RRM domain-containing protein

Overview


Name	RRM domain-containing protein
Smed ID	SMED30024648
Length (bp)	438

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of RRM domain-containing protein (SMED30024648) t-SNE clustered cells

Violin plots show distribution of expression levels for RRM domain-containing protein (SMED30024648) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of RRM domain-containing protein (SMED30024648) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for RRM domain-containing protein (SMED30024648) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30024648

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 7

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
tail region	SMED30024648	SMESG000040762.1	dd_Smed_v4_1672_0_1	dd_Smed_v4	PMID:27063937 Scimone et al., 2016
head region	SMED30024648		SmedASXL_067061	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
nervous system	SMED30024648	SMESG000040762.1	dd_Smed_v4_1672_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
pharynx	SMED30024648		dd_Smed_v4_11998_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30024648		dd_Smed_v4_11998_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30024648		dd_Smed_v4_11998_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
head region	SMED30024648		dd_Smed_v6_11998_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Human
Match: SF3B6 (splicing factor 3b subunit 6 [Source:HGNC Symbol;Acc:HGNC:30096])

HSP 1 Score: 114.005 bits (284), Expect = 6.998e-33
Identity = 49/93 (52.69%), Postives = 73/93 (78.49%), Query Frame = 2

Query:   26 IAMAQSKK-KIQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFT 301
            +AM  +K+  I+LPPEV+++L I+NLP ++  +E+Y IF KYG IRQIR+G + +T+GT +VV+E+I DAK ACDHLSGF + + Y+VV+Y+ 
Sbjct:    1 MAMQAAKRANIRLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYN 93

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Human
Match: SNRPA (small nuclear ribonucleoprotein polypeptide A [Source:HGNC Symbol;Acc:HGNC:11151])

HSP 1 Score: 44.2838 bits (103), Expect = 3.159e-6
Identity = 23/68 (33.82%), Postives = 35/68 (51.47%), Query Frame = 2

Query:   83 LLIKNLPNQMQKDEL----YTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMD 274
            + I NL  +++KDEL    Y IF ++G I  I +  S   +G  FV+F+ +  A  A   + GF   D
Sbjct:   12 IYINNLNEKIKKDELKKSLYAIFSQFGQILDILVSRSLKMRGQAFVIFKEVSSATNALRSMQGFPFYD 79

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Human
Match: SNRPA (small nuclear ribonucleoprotein polypeptide A [Source:HGNC Symbol;Acc:HGNC:11151])

HSP 1 Score: 45.0542 bits (105), Expect = 4.926e-6
Identity = 24/75 (32.00%), Postives = 38/75 (50.67%), Query Frame = 2

Query:   83 LLIKNLPNQMQKDEL----YTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVY 295
            + I NL  +++KDEL    Y IF ++G I  I +  S   +G  FV+F+ +  A  A   + GF   D  + + Y
Sbjct:   12 IYINNLNEKIKKDELKKSLYAIFSQFGQILDILVSRSLKMRGQAFVIFKEVSSATNALRSMQGFPFYDKPMRIQY 86

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Human
Match: SNRPA (small nuclear ribonucleoprotein polypeptide A [Source:HGNC Symbol;Acc:HGNC:11151])

HSP 1 Score: 45.0542 bits (105), Expect = 5.285e-6
Identity = 24/75 (32.00%), Postives = 38/75 (50.67%), Query Frame = 2

Query:   83 LLIKNLPNQMQKDEL----YTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVY 295
            + I NL  +++KDEL    Y IF ++G I  I +  S   +G  FV+F+ +  A  A   + GF   D  + + Y
Sbjct:   12 IYINNLNEKIKKDELKKSLYAIFSQFGQILDILVSRSLKMRGQAFVIFKEVSSATNALRSMQGFPFYDKPMRIQY 86

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Human
Match: SNRPA (small nuclear ribonucleoprotein polypeptide A [Source:HGNC Symbol;Acc:HGNC:11151])

HSP 1 Score: 45.4394 bits (106), Expect = 7.503e-6
Identity = 24/75 (32.00%), Postives = 38/75 (50.67%), Query Frame = 2

Query:   83 LLIKNLPNQMQKDEL----YTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVY 295
            + I NL  +++KDEL    Y IF ++G I  I +  S   +G  FV+F+ +  A  A   + GF   D  + + Y
Sbjct:   12 IYINNLNEKIKKDELKKSLYAIFSQFGQILDILVSRSLKMRGQAFVIFKEVSSATNALRSMQGFPFYDKPMRIQY 86

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Celegans
Match: C50D2.5 (Splicing factor 3B subunit 6-like protein [Source:UniProtKB/Swiss-Prot;Acc:Q8ITY4])

HSP 1 Score: 114.39 bits (285), Expect = 2.571e-33
Identity = 49/99 (49.49%), Postives = 77/99 (77.78%), Query Frame = 2

Query:   26 IAMAQSKKK-IQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFTPYKIIK 319
            +AMA  + +  +LPPEV+++L IKNLP ++  +E+Y IF K+G++RQIR+G +++T+GT FVV+E+I DAKTAC+HLSG+ + + Y+VV+Y+   K  K
Sbjct:    1 MAMANRQNRGAKLPPEVNRILYIKNLPYKITTEEMYEIFGKFGAVRQIRVGNTAETRGTAFVVYEDIFDAKTACEHLSGYNVSNRYLVVLYYQATKAWK 99

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Fly
Match: Sf3b6 (gene:FBgn0035692 transcript:FBtr0077015)

HSP 1 Score: 115.546 bits (288), Expect = 7.807e-34
Identity = 48/92 (52.17%), Postives = 71/92 (77.17%), Query Frame = 2

Query:   44 KKKIQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFTPYKIIK 319
            +  I+LPPEV+++L ++NLP ++  DE+Y IF K+G+IRQIR+G + +T+GT FVV+E+I DAK ACDHLSGF + + Y+VV+Y+   K  K
Sbjct:    4 RNHIRLPPEVNRLLYVRNLPYKITSDEMYDIFGKFGAIRQIRVGNTPETRGTAFVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYQSNKAFK 95

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Fly
Match: elav (gene:FBgn0260400 transcript:FBtr0307173)

HSP 1 Score: 45.8246 bits (107), Expect = 3.088e-6
Identity = 38/111 (34.23%), Postives = 54/111 (48.65%), Query Frame = 2

Query:   83 LLIKNLPNQMQKDELYTIFRKYGSIRQIRL----GESSDTKGTGFVVFENILDAKTACDHLSGFKLMD-NYIVVVYFTPYKIIKVNTPGSLS--IKHQLPSALPPAISKII 394
            L +  LP  M + EL  IF  +G+I   R+    G  + TKG GF+ F+   +A  A   L+G         +VV F+       NTPGS S  I+ QLP+ L P + + I
Sbjct:  250 LYVSGLPKTMTQQELEAIFAPFGAIITSRILQNAGNDTQTKGVGFIRFDKREEATRAIIALNGTTPSSCTDPIVVKFS-------NTPGSTSKIIQPQLPAFLNPQLVRRI 353

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Fly
Match: elav (gene:FBgn0260400 transcript:FBtr0070091)

HSP 1 Score: 45.8246 bits (107), Expect = 3.088e-6
Identity = 38/111 (34.23%), Postives = 54/111 (48.65%), Query Frame = 2

Query:   83 LLIKNLPNQMQKDELYTIFRKYGSIRQIRL----GESSDTKGTGFVVFENILDAKTACDHLSGFKLMD-NYIVVVYFTPYKIIKVNTPGSLS--IKHQLPSALPPAISKII 394
            L +  LP  M + EL  IF  +G+I   R+    G  + TKG GF+ F+   +A  A   L+G         +VV F+       NTPGS S  I+ QLP+ L P + + I
Sbjct:  250 LYVSGLPKTMTQQELEAIFAPFGAIITSRILQNAGNDTQTKGVGFIRFDKREEATRAIIALNGTTPSSCTDPIVVKFS-------NTPGSTSKIIQPQLPAFLNPQLVRRI 353

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Fly
Match: elav (gene:FBgn0260400 transcript:FBtr0100254)

HSP 1 Score: 45.8246 bits (107), Expect = 3.143e-6
Identity = 38/111 (34.23%), Postives = 54/111 (48.65%), Query Frame = 2

Query:   83 LLIKNLPNQMQKDELYTIFRKYGSIRQIRL----GESSDTKGTGFVVFENILDAKTACDHLSGFKLMD-NYIVVVYFTPYKIIKVNTPGSLS--IKHQLPSALPPAISKII 394
            L +  LP  M + EL  IF  +G+I   R+    G  + TKG GF+ F+   +A  A   L+G         +VV F+       NTPGS S  I+ QLP+ L P + + I
Sbjct:  246 LYVSGLPKTMTQQELEAIFAPFGAIITSRILQNAGNDTQTKGVGFIRFDKREEATRAIIALNGTTPSSCTDPIVVKFS-------NTPGSTSKIIQPQLPAFLNPQLVRRI 349

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Fly
Match: elav (gene:FBgn0260400 transcript:FBtr0114366)

HSP 1 Score: 45.8246 bits (107), Expect = 3.143e-6
Identity = 38/111 (34.23%), Postives = 54/111 (48.65%), Query Frame = 2

Query:   83 LLIKNLPNQMQKDELYTIFRKYGSIRQIRL----GESSDTKGTGFVVFENILDAKTACDHLSGFKLMD-NYIVVVYFTPYKIIKVNTPGSLS--IKHQLPSALPPAISKII 394
            L +  LP  M + EL  IF  +G+I   R+    G  + TKG GF+ F+   +A  A   L+G         +VV F+       NTPGS S  I+ QLP+ L P + + I
Sbjct:  246 LYVSGLPKTMTQQELEAIFAPFGAIITSRILQNAGNDTQTKGVGFIRFDKREEATRAIIALNGTTPSSCTDPIVVKFS-------NTPGSTSKIIQPQLPAFLNPQLVRRI 349

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Zebrafish
Match: sf3b6 (splicing factor 3b, subunit 6 [Source:ZFIN;Acc:ZDB-GENE-040625-36])

HSP 1 Score: 114.005 bits (284), Expect = 4.452e-33
Identity = 49/93 (52.69%), Postives = 73/93 (78.49%), Query Frame = 2

Query:   26 IAMAQSKK-KIQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFT 301
            +AM  +K+  I+LPPEV+++L I+NLP ++  +E+Y IF KYG IRQIR+G + +T+GT +VV+E+I DAK ACDHLSGF + + Y+VV+Y+ 
Sbjct:    1 MAMQAAKRANIRLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYN 93

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Zebrafish
Match: snrpa (small nuclear ribonucleoprotein polypeptide A [Source:ZFIN;Acc:ZDB-GENE-030131-2841])

HSP 1 Score: 45.8246 bits (107), Expect = 3.199e-6
Identity = 25/75 (33.33%), Postives = 38/75 (50.67%), Query Frame = 2

Query:   83 LLIKNLPNQMQKDEL----YTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVY 295
            + I NL  +++KDEL    Y IF ++G I  I +  +   KG  FV+F+ I  A  A   + GF   D  + + Y
Sbjct:   12 IYINNLNEKIKKDELKKSLYAIFSQFGQILDILVSRTLKMKGQAFVIFKEINSASNALRSMQGFPFYDKPMRIQY 86

[Close]

Query:   83 LLIKNLPNQMQKDEL----YTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVY 295
            + I NL  +++KDEL    Y IF ++G I  I +  +   KG  FV+F+ I  A  A   + GF   D  + + Y
Sbjct:   12 IYINNLNEKIKKDELKKSLYAIFSQFGQILDILVSRTLKMKGQAFVIFKEINSASNALRSMQGFPFYDKPMRIQY 86

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Zebrafish
Match: pabpc1b (poly A binding protein, cytoplasmic 1 b [Source:ZFIN;Acc:ZDB-GENE-050308-1])

HSP 1 Score: 45.8246 bits (107), Expect = 4.815e-6
Identity = 27/69 (39.13%), Postives = 38/69 (55.07%), Query Frame = 2

Query:   89 IKNLPNQMQKDELYTIFRKYGSIRQIRL--GESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVV 289
            IKN    M  D+L  IF KYG+   IR+   E+  ++G GFV FE   DA+ A D ++G ++    I V
Sbjct:  195 IKNFGEDMDDDKLKDIFSKYGNAMSIRVMTDENGKSRGFGFVSFERHEDAQRAVDEMNGKEMNGKLIYV 263

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Zebrafish
Match: snrpa (small nuclear ribonucleoprotein polypeptide A [Source:ZFIN;Acc:ZDB-GENE-030131-2841])

HSP 1 Score: 45.4394 bits (106), Expect = 4.951e-6
Identity = 25/75 (33.33%), Postives = 38/75 (50.67%), Query Frame = 2

Query:   83 LLIKNLPNQMQKDEL----YTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVY 295
            + I NL  +++KDEL    Y IF ++G I  I +  +   KG  FV+F+ I  A  A   + GF   D  + + Y
Sbjct:   12 IYINNLNEKIKKDELKKSLYAIFSQFGQILDILVSRTLKMKGQAFVIFKEINSASNALRSMQGFPFYDKPMRIQY 86

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Xenopus
Match: sf3b6 (splicing factor 3b subunit 6 [Source:NCBI gene;Acc:550003])

HSP 1 Score: 113.62 bits (283), Expect = 9.808e-33
Identity = 49/93 (52.69%), Postives = 73/93 (78.49%), Query Frame = 2

Query:   26 IAMAQSKK-KIQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFT 301
            +AM  +K+  I+LPPEV+++L I+NLP ++  +E+Y IF KYG IRQIR+G + +T+GT +VV+E+I DAK ACDHLSGF + + Y+VV+Y+ 
Sbjct:    1 MAMQAAKRANIRLPPEVNRILYIRNLPYKITGEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYN 93

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Xenopus
Match: sf3b6 (splicing factor 3b subunit 6 [Source:NCBI gene;Acc:550003])

HSP 1 Score: 112.079 bits (279), Expect = 3.170e-32
Identity = 47/85 (55.29%), Postives = 68/85 (80.00%), Query Frame = 2

Query:   47 KKIQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFT 301
            K I+LPPEV+++L I+NLP ++  +E+Y IF KYG IRQIR+G + +T+GT +VV+E+I DAK ACDHLSGF + + Y+VV+Y+ 
Sbjct:    7 KGIRLPPEVNRILYIRNLPYKITGEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYN 91

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Xenopus
Match: tubgcp5 (tubulin gamma complex associated protein 5 [Source:Xenbase;Acc:XB-GENE-494053])

HSP 1 Score: 45.8246 bits (107), Expect = 5.376e-6
Identity = 25/75 (33.33%), Postives = 38/75 (50.67%), Query Frame = 2

Query:   83 LLIKNLPNQMQKDEL----YTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVY 295
            + I NL  +++KDEL    Y IF ++G I  I +  S   +G  FV+F+ I  A  A   + GF   D  + + Y
Sbjct:   16 IYINNLNEKIKKDELKKSLYAIFSQFGQILDILVSRSLKMRGQAFVIFKEISSATNALRSMQGFPFYDKPMRIQY 90

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Xenopus
Match: tubgcp5 (tubulin gamma complex associated protein 5 [Source:Xenbase;Acc:XB-GENE-494053])

HSP 1 Score: 45.8246 bits (107), Expect = 5.397e-6
Identity = 25/75 (33.33%), Postives = 38/75 (50.67%), Query Frame = 2

Query:   83 LLIKNLPNQMQKDEL----YTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVY 295
            + I NL  +++KDEL    Y IF ++G I  I +  S   +G  FV+F+ I  A  A   + GF   D  + + Y
Sbjct:   12 IYINNLNEKIKKDELKKSLYAIFSQFGQILDILVSRSLKMRGQAFVIFKEISSATNALRSMQGFPFYDKPMRIQY 86

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Mouse
Match: Sf3b6 (splicing factor 3B, subunit 6 [Source:MGI Symbol;Acc:MGI:1913305])

HSP 1 Score: 114.005 bits (284), Expect = 4.804e-33
Identity = 49/93 (52.69%), Postives = 73/93 (78.49%), Query Frame = 2

Query:   26 IAMAQSKK-KIQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFT 301
            +AM  +K+  I+LPPEV+++L I+NLP ++  +E+Y IF KYG IRQIR+G + +T+GT +VV+E+I DAK ACDHLSGF + + Y+VV+Y+ 
Sbjct:    1 MAMQAAKRANIRLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYN 93

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Mouse
Match: Sf3b6 (splicing factor 3B, subunit 6 [Source:MGI Symbol;Acc:MGI:1913305])

HSP 1 Score: 111.309 bits (277), Expect = 3.295e-32
Identity = 46/83 (55.42%), Postives = 67/83 (80.72%), Query Frame = 2

Query:   53 IQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFT 301
            I+LPPEV+++L I+NLP ++  +E+Y IF KYG IRQIR+G + +T+GT +VV+E+I DAK ACDHLSGF + + Y+VV+Y+ 
Sbjct:    1 IRLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYN 83

[Close]

BLAST of RRM domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9VRV7|SF3B6_DROME (Splicing factor 3B subunit 6 OS=Drosophila melanogaster OX=7227 GN=Sf3b6 PE=1 SV=1)

HSP 1 Score: 115.546 bits (288), Expect = 7.826e-33
Identity = 48/92 (52.17%), Postives = 71/92 (77.17%), Query Frame = 2

Query:   44 KKKIQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFTPYKIIK 319
            +  I+LPPEV+++L ++NLP ++  DE+Y IF K+G+IRQIR+G + +T+GT FVV+E+I DAK ACDHLSGF + + Y+VV+Y+   K  K
Sbjct:    4 RNHIRLPPEVNRLLYVRNLPYKITSDEMYDIFGKFGAIRQIRVGNTPETRGTAFVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYQSNKAFK 95

[Close]

BLAST of RRM domain-containing protein vs. UniProt/SwissProt
Match: sp|Q8ITY4|SF3B6_CAEEL (Splicing factor 3B subunit 6-like protein OS=Caenorhabditis elegans OX=6239 GN=C50D2.5 PE=3 SV=2)

HSP 1 Score: 114.39 bits (285), Expect = 3.271e-32
Identity = 49/99 (49.49%), Postives = 77/99 (77.78%), Query Frame = 2

Query:   26 IAMAQSKKK-IQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFTPYKIIK 319
            +AMA  + +  +LPPEV+++L IKNLP ++  +E+Y IF K+G++RQIR+G +++T+GT FVV+E+I DAKTAC+HLSG+ + + Y+VV+Y+   K  K
Sbjct:    1 MAMANRQNRGAKLPPEVNRILYIKNLPYKITTEEMYEIFGKFGAVRQIRVGNTAETRGTAFVVYEDIFDAKTACEHLSGYNVSNRYLVVLYYQATKAWK 99

[Close]

BLAST of RRM domain-containing protein vs. UniProt/SwissProt
Match: sp|P59708|SF3B6_MOUSE (Splicing factor 3B subunit 6 OS=Mus musculus OX=10090 GN=Sf3b6 PE=1 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 3.360e-32
Identity = 49/93 (52.69%), Postives = 73/93 (78.49%), Query Frame = 2

Query:   26 IAMAQSKK-KIQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFT 301
            +AM  +K+  I+LPPEV+++L I+NLP ++  +E+Y IF KYG IRQIR+G + +T+GT +VV+E+I DAK ACDHLSGF + + Y+VV+Y+ 
Sbjct:    1 MAMQAAKRANIRLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYN 93

[Close]

BLAST of RRM domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9Y3B4|SF3B6_HUMAN (Splicing factor 3B subunit 6 OS=Homo sapiens OX=9606 GN=SF3B6 PE=1 SV=1)

HSP 1 Score: 114.005 bits (284), Expect = 3.360e-32
Identity = 49/93 (52.69%), Postives = 73/93 (78.49%), Query Frame = 2

Query:   26 IAMAQSKK-KIQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFT 301
            +AM  +K+  I+LPPEV+++L I+NLP ++  +E+Y IF KYG IRQIR+G + +T+GT +VV+E+I DAK ACDHLSGF + + Y+VV+Y+ 
Sbjct:    1 MAMQAAKRANIRLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYN 93

[Close]

BLAST of RRM domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9FMP4|SF3B6_ARATH (Splicing factor 3B subunit 6-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g12190 PE=2 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 4.045e-29
Identity = 47/92 (51.09%), Postives = 67/92 (72.83%), Query Frame = 2

Query:   44 KKKIQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFTPYKIIK 319
            K   +LPPEV+++L ++NLP  +  +E+Y IF KYG+IRQIR+G    TKGT FVV+E+I DAK A DHLSGF + + Y++V+Y+   K+ K
Sbjct:    8 KSNTRLPPEVNRVLYVRNLPFNITSEEMYDIFGKYGAIRQIRIGCDKATKGTAFVVYEDIYDAKNAVDHLSGFNVANRYLIVLYYQHAKMSK 99

[Close]

BLAST of RRM domain-containing protein vs. TrEMBL
Match: A0A1S3D7L5 (splicing factor 3B subunit 6 OS=Diaphorina citri OX=121845 GN=LOC103513105 PE=4 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 2.547e-35
Identity = 54/98 (55.10%), Postives = 77/98 (78.57%), Query Frame = 2

Query:   26 IAMAQSKKKIQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFTPYKIIK 319
            +AM+Q +  ++LPPEV+++L I+NLP ++  DE+Y IF KYG+IRQIR+G + DT+GT FVV+E+I DAK ACDHLSGF + + Y+VV+Y+ P K  K
Sbjct:    1 MAMSQRRANVRLPPEVNRVLYIRNLPYKITGDEMYDIFGKYGAIRQIRIGNTPDTRGTAFVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYQPNKAFK 98

[Close]

BLAST of RRM domain-containing protein vs. TrEMBL
Match: A0A1Y2BD33 (RNA-binding domain-containing protein OS=Neocallimastix californiae OX=1754190 GN=LY90DRAFT_705195 PE=4 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 1.411e-34
Identity = 53/95 (55.79%), Postives = 75/95 (78.95%), Query Frame = 2

Query:   35 AQSKKKIQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFTPYKIIK 319
            A+++  I+LPPEV+++L ++NLP ++  DELY +F KYG+IRQIRLG ++DT+GT FVV+E+I DAK ACDHLSGF +M  Y++V+Y  P K  K
Sbjct:    5 AKARANIRLPPEVNRILFVRNLPFKITSDELYDLFGKYGAIRQIRLGNANDTRGTAFVVYEDIFDAKAACDHLSGFNIMGRYLIVLYHQPNKTHK 99

[Close]

BLAST of RRM domain-containing protein vs. TrEMBL
Match: A0A267DPF7 (RRM domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig028462g2 PE=4 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 1.528e-34
Identity = 57/105 (54.29%), Postives = 82/105 (78.10%), Query Frame = 2

Query:    8 CLQQSPIAMAQSKK-KIQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFTPYKIIK 319
            CL + P +MA SK+  ++LPPEV+++LL+KNLP  +  +E+Y IF KYG+IRQIR+G + +T+GT FVV+E+I DAK ACDHLSGF + + Y+VV+Y+ P K+ K
Sbjct:    1 CLSKQP-SMALSKRLNVRLPPEVNRILLVKNLPFSISAEEMYDIFGKYGAIRQIRVGNTPETRGTAFVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYQPTKVQK 104

[Close]

BLAST of RRM domain-containing protein vs. TrEMBL
Match: A0A1Y2AFM2 (RNA-binding domain-containing protein OS=Neocallimastix californiae OX=1754190 GN=LY90DRAFT_390703 PE=4 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 2.281e-34
Identity = 52/95 (54.74%), Postives = 75/95 (78.95%), Query Frame = 2

Query:   35 AQSKKKIQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFTPYKIIK 319
            A+++  ++LPPEV+++L ++NLP ++  DELY +F KYG+IRQIRLG ++DT+GT FVV+E+I DAK ACDHLSGF +M  Y++V+Y  P K  K
Sbjct:    5 AKARANVRLPPEVNRILFVRNLPFKITSDELYDLFGKYGAIRQIRLGNANDTRGTAFVVYEDIFDAKAACDHLSGFNIMGRYLIVLYHQPNKTHK 99

[Close]

BLAST of RRM domain-containing protein vs. TrEMBL
Match: K1QS87 (Pre-mRNA branch site p14-like protein OS=Crassostrea gigas OX=29159 GN=CGI_10024678 PE=4 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 3.793e-34
Identity = 54/102 (52.94%), Postives = 79/102 (77.45%), Query Frame = 2

Query:   14 QQSPIAMAQSKKKIQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFTPYKIIK 319
            ++S I  A +  K++LPPEV+++L ++NLP ++  +E+Y IF KYG+IRQIR+G +++TKGT FVV+E+I DAK ACDHLSGF + + Y+VV+YF P K  K
Sbjct:    9 EESRIKNAIASAKVRLPPEVNRILYVRNLPYKITAEEMYDIFGKYGAIRQIRVGNTTETKGTAFVVYEDIFDAKNACDHLSGFNVCNRYLVVLYFQPNKAFK 110

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Cavefish
Match: sf3b6 (splicing factor 3b subunit 6 [Source:NCBI gene;Acc:103024112])

HSP 1 Score: 114.005 bits (284), Expect = 3.773e-33
Identity = 49/93 (52.69%), Postives = 73/93 (78.49%), Query Frame = 2

Query:   26 IAMAQSKK-KIQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFT 301
            +AM  +K+  I+LPPEV+++L I+NLP ++  +E+Y IF KYG IRQIR+G + +T+GT +VV+E+I DAK ACDHLSGF + + Y+VV+Y+ 
Sbjct:    1 MAMQAAKRANIRLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYN 93

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000018970.2 (polyadenylate-binding protein 1-like [Source:NCBI gene;Acc:103030422])

HSP 1 Score: 45.0542 bits (105), Expect = 8.034e-6
Identity = 27/69 (39.13%), Postives = 37/69 (53.62%), Query Frame = 2

Query:   89 IKNLPNQMQKDELYTIFRKYGSIRQIRL--GESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVV 289
            IKN    M  ++L  +F KYG    IR+   ES  +KG GFV FE   DA+ A D ++G +L    + V
Sbjct:  195 IKNFGEDMDDEKLKDVFGKYGPALSIRVMTDESGKSKGFGFVSFERHEDAQRAVDEMNGKELNGKQVYV 263

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006657.1 (pep scaffold:Pmarinus_7.0:GL484402:615:2775:1 gene:ENSPMAG00000006008.1 transcript:ENSPMAT00000006657.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 113.62 bits (283), Expect = 9.110e-34
Identity = 44/93 (47.31%), Postives = 75/93 (80.65%), Query Frame = 2

Query:   26 IAMAQSKKKIQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFTP 304
            ++++  + +++LPPEV+++L ++NLP ++  +E+Y IF KYG+IRQIR+G + +++GT +VV+E+I DAK ACDHLSGF + + Y+VV+Y+ P
Sbjct:    2 VSVSCVRVQVRLPPEVNRILYVRNLPYKITAEEMYDIFGKYGAIRQIRVGTTPESRGTAYVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYNP 94

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Yeast
Match: NSR1 (Nucleolar protein that binds nuclear localization sequences; required for pre-rRNA processing and ribosome biogenesis; binds to single stranded telomeric DNA and mRNA; methylated by Hmt1p; interaction with Top1p and nucleolar localization are negatively regulated by polyphosphorylation [Source:SGD;Acc:S000003391])

HSP 1 Score: 51.2174 bits (121), Expect = 6.579e-9
Identity = 31/91 (34.07%), Postives = 49/91 (53.85%), Query Frame = 2

Query:   38 QSKKKIQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLG---ESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFT 301
            ++KK    P E S  L + NL     +D ++ +F K+G +  +R+    E+   KG G+V F N+ DAK A D L G + +DN  V + F+
Sbjct:  254 RAKKFGDTPSEPSDTLFLGNLSFNADRDAIFELFAKHGEVVSVRIPTHPETEQPKGFGYVQFSNMEDAKKALDALQG-EYIDNRPVRLDFS 343

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Yeast
Match: MRD1 (Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region [Source:SGD;Acc:S000006316])

HSP 1 Score: 44.2838 bits (103), Expect = 1.716e-6
Identity = 21/76 (27.63%), Postives = 42/76 (55.26%), Query Frame = 2

Query:   74 SKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENIL--DAKTACDHLSGFKLMDNYIVVVY 295
            S  +++KNLP +  + +++ +F  +G ++ +R+ +  D    GF   E +L  +A+ A D L G  L+   +V+ Y
Sbjct:  762 SGKIIVKNLPFEATRKDVFELFNSFGQLKSVRVPKKFDKSARGFAFVEFLLPKEAENAMDQLHGVHLLGRRLVMQY 837

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Nematostella
Match: EDO36652 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SI29])

HSP 1 Score: 119.013 bits (297), Expect = 1.571e-35
Identity = 51/95 (53.68%), Postives = 72/95 (75.79%), Query Frame = 2

Query:   35 AQSKKKIQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFTPYKIIK 319
            +Q K  ++LPPEV+++L I+NLP ++  +E+Y IF KYG+IRQIRLG + DT+GT FVV+E+I DAK ACDHLSGF +   Y+VV+Y+   K  +
Sbjct:    5 SQRKANVRLPPEVNRILYIRNLPYKITAEEMYDIFGKYGAIRQIRLGNAPDTRGTAFVVYEDIFDAKNACDHLSGFNVCGRYLVVLYYQSNKAFQ 99

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Medaka
Match: sf3b6 (splicing factor 3b subunit 6 [Source:NCBI gene;Acc:101163944])

HSP 1 Score: 112.079 bits (279), Expect = 2.143e-32
Identity = 48/93 (51.61%), Postives = 72/93 (77.42%), Query Frame = 2

Query:   26 IAMAQSKK-KIQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFT 301
            +AM  +K+  I+LPPEV+++L I+NLP ++  +E+Y IF KYG IRQIR G + +++GT +VV+E+I DAK ACDHLSGF + + Y+VV+Y+ 
Sbjct:    1 MAMQAAKRANIRLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRTGNTPESRGTAYVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYN 93

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Medaka
Match: cirbpa (cold inducible RNA binding protein [Source:NCBI gene;Acc:101165004])

HSP 1 Score: 46.595 bits (109), Expect = 9.670e-7
Identity = 27/73 (36.99%), Postives = 41/73 (56.16%), Query Frame = 2

Query:   83 LLIKNLPNQMQKDELYTIFRKYGSIRQ---IRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVV 292
            L I  L  +  +D L   F KYG+I +   IR  E+  ++G GFV ++N+ DAK A D ++G K +D   + V
Sbjct:    6 LFIGGLSFETNEDSLAAAFGKYGTIEKVDVIRDKETGRSRGFGFVKYDNVEDAKDALDAMNG-KTLDGRAIRV 77

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Medaka
Match: cirbpa (cold inducible RNA binding protein [Source:NCBI gene;Acc:101165004])

HSP 1 Score: 46.2098 bits (108), Expect = 1.535e-6
Identity = 27/73 (36.99%), Postives = 41/73 (56.16%), Query Frame = 2

Query:   83 LLIKNLPNQMQKDELYTIFRKYGSIRQ---IRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVV 292
            L I  L  +  +D L   F KYG+I +   IR  E+  ++G GFV ++N+ DAK A D ++G K +D   + V
Sbjct:    7 LFIGGLSFETNEDSLAAAFGKYGTIEKVDVIRDKETGRSRGFGFVKYDNVEDAKDALDAMNG-KTLDGRAIRV 78

[Close]

BLAST of RRM domain-containing protein vs. Ensembl Medaka
Match: snrpa (small nuclear ribonucleoprotein polypeptide A [Source:NCBI gene;Acc:101155621])

HSP 1 Score: 46.595 bits (109), Expect = 1.588e-6
Identity = 28/83 (33.73%), Postives = 42/83 (50.60%), Query Frame = 2

Query:   65 PEV--SKMLLIKNLPNQMQKDEL----YTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVY 295
            PEV  +  + I NL  +++KDEL    Y IF ++G I  I +  +   KG  FV+F+ I  A  A   + GF   D  + + Y
Sbjct:    4 PEVRPNHTIYINNLNEKIKKDELKKSLYAIFSQFGQILDILVARTLKMKGQAFVIFKEINSASNALRSMQGFPFYDKPMRIQY 86

[Close]

Query:   65 PEV--SKMLLIKNLPNQMQKDEL----YTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVY 295
            PEV  +  + I NL  +++KDEL    Y IF ++G I  I +  +   KG  FV+F+ I  A  A   + GF   D  + + Y
Sbjct:    4 PEVRPNHTIYINNLNEKIKKDELKKSLYAIFSQFGQILDILVARTLKMKGQAFVIFKEINSASNALRSMQGFPFYDKPMRIQY 86

[Close]

BLAST of RRM domain-containing protein vs. Planmine SMEST
Match: SMESG000081357.1 (SMESG000081357.1)

HSP 1 Score: 113.235 bits (282), Expect = 4.989e-33
Identity = 49/97 (50.52%), Postives = 73/97 (75.26%), Query Frame = 2

Query:   32 MAQSKKKI-QLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFTPYKIIK 319
            MA S K+I +LPP VS++L++ NLP +++ DE+Y IF KYG+IRQIR+G + + KG  FVV+E+I DA+ AC+HL+GF + + YI + Y+ P K+ K
Sbjct:    1 MAFSNKRITKLPPNVSRILMVSNLPYELRDDEMYDIFGKYGTIRQIRVGNTPENKGKAFVVYEDIYDAQNACEHLTGFNVCNRYICISYYQPNKVFK 97

[Close]

BLAST of RRM domain-containing protein vs. Planmine SMEST
Match: SMESG000078309.1 (SMESG000078309.1)

HSP 1 Score: 48.1358 bits (113), Expect = 4.984e-8
Identity = 25/71 (35.21%), Postives = 40/71 (56.34%), Query Frame = 2

Query:   65 PEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLG---ESSDTKGTGFVVFENILDAKTACDHLSGFKL 268
            P  +  + + N+P  +++DEL  IF +YG+I    L     S   KG  FV FE+I DA+ + D L G+++
Sbjct:    2 PRSTTTIYVGNIPEDLRRDELKRIFGRYGNIVAATLPVDYYSGIPKGFAFVQFEDIRDAEESFDRLQGYRI 72

[Close]

BLAST of RRM domain-containing protein vs. Planmine SMEST
Match: SMESG000016033.1 (SMESG000016033.1)

HSP 1 Score: 46.9802 bits (110), Expect = 3.996e-7
Identity = 30/94 (31.91%), Postives = 48/94 (51.06%), Query Frame = 2

Query:   83 LLIKNLPNQMQKDELYTIFRKYGSIRQIRLG---ESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFTPYKIIKVNTPGSLSIKHQ 355
            L ++NL ++++ ++L  +F KYG I  I++     S   KG GF  FE+  DA+ A   L G +L+   I V +   YK     TP  +  K +
Sbjct:    9 LFVRNLSDRVRYEDLEKLFGKYGRIYNIKIPLDYYSGRMKGFGFCTFEDFRDAEDAKTRLDGAQLLGREIGVEFTNGYK----KTPAEMRDKEK 98

[Close]

BLAST of RRM domain-containing protein vs. Planmine SMEST
Match: SMESG000058799.1 (SMESG000058799.1)

HSP 1 Score: 48.1358 bits (113), Expect = 5.802e-7
Identity = 35/115 (30.43%), Postives = 59/115 (51.30%), Query Frame = 2

Query:   17 QSPIAMAQSKKKIQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTK---GTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFTPYKIIKVNTPGSLSIKH 352
            Q     AQ K K     + S++ L +NL   + ++ LY +F  YG I  IRL  +S+T+   G  ++ F    DAKTA D ++  K+  N   + +    K+I+ +  G++ IK+
Sbjct:  560 QQKGTFAQQKTKPSWYSDDSEIFL-ENLHPDVDEEFLYKLFHSYGEILSIRLMRNSETQKSLGVAYITFVRRSDAKTAMDKVNFKKIYRNECRIKWSESNKVIRKSEIGNVFIKN 673

[Close]

BLAST of RRM domain-containing protein vs. Planmine SMEST
Match: SMESG000058799.1 (SMESG000058799.1)

HSP 1 Score: 47.7506 bits (112), Expect = 5.843e-7
Identity = 34/111 (30.63%), Postives = 58/111 (52.25%), Query Frame = 2

Query:   29 AMAQSKKKIQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYGSIRQIRLGESSDTK---GTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFTPYKIIKVNTPGSLSIKH 352
              AQ K K     + S++ L +NL   + ++ LY +F  YG I  IRL  +S+T+   G  ++ F    DAKTA D ++  K+  N   + +    K+I+ +  G++ IK+
Sbjct:  564 TFAQQKTKPSWYSDDSEIFL-ENLHPDVDEEFLYKLFHSYGEILSIRLMRNSETQKSLGVAYITFVRRSDAKTAMDKVNFKKIYRNECRIKWSESNKVIRKSEIGNVFIKN 673

The following BLAST results are available for this feature:

BLAST of RRM domain-containing protein vs. Ensembl Human
BLAST of RRM domain-containing protein vs. Ensembl Celegans
BLAST of RRM domain-containing protein vs. Ensembl Fly
BLAST of RRM domain-containing protein vs. Ensembl Zebrafish
BLAST of RRM domain-containing protein vs. Ensembl Xenopus
BLAST of RRM domain-containing protein vs. Ensembl Mouse
BLAST of RRM domain-containing protein vs. UniProt/SwissProt
BLAST of RRM domain-containing protein vs. TrEMBL
BLAST of RRM domain-containing protein vs. Ensembl Cavefish
BLAST of RRM domain-containing protein vs. Ensembl Sea Lamprey
BLAST of RRM domain-containing protein vs. Ensembl Yeast
BLAST of RRM domain-containing protein vs. Ensembl Nematostella
BLAST of RRM domain-containing protein vs. Ensembl Medaka
BLAST of RRM domain-containing protein vs. Planmine SMEST

BLAST of RRM domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
SF3B6	6.998e-33	52.69	splicing factor 3b subunit 6 [Source:HGNC Symbol;A...	[more]
SNRPA	3.159e-6	33.82	small nuclear ribonucleoprotein polypeptide A [Sou...	[more]
SNRPA	4.926e-6	32.00	small nuclear ribonucleoprotein polypeptide A [Sou...	[more]
SNRPA	5.285e-6	32.00	small nuclear ribonucleoprotein polypeptide A [Sou...	[more]
SNRPA	7.503e-6	32.00	small nuclear ribonucleoprotein polypeptide A [Sou...	[more]

BLAST of RRM domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
C50D2.5	2.571e-33	49.49	Splicing factor 3B subunit 6-like protein [Source...	[more]

BLAST of RRM domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Sf3b6	7.807e-34	52.17	gene:FBgn0035692 transcript:FBtr0077015	[more]
elav	3.088e-6	34.23	gene:FBgn0260400 transcript:FBtr0307173	[more]
elav	3.088e-6	34.23	gene:FBgn0260400 transcript:FBtr0070091	[more]
elav	3.143e-6	34.23	gene:FBgn0260400 transcript:FBtr0100254	[more]
elav	3.143e-6	34.23	gene:FBgn0260400 transcript:FBtr0114366	[more]

BLAST of RRM domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
sf3b6	4.452e-33	52.69	splicing factor 3b, subunit 6 [Source:ZFIN;Acc:ZDB...	[more]
snrpa	3.199e-6	33.33	small nuclear ribonucleoprotein polypeptide A [Sou...	[more]
snrpa	3.199e-6	33.33	small nuclear ribonucleoprotein polypeptide A [Sou...	[more]
pabpc1b	4.815e-6	39.13	poly A binding protein, cytoplasmic 1 b [Source:ZF...	[more]
snrpa	4.951e-6	33.33	small nuclear ribonucleoprotein polypeptide A [Sou...	[more]

BLAST of RRM domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 4


Match Name	E-value	Identity	Description
sf3b6	9.808e-33	52.69	splicing factor 3b subunit 6 [Source:NCBI gene;Acc...	[more]
sf3b6	3.170e-32	55.29	splicing factor 3b subunit 6 [Source:NCBI gene;Acc...	[more]
tubgcp5	5.376e-6	33.33	tubulin gamma complex associated protein 5 [Source...	[more]
tubgcp5	5.397e-6	33.33	tubulin gamma complex associated protein 5 [Source...	[more]

BLAST of RRM domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2


Match Name	E-value	Identity	Description
Sf3b6	4.804e-33	52.69	splicing factor 3B, subunit 6 [Source:MGI Symbol;A...	[more]
Sf3b6	3.295e-32	55.42	splicing factor 3B, subunit 6 [Source:MGI Symbol;A...	[more]

BLAST of RRM domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q9VRV7\|SF3B6_DROME	7.826e-33	52.17	Splicing factor 3B subunit 6 OS=Drosophila melanog...	[more]
sp\|Q8ITY4\|SF3B6_CAEEL	3.271e-32	49.49	Splicing factor 3B subunit 6-like protein OS=Caeno...	[more]
sp\|P59708\|SF3B6_MOUSE	3.360e-32	52.69	Splicing factor 3B subunit 6 OS=Mus musculus OX=10...	[more]
sp\|Q9Y3B4\|SF3B6_HUMAN	3.360e-32	52.69	Splicing factor 3B subunit 6 OS=Homo sapiens OX=96...	[more]
sp\|Q9FMP4\|SF3B6_ARATH	4.045e-29	51.09	Splicing factor 3B subunit 6-like protein OS=Arabi...	[more]

BLAST of RRM domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A1S3D7L5	2.547e-35	55.10	splicing factor 3B subunit 6 OS=Diaphorina citri O...	[more]
A0A1Y2BD33	1.411e-34	55.79	RNA-binding domain-containing protein OS=Neocallim...	[more]
A0A267DPF7	1.528e-34	54.29	RRM domain-containing protein (Fragment) OS=Macros...	[more]
A0A1Y2AFM2	2.281e-34	54.74	RNA-binding domain-containing protein OS=Neocallim...	[more]
K1QS87	3.793e-34	52.94	Pre-mRNA branch site p14-like protein OS=Crassostr...	[more]

BLAST of RRM domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
sf3b6	3.773e-33	52.69	splicing factor 3b subunit 6 [Source:NCBI gene;Acc...	[more]
ENSAMXT00000018970.2	8.034e-6	39.13	polyadenylate-binding protein 1-like [Source:NCBI ...	[more]

BLAST of RRM domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
ENSPMAT00000006657.1	9.110e-34	47.31	pep scaffold:Pmarinus_7.0:GL484402:615:2775:1 gene...	[more]

BLAST of RRM domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2


Match Name	E-value	Identity	Description
NSR1	6.579e-9	34.07	Nucleolar protein that binds nuclear localization ...	[more]
MRD1	1.716e-6	27.63	Essential conserved small ribosomal subunit (40s) ...	[more]

BLAST of RRM domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO36652	1.571e-35	53.68	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of RRM domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
sf3b6	2.143e-32	51.61	splicing factor 3b subunit 6 [Source:NCBI gene;Acc...	[more]
cirbpa	9.670e-7	36.99	cold inducible RNA binding protein [Source:NCBI ge...	[more]
cirbpa	1.535e-6	36.99	cold inducible RNA binding protein [Source:NCBI ge...	[more]
snrpa	1.588e-6	33.73	small nuclear ribonucleoprotein polypeptide A [Sou...	[more]
snrpa	1.588e-6	33.73	small nuclear ribonucleoprotein polypeptide A [Sou...	[more]

BLAST of RRM domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000081357.1	4.989e-33	50.52	SMESG000081357.1	[more]
SMESG000078309.1	4.984e-8	35.21	SMESG000078309.1	[more]
SMESG000016033.1	3.996e-7	31.91	SMESG000016033.1	[more]
SMESG000058799.1	5.802e-7	30.43	SMESG000058799.1	[more]
SMESG000058799.1	5.843e-7	30.63	SMESG000058799.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30024648 ID=SMED30024648|Name=RRM domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=438bp
GTTGCTTTGTTTACAACAGTCACCTATAGCAATGGCGCAAAGCAAAAAAA
AAATTCAACTGCCTCCAGAAGTTAGTAAGATGCTGCTTATAAAAAATCTT
CCTAATCAAATGCAAAAGGATGAATTATATACGATATTTAGAAAATATGG
ATCAATTAGACAAATTCGATTAGGTGAAAGTTCAGACACTAAGGGAACAG
GATTTGTAGTATTTGAAAACATATTAGATGCTAAAACGGCTTGTGATCAT
CTCAGCGGATTTAAATTAATGGACAATTATATAGTAGTCGTTTACTTCAC
ACCATATAAAATTATTAAAGTAAATACTCCTGGGTCGCTTTCAATTAAAC
ACCAATTACCATCAGCTCTACCGCCAGCCATTTCAAAAATTATAATATTC
AAACAAAAAGTATATTAATAACAAGTAACAGATTCCGG

protein sequence of SMED30024648-orf-1

>SMED30024648-orf-1 ID=SMED30024648-orf-1|Name=SMED30024648-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=139bp
LLCLQQSPIAMAQSKKKIQLPPEVSKMLLIKNLPNQMQKDELYTIFRKYG
SIRQIRLGESSDTKGTGFVVFENILDAKTACDHLSGFKLMDNYIVVVYFT
PYKIIKVNTPGSLSIKHQLPSALPPAISKIIIFKQKVY*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **molecular function**
Term	Definition
GO:0003723	RNA binding
GO:0003676	nucleic acid binding
GO:0000166	nucleotide binding

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000099	neuron
PLANA:0000418	head
PLANA:0000421	tail

Vocabulary: **INTERPRO**
Term	Definition
IPR034150	SF3B6_RRM
IPR035979	RBD_domain_sf
IPR000504	RRM_dom
IPR012677	Nucleotide-bd_a/b_plait_sf

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000504	RNA recognition motif domain	SMART	SM00360	rrm1_1	coord: 27..97 e-value: 2.0E-13 score: 60.6
IPR000504	RNA recognition motif domain	PFAM	PF00076	RRM_1	coord: 28..94 e-value: 6.6E-15 score: 54.7
IPR000504	RNA recognition motif domain	PROSITE	PS50102	RRM	coord: 26..101 score: 14.624
IPR012677	Nucleotide-binding alpha-beta plait domain superfamily	GENE3D	G3DSA:3.30.70.330		coord: 21..100 e-value: 3.2E-18 score: 67.6
None	No IPR available	PANTHER	PTHR12785:SF7	SPLICING FACTOR 3B SUBUNIT 6-LIKE PROTEIN	coord: 11..104
None	No IPR available	PANTHER	PTHR12785	splicing factor 3B	coord: 11..104
IPR034150	SF3B6, RNA recognition motif	CDD	cd12241	RRM_SF3B14	coord: 24..99 e-value: 6.44975E-37 score: 119.258
IPR035979	RNA-binding domain superfamily	SUPERFAMILY	SSF54928	RNA-binding domain, RBD	coord: 26..105