SMED30024529

Overview


Name	SMED30024529
Smed ID	SMED30024529
Length (bp)	601

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30024529 (SMED30024529) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30024529 (SMED30024529) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30024529 (SMED30024529) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30024529 (SMED30024529) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30024529

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
intestinal phagocyte	SMED30024529	SMESG000070890.1	Contig1671	GPL15192	PMID:23079596 Forsthoefel et al., 2012
intestinal phagocyte	SMED30024529	SMESG000004003.1	Contig1671	GPL15192	PMID:23079596 Forsthoefel et al., 2012
neoblast	SMED30024529	SMESG000004003.1	dd_Smed_v4_2482_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018

Note: Hover over icons to view figure legend

Alignments

SMED30024529 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.003193:33621..34332 -	2817

Homology

[Close]

BLAST of SMED30024529 vs. Ensembl Human
Match: TXNDC17 (thioredoxin domain containing 17 [Source:HGNC Symbol;Acc:HGNC:28218])

HSP 1 Score: 73.559 bits (179), Expect = 1.760e-16
Identity = 37/121 (30.58%), Postives = 68/121 (56.20%), Query Frame = -1

Query:  179 EQLYIGNYDKYIGKIDELKSLQNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGMELCS-NLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGNQCTDSNLINEFF 538
            E++ +  ++++   +++      ++  +F GS +++ G SWCPDC  AEP + +G++  S   VF   +VG K  WK   N FR   +LK+T +PTL+      +L+ ++C  +NL+   F
Sbjct:    5 EEVSVSGFEEFHRAVEQHNG--KTIFAYFTGS-KDAGGKSWCPDCVQAEPVVREGLKHISEGCVFIYCQVGEKPYWKDPNNDFR--KNLKVTAVPTLLKYGTPQKLVESECLQANLVEMLF 120

[Close]

BLAST of SMED30024529 vs. Ensembl Human
Match: TXNDC17 (thioredoxin domain containing 17 [Source:HGNC Symbol;Acc:HGNC:28218])

HSP 1 Score: 50.447 bits (119), Expect = 5.491e-8
Identity = 26/76 (34.21%), Postives = 42/76 (55.26%), Query Frame = -1

Query:  179 RDAEPFIEKGMELCS-NLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGNQCTDSNLINEFF 403
             +AEP + +G++  S   VF   +VG K  WK   N FR   +LK+T +PTL+      +L+ ++C  +NL+   F
Sbjct:   22 HNAEPVVREGLKHISEGCVFIYCQVGEKPYWKDPNNDFR--KNLKVTAVPTLLKYGTPQKLVESECLQANLVEMLF 95

[Close]

BLAST of SMED30024529 vs. Ensembl Celegans
Match: Y71H2AR.1 (pep chromosome:WBcel235:III:2834663:2836261:1 gene:WBGene00022188.1 transcript:Y71H2AR.1.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y71H2AR.1)

HSP 1 Score: 63.5438 bits (153), Expect = 4.801e-13
Identity = 40/112 (35.71%), Postives = 58/112 (51.79%), Query Frame = -1

Query:  179 DELKSLQNS--VILFFCGSTEESTGDSWCPDC----RDAEPFIEKGMELCSNLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINL-KKKNRLIGNQCTDSNLINEFF 493
            + LKS+     V+  F GS   +TG+SWCPDC       E  I+       ++ F  V VG ++ W+     FR D  LKLT IPTL+ +  K  RL+  Q  + +L+ +FF
Sbjct:   17 ETLKSIGKGKRVVALFTGSKILTTGESWCPDCVVAEPVVEEVIKDAAVAGLDVHFVTVFVGNREVWRDPAVGFRTDPTLKLTCIPTLLEVGNKAKRLLERQIANKHLVKDFF 128

[Close]

BLAST of SMED30024529 vs. Ensembl Fly
Match: cl (gene:FBgn0000318 transcript:FBtr0329967)

HSP 1 Score: 76.6406 bits (187), Expect = 6.912e-18
Identity = 40/117 (34.19%), Postives = 63/117 (53.85%), Query Frame = -1

Query:  179 IGNYDKYIGKIDELKSLQNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGMELCS-NLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGNQCTDSNLINEFF 526
            +  YD++  K++EL++    V + F G  +E  G+SWCP C  AEP I   ++    N  F  V+VG +  WK     FR D +  L  +PTL+  K+  RL G +C++ +L+   F
Sbjct:    7 VKGYDEFTKKMEELENGDEPVHVLFSGGKDEK-GESWCPYCVKAEPVIHDALKKAPGNSHFVHVDVGERAYWKDLNCPFRKDPNTHLIFLPTLLRWKRPQRLDGERCSNQDLVEMMF 122

[Close]

BLAST of SMED30024529 vs. Ensembl Fly
Match: cl (gene:FBgn0000318 transcript:FBtr0079095)

HSP 1 Score: 76.6406 bits (187), Expect = 6.912e-18
Identity = 40/117 (34.19%), Postives = 63/117 (53.85%), Query Frame = -1

Query:  179 IGNYDKYIGKIDELKSLQNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGMELCS-NLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGNQCTDSNLINEFF 526
            +  YD++  K++EL++    V + F G  +E  G+SWCP C  AEP I   ++    N  F  V+VG +  WK     FR D +  L  +PTL+  K+  RL G +C++ +L+   F
Sbjct:    7 VKGYDEFTKKMEELENGDEPVHVLFSGGKDEK-GESWCPYCVKAEPVIHDALKKAPGNSHFVHVDVGERAYWKDLNCPFRKDPNTHLIFLPTLLRWKRPQRLDGERCSNQDLVEMMF 122

[Close]

BLAST of SMED30024529 vs. Ensembl Fly
Match: cl (gene:FBgn0000318 transcript:FBtr0329968)

HSP 1 Score: 76.6406 bits (187), Expect = 6.912e-18
Identity = 40/117 (34.19%), Postives = 63/117 (53.85%), Query Frame = -1

Query:  179 IGNYDKYIGKIDELKSLQNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGMELCS-NLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGNQCTDSNLINEFF 526
            +  YD++  K++EL++    V + F G  +E  G+SWCP C  AEP I   ++    N  F  V+VG +  WK     FR D +  L  +PTL+  K+  RL G +C++ +L+   F
Sbjct:    7 VKGYDEFTKKMEELENGDEPVHVLFSGGKDEK-GESWCPYCVKAEPVIHDALKKAPGNSHFVHVDVGERAYWKDLNCPFRKDPNTHLIFLPTLLRWKRPQRLDGERCSNQDLVEMMF 122

[Close]

BLAST of SMED30024529 vs. Ensembl Fly
Match: CG3939 (gene:FBgn0040396 transcript:FBtr0343600)

HSP 1 Score: 55.8398 bits (133), Expect = 5.090e-10
Identity = 34/101 (33.66%), Postives = 49/101 (48.51%), Query Frame = -1

Query:  179 QNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGM--ELCSNLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGNQCTDSNLINEFF 475
            Q S I  +    ++  G SWCPDC  AE  I         ++ +  +V+VG ++ W   +N FR      +  IPTLI  K   RL G+Q   S+L+  FF
Sbjct:   23 QRSPIYIYFYGEKDKDGRSWCPDCVAAEETIMSAFRNHAPADCMILVVDVGSRESWIGKDNMFR-KPPYSVEGIPTLIRWKGVERLDGDQLLKSSLLELFF 122

[Close]

BLAST of SMED30024529 vs. Ensembl Fly
Match: CG3939 (gene:FBgn0040396 transcript:FBtr0070508)

HSP 1 Score: 55.8398 bits (133), Expect = 5.090e-10
Identity = 34/101 (33.66%), Postives = 49/101 (48.51%), Query Frame = -1

Query:  179 QNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGM--ELCSNLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGNQCTDSNLINEFF 475
            Q S I  +    ++  G SWCPDC  AE  I         ++ +  +V+VG ++ W   +N FR      +  IPTLI  K   RL G+Q   S+L+  FF
Sbjct:   23 QRSPIYIYFYGEKDKDGRSWCPDCVAAEETIMSAFRNHAPADCMILVVDVGSRESWIGKDNMFR-KPPYSVEGIPTLIRWKGVERLDGDQLLKSSLLELFF 122

[Close]

BLAST of SMED30024529 vs. Ensembl Zebrafish
Match: txndc17 (thioredoxin domain containing 17 [Source:NCBI gene;Acc:445062])

HSP 1 Score: 67.0106 bits (162), Expect = 4.112e-14
Identity = 36/121 (29.75%), Postives = 61/121 (50.41%), Query Frame = -1

Query:  179 EQLYIGNYDKYIGKIDELKSLQNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGM-ELCSNLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGNQCTDSNLINEFF 538
            E++ +  Y+++   + + K     +  +F G  +E  G SWCPDC  AEP +   +  L    VF   +VG +  WK + N F+    LKLT +PTL+      +L+  +C  ++L+   F
Sbjct:    5 EEVAVHGYEEFCKAVSDRKG--KEIFAYFSGDKDEH-GKSWCPDCVKAEPVVRAELPHLPEGTVFIYCQVGDRPYWKDSNNDFK--KTLKLTGVPTLLRYGTPQKLVEEECFKADLVRMMF 120

[Close]

BLAST of SMED30024529 vs. Ensembl Mouse
Match: Txndc17 (thioredoxin domain containing 17 [Source:MGI Symbol;Acc:MGI:1289248])

HSP 1 Score: 71.633 bits (174), Expect = 6.357e-16
Identity = 35/104 (33.65%), Postives = 57/104 (54.81%), Query Frame = -1

Query:  179 LKSLQNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGM-ELCSNLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGNQCTDSNLINEFF 487
            +K  +   I  +   ++++ G SWCPDC +AEP I +G+  +  + VF   +VG K  WK   N FR    LK+T +PTL+      +L+ ++C  S+L+   F
Sbjct:   19 VKEHEGKTIFAYFSGSKDTEGKSWCPDCVEAEPVIREGLKHVTEDCVFIYCQVGDKPYWKDPNNDFR--QKLKITAVPTLLKYGTPQKLVESECCQSSLVEMIF 120

[Close]

BLAST of SMED30024529 vs. UniProt/SwissProt
Match: sp|Q5REA8|TXD17_PONAB (Thioredoxin domain-containing protein 17 OS=Pongo abelii OX=9601 GN=TXNDC17 PE=2 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 3.434e-16
Identity = 38/121 (31.40%), Postives = 68/121 (56.20%), Query Frame = -1

Query:  179 EQLYIGNYDKYIGKIDELKSLQNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGMELCS-NLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGNQCTDSNLINEFF 538
            E++ +  ++++   +++      ++  +F GS +++ G SWCPDC  AEP + +G++  S   VF   +VG K  WK   N FR   +LK+T +PTLI      +L+ ++C  +NL+   F
Sbjct:    5 EEVSVSGFEEFHRAVEQHNG--KTIFAYFTGS-KDAGGKSWCPDCVQAEPVVREGLKHISEGCVFIYCQVGEKPYWKDPNNDFR--KNLKVTAVPTLIKYGTPQKLVESECLQANLVEMLF 120

[Close]

BLAST of SMED30024529 vs. UniProt/SwissProt
Match: sp|Q9BRA2|TXD17_HUMAN (Thioredoxin domain-containing protein 17 OS=Homo sapiens OX=9606 GN=TXNDC17 PE=1 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 8.450e-16
Identity = 37/121 (30.58%), Postives = 68/121 (56.20%), Query Frame = -1

Query:  179 EQLYIGNYDKYIGKIDELKSLQNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGMELCS-NLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGNQCTDSNLINEFF 538
            E++ +  ++++   +++      ++  +F GS +++ G SWCPDC  AEP + +G++  S   VF   +VG K  WK   N FR   +LK+T +PTL+      +L+ ++C  +NL+   F
Sbjct:    5 EEVSVSGFEEFHRAVEQHNG--KTIFAYFTGS-KDAGGKSWCPDCVQAEPVVREGLKHISEGCVFIYCQVGEKPYWKDPNNDFR--KNLKVTAVPTLLKYGTPQKLVESECLQANLVEMLF 120

[Close]

BLAST of SMED30024529 vs. UniProt/SwissProt
Match: sp|Q9CQM5|TXD17_MOUSE (Thioredoxin domain-containing protein 17 OS=Mus musculus OX=10090 GN=Txndc17 PE=1 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 4.447e-15
Identity = 35/104 (33.65%), Postives = 57/104 (54.81%), Query Frame = -1

Query:  179 LKSLQNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGM-ELCSNLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGNQCTDSNLINEFF 487
            +K  +   I  +   ++++ G SWCPDC +AEP I +G+  +  + VF   +VG K  WK   N FR    LK+T +PTL+      +L+ ++C  S+L+   F
Sbjct:   19 VKEHEGKTIFAYFSGSKDTEGKSWCPDCVEAEPVIREGLKHVTEDCVFIYCQVGDKPYWKDPNNDFR--QKLKITAVPTLLKYGTPQKLVESECCQSSLVEMIF 120

[Close]

BLAST of SMED30024529 vs. UniProt/SwissProt
Match: sp|T1SH39|TXD17_EPICO (Thioredoxin domain-containing protein 17 OS=Epinephelus coioides OX=94232 PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 3.786e-14
Identity = 37/121 (30.58%), Postives = 63/121 (52.07%), Query Frame = -1

Query:  179 EQLYIGNYDKYIGKIDELKSLQNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGM-ELCSNLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGNQCTDSNLINEFF 538
            E++ +  YD++   + E K     +  +F G  ++++G SWCPDC  AEP +   M  L    VF   +VG +  WK + N F+    LKL+ +PTL+      +L+  +C  ++L+   F
Sbjct:    5 EEVNVRGYDEFCQAVSERKG--KDIFAYFSGD-KDASGKSWCPDCVTAEPIVRGQMSHLPEGSVFIYCQVGERAYWKDSTNAFK--KTLKLSGVPTLLRYGTPQKLVEEECFKADLVRMMF 120

[Close]

BLAST of SMED30024529 vs. UniProt/SwissProt
Match: sp|Q6DBT3|TXD17_DANRE (Thioredoxin domain-containing protein 17 OS=Danio rerio OX=7955 GN=txndc17 PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 3.104e-13
Identity = 36/121 (29.75%), Postives = 61/121 (50.41%), Query Frame = -1

Query:  179 EQLYIGNYDKYIGKIDELKSLQNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGM-ELCSNLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGNQCTDSNLINEFF 538
            E++ +  Y+++   + + K     +  +F G  +E  G SWCPDC  AEP +   +  L    VF   +VG +  WK + N F+    LKLT +PTL+      +L+  +C  ++L+   F
Sbjct:    5 EEVAVHGYEEFCKAVSDRKG--KEIFAYFSGDKDEH-GKSWCPDCVKAEPVVRAELPHLPEGTVFIYCQVGDRPYWKDSNNDFK--KTLKLTGVPTLLRYGTPQKLVEEECFKADLVRMMF 120

[Close]

BLAST of SMED30024529 vs. TrEMBL
Match: A0A0K2T6Y6 (Thioredoxin domaincontaining protein 17like [Musca domestica] (Fragment) OS=Lepeophtheirus salmonis OX=72036 PE=4 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 1.461e-21
Identity = 45/116 (38.79%), Postives = 70/116 (60.34%), Query Frame = -1

Query:  179 IGNYDKYIGKIDELKSLQNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGMELCSNLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGNQCTDSNLINEFF 526
            +  Y+ ++ K+ E+++L  ++ + F GS + S+G SWCPDC  AEP ++  M+  + + F  V VG +D WK  +  FR + D +L  IPTLI  K +++L G   TDSNLI   F
Sbjct:    5 VHGYEGFLSKVKEMEALGGTLAVLFTGSIDPSSGKSWCPDCVVAEPIVDSVMKDKTEVHFLRVFVGPRDFWKDPQCPFRTNPDTRLKSIPTLIIWKSQSKLDGYDITDSNLIAMLF 120

[Close]

BLAST of SMED30024529 vs. TrEMBL
Match: A0A3M6V4T7 (DUF953 domain-containing protein OS=Pocillopora damicornis OX=46731 GN=pdam_00014191 PE=4 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 1.555e-21
Identity = 49/120 (40.83%), Postives = 75/120 (62.50%), Query Frame = -1

Query:  179 QLYIGNYDKYIGKIDELKSLQNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGMELC-SNLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGNQCTDSNLINEFF 535
            QL +      +  ++E K  + +V+  F G+  E+ G+SWCPDCRDAEP +E  ++   S++VF LV VG K EWKS +N+FR + D KL EIPT+++     RL    C +S ++++FF
Sbjct:    8 QLQVEGLSNLMQTLEEHKGKRRTVM--FIGAMNEN-GESWCPDCRDAEPIVEAALKKAPSDMVFILVIVGDKPEWKSPDNEFRTNPDFKLKEIPTIVDYGTDKRLGCEDCKNSEIVDKFF 124

[Close]

BLAST of SMED30024529 vs. TrEMBL
Match: A0A4S2L3I7 (DUF953 domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_009946 PE=4 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 4.494e-21
Identity = 46/107 (42.99%), Postives = 66/107 (61.68%), Query Frame = -1

Query:  179 QNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGMELCSNL-VFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLIN-------LKKKNRLIGNQCTDSNLINEFF 475
            Q  V  FF G+T+  TGDSWCPDCR AEP +E+  +  S   +F + EVG +D WK+  N FR     KLTEIPTL++       +  ++RL+ + C D +L+++FF
Sbjct:   26 QLRVYAFFIGTTDPITGDSWCPDCRTAEPIVEEATKKMSEKDLFIMCEVGDRDHWKTATNPFRTHPVFKLTEIPTLLSFYLDVGEIAVRSRLVNDGCKDPDLVDKFF 132

[Close]

BLAST of SMED30024529 vs. TrEMBL
Match: D3PHN2 (Thioredoxin domain-containing protein 17 OS=Lepeophtheirus salmonis OX=72036 GN=TXD17 PE=2 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 6.011e-21
Identity = 44/116 (37.93%), Postives = 69/116 (59.48%), Query Frame = -1

Query:  179 IGNYDKYIGKIDELKSLQNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGMELCSNLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGNQCTDSNLINEFF 526
            +  Y+ ++ K+ E+++L  ++ + F GS + S+G SWCPDC  AEP ++  M+  + + F  V VG +D WK  +  FR + D +L  IPTLI  K +++L G   TD NLI   F
Sbjct:    5 VHGYEGFLSKVKEMEALGGTLAVLFTGSIDPSSGKSWCPDCVVAEPIVDSVMKDKTEVHFLRVFVGPRDFWKDPQCPFRTNPDTRLKSIPTLIIWKSQSKLDGYDITDPNLIAMLF 120

[Close]

BLAST of SMED30024529 vs. TrEMBL
Match: A0A4S2L317 (DUF953 domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_009946 PE=4 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 9.323e-21
Identity = 48/107 (44.86%), Postives = 64/107 (59.81%), Query Frame = -1

Query:  179 QNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGMELCSNL-VFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLIN-------LKKKNRLIGNQCTDSNLINEFF 475
            Q  V  FF G+T+  TGDSWCPDCR AEP +E+  +  S   +F + EVG +D WK+  N FR     KLTEIPTL++       +  K+RL+G+ C DS  +  FF
Sbjct:   26 QLRVYAFFIGTTDPITGDSWCPDCRTAEPIVEEATKKMSEKDLFIMCEVGDRDHWKTPTNPFRTHPVFKLTEIPTLLSFCLNSGEIGVKSRLLGDGCKDSAQVGTFF 132

[Close]

BLAST of SMED30024529 vs. Ensembl Cavefish
Match: txndc17 (thioredoxin domain containing 17 [Source:NCBI gene;Acc:103032862])

HSP 1 Score: 65.4698 bits (158), Expect = 1.060e-13
Identity = 36/121 (29.75%), Postives = 60/121 (49.59%), Query Frame = -1

Query:  179 EQLYIGNYDKYIGKIDELKSLQNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGM-ELCSNLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGNQCTDSNLINEFF 538
            E++ +  Y+++   + +       +  +F G  ++  G SWCPDC  AEP +   M  L    VF   +VG +  WK   N+F+    LKLT +PTL+      +L+  QC  ++L+   F
Sbjct:    5 EEVKVRGYEEFCKTVSDRAG--KDIFAYFSGDKDQQ-GQSWCPDCVKAEPVVRGEMNHLPEGSVFIYCQVGDRPYWKDPNNEFK--KTLKLTGVPTLLRYGTPQKLVEEQCFKADLVRMMF 120

[Close]

Query:  179 EQLYIGNYDKYIGKIDELKSLQNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGM-ELCSNLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGNQCTDSNLINEFF 538
            E++ +  Y+++   + +       +  +F G  ++  G SWCPDC  AEP +   M  L    VF   +VG +  WK   N+F+    LKLT +PTL+      +L+  QC  ++L+   F
Sbjct:    5 EEVKVRGYEEFCKTVSDRAG--KDIFAYFSGDKDQQ-GQSWCPDCVKAEPVVRGEMNHLPEGSVFIYCQVGDRPYWKDPNNEFK--KTLKLTGVPTLLRYGTPQKLVEEQCFKADLVRMMF 120

[Close]

BLAST of SMED30024529 vs. Ensembl Sea Lamprey
Match: txndc17 (thioredoxin domain containing 17 [Source:NCBI gene;Acc:445062])

HSP 1 Score: 74.7146 bits (182), Expect = 6.193e-18
Identity = 38/123 (30.89%), Postives = 66/123 (53.66%), Query Frame = -1

Query:  179 SCEQLYIGNYDKYIGKIDELKSLQNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGM-ELCSNLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGNQCTDSNLINEFF 544
            SCE++ +  Y ++   +  L+  +  +   F GS +++ G+SWCPDC  AEP +   +  L    VF   +VG +  WK+ +N+FR    L+LT +PTL+      +L+  +C    L++  F
Sbjct:    3 SCEEVSVSGYAEFTSAVRRLQGRE--IFALFSGS-KDAGGESWCPDCVAAEPVVRSALNTLPEGAVFIYCQVGDRAYWKNKDNEFR--KQLQLTAVPTLLKYGSPQKLVEEECLKPELVSMLF 120

[Close]

BLAST of SMED30024529 vs. Ensembl Nematostella
Match: EDO42121 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2D0])

HSP 1 Score: 78.1814 bits (191), Expect = 6.971e-19
Identity = 48/130 (36.92%), Postives = 65/130 (50.00%), Query Frame = -1

Query:  179 FAMSCEQLYIGNYDKYIGKIDELKSLQNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGMELCSN----LVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGN-QCTDSNLINEFF 553
             A    QL + N  +   KI+E K     + + F G+ +E TGDSWCPDC  AEP +EK +E         VF L  VG +  WK   N+FR    L+L  IPTL+        +G+ QC  + L+  FF
Sbjct:    1 MAGRIPQLQVENCAELFEKIEEQKG--KRIFVMFIGAIDE-TGDSWCPDCVRAEPNVEKAVENFQGDKNAAVFILAIVGDRPTWKDPNNEFRVHEKLRLKSIPTLMEWNTTKEPLGDEQCQKAELVESFF 127

[Close]

BLAST of SMED30024529 vs. Ensembl Medaka
Match: txndc17 (thioredoxin domain containing 17 [Source:NCBI gene;Acc:101163188])

HSP 1 Score: 65.0846 bits (157), Expect = 1.536e-13
Identity = 36/121 (29.75%), Postives = 61/121 (50.41%), Query Frame = -1

Query:  179 EQLYIGNYDKYIGKIDELKSLQNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGM-ELCSNLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGNQCTDSNLINEFF 538
            E++ +  Y+++   + + K     +  +F G T++  G SWCPDC  AEP +   M  L    VF   +VG +  WK   N+F+    LKL+ +PTL+      +L+  +C  + L+   F
Sbjct:    5 EEVNVHGYEEFCKAVSDRKG--KEIFAYFSG-TKDDKGTSWCPDCVTAEPIVRGEMSHLPDGSVFIYCQVGERAYWKDPNNEFK--KTLKLSGVPTLLRYGTPQKLVEEECFKAELVKMLF 120

[Close]

BLAST of SMED30024529 vs. Planmine SMEST
Match: SMESG000004003.1 (SMESG000004003.1)

HSP 1 Score: 214.157 bits (544), Expect = 3.459e-72
Identity = 104/104 (100.00%), Postives = 104/104 (100.00%), Query Frame = -1

Query:  236 MSCEQLYIGNYDKYIGKIDELKSLQNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGMELCSNLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKK 547
            MSCEQLYIGNYDKYIGKIDELKSLQNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGMELCSNLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKK
Sbjct:    1 MSCEQLYIGNYDKYIGKIDELKSLQNSVILFFCGSTEESTGDSWCPDCRDAEPFIEKGMELCSNLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKK 104

[Close]

BLAST of SMED30024529 vs. Planmine SMEST
Match: SMESG000074476.1 (SMESG000074476.1)

HSP 1 Score: 67.781 bits (164), Expect = 1.148e-14
Identity = 38/99 (38.38%), Postives = 52/99 (52.53%), Query Frame = -1

Query:  173 VILFFCGSTEESTGDSWCPDCRDAEPFIEKGME-LCSNLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGNQCTDSNLINEFFLN 466
            + + F G T   TG SWCPDC  A P IEK +E    N +F  V VG ++++K  +  FR    + LT IPTL+      RL   +C   +LI E  +N
Sbjct:   24 IFVLFTGDTNSETGHSWCPDCVLANPVIEKMIEKFPKNAIFITVFVGNREQYKDLDCVFRKHPQITLTGIPTLMEFGTMKRLGPIECLSEDLI-ELLIN 121

[Close]

BLAST of SMED30024529 vs. Planmine SMEST
Match: SMESG000074476.1 (SMESG000074476.1)

HSP 1 Score: 61.6178 bits (148), Expect = 2.469e-12
Identity = 38/102 (37.25%), Postives = 51/102 (50.00%), Query Frame = -1

Query:  173 VILFFCGSTEESTGDSWCPDCRDAEPFIEKGME-LCSNLVFALVEVGYKDE---WKSTENKFRCDSDLKLTEIPTLINLKKKNRLIGNQCTDSNLINEFFLN 466
            + + F G T   TG SWCPDC  A P IEK +E    N +F  V VG ++    +K  +  FR    + LT IPTL+      RL   +C   +LI E  +N
Sbjct:   24 IFVLFTGDTNSETGHSWCPDCVLANPVIEKMIEKFPKNAIFITVFVGNREHDCRYKDLDCVFRKHPQITLTGIPTLMEFGTMKRLGPIECLSEDLI-ELLIN 124

The following BLAST results are available for this feature:

BLAST of SMED30024529 vs. Ensembl Human
BLAST of SMED30024529 vs. Ensembl Celegans
BLAST of SMED30024529 vs. Ensembl Fly
BLAST of SMED30024529 vs. Ensembl Zebrafish
BLAST of SMED30024529 vs. Ensembl Xenopus
BLAST of SMED30024529 vs. Ensembl Mouse
BLAST of SMED30024529 vs. UniProt/SwissProt
BLAST of SMED30024529 vs. TrEMBL
BLAST of SMED30024529 vs. Ensembl Cavefish
BLAST of SMED30024529 vs. Ensembl Sea Lamprey
BLAST of SMED30024529 vs. Ensembl Yeast
BLAST of SMED30024529 vs. Ensembl Nematostella
BLAST of SMED30024529 vs. Ensembl Medaka
BLAST of SMED30024529 vs. Planmine SMEST

BLAST of SMED30024529 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2


Match Name	E-value	Identity	Description
TXNDC17	1.760e-16	30.58	thioredoxin domain containing 17 [Source:HGNC Symb...	[more]
TXNDC17	5.491e-8	34.21	thioredoxin domain containing 17 [Source:HGNC Symb...	[more]

BLAST of SMED30024529 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1


Match Name	E-value	Identity	Description
Y71H2AR.1	4.801e-13	35.71	pep chromosome:WBcel235:III:2834663:2836261:1 gene...	[more]

BLAST of SMED30024529 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
cl	6.912e-18	34.19	gene:FBgn0000318 transcript:FBtr0329967	[more]
cl	6.912e-18	34.19	gene:FBgn0000318 transcript:FBtr0079095	[more]
cl	6.912e-18	34.19	gene:FBgn0000318 transcript:FBtr0329968	[more]
CG3939	5.090e-10	33.66	gene:FBgn0040396 transcript:FBtr0343600	[more]
CG3939	5.090e-10	33.66	gene:FBgn0040396 transcript:FBtr0070508	[more]

BLAST of SMED30024529 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
txndc17	4.112e-14	29.75	thioredoxin domain containing 17 [Source:NCBI gene...	[more]

BLAST of SMED30024529 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30024529 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1


Match Name	E-value	Identity	Description
Txndc17	6.357e-16	33.65	thioredoxin domain containing 17 [Source:MGI Symbo...	[more]

BLAST of SMED30024529 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q5REA8\|TXD17_PONAB	3.434e-16	31.40	Thioredoxin domain-containing protein 17 OS=Pongo ...	[more]
sp\|Q9BRA2\|TXD17_HUMAN	8.450e-16	30.58	Thioredoxin domain-containing protein 17 OS=Homo s...	[more]
sp\|Q9CQM5\|TXD17_MOUSE	4.447e-15	33.65	Thioredoxin domain-containing protein 17 OS=Mus mu...	[more]
sp\|T1SH39\|TXD17_EPICO	3.786e-14	30.58	Thioredoxin domain-containing protein 17 OS=Epinep...	[more]
sp\|Q6DBT3\|TXD17_DANRE	3.104e-13	29.75	Thioredoxin domain-containing protein 17 OS=Danio ...	[more]

BLAST of SMED30024529 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K2T6Y6	1.461e-21	38.79	Thioredoxin domaincontaining protein 17like [Musca...	[more]
A0A3M6V4T7	1.555e-21	40.83	DUF953 domain-containing protein OS=Pocillopora da...	[more]
A0A4S2L3I7	4.494e-21	42.99	DUF953 domain-containing protein OS=Opisthorchis f...	[more]
D3PHN2	6.011e-21	37.93	Thioredoxin domain-containing protein 17 OS=Lepeop...	[more]
A0A4S2L317	9.323e-21	44.86	DUF953 domain-containing protein OS=Opisthorchis f...	[more]

BLAST of SMED30024529 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
txndc17	1.060e-13	29.75	thioredoxin domain containing 17 [Source:NCBI gene...	[more]
txndc17	1.060e-13	29.75	thioredoxin domain containing 17 [Source:NCBI gene...	[more]

BLAST of SMED30024529 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1


Match Name	E-value	Identity	Description
txndc17	6.193e-18	30.89	thioredoxin domain containing 17 [Source:NCBI gene...	[more]

BLAST of SMED30024529 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30024529 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO42121	6.971e-19	36.92	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of SMED30024529 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1


Match Name	E-value	Identity	Description
txndc17	1.536e-13	29.75	thioredoxin domain containing 17 [Source:NCBI gene...	[more]

BLAST of SMED30024529 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3


Match Name	E-value	Identity	Description
SMESG000004003.1	3.459e-72	100.00	SMESG000004003.1	[more]
SMESG000074476.1	1.148e-14	38.38	SMESG000074476.1	[more]
SMESG000074476.1	2.469e-12	37.25	SMESG000074476.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30024529 ID=SMED30024529|Name=SMED30024529|organism=Schmidtea mediterranea sexual|type=transcript|length=601bp
AAATAATGCAAATACAATATAAGTTCAACACAATTTCAATTAAACAGCCT
TTACATAAATAACTCAAAATAAATTTGATCCTAATTATGAAATTTGAAAA
ACAATATCACATAATTAGTAATAGTAATAAACAAATCACCAATTTTATTG
CGTGTCAAAAATCAAATCAAATATTTAGAAAAAACTCATTAATTAAATTT
GAATCCGTGCATTGATTGCCGATTAATCGATTTTTCTTTTTTAAATTTAT
GAGAGTTGGAATTTCAGTTAATTTCAAATCAGAATCGCATCTAAATTTAT
TTTCAGTTGATTTCCATTCGTCTTTATAGCCAACTTCCACCAAAGCAAAT
ACCAAGTTACTACACAATTCCATTCCTTTTTCAATAAAAGGCTCAGCATC
ACGACAATCAGGGCACCAACTATCACCAGTTGATTCTTCAGTAGACCCGC
AGAAAAATAAAATCACCGAATTTTGTAAAGATTTAAGCTCATCAATTTTT
CCTATATATTTGTCATAATTTCCAATATATAATTGTTCACATGACATCGC
AAAAACTTTATTGAGTTTACTCTTAATAAAATTCGGTGATTTTATTTTTC
A

protein sequence of SMED30024529-orf-1

>SMED30024529-orf-1 ID=SMED30024529-orf-1|Name=SMED30024529-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=127bp
MSCEQLYIGNYDKYIGKIDELKSLQNSVILFFCGSTEESTGDSWCPDCRD
AEPFIEKGMELCSNLVFALVEVGYKDEWKSTENKFRCDSDLKLTEIPTLI
NLKKKNRLIGNQCTDSNLINEFFLNI*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000070	intestinal phagocyte

Vocabulary: **INTERPRO**
Term	Definition
IPR036249	Thioredoxin-like_sf
IPR010357	DUF953_thioredox
IPR012336	Thioredoxin-like_fold

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010357	Domain of unknown function DUF953, thioredoxin-like	PFAM	PF06110	DUF953	coord: 26..135 e-value: 1.2E-28 score: 99.1
IPR036249	Thioredoxin-like superfamily	GENE3D	G3DSA:3.40.30.10		coord: 8..137 e-value: 4.2E-33 score: 115.9
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	SSF52833	Thioredoxin-like	coord: 30..132
None	No IPR available	PANTHER	PTHR12452	42-9-9 PROTEIN-RELATED	coord: 21..136

Main menu

SMED30024529

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 3

Owlery

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