VEDFTITIINPERLE----FGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDI---------VDKRWLPVAKEEILTFVLEKPT-DRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPN---------LLLMANTD-----NFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
+ D IT I R + + VGG +TPL I+IQ I+RDG IA DGRL PGD I K+N +S H +L + Q + LT+ R+ V + P +++ +L K + + ++G+KL + ++ G++I VL A G+L+++DR+L IN HDL FG+ A +I++ + V V+SR VR +PDI + AG + +P PG +N +++ +T E+VV V K P ESLGM+V GG + + D P+Y+ ++ P ++ I GDILL VN EL +S AV LK A + V ++ L+V L +N + ++ PSW+ WL+LP Y + + L R+ A GSLGF IVGG + + S NK P IKSIV G+PA+ +GR++CGDIL+++N S S + H+ + K +G + L + SWPGT +
Sbjct: 112 IPDGEITSIKINRADPSESLSIRLVGGSETPLVHIIIQHIYRDGVIARDGRLLPGDIILKVNGMDISNVPHNYAVRLLRQPCQ---VLRLTVLREQKFRSRSNAHVPDSYGP--RDDSFHVILNKSSPEEQLGIKLVRRVDEPGVFIFNVLNGGVADRHGQLEENDRVLAINGHDLRFGSPESAAHLIQASERRVHLVVSRQVRQSSPDIFQEAGWISNGQQSP------GPGERNTASKP----------------AATCHEKVVSVWKDPSESLGMTVGGGASHREWDLPIYVISVEPGGVISRDGRIKTGDILLNVNGIELTEVSRTEAVAILKSA---PSSVVLKALEVKEQEAQEDCSPAALDSNHNVTPPGDWSPSWVMWLELPQYLCNCKDVILRRNTA-GSLGFCIVGGYE------------EYSGNK-----PFFIKSIVEGTPAYNDGRIRCGDILLAVNGRSTSGMIHACLARMLKELKGRITLTIASWPGTFL 569
BLAST of Ligand of Numb protein X 2 vs. Ensembl Mouse
Match: Lnx1 (ligand of numb-protein X 1 [Source:MGI Symbol;Acc:MGI:1278335]) HSP 1 Score: 241.506 bits (615), Expect = 7.143e-70 Identity = 169/506 (33.40%), Postives = 258/506 (50.99%), Query Frame = 3
Query: 570 VEDFTITIINPERLE----FGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDI---------VDKRWLPVAKEEILTFVLEKPT-DRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPN---------LLLMANTD-----NFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
+ D IT I R + + VGG +TPL I+IQ I+RDG IA DGRL PGD I K+N +S H +L + Q + LT+ R+ V + P +++ +L K + + ++G+KL + ++ G++I VL A G+L+++DR+L IN HDL FG+ A +I++ + V V+SR VR +PDI + AG + +P PG +N +++ +T E+VV V K P ESLGM+V GG + + D P+Y+ ++ P ++ I GDILL VN EL +S AV LK A + V ++ L+V L +N + ++ PSW+ WL+LP Y + + L R+ A GSLGF IVGG + + S NK P IKSIV G+PA+ +GR++CGDIL+++N S S + H+ + K +G + L + SWPGT +
Sbjct: 171 IPDGEITSIKINRADPSESLSIRLVGGSETPLVHIIIQHIYRDGVIARDGRLLPGDIILKVNGMDISNVPHNYAVRLLRQPCQ---VLRLTVLREQKFRSRSNAHVPDSYGP--RDDSFHVILNKSSPEEQLGIKLVRRVDEPGVFIFNVLNGGVADRHGQLEENDRVLAINGHDLRFGSPESAAHLIQASERRVHLVVSRQVRQSSPDIFQEAGWISNGQQSP------GPGERNTASKP----------------AATCHEKVVSVWKDPSESLGMTVGGGASHREWDLPIYVISVEPGGVISRDGRIKTGDILLNVNGIELTEVSRTEAVAILKSA---PSSVVLKALEVKEQEAQEDCSPAALDSNHNVTPPGDWSPSWVMWLELPQYLCNCKDVILRRNTA-GSLGFCIVGGYE------------EYSGNK-----PFFIKSIVEGTPAYNDGRIRCGDILLAVNGRSTSGMIHACLARMLKELKGRITLTIASWPGTFL 628
BLAST of Ligand of Numb protein X 2 vs. UniProt/SwissProt
Match: sp|Q8N448|LNX2_HUMAN (Ligand of Numb protein X 2 OS=Homo sapiens OX=9606 GN=LNX2 PE=1 SV=1) HSP 1 Score: 266.159 bits (679), Expect = 1.130e-77 Identity = 181/508 (35.63%), Postives = 266/508 (52.36%), Query Frame = 3
Query: 585 ITIINPER----LEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVA---------KEEILTFVLEK-PTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDS--NMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKS-----TYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKV-------------PNLLLMANTD-NFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
IT I R ++ G+S VGG +TPL IVIQE++RDG IA DGRL GDQI ++N Y +S SH R VLS Q +T++LT+ R+ +R+ A +EEI L K + ++G+KL +T++ G++I ++L A+ DGRL +DR+L IN HDL +GT A +II++ + V I+R +PQ + IR AG S + TP P + P S K T +E+ + V K+P ESLGM+VAGG S+ G+ P+++T++ P+ L I +GD+LL +N +L LSH AV LK A V ++ L+V P+ D ++ PSW+ WL LP I L R + GS GFSIVGG + + TN +P IK+IV G+PA+ +GRLKCGD+++++N S +SHS V + K + V L V+ WPG+++
Sbjct: 231 ITTIEIHRSNPYIQLGISIVGGNETPLINIVIQEVYRDGVIARDGRLLAGDQILQVNNYNISNVSHNYARAVLS---QPCNTLHLTVLRE---RRFGNRAHNHSDSNSPREEIFQVALHKRDSGEQLGIKLVRRTDEPGVFILDLLEGGLAAQDGRLSSNDRVLAINGHDLKYGTPELAAQIIQASGERVNLTIARPGKPQPGNTIREAGNHSSSSQHHTP-----------------------PPYYSRPSSHKDLTQCVTCQEKHITVKKEPHESLGMTVAGGRGSKSGELPIFVTSVPPHGCLARDGRIKRGDVLLNINGIDLTNLSHSEAVAMLK-ASAASPAVALKALEVQIVEEATQNAEEQPSTFSENEYDASWSPSWVMWLGLPSTLHSCHDIVLRR-SYLGSWGFSIVGGYEENH---TN--------------QPFFIKTIVLGTPAYYDGRLKCGDMIVAVNGLSTVGMSHSALVPMLKEQRNKVTLTVICWPGSLV 690
BLAST of Ligand of Numb protein X 2 vs. UniProt/SwissProt
Match: sp|Q91XL2|LNX2_MOUSE (Ligand of Numb protein X 2 OS=Mus musculus OX=10090 GN=Lnx2 PE=1 SV=2) HSP 1 Score: 262.692 bits (670), Expect = 2.627e-76 Identity = 179/508 (35.24%), Postives = 264/508 (51.97%), Query Frame = 3
Query: 564 FNVEDFTITIINPER----LEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVA---------KEEILTFVLEK-PTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKV-------------PNLLLMANTD-NFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
++ + IT I R ++ G+S VGG +TPL IVIQE++RDG IA DGRL GDQI ++N Y +S SH R VLS Q T+ LT+ R+ +R+ A ++E+ +L K + ++G+KL +T++ G++I ++L A+ DGRL +DR+L IN HDL GT A +II++ + V I+R +PQ + R AG SN S S PG TR T +E+ + V K+P ESLGM+VAGG S+ G+ P+++T++ P+ L I +GD+LL +N +L LSH AV LK A V ++ L+V P+ D ++ PSW+ WL LP I L R + GS GFSIVGG Y N + +P IK+IV G+PA+ +GRLKCGD+++++N S +SHS V + K + V L V+ WPG+++
Sbjct: 223 LSLPEGEITTIEIHRSNPYIQLGISIVGGNETPLINIVIQEVYRDGVIARDGRLLAGDQILQVNNYDISNVSHNHARAVLS---QPCSTLQLTVLRE---RRFGSRANSHADGSAPRDEVFQVLLHKRDSTEQLGIKLVRRTDEPGVFILDLLEGGLAAQDGRLNSNDRVLAINGHDLKHGTPELAAQIIQASGERVNLTIARPGKPQPSNGSREAGAHSSSNHAQ-PPSHSRPGSHKDLTRCV-----------------TCQEKHITVKKEPHESLGMTVAGGRGSKSGELPIFVTSVPPHGCLARDGRIKRGDVLLNINGIDLTNLSHSEAVAMLK-ASAASPAVILKALEVQIAEEAAQATEEQPSAFSENEYDASWSPSWVMWLGLPSALHSCHDIVLRR-SYLGSWGFSIVGG----YEENHTN-------------QPFFIKTIVLGTPAYYDGRLKCGDMIVAVNGLSTVGMSHSALVPMLKEQRNKVTLTVICWPGSLV 687
BLAST of Ligand of Numb protein X 2 vs. UniProt/SwissProt
Match: sp|Q8TBB1|LNX1_HUMAN (E3 ubiquitin-protein ligase LNX OS=Homo sapiens OX=9606 GN=LNX1 PE=1 SV=1) HSP 1 Score: 253.447 bits (646), Expect = 1.421e-72 Identity = 180/541 (33.27%), Postives = 274/541 (50.65%), Query Frame = 3
Query: 462 ISNTNSGNNFPKPQDCFYQNNSFYTPLNHLDNLEFNVEDFTITIINPERLE----FGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRD----IVDKRWLPVA---KEEILTFVLEKPT-DRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKV-----------PNLL----LMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
+ T SG+ D +N+ T L + D IT I R++ + VGG +TPL I+IQ I+RDG IA DGRL PGD I K+N +S H +L + Q ++LT+ R+ + P A +++ +L K + + ++G+KL K ++ G++I VL A G+L+++DR+L IN HDL +G+ A +I++ + V V+SR VR ++PDI + AG + + +P PG E N+P P+ T E+VV + K PGESLGM+VAGG + + D P+Y+ ++ P ++ I GDILL V+ EL +S AV LK + + ++ L+V P L MA ++ PSW+ WL+LP + I L R+ A GSLGF IVGG + +N N +P IKSIV G+PA+ +GR++CGDIL+++N S S + H+ L K +G + L +VSWPGT +
Sbjct: 231 LRRTKSGSAVANHADQGRENSENTTAPEVFPRLYHLIPDGEITSIKINRVDPSESLSIRLVGGSETPLVHIIIQHIYRDGVIARDGRLLPGDIILKVNGMDISNVPHNYAVRLLRQPCQ---VLWLTVMREQKFRSRNNGQAPDAYRPRDDSFHVILNKSSPEEQLGIKLVRKVDEPGVFIFNVLDGGVAYRHGQLEENDRVLAINGHDLRYGSPESAAHLIQASERRVHLVVSRQVRQRSPDIFQEAGWNSNGSWSP------GPG---------ERSNTP----KPLHPTITCHEKVVNIQKDPGESLGMTVAGGASHREWDLPIYVISVEPGGVISRDGRIKTGDILLNVDGVELTEVSRSEAVALLKRTS---SSIVLKALEVKEYEPQEDCSSPAALDSNHNMAPPSDWSPSWVMWLELPRCLYNCKDIVLRRNTA-GSLGFCIVGGYEE---YNGN--------------KPFFIKSIVEGTPAYNDGRIRCGDILLAVNGRSTSGMIHACLARLLKELKGRITLTIVSWPGTFL 728
BLAST of Ligand of Numb protein X 2 vs. UniProt/SwissProt
Match: sp|O70263|LNX1_MOUSE (E3 ubiquitin-protein ligase LNX OS=Mus musculus OX=10090 GN=Lnx1 PE=1 SV=1) HSP 1 Score: 241.891 bits (616), Expect = 2.394e-68 Identity = 169/506 (33.40%), Postives = 258/506 (50.99%), Query Frame = 3
Query: 570 VEDFTITIINPERLE----FGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDI---------VDKRWLPVAKEEILTFVLEKPT-DRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPN---------LLLMANTD-----NFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
+ D IT I R + + VGG +TPL I+IQ I+RDG IA DGRL PGD I K+N +S H +L + Q + LT+ R+ V + P +++ +L K + + ++G+KL + ++ G++I VL A G+L+++DR+L IN HDL FG+ A +I++ + V V+SR VR +PDI + AG + +P PG +N +++ +T E+VV V K P ESLGM+V GG + + D P+Y+ ++ P ++ I GDILL VN EL +S AV LK A + V ++ L+V L +N + ++ PSW+ WL+LP Y + + L R+ A GSLGF IVGG + + S NK P IKSIV G+PA+ +GR++CGDIL+++N S S + H+ + K +G + L + SWPGT +
Sbjct: 271 IPDGEITSIKINRADPSESLSIRLVGGSETPLVHIIIQHIYRDGVIARDGRLLPGDIILKVNGMDISNVPHNYAVRLLRQPCQ---VLRLTVLREQKFRSRSNAHVPDSYGP--RDDSFHVILNKSSPEEQLGIKLVRRVDEPGVFIFNVLNGGVADRHGQLEENDRVLAINGHDLRFGSPESAAHLIQASERRVHLVVSRQVRQSSPDIFQEAGWISNGQQSP------GPGERNTASKP----------------AATCHEKVVSVWKDPSESLGMTVGGGASHREWDLPIYVISVEPGGVISRDGRIKTGDILLNVNGIELTEVSRTEAVAILKSAP---SSVVLKALEVKEQEAQEDCSPAALDSNHNVTPPGDWSPSWVMWLELPQYLCNCKDVILRRNTA-GSLGFCIVGGYE------------EYSGNK-----PFFIKSIVEGTPAYNDGRIRCGDILLAVNGRSTSGMIHACLARMLKELKGRITLTIASWPGTFL 728
BLAST of Ligand of Numb protein X 2 vs. UniProt/SwissProt
Match: sp|Q8VBX6|MPDZ_MOUSE (Multiple PDZ domain protein OS=Mus musculus OX=10090 GN=Mpdz PE=1 SV=2) HSP 1 Score: 179.874 bits (455), Expect = 1.731e-45 Identity = 137/467 (29.34%), Postives = 226/467 (48.39%), Query Frame = 3
Query: 615 FGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVAKEEI---LTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNL-LLMANTDNFIPSWMYWLKL------PGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVS 1985
G+S VGG DT L I+I E++ +G DGRL GDQI ++N L +A+H E +VL + Q + LT++RD P +E++ T L+K + +GL + GK N +G+++ +++ A DGRL + D+IL +N D+ TQ ++K +V + R+ R + + S + + + G+ + + E + S I T VE+ K P +SLG+S+AGGV S GD P++I + PN + T + GD ++ + T G++H +AV +K A + V+V+ ++ ++ + + + L P P+ T++ D LGFSIVGG S DL PI +K++ + A ++GRLK GD +I++N SL V+H +AV + K T+G V L V+S
Sbjct: 1624 LGLSIVGGSDTLLGAIIIHEVYEEGAACKDGRLWAGDQILEVNGIDLRKATHDEAINVLRQTPQR---VRLTLYRD-----EAPYKEEDVCDTFTIELQKRPGKGLGLSIVGKRNDTGVFVSDIVKGGIADADGRLMQGDQILMVNGEDVRHATQEAVAALLKCSLGAVTLEVGRVKAAPFHSERRPSQSSQVSESSLSSFTPPLSGINTSES--LESNSKKNALASEIQGLRT-----VEIKKGPADSLGLSIAGGVGSPLGDVPIFIAMMHPNGVAAQTQKLRVGDRIVTICGTSTDGMTHTQAVNLMKNA---SGSIEVQVVAGGDVSVVTGHQQELANPCLAFTGLTSSSIFPDDLGPPQSKTITLDRGPDGLGFSIVGG--------YGSPHGDL---------PIYVKTVFAKGAAAEDGRLKRGDQIIAVNGQSLEGVTHEEAVAILKRTKGTVTLMVLS 2055 HSP 2 Score: 68.9366 bits (167), Expect = 1.457e-10 Identity = 85/378 (22.49%), Postives = 155/378 (41.01%), Query Frame = 3
Query: 891 FVLEKPTDRKMGLKLAGKTNKS----GLYIQEVLPDSQASLDGRLKKDDRILKINEHDL-VFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVR--VLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRM-----ITLSRDAATGSLGFSIVG--GNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVV 1982
F L KP +G + G +++ G+++QE+ S A DGRLK+ D+IL IN L T +A+ I++ KD+V+ VI+R ++ P+++ AST +P+ ++ E+V G LG + GG T V + I P + + GD +L + +T+L G+S E+ + L+ + + R V + P + T + S +++ Q + L+++ LG +I G G+ + P I +KSI S +GR++ GD +I+++ +L ++ AV + + T V L ++
Sbjct: 139 FELLKPPCGGLGFSVVGLRSENRGELGIFVQEIQEGSVAHRDGRLKETDQILAINGQVLDQTITHQQAISILQKAKDTVQLVIAR------------------GSLPPVSSPRISRSPSAASTIS--------AHSNPMHWQHVETIELV----NDGSGLGFGIIGGKA-----TGVIVKTILPGGVADQHGRLCSGDHILKIGDTDLAGMSSEQVAQVLRQCGNRVKLMIARGAVEETPASSSLGITLSSSTSSTSEMRVDASTQKNDESETFDVELTKNVQ--GLGITIAGYIGDKKLEPSG------------------IFVKSITKSSAVEHDGRIQIGDQIIAVDGTNLQGFTNQQAVEVLRHTGQTVRLTLM 461 HSP 3 Score: 60.4622 bits (145), Expect = 6.012e-8 Identity = 53/223 (23.77%), Postives = 91/223 (40.81%), Query Frame = 3
Query: 1317 EVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETEL-LGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVV 1982
E+ E+ K P LG SV G + RG+ +++ I S+ + + D +LA+N L ++H++A+ L+ A+D V R P + S + P + Q I L D + LGF I+GG +++K+I+ G A ++GRL GD ++ I D L+ +S + + V L +
Sbjct: 137 EIFELLKPPCGGLGFSVVGLRSENRGELGIFVQEIQEGSVAHRDGRLKETDQILAINGQVLDQTITHQQAISILQKAKDTVQLVIARGSLPPVSSPRISRSPSAASTISAHSNPMHWQHVETIELVNDGS--GLGFGIIGGK----------------------ATGVIVKTILPGGVADQHGRLCSGDHILKIGDTDLAGMSSEQVAQVLRQCGNRVKLMIA 335 HSP 4 Score: 53.5286 bits (127), Expect = 7.756e-6 Identity = 40/105 (38.10%), Postives = 51/105 (48.57%), Query Frame = 3
Query: 1671 PRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVS 1985
PR + L R+ + SLG SIVGG S L+ N E R I IK ++ SPA KNG LK GD +I ++ L + SH AV + PV V S
Sbjct: 1137 PRRVELWREPSK-SLGISIVGGR------GMGSRLS----NGEV-MRGIFIKHVLEDSPAGKNGTLKPGDRIIEVDGMDLRDASHEQAVEAIRKAGNPVVFMVQS 1229
BLAST of Ligand of Numb protein X 2 vs. TrEMBL
Match: A0A504YN76 (Ligand of Numb protein X 2 OS=Fasciola gigantica OX=46835 GN=FGIG_03645 PE=4 SV=1) HSP 1 Score: 469.929 bits (1208), Expect = 1.858e-149 Identity = 277/661 (41.91%), Postives = 386/661 (58.40%), Query Frame = 3
Query: 171 DRLMSVTVTSSPCQEFIPKSEIDPNLIKQCQGLFVSCCYSTAGCTFKAVGREVAAHEWQCPYGQKSPEXXXXXXXXXXXXXVFQT--------DAAGVSINGSQDISNTNSGNNFPKPQDCFYQNNSFYTPLN----HLDNLEFNVED--FTITIINPERLEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPV----------AKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSS-----SYPGLKNAST-----------RGYE-VGNSPFTFDSPISFKS-TYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYP------FNTNSSLTDLSH--NKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
D+L+ + S C+E + + +++ +L C+G V+C + GC + + + AH W C + P++ + + V + D G + + N +S + Q +Q ++ P + + +F VE +I + R E G+S VGG DTPL C++IQEI+ DG A DGRLRPGDQI ++N L+ A+H++ +LS S +G LT++R+ + + AK+E+L VL K ++++G+KLAGK N GLY+ ++PD QA LDGRL+KDDRIL+IN DL GTQ +A ++I KD V VI R +RPQTPDIIRAA E S +TS + P A T RG + N P D ++ + +E V+ + K+PGESLGMSVAGGV SQRGD P+Y+TN+APN L+ + + +GDILLAVN+ ELLGLSHERAVEALK ARD C +VT+R+LK P L NFIPSW++WL+LP YCQIPR++ L RD G LGFSI+GGND +P NT + TD S K RPI+IKSIV GSP + +GRLKCGDIL+S++ + L+N+SH+ AV L K G V L++VSWPGTI+
Sbjct: 333 DKLLVICPNSEHCREILARCDLESHLAYWCRGAVVACVNNKLGCPYLGLRAKQPAHRWNCQFQPSVPQTITIADAPQISDRVAASTSPQNSPRDQRSSDSGGPRLLGNESSVKSAAPSQSGSHQESTSSAPCSTKETSTEAAQFAVEGQIGSIDLKLDGRSELGISLVGGCDTPLLCMIIQEIYLDGIAARDGRLRPGDQILEVNGEELAHATHLQACLMLS--SLTGSVCRLTVYREQAVEAGHSLSSPASQHRIDAKQELLRIVLPKSQEKRLGIKLAGKKNCLGLYVLGLVPDGQAQLDGRLRKDDRILEINGVDLSNGTQEQAAQLIADAKDQVTLVILRQLRPQTPDIIRAASGENVSKCVVFSTSGQQSTVASPAPVRAITCTTAGNPPPPPRGITTLSNVPLEPDVQLAAGARVCQERVLVLTKRPGESLGMSVAGGVASQRGDVPIYVTNLAPNGLVARSGRVFRGDILLAVNDIELLGLSHERAVEALKNARDTCTQVTLRLLKGPECSL--EDRNFIPSWLFWLQLPRYCQIPRLVVLVRDPVIG-LGFSIIGGND-FFPDANETVTNTTAKETDGSDGTRKTPTPRPIVIKSIVPGSPCYWDGRLKCGDILLSVDHYPLTNISHAHAVALLKHCTGEVKLRIVSWPGTIV 987
BLAST of Ligand of Numb protein X 2 vs. TrEMBL
Match: A0A0X3PH35 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=TR160888 PE=4 SV=1) HSP 1 Score: 458.373 bits (1178), Expect = 1.769e-148 Identity = 280/675 (41.48%), Postives = 385/675 (57.04%), Query Frame = 3
Query: 171 DRLMSVTVTSSPCQEFIPKSEIDPNLIKQCQGLFVSCCYSTAGCTFKAVGREVAAHEWQCPYGQKSPEX-------XXXXXXXXXXXXVFQTDAAGVSINGSQDISNTNSGNNFPKPQDCFYQNNSFYTPLNHLDNLEFN---------VEDFTITIIN-----PERLEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWL---------------PVAKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAG----TEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFD--SPISFKS----TYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGN-----DPIYPFNTNSSLTDLSHNK-------------EAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
D+L+ + + C E + + +++ +L C+G + C + GC F+A H +C + +S + S+ V QT S GS S G P P Q+ + +N + + + D +T I ERL G+SFVGG DTPL C+VIQEI+ DG +A+DGRLRPGDQI + N L+QA+H + R +LS I+ S LT++R+ P +EEIL L K D+++G+KL GK N GLYI V+PDS+A LDGRL KDDRIL+IN DL GTQ+EA II+S + V V++R++RPQTPDIIR A + ++ T + P L VG + + SP S KS RE+V+ ++K+PGESLGM+VAGGV SQRGDTPVY+T+ P S++ + KGD+LLAVNE ELLGLSHERAVEALK R C+KVT+R+L+ P NF+PSW+YWL+LP YCQIP+ I LSR +A+GS GFSIVGGN DP P T + LT+ S + + +PI++KSIV G+PAH++GRLKCGD+++++N FSL++VSH+ AV L K+ G V ++VVSWPGTI+
Sbjct: 37 DKLLVICPNTEHCDEVLCRCDLESHLTYWCRGSVIPCLNAKTGCQFRAARALHTEHRRECRFQTRSTTANFSGGGGGGIVGPGSAFDVVHQTSPVVRSSTGSGAKSAVRFG---PMPSRRAAQDQTGRADVNASSLVVGSKPRTRSVSPIRDCEVTTIELPWQPNERL--GISFVGGSDTPLLCVVIQEIYLDGLVALDGRLRPGDQILETNGVDLTQATHHQARVLLSSITAS-RPCTLTVYRERASSETGSLNSGKLSSTATGDNPFEREEILHINLLKRLDKRLGIKLVGKKNLPGLYILGVVPDSEADLDGRLHKDDRILEINGVDLREGTQDEAANIIRSAGEHVSLVVARVIRPQTPDIIRTASGDIVIDYGADCTATTAGAGPPALVPPV-----VGPATLSLGAISPPSLKSPSTRLCREQVLVINKRPGESLGMTVAGGVASQRGDTPVYVTDFDPGSVVGRMGRLSKGDVLLAVNEVELLGLSHERAVEALKSTRKTCSKVTLRLLEGPE--TSTAKGNFLPSWLYWLQLPRYCQIPKTIVLSRTSASGSFGFSIVGGNNSMSADPASP--TMTVLTNASQREVTRPSATSSSSCVSSSPQPIVVKSIVPGTPAHRDGRLKCGDLILAVNQFSLADVSHAYAVALLKNCVGDVQIRVVSWPGTIV 696
BLAST of Ligand of Numb protein X 2 vs. TrEMBL
Match: A0A4E0RU30 (Ligand of Numb protein X 2 OS=Fasciola hepatica OX=6192 GN=D915_003900 PE=4 SV=1) HSP 1 Score: 465.692 bits (1197), Expect = 8.849e-148 Identity = 278/664 (41.87%), Postives = 386/664 (58.13%), Query Frame = 3
Query: 171 DRLMSVTVTSSPCQEFIPKSEIDPNLIKQCQGLFVSCCYSTAGCTFKAVGREVAAHEWQCPYGQKSPEXXXXXXXXXXXXXVFQTDAAGVSINGSQDISNTNSG------------NNFPKPQDCFYQNNS---FYTPLNHLDNLEFNVED--FTITIINPERLEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPV----------AKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSS-----SYPGLKNAST-----------RGYE-VGNSPFTFDSPISFKS-TYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYP------FNTNSSLTDL--SHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
D+L+ + S C+E + + +++ +L C+G V+C + GC + + + AH W C + P++ + + V A+ S N +D +++SG + P +++ S T + +F VE +I + R E G+S VGG DTPL C++IQEI+ DG A DGRLRPGDQI ++N L+ +H++ +LS S +G LT++R+ + + AK+E+L VL K ++++G+KLAGK N GLY+ ++PD QA LDGRL+KDDRIL+IN DL GTQ +A ++I KD V VI R VRPQTPDIIRAA E S +TS + P A T RG + N P D ++ + +E V+ + K+PGESLGMSVAGGV SQRGD P+Y+TN+APN L+ + + +GDILLAVN+ ELLGLSHERAVEALK ARD C +VT+R+LK P L NFIPSW++WL+LP YCQIPR++ L RD G LGFSI+GGND +P NT + TD K RPI+IKSIV GSP + +GRLKCGDIL+S++ + L+N+SH+ AV L K G V L++VSWPGTI+
Sbjct: 333 DKLLVICPNSEHCREILARCDLESHLAYWCRGAVVACVNNKLGCPYLGLRAKQPAHRWNCQFQPSVPQTITIADAPQISDRVA---ASTSSRNSPRDQRSSDSGGLRLLGNESSVKSAAPSQSGSHHESTSSAPCSTQETSAEAAQFAVEGQIGSIDLKLDGRSELGISLVGGCDTPLLCMIIQEIYLDGIAARDGRLRPGDQILEVNGEELAHVTHLQACLMLS--SLTGPVCRLTVYREQAVEAGHSLSSPASQHRIDAKQELLRIVLPKSQEKRLGIKLAGKRNCLGLYVLGLVPDGQAQLDGRLRKDDRILEINGVDLSNGTQEQAAQLIADAKDQVTLVILRQVRPQTPDIIRAASGENVSKCVVFSTSGQQSTVASPAPVRAITCTTTVNPPPPPRGITTLSNVPLEPDVQLAAGARVCQERVLVLTKRPGESLGMSVAGGVASQRGDVPIYVTNLAPNGLVARSGRVFRGDILLAVNDIELLGLSHERAVEALKNARDTCTQVTLRLLKGPECSL--EDRNFIPSWLFWLQLPRYCQIPRLVVLVRDPVIG-LGFSIIGGND-FFPDANETVTNTTAKETDGPDGTRKTPTPRPIVIKSIVPGSPCYWDGRLKCGDILLSVDHYPLTNISHAHAVALLKHCTGEVKLRIVSWPGTIV 987
BLAST of Ligand of Numb protein X 2 vs. TrEMBL
Match: A0A0X3P7T5 (Ligand of Numb protein X 2 (Fragment) OS=Schistocephalus solidus OX=70667 GN=LNX2 PE=4 SV=1) HSP 1 Score: 458.373 bits (1178), Expect = 5.427e-146 Identity = 280/675 (41.48%), Postives = 385/675 (57.04%), Query Frame = 3
Query: 171 DRLMSVTVTSSPCQEFIPKSEIDPNLIKQCQGLFVSCCYSTAGCTFKAVGREVAAHEWQCPYGQKSPEX-------XXXXXXXXXXXXVFQTDAAGVSINGSQDISNTNSGNNFPKPQDCFYQNNSFYTPLNHLDNLEFN---------VEDFTITIIN-----PERLEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWL---------------PVAKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAG----TEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFD--SPISFKS----TYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGN-----DPIYPFNTNSSLTDLSHNK-------------EAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
D+L+ + + C E + + +++ +L C+G + C + GC F+A H +C + +S + S+ V QT S GS S G P P Q+ + +N + + + D +T I ERL G+SFVGG DTPL C+VIQEI+ DG +A+DGRLRPGDQI + N L+QA+H + R +LS I+ S LT++R+ P +EEIL L K D+++G+KL GK N GLYI V+PDS+A LDGRL KDDRIL+IN DL GTQ+EA II+S + V V++R++RPQTPDIIR A + ++ T + P L VG + + SP S KS RE+V+ ++K+PGESLGM+VAGGV SQRGDTPVY+T+ P S++ + KGD+LLAVNE ELLGLSHERAVEALK R C+KVT+R+L+ P NF+PSW+YWL+LP YCQIP+ I LSR +A+GS GFSIVGGN DP P T + LT+ S + + +PI++KSIV G+PAH++GRLKCGD+++++N FSL++VSH+ AV L K+ G V ++VVSWPGTI+
Sbjct: 237 DKLLVICPNTEHCDEVLCRCDLESHLTYWCRGSVIPCLNAKTGCQFRAARALHTEHRRECRFQTRSTTANFSGGGGGGIVGPGSAFDVVHQTSPVVRSSTGSGAKSAVRFG---PMPSRRAAQDQTGRADVNASSLVVGSKPRTRSVSPIRDCEVTTIELPWQPNERL--GISFVGGSDTPLLCVVIQEIYLDGLVALDGRLRPGDQILETNGVDLTQATHHQARVLLSSITAS-RPCTLTVYRERASSETGSLNSGKLSSTATGDNPFEREEILHINLLKRLDKRLGIKLVGKKNLPGLYILGVVPDSEADLDGRLHKDDRILEINGVDLREGTQDEAANIIRSAGEHVSLVVARVIRPQTPDIIRTASGDIVIDYGADCTATTAGAGPPALVPPV-----VGPATLSLGAISPPSLKSPSTRLCREQVLVINKRPGESLGMTVAGGVASQRGDTPVYVTDFDPGSVVGRMGRLSKGDVLLAVNEVELLGLSHERAVEALKSTRKTCSKVTLRLLEGPE--TSTAKGNFLPSWLYWLQLPRYCQIPKTIVLSRTSASGSFGFSIVGGNNSMSADPASP--TMTVLTNASQREVTRPSATSSSSCVSSSPQPIVVKSIVPGTPAHRDGRLKCGDLILAVNQFSLADVSHAYAVALLKNCVGDVQIRVVSWPGTIV 896
BLAST of Ligand of Numb protein X 2 vs. TrEMBL
Match: A0A564XXP6 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS355 PE=4 SV=1) HSP 1 Score: 449.899 bits (1156), Expect = 9.664e-145 Identity = 269/678 (39.68%), Postives = 378/678 (55.75%), Query Frame = 3
Query: 171 DRLMSVTVTSSPCQEFIPKSEIDPNLIKQCQGLFVSCCYSTAGCTFKAVGREVAAHEWQCPYGQKSPEXXXXXXXXXX------------------XXXVFQTDAAGVSINGSQDISNTNSGNNFPKPQDCFYQNNSFYTPLNHLDNLEFNVEDFTITIINPERLEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQS-GDTIYLTIHRDIVDKRWL------------------------PVAKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDS---PISFKSTY----REEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGN----------DPIYPF-------NTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
D+L+ + S C E + + +++ +L+ C+G V C + GC F+A + H +C + ++P ++ +++ S T+A +S GS T S P +H+ + E TI + G+SFVGG DTPL CIVIQE++ DG +AMDGRLRPGDQI + N L+QA+H + R L+ + + LT++R+ R L P +EEIL L K +D+++G+KL GK N G+YI ++PDS+A LDGRL KDDRIL+IN DL GTQ EA II++ ++ V V++R++RP TP+++R+ E + SS +A+TR P + P S KS RE+ + V+K PGESLGM+VAGGV SQRGDTPVYITN+ P +L + KGD+LLAVNE ELLGLSHE+AVEALK R +KVT+RVL+ P + +NF+PSW+YWL+LP YCQIPR I L R A GSLGFSIVGGN P+ F + + S T S + + +PI++KSIV GSPAH++GRLKCGD+++++N + L++VSH+ AV L K G VH++VVSWPGTI+
Sbjct: 69 DKLLVICPNSEHCNEVLSRCDLESHLVYWCRGTVVPCSNAKTGCQFRAARALQSEHRRECRFQPRTPGTTHVAVSGGSPIVRPIATKSVVRFGPMPAVRRVATEAPLMSAEGSAFEETTPS----PPTTASSIGGGGGVRSASHIRDCEVT----TIELPWKPNASLGISFVGGSDTPLLCIVIQEVYLDGIVAMDGRLRPGDQILEANGIDLTQATHHQARTALTSVGGGVARSFELTVYRE----RALVSECGGSSSSGSSGRRRFGSTPENPFEREEILQVTLMKRSDKRLGIKLVGKKNLPGVYILGLVPDSEADLDGRLNKDDRILEINGIDLREGTQEEAANIIRTAEEHVALVVARVIRPHTPEMLRSGSGEIFMDFGGGGNGSSSTTSTSAATRSPGTAVPPPATSASVLPSSLKSPSTRLCREQEITVEKMPGESLGMTVAGGVASQRGDTPVYITNLVPTGVLGRAGQLAKGDVLLAVNEIELLGLSHEKAVEALKATRKTSSKVTIRVLEGPE--TSSGRENFLPSWLYWLQLPRYCQIPRTIILPR-TANGSLGFSIVGGNTAAGSDDSGTSPLTAFSRSVDFESRHPSATHGSSSVTSSPQPIVVKSIVPGSPAHRDGRLKCGDLILAVNQYPLTDVSHAHAVALLKHCVGDVHIRVVSWPGTIV 731
BLAST of Ligand of Numb protein X 2 vs. Ensembl Cavefish
Match: lnx2a (ligand of numb-protein X 2a [Source:ZFIN;Acc:ZDB-GENE-060228-2]) HSP 1 Score: 251.906 bits (642), Expect = 1.931e-73 Identity = 171/499 (34.27%), Postives = 261/499 (52.30%), Query Frame = 3
Query: 585 ITIINPER----LEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRD---------IVDKRWLPVAKEEILTFVLEK-PTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIA-TSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPN---------LLLMANTD---NFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTI 2000
IT I R +E G+S VGG +TPL IVIQE++RDG IA DGRL GDQI ++N +S H R L R T+ LT+ R+ + P + L K + ++G+KL +T+++G+++ ++L A+ DGRL+ +DR+L +NEHDL GT +A +II++ + V +ISR + QT + + + P+ T++ P L T +E+ + V K+P ESLGM+VAGG S+ G+ P+++T++ P+ L I +GD+LL++N +L LSH AV LK + + + V +R L+V LL +D ++ PSW+ WL LP Y I L R + GS GFSIVGG Y N H+ +A IK+IV G+PA+ +GRLKCGD+++++N S + +SHS V + K + + L VVSWPG++
Sbjct: 222 ITTIEVHRSNPYVELGISIVGGNETPLINIVIQEVYRDGVIARDGRLLAGDQILQVNNVDISNMPHSFARSTLGRPCA---TLQLTVLRERRCTARPPAVAPTPSSPEGSPASMRITLHKRESTEQLGIKLVRRTDEAGVFVLDLLDGGLAAKDGRLRSNDRVLAVNEHDLRHGTPEQAAQIIQASGERVNLLISRPAK-QTMAVHTGRESWCHEHFLPLPRTAAPSPDLSQC---------------------VTCKEKHITVKKEPHESLGMTVAGGRGSKSGELPIFVTSVQPHGCLSRDGRIKRGDVLLSINGQDLTCLSHSEAVGTLKSSAAS-SSVQLRALEVSTVDEPGHDEGLLPHHESDYDASWSPSWVMWLGLPSYLHSSHEIILRR-SHPGSWGFSIVGG----YEEN---------HSNQA----FFIKTIVLGTPAYYDGRLKCGDMIVAVNGLSTAGMSHSALVPMLKEQRSRLALTVVSWPGSL 676
BLAST of Ligand of Numb protein X 2 vs. Ensembl Cavefish
Match: lnx1 (ligand of numb-protein X 1 [Source:NCBI gene;Acc:103042826]) HSP 1 Score: 249.98 bits (637), Expect = 6.976e-71 Identity = 175/543 (32.23%), Postives = 269/543 (49.54%), Query Frame = 3
Query: 522 NSFYTPLNHLDNLEFNVEDFTITIINPERLE----FGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWL-----------------PVAKEEILTFVLEKPT-DRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAA--GTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKV-------------------------PNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
NS + L L L + D +T + R + +S VGG +TPL I+IQ+I+R+G IA DGRL PGD I K+N ++ H L+ + Q + LT+ R+ + P+ +++ L VL K D ++G+KL + ++ G++I +L A+ D RL+ DDR+L IN HDL +G A +I++ + V F++SR PDI++ A E +P+ + DS K E+ V + K+P ESLGM+VAGG+NS+ D PVYITNI PN ++ I KGDILL VN +L G++ AV LK + + V +RVL++ ++ + +D++ P W+ WL+LP + + I L R + +GSLGFSIVGG + I N N S I+SIV G+PA+ +GR++CGDIL+ +N S ++H+ V L K +G + L +VSWPG+++
Sbjct: 451 NSKKSELFALPQLHHLIPDGEVTTVKITRADPCEPLAISIVGGNETPLVRILIQDIYREGVIARDGRLLPGDMILKVNGIDITNVPHCY---ALAALKQPCTLLRLTVLREQRHRYRSHHHHHTPSPGELYPGHGPMIRDDSLHVVLVKGAPDEQLGIKLVRRPDEHGVFIFHLLEGGLAARDARLRVDDRVLAINGHDLRYGAPEHAALLIQASVERVHFIVSRQTHVPAPDILQEAPWNMEGPPPYSPVDIEHTL-------------------LDS--CHKPACYEKTVTLSKEPNESLGMTVAGGMNSRGWDLPVYITNIDPNGVVSQEGSIRKGDILLNVNGVDLTGVTRGEAVANLK---NTSSPVVLRVLEMRPPDVSSLDCMPPLHSPCSLSPCSPSDIKMPLPSDDYSPLWVSWLQLPRHLYCCKDIVLRR-STSGSLGFSIVGGQEEI---NCNQSF--------------FIRSIVEGTPAYNDGRIRCGDILLEVNGKSTWGMTHTALVRLLKELRGRITLTIVSWPGSLL 948
BLAST of Ligand of Numb protein X 2 vs. Ensembl Cavefish
Match: lnx1 (ligand of numb-protein X 1 [Source:NCBI gene;Acc:103042826]) HSP 1 Score: 249.595 bits (636), Expect = 1.409e-70 Identity = 181/568 (31.87%), Postives = 281/568 (49.47%), Query Frame = 3
Query: 438 VSINGSQDISNTNSGNNFPKPQDCFYQNNSFYTPLNHLDNLEFNVEDFTITIINPERLE-FGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWL-----------------PVAKEEILTFVLEKPT-DRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAA--GTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKV-------------------------PNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
V+ +G+++ N + N PQ+ F L L +L + E T+ I + E +S VGG +TPL I+IQ+I+R+G IA DGRL PGD I K+N ++ H L+ + Q + LT+ R+ + P+ +++ L VL K D ++G+KL + ++ G++I +L A+ D RL+ DDR+L IN HDL +G A +I++ + V F++SR PDI++ A E +P+ + DS K E+ V + K+P ESLGM+VAGG+NS+ D PVYITNI PN ++ I KGDILL VN +L G++ AV LK + + V +RVL++ ++ + +D++ P W+ WL+LP + + I L R + +GSLGFSIVGG + I N N S I+SIV G+PA+ +GR++CGDIL+ +N S ++H+ V L K +G + L +VSWPG+++
Sbjct: 489 VANDGTEERDNLRNAN---IPQEVFA--------LPQLHHLIPDGEVTTVKITRADPCEPLAISIVGGNETPLVRILIQDIYREGVIARDGRLLPGDMILKVNGIDITNVPHCY---ALAALKQPCTLLRLTVLREQRHRYRSHHHHHTPSPGELYPGHGPMIRDDSLHVVLVKGAPDEQLGIKLVRRPDEHGVFIFHLLEGGLAARDARLRVDDRVLAINGHDLRYGAPEHAALLIQASVERVHFIVSRQTHVPAPDILQEAPWNMEGPPPYSPVDIEHTL-------------------LDS--CHKPACYEKTVTLSKEPNESLGMTVAGGMNSRGWDLPVYITNIDPNGVVSQEGSIRKGDILLNVNGVDLTGVTRGEAVANLK---NTSSPVVLRVLEMRPPDVSSLDCMPPLHSPCSLSPCSPSDIKMPLPSDDYSPLWVSWLQLPRHLYCCKDIVLRR-STSGSLGFSIVGGQEEI---NCNQSF--------------FIRSIVEGTPAYNDGRIRCGDILLEVNGKSTWGMTHTALVRLLKELRGRITLTIVSWPGSLL 1000
BLAST of Ligand of Numb protein X 2 vs. Ensembl Cavefish
Match: lnx1 (ligand of numb-protein X 1 [Source:NCBI gene;Acc:103042826]) HSP 1 Score: 249.595 bits (636), Expect = 1.409e-70 Identity = 181/568 (31.87%), Postives = 281/568 (49.47%), Query Frame = 3
Query: 438 VSINGSQDISNTNSGNNFPKPQDCFYQNNSFYTPLNHLDNLEFNVEDFTITIINPERLE-FGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWL-----------------PVAKEEILTFVLEKPT-DRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAA--GTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKV-------------------------PNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
V+ +G+++ N + N PQ+ F L L +L + E T+ I + E +S VGG +TPL I+IQ+I+R+G IA DGRL PGD I K+N ++ H L+ + Q + LT+ R+ + P+ +++ L VL K D ++G+KL + ++ G++I +L A+ D RL+ DDR+L IN HDL +G A +I++ + V F++SR PDI++ A E +P+ + DS K E+ V + K+P ESLGM+VAGG+NS+ D PVYITNI PN ++ I KGDILL VN +L G++ AV LK + + V +RVL++ ++ + +D++ P W+ WL+LP + + I L R + +GSLGFSIVGG + I N N S I+SIV G+PA+ +GR++CGDIL+ +N S ++H+ V L K +G + L +VSWPG+++
Sbjct: 489 VANDGTEERDNLRNAN---IPQEVFA--------LPQLHHLIPDGEVTTVKITRADPCEPLAISIVGGNETPLVRILIQDIYREGVIARDGRLLPGDMILKVNGIDITNVPHCY---ALAALKQPCTLLRLTVLREQRHRYRSHHHHHTPSPGELYPGHGPMIRDDSLHVVLVKGAPDEQLGIKLVRRPDEHGVFIFHLLEGGLAARDARLRVDDRVLAINGHDLRYGAPEHAALLIQASVERVHFIVSRQTHVPAPDILQEAPWNMEGPPPYSPVDIEHTL-------------------LDS--CHKPACYEKTVTLSKEPNESLGMTVAGGMNSRGWDLPVYITNIDPNGVVSQEGSIRKGDILLNVNGVDLTGVTRGEAVANLK---NTSSPVVLRVLEMRPPDVSSLDCMPPLHSPCSLSPCSPSDIKMPLPSDDYSPLWVSWLQLPRHLYCCKDIVLRR-STSGSLGFSIVGGQEEI---NCNQSF--------------FIRSIVEGTPAYNDGRIRCGDILLEVNGKSTWGMTHTALVRLLKELRGRITLTIVSWPGSLL 1000
BLAST of Ligand of Numb protein X 2 vs. Ensembl Cavefish
Match: lnx2a (ligand of numb-protein X 2a [Source:ZFIN;Acc:ZDB-GENE-060228-2]) HSP 1 Score: 241.121 bits (614), Expect = 9.054e-69 Identity = 167/493 (33.87%), Postives = 259/493 (52.54%), Query Frame = 3
Query: 585 ITIINPER----LEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRD---------IVDKRWLPVAKEEILTFVLEK-PTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPI---ATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPN---------LLLMANTD---NFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLK 1976
IT I R +E G+S VGG +TPL IVIQE++RDG IA DGRL GDQI ++N +S H R L R T+ LT+ R+ + P + L K + ++G+KL +T+++G+++ ++L A+ DGRL+ +DR+L +NEHDL GT +A +II++ + V +ISR + QT + + + P+ A S P L+ + + + +S T +E+ + V K+P ESLGM+VAGG S+ G+ P+++T++ P+ L I +GD+LL++N +L LSH AV LK + + + V +R L+V LL +D ++ PSW+ WL LP Y I L R + GS GFSIVGG Y N H+ +A IK+IV G+PA+ +GRLKCGD+++++N S + +SHS V + K + + L+
Sbjct: 268 ITTIEVHRSNPYVELGISIVGGNETPLINIVIQEVYRDGVIARDGRLLAGDQILQVNNVDISNMPHSFARSTLGRPCA---TLQLTVLRERRCTARPPAVAPTPSSPEGSPASMRITLHKRESTEQLGIKLVRRTDEAGVFVLDLLDGGLAAKDGRLRSNDRVLAVNEHDLRHGTPEQAAQIIQASGERVNLLISRPAK-QTMAVHTGRESWCHEHFLPLPRTAAPSPAPSLQLSRSSTHR----------DLSQCVTCKEKHITVKKEPHESLGMTVAGGRGSKSGELPIFVTSVQPHGCLSRDGRIKRGDVLLSINGQDLTCLSHSEAVGTLKSSAAS-SSVQLRALEVSTVDEPGHDEGLLPHHESDYDASWSPSWVMWLGLPSYLHSSHEIILRR-SHPGSWGFSIVGG----YEEN---------HSNQA----FFIKTIVLGTPAYYDGRLKCGDMIVAVNGLSTAGMSHSALVPMLKEQRSRLALQ 727
BLAST of Ligand of Numb protein X 2 vs. Ensembl Sea Lamprey
Match: LNX2 (ligand of numb-protein X 2 [Source:HGNC Symbol;Acc:HGNC:20421]) HSP 1 Score: 149.058 bits (375), Expect = 5.933e-40 Identity = 117/369 (31.71%), Postives = 183/369 (49.59%), Query Frame = 3
Query: 954 SGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGI--DKGDILLA-VNETELL----GLSHERAVEALKLARDNC----NKVTVRVLKVPNLLLMANTDNFIPSW--MYWLK-LPGYCQI-----PRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
SG+++ ++L A++DGRL+ DR+L IN HDL GT A ++I+ +D V V+SR +P P G S+ P A S P P + +S + +E+VV V K+ ESLGM+VAGG+ S+ G+ P+++T++ + L I D G + L V+E + GL H+ + + K ++ + V + + LL+ ++ S+ W L YC R + R + +GS GFSIVGG + N S +P IKSIV G+PA+ +GRLKCGD+++++N + +SH+ V L K +G V L VVSWPG+I+
Sbjct: 9 SGVFVLDLLEGGPAAVDGRLRPSDRVLSINGHDLRQGTPEMAAQVIQGSEDRVHIVVSRPGKPGPPGPGGQDG---RSHAIPGAAHPSPP--------------KPCHYRE-LSQAVSCQEKVVTVRKEASESLGMTVAGGLASRSGELPIFVTSVQGHGCLGRDGRIHRDMGSVYLHPVSEIKACRLGTGLQHKGQLASFKKTHNSSIVKLSLVEIWITSEHAALLLNLPKSYYRSFNGRTWFSILYSYCASGSFLQSRKEVILRRSTSGSWGFSIVGG------YEENHS-----------NQPFFIKSIVLGTPAYNDGRLKCGDMIVAVNGHGTTGMSHAALVTLLKELRGKVTLTVVSWPGSIL 342
BLAST of Ligand of Numb protein X 2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004677.1 (pep scaffold:Pmarinus_7.0:GL479538:28591:32639:-1 gene:ENSPMAG00000004260.1 transcript:ENSPMAT00000004677.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 87.8113 bits (216), Expect = 2.235e-20 Identity = 53/180 (29.44%), Postives = 91/180 (50.56%), Query Frame = 3
Query: 660 IVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVAKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTP 1199
I+I E++ +G A DGRL GDQI K+N L A+H D ++ + S + L ++RD R + + T L K R +GL + GK + +G+++ EV+ A DGRL + D+IL ++ D+ +Q I+K + VK + R+ + ++ + T S ++P
Sbjct: 4 IMIHEVYEEGAAAKDGRLWAGDQILKVNGTDLRSATH---EDAINALRASPQLVRLYVYRDEARYRGDDESALHVFTVELLKKAGRGLGLSIVGKKSGNGVFVSEVVHGGAAEADGRLTQGDQILSVDGEDVRSASQETVAAILKCAQGVVKMEVGRM---KVGSLLSSRRTSDTSQVSP 177
BLAST of Ligand of Numb protein X 2 vs. Ensembl Sea Lamprey
Match: LIN7B (lin-7 homolog B, crumbs cell polarity complex component [Source:HGNC Symbol;Acc:HGNC:17788]) HSP 1 Score: 55.0694 bits (131), Expect = 4.430e-9 Identity = 32/79 (40.51%), Postives = 46/79 (58.23%), Query Frame = 3
Query: 1338 KPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVR 1574
K E LG +V GG + ++P+YI+ I P + G+ +GD LL+VN + G HERAVE LK A+ + K+ VR
Sbjct: 75 KTDEGLGFNVMGG---KEQNSPIYISRIIPGGVADRQGGLKRGDQLLSVNGVSVEGEHHERAVELLKQAQGSV-KLVVR 149
BLAST of Ligand of Numb protein X 2 vs. Ensembl Sea Lamprey
Match: lin7a (lin-7 homolog A (C. elegans) [Source:ZFIN;Acc:ZDB-GENE-040426-1665]) HSP 1 Score: 53.9138 bits (128), Expect = 1.344e-8 Identity = 33/85 (38.82%), Postives = 49/85 (57.65%), Query Frame = 3
Query: 1320 VVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVR 1574
VVE+ K E LG ++ GG + ++P+YI+ I P + G+ +GD LL+VN + G HE+AVE LK A+ K+ VR
Sbjct: 80 VVEL-PKTDEGLGFNIMGG---KEQNSPIYISRIIPGGVADRQGGLKRGDQLLSVNGVSVEGEHHEKAVELLKQAQGTV-KLVVR 159
BLAST of Ligand of Numb protein X 2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002205.1 (pep scaffold:Pmarinus_7.0:GL477144:51012:68257:1 gene:ENSPMAG00000001988.1 transcript:ENSPMAT00000002205.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 54.6842 bits (130), Expect = 5.853e-8 Identity = 58/214 (27.10%), Postives = 92/214 (42.99%), Query Frame = 3
Query: 921 MGLKLAGK-TNKSGL---YIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVK--DSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRG--DTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALK 1538
+G L + T SGL YI+ +LP A DGRL DR++ +N D+ +Q++ + I++ V SV ++SR D+ +TP A + + + G T P + E+ + P LG+SV G S+ G D +Y+ +I + GD L+AVN LLG S+ A+E L+
Sbjct: 397 LGFSLTSRDTPVSGLGHVYIKHILPRGAAIADGRLHAGDRLIMVNGVDVSSMSQDQVVSILRGVHPGSSVDLLVSR----------------HDALLTPPAQEARLVKQMHPDQQAERSGGMIVTASGPCECLNL---EIALSECGPA-GLGVSVKGN-RSRDGAHDLGIYVKSIIAGGAAAKDGRLRIGDQLVAVNREPLLGRSNAAAMETLR 589
BLAST of Ligand of Numb protein X 2 vs. Ensembl Nematostella
Match: EDO33706 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SRG3]) HSP 1 Score: 91.6633 bits (226), Expect = 5.630e-19 Identity = 129/542 (23.80%), Postives = 217/542 (40.04%), Query Frame = 3
Query: 549 LDNLEFNVEDF--TITIINPERLEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRW----------LPVAKEE--ILTFVLEKPTDRKMGLKLAG--KTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGT-----------------EPDSNMT---------PI-----------ATSSSYPGLKNASTRGYEV----GNSPFTFDSPISFKSTYREEVVEVDKKPGE-----------SLGMSVAG--GVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGND----PIYPFNTNSSLTDLS-HNKEAPYRPIMIKSIVSGSPAH-----KNGRLKCGDILISINDFSLSNVSHSDA 1934
LDNL +V+ TITI + GVS G D V+ + R G IA+DGR+ GD I +N+ L S R VL + S D I ++I + R +P + E + L + + +G+ +AG + + G++++ VL +S + G LK D+IL+++ HDL + EA+E+I+ + V+FV+ I + P + G+ EP +T PI ++S +P L+ A+ + ++P + P S++ RE + GE LG+S+AG GV R ++I ++ + I + D +L VN T + G+SH +A LK + R + L S +K P Q T + G + G P+ P + SL S P ++ + G K+GRL+ GD LI+++D ++ +SH++
Sbjct: 1105 LDNLPDSVKSRMQTITIRRQMVGKLGVSLKGDEDG--SGCVVTSVMRGGAIAIDGRIGVGDHIVAVNDESLIGLSRHAARAVLRKQSLQKD-IVVSIDKQEASTREDSPAFLTPFDVPFVETESGLRVVELRREPEVGLGISIAGNKRGQRQGVHVRHVLENSSVARLGELKAGDQILEVDGHDLRNASHEEAVEVIRRARSPVRFVVRTIQDKEDPLSLDTMGSPSSFEGPVTTINARTSHEPSQPITARDDLHKDIPISAKNLDSPPRDSSSRDFPSLEQAAPHKEQTIEANVSAPDHSEMP-SYEDRIRELQAKYPDVTGEHLLVSLSKGNTGLGLSIAGGKGVAVNR----IFIVDVKSGGPAEQDGRIKQADEILEVNRTPVRGMSHYQASTVLKNTGTSVELALGRSREAAEYLSRTRQQASPHSTEPDIK-PSNIQ-----TTESTPSISPRGVEVAAGPRRTPPPVAPKPSTDSLKRSSPQAGSVTVSPTLLAELSGGQLVEYIDFVKDGRLRKGDQLIAVDDQAIVGLSHAEG 1632 HSP 2 Score: 73.9442 bits (180), Expect = 1.841e-13 Identity = 108/432 (25.00%), Postives = 172/432 (39.81%), Query Frame = 3
Query: 921 MGLKLAGKTNKS--GLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRP-----QTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSV--AGGVNSQRGDTPVYITNIAPNSLLK-----NTNGIDKGDILLAVNETELLGLSHERAV-------EALKLARDN--------CNKVTVRVL------------------KVPNLLLMANT----DNFIPSWMYWLKLPGYCQIPRMIT----LSRDAATGS-----------------------LGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVV 1982
+GL +AG + ++I +V A DGR+K+ D IL++N + + +A ++K+ SV+ + R +T TEPD + I T+ S P + S RG EV P P++ K + +SL S AG V +P + ++ L++ + KGD L+AV++ ++GLSH E A+D + T RVL +P L + A + +N P Y L +P + + T + +D+ + S LGFSIVGG+ S DL PI +K++ A ++GRLK GD +I++N SL VSH AV+ K T+G + L V+
Sbjct: 1437 LGLSIAGGKGVAVNRIFIVDVKSGGPAEQDGRIKQADEILEVNRTPVRGMSHYQASTVLKNTGTSVELALGRSREAAEYLSRTRQQASPHSTEPDIKPSNIQTTESTPSI---SPRGVEVAAGPRRTPPPVAPKPST------------DSLKRSSPQAGSVTV----SPTLLAELSGGQLVEYIDFVKDGRLRKGDQLIAVDDQAIVGLSHAEGTIVIHEVYEDGAAAKDGRLWAGDQILERTTGRVLLNVARLKPGNSSRPSSLSSLPQLQIGAESTSSIENSFPPVNYDLDIPSEPEANQRATPPLPVQQDSPSQSDDEEESESPLQTKIIELERGPEGLGFSIVGGH--------GSPHGDL---------PIYVKTVFPTGAASRDGRLKRGDQIIAVNGQSLVGVSHESAVSQLKKTRGKIILTVL 1832 HSP 3 Score: 57.3806 bits (137), Expect = 2.067e-8 Identity = 56/241 (23.24%), Postives = 110/241 (45.64%), Query Frame = 3
Query: 879 EILTFVLEKPTDRKMGLKLAGKTNKS----GLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFG-TQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYRE-EVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLK 1583
E+ T VL KP +G + G +++ G++IQE+ + A DGRL++ D+IL I+ L G + EA+ +++ + V+ +++R P+T +G + TP S+ + +AST P + +R+ E +++ G LG + GG +S V I I P + + + D ++ + + + G+ E+ + L+ A + V R+++
Sbjct: 161 EVETVVLNKPGQGGLGFSVVGLKSENRGELGIFIQEIQEEGVAGRDGRLRESDQILSIDGQQLDSGISHEEAIVLLQKTRGEVELIVARGGIPRTGTSRTTSGASSVISRTP----SNVSLVSDASTT------------IPADDGTHWRQIETIDLHND-GTGLGFGIIGGRSSG-----VSIKTILPGGVADKDGRLQEHDQIMQIGDVNVGGMGSEQVAQVLRDAGSHVRLVISRLVE 379 HSP 4 Score: 53.9138 bits (128), Expect = 2.624e-7 Identity = 83/385 (21.56%), Postives = 153/385 (39.74%), Query Frame = 3
Query: 951 KSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRP-QTPDIIRAAGTE----PDSNMTPIAT----SSSYPG------LKNASTRGYEVG---------NSPFTFDSPISFKS------------TYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALK---LARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIP---RMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKV 1979
K+ + I+ ++ A DG L DR++ +NE +L + + A++ +K VI + +P PD + PD+ + T + S PG + N G + +S F F P+ F R + + + ++ LG+S+ G + + +T++ + I GD ++AVN+ L+GLS A L+ L +D + V + K A+T P+++ +P + + R++ L R+ G LG SI G NK + + ++ ++ S + G LK GD ++ ++ L N SH +AV + + + PV V
Sbjct: 961 KTVIVIRSLVHGGVAEQDGSLHPGDRLMSVNEVNLEHASLDFAVQTLKGTNRG--NVIIGVAKPIPVPDDYEEDSNQGESLPDTTRPFLPTFDLDNESRPGKADISLMDNIENNGKDSDTDSMVTSEQSSKFDFGPPLLFSGDSLDLDNLPDSVKSRMQTITIRRQMVGKLGVSLKGDEDG----SGCVVTSVMRGGAIAIDGRIGVGDHIVAVNDESLIGLSRHAARAVLRKQSLQKD----IVVSIDK-----QEASTREDSPAFLTPFDVP-FVETESGLRVVELRREPEVG-LGISIAG-------------------NKRGQRQGVHVRHVLENSSVARLGELKAGDQILEVDGHDLRNASHEEAVEVIRRARSPVRFVV 1309
BLAST of Ligand of Numb protein X 2 vs. Ensembl Nematostella
Match: EDO45624 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RSE9]) HSP 1 Score: 80.1073 bits (196), Expect = 1.604e-16 Identity = 73/308 (23.70%), Postives = 134/308 (43.51%), Query Frame = 3
Query: 615 FGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVAKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALK 1538
G+ +GG DTPL + I++I G LR GDQ+ ++N+ L +H +VL + + LT+ R + P ++ T L+K + +G+ ++G + + ++ V+P A+ DGR++K DR+L +N T E L +++++ V+ +G + + + P +K + E G S F SV GG +S GDTP+YI + +S + +G++ GD +L VN + G+++ A+EA++
Sbjct: 9 IGLMVIGGLDTPLGMLYIKDIQPGTPAEKCGHLRTGDQLLQVNDECLVGVTHAYALEVLK---NTPPLVKLTVAR-----KKDPDRDSDVFTVELKKDSKGSLGIHVSGGVGTNCIDVRHVVPLGVAAKDGRIRKGDRVLSVNGRSTKGLTHQEVLNLLQNLPRRVRLF---------------SGGNGKAFKEILQKTPPEPSVKEIT---LEKGPSGLGF---------------------------SVGGGRDSLYGDTPIYIKYVFKDS-ASSRSGLEIGDEVLEVNGRHMRGMTNVEALEAIR 262 HSP 2 Score: 53.1434 bits (126), Expect = 1.411e-7 Identity = 54/205 (26.34%), Postives = 93/205 (45.37%), Query Frame = 3
Query: 1338 KPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKS 1952
K + +G+ V GG+++ G +YI +I P + + + GD LL VN+ L+G++H A+E LK K+TV K P+ ++D F + L +D + GSLG + GG TN I ++ +V A K+GR++ GD ++S+N S ++H + +NL ++
Sbjct: 4 KEDQGIGLMVIGGLDTPLG--MLYIKDIQPGTPAEKCGHLRTGDQLLQVNDECLVGVTHAYALEVLK-NTPPLVKLTVARKKDPD----RDSDVF------------------TVELKKD-SKGSLGIHVSGG------VGTNC---------------IDVRHVVPLGVAAKDGRIRKGDRVLSVNGRSTKGLTHQEVLNLLQN 161
BLAST of Ligand of Numb protein X 2 vs. Ensembl Nematostella
Match: EDO46059 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RR65]) HSP 1 Score: 72.0182 bits (175), Expect = 2.368e-14 Identity = 67/226 (29.65%), Postives = 101/226 (44.69%), Query Frame = 3
Query: 1317 EVVEVD-KKPGESLGMSVAGGVNSQR--GDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVT-VRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPY-RPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKV 1979
E +E+ ++ G LG S+AGG ++ D +YIT + P + D +L+VN T + + H++AV+ALK A ++VT VR K N P + + LGFSI GG S N+ P I I I+ G A ++GRL+ GD +IS+N +L +VSH DAV + K+T+ V + V
Sbjct: 3 ETLEITLERGGSGLGFSIAGGTDNPHVDDDFGIYITKVIPGGAAAQDGRLQVNDCILSVNGTNTVDIEHQQAVDALKAAG---SQVTLVREKKRGN-------------------------APTREEIVLIKGSKGLGFSIAGG----------------SGNQHVPGDNGIYITKIIEGGAAQQDGRLQVGDKIISVNLQNLEDVSHEDAVQVLKATKERVTIVV 184 HSP 2 Score: 65.4698 bits (158), Expect = 3.231e-12 Identity = 68/241 (28.22%), Postives = 102/241 (42.32%), Query Frame = 3
Query: 879 EILTFVLEKPTDRKMGLKLAGKTNKS------GLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQR--GDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRV 1577
E L LE+ +G +AG T+ G+YI +V+P A+ DGRL+ +D IL +N GT +E Q D ++AAG S +T + R + GN+P REE+V + K + LG S+AGG +Q GD +YIT I + + GD +++VN L +SHE AV+ LK ++ V R+
Sbjct: 3 ETLEITLER-GGSGLGFSIAGGTDNPHVDDDFGIYITKVIPGGAAAQDGRLQVNDCILSVN------GTNTVDIE-----------------HQQAVDALKAAG----SQVTLV--------------REKKRGNAP------------TREEIVLI--KGSKGLGFSIAGGSGNQHVPGDNGIYITKIIEGGAAQQDGRLQVGDKIISVNLQNLEDVSHEDAVQVLKATKERVTIVVSRL 187 HSP 3 Score: 61.6178 bits (148), Expect = 7.441e-11 Identity = 51/183 (27.87%), Postives = 88/183 (48.09%), Query Frame = 3
Query: 615 FGVSFVGGFDTPLQ----CIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVAKEEILTFVLEKPTDRKMGLKLAGKTNK------SGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRI 1133
G S GG D P I I ++ G A DGRL+ D I +N + IE + + + +G + L + KR +EEI VL K + + +G +AG + +G+YI +++ A DGRL+ D+I+ +N +L + +A++++K+ K+ V V+SR+
Sbjct: 16 LGFSIAGGTDNPHVDDDFGIYITKVIPGGAAAQDGRLQVNDCILSVNG---TNTVDIEHQQAVDALKAAGSQVTLVREK----KRGNAPTREEI---VLIKGS-KGLGFSIAGGSGNQHVPGDNGIYITKIIEGGAAQQDGRLQVGDKIISVNLQNLEDVSHEDAVQVLKATKERVTIVVSRL 187
BLAST of Ligand of Numb protein X 2 vs. Ensembl Nematostella
Match: EDO33432 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SS78]) HSP 1 Score: 71.633 bits (174), Expect = 8.589e-13 Identity = 91/374 (24.33%), Postives = 152/374 (40.64%), Query Frame = 3
Query: 915 RKMGLKLAG-------KTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISR----IVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNS---QRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALK---------LARDNC--NKVT--VRVLKVPNLLLMANTDNFIPSWMYWLKL----PGYCQIPRMITLSRDAATGSLGFSIVGGNDPI-YPFNTNSSLTDLSHNKEAPYRPIMIKSIV-SGSPAHKNGRLKCGDILISINDFSLSNVSHSDAV 1937
+ +G+ +AG K N G++I + + A DG L D+ILK+N D+ T ++A++++KS + + R I + P + + + T + + P +++ TR +E + + + + LG S+AGG S + GD ++I+ IA + + + GD +L++N ++ H+ AV L + RD K+T R K N + PS K P +I I L + LGFSIVGG+D +PF + I I IV +G A N LK GD ++ +N + N +H DAV
Sbjct: 576 KGLGINIAGGKGSTPYKENDEGIFISRISENGPAGRDGILHVGDKILKVNGVDISNATHHQAVDVLKSTGKDITLYVVREKQEIEKRTVPKTAKDESVKEEPKKTGVRFAPE-PEMEDIETR--------------------PEKETITLKRGGDKGLGFSIAGGKGSTPYKDGDPGIFISKIAKDGTAERDGRLKVGDKVLSINSRDMKNAKHDDAVNMLTSGPSFVTLIVYRDRVINKKMTPLTRAGKQYNPSPSRAGKQYNPSPARAGKQYSPSPARAEINHEIILKK--GNNPLGFSIVGGSDHASHPFGMDEP-------------GIFISKIVPTGVAATTN--LKIGDRVLMVNGKDMRNATHQDAV 911 HSP 2 Score: 62.003 bits (149), Expect = 7.761e-10 Identity = 65/239 (27.20%), Postives = 97/239 (40.59%), Query Frame = 3
Query: 1347 ESLGMSVAGGVNS---QRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPG--YCQIPRM-----------ITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRP----IMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
+ LG+++AGG S + D ++I+ I+ N + GD +L VN ++ +H +AV+ LK + VR + + T S K G + P M ITL R G LGFSI GG PY+ I I I A ++GRLK GD ++SIN + N H DAVN+ T GP + ++ + +I
Sbjct: 576 KGLGINIAGGKGSTPYKENDEGIFISRISENGPAGRDGILHVGDKILKVNGVDISNATHHQAVDVLKSTGKDITLYVVREKQEIEKRTVPKTAKD-ESVKEEPKKTGVRFAPEPEMEDIETRPEKETITLKRGGDKG-LGFSIAGGKG------------------STPYKDGDPGIFISKIAKDGTAERDGRLKVGDKVLSINSRDMKNAKHDDAVNML--TSGPSFVTLIVYRDRVI 792 HSP 3 Score: 52.373 bits (124), Expect = 7.225e-7 Identity = 83/398 (20.85%), Postives = 149/398 (37.44%), Query Frame = 3
Query: 531 YTPLNHLDNLEFNVEDFTITIINPERLEFGVSFVGG-FDTPLQ----CIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRD-IVDKRWLPV---------------------------------AKEEILTFVLEKPTDRKMGLKLAGKTNKS---------GLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLK-NASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRV 1577
+ P ++++E E TIT+ G S GG TP + I I +I +DG DGRL+ GD++ IN + A H D ++ ++ + L ++RD +++K+ P+ A+ EI ++ K + +G + G ++ + G++I +++P A+ LK DR+L +N D+ T +A+ + + +K ++ P + + S PG K S RG G+ PG L + D ++I+ ++ + + G +L VN LLG +H AV AL R ++VT+ V
Sbjct: 684 FAPEPEMEDIETRPEKETITLKRGGDKGLGFSIAGGKGSTPYKDGDPGIFISKIAKDGTAERDGRLKVGDKVLSINSRDMKNAKH---DDAVNMLTSGPSFVTLIVYRDRVINKKMTPLTRAGKQYNPSPSRAGKQYNPSPARAGKQYSPSPARAEINHEIILKKGNNPLGFSIVGGSDHASHPFGMDEPGIFISKIVPTGVAATTN-LKIGDRVLMVNGKDMRNATHQDAVAALIANVSLIKLLVRH--------------DPPPKGLNDVNFHKS-PGEKLGISIRGGAKGH-------------------------PGNPL----------DKTDEGIFISKVSEGAAAHKDGRLMVGQRILEVNGVSLLGATHLEAVRAL---RSMGDRVTLLV 1024
BLAST of Ligand of Numb protein X 2 vs. Ensembl Nematostella
Match: EDO45887 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRU6]) HSP 1 Score: 60.8474 bits (146), Expect = 1.477e-9 Identity = 112/496 (22.58%), Postives = 195/496 (39.31%), Query Frame = 3
Query: 666 IQEIFRDGRIAMDGRLRP--------GDQITKINEYYLSQASH-IECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVAKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEI---------------------------------------------IKSV-KDSVKFVISRIVRPQ---------TPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIP---SWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPY-RPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFK 1949
+QE + ++ GR +P +Q+ Y+ Q H E + S S D I + +H D E+ L F + KP G ++ GL ++E+ AS +G+L+ DRIL++N +L+ + + A E+ IK+V KD K +S+ PQ +P I R+ G P S +P PG ++ S G P S + K ++E++K G+ LG S+ N G TP+++ +I + GD ++ VN + G + AV L+ + V++L L+ + T I +L+ G + I L+ A G LG S+ G + D +++P I +KS+++G A K+GRLK D L+S+N+ S +++++A++ +
Sbjct: 59 LQEEHQKDQLRSSGRYQPQATVSLPRKEQLNDFPSYHHQQYPHEREHTNSYSSDDSSDDVIMIELHPD----------NEDDLGFTV-KPYKTADGREI-------GLIVREIAAGKCASRNGKLQVADRILEVNGQNLMDLSNSRAQEVFDSAIRSPVIKLAVSASSSFVSGNTKKPTKHDKPPSASFQPEPVVIKAVYKDKEKASMSKPEIPQNFGLPRSPVSPGIPRSPGF-PKSPRSPDGIEPP-PGFRDNSQ-----GAPPPMLSSKVVSKRM----LLELNKG-GDGLGFSITTRDNPAGGHTPIFVKSILAKGAAIEDGRLRGGDQIIEVNGMPMTGKNQGEAVNILR-----STEGVVKLLIQREELVQSPTKPAIELNEDSTRYLREQGKQVLTFKIPLNMSGAAG-LGVSVKG------------KVADTDSGRDSPIDMGIFVKSVIAGGAAFKDGRLKAEDQLLSVNNVSFMRLTNTEAIDGLR 506 HSP 2 Score: 49.2914 bits (116), Expect = 5.483e-6 Identity = 47/202 (23.27%), Postives = 88/202 (43.56%), Query Frame = 3
Query: 633 GGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVL-------------SRISQSGDTIYLTIHRDIVDKRWLPVAKEEILTFV--LEKPTDRKMGLKLAGKTNKS----------GLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVK----FVISRIVRPQTP 1151
G TP I ++ I G DGRLR GDQI ++N ++ + E ++L + QS + ++ D R+L +++LTF L +G+ + GK + G++++ V+ A DGRLK +D++L +N + T EA++ ++ + + F+ + + R + P
Sbjct: 333 AGGHTP---IFVKSILAKGAAIEDGRLRGGDQIIEVNGMPMTGKNQGEAVNILRSTEGVVKLLIQREELVQSPTKPAIELNEDST--RYLREQGKQVLTFKIPLNMSGAAGLGVSVKGKVADTDSGRDSPIDMGIFVKSVIAGGAAFKDGRLKAEDQLLSVNNVSFMRLTNTEAIDGLRRAMQNTRLGQAFIETTVARERDP 529
BLAST of Ligand of Numb protein X 2 vs. Ensembl Medaka
Match: lnx2a (ligand of numb-protein X 2 [Source:NCBI gene;Acc:101160604]) HSP 1 Score: 244.588 bits (623), Expect = 4.448e-70 Identity = 181/564 (32.09%), Postives = 281/564 (49.82%), Query Frame = 3
Query: 456 QDISNTNSGNNFPKPQDCFYQNNSFYTPLNHLDNLEFNVEDFTITIINPER----LEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRD----------------------IVDKRWLPVAKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTP---------DIIRAAGTEPDSNMTPIATSSSYP-GLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKV-------PNLLLMANTD-----NFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
+ +S+T+SG + P+ + P ++ + IT I R +E G+S VGG +TPL +VIQE++RDG IA DGRL GDQI ++N +S H R LSR T+ LT+ R+ P + ++ + ++ + ++G+KL +T++SG+++ ++L A+ DGRL+ +DR+L +N+ DL GT +A +II++ + V +I R +P P D+ P+ + TP S P L ST +S + +E+ + V K+P ESLGM+VAGG S+ G+ P+++T++ P+ L I +GD+LL++N +L LSH AV LK A V +RVL+V + LL D N+ PSW+ WL LP Y I L R + GS GFSIVGG + SH + P IK+IV G+PA+ +GRLKCGD+++++N S + +SHS V + K + V L VVSWPG+++
Sbjct: 238 RSVSSTSSGWDCPESPPLSAEEGCVKLP---------SLPEGEITTIEVHRANPYVELGISVVGGNETPLINVVIQEVYRDGVIARDGRLLAGDQILQVNNVDISNVPHSFARSTLSRPCT---TLQLTVLRERRCASRAPPSSSSSTPAVPHPPCSTPDGTPSSPTKLRITLHKRDSTEQLGIKLVRRTDESGVFVLDLLEGGLAAKDGRLRSNDRLLAVNDQDLRHGTPEQAAQIIQASGERVYLLIGRPSKPTPPPPPKSSSTRDLYCLEHFLPNHSSTP----SPVPIHLSRTSTH------------RDLSQCVSCKEKHITVKKEPHESLGMTVAGGRGSKSGELPIFVTSVQPHGCLSRDGRIKRGDVLLSINSQDLTYLSHSEAVGTLK-ASAASPSVQLRVLEVIMEEEHNHDELLPHTHDSDFDANWSPSWVMWLGLPSYLHSSHEIVLRR-SHPGSWGFSIVGGYEE-------------SHGNQ----PFFIKTIVLGTPAYYDGRLKCGDMIVAVNGLSTAGMSHSALVPMLKEQRSRVALTVVSWPGSLV 754
BLAST of Ligand of Numb protein X 2 vs. Ensembl Medaka
Match: lnx1 (ligand of numb-protein X 1 [Source:NCBI gene;Acc:101156395]) HSP 1 Score: 231.876 bits (590), Expect = 2.672e-65 Identity = 172/547 (31.44%), Postives = 270/547 (49.36%), Query Frame = 3
Query: 516 QNNSFYTPLNHLDNLEFNVEDFTITIINPERLE----FGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKR-----------------------WLPVAKEEILTFVLEK-PTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAA--GTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLK------------VPNLLLMANT---------DNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
+N+S + L L + D +T I R + +S VGG +TPL I+IQ+I+R+G IA DGRL PGD I K+N +S +H C V S + Q + LT+ R+ P +++ + VL K ++G+KL + ++ G++I +L A+ DG+L DR+L IN HDL +G A +I++ + V F++SR + TPDI + A G E +P+ + DS K E+ V + K+P +SLGM+VAGG++S+ D P+Y+TN+ P+ ++ I KGDILL VN +L G++ AV LK + + V ++VL+ +P L + T D++ P W+ WL+LP + + I L R+ +GSLGFSIVGG + + N N S I+SIV G+PA+ +GR++CGDIL+ +N S ++H+ V L K +G + L +VSWPG+++
Sbjct: 279 RNSSVPPEVLALPQLHHLIPDGELTTIKITRSDPSDPLAISIVGGNETPLVRILIQDIYREGVIARDGRLLPGDMILKVNGIDISNVAH--CFAV-SILKQPAQLLRLTVLREQRHHYRSHSQSHPHGHGHEVLSGHQSHGLPPPMRDDSIHVVLNKNAPQEQLGIKLVRRPDEHGVFIFHLLEGGLAARDGQLCIGDRVLAINGHDLRYGAPEHAALLIQASEQRVHFIVSRQICSPTPDIPQEAPWGMEGPPPYSPVDMELTL-------------------LDS--CQKPACYEKTVTLTKEPYDSLGMTVAGGMSSRGWDLPIYVTNVDPDGVVGQEGSIRKGDILLNVNGLDLTGVTRSEAVANLK---NTSSPVVLKVLEMRPPEESLQEFPLPPCLTPSPTDSIKSPVPNDDYSPLWVSWLQLPRHLYYCKDIVLRRN-TSGSLGFSIVGGQEEM---NCNQSF--------------FIRSIVEGTPAYNDGRIRCGDILLEVNGKSTWGLTHTALVRLLKELRGRITLTIVSWPGSLL 780
BLAST of Ligand of Numb protein X 2 vs. Ensembl Medaka
Match: lnx2b (ligand of Numb protein X 2 [Source:NCBI gene;Acc:101170936]) HSP 1 Score: 207.223 bits (526), Expect = 4.143e-57 Identity = 157/510 (30.78%), Postives = 236/510 (46.27%), Query Frame = 3
Query: 600 PERLEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRW-----------LPVAKEEILTFVLEKPT----------DRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLA---------------------RDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
PE+ + G+ VGG +TPL IVIQEI RD A DGRL PGD I ++N+ L+ H VL S T+ + D + P + T + P +G+KL K+ SG+ I ++LP A+ DG+L+ +D++L IN HDL GT A II++ + V FV+ R T D + G + + A Y S + D P F +++E+ V + K+P SLG+++AGG + R PVYIT++ P L I +GDILL++N +L L+++ AV LK ++ NK V + P + N+ P W WL LP + R I L + S GFSIVGG + SH ++ P+ IK+IV G+PAH +GRLKCGD ++++N + + ++ + + K + V L VVSWPGT++
Sbjct: 222 PEQ-DLGLRIVGGKNTPLGNIVIQEIIRDSIAACDGRLAPGDHILEVNDVSLASVPHSRAIAVLRHPSLVRLTVMQEKGFKLRDSQRSDPHPSPSTASQPPRSPQCNTHFNQTPVLHVTLVKSHRSEPLGIKLIRKSEFSGVVILDLLPGGLAAKDGKLRNNDKVLAINGHDLRHGTPESAAHIIQTSEMRVNFVVMR-----TADTVEDGGN---------GREGQHARARRAPDPQYFRRQSTYIKDPPSGF--SFQEKNVSLKKEPRHSLGITIAGGRDC-RSRLPVYITSVQPVGCLHREGYIKRGDILLSINGVDLTQLTYKEAVSVLKAETAQSQVELCVIQTHSEDPEEDSESSNKDEVVYTEDPR----EDNRNWTPQWTRWLGLPSHMHWCRDIVLQK-TNNESWGFSIVGGYEE-------------SHGQQQPF---FIKTIVPGTPAHFDGRLKCGDEIVAVNGATTVGMKNNSLIPMLKLQKNRVTLTVVSWPGTLV 692
BLAST of Ligand of Numb protein X 2 vs. Ensembl Medaka
Match: MPDZ (multiple PDZ domain crumbs cell polarity complex component [Source:NCBI gene;Acc:101165593]) HSP 1 Score: 172.555 bits (436), Expect = 3.964e-44 Identity = 143/470 (30.43%), Postives = 228/470 (48.51%), Query Frame = 3
Query: 615 FGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVAKEEIL----TFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYP-----GLKNASTRGYEVGNSPFTFDSPISFKSTYRE-EVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMA-NTDNFIPSWMYW--LKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVS 1985
G+S VGG DT L I+I E++ +G + DGRL GDQI ++N L ASH + +VL + Q + LT+ RD + ++ KEE L L K + +GL + G+ N +G+++ +++ DGRL + D+IL +N D+ TQ ++K +K + R AG P + ++ SS P L S G P + DS + +++ VE+ K P +SLG+S+AGGV S GD P++I + P L T + GD ++++ T G+SH +AV LK + + ++V+ + + + + PS ++ + P Y + ITL R LGFSIVGG+ S DL PI +K++ A ++GRLK GD +I++N +L V+H +AV + K T+G V L V+S
Sbjct: 1274 LGLSIVGGCDTLLGAIIIHEVYEEGAASKDGRLWAGDQILEVNGIDLRAASHDDAINVLRQTPQR---VRLTVFRD--EGQY----KEEDLWDSFDVELHKKPGQGLGLSIVGRRNDTGVFVSDIVSGGLVDADGRLLQGDQILSVNGEDVRSATQEAVAALLKCCIGPIKLEVGRF----------KAG--PFHSERRLSQSSQVPCVEILTLLQISESGSSKVARPSSSDSGNLLSAEHQDVRTVELTKGPSDSLGISIAGGVGSPLGDIPIFIAMMNPVGLAAQTQ-LKVGDRIVSICGTSAEGMSHSQAVTLLK---NTPGTIQLQVVAGGDSTVTGPSQEQLTPSCIFQDDIGPPQY----KTITLER--GPDGLGFSIVGGH--------GSPHGDL---------PIYVKTVFGKGAAAEDGRLKRGDQIIAVNGQTLEGVTHEEAVGILKRTKGTVTLTVLS 1695 HSP 2 Score: 90.1225 bits (222), Expect = 4.979e-18 Identity = 102/410 (24.88%), Postives = 168/410 (40.98%), Query Frame = 3
Query: 915 RKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIV-RPQTPDIIRAAGTEP--DSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPI-------SFKSTYRE----------EVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHER-------AVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFI--------PSW---MYWLKLPGYCQIPRMITLSRDAAT-----------------GSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKV 1979
R MG +L+ G++I+ +L DS A +G L+ DRI++++ DL + EA+E I+ + V F++ IV RP+ + + +P S ++P T + P + +A + F T+ + ++E++K LG+S+AG N R V++ I PN I GD LL +N L G SH+ A+ +K+ + C K ++L V + ++ T + + P+ +WL G+ + TL+ D AT LG SIVGG D + I+I + A K+GRL GD ++ +N L SH DA+N+ + T V L V
Sbjct: 963 RGMGSRLSNGEVMRGIFIKHILEDSPAGQNGTLRTGDRIVEVDGVDLRDASHEEAVEAIRRGGNPVTFLVQSIVQRPRVSQLKQ----QPFGLSEISPTGTLLALPVVPHAGESDTDALAEAPAEAGEEGEEEKVDEFGYTWSDRRYGSLPGVLHMIELEKGQ-TGLGLSLAG--NRDRSRMSVFVVGIDPNGAAGRDGRILVGDELLEINGQVLYGQSHQNASSIIKSALSKVKIIFEGCMKPGDKLLAVNGVSVLGYTVDKVLNDESLVDPTSSCPFFWL---GFSRSSTPSTLACDPATCPIIPGCETTIEICKGCTGLGLSIVGGCDTLLG-------------------AIIIHEVYEEGAASKDGRLWAGDQILEVNGIDLRAASHDDAINVLRQTPQRVRLTV 1343 HSP 3 Score: 85.5001 bits (210), Expect = 1.123e-16 Identity = 121/510 (23.73%), Postives = 201/510 (39.41%), Query Frame = 3
Query: 582 TITIINPERLEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKR-------WLPVA----------KEEILTF-VLEKPTDRKMGLKLAG-----KTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALK----LARDNC---------------NKVTVRVLKVPNLLLMAN-TDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQ-GPVHLKV 1979
TI ++N + G VGG T ++++ I G DGRLR GD I +I + L + VL Q G+ + L + R V++ LP +EE +F V + +G+ +AG + SG++++ + DS DGR+ D+I ++ ++ T +A+E+++ +V+ + R R P+ I A + S ++P S++ P E +V +K ES G+ ++ + + RG YI ++ P + + GD L+ VN L+G +H+ V LK C +VT P T+ I S + +L + + + G LGFSI+ DP+ P T I+I+S+V A +GRL GD L+ +ND +L N S DAV K + G V + V
Sbjct: 247 TIELVN-DGTGLGFGIVGGKTT---GVIVKTILPGGIADQDGRLRSGDHILRIGDTDLHGMGSEQVAQVLR---QCGNRVKLVVTRAPVEETPSAVMSVVLPTVSEQQVPKQNKEEEAESFNVCLTKNAQGLGITIAGYVGDKNSEPSGIFVKSITKDSAVDQDGRIHVGDQITAVDGVNIQGHTNQQAVELLRHTGQTVRLKLIR--RGFRPEEIPPAVSPSASVLSP---SAAIPTTTTVMRELELERRQEEVIKKWQKILGPSNEVIVAQVEKFSESSGLGIS--LEASRGHH--YIRSVLPEGPVGRCGQLFSGDELVEVNGISLIGETHKEVVRILKELPIFVYMTCCRPPETQSERSDPRSEQVTEESAGNPEAATQDTVTEEGIGSSLAMWEL-------EIQNIELEKGEGGLGFSILDYQDPLDPAKT----------------VIVIRSLVPDGVAETDGRLLPGDRLMYVNDTNLENASLEDAVQALKGAKLGKVQIGV 717 HSP 4 Score: 83.9593 bits (206), Expect = 4.227e-16 Identity = 92/380 (24.21%), Postives = 160/380 (42.11%), Query Frame = 3
Query: 897 LEKPTDRKMGLKLAGKTNKS----GLYIQEVLPDSQASLDGRLKKDDRILKINEHDL-VFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYRE-EVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYC--QIPRM----------ITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVV 1982
L+KP +G L G +++ G++IQE+ P S A DG+LK+ D+IL IN L + +A+ +++S + V ++R PQ T P + TP A S+ +S S+++ E +E+ G LG + GG T V + I P + + GD +L + +T+L G+ E+ + L R N+V + V + P PS + + LP Q+P+ + L+++A LG +I G Y + NS + I +KSI S ++GR+ GD + +++ ++ ++ AV L + T V LK++
Sbjct: 133 LQKPASGGLGFSLVGLKSENRGELGIFIQEIQPGSVADCDGKLKEADQILAINGQPLDQTVSHQQAIGLLQSASERVHLTVARGPIPQL--------TSPIVSRTPSAAST-------------------------LSANSSWQHVETIELVND-GTGLGFGIVGGKT-----TGVIVKTILPGGIADQDGRLRSGDHILRIGDTDLHGMGSEQVAQVL---RQCGNRVKLVVTRAPV--------EETPSAVMSVVLPTVSEQQVPKQNKEEEAESFNVCLTKNAQ--GLGITIAG-----YVGDKNSEPSG-----------IFVKSITKDSAVDQDGRIHVGDQITAVDGVNIQGHTNQQAVELLRHTGQTVRLKLI 444 HSP 5 Score: 70.0922 bits (170), Expect = 6.423e-12 Identity = 107/458 (23.36%), Postives = 186/458 (40.61%), Query Frame = 3
Query: 660 IVIQEIFRDGRIA-MDGRLRPGDQITKINEYYLSQ-ASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVAK---------------EEILTF-VLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGD-TPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIY-PFNTN--------SSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFK 1949
I IQEI + G +A DG+L+ DQI IN L Q SH + +L S+ ++LT+ R + + P+ + + T ++ T G+ + GKT +G+ ++ +LP A DGRL+ D IL+I + DL + ++++ + VK V++R +TP + S + P + P K E N T K + LG+++AG V + + + +++ +I +S + I GD + AV+ + G ++++AVE L+ +R P + A + PS L IP T+ R+ ++ I P N S + L + EA I+S++ P + G+L GD L+ +N SL +H + V + K
Sbjct: 158 IFIQEI-QPGSVADCDGKLKEADQILAINGQPLDQTVSHQQAIGLLQSASER---VHLTVARGPIPQLTSPIVSRTPSAASTLSANSSWQHVETIELVNDGTGLGFGI-VGGKT--TGVIVKTILPGGIADQDGRLRSGDHILRIGDTDLHGMGSEQVAQVLRQCGNRVKLVVTRAPVEETPSAVM-------SVVLPTVSEQQVP--KQNKEEEAESFNVCLT--------------------KNAQGLGITIAGYVGDKNSEPSGIFVKSITKDSAVDQDGRIHVGDQITAVDGVNIQGHTNQQAVELLRHTGQTVRLKLIRRGFRPEEIPPAVS----PSASV---LSPSAAIPTTTTVMRELELERRQEEVIKKWQKILGPSNEVIVAQVEKFSESSGLGISLEASRGHHYIRSVLPEGPVGRCGQLFSGDELVEVNGISLIGETHKEVVRILK 572 HSP 6 Score: 62.3882 bits (150), Expect = 1.509e-9 Identity = 98/393 (24.94%), Postives = 154/393 (39.19%), Query Frame = 3
Query: 951 KSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVK-------------DSVKFVISRIVRPQTPDIIRA-------AGTEPDS---NMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKL----------ARDN-CNKVTVRVLKVP-NLLLMANTDNFIPSWMYWLKLP-------------GYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVS 1985
K+ + I+ ++PD A DGRL DR++ +N+ +L + +A++ +K K S+ I + P D+ +G E D+ +M + S+ L + +VG F F SF YR++ D+ G + V++ + + I +I + + GD++LA+N LS+ +A L+ R N C V + VP L PS LP G PR + L R+ SLG SIVGG S L+ N E R I IK I+ SPA +NG L+ GD ++ ++ L + SH +AV + PV V S
Sbjct: 659 KTVIVIRSLVPDGVAETDGRLLPGDRLMYVNDTNLENASLEDAVQALKGAKLGKVQIGVAKPLPVSLSVSIPTFIDPCEGDLSDERMLDHTYSGVEEDTFQVSMIALHGSACSTDLDYLQSSTPKVGTC-FGFGG--SFFDVYRQKYE--DQFNGCIMSYPKGMTVSAMKDGLGMLIRSIIHGGSISRDGRLGVGDLILAINGEPTTNLSNAQARAMLRRHSLIGPDLGQRRSNFCVFVGFSITYVPAEYLEEYKLQCLPPSSRMISTLPEREDGEGEESASYGNWNQPRKVELFREHGK-SLGISIVGGR------GMGSRLS----NGEV-MRGIFIKHILEDSPAGQNGTLRTGDRIVEVDGVDLRDASHEEAVEAIRRGGNPVTFLVQS 1034 HSP 7 Score: 60.8474 bits (146), Expect = 4.562e-9 Identity = 57/224 (25.45%), Postives = 92/224 (41.07%), Query Frame = 3
Query: 1323 VEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETEL-LGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWP 1991
VE+ K LG S+ G + RG+ ++I I P S+ + + D +LA+N L +SH++A+ L+ A + +V + V + P L + + PS L Q I L D LGF IVGG +++K+I+ G A ++GRL+ GD ++ I D L + + + V L V P
Sbjct: 131 VELQKPASGGLGFSLVGLKSENRGELGIFIQEIQPGSVADCDGKLKEADQILAINGQPLDQTVSHQQAIGLLQSASE---RVHLTVARGPIPQLTSPIVSRTPSAASTLSANSSWQHVETIELVNDGT--GLGFGIVGGK----------------------TTGVIVKTILPGGIADQDGRLRSGDHILRIGDTDLHGMGSEQVAQVLRQCGNRVKLVVTRAP 327 HSP 8 Score: 55.0694 bits (131), Expect = 2.570e-7 Identity = 88/406 (21.67%), Postives = 167/406 (41.13%), Query Frame = 3
Query: 555 NLEFNVEDFTITII-NPERLEFGVS-FVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHR-----------------------------------------DIVDKRWLPVA--KEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQR----GDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFI 1625
N E E F + + N + L ++ +VG ++ I ++ I +D + DGR+ GDQIT ++ + ++ + ++L +G T+ L + R + V K+W + E++ +EK ++ GL ++ + ++ YI+ VLP+ G+L D ++++N L+ T E + I+K + FV RP R ++P S ++ + + +G+S ++ I + +E++K G LG S+ ++ Q T + I ++ P+ + + + GD L+ VN+T L S E AV+ALK A+ KV + V K + L + FI
Sbjct: 352 NKEEEAESFNVCLTKNAQGLGITIAGYVGDKNSEPSGIFVKSITKDSAVDQDGRIHVGDQITAVDGVNIQGHTNQQAVELLR---HTGQTVRLKLIRRGFRPEEIPPAVSPSASVLSPSAAIPTTTTVMRELELERRQEEVIKKWQKILGPSNEVIVAQVEKFSESS-GLGISLEASRGHHYIRSVLPEGPVGRCGQLFSGDELVEVNGISLIGETHKEVVRILKELP---IFVYMTCCRPPETQSER---SDPRSEQVTEESAGNPEAATQDTVTEEGIGSSLAMWELEI--------QNIELEKGEG-GLGFSI---LDYQDPLDPAKTVIVIRSLVPDGVAETDGRLLPGDRLMYVNDTNLENASLEDAVQALKGAK--LGKVQIGVAKPLPVSLSVSIPTFI 733
BLAST of Ligand of Numb protein X 2 vs. Ensembl Medaka
Match: MPDZ (multiple PDZ domain protein-like [Source:NCBI gene;Acc:101166191]) HSP 1 Score: 167.162 bits (422), Expect = 2.258e-42 Identity = 141/478 (29.50%), Postives = 224/478 (46.86%), Query Frame = 3
Query: 615 FGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKR----WLPVAKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRI-----VRPQTPDIIRAAGTEPD----SNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEV--VEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVL------KVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVS 1985
G+S VGG DT L ++I E+ G DGRL+ GDQI ++N L QA+H E VL +Q + L I R R W ++ + L +G GK+N +G+++ +V+ A DGRL D+IL IN D+ +Q+ A ++++S +V ++R Q+ D + + + S +TP + S + S+RG +S S+ EV V V K P +SLG+S+AGGV S G+ P++I + N L T + GD +L +N+ G++H+ EA L ++ VT++V + + + P + W + + ITL R + LGFSIVGG S DL PI +K+I + A ++GRLK GD +I++N L V+H++AV++ K T+G V L ++S
Sbjct: 1481 LGLSIVGGCDTLLGAVIIHEVNDGGAAQRDGRLQAGDQILEVNSIDLRQATHDEAIAVLRLTTQQ---VRLCIFRHQEAYREEDLW------DVFSLKLSPRPGEGLGFTTVGKSNDTGIFVSDVIRGGAADSDGRLLPGDQILSINGVDVRAASQDHAQKLLQSSSGAVLLEVARFKARVQYSRQSQDYAKFCFIQHEDSDCSTLTPTSGS-------DTSSRGA---------NSKAKCSSSQDREVRKVTVQKGPRDSLGISIAGGVGSPHGNVPLFIATMDTNGLAAKTEKLQTGDQILCINDVSTEGMTHD---EAGALLKNAAGSVTLQVTADSGGGSRQDHSAVHAASSSQPGSLTWFHNNLCPRKYKTITLER--GSSGLGFSIVGG--------FGSPHGDL---------PIYVKTIFNKGAAVEDGRLKRGDQIIAVNGHCLEGVTHAEAVDILKRTKGTVVLTLLS 1911 HSP 2 Score: 65.855 bits (159), Expect = 1.315e-10 Identity = 88/391 (22.51%), Postives = 149/391 (38.11%), Query Frame = 3
Query: 915 RKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVI-SRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEV--------------------------------VEVDKKPG-ESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWM-YWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVV 1982
R MG +L+ G++I+ + PDS A+ +G L+ DRIL+++ DL + EA+E I+ DSV ++ S R Q+P T P SN + L + + +PF SP S + R V +E++K P LG+++AG + R V + ++ P I GD LL +N L G S++ A + A V +R + + W C + I L++D A LG +V DP + +++ S++ A K+GR+K GD ++ + ++ +S +L Q V L ++
Sbjct: 1035 RGMGRRLSSGEMMRGVFIKHISPDSPAAHNGTLRAGDRILEVSGVDLRDASHEEAVEAIRRAGDSVVLLVRSGQDRCQSPAPTCRTPT-PQSNSHSSKEEEALSSLFLSVSHRNPFTPTPFKAPSPTSDRMDGRIPVNATAAAAAEDGDDACRKKMLQRYGSLSGKLHMIELEKDPAVPGLGITLAGSKDGSRARLSVCVASVDPQGPTGLDGRIQVGDELLEINGKILYGRSYQNASTIISNAPSKVKIVLIRNKAGKAVKENRTEGEVVIDWHPDAADEEESCSDLKRIVLTQDHA--GLGIRLV--EDP-------------------SWEGVVVGSLIQDGSASKDGRIKVGDRILEVGGEPVAGLSVEKVSSLISKQQVAVKLSII 1401 HSP 3 Score: 61.6178 bits (148), Expect = 2.911e-9 Identity = 107/491 (21.79%), Postives = 188/491 (38.29%), Query Frame = 3
Query: 615 FGVSFVGGFDTPLQ--CIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKR-------------WLPVAKEEI-----LTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGN-----SPFTFDSPISFKSTY-REEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPS--W-MYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTI 2000
G++ G D + + + G +DGR++ GD++ +IN L S+ + IS + + + + R+ K W P A +E L ++ +G++L + G+ + ++ D AS DGR+K DRIL++ + + + +I + +VK I R P + + P + P+ + S L T E N SP + D P+S RE +E+ K LG+S+ GG ++ G V I + + + GD +L VN +L +H+ A+ L+L L + + + + W ++ LKL PR LGF+ VG ++ I + ++ G A +GRL GD ++SIN + S A L +S+ G V L+V + +
Sbjct: 1214 LGITLAGSKDGSRARLSVCVASVDPQGPTGLDGRIQVGDELLEINGKILYGRSYQNASTI---ISNAPSKVKIVLIRNKAGKAVKENRTEGEVVIDWHPDAADEEESCSDLKRIVLTQDHAGLGIRLVEDPSWEGVVVGSLIQDGSASKDGRIKVGDRILEVGGEPVAGLSVEKVSSLISKQQVAVKLSIIRSKSPLSSPLFPPPSPLPHPSCIPLPSPPSPLTLSTPPTPPSEHSNLLTCSSPSSCD-PVSCPVVAGRETTIEIHKG-NVGLGLSIVGGCDTLLG--AVIIHEVNDGGAAQRDGRLQAGDQILEVNSIDLRQATHDEAIAVLRLTTQQV-----------RLCIFRHQEAYREEDLWDVFSLKLS-----PR--------PGEGLGFTTVGKSNDTG---------------------IFVSDVIRGGAADSDGRLLPGDQILSINGVDVRAASQDHAQKLLQSSSGAVLLEVARFKARV 1652 HSP 4 Score: 55.4546 bits (132), Expect = 2.007e-7 Identity = 60/224 (26.79%), Postives = 103/224 (45.98%), Query Frame = 3
Query: 1317 EVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANT--DNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVV 1982
E++E+ G+ LG + GG S + + I P + GD +L + +T+L G++ E+A + L R+ +KV LL+ N DN + S + L ++I L+ D SL F+ N F +S + DL+ A +M+KSI GS ++GR+ GDI++S++ SL S A+ + + T V L+++
Sbjct: 165 EIIELTND-GKGLGFGIIGGRASG-----IMVKTILPGGAAGRDTRLRSGDQILRIGDTDLAGMNSEQAAQVL---RNAGSKV--------KLLIARNVTKDNHLSSPV----LSQDSSDDKIIDLNDDYEF-SLQFT---KNSRGLGFTVSSYVGDLNSVYSAG---VMVKSIAKGSTVDQDGRMHIGDIILSVDGVSLQGCSEQRAMEVLRRTGPLVRLRLL 360 HSP 5 Score: 52.373 bits (124), Expect = 1.859e-6 Identity = 65/242 (26.86%), Postives = 106/242 (43.80%), Query Frame = 3
Query: 852 KRWLPVA--KEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFD------SPISFKSTYRE---EVVEVDKKPGE-SLGMSVAGGVNSQRG-DTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALK 1538
KRW + E+ L++ ++ GL ++ + Y+ +LP+ G++ DRIL++N LV T E + I+K + SV V SR+V P P+ E D N ++ + +G + P D P+S E +VVE++K GE LG S+ + + T + I ++ P + + GD L+ VNET+L G S + AV LK
Sbjct: 442 KRWQHAVGPRYEVTVCQLQRFSETS-GLGISLEARAGHHYLCSILPEGPVGQSGKILIGDRILEVNGIPLVGETHKEVVNILKELPRSVHLVCSRVVPPSIPE-----SEEDDDNDVQLSLKELLAEFNDKLDQGCVI-PCPTAEDITKRGVPPMSPPLAMWEKDAQVVELEK--GELGLGFSILDYQDPEDATKTVLIIRSLVPGGVADRDGRLLPGDRLMFVNETDLEGSSLDYAVHVLK 674
BLAST of Ligand of Numb protein X 2 vs. Planmine SMEST
Match: SMESG000067101.1 (SMESG000067101.1) HSP 1 Score: 1269.6 bits (3284), Expect = 0.000e+0 Identity = 628/628 (100.00%), Postives = 628/628 (100.00%), Query Frame = 3
Query: 120 MSIDPKIKSKFSSFKNDDRLMSVTVTSSPCQEFIPKSEIDPNLIKQCQGLFVSCCYSTAGCTFKAVGREVAAHEWQCPYGQKSPEXXXXXXXXXXXXXVFQTDAAGVSINGSQDISNTNSGNNFPKPQDCFYQNNSFYTPLNHLDNLEFNVEDFTITIINPERLEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVAKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
MSIDPKIKSKFSSFKNDDRLMSVTVTSSPCQEFIPKSEIDPNLIKQCQGLFVSCCYSTAGCTFKAVGREVAAHEWQCPYGQKSPESSLTSITLSSTSSVFQTDAAGVSINGSQDISNTNSGNNFPKPQDCFYQNNSFYTPLNHLDNLEFNVEDFTITIINPERLEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVAKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII
Sbjct: 1 MSIDPKIKSKFSSFKNDDRLMSVTVTSSPCQEFIPKSEIDPNLIKQCQGLFVSCCYSTAGCTFKAVGREVAAHEWQCPYGQKSPESSLTSITLSSTSSVFQTDAAGVSINGSQDISNTNSGNNFPKPQDCFYQNNSFYTPLNHLDNLEFNVEDFTITIINPERLEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVAKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 628
BLAST of Ligand of Numb protein X 2 vs. Planmine SMEST
Match: SMESG000010833.1 (SMESG000010833.1) HSP 1 Score: 452.981 bits (1164), Expect = 4.497e-147 Identity = 282/637 (44.27%), Postives = 394/637 (61.85%), Query Frame = 3
Query: 171 DRLMSVTVTSSPCQEFIPKSEIDPNLIKQCQGLFVSCCYSTAGCTFKAVGREVAAHEWQCPYGQKSPEXXXXXXXXXXXXXVFQTDAAGVS---------INGS---QDIS-NTNSGNNFPKPQDCFYQNNSFYTPL------NHLDNLEFNVEDFTITIINPERL---EFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVAKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYP----FNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
D+L+ + C E + +SE++ +L+ C+ + S AG F +W C + +KSP ++ T++T+SST+S D +G S I+ S Q+++ TN+ + ++N + + N+ + E N+ + + II +R + G+SFVGG DTPLQCIVIQEI+ DG +A DGRLRPGDQI IN+ L+QA+H++ R + Q L ++R+ D+ KEEIL F L K TD+K+G+KL G+ NK GLY+ +++ S+A LDGRL K+DRIL IN DL TQ+ A +I S ++ V ++SRI+RPQTPD+IR G E + T +++ NA+ + +S E+VV V+KK GESLGMSVAGGVNSQRGDTPVY+TN+A + +L T + KGDILLAVN+TELLGLSHE+AVEALK A C V+VR+L+ P NF+PSW+YWL+LP YC+IPR ITL RD+ATGSLGFSIVGG D + + N+++T S N ++ I++KSIV+GS AH+NGRLKCGDIL+ +N+ SL NVSH DAV++FK+ Q V L+VVSWPGTI+
Sbjct: 309 DKLLINCPNNDGCDEVMARSELEAHLLYWCRAIPQSNNNLNAGPMFLVSKSSSGNMKWDCGF-EKSPNTTSTNVTISSTNSGPINDPSGSSTAVNTCEEPISASSSPQEVNVKTNANRILSNINNNHHKNTVRFDAVPHSRKKNYSASEEHNICNDCLQIIVIDRSTNSDMGISFVGGSDTPLQCIVIQEIYLDGAVAKDGRLRPGDQIISINDVDLNQATHLQARQAFLQAQQQPGVNRLMVYRERADESTF-TEKEEILQFGLSKRTDKKLGIKLVGQKNKPGLYVLDLVKGSEADLDGRLHKNDRILNINGVDLRMETQDTAARVINSAEEEVIIIVSRIIRPQTPDLIRNTGPEFAESFTSTCIVNNFSQPPNANKK--------------LSCTGRLFEKVVTVEKKYGESLGMSVAGGVNSQRGDTPVYVTNLAVDGVLAKTQLLAKGDILLAVNDTELLGLSHEKAVEALKSAGQKCKNVSVRLLQGPETYKNGG-HNFLPSWVYWLQLPKYCEIPRAITLKRDSATGSLGFSIVGGVDTVQTSPSRYLLNNNVTGRS-NANTFHQSIVVKSIVTGSVAHRNGRLKCGDILLKVNNTSLINVSHDDAVSIFKNVQDEVVLQVVSWPGTIV 927
BLAST of Ligand of Numb protein X 2 vs. Planmine SMEST
Match: SMESG000010833.1 (SMESG000010833.1) HSP 1 Score: 452.595 bits (1163), Expect = 6.323e-147 Identity = 282/637 (44.27%), Postives = 394/637 (61.85%), Query Frame = 3
Query: 171 DRLMSVTVTSSPCQEFIPKSEIDPNLIKQCQGLFVSCCYSTAGCTFKAVGREVAAHEWQCPYGQKSPEXXXXXXXXXXXXXVFQTDAAGVS---------INGS---QDIS-NTNSGNNFPKPQDCFYQNNSFYTPL------NHLDNLEFNVEDFTITIINPERL---EFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVAKEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDIIRAAGTEPDSNMTPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTYREEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYP----FNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
D+L+ + C E + +SE++ +L+ C+ + S AG F +W C + +KSP ++ T++T+SST+S D +G S I+ S Q+++ TN+ + ++N + + N+ + E N+ + + II +R + G+SFVGG DTPLQCIVIQEI+ DG +A DGRLRPGDQI IN+ L+QA+H++ R + Q L ++R+ D+ KEEIL F L K TD+K+G+KL G+ NK GLY+ +++ S+A LDGRL K+DRIL IN DL TQ+ A +I S ++ V ++SRI+RPQTPD+IR G E + T +++ NA+ + +S E+VV V+KK GESLGMSVAGGVNSQRGDTPVY+TN+A + +L T + KGDILLAVN+TELLGLSHE+AVEALK A C V+VR+L+ P NF+PSW+YWL+LP YC+IPR ITL RD+ATGSLGFSIVGG D + + N+++T S N ++ I++KSIV+GS AH+NGRLKCGDIL+ +N+ SL NVSH DAV++FK+ Q V L+VVSWPGTI+
Sbjct: 322 DKLLINCPNNDGCDEVMARSELEAHLLYWCRAIPQSNNNLNAGPMFLVSKSSSGNMKWDCGF-EKSPNTTSTNVTISSTNSGPINDPSGSSTAVNTCEEPISASSSPQEVNVKTNANRILSNINNNHHKNTVRFDAVPHSRKKNYSASEEHNICNDCLQIIVIDRSTNSDMGISFVGGSDTPLQCIVIQEIYLDGAVAKDGRLRPGDQIISINDVDLNQATHLQARQAFLQAQQQPGVNRLMVYRERADESTF-TEKEEILQFGLSKRTDKKLGIKLVGQKNKPGLYVLDLVKGSEADLDGRLHKNDRILNINGVDLRMETQDTAARVINSAEEEVIIIVSRIIRPQTPDLIRNTGPEFAESFTSTCIVNNFSQPPNANKK--------------LSCTGRLFEKVVTVEKKYGESLGMSVAGGVNSQRGDTPVYVTNLAVDGVLAKTQLLAKGDILLAVNDTELLGLSHEKAVEALKSAGQKCKNVSVRLLQGPETYKNGG-HNFLPSWVYWLQLPKYCEIPRAITLKRDSATGSLGFSIVGGVDTVQTSPSRYLLNNNVTGRS-NANTFHQSIVVKSIVTGSVAHRNGRLKCGDILLKVNNTSLINVSHDDAVSIFKNVQDEVVLQVVSWPGTIV 940
BLAST of Ligand of Numb protein X 2 vs. Planmine SMEST
Match: SMESG000061485.1 (SMESG000061485.1) HSP 1 Score: 350.517 bits (898), Expect = 3.502e-109 Identity = 223/659 (33.84%), Postives = 334/659 (50.68%), Query Frame = 3
Query: 171 DRLMSVTVTSSPCQEFIPKSEIDPNLIKQCQGLFVSCCYSTAGCTFKAVGREVAAHEWQCPYGQKSPEXXXXXXXXXXXXXVFQTDAAGVSINGSQDISNTNSGNNFPKPQDCFYQNNSFYTPLNHLDNLEFNVEDFTITIINPERLEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVA-KEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDII---------------------------RAAGTEPDSNM-----------TPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTY---------REEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
D+L V C+E + ++++ +L +C+G + C ++ GCTF+ + H+W+C Y + ++ + SN G + DC + + +D E I II + + G SF+GG D+PL CIVIQEI+ DG I+ D RLRPGDQI ++N + L+ A+H+E + LS +S R ++ + PV+ K EI T L K D ++G+ L K N G+YI+ ++P S+A ++G L K DRIL IN ++ G+Q A +IIK ++V V+ R R PDI+ R + P S M + I + + P + N+ + V D+ + + ++Y +++ + V KKP ESL ++V GG+NS RGD P+YITNI P L + KG ILL +N LL L+H+ AV+ALK N + V + +L+ P + NF+PSW YWL+LP +C P++I L +D SLGFSIVGG D NTN +L+ IMIK+I+ GS A NG+LKCGDIL+ +N+ SLS++SH+DAV K G VHLK++SWPGTI+
Sbjct: 207 DKLKVVCPNVDYCEEILYRNDLYHHLKYKCRGAVMPCINASIGCTFQGSRNTFSTHQWECKYKD------------------VENGRDPIANDVYNFKSNLEDGTENNEDIDCNSRKETVIDKAQIIDG-----EPCEIEIIRVNQCDLGFSFIGGIDSPLGCIVIQEIYLDGIISQDCRLRPGDQILEVNNFDLTNATHVEAKRALSMLSSKC--------RFVIFRECAPVSNKLEIFTVTLVKRHDSQLGIVLDKKRNHEGIYIRNIIPGSEAYMNGDLAKGDRILSINNIEIGNGSQEYAAKIIKEALENVILVVQR--RLSVPDIVKTTDDRGSLGSWSTSSTGGGGGGGGMSRDSKAPPQSEMISRRQTISSNKSSIWSDHNSPRINNSLNQPRWVPIDQAPVDNWMHYDNSYQHLFVSCTLKQDTINVHKKPNESLSITVTGGINSHRGDIPIYITNIYPTGALSAHRKVKKGSILLRINNINLLHLTHKHAVDALKQECYNSSLVQLHILEGPE--SSSGAGNFMPSWKYWLQLPFFCAFPKLIELYKDNTNISLGFSIVGGIDS--ANNTNHALSFWD-------TCIMIKTILPGSIAFINGKLKCGDILLEVNNVSLSHISHADAVLCLKKVTGRVHLKIISWPGTIV 821
BLAST of Ligand of Numb protein X 2 vs. Planmine SMEST
Match: SMESG000061485.1 (SMESG000061485.1) HSP 1 Score: 350.132 bits (897), Expect = 4.341e-109 Identity = 223/659 (33.84%), Postives = 334/659 (50.68%), Query Frame = 3
Query: 171 DRLMSVTVTSSPCQEFIPKSEIDPNLIKQCQGLFVSCCYSTAGCTFKAVGREVAAHEWQCPYGQKSPEXXXXXXXXXXXXXVFQTDAAGVSINGSQDISNTNSGNNFPKPQDCFYQNNSFYTPLNHLDNLEFNVEDFTITIINPERLEFGVSFVGGFDTPLQCIVIQEIFRDGRIAMDGRLRPGDQITKINEYYLSQASHIECRDVLSRISQSGDTIYLTIHRDIVDKRWLPVA-KEEILTFVLEKPTDRKMGLKLAGKTNKSGLYIQEVLPDSQASLDGRLKKDDRILKINEHDLVFGTQNEALEIIKSVKDSVKFVISRIVRPQTPDII---------------------------RAAGTEPDSNM-----------TPIATSSSYPGLKNASTRGYEVGNSPFTFDSPISFKSTY---------REEVVEVDKKPGESLGMSVAGGVNSQRGDTPVYITNIAPNSLLKNTNGIDKGDILLAVNETELLGLSHERAVEALKLARDNCNKVTVRVLKVPNLLLMANTDNFIPSWMYWLKLPGYCQIPRMITLSRDAATGSLGFSIVGGNDPIYPFNTNSSLTDLSHNKEAPYRPIMIKSIVSGSPAHKNGRLKCGDILISINDFSLSNVSHSDAVNLFKSTQGPVHLKVVSWPGTII 2003
D+L V C+E + ++++ +L +C+G + C ++ GCTF+ + H+W+C Y + ++ + SN G + DC + + +D E I II + + G SF+GG D+PL CIVIQEI+ DG I+ D RLRPGDQI ++N + L+ A+H+E + LS +S R ++ + PV+ K EI T L K D ++G+ L K N G+YI+ ++P S+A ++G L K DRIL IN ++ G+Q A +IIK ++V V+ R R PDI+ R + P S M + I + + P + N+ + V D+ + + ++Y +++ + V KKP ESL ++V GG+NS RGD P+YITNI P L + KG ILL +N LL L+H+ AV+ALK N + V + +L+ P + NF+PSW YWL+LP +C P++I L +D SLGFSIVGG D NTN +L+ IMIK+I+ GS A NG+LKCGDIL+ +N+ SLS++SH+DAV K G VHLK++SWPGTI+
Sbjct: 204 DKLKVVCPNVDYCEEILYRNDLYHHLKYKCRGAVMPCINASIGCTFQGSRNTFSTHQWECKYKD------------------VENGRDPIANDVYNFKSNLEDGTENNEDIDCNSRKETVIDKAQIIDG-----EPCEIEIIRVNQCDLGFSFIGGIDSPLGCIVIQEIYLDGIISQDCRLRPGDQILEVNNFDLTNATHVEAKRALSMLSSKC--------RFVIFRECAPVSNKLEIFTVTLVKRHDSQLGIVLDKKRNHEGIYIRNIIPGSEAYMNGDLAKGDRILSINNIEIGNGSQEYAAKIIKEALENVILVVQR--RLSVPDIVKTTDDRGSLGSWSTSSTGGGGGGGGMSRDSKAPPQSEMISRRQTISSNKSSIWSDHNSPRINNSLNQPRWVPIDQAPVDNWMHYDNSYQHLFVSCTLKQDTINVHKKPNESLSITVTGGINSHRGDIPIYITNIYPTGALSAHRKVKKGSILLRINNINLLHLTHKHAVDALKQECYNSSLVQLHILEGPE--SSSGAGNFMPSWKYWLQLPFFCAFPKLIELYKDNTNISLGFSIVGGIDS--ANNTNHALSFWD-------TCIMIKTILPGSIAFINGKLKCGDILLEVNNVSLSHISHADAVLCLKKVTGRVHLKIISWPGTIV 818 The following BLAST results are available for this feature:
BLAST of Ligand of Numb protein X 2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Ligand of Numb protein X 2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Ligand of Numb protein X 2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Ligand of Numb protein X 2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Ligand of Numb protein X 2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Ligand of Numb protein X 2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Ligand of Numb protein X 2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Ligand of Numb protein X 2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Ligand of Numb protein X 2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Ligand of Numb protein X 2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Ligand of Numb protein X 2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Ligand of Numb protein X 2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Ligand of Numb protein X 2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Ligand of Numb protein X 2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30024655 ID=SMED30024655|Name=Ligand of Numb protein X 2|organism=Schmidtea mediterranea sexual|type=transcript|length=2153bpback to top protein sequence of SMED30024655-orf-1 >SMED30024655-orf-1 ID=SMED30024655-orf-1|Name=SMED30024655-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=629bp MSIDPKIKSKFSSFKNDDRLMSVTVTSSPCQEFIPKSEIDPNLIKQCQGLback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|
