SMED30024602

Overview
NameSMED30024602
Smed IDSMED30024602
Length (bp)3396
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30024602 (SMED30024602) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30024602 (SMED30024602) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30024602 (SMED30024602) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30024602 (SMED30024602) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30024602

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30024602SMESG000064764.1 SmedASXL_012772SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30024602SMESG000064764.1 dd_Smed_v4_14271_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
zeta neoblastSMED30024602SMESG000064764.1 dd_Smed_v4_40776_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30024602 vs. Planmine SMEST
Match: SMESG000064764.1 (SMESG000064764.1)

HSP 1 Score: 389.423 bits (999), Expect = 3.843e-123
Identity = 214/232 (92.24%), Postives = 216/232 (93.10%), Query Frame = 2
Query:  104 MADFSELYSNEVKNIVMIMKRAFNIDFNVLNKDHYMEVISVIFSFFLP*QEISNNFEDDKVLFEDLDGNPLKLQHLVNLNESFKNAKDKLSTIFNKSIFNLKVIRPWNIQEYQNYFLRKFITEKEYKSLFLNSICYIDGKFRMRGFAKSDRVNHLKILKIERXXXXXXXXXXXXXXXELLNDPRFKSVYKSVDATYLFPNGEVSVKWQNCFNVFDWPDLLADCDL-VKIFPY 796
            MADFSELYSNEVKNIVMIMKRAFNIDFNVLNKDHYME             EISNNFEDDKVLFEDLDGNPLKLQHLVNLNESFKNAKDKLSTIFNKSIFNLKVIRPWNIQEYQNYFLRKFITEKEYKSLFLNSICYIDGKFRMRGFAKSDRVNHLKILKIERNSKIILKSSNKIKISELLNDPRFKSVYKSVDATYLFPNGEVSVKWQNCFNVFDWPDLLADCDL + I P+
Sbjct:    1 MADFSELYSNEVKNIVMIMKRAFNIDFNVLNKDHYME-------------EISNNFEDDKVLFEDLDGNPLKLQHLVNLNESFKNAKDKLSTIFNKSIFNLKVIRPWNIQEYQNYFLRKFITEKEYKSLFLNSICYIDGKFRMRGFAKSDRVNHLKILKIERNSKIILKSSNKIKISELLNDPRFKSVYKSVDATYLFPNGEVSVKWQNCFNVFDWPDLLADCDLKITIDPF 219          

HSP 2 Score: 254.218 bits (648), Expect = 2.208e-73
Identity = 126/127 (99.21%), Postives = 127/127 (100.00%), Query Frame = 3
Query: 2424 KITIDPFSTNSSLNSILKEIQQLEVIYKAVENDESSWELIDNEKLGLNVDKFKIELEKMLKLCIDKAPCNSDENINIYSWNRNVFTRNKDITEKLWALFHKINSKAEFKKCVNSMMPILTENYASVE 2804
            KITIDPFSTNSSLNSILKEIQQLEVIYKAVENDESSWELID+EKLGLNVDKFKIELEKMLKLCIDKAPCNSDENINIYSWNRNVFTRNKDITEKLWALFHKINSKAEFKKCVNSMMPILTENYASVE
Sbjct:  213 KITIDPFSTNSSLNSILKEIQQLEVIYKAVENDESSWELIDDEKLGLNVDKFKIELEKMLKLCIDKAPCNSDENINIYSWNRNVFTRNKDITEKLWALFHKINSKAEFKKCVNSMMPILTENYASVE 339          
The following BLAST results are available for this feature:
BLAST of SMED30024602 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024602 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024602 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024602 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024602 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024602 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024602 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024602 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024602 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024602 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024602 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024602 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024602 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024602 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000064764.13.843e-12392.24SMESG000064764.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024602 ID=SMED30024602|Name=SMED30024602|organism=Schmidtea mediterranea sexual|type=transcript|length=3396bp
AAGAATATTTAAATAATTATTTTAATTGTAATTGTTTCAGATTTTTAACA
AATACTACAAAATATATTAGACAAATCATTTCTATTCATAAATATATATT
CTAATGGCTGACTTTTCTGAATTATACAGTAATGAGGTGAAAAATATTGT
GATGATAATGAAGCGAGCTTTTAATATTGATTTTAACGTTTTAAATAAAG
ATCATTATATGGAGGTAATTAGTGTCATTTTTTCATTTTTTTTACCATAA
CAGGAAATTTCAAACAACTTTGAAGATGATAAAGTACTATTTGAAGATCT
CGATGGAAATCCATTAAAATTGCAACATTTAGTTAATCTAAATGAAAGTT
TTAAAAATGCCAAGGATAAACTTTCGACTATTTTTAATAAAAGTATTTTT
AATTTAAAAGTTATTAGACCATGGAATATACAAGAATATCAAAATTATTT
TCTCCGTAAGTTTATTACAGAAAAAGAATACAAAAGTTTATTCTTAAACA
GTATTTGTTATATTGATGGTAAATTTCGGATGCGTGGATTTGCAAAATCC
GATAGAGTAAATCATTTAAAAATTCTTAAAATAGAAAGAAATTCTAAAAT
AATATTAAAATCATCAAATAAAATTAAAATATCCGAACTGTTAAATGATC
CCAGATTTAAGAGCGTATATAAAAGTGTGGATGCTACATATTTGTTTCCA
AATGGAGAAGTGTCAGTAAAATGGCAAAATTGTTTCAATGTTTTTGATTG
GCCAGATTTATTGGCGGATTGTGACTTGGTAAAAATCTTTCCCTATATTC
TAGAATAATGATATTATCTCGAAACTTAAAGAAATAAAATAATCTATTAA
ACTGATTACTTCTTGATTTTATTATAAGGAGGATTTGTAAACTCGGAGCA
CAATTGTTAAAATTACAAAATAACTATAAATTATATGAATTACCACAAAA
TAATGTTTTATTGCATTTGTTTAAGAATTTGGTCTTATCCTTTAACGTTG
AATTGTCTGGTTACGATGTTAAATATCGGGAATGAAGTCTGAAGCTTTCC
TCATCATCATTTTTCATTACATCTTTCATCTTGAATAACAAATTCTTAAT
TATACATTCATAGCGTTTCACGGTTTGAGATATGGTTCTACAGTGTTCGG
TTTGGCGTCTGTATGCTCAATTTTTTCAAAATTTTTCATTTTTACGTTGT
ATTTTCAACTGAATTTGTTTTTAACACCTTTCATAAAAGTTTTTTTCCCA
GCCATAATCCTCCTTTTGCTGTTGATCACGATGTGGTTATCGAATTTTTT
GTCATATTATCATTAATGATCTTTTTCTCAACTCCAGTAGCAAACCAATC
AAAGAAGGGTTCCATAGGGAAATGTGAATCCATTAACTGTCGGTTAGCTA
TTGATTATTCCATTTTCTTAGCTTTGAAAATTTCCCAGTGTCTTTATATT
ATTCCATTGGACGAATTCAAACATTCGCAGGTTCATTATTTGTTACACAT
GATCCTGGTTTCATTAGCAATGGAAAAAAATCATTAAACTGGTCTTTCTG
GGTGTGCTTTATCCTTCACATTTTTGAAATTTCGATTATACACCGTGTTG
ATTTCCTTTTGGTTTATTCGTAATCTCGGTAGGAACATGAGTTAGAAACG
GGATAAATTTGATTGAAATTTATTTGATTGAATGAAATGCGCATCAGTTT
GTGGACTGATATGATACTTTGCATTTTGAAATCAATTGCTGTAAGGGTAA
CTATTGTTGATATTAAAATTGAACAATAGTGCACCTTACGTGTTGTGATT
TATTGGTAAAATGCACTTTAAAGTTACAAGTACTATTGTATAAACAATCA
GTTAACTCTCATGAACAAGGTTTCAATTTGTGTTCAAAAGTACCTTAAAA
TTATTACTATAAGCCTCATGGCATATATTTCGGGGGGGGAATCAATTTTA
CCAGCCAAAGCTGTGAACTATGGTTCTGTTTTAAATTATGAGGAATAATC
ACATCACTGTTATTGATAACAAAAAGTGTTTTATTCAAATATCTTCTAAT
CACTTGAATGACACTACTTATAATGAAAAATTCCGAATGTCTATTCAAAA
GCTATCGTTGTTACATCCTGCGCCAACAGAAAAAAGAATTACCATTTCTA
CTTTTCACCATATGCGTTGGAGGCACCATTAGGATTTTACACCAAAGAAT
TAAAGAATGTCTTGATCATAGTAAAAAAATCACAGGTAATAAGATATCTT
TAACATTATCAGCCAATACCATTTAAAATTATTTCTATCTTTCATTTAGT
TTATATTTTTCACTGAGCAAAATTTTTATTTCTAATTGGAACTTTATCTA
AAATCTAAATTTGTTACACTGTTCAATTTCAGTTCAATAATGTAATGATA
AACTATTTAAAGTATTTTTTTAGAAAATAACCATTGATCCATTTTCTACA
AATAGTAGTCTTAATTCAATTCTTAAAGAAATACAGCAACTAGAAGTCAT
TTATAAAGCAGTAGAAAACGACGAGAGTTCTTGGGAATTAATTGATAATG
AGAAACTTGGATTAAATGTTGATAAATTTAAAATTGAATTGGAAAAAATG
CTGAAACTGTGTATCGATAAAGCTCCATGCAACTCTGATGAGAATATTAA
CATCTATTCATGGAATCGAAATGTATTTACAAGAAATAAAGATATCACTG
AGAAACTGTGGGCGCTTTTTCACAAAATCAATAGCAAAGCTGAATTTAAA
AAGTGTGTTAATTCAATGATGCCAATTCTTACTGAAAATTATGCCAGTGT
TGAGGTGAGCTCGATCCGATAACATTTTAACATCCTTTTAATAAATGAAT
CACTGTTATTGGGTGGGGGCAATTTGCACACTTGGAAATCAATCTTTTAT
ACTAATAAACTGTGACAGGATAAAATACGCGACTAAGAAATATATTGGTG
ACAATTGTTCAACAAACAATCAAAATATCAATCGATATAATATAATCTAG
CTTTTTCGCATTTACTTGCAGGTCTATAACAAAAATTCTACCCACATACT
TTGACACTGAAAGTTATTTTGAGGTCTCCTTGGCAGTTGATCAATGTAAA
GAATATGTGCTAGTTTAAGACATAAAATACAAATTGTATTTTTTTCCTTT
TTAAACCAAGAAAATATTACATTTTATATTTATAATAGGATTTTCAGATT
TGGTAAATTTGCCAATGAAAACTGTAATTTTTATTAAAATAAAATTAGGA
AAATGATTGCATTCGTTGAAACAAAAATGTTATTATAAACTTTTTTGTTT
ATTTATACTATTGATAAAAATTAAGTAGTTTATTTGATAGATCAAAATAT
TAAAAATTTTGGTAAATAAACTTTATTTTAATAAGCCTAAACCTTT
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protein sequence of SMED30024602-orf-1

>SMED30024602-orf-1 ID=SMED30024602-orf-1|Name=SMED30024602-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=125bp
MLNFFKIFHFYVVFSTEFVFNTFHKSFFPSHNPPFAVDHDVVIEFFVILS
LMIFFSTPVANQSKKGSIGKCESINCRLAIDYSIFLALKISQCLYIIPLD
EFKHSQVHYLLHMILVSLAMEKNH*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000429neoblast
PLANA:0002109X1 cell