SMED30024572
|
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30024572 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Homology
BLAST of SMED30024572 vs. Ensembl Zebrafish
Match: BX511082.1 (pep chromosome:GRCz11:9:14291932:14297132:1 gene:ENSDARG00000113678.1 transcript:ENSDART00000183119.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX511082.1) HSP 1 Score: 75.8702 bits (185), Expect = 1.032e-26 Identity = 52/146 (35.62%), Postives = 76/146 (52.05%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*AL---IFKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTIKVYIQR*KIELVKA------PKCVSRLRSFLG 2109
DL AY+ V + E + KTAF+T F + LPF LS APA FQ L + L + + + VYLD+ILI + + +EH + + V + ++GL + EK F+ F+GH + V R E V+A P L+ FLG
Sbjct: 614 DLRNAYHLVRIREGDEWKTAFNTPRGHFEYCVLPFGLSNAPAVFQALVNDVLRDMLDQFIYVYLDDILIFSHSLQEHVQHVRRVLQRLLENGLYVKAEKCVFHAQSVPFLGHIVSVEGMRMDPEKVQAVVDWPTPDSRKALQRFLG 759 HSP 2 Score: 47.3654 bits (111), Expect = 1.032e-26 Identity = 21/58 (36.21%), Postives = 36/58 (62.07%), Query Frame = -3
Query: 2156 ISKKLTESKNYQKVSKCRSPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMPDM 2329
+ K +++S + + SP + V +KDGS+R C+D+R LNA+T K ++P+P M
Sbjct: 540 MEKYISDSLAAKIIRPSSSPAGAGFFFVKKKDGSLRPCIDYRGLNAITVKNTYPLPLM 597 HSP 3 Score: 31.9574 bits (71), Expect = 1.032e-26 Identity = 16/45 (35.56%), Postives = 23/45 (51.11%), Query Frame = -2
Query: 1494 WNEECKNSFNSLKKVLCEAPVLEFPMSDRMFIIYTDAS---FGAI 1619
W+ + +F+ LK AP+L P R F++ DAS GAI
Sbjct: 790 WSNAAQVAFDRLKSCFVSAPILIAPDPSRQFVVEVDASEVGVGAI 834 HSP 4 Score: 26.5646 bits (57), Expect = 1.032e-26 Identity = 8/15 (53.33%), Postives = 12/15 (80.00%), Query Frame = -3
Query: 1661 FGLTNYYRRFIKDYS 1705
G N+YRRFI+++S
Sbjct: 758 LGFANFYRRFIRNFS 772 HSP 5 Score: 69.707 bits (169), Expect = 3.221e-12 Identity = 50/187 (26.74%), Postives = 82/187 (43.85%), Query Frame = -3
Query: 395 VLVPQNYVTTLINYIHIE--LCHLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTRELLLPR--PGEKFSEQMVIDIAYMETSEWNKKYVIVIIDRFSK---LVSLTGTTEQDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAERT 934
+ VP+ + +I + H CH G+ +TLY +K F+W +++ + + CS+CA K LL P P +S + + + S N ++ ++DRFSK + L E V+D+ G P +++DRG F RE+ G +S + QSNG ER
Sbjct: 992 LFVPEELRSDVIRWGHSSKLACHPGVSRTLYLIKQRFWWPVMARDIRNFVLACSVCAVSKTSNRPPAGLLQPLSVPSRPWSHIALDFVTGLPPSNGN-TVILTVVDRFSKATHFIPLPKLPSARETAA--AVIDHVFRIHGLPTDVVSDRGPQFISKFWREFCHLMGATVSLSSGFHPQSNGQTERA 1175 HSP 6 Score: 23.0978 bits (48), Expect = 3.221e-12 Identity = 25/126 (19.84%), Postives = 56/126 (44.44%), Query Frame = -2
Query: 6 NNWCELLPRIEFCLNAKIQSSTGFSTFD--IVYS--------RKINLISNSGNVQELKDEILKSAKVNIENAGNSMKGSDQDKRGTRILDVGEKVLVRLK--PHKRKKNGML--YEGPYEILRFIS 341
++W + L +E+ N+ S+TG S F+ + Y ++ + S VQ + ++ + ++ + +D+ + + VG+KV + K P + N + + GP+ + + IS
Sbjct: 1192 SSWSQQLSWVEYAHNSLPVSATGLSPFECSLGYQPPAFPSLESEVAVPSAHAFVQRCRRTWNRARQTLLQVGLRTKAKADRHRSKPPVYVVGQKVWLSSKNIPLRTVCNKLAPKFIGPFSVTKIIS 1317
BLAST of SMED30024572 vs. Ensembl Zebrafish
Match: BX546500.1 (pep chromosome:GRCz11:23:12926092:12931693:-1 gene:ENSDARG00000086495.3 transcript:ENSDART00000122176.3 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX546500.1) HSP 1 Score: 74.3294 bits (181), Expect = 9.917e-26 Identity = 51/146 (34.93%), Postives = 75/146 (51.37%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*AL---IFKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTIKVYIQR*KIELVKA------PKCVSRLRSFLG 2109
DL AY+ V + + KTAF+T F + LPF LS APA FQ L + L I + + VYLD+ILI + + +EH + + V + ++GL + EK F+ F+GH + V R E ++A P L+ FLG
Sbjct: 588 DLRNAYHLVRIRPGDEWKTAFNTPRGHFEYCVLPFGLSNAPAVFQALVNDVLRDMIDQFIYVYLDDILIFSHSLQEHVQHVRRVLQRLLENGLYVKAEKCVFHAQSVQFLGHIVSVEGMRMDPEKIQAVVDWPTPDSRKALQRFLG 733 HSP 2 Score: 45.8246 bits (107), Expect = 9.917e-26 Identity = 20/58 (34.48%), Postives = 36/58 (62.07%), Query Frame = -3
Query: 2156 ISKKLTESKNYQKVSKCRSPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMPDM 2329
+ K +++S + + SP + V +KDGS+R C+D+R LN++T K ++P+P M
Sbjct: 514 MEKYISDSLAAKIIRPSSSPAGAGFFFVKKKDGSLRPCIDYRGLNSITVKNTYPLPLM 571 HSP 3 Score: 31.5722 bits (70), Expect = 9.917e-26 Identity = 16/45 (35.56%), Postives = 23/45 (51.11%), Query Frame = -2
Query: 1494 WNEECKNSFNSLKKVLCEAPVLEFPMSDRMFIIYTDAS---FGAI 1619
W+ + +F+ LK AP+L P R F++ DAS GAI
Sbjct: 764 WSSAAEAAFSKLKGCFVSAPILIAPDPSRQFVVEVDASEVGVGAI 808 HSP 4 Score: 26.5646 bits (57), Expect = 9.917e-26 Identity = 8/15 (53.33%), Postives = 12/15 (80.00%), Query Frame = -3
Query: 1661 FGLTNYYRRFIKDYS 1705
G N+YRRFI+++S
Sbjct: 732 LGFANFYRRFIRNFS 746 HSP 5 Score: 73.9442 bits (180), Expect = 2.517e-13 Identity = 52/187 (27.81%), Postives = 85/187 (45.45%), Query Frame = -3
Query: 395 VLVPQNYVTTLINYIHIE--LCHLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTRELLLPR--PGEKFSEQMVIDIAYMETSEWNKKYVIVIIDRFSK---LVSLTGTTEQDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAERT 934
+ VP+ + ++ + H CH G+ +TL+ +K F+W +++ V + CS+CA K LL P P +S + + + +S N V+ ++DRFSK +SL E V V+D+ G P +++DRG F RE+ G +S + QSNG ER
Sbjct: 966 LFVPEELRSDVVRWGHSSKVACHPGVSRTLFVIKQRFWWPTMARDVRDFVLACSVCAVSKSSNRPPAGLLQPLSVPSRPWSHISLDFVTGLPSSNGN-TVVLTVVDRFSKAAHFISLPKLPSARETAV--AVIDHVFRIHGLPTDVVSDRGPQFVSRFWREFCRLLGATVSLSSGFHPQSNGQTERA 1149 HSP 6 Score: 23.0978 bits (48), Expect = 2.517e-13 Identity = 10/28 (35.71%), Postives = 16/28 (57.14%), Query Frame = -2
Query: 258 NNWCELLPRIEFCLNAKIQSSTGFSTFD 341
++W + L +E+ N+ SSTG S F
Sbjct: 1166 SSWSQQLSWVEYAHNSLPVSSTGLSPFQ 1193
BLAST of SMED30024572 vs. Ensembl Zebrafish
Match: CR925755.2 (pep chromosome:GRCz11:17:42486740:42492668:-1 gene:ENSDARG00000116402.1 transcript:ENSDART00000183946.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR925755.2) HSP 1 Score: 76.6406 bits (187), Expect = 1.541e-25 Identity = 52/146 (35.62%), Postives = 76/146 (52.05%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*AL---IFKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTIKVYIQR*KIELVKA------PKCVSRLRSFLG 2109
DL AY+ V + + KTAF+T F + LPF LS APA FQ L + L I + + VYLD+ILI + + +EH + + V + ++GL + EK F+ F+GHT+ V R E ++A P L+ FLG
Sbjct: 587 DLRNAYHLVRIRPGDEWKTAFNTPRGHFEYCVLPFGLSNAPAVFQALVNDVLRDMIDQFIYVYLDDILIFSHSLQEHVQHVRRVLQRLLENGLYVKAEKCVFHAQSVQFLGHTVSVEGMRMDPEKIQAVVNWPTPDSRKALQRFLG 732 HSP 2 Score: 45.8246 bits (107), Expect = 1.541e-25 Identity = 20/58 (34.48%), Postives = 36/58 (62.07%), Query Frame = -3
Query: 2156 ISKKLTESKNYQKVSKCRSPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMPDM 2329
+ K +++S + + SP + V +KDGS+R C+D+R LN++T K ++P+P M
Sbjct: 513 MEKYISDSLAAKIIRPSSSPAGAGFFFVKKKDGSLRPCIDYRGLNSITVKNTYPLPLM 570 HSP 3 Score: 29.6462 bits (65), Expect = 1.541e-25 Identity = 15/45 (33.33%), Postives = 22/45 (48.89%), Query Frame = -2
Query: 1494 WNEECKNSFNSLKKVLCEAPVLEFPMSDRMFIIYTDAS---FGAI 1619
W+ + +F+ LK AP+L P R F++ D S GAI
Sbjct: 763 WSNAAEAAFSKLKGCFVSAPILIAPDPSRQFVVEVDVSEVGVGAI 807 HSP 4 Score: 25.0238 bits (53), Expect = 1.541e-25 Identity = 7/14 (50.00%), Postives = 11/14 (78.57%), Query Frame = -3
Query: 1664 FGLTNYYRRFIKDY 1705
G N+YRRFI+++
Sbjct: 731 LGFANFYRRFIRNF 744 HSP 5 Score: 72.4034 bits (176), Expect = 1.118e-12 Identity = 53/187 (28.34%), Postives = 85/187 (45.45%), Query Frame = -3
Query: 395 VLVPQNYVTTLINYIHIE--LCHLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTRELLLPR--PGEKFSEQMVIDIAYMETSEWNKKYVIVIIDRFSK---LVSLTGTTEQDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAERT 934
+ VP++ + +I + H CH G+ +TL+ +K F+W +++ V + CS+CA K LL P P +S + + + +S N V+ ++DRFSK V L E V V+D+ G P +++DRG F RE+ G +S + QSNG ER
Sbjct: 965 LFVPEDLRSDVIWWGHSSKVACHPGVSRTLFVIKQRFWWPVMARDVRDFVLACSVCAASKSSNRPPAGLLQPLSVPSRPWSHISLDFVTGLPSSNGN-TVVLTVVDRFSKAAHFVPLPKLPSARETAV--AVIDHVFRIHGLPTDVVSDRGPQFVSKFWREFCRLLGATVSLSSGFHPQSNGQTERA 1148 HSP 6 Score: 21.9422 bits (45), Expect = 1.118e-12 Identity = 9/28 (32.14%), Postives = 16/28 (57.14%), Query Frame = -2
Query: 258 NNWCELLPRIEFCLNAKIQSSTGFSTFD 341
++W + L +E+ N+ S+TG S F
Sbjct: 1165 SSWSQQLSWVEYAHNSLPVSATGLSPFQ 1192
BLAST of SMED30024572 vs. Ensembl Zebrafish
Match: BX511224.1 (pep chromosome:GRCz11:2:18017000:18022765:1 gene:ENSDARG00000113243.1 transcript:ENSDART00000186877.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX511224.1) HSP 1 Score: 73.1738 bits (178), Expect = 3.609e-25 Identity = 51/146 (34.93%), Postives = 75/146 (51.37%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*AL---IFKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTIKVYIQR*KIELVKA------PKCVSRLRSFLG 2109
DL AY+ V + + KTAF+T F + LPF LS APA FQ L + L I + + VYLD+ILI + + +EH + + V + ++GL + EK F+ F+GH + V R E ++A P L+ FLG
Sbjct: 614 DLRNAYHLVCIRPGDEWKTAFNTPRGHFEYCVLPFGLSNAPAVFQALVNDVLRDMIDQFIYVYLDDILIFSHSLQEHIQHVRRVLQRLLENGLYVKAEKCVFHAQSVQFLGHIVSVEGMRMDPEKIQAVVNWPTPDSRKALQRFLG 759 HSP 2 Score: 45.8246 bits (107), Expect = 3.609e-25 Identity = 20/58 (34.48%), Postives = 36/58 (62.07%), Query Frame = -3
Query: 2156 ISKKLTESKNYQKVSKCRSPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMPDM 2329
+ K +++S + + SP + V +KDGS+R C+D+R LN++T K ++P+P M
Sbjct: 540 MEKYISDSLAAKIIRPSSSPAGAGFFFVKKKDGSLRPCIDYRGLNSITVKNTYPLPLM 597 HSP 3 Score: 31.9574 bits (71), Expect = 3.609e-25 Identity = 16/45 (35.56%), Postives = 23/45 (51.11%), Query Frame = -2
Query: 1494 WNEECKNSFNSLKKVLCEAPVLEFPMSDRMFIIYTDAS---FGAI 1619
W+ + +F+ LK AP+L P R F++ DAS GAI
Sbjct: 790 WSSAAEAAFSKLKGCFVSAPILIAPDPSRQFVVEVDASEVGVGAI 834 HSP 4 Score: 25.409 bits (54), Expect = 3.609e-25 Identity = 8/15 (53.33%), Postives = 11/15 (73.33%), Query Frame = -3
Query: 1661 FGLTNYYRRFIKDYS 1705
G N+YRRFI ++S
Sbjct: 758 LGFANFYRRFIHNFS 772 HSP 5 Score: 73.1738 bits (178), Expect = 2.335e-13 Identity = 51/187 (27.27%), Postives = 85/187 (45.45%), Query Frame = -3
Query: 395 VLVPQNYVTTLINYIHIE--LCHLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTRELLLPR--PGEKFSEQMVIDIAYMETSEWNKKYVIVIIDRFSK---LVSLTGTTEQDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAERT 934
+ VP+ + ++ + H CH G+ +TL+ +K F+W +++ V + CS+CA K LL P P +S + + + +S N ++ ++DRFSK +SL E V V+D+ G P +++DRG F RE+ G +S + QSNG ER
Sbjct: 992 LFVPEELRSDVVRWGHSSKVACHPGVSRTLFVVKQRFWWPAMARDVRDFVLACSVCAVSKSSNRPPAGLLQPLSVPSRPWSHISLDFVTGLPSSNGN-TVILTVVDRFSKAAHFISLPKLPSARETAV--AVIDHVFRIHGLPTDVVSDRGPQFVSKFWREFCRLLGATVSLSSGFHPQSNGQTERA 1175 HSP 6 Score: 23.8682 bits (50), Expect = 2.335e-13 Identity = 28/127 (22.05%), Postives = 54/127 (42.52%), Query Frame = -2
Query: 6 NNWCELLPRIEFCLNAKIQSSTGFSTFDIVYSRK----------INLISNSGNVQELKDEILKSAKVNIENAGNSMKG-SDQDKRGTRILDVGEKVLVRLK--PHKRKKNGML--YEGPYEILRFIS 341
++W + L +E+ N+ S+TG S+F + + + S VQ + A+ + AG K +D+ + + VG+KV + K P + N + + GP+ + + IS
Sbjct: 1192 SSWSQQLSWVEYAHNSLPVSATGLSSFQCSLGYQPPVFPSLDSEVAVPSVHAFVQRCR-RTWNRARQTLLQAGTRTKAKADRHRSKPPVYVVGQKVWLSSKNIPLRTVCNKLAPKFIGPFIVTKIIS 1317
BLAST of SMED30024572 vs. Ensembl Zebrafish
Match: CR855320.1 (pep chromosome:GRCz11:1:7956030:7961696:1 gene:ENSDARG00000099359.2 transcript:ENSDART00000159655.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CR855320.1) HSP 1 Score: 72.0182 bits (175), Expect = 6.034e-25 Identity = 51/146 (34.93%), Postives = 74/146 (50.68%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*AL---IFKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTIKVYIQR*KIELVKA------PKCVSRLRSFLG 2109
DL AY+ V + + K+AF+T F + LPF LS APA FQ + L I + + VYLD+ILI + + +EH + I V + ++GL + EK F+ F+GH + V R E +KA P L+ FLG
Sbjct: 616 DLRNAYHLVRIRAGDEWKSAFNTPRGHFEYCVLPFGLSNAPAVFQAFVNDVLRDMIDQFIYVYLDDILIFSHSLQEHVQHIRRVLQRLLENGLYVKAEKCVFHAQSVPFLGHIVSVEGLRMDPEKIKAVVNWPTPDSRKALQRFLG 761 HSP 2 Score: 45.0542 bits (105), Expect = 6.034e-25 Identity = 20/58 (34.48%), Postives = 35/58 (60.34%), Query Frame = -3
Query: 2156 ISKKLTESKNYQKVSKCRSPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMPDM 2329
+ K +++S + + SP + V +KDGS+R C+D+R LN +T K ++P+P M
Sbjct: 542 MGKYISDSLAAKIIRPSSSPAGAGFFFVKKKDGSLRPCIDYRGLNNITVKNTYPLPLM 599 HSP 3 Score: 31.9574 bits (71), Expect = 6.034e-25 Identity = 16/45 (35.56%), Postives = 23/45 (51.11%), Query Frame = -2
Query: 1494 WNEECKNSFNSLKKVLCEAPVLEFPMSDRMFIIYTDAS---FGAI 1619
W+ + +F+ LK AP+L P R F++ DAS GAI
Sbjct: 792 WSSAAEAAFSKLKGCFVSAPILITPDPSRQFVVEVDASEVGVGAI 836 HSP 4 Score: 26.5646 bits (57), Expect = 6.034e-25 Identity = 8/15 (53.33%), Postives = 12/15 (80.00%), Query Frame = -3
Query: 1661 FGLTNYYRRFIKDYS 1705
G N+YRRFI+++S
Sbjct: 760 LGFANFYRRFIRNFS 774 HSP 5 Score: 71.2478 bits (173), Expect = 1.913e-12 Identity = 49/185 (26.49%), Postives = 81/185 (43.78%), Query Frame = -3
Query: 395 VLVPQNYVTTLINYIHIE--LCHLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTRELLLPR--PGEKFSEQMVIDIAYMETSEWNKKYVIVIIDRFSKLVSLTGTTEQDEITVFNVVLDNWIYRF-GKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAERT 934
+ VP+ + +I + H CH G+ +T + +K F+W L++ V + CS+CA K LL P P +S + + + S N ++ ++DRFSK + V + N ++R G P +++DRG F RE+ G +S + QSNG ER
Sbjct: 994 LFVPEKLRSDVIRWGHSSKVACHPGVSRTSFVIKQRFWWPALARDVRDFVLACSVCAVSKTSNRPPAGLLQPLSVPSRPWSHISLDFVTGLPPSNGN-TVILTVVDRFSKAAHFVPLPKLPSARETAVAVINHVFRIHGLPTDVVSDRGPQFISKFWREFCRLLGATVSLSSGFHPQSNGQTERA 1177 HSP 6 Score: 22.7126 bits (47), Expect = 1.913e-12 Identity = 25/126 (19.84%), Postives = 55/126 (43.65%), Query Frame = -2
Query: 6 NNWCELLPRIEFCLNAKIQSSTGFSTFD--IVYS--------RKINLISNSGNVQELKDEILKSAKVNIENAGNSMKGSDQDKRGTRILDVGEKVLVRLK--PHKRKKNGML--YEGPYEILRFIS 341
++W + L +E+ N+ S+TG S F + Y ++ + S VQ + ++ + ++ + +D+ + + VG+KV + K P + N + + GP+ + + IS
Sbjct: 1194 SSWSQQLSWVEYAHNSLPVSATGLSPFQCSLGYQPPVFPSLESEVAVPSVHAFVQRCRRTWSRARQTLLQVGVRTKAKADRHRSKPPVYVVGQKVWLSTKNIPLRTVCNKLAPKFIGPFIVTKIIS 1319
BLAST of SMED30024572 vs. Ensembl Xenopus
Match: ENSXETT00000006041.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:6:37317867:37322724:1 gene:ENSXETG00000003070.1 transcript:ENSXETT00000006041.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 98.2117 bits (243), Expect = 1.193e-56 Identity = 58/146 (39.73%), Postives = 87/146 (59.59%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRL---TH*ALIFKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTIK---VYIQR*KIELVKA---PKCVSRLRSFLG 2109
DL YYQ+ + E + KTAF F FNR+P L APATFQRL T + V+VYLD++++ +T EEH++ + +VF ++K+GLK++ EK QF + N++GH + + KIE V + P+ ++ LRSFLG
Sbjct: 416 DLRSGYYQLPMHPEDKEKTAFICPLGFFEFNRMPQGLCGAPATFQRLMERTVGDMHLLEVLVYLDDLIVFGRTLEEHEQRLLKVFDRLEKEGLKLSPEKCQFCQPSVNYVGHVVSAEGIATDPSKIEAVSSWPKPRTITELRSFLG 561 HSP 2 Score: 84.3445 bits (207), Expect = 1.193e-56 Identity = 87/412 (21.12%), Postives = 169/412 (41.02%), Query Frame = -3
Query: 398 IVYGSRHLTAREMGYYSQEKNYYSIMNLLC--ILENLYGNKFIVRTDHKALTFMNTTK--NQLAHNFRRSSQTYQ-NMILSCSIENEMIMEMQMDFHG*KMSFAHNAKQSIKMQRRKIKSEVYKQFTRKCK------------------YCGVS----------------KNQNEDIIFKEIIPLLKGEKETVYDEVKQCKYFILIEQLCIL------------SIIEDNKILVLVPQNYVTTLINYIHIELCHLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTRELLLPRPGEKFSEQMVIDIAYMETSEWNKKYVIVIIDRFSKLVSLTGTTEQDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAER 1480
+ + SR L+ E Y + + + ++ + + E LYG +F V+TD+ LT++ TT + H + + Y+ ++ +N + HG H + I++ +++ + + T +C+ YC V+ ++Q+ED + + + L+ ++ + +L+++ L S + K +++PQ + +++ +H E HLG KTL ++D FYW + Q V C C +RK R L + + ID +E E V+V+ D +++ T +Q ITV V+++ + +G P I +D+G+ FE E M G+ + +PY Q + ER
Sbjct: 672 VAFISRSLSPSERNYPAHKLEFLALKWAVVDKLHEYLYGAEFEVQTDNNPLTYILTTAKLDATGHRWLAALAHYKFSLRYRPGRDNRDADGLSRRPHG----DLHPDDEWIEIPAPGVRT-MCQGITGRCQSGNFAEKIGMTVRGIPKLYCNVTSVGTSTMPALNKGDIKRDQSEDPLCQMAMEALRKKQVQILKTDSHPLASLLVKEWDRLRLRDGLVYRRAPSATDSEKWQLMLPQKHRDSVLMALHDEHGHLGYDKTLGLVRDRFYWPCMKQDVEDYCRSCLRCIQRKTLPSRAAPLSHMESHSPL-DLVCIDFLSIEPDEGGTSNVLVVTDHYTRYAQAFPTKDQRAITVAKVLVERFFIHYGLPKRIHSDQGRDFESKLVHELMSMLGVLKSRTTPYHPQGDPQPER 1077 HSP 3 Score: 49.2914 bits (116), Expect = 1.193e-56 Identity = 24/56 (42.86%), Postives = 37/56 (66.07%), Query Frame = -3
Query: 2168 ISKKLTESKNYQKVSKCRSPKNSPLVIVGQKDGSIRICLDFRRLN--AVTDKYSFP 2329
+ K L E K + + RSP SP+V+V +K+GSIR+C+D+R LN + D+Y+ P
Sbjct: 342 LRKHLEELKAAGIIKESRSPYASPIVVVRKKNGSIRMCVDYRTLNQRTIPDQYTTP 397 HSP 4 Score: 38.891 bits (89), Expect = 1.193e-56 Identity = 16/45 (35.56%), Postives = 26/45 (57.78%), Query Frame = -2
Query: 1506 RNNKQIIWNEECKNSFNSLKKVLCEAPVLEFPMSDRMFIIYTDAS 1640
R + + W EEC +F+ LK L APVL + + + + ++ DAS
Sbjct: 609 RESIEDFWTEECDKAFDQLKYCLTHAPVLAYADATKPYTLHIDAS 653 HSP 5 Score: 27.7202 bits (60), Expect = 1.193e-56 Identity = 23/108 (21.30%), Postives = 48/108 (44.44%), Query Frame = -2
Query: 78 KENNWCELLPRIEFCLNAKIQSSTGFSTFDIVYSRKINLI---------------SNSGNVQELKDEILKSAKVNIENAG-NSMKGSDQDKRGTRILDV--GEKVLVR 347
K+ +W + + N+ +TG+S + +++ R+ L S+S V LK ++ ++ K E G +M+ + +I D+ G++VL+R
Sbjct: 1093 KKTHWSRHIATVVHAYNSTKNGATGYSPYFLMFGREARLPIDTVFGVTADDTPVKSHSSYVDRLKRDLQRAYKRAQEAVGKKNMQNKTLYDKKVKIHDLQPGDRVLLR 1200 HSP 6 Score: 24.2534 bits (51), Expect = 1.193e-56 Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = -3
Query: 1661 FGLTNYYRRFIKDYS 1705
G YYRRF+K +S
Sbjct: 560 LGFCGYYRRFVKGFS 574 HSP 7 Score: 21.1718 bits (43), Expect = 1.193e-56 Identity = 12/49 (24.49%), Postives = 24/49 (48.98%), Query Frame = -2
Query: 2367 ILNFEKKSFFGQIISEFRNIFMASRFDLGYTKIIQHEIKTVGSPIFRTR 2513
++ E KS + ++E +F + D+G + +H+I+ FR R
Sbjct: 282 VIPPEWKSRLRKHLNEHSALFSRNDLDMGCSTSTKHKIRLREDKPFRER 330
BLAST of SMED30024572 vs. Ensembl Xenopus
Match: anxa6 (annexin A6 [Source:Xenbase;Acc:XB-GENE-989741]) HSP 1 Score: 74.3294 bits (181), Expect = 1.981e-30 Identity = 48/147 (32.65%), Postives = 80/147 (54.42%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*ALIFKGV----VVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTI---KVYIQR*KIELV---KAPKCVSRLRSFLG 2109
DL + Y+Q+ L+ +Q K+AF T + F +PF + APATFQRL + + +G+ YLD+I + +QT EEH + + VF+ I+ GL + EK +E ++GH + ++ K+E + PK ++ +FLG
Sbjct: 427 DLSRGYWQIPLAPSAQEKSAFLTPFGLYQFTVMPFGMRNAPATFQRLVN--RLLEGMQDFAQAYLDDIAVFSQTWEEHLQHLQRVFAQIQDAGLTLKPEKCHLAMAEVQYLGHRVGGGQLRPDPAKVEAICQWPIPKTQKQVLAFLG 571 HSP 2 Score: 53.5286 bits (127), Expect = 1.981e-30 Identity = 23/56 (41.07%), Postives = 36/56 (64.29%), Query Frame = -3
Query: 2162 ISKKLTESKNYQKVSKCRSPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMP 2329
+ ++ E + ++ SP SP+V+V +KDGS R C+D+RRLN VT ++PMP
Sbjct: 353 MKSQIDEMLAFGVITPSHSPWASPVVLVPKKDGSTRFCVDYRRLNDVTTTDAYPMP 408 HSP 3 Score: 47.7506 bits (112), Expect = 1.981e-30 Identity = 21/48 (43.75%), Postives = 32/48 (66.67%), Query Frame = -2
Query: 1500 RNNKQIIWNEECKNSFNSLKKVLCEAPVLEFPMSDRMFIIYTDAS-FG 1640
+ ++ I+W EC+++ N+LK+ L +PVL P R FI+ TDAS FG
Sbjct: 597 QRSRTIVWTPECESAMNALKQALASSPVLAAPDFSRRFILQTDASNFG 644 HSP 4 Score: 41.2022 bits (95), Expect = 7.963e-7 Identity = 37/140 (26.43%), Postives = 64/140 (45.71%), Query Frame = -2
Query: 21 MITTSLKGGFKENNWCELLPRIEFCLNAKIQSSTGFSTFDIVYSRKI----NLI----SNSGNVQELK------------DEILKSAKVNIENAGNSMK-GSDQDKRGTRILDVGEKVLVRLKPHKRKKNGMLYEGPYEI 377
M+ T ++ G E +W LP + F Q STGFS F+++Y R++ +L+ + QE+ +++ A ++ A K D+ R R ++ G+KVL+ L P + K +EGPY +
Sbjct: 86 MLRTFVESG--EKDWERYLPHLLFAYREVPQESTGFSPFELLYGRRVRGPLDLLCEYWEGAPQSQEVPIIPYVLKFRQRLEQMTSLAHDHLSAAQQRQKVWYDRKARERRFME-GDKVLL-LVPTRHDKLQAAWEGPYVV 221 HSP 5 Score: 33.8834 bits (76), Expect = 7.963e-7 Identity = 19/48 (39.58%), Postives = 26/48 (54.17%), Query Frame = -3
Query: 398 RFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAER 541
R G P IL+D+G F + + G+R +SPY Q+NGL ER
Sbjct: 31 RVGFPSEILSDQGPQFTSQLLQCLWQRCGVRAIHSSPYHPQTNGLCER 78
BLAST of SMED30024572 vs. Ensembl Xenopus
Match: alkbh5 (alkB homolog 5, RNA demethylase [Source:Xenbase;Acc:XB-GENE-987580]) HSP 1 Score: 78.1814 bits (191), Expect = 3.966e-22 Identity = 55/145 (37.93%), Postives = 78/145 (53.79%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*AL-IFKG-VVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTIK---VYIQR*KIELVKA---PKCVSRLRSFLG 2109
DL + Y+QV L+E ++ KTAFST E Q+ + RLPF L APATFQR+ L +G YLD+++I + E H + V I K GL N +K E ++G+ I V Q KIE ++ P ++R+FLG
Sbjct: 325 DLTKGYWQVPLTEGAKEKTAFSTPEGQWQYKRLPFGLHGAPATFQRMMDRILKPHRGYASAYLDDVVIFSPDWESHLPRVQAVLDSIWKAGLTANPKKCAMGLEEARYLGYVIGRGVVKPQVSKIEGIQQWPRPNSKKQVRAFLG 469 HSP 2 Score: 45.8246 bits (107), Expect = 3.966e-22 Identity = 20/43 (46.51%), Postives = 30/43 (69.77%), Query Frame = -3
Query: 2162 VSKCRSPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMP 2290
+ + S +SP+V+V + DGSIR C DFR+LN V+ ++PMP
Sbjct: 264 IEESHSEWSSPIVLVPKPDGSIRFCNDFRKLNEVSKFDAYPMP 306 HSP 3 Score: 22.7126 bits (47), Expect = 3.966e-22 Identity = 6/16 (37.50%), Postives = 11/16 (68.75%), Query Frame = -3
Query: 1661 IFGLTNYYRRFIKDYS 1708
G+ YYRRF+ +++
Sbjct: 467 FLGIVGYYRRFVPNFA 482
BLAST of SMED30024572 vs. Ensembl Xenopus
Match: ENSXETT00000034712.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:9:33567219:33572531:-1 gene:ENSXETG00000011772.1 transcript:ENSXETT00000034712.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 65.4698 bits (158), Expect = 5.189e-21 Identity = 42/114 (36.84%), Postives = 63/114 (55.26%), Query Frame = -1
Query: 1777 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*A---LIFKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTI 2109
DL AY + + E + KTAF+TR+ + + +PF L APA FQ + L+ K VVVYLD+ILI +Q E H + E S ++++ L EK F + +F+G+ I
Sbjct: 610 DLRGAYNLIRIREGDEWKTAFNTRDGHYEYLVMPFGLCNAPAVFQEFVNDIFRDLLGKSVVVYLDDILIFSQDLETHRSQVKEALSRLRENFLFAKLEKCTFEVPKISFLGYII 723 HSP 2 Score: 41.2022 bits (95), Expect = 5.189e-21 Identity = 19/51 (37.25%), Postives = 30/51 (58.82%), Query Frame = -3
Query: 2162 SKNYQK--VSKCRSPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMP 2308
S+N Q+ + SP + V +KDG +R C+D+R LN +T K +P+P
Sbjct: 541 SENLQRGFIRPSTSPAGAGFFFVEKKDGGLRPCIDYRGLNKITVKNRYPLP 591 HSP 3 Score: 28.8758 bits (63), Expect = 5.189e-21 Identity = 10/14 (71.43%), Postives = 12/14 (85.71%), Query Frame = -3
Query: 1661 GLTNYYRRFIKDYS 1702
G NYYR+FIKD+S
Sbjct: 755 GFANYYRQFIKDFS 768 HSP 4 Score: 26.9498 bits (58), Expect = 5.189e-21 Identity = 14/38 (36.84%), Postives = 15/38 (39.47%), Query Frame = -2
Query: 1506 WNEECKNSFNSLKKVLCEAPVLEFPMSDRMFIIYTDAS 1619
W +F SLK A VL P F I DAS
Sbjct: 787 WPPVALEAFQSLKDAFISASVLRHPEPHLPFFIEVDAS 824 HSP 5 Score: 70.0922 bits (170), Expect = 6.053e-17 Identity = 75/287 (26.13%), Postives = 117/287 (40.77%), Query Frame = -3
Query: 362 QSIKMQ--RRKIKSEVYKQFTRKCKYCGVSKNQNEDIIFKEIIP--LLKGEKETVYDEVK-------QCKYFILIEQLCILSIIEDNKI-LVLVPQNYVTTLINYIHI--ELCHLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTRELL--LPRPGEKFSEQMVIDIAYMETSEWNKKYVIVIIDRFSKLVSLTGTTEQDEITVFNVVLDNWIYRF-GKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAERTIDSKVI*LRHH 1171
QS+K Q R+ S + +F Y +KN+ D + + P L E+E + + Q IL+ Q S D I + VP ++ H + H G KTL ++ +W + + V + C++CA K R LL LP P ++ + I + S N + V+IDRFSK+ + + I+R G P I++DRG F R G+ +F+S Y Q+NG AER + LR+H
Sbjct: 900 QSLKRQNPRQARWSLFFSRFNFVLTYRPGTKNRKADALSRSFSPEDRLPIEQEPIIPPFRIIASVLPQFAEQILLSQSAAPS---DTPIGMAFVPPELRLPILQQTHSSKQAGHPGSEKTLELLRRLVWWPTIRKDVRDFVAACTVCATTKASHSRPCGLLHPLPIPSRPWTHLGMDFIVELPPSCGNT-VIWVVIDRFSKMAHFIPLRKLPSAVELAHLFIQHIFRLHGFPVEIVSDRGSQFVSRFWRSLCKSLGVSLQFSSAYHPQTNGAAERVNQALEQFLRNH 1182 HSP 6 Score: 39.6614 bits (91), Expect = 6.053e-17 Identity = 34/123 (27.64%), Postives = 56/123 (45.53%), Query Frame = -2
Query: 21 ENNWCELLPRIEFCLNAKIQSSTGFSTFDIVYSRK---------INLISNSGNVQELKDEILKSAKVNIENAGNSMKG-SDQDKRGTRILDVGEKVL-----VRLKPHKRKKNGMLYEGPYEI 344
+++W +LLP EF N SSTG S F VY + ++ + + ++ I + K N+E + K +D+ ++ + VGEKV +RLK K G + GP+ I
Sbjct: 1187 QDDWSDLLPWAEFAHNNASHSSTGRSPFLSVYGQHPLAFPQDLLLSEVPAADDLAAHMSVIWAATKSNLEKSSLVHKTFADRRRKPSPPYKVGEKVWLSSRNIRLK-VPSPKLGPKFLGPFSI 1308
BLAST of SMED30024572 vs. Ensembl Xenopus
Match: ENSXETT00000042189.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:KV460667.1:82848:87648:1 gene:ENSXETG00000017765.1 transcript:ENSXETT00000042189.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 65.4698 bits (158), Expect = 5.278e-21 Identity = 42/114 (36.84%), Postives = 63/114 (55.26%), Query Frame = -1
Query: 1777 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*A---LIFKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTI 2109
DL AY + + E + KTAF+TR+ + + +PF L APA FQ + L+ K VVVYLD+ILI +Q E H + E S ++++ L EK F + +F+G+ I
Sbjct: 610 DLRGAYNLIRIREGDEWKTAFNTRDGHYEYLVMPFGLCNAPAVFQEFVNDIFRDLLGKSVVVYLDDILIFSQDLETHRSQVKEALSRLRENFLFAKLEKCTFEVPKISFLGYII 723 HSP 2 Score: 41.2022 bits (95), Expect = 5.278e-21 Identity = 19/51 (37.25%), Postives = 30/51 (58.82%), Query Frame = -3
Query: 2162 SKNYQK--VSKCRSPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMP 2308
S+N Q+ + SP + V +KDG +R C+D+R LN +T K +P+P
Sbjct: 541 SENLQRGFIRPSTSPAGAGFFFVEKKDGGLRPCIDYRGLNKITVKNRYPLP 591 HSP 3 Score: 28.8758 bits (63), Expect = 5.278e-21 Identity = 10/17 (58.82%), Postives = 14/17 (82.35%), Query Frame = -3
Query: 1652 GLTNYYRRFIKDYSPYM 1702
G NYYR+FIKD+S ++
Sbjct: 755 GFANYYRQFIKDFSSHI 771 HSP 4 Score: 26.5646 bits (57), Expect = 5.278e-21 Identity = 14/38 (36.84%), Postives = 15/38 (39.47%), Query Frame = -2
Query: 1506 WNEECKNSFNSLKKVLCEAPVLEFPMSDRMFIIYTDAS 1619
W +F SLK A VL P F I DAS
Sbjct: 787 WPPVALEAFQSLKDAFISASVLRHPEPHLPFFIEVDAS 824 HSP 5 Score: 70.0922 bits (170), Expect = 5.400e-17 Identity = 75/287 (26.13%), Postives = 117/287 (40.77%), Query Frame = -3
Query: 362 QSIKMQ--RRKIKSEVYKQFTRKCKYCGVSKNQNEDIIFKEIIP--LLKGEKETVYDEVK-------QCKYFILIEQLCILSIIEDNKI-LVLVPQNYVTTLINYIHI--ELCHLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTRELL--LPRPGEKFSEQMVIDIAYMETSEWNKKYVIVIIDRFSKLVSLTGTTEQDEITVFNVVLDNWIYRF-GKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAERTIDSKVI*LRHH 1171
QS+K Q R+ S + +F Y +KN+ D + + P L E+E + + Q IL+ Q S D I + VP ++ H + H G KTL ++ +W + + V + C++CA K R LL LP P ++ + I + S N + V+IDRFSK+ + + I+R G P I++DRG F R G+ +F+S Y Q+NG AER + LR+H
Sbjct: 900 QSLKRQNPRQARWSLFFTRFNFVLTYRPGTKNRKADALSRSFSPKDRLPIEQEPIIPPFRIIASVLPQFAEQILLSQSAAPS---DTPIGMAFVPPELRLPILQQTHSSKQAGHPGSEKTLELLRRLVWWPTIRKDVRDFVAACTVCATTKASHSRPCGLLHPLPIPSRPWTHLGMDFIVELPPSCGNT-VIWVVIDRFSKMAHFIPLRKLPSAVELAHLFIQHIFRLHGFPVEIVSDRGSQFVSRFWRSLCKSLGVSLQFSSAYHPQTNGAAERVNQALEQFLRNH 1182 HSP 6 Score: 39.6614 bits (91), Expect = 5.400e-17 Identity = 34/123 (27.64%), Postives = 56/123 (45.53%), Query Frame = -2
Query: 21 ENNWCELLPRIEFCLNAKIQSSTGFSTFDIVYSRK---------INLISNSGNVQELKDEILKSAKVNIENAGNSMKG-SDQDKRGTRILDVGEKVL-----VRLKPHKRKKNGMLYEGPYEI 344
+++W +LLP EF N SSTG S F VY + ++ + + ++ I + K N+E + K +D+ ++ + VGEKV +RLK K G + GP+ I
Sbjct: 1187 QDDWSDLLPWAEFAHNNASHSSTGRSPFLSVYGQHPLAFPQDLLLSEVPAADDLAAHMSVIWAATKSNLEKSSLVHKTFADRRRKPSPPYKVGEKVWLSSRNIRLK-VPSPKLGPKFLGPFSI 1308
BLAST of SMED30024572 vs. UniProt/SwissProt
Match: sp|P04323|POL3_DROME (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1) HSP 1 Score: 85.1149 bits (209), Expect = 2.270e-23 Identity = 51/146 (34.93%), Postives = 83/146 (56.85%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH---*ALIFKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTIK---VYIQR*KIELVKA---PKCVSRLRSFLG 2109
DL + ++Q+E+ ES KTAFST+ + + R+PF L APATFQR + L+ K +VYLD+I++ + + +EH + + VF + K LK+ +K +F K E F+GH + + KIE ++ P +++FLG
Sbjct: 302 DLAKGFHQIEMDPESVSKTAFSTKHGHYEYLRMPFGLKNAPATFQRCMNDILRPLLNKHCLVYLDDIIVFSTSLDEHLQSLGLVFEKLAKANLKLQLDKCEFLKQETTFLGHVLTPDGIKPNPEKIEAIQKYPIPTKPKEIKAFLG 447 HSP 2 Score: 35.8094 bits (81), Expect = 2.270e-23 Identity = 19/50 (38.00%), Postives = 29/50 (58.00%), Query Frame = -3
Query: 2156 VSKCRSPKNSPLVIVGQK-DGS----IRICLDFRRLNAVTDKYSFPMPDM 2290
+ SP NSP+ +V +K D S RI +D+R+LN +T P+P+M
Sbjct: 236 IRTSNSPYNSPIWVVPKKQDASGKQKFRIVIDYRKLNEITVGDRHPIPNM 285 HSP 3 Score: 32.7278 bits (73), Expect = 2.270e-23 Identity = 47/158 (29.75%), Postives = 68/158 (43.04%), Query Frame = -2
Query: 1191 RNNKQIIWNEECKNSFNSLKKVLCEAPVLEFPMSDRMFIIYTDAS---FGAI---DRSCDCIWIKTFDST*NGILFTRKELLLNNEFIVHFRESLWQ*IHCENRS--QSIDFHEHYKKPISPQFQT*LANLSEYDFELQYRK*DDHGNADGFSWIKNE 1640
+N K N E ++F LK ++ E P+L+ P + F + TDAS GA+ D +T + KELL FR L H E S Q + + K P S + LSE+DF+++Y K ++ AD S IK E
Sbjct: 472 KNMKIDTTNPEYDSAFKKLKYLISEDPILKVPDFTKKFTLTTDASDVALGAVLSQDGHPLSYISRTLNEHEINYSTIEKELLAIVWATKTFRHYLLG-RHFEISSDHQPLSWLYRMKDPNS-KLTRWRVKLSEFDFDIKYIKGKENCVADALSRIKLE 627
BLAST of SMED30024572 vs. UniProt/SwissProt
Match: sp|Q99315|YG31B_YEAST (Transposon Ty3-G Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-G PE=1 SV=3) HSP 1 Score: 68.5514 bits (166), Expect = 6.406e-22 Identity = 47/146 (32.19%), Postives = 78/146 (53.42%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*ALIFKG---VVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTI---KVYIQR*KIELVK---APKCVSRLRSFLG 2109
DL Y+Q+ + + + KTAF T ++ + +PF L AP+TF R A F+ V VYLD+ILI +++ EEH + + V +K + L + +K +F E F+G++I K+ + K ++ PK V + + FLG
Sbjct: 686 DLHSGYHQIPMEPKDRYKTAFVTPSGKYEYTVMPFGLVNAPSTFARYM--ADTFRDLRFVNVYLDDILIFSESPEEHWKHLDTVLERLKNENLIVKKKKCKFASEETEFLGYSIGIQKIAPLQHKCAAIRDFPTPKTVKQAQRFLG 829 HSP 2 Score: 48.9062 bits (115), Expect = 6.406e-22 Identity = 19/43 (44.19%), Postives = 30/43 (69.77%), Query Frame = -3
Query: 2162 VSKCRSPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMP 2290
+ +SP +SP+V+V +KDG+ R+C+D+R LN T FP+P
Sbjct: 625 IVPSKSPCSSPVVLVPKKDGTFRLCVDYRTLNKATISDPFPLP 667 HSP 3 Score: 30.8018 bits (68), Expect = 6.406e-22 Identity = 41/152 (26.97%), Postives = 63/152 (41.45%), Query Frame = -2
Query: 1206 WNEECKNSFNSLKKVLCEAPVLEFPMSDRM-FIIYTDAS---FGAIDRSCD---------CIWIKTFDST*NGILFTRKELLLNNEFIVHFRESLWQ*IHCENRSQSIDFHE-HYKKPISPQFQT*LANLSEYDFELQYRK*DDHGNADGFS 1619
W E+ + + LK LC +PVL P +++ + + TDAS GA+ D + K+ +S ELL + + HFR L H R+ I K + + Q L +L+ YDF L+Y + AD S
Sbjct: 858 WTEKQDKAIDKLKDALCNSPVL-VPFNNKANYRLTTDASKDGIGAVLEEVDNKNKLVGVVGYFSKSLESAQKNYPAGELELLGIIKALHHFRYMLHG-KHFTLRTDHISLLSLQNKNEPARRVQRWLDDLATYDFTLEYLAGPKNVVADAIS 1007 HSP 4 Score: 68.5514 bits (166), Expect = 4.299e-10 Identity = 86/382 (22.51%), Postives = 156/382 (40.84%), Query Frame = -3
Query: 386 VIVYGSRHLTAREMGYYSQEKNYYSIMNLLCILE-NLYGNKFIVRTDHKALTFMNTTKNQLAHNFRRSSQTYQNMILSCSIENEMIMEMQMDFHG*KMSFAHNAKQSI----KMQRRKIKSEVYKQFTRKCKYCGVSKNQNEDIIFKEIIP-----LLKGEKETVYDEVKQCKYFILIEQLCILSIIEDNKILVLVPQNYVTTLINYIHIELCHLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTRELLLPRPGEKFSEQMVIDIAY-----METSEWNKKYVIVIIDRFSKLVSLTGTTEQDEITVFNVVLDNWIYRF-GKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAERTIDS 1483
V+ Y S+ L + + Y + E I+ L L+G F +RTDH +L + KN+ A R Q + + + + E + G K A +++ R I +E +K + + C +++ + P +K+ E + Y + E + +D ++ + QN V L + + H G+ TL + +YW L S+I+ I C C K + R LL P P +E +DI+ + + N ++V++DRFSK T + + T +L +I+ + G P +I +DR +E + G++ +S Q++G +ERTI +
Sbjct: 915 VVGYFSKSLESAQKNYPAGELELLGIIKALHHFRYMLHGKHFTLRTDHISLLSLQN-KNEPA----RRVQRWLDDLATYDFTLEYLA-------GPKNVVADAISRAVYTITPETSRPIDTESWKSYYKSDPLCSAVLIHMKELTQHNVTPEDMSAFRSYQKKLELSETFRKNYSLEDEMI----YYQDRLVVPIKQQNAVMRLYHDHTLFGGHFGVTVTLAKISPIYYWPKLQHSIIQYIRTCVQCQLIKSHRPRLHGLLQPLP---IAEGRWLDISMDFVTGLPPTSNNLNMILVVVDRFSKRAHFIATRKTLDATQLIDLLFRYIFSYHGFPRTITSDRDVRMTADKYQELTKRLGIKSTMSSANHPQTDGQSERTIQT 1277
BLAST of SMED30024572 vs. UniProt/SwissProt
Match: sp|Q7LHG5|YI31B_YEAST (Transposon Ty3-I Gag-Pol polyprotein OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TY3B-I PE=1 SV=2) HSP 1 Score: 68.5514 bits (166), Expect = 8.655e-22 Identity = 47/146 (32.19%), Postives = 78/146 (53.42%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*ALIFKG---VVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTI---KVYIQR*KIELVK---APKCVSRLRSFLG 2109
DL Y+Q+ + + + KTAF T ++ + +PF L AP+TF R A F+ V VYLD+ILI +++ EEH + + V +K + L + +K +F E F+G++I K+ + K ++ PK V + + FLG
Sbjct: 712 DLHSGYHQIPMEPKDRYKTAFVTPSGKYEYTVMPFGLVNAPSTFARYM--ADTFRDLRFVNVYLDDILIFSESPEEHWKHLDTVLERLKNENLIVKKKKCKFASEETEFLGYSIGIQKIAPLQHKCAAIRDFPTPKTVKQAQRFLG 855 HSP 2 Score: 48.9062 bits (115), Expect = 8.655e-22 Identity = 19/43 (44.19%), Postives = 30/43 (69.77%), Query Frame = -3
Query: 2162 VSKCRSPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMP 2290
+ +SP +SP+V+V +KDG+ R+C+D+R LN T FP+P
Sbjct: 651 IVPSKSPCSSPVVLVPKKDGTFRLCVDYRTLNKATISDPFPLP 693 HSP 3 Score: 30.4166 bits (67), Expect = 8.655e-22 Identity = 41/152 (26.97%), Postives = 62/152 (40.79%), Query Frame = -2
Query: 1206 WNEECKNSFNSLKKVLCEAPVLEFPMSDRM-FIIYTDAS---FGAIDRSCD---------CIWIKTFDST*NGILFTRKELLLNNEFIVHFRESLWQ*IHCENRSQSIDFHE-HYKKPISPQFQT*LANLSEYDFELQYRK*DDHGNADGFS 1619
W E+ + LK LC +PVL P +++ + + TDAS GA+ D + K+ +S ELL + + HFR L H R+ I K + + Q L +L+ YDF L+Y + AD S
Sbjct: 884 WTEKQDKAIEKLKAALCNSPVL-VPFNNKANYRLTTDASKDGIGAVLEEVDNKNKLVGVVGYFSKSLESAQKNYPAGELELLGIIKALHHFRYMLHG-KHFTLRTDHISLLSLQNKNEPARRVQRWLDDLATYDFTLEYLAGPKNVVADAIS 1033 HSP 4 Score: 68.9366 bits (167), Expect = 3.202e-10 Identity = 87/382 (22.77%), Postives = 156/382 (40.84%), Query Frame = -3
Query: 386 VIVYGSRHLTAREMGYYSQEKNYYSIMNLLCILE-NLYGNKFIVRTDHKALTFMNTTKNQLAHNFRRSSQTYQNMILSCSIENEMIMEMQMDFHG*KMSFAHNAKQSI----KMQRRKIKSEVYKQFTRKCKYCGVSKNQNEDIIFKEIIP-----LLKGEKETVYDEVKQCKYFILIEQLCILSIIEDNKILVLVPQNYVTTLINYIHIELCHLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTRELLLPRPGEKFSEQMVIDIAY-----METSEWNKKYVIVIIDRFSKLVSLTGTTEQDEITVFNVVLDNWIYRF-GKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAERTIDS 1483
V+ Y S+ L + + Y + E I+ L L+G F +RTDH +L + KN+ A R Q + + + + E + G K A ++I R I +E +K + + C +++ + P +K+ E + Y + E + +D ++ + QN V L + + H G+ TL + +YW L S+I+ I C C K + R LL P P +E +DI+ + + N ++V++DRFSK T + + T +L +I+ + G P +I +DR +E + G++ +S Q++G +ERTI +
Sbjct: 941 VVGYFSKSLESAQKNYPAGELELLGIIKALHHFRYMLHGKHFTLRTDHISLLSLQN-KNEPA----RRVQRWLDDLATYDFTLEYLA-------GPKNVVADAISRAIYTITPETSRPIDTESWKSYYKSDPLCSAVLIHMKELTQHNVTPEDMSAFRSYQKKLELSETFRKNYSLEDEMI----YYQDRLVVPIKQQNAVMRLYHDHTLFGGHFGVTVTLAKISPIYYWPKLQHSIIQYIRTCVQCQLIKSHRPRLHGLLQPLP---IAEGRWLDISMDFVTGLPPTSNNLNMILVVVDRFSKRAHFIATRKTLDATQLIDLLFRYIFSYHGFPRTITSDRDVRMTADKYQELTKRLGIKSTMSSANHPQTDGQSERTIQT 1303
BLAST of SMED30024572 vs. UniProt/SwissProt
Match: sp|Q8I7P9|POL5_DROME (Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1) HSP 1 Score: 73.559 bits (179), Expect = 1.584e-21 Identity = 47/146 (32.19%), Postives = 76/146 (52.05%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*AL---IFKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTIKVYIQR*KIELVKA------PKCVSRLRSFLG 2109
DL ++Q+ + E KTAFST ++ F RLPF L APA FQR+ L I K VY+D+I++ ++ + H + + V + + K L++N EK F ++ F+G+ + + + V+A P V L+ FLG
Sbjct: 218 DLTSGFHQIHMKESDIPKTAFSTLNGKYEFLRLPFGLKNAPAIFQRMIDDILREHIGKVCYVYIDDIIVFSEDYDTHWKNLRLVLASLSKANLQVNLEKSHFLDTQVEFLGYIVTADGIKADPKKVRAISEMPPPTSVKELKRFLG 363 HSP 2 Score: 37.3502 bits (85), Expect = 1.584e-21 Identity = 19/46 (41.30%), Postives = 30/46 (65.22%), Query Frame = -3
Query: 2153 SPKNSPLVIVGQK-----DGSIRICLDFRRLNAVTDKYSFPMPDMH 2275
SP NSP+ IV +K + R+ +DF+RLN VT ++P+PD++
Sbjct: 157 SPYNSPIWIVPKKPKPNGEKQYRMVVDFKRLNTVTIPDTYPIPDIN 202 HSP 3 Score: 36.1946 bits (82), Expect = 1.584e-21 Identity = 45/162 (27.78%), Postives = 67/162 (41.36%), Query Frame = -3
Query: 1310 IFGLTNYYRRFIKDYS----PYMLKHRGIISR*FGMKNVKIVLIV*RKFYVKLQYLN----FQCQTECL*F-TLMPVLVLSTEVV------------------IVYGSRHLTAREMGYYSQEKNYYSIM-NLLCILENLYGNKFI-VRTDHKALTFMNTTKN 1708
G+T+YYR+FI+DY+ P RG+ + ++ K+ + + LQ N C +E L F L+T+ I Y SR L E Y + EK +I+ +L + LYG I V TDH+ LTF +N
Sbjct: 361 FLGMTSYYRKFIQDYAKVAKPLTNLTRGLYANIKSSQSSKVPITLDE---TALQSFNDLKSILCSSEILAFPCFTKPFHLTTDASNWAIGAVLSQDDQGRDRPIAYISRSLNKTEENYATIEKEMLAIIWSLDNLRAYLYGAGTIKVYTDHQPLTFALGNRN 519
BLAST of SMED30024572 vs. UniProt/SwissProt
Match: sp|P20825|POL2_DROME (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1) HSP 1 Score: 86.6557 bits (213), Expect = 1.921e-21 Identity = 47/114 (41.23%), Postives = 70/114 (61.40%), Query Frame = -1
Query: 1777 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH---*ALIFKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTI 2109
DL + ++Q+E+ EES KTAFST+ + + R+PF L APATFQR + L+ K +VYLD+I+I + + EH I VF+ + LK+ +K +F K E NF+GH +
Sbjct: 301 DLAKGFHQIEMDEESISKTAFSTKSGHYEYLRMPFGLRNAPATFQRCMNNILRPLLNKHCLVYLDDIIIFSTSLTEHLNSIQLVFTKLADANLKLQLDKCEFLKKEANFLGHIV 414 HSP 2 Score: 36.1946 bits (82), Expect = 1.921e-21 Identity = 20/63 (31.75%), Postives = 35/63 (55.56%), Query Frame = -3
Query: 2156 ISKKLTESKNYQKVSKCRSPKNSPLVIVGQK-DGS----IRICLDFRRLNAVTDKYSFPMPDM 2329
+ ++ E N + + SP NSP +V +K D S R+ +D+R+LN +T +P+P+M
Sbjct: 222 VENQVQEMLNQGLIRESNSPYNSPTWVVPKKPDASGANKYRVVIDYRKLNEITIPDRYPIPNM 284 HSP 3 Score: 23.8682 bits (50), Expect = 1.921e-21 Identity = 14/46 (30.43%), Postives = 24/46 (52.17%), Query Frame = -2
Query: 2376 LNFEKKSFFGQIISEFRNIFMASRFDLGYTKIIQHEIKTV-GSPIF 2510
LN E+ ++++FRN+ L +T I+H + T SPI+
Sbjct: 163 LNQEETFKLKGLLNKFRNLEYKEGEKLTFTNTIKHVLNTTHNSPIY 208
BLAST of SMED30024572 vs. TrEMBL
Match: A0A087T3I4 (Retrovirus-related Pol polyprotein from transposon 412 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_13332 PE=4 SV=1) HSP 1 Score: 112.464 bits (280), Expect = 4.143e-56 Identity = 123/485 (25.36%), Postives = 216/485 (44.54%), Query Frame = -3
Query: 398 FGLTNYYRRFIKDYSPYMLK-HRGIISR*---------FGMKNVKIVLIV*RKF-YVKLQYLNFQCQTECL*FTLMPVL---VLSTEVVIVYGSRHLTAREMGYYSQEKNYYSIMNLLCILEN-LYGNKFIVRTDHKALTFMNTTKNQLAHNFRRSSQTYQNMILSCSIENEMIMEMQMDFHG*KMSFAH-----NAKQSIKMQRR-KIKSEVYKQFTRKCKYCG-------VSKNQNEDIIFKEIIPLLK-GEKETVYDEVKQC-----KYFILIEQLCILSII-------EDNKIL---VLVPQNYVTTLINYIHIELC--HLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTR-ELLLPRPGEKFSEQMVIDI--AYMETSEWNKKYVIVIIDRFSKLVSLTGTTEQDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAER 1705
GL+ YYRRF+KD+S H+ S+ KN+K L Y KL F T+ ++ VL + E V+ Y S+ L+ E Y K +I+ + + LYG KF++RTDH +L ++ NF+ I + +I + HG + + N + +++ + ++ + V +Q T + K+Q ED K II L++ ++ + ++ +Y+ L + L I + + +D K +++P++ + ++ +H H GI+KTL +++ FYWNN V K C CA RK + R+R +L G F E++ DI T++ NK Y++V ID F+K +Q+ +TV +++ +WI RFG P + +D+G+ F + +E G+ + +P QS+G+ ER
Sbjct: 918 LGLSTYYRRFVKDFSSIARPLHKLTESKQKFVWTKECENAFKNLKEALTSAPILTYPKLDR-PFILDTDASNESVGAVLSQEIEGQERVVAYWSKCLSKPERNYCVTRKELLAIVKAVEHFHHYLYGRKFLLRTDHASLAWL--------LNFKNPEGQIARWIQRLQEYDIIIRHRKGQSHGNADALSRRPCPENCRYCSRVEAKYQLVNPVARQITASTSADPDPWSDKEIRKDQMEDRDIKPIIELMETSNRKPTWQDISSYSPTTKQYWALWDSLHIRNGVLYRKFESDDGKTFRWQLVLPRSRIPDVLKELHSSPAGGHFGIMKTLQRVRERFYWNNAKDDVQKWCRTCDACASRKGPKKRSRGKLQRYNVGAPF-ERIAFDILGPLPRTADGNK-YILVAIDYFTKWPEAYPIPDQEAVTVAEMMIQHWISRFGVPLQLHSDQGRNFTSAVVKELCKLLGIDKTQTTPLHPQSDGMVER 1391 HSP 2 Score: 88.5817 bits (218), Expect = 4.143e-56 Identity = 53/146 (36.30%), Postives = 83/146 (56.85%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*---ALIFKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTIKVYIQR*KIELVKA------PKCVSRLRSFLG 2109
DL Y+QVE+ + + KTAF+T + + F +PF L APATF+RL L ++ ++YLD+I+I ++ EEH + +V +K+ LK++T K + ++ E ++GH I R E V A P+ V +LRSFLG
Sbjct: 774 DLKSGYWQVEIHPDDREKTAFTTGQGLWQFKVMPFGLCNAPATFERLMETVLKGLSYEACLIYLDDIIIVGKSFEEHLENLRKVLQKLKEANLKLSTAKCKLFRQEVTYLGHVISAEGVRTDPEKVSAVKHWRRPENVHQLRSFLG 919 HSP 3 Score: 54.6842 bits (130), Expect = 4.143e-56 Identity = 23/38 (60.53%), Postives = 30/38 (78.95%), Query Frame = -3
Query: 2162 SPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMP 2275
SP SP+V+V +KDGS R C+D+RRLN VT K S+P+P
Sbjct: 718 SPWASPIVLVRKKDGSTRFCVDYRRLNEVTKKDSYPLP 755 HSP 4 Score: 33.113 bits (74), Expect = 4.143e-56 Identity = 15/46 (32.61%), Postives = 26/46 (56.52%), Query Frame = -2
Query: 2373 LNFEKKSFFGQIISEFRNIFMASRFDLGYTKIIQHEIKTVGSPIFR 2510
LN E+K+ ++I + +++F S D+G T + QH I T P +
Sbjct: 641 LNDEEKNAAERVIKDLQDVFSRSSSDVGRTSLTQHRIDTGDHPPIK 686 HSP 5 Score: 23.483 bits (49), Expect = 4.143e-56 Identity = 8/38 (21.05%), Postives = 19/38 (50.00%), Query Frame = -2
Query: 231 ENNWCELLPRIEFCLNAKIQSSTGFSTFDIVYSRKINL 344
+ +W + LP + + +TG+S +++ R + L
Sbjct: 1408 QQDWDKKLPLFLLAYRSAVHETTGYSPSQMLFGRDLRL 1445 HSP 6 Score: 73.559 bits (179), Expect = 4.750e-9 Identity = 55/179 (30.73%), Postives = 83/179 (46.37%), Query Frame = -2
Query: 1206 LSTFY*RLFTLYAET*R-------NNKQIIWNEECKNSFNSLKKVLCEAPVLEFPMSDRMFIIYTDA---SFGAI-------DRSCDCIWIKTFDST*NGILFTRKELLLNNEFIVHFRESLW-Q*IHCENRSQSIDFHEHYKKPISPQFQT*LANLSEYDFELQYRK*DDHGNADGFS 1688
LST+Y R ++ R + ++ +W +EC+N+F +LK+ L AP+L +P DR FI+ TDA S GA+ W K TRKELL + + HF L+ + S+ + ++K P Q + L EYD +++RK HGNAD S
Sbjct: 920 LSTYYRRFVKDFSSIARPLHKLTESKQKFVWTKECENAFKNLKEALTSAPILTYPKLDRPFILDTDASNESVGAVLSQEIEGQERVVAYWSKCLSKPERNYCVTRKELLAIVKAVEHFHHYLYGRKFLLRTDHASLAWLLNFKNP-EGQIARWIQRLQEYDIIIRHRKGQSHGNADALS 1097
BLAST of SMED30024572 vs. TrEMBL
Match: A0A5S6QHB1 (Uncharacterized protein OS=Trichuris muris OX=70415 PE=4 SV=1) HSP 1 Score: 101.679 bits (252), Expect = 4.487e-55 Identity = 117/502 (23.31%), Postives = 194/502 (38.65%), Query Frame = -3
Query: 377 FGLTNYYRRFIKDYSPYMLKHRGIISR*----------FGMKNVKIVLIV*RKFYVKLQYLNFQCQTECL*FTLMPVLVL----STEVVIVYGSRHLTAREMGYYSQEKNYYSIMNLLCILENLYGNKFIVRTDHKALTFMNTTKNQLAHNFRRSSQTYQNMILSCSIENEMIMEMQMDFHG*KMSFAHNAKQSIKMQRRKIKSEVYKQFTRKCKYCGVSKNQ------------------NEDIIFKEIIPLLKGEKETVYDEVKQC------KYFILI--EQLCILSIIEDNKIL----------VLVPQNYVTTLINYIHIELC--HLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTRELLLPRPGEKFSEQMVIDI-AYMETSEWNKKYVIVIIDRFSKLVSLTGTTEQDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAER------TIDSKVI 1705
GL +YYR+FIKD++ + + + ++ L+ + +F T+ + VL E + Y SR LT E Y + +++ KF +RTDH +L ++ KN + R Q Q Q DF H A R ++ + CK CG S + + ++I P+LK + V + +C K +++ +L I I K V+VP+ + +++ H +L HLGI KT+ +++ FYW K + C C R G+ R L P + E++ IDI + SE +Y++V ID FSK +Q+ TV V+++ +I R+G P++I TD+G FE + + + G+R+ +PY NG ER T+ SKV+
Sbjct: 785 LGLASYYRKFIKDFAAIAKPLHQLTEKRKPFQWTPECTSAFQKLRTALLSEPILRLPDFDASFILDTDASDTAIGAVLSQRDEHGREHPVAYASRTLTRAEQRYCVTRREMLAVITFTDQFRPYLQQKFTLRTDHGSLQWLRDFKNPDGQ-WARWQQKLQ----------------QYDF-----DIEHRAGS------RHANADTLSRIP--CKQCGRSGTEVMGVPVNVVALENLEEMRTSQLDDEDIAPILKAKAAGVVGQEIRCGKRSNSKNLLMLNWHRLAIQKGILVRKWFCDDQSGYRWQVVVPKRMIKPVLDQAHQQLTAGHLGIEKTIERIRERFYWPGYRSDTKKYVASCYECNTRNEPVGKGRAPLQPVVTTRRWEKLAIDILGPLVVSETGNRYILVAIDCFSKFAEAFPIPDQEAKTVTRVLVNEFICRYGVPEAIHTDQGSQFEAAIFQSMCTELGIRKTRTTPYHPSGNGQVERMNRTLGTMLSKVV 1256 HSP 2 Score: 90.8929 bits (224), Expect = 4.487e-55 Identity = 54/146 (36.99%), Postives = 85/146 (58.22%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*ALI---FKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTI-KVYIQ-----R*KIELVKAPKCVSRLRSFLG 2109
DL Y+QVEL+E ++ KTAF T + F FN +PF L+ APATFQRL L + +VYLD+I++ ++T EEH + +V + ++K GLK N K + + E ++GH + + I+ K+ P C++ ++ FLG
Sbjct: 641 DLLSGYWQVELTEAAKEKTAFITHDGLFQFNVMPFGLTGAPATFQRLMEHVLAGLKWNTCLVYLDDIIVFSRTAEEHVEHLSQVLNRLQKAGLKANASKCKLFCKEVRYLGHIVSEKGIEPDPSLTEKMRTYPVPTCLAEVKRFLG 786 HSP 3 Score: 45.8246 bits (107), Expect = 4.487e-55 Identity = 19/41 (46.34%), Postives = 31/41 (75.61%), Query Frame = -3
Query: 2162 PKNSP----LVIVGQKDGSIRICLDFRRLNAVTDKYSFPMP 2272
P NSP +V V +KDGS+R+C+D+R+LN V+ + ++P+P
Sbjct: 582 PSNSPWAAGIVPVRKKDGSLRLCVDYRKLNEVSRRDAYPIP 622 HSP 4 Score: 39.2762 bits (90), Expect = 4.487e-55 Identity = 17/46 (36.96%), Postives = 29/46 (63.04%), Query Frame = -2
Query: 2394 EKVALRILNFEKKSFFGQIISEFRNIFMASRFDLGYTKIIQHEIKT 2531
E + + ++K G ++S+FRN F AS FD+G T +++H+I T
Sbjct: 502 ETTGVPLTASQRKKLHG-MLSKFRNAFAASEFDIGRTSVLKHDIVT 546 HSP 5 Score: 31.187 bits (69), Expect = 4.487e-55 Identity = 17/62 (27.42%), Postives = 29/62 (46.77%), Query Frame = -2
Query: 171 NNWCELLPRIEFCLNAKIQSSTGFSTFDIVYSRKINLISN-----SGNVQELKDEILKSAKV 341
W E+LP++ A IQSST + + +V+ + L + G VQ ++ + KV
Sbjct: 1261 RKWDEVLPKVMMAYRASIQSSTRMAPYTMVFGEQCRLPEDIYRPAEGKVQSPEEHATQLKKV 1322
BLAST of SMED30024572 vs. TrEMBL
Match: A0A5S6Q2I3 (Uncharacterized protein OS=Trichuris muris OX=70415 PE=4 SV=1) HSP 1 Score: 98.9821 bits (245), Expect = 3.034e-54 Identity = 116/502 (23.11%), Postives = 193/502 (38.45%), Query Frame = -3
Query: 377 FGLTNYYRRFIKDYSPYMLKHRGIISR*----------FGMKNVKIVLIV*RKFYVKLQYLNFQCQTECL*FTLMPVLVL----STEVVIVYGSRHLTAREMGYYSQEKNYYSIMNLLCILENLYGNKFIVRTDHKALTFMNTTKNQLAHNFRRSSQTYQNMILSCSIENEMIMEMQMDFHG*KMSFAHNAKQSIKMQRRKIKSEVYKQFTRKCKYCGVSKNQ------------------NEDIIFKEIIPLLKGEKETVYDEVKQC------KYFILI--EQLCILSIIEDNKIL----------VLVPQNYVTTLINYIHIELC--HLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTRELLLPRPGEKFSEQMVIDI-AYMETSEWNKKYVIVIIDRFSKLVSLTGTTEQDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAER------TIDSKVI 1705
GL +YYR+FIKD++ + + + ++ L+ + +F T+ + VL E + Y SR LT E Y + +++ KF +RTDH +L ++ KN + R Q Q Q DF H A R ++ + CK CG S + + ++I P+LK + V + +C K +++ +L I I K V+VP+ + +++ H +L HLGI KT+ +++ FYW K + C C R + R L P + E++ IDI + SE +Y++V ID FSK +Q+ TV V+++ +I R+G P++I TD+G FE + + + G+R+ +PY NG ER T+ SKV+
Sbjct: 785 LGLASYYRKFIKDFAAIAKPLHQLTEKRKPFQWTPECTSAFQKLRTALLSEPILRLPDFDASFILDTDASDTAIGAVLSQRDEHGREHPVAYASRTLTRAEQRYCVTRREMLAVITFTDQFRPYLQQKFTLRTDHGSLQWLRDFKNPDGQ-WARWQQKLQ----------------QYDF-----DIEHRAGS------RHANADTLSRIP--CKQCGRSGTEVMGVPVNVVALENLEEMRTSQLDDEDIAPILKAKAAGVVGQEIRCGKRSNSKNLLMLNWHRLAIQKGILVRKWFCDDQSGYRWQVVVPKRMIKPVLDQAHQQLTAGHLGIEKTIERIRERFYWPGYRSDTKKYVASCYECNTRNEPVEKGRAPLQPVVATRRWEKLAIDILGPLVVSETGNRYILVAIDCFSKFAEAFPIPDQEAKTVTRVLVNEFICRYGVPEAIHTDQGSQFEAAIFQSMCTELGIRKTRTTPYHPSGNGQVERMNRTLGTMLSKVV 1256 HSP 2 Score: 90.5077 bits (223), Expect = 3.034e-54 Identity = 54/146 (36.99%), Postives = 85/146 (58.22%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*ALI---FKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTI-KVYIQ-----R*KIELVKAPKCVSRLRSFLG 2109
DL Y+QVEL+E ++ KTAF T + F FN +PF L+ APATFQRL L + +VYLD+I++ ++T EEH + +V + ++K GLK N K + + E ++GH + + I+ K+ P C++ ++ FLG
Sbjct: 641 DLLSGYWQVELTEAAKEKTAFITHDGLFQFNVMPFGLTGAPATFQRLMEHVLAGLKWNTCLVYLDDIIVFSRTAEEHVEHLSQVLNRLQKAGLKPNASKCKLFCKEVRYLGHIVSEKGIEPDPSLTEKMRTYPVPTCLAEVKRFLG 786 HSP 3 Score: 45.8246 bits (107), Expect = 3.034e-54 Identity = 19/41 (46.34%), Postives = 31/41 (75.61%), Query Frame = -3
Query: 2162 PKNSP----LVIVGQKDGSIRICLDFRRLNAVTDKYSFPMP 2272
P NSP +V V +KDGS+R+C+D+R+LN V+ + ++P+P
Sbjct: 582 PSNSPWAAGIVPVRKKDGSLRLCVDYRKLNEVSRRDAYPIP 622 HSP 4 Score: 39.2762 bits (90), Expect = 3.034e-54 Identity = 17/46 (36.96%), Postives = 29/46 (63.04%), Query Frame = -2
Query: 2394 EKVALRILNFEKKSFFGQIISEFRNIFMASRFDLGYTKIIQHEIKT 2531
E + + ++K G ++S+FRN F AS FD+G T +++H+I T
Sbjct: 502 ETTGVPLTASQRKKLHG-MLSKFRNAFAASEFDIGRTSVLKHDIVT 546 HSP 5 Score: 31.5722 bits (70), Expect = 3.034e-54 Identity = 17/62 (27.42%), Postives = 29/62 (46.77%), Query Frame = -2
Query: 171 NNWCELLPRIEFCLNAKIQSSTGFSTFDIVYSRKINLISN-----SGNVQELKDEILKSAKV 341
W E+LP++ A IQSST + + +V+ + L + G VQ ++ + KV
Sbjct: 1261 RKWDEVLPKVMMAYRASIQSSTRMAPYTMVFGEQCRLPEDIYRPAEGKVQSPEEHATQLKKV 1322
BLAST of SMED30024572 vs. TrEMBL
Match: A0A5G3IEJ4 (Uncharacterized protein OS=Xenopus tropicalis OX=8364 PE=4 SV=1) HSP 1 Score: 98.2117 bits (243), Expect = 1.093e-53 Identity = 58/146 (39.73%), Postives = 87/146 (59.59%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRL---TH*ALIFKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTIK---VYIQR*KIELVKA---PKCVSRLRSFLG 2109
DL YYQ+ + E + KTAF F FNR+P L APATFQRL T + V+VYLD++++ +T EEH++ + +VF ++K+GLK++ EK QF + N++GH + + KIE V + P+ ++ LRSFLG
Sbjct: 416 DLRSGYYQLPMHPEDKEKTAFICPLGFFEFNRMPQGLCGAPATFQRLMERTVGDMHLLEVLVYLDDLIVFGRTLEEHEQRLLKVFDRLEKEGLKLSPEKCQFCQPSVNYVGHVVSAEGIATDPSKIEAVSSWPKPRTITELRSFLG 561 HSP 2 Score: 84.3445 bits (207), Expect = 1.093e-53 Identity = 87/412 (21.12%), Postives = 169/412 (41.02%), Query Frame = -3
Query: 398 IVYGSRHLTAREMGYYSQEKNYYSIMNLLC--ILENLYGNKFIVRTDHKALTFMNTTK--NQLAHNFRRSSQTYQ-NMILSCSIENEMIMEMQMDFHG*KMSFAHNAKQSIKMQRRKIKSEVYKQFTRKCK------------------YCGVS----------------KNQNEDIIFKEIIPLLKGEKETVYDEVKQCKYFILIEQLCIL------------SIIEDNKILVLVPQNYVTTLINYIHIELCHLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTRELLLPRPGEKFSEQMVIDIAYMETSEWNKKYVIVIIDRFSKLVSLTGTTEQDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAER 1480
+ + SR L+ E Y + + + ++ + + E LYG +F V+TD+ LT++ TT + H + + Y+ ++ +N + HG H + I++ +++ + + T +C+ YC V+ ++Q+ED + + + L+ ++ + +L+++ L S + K +++PQ + +++ +H E HLG KTL ++D FYW + Q V C C +RK R L + + ID +E E V+V+ D +++ T +Q ITV V+++ + +G P I +D+G+ FE E M G+ + +PY Q + ER
Sbjct: 672 VAFISRSLSPSERNYPAHKLEFLALKWAVVDKLHEYLYGAEFEVQTDNNPLTYILTTAKLDATGHRWLAALAHYKFSLRYRPGRDNRDADGLSRRPHG----DLHPDDEWIEIPAPGVRT-MCQGITGRCQSGNFAEKIGMTVRGIPKLYCNVTSVGTSTMPALNKGDIKRDQSEDPLCQMAMEALRKKQVQILKTDSHPLASLLVKEWDRLRLRDGLVYRRAPSATDSEKWQLMLPQKHRDSVLMALHDEHGHLGYDKTLGLVRDRFYWPCMKQDVEDYCRSCLRCIQRKTLPSRAAPLSHMESHSPL-DLVCIDFLSIEPDEGGTSNVLVVTDHYTRYAQAFPTKDQRAITVAKVLVERFFIHYGLPKRIHSDQGRDFESKLVHELMSMLGVLKSRTTPYHPQGDPQPER 1077 HSP 3 Score: 49.2914 bits (116), Expect = 1.093e-53 Identity = 24/56 (42.86%), Postives = 37/56 (66.07%), Query Frame = -3
Query: 2168 ISKKLTESKNYQKVSKCRSPKNSPLVIVGQKDGSIRICLDFRRLN--AVTDKYSFP 2329
+ K L E K + + RSP SP+V+V +K+GSIR+C+D+R LN + D+Y+ P
Sbjct: 342 LRKHLEELKAAGIIKESRSPYASPIVVVRKKNGSIRMCVDYRTLNQRTIPDQYTTP 397 HSP 4 Score: 38.891 bits (89), Expect = 1.093e-53 Identity = 16/45 (35.56%), Postives = 26/45 (57.78%), Query Frame = -2
Query: 1506 RNNKQIIWNEECKNSFNSLKKVLCEAPVLEFPMSDRMFIIYTDAS 1640
R + + W EEC +F+ LK L APVL + + + + ++ DAS
Sbjct: 609 RESIEDFWTEECDKAFDQLKYCLTHAPVLAYADATKPYTLHIDAS 653 HSP 5 Score: 27.7202 bits (60), Expect = 1.093e-53 Identity = 23/108 (21.30%), Postives = 48/108 (44.44%), Query Frame = -2
Query: 78 KENNWCELLPRIEFCLNAKIQSSTGFSTFDIVYSRKINLI---------------SNSGNVQELKDEILKSAKVNIENAG-NSMKGSDQDKRGTRILDV--GEKVLVR 347
K+ +W + + N+ +TG+S + +++ R+ L S+S V LK ++ ++ K E G +M+ + +I D+ G++VL+R
Sbjct: 1093 KKTHWSRHIATVVHAYNSTKNGATGYSPYFLMFGREARLPIDTVFGVTADDTPVKSHSSYVDRLKRDLQRAYKRAQEAVGKKNMQNKTLYDKKVKIHDLQPGDRVLLR 1200 HSP 6 Score: 24.2534 bits (51), Expect = 1.093e-53 Identity = 8/15 (53.33%), Postives = 10/15 (66.67%), Query Frame = -3
Query: 1661 FGLTNYYRRFIKDYS 1705
G YYRRF+K +S
Sbjct: 560 LGFCGYYRRFVKGFS 574 HSP 7 Score: 21.1718 bits (43), Expect = 1.093e-53 Identity = 12/49 (24.49%), Postives = 24/49 (48.98%), Query Frame = -2
Query: 2367 ILNFEKKSFFGQIISEFRNIFMASRFDLGYTKIIQHEIKTVGSPIFRTR 2513
++ E KS + ++E +F + D+G + +H+I+ FR R
Sbjct: 282 VIPPEWKSRLRKHLNEHSALFSRNDLDMGCSTSTKHKIRLREDKPFRER 330
BLAST of SMED30024572 vs. TrEMBL
Match: A0A087SUZ9 (Retrovirus-related Pol polyprotein from transposon 412 (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_16630 PE=4 SV=1) HSP 1 Score: 105.531 bits (262), Expect = 2.326e-53 Identity = 120/484 (24.79%), Postives = 212/484 (43.80%), Query Frame = -3
Query: 398 FGLTNYYRRFIKDYSPYMLK-HRGIISR*---------FGMKNVKIVLIV*RKFYVKLQYLNFQCQTECL*FTLMPVL---VLSTEVVIVYGSRHLTAREMGYYSQEKNYYSIMNLLCILEN-LYGNKFIVRTDHKALTFMNTTKNQLAHNFRRSSQTYQNMILSCSIENEMIMEMQMDFHG*KMSFAH-----NAKQSIKMQRR-KIKSEVYKQFTRKCKYCG-------VSKNQNEDIIFKEIIPLLK-GEKETVYDEVKQC-----KYFILIEQLCILSII-------EDNKIL---VLVPQNYVTTLINYIHIELC--HLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTR-ELLLPRPGEKFSEQMVIDI--AYMETSEWNKKYVIVIIDRFSKLVSLTGTTEQDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAER 1705
GL YYRRF+KD+S H+ S+ KN+K L F T+ ++ VL + E V+ Y S+ L+ E Y K +I+ + + LYG KF++RTDH +L ++ KN R Q Q ++ I + HG + + N + +++ + ++ + V +Q T + K+Q +D K II L++ ++ + ++ +Y+ L + L I + + +D K +++P++ + ++ +H H GI+KTL +++ FYWNN V K C C RK + R+R +L G F E++ DI T++ NK Y++V ID F+K +Q+ +TV +++ +WI RFG P + +D+G+ F + +E G+ + +P QS+G+ ER
Sbjct: 904 LGLCTYYRRFVKDFSSIARPLHKLTESKQKFVWTKECENAFKNLKEALTSAPILTYPQLDRPFILDTDASNESVGAVLSQEIEGQERVVAYWSKCLSKPERNYCVTRKELLAIVKAVEHFHHYLYGRKFLLRTDHASLAWLLNFKNPEGQ-IARWIQRLQEYDIT-------IRHRKGQSHGNADALSRRPCPENCRYCSRVEAKYQLVNPVARQITASTLADPDPWTDKEIRKDQLQDRDIKPIIELMETSNRKPTWQDISSYSPTTKQYWALWDSLHIRNGVLYRKFESDDGKTFRWQLVLPRSRIPDVLKELHSSPAGGHFGIMKTLQRVRERFYWNNAKDDVQKWCRTCDACVSRKGPKKRSRGKLQRYNVGAPF-ERIAFDILGPLPRTADGNK-YILVAIDYFTKWPEAYPIPDQEAVTVAEMMIQHWISRFGVPLQLHSDQGRNFTSAVVKELCKLLGIDKTQTTPLHPQSDGMVER 1377 HSP 2 Score: 86.2705 bits (212), Expect = 2.326e-53 Identity = 52/146 (35.62%), Postives = 82/146 (56.16%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH---*ALIFKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTIKVYIQR*KIELVKA------PKCVSRLRSFLG 2109
DL Y+QVE+ + + KTAF+T + + F +PF L APATF+RL L ++ ++YLD+I+I ++ EEH + +V +K+ LK++ K + ++ E ++GH I R E V A P+ V +LRSFLG
Sbjct: 760 DLKSGYWQVEIHPDDREKTAFTTGQGLWQFKVMPFGLCNAPATFERLMETVLKGLSYEACLIYLDDIIIVGKSFEEHLENLRKVLQKLKEANLKLSPAKCKLFRQEVTYLGHVISAEGVRTDPEKVSAVKDWRRPENVHQLRSFLG 905 HSP 3 Score: 55.0694 bits (131), Expect = 2.326e-53 Identity = 23/38 (60.53%), Postives = 30/38 (78.95%), Query Frame = -3
Query: 2162 SPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMP 2275
SP SP+V+V +KDGS R C+D+RRLN VT K S+P+P
Sbjct: 704 SPWASPIVLVRKKDGSTRFCVDYRRLNEVTKKDSYPLP 741 HSP 4 Score: 32.7278 bits (73), Expect = 2.326e-53 Identity = 15/46 (32.61%), Postives = 26/46 (56.52%), Query Frame = -2
Query: 2373 LNFEKKSFFGQIISEFRNIFMASRFDLGYTKIIQHEIKTVGSPIFR 2510
LN E+K+ ++I + +++F S D+G T + QH I T P +
Sbjct: 627 LNDEEKNAAERVIKDLQDVFSRSSSDVGRTSLTQHRIDTGDHPPIK 672 HSP 5 Score: 23.8682 bits (50), Expect = 2.326e-53 Identity = 8/38 (21.05%), Postives = 19/38 (50.00%), Query Frame = -2
Query: 231 ENNWCELLPRIEFCLNAKIQSSTGFSTFDIVYSRKINL 344
+ +W + LP + + +TG+S +++ R + L
Sbjct: 1394 QQDWDKKLPLFLLAYRSAVHETTGYSPSQMLFGRDLRL 1431 HSP 6 Score: 73.559 bits (179), Expect = 4.256e-9 Identity = 49/155 (31.61%), Postives = 74/155 (47.74%), Query Frame = -2
Query: 1206 NNKQIIWNEECKNSFNSLKKVLCEAPVLEFPMSDRMFIIYTDA---SFGAI-------DRSCDCIWIKTFDST*NGILFTRKELLLNNEFIVHFRESLW-Q*IHCENRSQSIDFHEHYKKPISPQFQT*LANLSEYDFELQYRK*DDHGNADGFS 1637
+ ++ +W +EC+N+F +LK+ L AP+L +P DR FI+ TDA S GA+ W K TRKELL + + HF L+ + S+ + ++K P Q + L EYD +++RK HGNAD S
Sbjct: 930 SKQKFVWTKECENAFKNLKEALTSAPILTYPQLDRPFILDTDASNESVGAVLSQEIEGQERVVAYWSKCLSKPERNYCVTRKELLAIVKAVEHFHHYLYGRKFLLRTDHASLAWLLNFKNP-EGQIARWIQRLQEYDITIRHRKGQSHGNADALS 1083
BLAST of SMED30024572 vs. Ensembl Cavefish
Match: ENSAMXT00000035136.1 (pep primary_assembly:Astyanax_mexicanus-2.0:19:22120237:22124307:1 gene:ENSAMXG00000032783.1 transcript:ENSAMXT00000035136.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 91.6633 bits (226), Expect = 2.536e-49 Identity = 54/146 (36.99%), Postives = 89/146 (60.96%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*A---LIFKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTIK---VYIQR*KIELVK---APKCVSRLRSFLG 2109
DL YYQVE+ E+ + KTAF + +NRLP ++ AP+TFQRL + K V+V+LD+++I +++ EEH+ + +V + +K+ GLK++ EK +F+++ ++GH + V KI +K +PK + LRSFLG
Sbjct: 204 DLKSGYYQVEMEEDDKPKTAFVCPLGFWEWNRLPQGITNAPSTFQRLMERCVGEMNLKEVLVFLDDLIIFSKSLEEHEERLLKVLNCLKEYGLKLSPEKCKFFQTSVRYLGHIVSENGVKTDPDKIAALKNWPSPKNLKELRSFLG 349 HSP 2 Score: 89.7373 bits (221), Expect = 2.536e-49 Identity = 131/547 (23.95%), Postives = 204/547 (37.29%), Query Frame = -3
Query: 413 FGLTNYYRRFIKDYS----PYMLKHRGIISR*FGMKNVKIVLIV*RKFYVKLQYLN----FQ------CQ----TECL*FTLMPVL---------VLSTEV------------------VIVYGSRHLTAREMGYYSQEKNYYSIMNLLC--ILENLYGNKFIVRTDHKALTFMNTTKNQLAHNFR---------------RSSQTYQNMILSCSIENEMI-----------MEMQMDFHG*KMSFA-------------HNAKQ--------SIKMQRRKIKSEVYKQ-------FTRKCKYCGVSKNQNEDIIFKEIIPLLK-GEKETVYDEVKQCKYFILIEQLCILSII---------EDNKIL--VLVPQNYVTTLINYIHIELCHLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTREL---LLPRPGEKFSEQMVIDIAYMETSEWNKKYVIVIIDRFSKLVSLTGTTEQDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSN 1705
G + YYRRF+KDYS P G + G K K YLN FQ CQ T T PVL +L T+ VI + SR LT E Y + + + ++ + + LYGN F V TD LT++ T+ A ++R R Q LS E+I + M +H ++ H A Q S+ + R I E+ ++ K +++ Q D +I L+ GEK + F+L + L +I E+N+ L +++P ++ +H E+ H+G+ +TL ++ FYW ++ V K I+ C C +RK + L L RP E + ID +E N K V+VI D F+K T Q TV + D ++ +G P+ + +D+G FE + +E G+R+ SPY + N
Sbjct: 348 LGFSGYYRRFVKDYSKIAKPLNALTSGYPPQRKGAK----------KPETNKNYLNPKEPFQGRWTASCQQAFETIIEKLTTAPVLGFANPKLPYILHTDASTTGLGAALYQDQGGEKRVIAFASRGLTCSEARYPAHKLEFLALKWAVVDKFSDYLYGNTFTVVTDSNPLTYILTSAKLDATSYRWLSALSTFSFDLQYRRGKQNLDADGLSRRPHGELINDSISQKEHERIRQFMQYHQEELVDQKSDHEVVKAICERHLATQQSSIALVESLALHARAIPEELMEEPFHLGHGVPFKFSNAEIAEKQRADQTLAPVINQLETGEKPPPTLRQELPDIFLLFREWNKLEMIDGVLYRRRQEENRNLYQLVLPVELRPIVLQSLHDEMGHMGVERTLDLVRSRFYWPKMAIDVEKKIHTCGRCVRRKALPDKAAPLVNILTTRP----LELVCIDFLSIEPDSKNTKDVLVITDHFTKYAIAVPTPNQKARTVAKCLWDTFVVHYGLPERLHSDQGPDFESTLIKELCALVGVRKCRTSPYHPRGN 880 HSP 3 Score: 47.3654 bits (111), Expect = 2.536e-49 Identity = 20/58 (34.48%), Postives = 39/58 (67.24%), Query Frame = -3
Query: 2156 ISKKLTESKNYQKVSKCRSPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMPDM 2329
+ K L E + + + SP +SP+V+V +K+G +R+C+D+R+LN T K ++ +P++
Sbjct: 130 VRKHLKELSDAGIIRESESPFSSPIVVVRKKNGDVRLCVDYRKLNLQTIKDAYALPNL 187 HSP 4 Score: 27.335 bits (59), Expect = 2.536e-49 Identity = 24/108 (22.22%), Postives = 49/108 (45.37%), Query Frame = -2
Query: 78 KENNWCELLPRIEFCLNAKIQSSTGFSTFDIVYSRKINL---------------ISNSGNVQELKDEILKSAKVNIENAGNSMKGSDQ--DKRGTR-ILDVGEKVLVR 347
++++W + + + N TGF+ +++++ R+ L S+S + LK + +S ++ + A + DKR T L+VG++VLVR
Sbjct: 900 EKSHWSDFVKSLVHAYNCTKNEVTGFTPYELMFGRRPRLPVDIAFGLPLEKNQNQSHSQYMTVLKAHLEESYRLATQAAAKIAHKNKVKFDKRVTESTLNVGDRVLVR 1007 HSP 5 Score: 23.0978 bits (48), Expect = 2.536e-49 Identity = 9/31 (29.03%), Postives = 15/31 (48.39%), Query Frame = -2
Query: 2364 IFMASRFDLGYTKIIQHEIKTVGSPIFRTRV 2456
+F D G+T+ ++H IK F+ R
Sbjct: 89 VFSLHDLDFGHTQAVKHYIKLHDERPFKHRA 119
BLAST of SMED30024572 vs. Ensembl Cavefish
Match: ENSAMXT00000041345.1 (pep primary_assembly:Astyanax_mexicanus-2.0:25:32334536:32339002:1 gene:ENSAMXG00000029230.1 transcript:ENSAMXT00000041345.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 73.559 bits (179), Expect = 2.057e-44 Identity = 50/146 (34.25%), Postives = 77/146 (52.74%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*ALI---FKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTIK---VYIQR*KIELVKA---PKCVSRLRSFLG 2109
DL Y+QV L +S KTAF T + + F LPF + A ATFQRL L+ K VY+++I++ + T E+H + EVF + + GL +N K + F+GH I + + K+E ++A P+ + L+ FLG
Sbjct: 718 DLKSGYWQVGLEPDSIPKTAFITCQGLYEFTVLPFGIKNAAATFQRLMDSVLVNLKGKSCFVYINDIVVYSSTIEQHLGHLEEVFRCLHQAGLTLNLRKCNLLQRSLIFLGHVISGEGICTEPGKVEAIQAFPEPRSIKELQRFLG 863 HSP 2 Score: 64.3142 bits (155), Expect = 2.057e-44 Identity = 79/383 (20.63%), Postives = 149/383 (38.90%), Query Frame = -3
Query: 398 EVVIVYGSRHLTAREMGYYSQEKNYYSIMNLLCILEN----LYGNKFIVRTDHKALTFMNTTKNQLAHNFRRSSQTYQNMILSCSIENEMIMEMQMDFHG*KMSFAHNAKQSIKMQRRKIKSEVYKQFTRKCKYCGVSKNQNEDII----FKEIIPLLKGEK-ETVYDEVKQCKYFILIEQLCILSIIEDNKI----LVLVPQNYVTTLINYIHIELC--HLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIE----QGRTRELLLPRPGEKFSEQMVIDIAYMETSEWNKKYVIVIIDRFSKLVSLTGTTEQDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAER 1489
E V+ Y SR L E Y + EK ++ + +E L G F V TDH AL+++ + + + + I DF + + + +I + + C+ G S D +E+ L+ ++ ET E ++ + + + + + + ++ V+VP + + Y H HLG +KTL + + YW ++ + V C IC K K GR + + PG + + + S ++++VI+D SK V + E + +++ + R+G P +++DRG F ++G+ Q+ + Y Q+N L ER
Sbjct: 947 EHVVAYASRLLQGAERNYSTAEKECLAV---VWAVEKWRVYLEGRHFTVITDHSALSWV-----------------FNHPKPTSRLTRWAIRLQTFDF---SVQYRKGKCNIVPDTLSRIPDRMTEGVMAPCQVTGSSDGLPVDWAEIARAQEVDGTLQPQRDETGNQETRKDRIHFVTKNDILFRAVPNQQLGHTLQVVVPVQHREAFLQYAHDNPLSGHLGQMKTLLRLLNIAYWPSIRRDVWTYCKSCEICQKYKPRISKLSGRLQSTPVVEPGYMLGVDL---MGPLPKSPRQNEHLLVIVDYCSKWVEMFPLREAKTSQIVQILIKDIFTRWGTPAYLVSDRGAQFTSRLLHATCRQWGVVQKLTTAYHPQTN-LTER 1302 HSP 3 Score: 48.1358 bits (113), Expect = 2.057e-44 Identity = 18/41 (43.90%), Postives = 30/41 (73.17%), Query Frame = -3
Query: 2153 SPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMPDMH 2275
SP SP+V+V +K+G +R C+D+RR+N+ T ++PMP +
Sbjct: 662 SPWASPVVLVPKKEGGVRFCVDYRRMNSKTHLDAYPMPQVQ 702 HSP 4 Score: 42.3578 bits (98), Expect = 2.057e-44 Identity = 26/73 (35.62%), Postives = 36/73 (49.32%), Query Frame = -2
Query: 1494 WVDKLLSTFY*RLFTLYAET*RNNKQIIWNEECKNSFNSLKKVLCEAPVLEFPMSDRMFIIYTDAS---FGAI 1703
W + ++ F R L A + N IW +EC+ +F +K+ L APVL P F I TDAS GA+
Sbjct: 867 WYHRFITHFSERAAILNALK-KKNAPWIWTQECQKAFEDIKQALITAPVLTPPNFSEPFQIQTDASDQGLGAV 938 HSP 5 Score: 33.113 bits (74), Expect = 2.057e-44 Identity = 31/132 (23.48%), Postives = 53/132 (40.15%), Query Frame = -2
Query: 6 KENNWCELLPRIEFCLNAKIQSSTGFSTFDIVYSRKIN-----LISNSGN-------VQELKDEILKSAKVNIENAGNSMKGSDQDKRGTRILDVGEKVLVRLKPHKRKKNGML------YEGPYEILRFIS 347
K W + +P F +N+ Q STGF+ ++ RK+ L+S N V + + ++ + N++ A +R G+ V V P R G ++GP +I+R IS
Sbjct: 1318 KHRLWDQWIPEFRFAINSAWQESTGFTPAEVALGRKLKGPLERLLSQPPNPDHDAYKVVQRQQDLFCQIRDNVDKAQAKQAKFYNRRRKQVNFVGGDLVWVETHPLSRADKGFAAKLAEKWKGPAKIIRKIS 1449
BLAST of SMED30024572 vs. Ensembl Cavefish
Match: ENSAMXT00000038088.1 (pep primary_assembly:Astyanax_mexicanus-2.0:23:13869500:13874588:-1 gene:ENSAMXG00000037864.1 transcript:ENSAMXT00000038088.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 80.4925 bits (197), Expect = 6.826e-43 Identity = 49/146 (33.56%), Postives = 81/146 (55.48%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*AL---IFKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTIK---VYIQR*KIELVK---APKCVSRLRSFLG 2109
DL Y+QV + + Q KTAF+T + F R+PF L APATFQRL L + + + VYLD+I+I + H + + +VF + + GLK+ ++K + ++ + F+GH + V+ K+ V+ P + +R+FLG
Sbjct: 427 DLASGYWQVGVHPDDQPKTAFATPLGLYHFRRMPFGLCNAPATFQRLMQHCLRGQVAEFLFVYLDDIIIHSADFSLHVQHLEQVFERLSRYGLKLRSDKCKLFRRQVKFLGHVVNKQGVHPDPEKVAAVQEWATPTTIREVRAFLG 572 HSP 2 Score: 66.6254 bits (161), Expect = 6.826e-43 Identity = 99/480 (20.62%), Postives = 174/480 (36.25%), Query Frame = -3
Query: 398 FGLTNYYRRFIKDYSPYMLKHRGIISR*FGMKNVKIVLIV*RKFYVKLQYLNFQCQT--ECL*FTLM--PVL----------------------VLST-----EVVIVYGSRHLTAREMGYYSQEKNYYSI-MNLLCI--------LENLYGNKFIVRTDHKALTFMNTTKNQLAHNFRRSSQTYQNMILSCSIENEMIMEMQMDFHG*KMSFAHNAKQSIKMQRRKIKSEVYKQFTRKCKYCGVSKNQNEDIIFKEIIPLLKGEKETVYDEVKQCKYF----ILIEQLCILSIIEDNKILVLVPQNYVTTLINYIHIELCHLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTRELLLPRPGEKFSEQMVIDIAYMETSEWNKKYVIVIIDRFSKLVSLTGTTEQDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAER 1705
GL YYRRFI ++ + ++ + + V RK + +CQ ECL L+ P+L VLS E VI Y SR L E + NY S + LL + + L G+KF+V TD+ + + + K + + + E+ E M G A + I +R + + ++ + T + + TV ++Q F L+ +L + + + ++ P+ + + H H G+ KTL +++ FYW + + V + C C +K +G + L P E + +D + S + ++V ID F++ T +Q T + + I FG P +DRG FE + ++ +G+ + + Y NG ER
Sbjct: 571 LGLAGYYRRFIDGFAKLARP----------LNSLLVGVPVNRKSSHRGINWTPECQNAFECLKSCLLQAPILAYADFSAPFIVYTDASSQGLGAVLSQMQDGRERVIAYASRSLHPSE----KNDANYSSFKLELLALKWAVTEKFRDYLMGSKFMVMTDNNPVAHLQSAKLGATEQRFPCVRVPPGANVVTAELQEVTAEQDMAIGGVDWQQAQEQDEEICTVKRYVDAGIFPKVTER-----------------------RALPRTVQQLLRQRSRFQSKDGLLHRLILDPCTNEPHLQIVCPKRWQKEVWERYHEATGHAGVEKTLLRIRNHFYWPQMEEKVRGFHSGCVSCGLQK-GKGDPKAPLHPINVSFPLEVLALDFLSLGRSNDTYQNILVAIDMFTRYAWAIPTRDQTAQTTVKALWSHVIQTFGCPLRFHSDRGPNFESALFQQLCEVYGITRSRTTSYHPAGNGRVER 1012 HSP 3 Score: 55.8398 bits (133), Expect = 6.826e-43 Identity = 23/43 (53.49%), Postives = 34/43 (79.07%), Query Frame = -3
Query: 2162 VSKCRSPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMP 2290
+S+ SP +P+V+V +KDGS R C+D+R+LNAVT K +FP+P
Sbjct: 366 ISESSSPWAAPVVMVRKKDGSWRFCVDYRKLNAVTHKDAFPLP 408 HSP 4 Score: 27.335 bits (59), Expect = 6.826e-43 Identity = 10/38 (26.32%), Postives = 20/38 (52.63%), Query Frame = -2
Query: 234 KENNWCELLPRIEFCLNAKIQSSTGFSTFDIVYSRKIN 347
K++ W LP + N + S+TGF+ +++ R +
Sbjct: 1028 KQDRWPAFLPELLQAYNNTVHSATGFTPSYLMFGRVVR 1065 HSP 5 Score: 26.5646 bits (57), Expect = 6.826e-43 Identity = 12/39 (30.77%), Postives = 21/39 (53.85%), Query Frame = -2
Query: 2394 LNFEKKSFFGQIISEFRNIFMASRFDLGYTKIIQHEIKT 2510
L +++ Q++ ++ ++F S D G T IQH I T
Sbjct: 294 LTTSQQAVLSQLLHKWSSVFSQSEEDFGCTDAIQHCIPT 332
BLAST of SMED30024572 vs. Ensembl Cavefish
Match: ENSAMXT00000052892.1 (pep primary_assembly:Astyanax_mexicanus-2.0:15:1687614:1691096:-1 gene:ENSAMXG00000041369.1 transcript:ENSAMXT00000052892.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 89.3521 bits (220), Expect = 5.870e-38 Identity = 55/146 (37.67%), Postives = 83/146 (56.85%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*A---LIFKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTIK---VYIQR*KIELV---KAPKCVSRLRSFLG 2109
DL YYQ+ +SEE + KTAF F F R+P ++ APATFQRL A + V+VYLD+I++ ++ EEH+ + +V +++ GLKI+ +K QF + E ++GH + + K+ V K P + LRSFLG
Sbjct: 165 DLRSGYYQIAMSEEDKEKTAFICPLGFFQFERMPQGITGAPATFQRLMEKAVGDMHLLQVIVYLDDIIVFGKSLEEHEERLLKVLDRLEEVGLKISIDKCQFCQPEVKYVGHVVSKDGISTDPDKVSAVTQWKQPTDLKSLRSFLG 310 HSP 2 Score: 46.595 bits (109), Expect = 5.870e-38 Identity = 19/43 (44.19%), Postives = 31/43 (72.09%), Query Frame = -3
Query: 2168 VSKCRSPKNSPLVIVGQKDGSIRICLDFRRLN--AVTDKYSFP 2290
+ + RSP SP+VI +K+GS+R+C+D+R LN + D+Y+ P
Sbjct: 104 IKESRSPYASPIVIARKKNGSVRMCIDYRTLNNRTIPDQYTMP 146 HSP 3 Score: 35.8094 bits (81), Expect = 5.870e-38 Identity = 14/42 (33.33%), Postives = 23/42 (54.76%), Query Frame = -2
Query: 1494 WNEECKNSFNSLKKVLCEAPVLEFPMSDRMFIIYTDASFGAI 1619
W++ C +F+ + L APVL F R +I++ DAS +
Sbjct: 364 WDKTCTEAFHRIIHCLTHAPVLAFADPSRPYILHVDASMNGL 405 HSP 4 Score: 32.7278 bits (73), Expect = 5.870e-38 Identity = 23/86 (26.74%), Postives = 40/86 (46.51%), Query Frame = -3
Query: 1238 IVYGSRHLTAREMGYYSQEKNYYSIMNLLC--ILENLYGNKFIVRTDHKALTFMNTTK--NQLAHNFRRSSQTYQ-NMILSCSIEN 1480
+ + SR L+ E Y + + ++ + + LYG F VRTD+ LT++ TT N H + + TY ++ C +N
Sbjct: 420 VAFASRKLSTSEQRYPVHQLEFLALKWAVVDKFHDYLYGACFTVRTDNNPLTYILTTAKLNATGHRWLAALATYNFSLQYRCGTQN 505 HSP 5 Score: 28.8758 bits (63), Expect = 5.870e-38 Identity = 19/80 (23.75%), Postives = 40/80 (50.00%), Query Frame = -2
Query: 2367 IYNIDSSLLEVQHTDEEREKVALRILNFEK-------KSFFGQIISEFRNIFMASRFDLGYTKIIQHEIKTVGSPIFRTR 2585
+Y+ D+ ++ Q +D +K+ + +F + K+ ++E N+F + +D+G K ++H I+ S FR R
Sbjct: 1 MYSTDTVVV-AQKSDGRPDKLDPALFDFGQSPIPEIWKNRLRNKLAERPNVFSINEWDVGLAKGVEHHIRLSDSRPFRER 79 HSP 6 Score: 25.0238 bits (53), Expect = 5.870e-38 Identity = 9/15 (60.00%), Postives = 10/15 (66.67%), Query Frame = -3
Query: 1661 FGLTNYYRRFIKDYS 1705
G YYRRFI +YS
Sbjct: 309 LGFCGYYRRFIANYS 323 HSP 7 Score: 75.0998 bits (183), Expect = 1.090e-13 Identity = 45/184 (24.46%), Postives = 83/184 (45.11%), Query Frame = -3
Query: 398 VLVPQNYVTTLINYIHIELCHLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTRELLLPRPGEKFSEQMVIDIAYM-----ETSEWNKKYVIVIIDRFSKLVSLTGTTEQDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAER 934
+++P+ + ++ +H E HLG+ +T +KD FYW +S + I C C K R L + + +D+ M E V+V+ D +++ T +Q +TV V+ + + +G P I +D+G+ FE +E + G+R+ +PY+ Q + ER
Sbjct: 649 LVLPKTFRNQVLKSLHDECGHLGVDRTTQLVKDRFYWPRMSVEIEHYIKTCGRCVAAKTLPQRASPL------NEITSTGPLDLVCMDFLSIEPDSKGIANVLVVTDHYTRYAQAFCTKDQKAVTVAKVLCEKYFVHYGLPARIHSDQGRDFESRLIKELLSMLGIRKSRTTPYRPQGDPQPER 826 HSP 8 Score: 22.7126 bits (47), Expect = 1.090e-13 Identity = 7/39 (17.95%), Postives = 20/39 (51.28%), Query Frame = -2
Query: 231 KENNWCELLPRIEFCLNAKIQSSTGFSTFDIVYSRKINL 347
++ W + + ++ N +TG+S + +++ R+ L
Sbjct: 842 RKQQWSQQISQLVHAYNCTKNDATGYSPYFLMFGREARL 880
BLAST of SMED30024572 vs. Ensembl Cavefish
Match: ENSAMXT00000053730.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02002271.1:240468:243660:-1 gene:ENSAMXG00000043631.1 transcript:ENSAMXT00000053730.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 88.5817 bits (218), Expect = 8.217e-38 Identity = 53/146 (36.30%), Postives = 83/146 (56.85%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*A---LIFKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTIK---VYIQR*KIELVKA---PKCVSRLRSFLG 2109
DL YYQVE+ EE + KTAF T + FNRLP ++ AP+TFQR+ + K V+V+LD++++ + T EEH+ + V + +K GLK++ EK F++ ++GH + V KI + P+ + L+SFLG
Sbjct: 347 DLKSGYYQVEVEEEDKHKTAFVTPMGFWEFNRLPQGVTNAPSTFQRVMEKCMGTMNLKEVLVFLDDLIVFSDTLEEHESRLMRVLTKLKDFGLKLSPEKCHFFRRSVRYLGHIVSEDGVETDPEKISALTTWPKPRNIRELKSFLG 492 HSP 2 Score: 44.669 bits (104), Expect = 8.217e-38 Identity = 19/55 (34.55%), Postives = 37/55 (67.27%), Query Frame = -3
Query: 2156 KLTESKNYQKVSKCRSPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMPDM 2320
L E ++ + + S SP+V+V +K G+IR+C+D+R+LN+ T K ++ +P++
Sbjct: 276 HLKELQDANIIRESESSFASPVVVVKKKSGAIRLCVDYRKLNSQTIKDAYALPNI 330 HSP 3 Score: 39.6614 bits (91), Expect = 8.217e-38 Identity = 16/42 (38.10%), Postives = 26/42 (61.90%), Query Frame = -2
Query: 1494 WNEECKNSFNSLKKVLCEAPVLEFPMSDRMFIIYTDASFGAI 1619
WNE+C +FN+L + L AP+L F ++++TDAS +
Sbjct: 545 WNEKCDEAFNTLIEKLTTAPILGFANPHLPYVLHTDASVQGL 586 HSP 4 Score: 31.9574 bits (71), Expect = 8.217e-38 Identity = 19/67 (28.36%), Postives = 33/67 (49.25%), Query Frame = -3
Query: 1289 VIVYGSRHLTAREMGYYSQEKNYYSIMNLLC--ILENLYGNKFIVRTDHKALTFMNTTKNQLAHNFR 1483
VI Y SR L+ E Y + + + ++ + + LYG +F + TD+ LT++ T+ A R
Sbjct: 600 VIAYASRGLSYSEKRYPTHKLEFLAVKWAVTDKFFDYLYGAEFTIMTDNNPLTYVLTSAKLDAAGHR 666 HSP 5 Score: 31.5722 bits (70), Expect = 8.217e-38 Identity = 11/16 (68.75%), Postives = 12/16 (75.00%), Query Frame = -3
Query: 1661 IFGLTNYYRRFIKDYS 1708
G T YYRRF+KDYS
Sbjct: 490 FLGFTGYYRRFVKDYS 505 HSP 6 Score: 21.9422 bits (45), Expect = 8.217e-38 Identity = 8/31 (25.81%), Postives = 13/31 (41.94%), Query Frame = -2
Query: 2364 IFMASRFDLGYTKIIQHEIKTVGSPIFRTRV 2456
+F D G+T + H I+ F+ R
Sbjct: 232 VFANGDLDYGHTTAVTHRIRLSDPTPFKQRA 262
BLAST of SMED30024572 vs. Ensembl Medaka
Match: ENSORLT00000032005.1 (pep primary_assembly:ASM223467v1:7:31190450:31194463:1 gene:ENSORLG00000029990.1 transcript:ENSORLT00000032005.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 82.8037 bits (203), Expect = 2.341e-48 Identity = 56/149 (37.58%), Postives = 83/149 (55.70%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*ALIF-----KGVVVYLDNILI*AQTQEEH-DRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTIK---VYIQR*KIELV---KAPKCVSRLRSFLG 2109
DL Y QV ++E + KTAF T F +NR+PF L AP TFQRL IF + V++YLD+I++ + T +EH +R+ V ++++ L++ K F++ E ++GH I V KIE V + P VS LR+FLG
Sbjct: 403 DLASGYNQVPVTEGDRAKTAFCTPFGLFEWNRMPFGLCNAPGTFQRLMQ--RIFGDQQCQSVLLYLDDIVVFSSTIDEHLERL---VLGRLQQEKLRVKLPKCAFFQQEVRYLGHVISDQGVSTDPHKIEAVAGWQPPSTVSELRTFLG 546 HSP 2 Score: 78.9518 bits (193), Expect = 2.341e-48 Identity = 109/518 (21.04%), Postives = 192/518 (37.07%), Query Frame = -3
Query: 398 FGLTNYYRRFIKDYSPYMLKHRGIISR*FGMKNVKIVLIV*RKFYVKLQYLNFQCQTECL*FTLMPVL----------------------VLSTEV-----VIVYGSRHLTA--REMGYYSQEKNYYSIMNLLC---ILENLYGNKFIVRTDHKALTFMNTTK------------------------------NQLAHNFRRSSQTYQNMILSCSIENEMIMEMQMDFHG*KMSFAHNAKQSIKMQRRKIKSEVYKQFTRKCKYCGVSKNQNEDIIFKEIIPLLKGEKETVYDE-----------VKQCKYFILIEQLCILSIIEDNK----ILVLVPQNYVTTLINYIHIELCHLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTR-----ELLLPRPGEKFSEQMVIDIAYMETSEWNKKYVIVIIDRFSKLVSLTGTTEQDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAER 1705
G +YYRRF++ ++ ++ G K+ + + + NF + L T PVL VLS E I Y SR L A R M YS K + + E L G+ +V TD+ L++++T K + L+ + SS NM S+ ++ ++Q+ + AH + + V+ Q T V Q D + ++P + ++ Y E ++Q K ++ L ++ + +L+P+ + ++ +H H GI +TL ++ YW +S+ V C C K + R+ L+ RP +E + +D +E S + V+V+ D FSK T +Q TV V++ W +FG P + +D+G+ FE ++ +G+ + +PY NG ER
Sbjct: 545 LGFASYYRRFVEGFARLAAPLHRLVGELDGTKSRRRKASSLQGHWTTECQQNFDALKQKL--TSAPVLAYADFTLPFILEVDASHNGLGAVLSQEQGGSVRPIAYASRGLKATERNMQNYSSMKLEFLALKWAMTEKFREYLLGHHCVVFTDNNPLSYLSTAKLGAMEQRWVAQLAAFDYEIKYRSGRVNRNADALSRHPNHSSAEVGNMAPGSSLPRDL-QQVQV-----QPVLAHCEMEQLM--------PVFPQRT----TAEVQDLQVSDPVLASVLPFWRDKRYPNYSEREGLSKVALTLLRQWKNLAEVDGLVYRRVLMPDSGQEVFQLLLPEILIPEVLEQVHQHHGHQGIERTLALLRARCYWPGMSKDVAHWCQACERCQLAK-DNSRSHSAPLGHLIASRP----NELVAMDFTILEPSRTGVENVLVLTDVFSKYTVAIPTRDQRAATVAKVLVAEWFSKFGVPARLHSDQGRSFESQLIQQLRGLYGIEKSRTTPYHPAGNGQCER 1037 HSP 3 Score: 50.447 bits (119), Expect = 2.341e-48 Identity = 19/45 (42.22%), Postives = 34/45 (75.56%), Query Frame = -3
Query: 2156 VSKCRSPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMPDM 2290
+ + SP SP+V+ +KDGS+R+C+D+R+LN+ T + +FP+P +
Sbjct: 342 IRESNSPYASPIVLARKKDGSLRMCVDYRQLNSKTRRDAFPLPPI 386 HSP 4 Score: 31.5722 bits (70), Expect = 2.341e-48 Identity = 16/58 (27.59%), Postives = 28/58 (48.28%), Query Frame = -2
Query: 2367 EEREKVALRILNFEKKSFFGQIISEFRNIFMASRFDLGYTKIIQHEIKTVGSPIFRTR 2540
E+ + + L L E + ++ ++ N+F A DLG T ++ HEI + R R
Sbjct: 260 EQIDALPLETLPAEDRGKARALLQKYANVFAAHEGDLGCTTLMTHEIPLLDDAPVRQR 317 HSP 5 Score: 31.187 bits (69), Expect = 2.341e-48 Identity = 24/107 (22.43%), Postives = 47/107 (43.93%), Query Frame = -2
Query: 78 KENNWCELLPRIEFCLNAKIQSSTGFSTFDIVYSRKINLISN----------SGNVQELKDEILKSAKVNIENAGNSM------KGSDQDKRGTRI-LDVGEKVLVR 347
++ +W LP++ FC N +TG S F +++ ++ L + +G + E +E +V + A + + + + DK LD G+ VL+R
Sbjct: 1053 RKRDWHSCLPQVTFCYNTTPHQTTGESPFFLMFGQQPRLPIDFMLGQVKEPVNGTIHEWVEEHQARLRVAFDVAKDRLAEAAARRKRNHDKHVQEAPLDEGQLVLLR 1159
BLAST of SMED30024572 vs. Ensembl Medaka
Match: ENSORLT00000041674.1 (pep primary_assembly:ASM223467v1:5:30088905:30092924:1 gene:ENSORLG00000022054.1 transcript:ENSORLT00000041674.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 82.4185 bits (202), Expect = 2.936e-48 Identity = 54/148 (36.49%), Postives = 80/148 (54.05%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*ALIF-----KGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTIK---VYIQR*KIELV---KAPKCVSRLRSFLG 2109
DL Y QV ++E + KTAF F +NR+PF L AP TFQRL IF + V++YLD+I++ + T +EH + V ++++ L++ K F++ E ++GH I V KIE V + P VS LR+FLG
Sbjct: 403 DLASGYNQVPVTEGDRAKTAFCIPFGLFEWNRMPFGLCNAPGTFQRLMQ--RIFGDQQCQSVLLYLDDIVVFSSTIDEHLERLELVLGRLQQEKLRVKLPKCAFFQQEVRYLGHVISDQGVSTDPHKIEAVAGWQPPSTVSELRTFLG 548 HSP 2 Score: 78.9518 bits (193), Expect = 2.936e-48 Identity = 109/518 (21.04%), Postives = 192/518 (37.07%), Query Frame = -3
Query: 398 FGLTNYYRRFIKDYSPYMLKHRGIISR*FGMKNVKIVLIV*RKFYVKLQYLNFQCQTECL*FTLMPVL----------------------VLSTEV-----VIVYGSRHLTA--REMGYYSQEKNYYSIMNLLC---ILENLYGNKFIVRTDHKALTFMNTTK------------------------------NQLAHNFRRSSQTYQNMILSCSIENEMIMEMQMDFHG*KMSFAHNAKQSIKMQRRKIKSEVYKQFTRKCKYCGVSKNQNEDIIFKEIIPLLKGEKETVYDE-----------VKQCKYFILIEQLCILSIIEDNK----ILVLVPQNYVTTLINYIHIELCHLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTR-----ELLLPRPGEKFSEQMVIDIAYMETSEWNKKYVIVIIDRFSKLVSLTGTTEQDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAER 1705
G +YYRRF++ ++ ++ G K+ + + + NF + L T PVL VLS E I Y SR L A R M YS K + + E L G+ +V TD+ L++++T K + L+ + SS NM S+ ++ ++Q+ + AH + + V+ Q T V Q D + ++P + ++ Y E ++Q K ++ L ++ + +L+P+ + ++ +H H GI +TL ++ YW +S+ V C C K + R+ L+ RP +E + +D +E S + V+V+ D FSK T +Q TV V++ W +FG P + +D+G+ FE ++ +G+ + +PY NG ER
Sbjct: 547 LGFASYYRRFVEGFARLAAPLHRLVGELDGTKSRRRKASSLQGHWTTECQQNFDALKQKL--TSAPVLAYADFTLPFILEVDASHNGLGAVLSQEQGGSVRPIAYASRGLKATERNMQNYSSMKLEFLALKWAMTEKFREYLLGHHCVVFTDNNPLSYLSTAKLGAMEQRWVAQLAAFDYEIKYRSGRVNRNADALSRHPNHSSAEVGNMAPGSSLPRDL-QQVQV-----QPVLAHCEMEQLM--------PVFPQRT----TAEVQDLQVSDPVLASVLPFWRDQRYPNYSEREGLSKVALTLLRQWKNLAEVDGLVYRRVLMPDSGQEVFQLLLPEILIPEVLEQVHQHHGHQGIERTLALLRARCYWPGMSKDVAHWCQACERCQLAK-DNSRSHSAPLGHLIASRP----NELVAMDFTILEPSRTGVENVLVLTDVFSKYTVAIPTRDQRAATVAKVLVAEWFSKFGVPARLHSDQGRSFESQLIQQLCGLYGIEKSRTTPYHPAGNGQCER 1039 HSP 3 Score: 50.447 bits (119), Expect = 2.936e-48 Identity = 19/43 (44.19%), Postives = 33/43 (76.74%), Query Frame = -3
Query: 2162 VSKCRSPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMP 2290
+ + SP SP+V+ +KDGS+R+C+D+R+LN+ T + +FP+P
Sbjct: 342 IRESNSPYASPIVLARKKDGSLRMCVDYRQLNSKTRRDAFPLP 384 HSP 4 Score: 31.9574 bits (71), Expect = 2.936e-48 Identity = 16/58 (27.59%), Postives = 28/58 (48.28%), Query Frame = -2
Query: 2367 EEREKVALRILNFEKKSFFGQIISEFRNIFMASRFDLGYTKIIQHEIKTVGSPIFRTR 2540
E+ + + L L E + ++ ++ N+F A DLG T ++ HEI + R R
Sbjct: 260 EQIDALPLETLPAEDRGKAQALLQKYANVFAAHEGDLGCTTLMTHEIPLLDDAPVRQR 317 HSP 5 Score: 31.187 bits (69), Expect = 2.936e-48 Identity = 24/107 (22.43%), Postives = 47/107 (43.93%), Query Frame = -2
Query: 78 KENNWCELLPRIEFCLNAKIQSSTGFSTFDIVYSRKINLISN----------SGNVQELKDEILKSAKVNIENAGNSM------KGSDQDKRGTRI-LDVGEKVLVR 347
++ +W LP++ FC N +TG S F +++ ++ L + +G + E +E +V + A + + + + DK LD G+ VL+R
Sbjct: 1055 RKRDWHSCLPQVTFCYNTTPHQTTGESPFFLMFGQQPRLPIDFMLGQVKEPVNGTIHEWVEEHQARLRVAFDVAKDRLAEAAARRKRNHDKHVQEAPLDEGQLVLLR 1161
BLAST of SMED30024572 vs. Ensembl Medaka
Match: ENSORLT00000027908.1 (uncharacterized protein K02A2.6-like [Source:NCBI gene;Acc:111946802]) HSP 1 Score: 87.4261 bits (215), Expect = 3.018e-46 Identity = 88/389 (22.62%), Postives = 159/389 (40.87%), Query Frame = -3
Query: 398 TEVVIVYGSRHLTAREMGYYSQEKNYYSIMNLLCILE------NLYGNKFIVRTDHKALTFMNTTKNQLAHNFRRS-------SQTYQNMILSCSIENEMIMEMQMDFHG*KMSFAHNAKQSIKMQRRKIKSEVYKQFTRKCKYCG------VSKNQNEDIIFKEIIPLLKGEKETVYDEVKQCKYFILIEQLCILSIIEDNKILVLVPQNYVTTLINYIHIELCHLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAK----RKIEQGRTRELLLPRPGEKFSEQMVIDI-AYMETSEWNKKYVIVIIDRFSKLVSLTGTTEQDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAER 1492
TE + + SR L++ E Y + EK L C+ L+G KF +RTDH+ALT + TTK R + S TY + + + ++ + + + + S K+F C V Q K + P+L+ YF + +L + + ++VP + +TL+ H H G+++T ++D +++ + V+ + ECS+C + K+ Q + + LP E E++ +DI E++ W+ K+ I + D +SK + + TV + L R+G P+ ++TD G F + ++ G++ +S Y SNG ER
Sbjct: 756 TERTVAFASRTLSSTERKYSTVEKEA-----LACVWAVEKWRTYLWGRKFTLRTDHQALTTLLTTKGADRAGMRIARWSACLLSFTYDVVYRAGPLNQTADCLSRLPLPAASFPPEDSEPELVALLATTPVSVTAKEFEDASSSCPELSSLRVQVQQRWPRSAKSVSPVLQ-------------PYFKIRHELSVQDSLVFRGSRLVVPISLRSTLVALAHEG--HQGVVRTKQRLRDLYWFPGMDALVLSQVTECSLCQQTDKSAKVFQAPLQPVPLP---EGPWEKLGLDIVGPFESATWDCKFAITLTDYYSKWPEVAFAPSVNSETVIQI-LSGLFSRYGNPECLVTDNGPQFTSASFSSFLRDKGIKHTRSSVYHPSSNGAVER 1120 HSP 2 Score: 82.8037 bits (203), Expect = 3.018e-46 Identity = 54/148 (36.49%), Postives = 78/148 (52.70%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*ALIFKGVV---VYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTIKV--------YIQR*KIELVKAPKCVSRLRSFLG 2109
DL AY+QV L +S+ TAF T E F F R+P+ L++AP+ FQ+L A + GV YLD+I+ +T EHD + V +K GL++N +K F ++ F+GH + ++Q I AP LRSFLG
Sbjct: 529 DLESAYHQVLLHPDSRDLTAFITHEGLFRFCRVPYGLASAPSAFQKLM--ATVLHGVPNVQFYLDDIICYGRTAHEHDAALETVRQKLKTAGLQLNDKKCHFRQTSLKFLGHLVTAEGIKPDTEHLQ--AITQAPAPSDAVSLRSFLG 672 HSP 3 Score: 41.2022 bits (95), Expect = 3.018e-46 Identity = 18/40 (45.00%), Postives = 25/40 (62.50%), Query Frame = -3
Query: 2156 SPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMPDM 2275
SP SP+V+ +K+G IR+C+D R N SFP+P M
Sbjct: 473 SPWVSPIVVTQRKNGKIRMCVDLREPNKAIVVDSFPLPHM 512 HSP 4 Score: 36.965 bits (84), Expect = 3.018e-46 Identity = 19/52 (36.54%), Postives = 28/52 (53.85%), Query Frame = -2
Query: 1494 RNNKQIIWNEECKNSFNSLKKVLCEAPVLEFPMSDRMFIIYTDAS---FGAI 1640
R Q +W EE + F ++K++L E+P L + II TDAS GA+
Sbjct: 696 RQGTQFVWTEEAQRCFLTVKRLLLESPALALFNPELPTIISTDASDYGVGAV 747
BLAST of SMED30024572 vs. Ensembl Medaka
Match: ENSORLT00000045836.1 (pep primary_assembly:ASM223467v1:15:13736160:13740855:-1 gene:ENSORLG00000022583.1 transcript:ENSORLT00000045836.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 85.8853 bits (211), Expect = 8.159e-46 Identity = 88/389 (22.62%), Postives = 159/389 (40.87%), Query Frame = -3
Query: 398 TEVVIVYGSRHLTAREMGYYSQEKNYYSIMNLLCILE------NLYGNKFIVRTDHKALTFMNTTKNQLAHNFRRS-------SQTYQNMILSCSIENEMIMEMQMDFHG*KMSFAHNAKQSIKMQRRKIKSEVYKQFTRKCKYCG------VSKNQNEDIIFKEIIPLLKGEKETVYDEVKQCKYFILIEQLCILSIIEDNKILVLVPQNYVTTLINYIHIELCHLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAK----RKIEQGRTRELLLPRPGEKFSEQMVIDI-AYMETSEWNKKYVIVIIDRFSKLVSLTGTTEQDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAER 1492
TE + + SR L++ E Y + EK L C+ L+G KF +RTDH+ALT + TTK R + S TY + + + ++ + + + + S K+F C V Q K + P+L+ YF + +L + + ++VP + +TL+ H H G+++T ++D +++ + V+ + ECS+C + K+ Q + + LP E E++ +DI E++ W+ K+ I + D +SK + + TV + L R+G P+ ++TD G F + ++ G++ +S Y SNG ER
Sbjct: 756 TERTVAFASRTLSSTERKYSTVEKEA-----LACVWAVEKWRTYLWGRKFTLRTDHQALTTLLTTKGADRAGMRIARWSARLLSFTYDVVYRAGPLNQTADCLSRLPLPAASFPPEDSEPELVALLATTPVSVTAKEFEDASSSCPELSSLRVQVQQWWPRSAKSVSPVLQ-------------PYFKIRHELSVQDSLVFRGSRLVVPISLRSTLVALAHEG--HQGVVRTKQRLRDLYWFPGMDALVLSQVTECSLCQQTDKSAKVFQAPLQPVPLP---EGPWEKLGLDIVGPFESATWDCKFAITLTDYYSKWPEVAFAPSVNSETVIQI-LSGLFSRYGNPECLVTDNGPQFTSASFSSFLRDKGIKHTRSSVYHPSSNGAVER 1120 HSP 2 Score: 82.8037 bits (203), Expect = 8.159e-46 Identity = 54/148 (36.49%), Postives = 78/148 (52.70%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*ALIFKGVV---VYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTIKV--------YIQR*KIELVKAPKCVSRLRSFLG 2109
DL AY+QV L +S+ TAF T E F F R+P+ L++AP+ FQ+L A + GV YLD+I+ +T EHD + V +K GL++N +K F ++ F+GH + ++Q I AP LRSFLG
Sbjct: 529 DLESAYHQVLLHPDSRDLTAFITHEGLFRFCRVPYGLASAPSAFQKLM--ATVLHGVPNVQFYLDDIICYGRTAHEHDAALETVRQKLKTAGLQLNDKKCHFRQTSLKFLGHLVTAEGIKPDTEHLQ--AITQAPAPSDAVSLRSFLG 672 HSP 3 Score: 41.2022 bits (95), Expect = 8.159e-46 Identity = 18/40 (45.00%), Postives = 25/40 (62.50%), Query Frame = -3
Query: 2156 SPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMPDM 2275
SP SP+V+ +K+G IR+C+D R N SFP+P M
Sbjct: 473 SPWVSPIVVTQRKNGKIRMCVDLREPNKAIVVDSFPLPHM 512 HSP 4 Score: 36.965 bits (84), Expect = 8.159e-46 Identity = 19/52 (36.54%), Postives = 28/52 (53.85%), Query Frame = -2
Query: 1494 RNNKQIIWNEECKNSFNSLKKVLCEAPVLEFPMSDRMFIIYTDAS---FGAI 1640
R Q +W EE + F ++K++L E+P L + II TDAS GA+
Sbjct: 696 RQGTQFVWTEEAQRCFLTVKRLLLESPALALFNPELPTIISTDASDYGVGAV 747
BLAST of SMED30024572 vs. Ensembl Medaka
Match: ENSORLT00000044147.1 (uncharacterized protein K02A2.6-like [Source:NCBI gene;Acc:111947635]) HSP 1 Score: 95.9005 bits (237), Expect = 3.099e-42 Identity = 56/146 (38.36%), Postives = 82/146 (56.16%), Query Frame = -1
Query: 1699 DLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*ALI-FKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTIKV--------YIQR*KIELVKAPKCVSRLRSFLG 2109
DL AY+QV L+EES+ TAF T E F F R+P+ L +AP+ F +L L KGV YLD++++ + Q+EHDR + V + + + GL++N EK F + F+GH I +++ I AP + LRSFLG
Sbjct: 528 DLANAYHQVLLAEESRDITAFITHEGLFRFRRVPYGLCSAPSAFSKLMSLVLKGLKGVQNYLDDVIVYGRDQQEHDRNLQAVLAALNEAGLQLNMEKCHFNQKSLQFLGHVISAKGLLPNEDHLK--AIAEAPAPGDATTLRSFLG 671 HSP 2 Score: 80.1073 bits (196), Expect = 3.099e-42 Identity = 113/484 (23.35%), Postives = 187/484 (38.64%), Query Frame = -3
Query: 398 FGLTNYYRRFIKDYSPYMLKHRGII---------SR*FGMKNVKIVLIV*RKFYVKLQYLNFQCQTECL*FTLMPVLVL----STEVVIVYGSRHLTAREMGYYSQEKNYYSIMNLLCILEN------LYGNKFIVRTDHKALTFMNTTKN------QLAH------------NFRRSSQTYQNMILS----CSIEN-EMIMEMQMDFHG*KMSFAHNAKQSIKMQRRKIKSEVYKQFTRKCKYCGVSKNQNEDIIFKEIIPLLKGEKETVYDEVKQCK-YFILIEQLCILSIIE-DNKILVLVPQNYVTTLINYIHIELCHLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTRELLLPRPGEKFSEQMV-IDI-AYMETSEWNKKYVIVIIDRFSKL--VSLTGTTEQDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAER 1705
GLT +Y +F+K+Y+ + R + VK +I V L T+ + L +L TE + + SR LT E Y EK L C+ L+G +F +RTDH+ALT + TK ++A +R +Q Y LS S+EN E ++E ++ + + ++K+ K FT C+ C E +E + +G +T + YF + ++L + +I LVP +IN H H G+++T ++D ++W + V + I C C L P P Q V IDI +++ + +Y I +ID ++K V+ T + IT F L RFG P +++D G F + +++ ++ S Y Q+NG ER
Sbjct: 670 LGLTAWYAKFVKNYASLVEPMRACLHTDTFKWTSEAQASFDAVKTRIINSPALAVFEPSLPTIVATDASDYGLGAILTQLHPDKTERTVAFASRSLTPAERKYSIVEKE-----ALACVWAVEKWRTFLWGTRFTLRTDHQALTTLLATKGLGRAGMRIARWSARLLCFTYDVEYRAGAQNYAADCLSRLPLPSVENAEPVVEPEL----------------VALLDTELKALTVKDFTAACQVC------PELTALREQ--MKQGWPKTAQSLPAMLQPYFAVRDELSVEEVIVYRGPYRRLVPVAVRKQIINLAH--ETHQGVVRTKQRLRDLYWWPYMDLCVEETIKACVTCQLNDKSAKIHPAPLHPIPFPDAPWQKVGIDIVGPFDSAAGDCRYAITLIDHYTKWPEVAFTPSITTSAITTF---LSTIFSRFGNPTELISDNGTQFTSTEFADYLAVRDIKHRKVSLYYPQANGAIER 1119 HSP 3 Score: 38.5058 bits (88), Expect = 3.099e-42 Identity = 16/40 (40.00%), Postives = 25/40 (62.50%), Query Frame = -3
Query: 2156 SPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMPDM 2275
SP SP+V+ +K+G +R+C+D+R N S P+P M
Sbjct: 472 SPWVSPIVVTLRKNGKVRMCVDYREPNKAIVMDSHPLPHM 511
BLAST of SMED30024572 vs. Planmine SMEST
Match: SMESG000081257.1 (SMESG000081257.1) HSP 1 Score: 306.99 bits (785), Expect = 1.730e-156 Identity = 191/452 (42.26%), Postives = 256/452 (56.64%), Query Frame = -3
Query: 410 FGLTNYYRRFIKDYSPYMLKHRGIISR*FGMKNVKIVLIV*RKFYVKLQYLNFQCQTECL*FTLMPVLVLSTEVVIVYGSRHLTAREMGYYSQEKNYYSIMNLLCILEN-LYGNKFIVRTDHKALTFMNTTKNQLAHNFR---------------RSSQTYQNMILSCSIENEMIMEMQMDFHG*KMSFAHNAKQSIKMQRRKIKSEVYKQFTRKCKYCGVSKNQNEDIIFKEIIPLLKG-EKETVYDEVKQC--KYFILIE-QLCILSIIEDNKILVLVPQNYVTTLINYIHIELCHLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTRELLLPRPGEKFSEQMVIDIAYMETSEWNKKYVIVIIDRFSKLVSLTGTTEQDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNG 1705
GLT YYRRFIK+YS + G + + RK ++ L+ L C+ L + L + HLT E+GY K + + LYG +F+ RTDHKALTFMNTTK ++ F+ R + N + N + QM+ H + K + R I S K + + QNED + +II L G E Y + KY +++ Q +L I D K+ V+VP +YV +L+NY HIELCHLGI KTL+Y+KDFF+ +++Q + +CIN+C ICA RKI+QGRT+E+LLPR GE+F EQ+V+DI YMET E +KKY+IVIID FSKLVSL QDE T+ NV+L+NWIYRFG+P+SILTDR +IFEGS R+WM KFG++QEF+SPYQHQSNG
Sbjct: 686 LGLTTYYRRFIKNYSMIAAPLYAATT---GCDKMIVWTEECRKRFINLKKL--LCEAPILEYPRADRLFIIDTDDSFEAIGHLTKHEIGYCVTRKELLVLHEFIVHFRQYLYGRRFVARTDHKALTFMNTTKKPISPQFQTWMANFSEYDFALQYRKGNEHGNAGGWSRLNNTICSHYQME---------HKDAKKAKCRTRCINSLQGSSNIMKI----IKQKQNEDKVTSQIISHLNGNEAHISYKTISSSIFKYLKILQIQDNVLMINTDGKLAVVVPDSYVKSLVNYFHIELCHLGINKTLFYLKDFFFLPSMNQIITECINKCKICASRKIDQGRTKEILLPRTGERFLEQIVVDIDYMETKE-SKKYMIVIIDCFSKLVSLP----QDEATILNVILNNWIYRFGRPESILTDRERIFEGSMFRDWMEKFGIKQEFSSPYQHQSNG 1114 HSP 2 Score: 192.971 bits (489), Expect = 1.730e-156 Identity = 119/244 (48.77%), Postives = 151/244 (61.89%), Query Frame = -1
Query: 1699 NPRRQPMHLEIKIDELVRNLLKAKIIRKCQSVEAQRILHWS*LGRKMVLFVYVLILEGL----------MQ*QINIHSQCQICXXXXXXXXXXXXXXIDLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH---*ALIFKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTI-KVYIQR*K-----IELVKAPKCVSRLRSFLG 2373
NPR QPMHLE K++ELV+NLL+A ++RKCQS ++ + + + +L + MQ ++ + +I F IDLGQAYY VEL+E SQIKTAFST+E QFCFNRLPF LSTAPATFQ+L H L+FKGVVVYLD+ILI + QE HD+++FEVF+ I+ GLK+N EK F KS NFIGHT+ + IQ K IE PK + LRSFLG
Sbjct: 456 NPRGQPMHLEAKVEELVQNLLEAGVVRKCQSPWNTPLVIVGKPDGSVRICLDFRLLNSVTEKFSFPMPDMQLLLDCLGKSKI------------FYSIDLGQAYYLVELNENSQIKTAFSTKEGQFCFNRLPFGLSTAPATFQKLMHQILEGLVFKGVVVYLDDILIYGENQETHDKLLFEVFTRIRDSGLKVNPEKCAFNKSVLNFIGHTVSEKGIQTNKRKISEIENATEPKSTTELRSFLG 687 HSP 3 Score: 97.0561 bits (240), Expect = 1.730e-156 Identity = 50/102 (49.02%), Postives = 68/102 (66.67%), Query Frame = -2
Query: 2373 RN*DFVVARNISSGFIGGVDMIMLIGLIFKEIYNIDSSLLEVQHTDEEREKVALRILNFEKKSFFGQIISEFRNIFMASRFDLGYTKIIQHEIKTVGSPIFR 2678
R + ++ R I FIGGVD + + G+ KE+ NI+SSL+ + TD +R K L L +K S G +IS+F NIFMASRFDLG+TK+I HE+KT G PI +
Sbjct: 354 RELNVIIVRKIHPNFIGGVDTMKIFGMELKEVNNIESSLVNKRFTDSDRLKNTLLTLKLDKNSELGTLISQFSNIFMASRFDLGHTKVITHELKTSGPPILQ 455 HSP 4 Score: 172.555 bits (436), Expect = 3.997e-43 Identity = 101/246 (41.06%), Postives = 153/246 (62.20%), Query Frame = -2
Query: 2775 VKQELNERFSTFELKVKKMLDDIFDCGISTEKIVGSIASNHVNNDRLK*NLLSKPDMSREEMRITAKNFESRDLAGIKQIINEIQAVEKRTYSNVVQNKSQQLLDNS-PKKVFTQNNRYFNSSYQKKPLQKERQLHSSRPIVE--HPRTEIIDDGADKGYKHSVSLKKISRKLYK*CCGLPEPKDKILKRGQCFCCDENDHLRFQCPLKDKYLFCCKSDNLFRDCYLLXXXXXXXX---XRVFCIH 3494
++Q +ERF TFE++VKK++D+++DCGIS E IV + + V NDRLK NL +K +++REE+R AK E+R+ K+ E+Q V K Y+NV Q+K +Q + S +++ NR + SYQ + + ERQ +P+ + + RT + ++ +S+K I+RK+Y CGLPEP ++ LK G+CFCC +NDH+RF+CPLKDK L C K + FR LL + + R V+CIH
Sbjct: 1 MRQNHDERFVTFEIRVKKLVDEVYDCGISKENIVKLVLCHGVRNDRLKGNLSTKDNITREEIRNLAKVHEAREGYNTKERSEEVQVVVKSIYANVTQDKQKQWIQKSFNNRIYP--NRQTDISYQNRNERNERQFDKVKPVTKQYNERTTM-----ERRPPPLISMKHITRKIYNRFCGLPEPVEERLKPGKCFCCGQNDHMRFECPLKDKCLICGKEGHSFRCFNLLVKSRSNTQRLERSVYCIH 239 HSP 5 Score: 81.6481 bits (200), Expect = 3.412e-15 Identity = 34/46 (73.91%), Postives = 41/46 (89.13%), Query Frame = -3
Query: 2153 VSKCRSPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMPDMH 2290
V KC+SP N+PLVIVG+ DGS+RICLDFR LN+VT+K+SFPMPDM
Sbjct: 481 VRKCQSPWNTPLVIVGKPDGSVRICLDFRLLNSVTEKFSFPMPDMQ 526
BLAST of SMED30024572 vs. Planmine SMEST
Match: SMESG000038955.1 (SMESG000038955.1) HSP 1 Score: 173.326 bits (438), Expect = 5.215e-102 Identity = 103/199 (51.76%), Postives = 138/199 (69.35%), Query Frame = -1
Query: 1780 RRQPMHLEIKIDELVRNLLKAKIIRKCQSVEAQRILHWS*LGRKMVLFVYVLILEGLMQ*QINIHSQCQICXXXXXXXXXXXXXXIDLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH---*ALIFKGVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHT 2367
+++PMHLE K++ELV+NLL+A ++RK S ++ L + + + +L + + + + +S + +++ KIFS IDLGQAYYQVEL+E SQIKTAFST+E QFCFNRLPF LSTAPATFQ+L H L+FKGVVVYLD ILI + QE HD+++FEVF+ I+ GLK+N EK F KS NFIGHT
Sbjct: 147 KKKPMHLEGKVEELVQNLLEAGVVRKSISPWNTPLVIVGKLDGSIRMCLDFRLLNSVTE-KFSFYSP-DMKLLLDCLGNSKIFSSIDLGQAYYQVELNENSQIKTAFSTKEGQFCFNRLPFGLSTAPATFQKLMHQILEGLVFKGVVVYLDEILIYGENQEIHDKLLFEVFTRIRDSGLKVNPEKCAFNKSVLNFIGHT 343 HSP 2 Score: 154.066 bits (388), Expect = 5.215e-102 Identity = 85/173 (49.13%), Postives = 110/173 (63.58%), Query Frame = -2
Query: 1176 LYAET*RNNKQIIWNEECKNSFNSLKKVLCEAPVLEFPMSDRMFIIYTDASFGAIDRSC-----DCIWI------KTFDST*NGILFTRKELLLNNEFIVHFRESLW-Q*IHCENRSQSIDFHEHYKKPISPQFQT*LANLSEYDFELQYRK*DDHGNADGFSWIKNEFCS*C 1658
+Y T N+K I+W EEC+NSF +LKK+LCEAP+LE+P +DR+F+I TDASFGAI DC + + G T KELL +EFIVHFR+ L+ + +++ F KKPISPQFQT +ANLSE+DF LQYRK ++HGNADG S + N CS C
Sbjct: 352 MYVATTGNDKMIVWTEECRNSFINLKKLLCEAPILEYPRADRLFVIDTDASFGAIGAVLSQIKEDCTEVVIAYGSRHLTKHEMGHCVTIKELLALHEFIVHFRQYLYGRRFVARTDHKALTFMNTTKKPISPQFQTWMANLSEHDFALQYRKGEEHGNADGKSRLNNTKCSHC 524 HSP 3 Score: 73.9442 bits (180), Expect = 5.215e-102 Identity = 36/65 (55.38%), Postives = 47/65 (72.31%), Query Frame = -2
Query: 2397 KEIYNIDSSLLEVQHTDEEREKVALRILNFEKKSFFGQIISEFRNIFMASRFDLGYTKIIQHEIK 2591
KE+ NI+S L+ + TD +R K+ L L +K S G +IS+F NIFMASRFDLG+TK+I HEIK
Sbjct: 83 KEVNNIESLLVNKKFTDSDRLKITLSTLKLDKNSKLGTLISKFSNIFMASRFDLGHTKVITHEIK 147 HSP 4 Score: 34.2686 bits (77), Expect = 5.215e-102 Identity = 17/33 (51.52%), Postives = 24/33 (72.73%), Query Frame = -1
Query: 1084 IMQNKA*RCKEEKSKVRYINSLQGNANIVESAK 1182
+M+NK KE K + RYINSLQG++NI++ K
Sbjct: 525 LMENKD--AKEAKCRTRYINSLQGSSNIMKIIK 555 HSP 5 Score: 90.1225 bits (222), Expect = 1.505e-34 Identity = 46/91 (50.55%), Postives = 58/91 (63.74%), Query Frame = -2
Query: 105 MITTSLKGGFKENNWCELLPRIEFCLNAKIQSSTGFSTFDIVYSRKINLISNSGNVQELKDEILKSAKVNIENAGNSMKGSDQDKRGTRIL 377
M+ TSL +NNWC LLPRIEF NA IQ+ST FS F+IVY RKINL S ++Q+ ++EI K N+ A +M+ D D GTR L
Sbjct: 667 MLATSLAEIKTKNNWCRLLPRIEFSFNATIQNSTKFSPFEIVYGRKINLYSGVEHIQKFREEIEDETKTNLVKAATTMQNRDIDNLGTRSL 757 HSP 6 Score: 77.7962 bits (190), Expect = 1.505e-34 Identity = 38/66 (57.58%), Postives = 47/66 (71.21%), Query Frame = -3
Query: 392 QDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAERTI 589
QD++ + N + P+SILTDRG IFEGS R+WM KFG++QEF+SPYQHQSN LAER I
Sbjct: 596 QDDVLMINTDGKLAVVVPDSPESILTDRGIIFEGSMFRDWMEKFGIKQEFSSPYQHQSNILAERII 661 HSP 7 Score: 66.6254 bits (161), Expect = 1.166e-10 Identity = 31/45 (68.89%), Postives = 37/45 (82.22%), Query Frame = -3
Query: 2156 VSKCRSPKNSPLVIVGQKDGSIRICLDFRRLNAVTDKYSFPMPDM 2290
V K SP N+PLVIVG+ DGSIR+CLDFR LN+VT+K+SF PDM
Sbjct: 170 VRKSISPWNTPLVIVGKLDGSIRMCLDFRLLNSVTEKFSFYSPDM 214
BLAST of SMED30024572 vs. Planmine SMEST
Match: SMESG000057289.1 (SMESG000057289.1) HSP 1 Score: 257.299 bits (656), Expect = 4.945e-98 Identity = 119/186 (63.98%), Postives = 153/186 (82.26%), Query Frame = -3
Query: 392 DNKILVLVPQNYVTTLINYIHIELCHLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTRELLLPRPGEKFSEQMVIDIAYMETSEWNKKYVIVIIDRFSKLVSLTGTTEQDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAERTI 949
D K+ V+VP +Y+ +L+NY +IELCHLGI KTL+Y+KDFF+W +++Q + + IN+C ICA RKI QG+T+E+LLPR GE+F EQ+V+DIAYMET E N KY+IVIID FSKLVSL+ + QDE T+ N +L+NWIYRFG+P+SILTD G+ FEGS R+WM KF ++QEF SPYQHQSNGLAER I
Sbjct: 5 DGKLAVVVPDSYIKSLVNYFYIELCHLGIKKTLFYLKDFFFWPSMNQIIAEFINKCKICASRKIYQGQTKEILLPRTGERFLEQIVVDIAYMETKESN-KYMIVIIDSFSKLVSLSVASTQDEATILNFILNNWIYRFGRPESILTDIGRTFEGSMFRDWMEKFDIKQEFCSPYQHQSNGLAERNI 189 HSP 2 Score: 122.479 bits (306), Expect = 4.945e-98 Identity = 61/113 (53.98%), Postives = 77/113 (68.14%), Query Frame = -2
Query: 39 MITTSLKGGFKENNWCELLPRIEFCLNAKIQSSTGFSTFDIVYSRKINLISNSGNVQELKDEILKSAKVNIENAGNSMKGSDQDKRGTRILDVGEKVLVRLKPHKRKKNGMLY 377
M+ TSL +NNWC LLP+IEF LNA IQ+ST FS F+IVY RKINL S G++Q+ ++EI K N+ A SM+ D D GTR+ V EKVLVRL+P +RKK G+ Y
Sbjct: 195 MLATSLTEIKTKNNWCILLPKIEFSLNATIQNSTKFSPFEIVYCRKINLYSGVGHIQKCREEIEDETKTNLVKAATSMQNRDLDSLGTRVFMVEEKVLVRLEPQRRKKEGIQY 307
BLAST of SMED30024572 vs. Planmine SMEST
Match: SMESG000029259.1 (SMESG000029259.1) HSP 1 Score: 152.525 bits (384), Expect = 1.065e-90 Identity = 100/234 (42.74%), Postives = 137/234 (58.55%), Query Frame = -1
Query: 1699 PRRQPMHLEIKIDELVRNLLKAKIIRKCQSV-EAQRILHWS*LGRKMVLFVYVLILEGLMQ*QINIHSQCQICXXXXXXXXXXXXXXIDLGQAYYQVELSEESQIKTAFSTREWQFCFNRLPF*LSTAPATFQRLTH*AL--IFK-GVVVYLDNILI*AQTQEEHDRIIFEVFSGIKKDGLKINTEKYQFYKSEFNFIGHTIK---VYIQR*KIELVKA---PKCVSRLRSFLG 2370
PRRQP++LE KI+E ++NL IIRKC S + W + + L + L + + Q + I+ + FS IDLG AYYQV+L +ESQ KTAFST+E QFCFNR+PF ++ AP TFQ L L ++K GV+VYLD+ILI +T+E+H I +V I GL++N EK Q ++ E F+GH I + KIE +++ PKCV LRSFLG
Sbjct: 2732 PRRQPINLEDKIEEAIKNLENNGIIRKCNSPWNTPLVCVWKKEKKDIRLCLDFRQLNKITERQA--FPMPNVDEILDILHGSRYFSSIDLGNAYYQVKLDKESQEKTAFSTKEGQFCFNRMPFGIAAAPGTFQELMTKVLKDLWKDGVMVYLDDILIFTKTEEDHYNIFGKVLGKIATAGLRLNPEKCQIFRKEVKFLGHIINKDGIQTDNTKIEAIQSFQKPKCVKNLRSFLG 2963 HSP 2 Score: 136.346 bits (342), Expect = 1.065e-90 Identity = 141/472 (29.87%), Postives = 206/472 (43.64%), Query Frame = -3
Query: 398 FGLTNYYRRFIKDYSPYMLKHRGIIS----------------R*FG-MKNVKIVLIV*RKFYVKLQYLNFQ----CQTECL*FTLMPVLVL----STEVVIVYGSRHLTAREMGYYSQEKNYYSIMNLLCILEN--LYGNKFIVRTDHKALTFMNTTKNQLAHNFRRSSQTYQNMILSCSIENEM---IMEMQMDFHG*KMSFAHNAKQSIKMQRRKIKSEVYKQFTRKCKYCGVSKNQNEDIIFKEIIPLLKGEKETVYDEVKQCKYFILIEQLCILSIIEDNKIL-----VLVPQNYVTTLINYIHIELCHLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTRELLLPRPGEKFSEQMVIDIAYMETSEWNKK-YVIVIIDRFSKLVSLTGTTEQDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAER 1705
G+ NYYRRFIKDY+ K R + S + FG MK I V L + +F+ T+ T+ VL E VI YGS +++ E GY K +I C N LYG +F++RTDHKA+TFM TTK + F QT+ N + S I+ E D K Q + ++ + G +K QN+++ +EI L E K CK+ I+E+N +L + +P + +I +H+ LCH G K Y+++ NL+ V K I C C K K +T+E + E++ +DI +NKK Y+ IID +SK +SLT +QDE T+ +L+ WI +FG P + D GK FE +E G+ F+SPY H +NG+ ER
Sbjct: 2962 LGICNYYRRFIKDYAK---KARALESICGKNNEKIRWTEMCEKAFGEMKEALITAPV-------LVFPDFRKEFILDTDASFDTIGAVLSQKDEKGHEHVIAYGSHAMSSHEKGYCITRKELLAIY-YFCKHFNHYLYGKRFVLRTDHKAITFMVTTKKPITAQF----QTWINYLSSLDIKMEYRKGTSHTNADMLSRKT--CGTCVQCMMEHEDAKTGKIKTRILTVTAEGGYNKWQNDNMEVQEIKNKL---------ENKDCKF-----------IMENNTVLTKQGKIWIPSDNRQRMIKEVHVLLCHAGAQKVTKYIQNNCDMENLATEVKKVIENCERCQKMKTITTKTKEETQTIKSTEPFEKIYMDICGPLKETFNKKKYICGIIDHYSKYISLTAINKQDERTISETLLNKWILKFGAPKELHVDCGKNFEARSIKELAKTAGIELIFSSPYHHNTNGIIER 3396 HSP 3 Score: 58.151 bits (139), Expect = 1.065e-90 Identity = 35/111 (31.53%), Postives = 58/111 (52.25%), Query Frame = -2
Query: 2376 RN*DFVVARNISSGFIGGVDMIMLIGL----IFKEIYNIDSSLLEVQH------TDEEREKVALRILNFEKKSFFGQIISEFRNIFMASRFDLGYTKIIQHEIKTVGSPIF 2678
RN F++ +N+ IGGV++ L G+ I +E + E++ TDEER + A+ +L +I +N+FMA ++D+G T +I+H+I T G PI
Sbjct: 2619 RNVTFIIVKNLIPEIIGGVELQRLFGIELKCILEEHEKRSDFICEIEARFGRIITDEERLRHAIDVLKVTGNKRLLEIFQANKNVFMADKWDIGCTNLIKHKIITKGEPIM 2729 HSP 4 Score: 50.8322 bits (120), Expect = 1.065e-90 Identity = 36/122 (29.51%), Postives = 67/122 (54.92%), Query Frame = -2
Query: 9 ITTSLKGGFKENNWCELLPRIEFCLNAKIQSSTGFSTFDIVYSRKINLISNSGNVQELKDEILKSAKVNIENAGNSMKGSDQDKRGTRILDVGEKVLVRLKPHKRKKNGMLYEGPYEILRFI 374
I SL G ++N W +++P I++ LNA +Q +TG S +I++ RKI+ + N + ++++ K IE+ K S + R ++ + VL+ K R K+ +EGPY++++ I
Sbjct: 3405 INASLNEGGRKN-WADIVPEIKYTLNATVQKTTGVSPAEIIFGRKIDRMKWYSNKEINREDMEK----RIEDKTLKPKIS----KTVRNFEMEDVVLI--KQEIRNKDDARWEGPYKVIKKI 3515 HSP 5 Score: 80.8777 bits (198), Expect = 7.337e-15 Identity = 58/157 (36.94%), Postives = 77/157 (49.04%), Query Frame = -2
Query: 1206 RNNKQIIWNEECKNSFNSLKKVLCEAPVLEFPMSDRMFIIYTDASF---GAIDRSCD--------CIWIKTFDST*NGILFTRKELLLNNEFIVHFRESLW-Q*IHCENRSQSIDFHEHYKKPISPQFQT*LANLSEYDFELQYRK*DDHGNADGFS 1640
+NN++I W E C+ +F +K+ L APVL FP + FI+ TDASF GA+ D S G TRKELL F HF L+ + ++I F KKPI+ QFQT + LS D +++YRK H NAD S
Sbjct: 2988 KNNEKIRWTEMCEKAFGEMKEALITAPVLVFPDFRKEFILDTDASFDTIGAVLSQKDEKGHEHVIAYGSHAMSSHEKGYCITRKELLAIYYFCKHFNHYLYGKRFVLRTDHKAITFMVTTKKPITAQFQTWINYLSSLDIKMEYRKGTSHTNADMLS 3144 HSP 6 Score: 55.8398 bits (133), Expect = 2.902e-7 Identity = 24/46 (52.17%), Postives = 36/46 (78.26%), Query Frame = -3
Query: 2156 VSKCRSPKNSPLVIVGQKDG-SIRICLDFRRLNAVTDKYSFPMPDM 2290
+ KC SP N+PLV V +K+ IR+CLDFR+LN +T++ +FPMP++
Sbjct: 2756 IRKCNSPWNTPLVCVWKKEKKDIRLCLDFRQLNKITERQAFPMPNV 2801
BLAST of SMED30024572 vs. Planmine SMEST
Match: SMESG000073986.1 (SMESG000073986.1) HSP 1 Score: 219.935 bits (559), Expect = 1.823e-84 Identity = 144/343 (41.98%), Postives = 191/343 (55.69%), Query Frame = -3
Query: 392 YGNKFIVRTDHKALTFMNTTKNQLAHNFRRSSQTYQNMILSCSIENEMIMEMQMDFHG*K--MSFAHNAKQS-IKMQRRKIKSEVYKQFTRKCKYCGVSKN--------QNEDIIFKEIIPLLKGEKETVYDEVKQCKYF----ILIEQLCILSIIEDNKILVLVPQNYVTTLINYIHIELCHLGILKTLYYMKDFFYWNNLSQSVIKCINECSICAKRKIEQGRTRELLLPRPGEKFSEQMVIDIAYMETSEWNKKYVIVIIDRFSKLVSLTGTTEQDEITVFNVVLDNWIYRFGKPDSILTDRGKIFEGS*RREWM*KFGLRQEFNSPYQHQSNGLAERTI 1375
YG +F+ +TDHKALTFMNTTK + F Q + + S + + + + HG MS +N S +M+R+ S+ K TR S N QN+D + II L G + + E F IL Q +L I D K+ V+VP +Y +L CI +C I A RKI+QGR +E+LLPR ++F EQ+V+DIAYMET E +K +IVII+RFSKLVSL+ + QDE T+ NV+L+NWIYRFG+P+SILTDRG+IFEGS +WM KFG++QEF+SPYQHQSNGLAER I
Sbjct: 166 YGRRFVAKTDHKALTFMNTTKKPINPQF-------QTWMANLSEYDFALQYRKAEEHGNADGMSRLNNTICSHCQMERKD--SKRAKCRTRYINSLQGSSNIIKIIKQKQNKDRVTSVIILHLNGNEAHISYETISSSIFKYSKILQIQDDVLMINTDGKLAVVVPDSYAKSL-----------------------------------CIKKCKIYASRKIDQGRAKEILLPRTRKRFLEQIVVDIAYMETKE-SKNCMIVIINRFSKLVSLSAASTQDEATILNVILNNWIYRFGRPESILTDRGRIFEGSMFHDWMEKFGIKQEFSSPYQHQSNGLAERII 463 HSP 2 Score: 114.775 bits (286), Expect = 1.823e-84 Identity = 55/105 (52.38%), Postives = 73/105 (69.52%), Query Frame = -2
Query: 63 MITTSLKGGFKENNWCELLPRIEFCLNAKIQSSTGFSTFDIVYSRKINLISNSGNVQELKDEILKSAKVNIENAGNSMKGSDQDKRGTRILDVGEKVLVRLKPHK 377
M+ TSL +NNWC LLP+IEF LNA I+ ST FS F+I+Y RKINL S G++Q+ ++EI K N+ A +MK D D +GTR+ VG+KVLVRL+PH+
Sbjct: 469 MLATSLAKIKTKNNWCILLPKIEFSLNATIKISTKFSPFEIIYWRKINLYSGVGHIQKFREEIEDETKTNLVKAATTMKNRDLDNQGTRVFMVGKKVLVRLEPHR 573 HSP 3 Score: 76.2554 bits (186), Expect = 9.729e-14 Identity = 34/56 (60.71%), Postives = 42/56 (75.00%), Query Frame = -2
Query: 1176 QSIDFHEHYKKPISPQFQT*LANLSEYDFELQYRK*DDHGNADGFSWIKNEFCS*C 1343
+++ F KKPI+PQFQT +ANLSEYDF LQYRK ++HGNADG S + N CS C
Sbjct: 177 KALTFMNTTKKPINPQFQTWMANLSEYDFALQYRKAEEHGNADGMSRLNNTICSHC 232 HSP 4 Score: 62.3882 bits (150), Expect = 1.762e-9 Identity = 36/83 (43.37%), Postives = 51/83 (61.45%), Query Frame = -2
Query: 2430 RN*DFVVARNISSGFIGGVDMIMLIGLIFKEIYNIDSSLLEVQHTDEEREKVALRILNFEKKSFFGQIISEFRNIFMASRFDL 2678
R + ++ R I F+G V + + G+ KE+ NI+SSL + TD +R K+ L L +K S G +IS+FRNIFMASRFD
Sbjct: 79 REFNVIIVRKIHPDFLGVVGTMKIFGMELKEVINIESSLDNKRFTDSDRLKITLLTLKLDKNSKLGTLISQFRNIFMASRFDF 161 The following BLAST results are available for this feature:
BLAST of SMED30024572 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30024572 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30024572 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30024572 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of SMED30024572 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of SMED30024572 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30024572 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of SMED30024572 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30024572 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of SMED30024572 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30024572 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30024572 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30024572 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of SMED30024572 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30024572 ID=SMED30024572|Name=SMED30024572|organism=Schmidtea mediterranea sexual|type=transcript|length=4230bpback to top protein sequence of SMED30024572-orf-1 >SMED30024572-orf-1 ID=SMED30024572-orf-1|Name=SMED30024572-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=111bp MKEIIGLESLIGFFDLLLTEEANILWRDFKKLGLTDEDARSWFVDTFKSQback to top protein sequence of SMED30024572-orf-2 >SMED30024572-orf-2 ID=SMED30024572-orf-2|Name=SMED30024572-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=105bp MPVLVLSTEVVIVYGSRHLTAREMGYYSQEKNYYSIMNLLCILENLYGNKback to top protein sequence of SMED30024572-orf-3 >SMED30024572-orf-3 ID=SMED30024572-orf-3|Name=SMED30024572-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=125bp MITTSLKGGFKENNWCELLPRIEFCLNAKIQSSTGFSTFDIVYSRKINLIback to top protein sequence of SMED30024572-orf-4 >SMED30024572-orf-4 ID=SMED30024572-orf-4|Name=SMED30024572-orf-4|organism=Schmidtea mediterranea sexual|type=polypeptide|length=238bp MSFAHNAKQSIKMQRRKIKSEVYKQFTRKCKYCGVSKNQNEDIIFKEIIPback to top protein sequence of SMED30024572-orf-5 >SMED30024572-orf-5 ID=SMED30024572-orf-5|Name=SMED30024572-orf-5|organism=Schmidtea mediterranea sexual|type=polypeptide|length=117bp MSREEMRITAKNFESRDLAGIKQIINEIQAVEKRTYSNVVQNKSQQLLDNback to top Annotated Terms
The following terms have been associated with this transcript:
|
