SMED30024478

Overview
NameSMED30024478
Smed IDSMED30024478
Length (bp)1435
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30024478 (SMED30024478) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30024478 (SMED30024478) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30024478 (SMED30024478) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30024478 (SMED30024478) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30024478

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30024478SMESG000031417.1 dd_Smed_v4_10312_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30024478SMESG000031417.1 dd_Smed_v4_10312_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30024478SMESG000031417.1 dd_Smed_v4_10312_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30024478SMESG000031417.1 Contig54984newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30024478SMESG000031417.1 Contig54984uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of SMED30024478 vs. Planmine SMEST
Match: SMESG000031417.1 (SMESG000031417.1)

HSP 1 Score: 189.889 bits (481), Expect = 5.553e-56
Identity = 83/86 (96.51%), Postives = 85/86 (98.84%), Query Frame = -2 
Query:  454 MCLVIGKYFIYISWIRCFVDFEFPLLGNDSTVPHPAINCHLVLYILLVVCRGGIRSNSWSTRDAWGFNMHSYSIGYDVHGGEDGVS 711
            MCLVIGKYFIYISW+RCFVDFEFPLLGNDSTVPHPAINCHLVLYILLVVCRGGIRSNSWSTRDA+GFNMHS SIGYDVHGGEDGVS
Sbjct:    1 MCLVIGKYFIYISWVRCFVDFEFPLLGNDSTVPHPAINCHLVLYILLVVCRGGIRSNSWSTRDAYGFNMHSNSIGYDVHGGEDGVS 86
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BLAST of SMED30024478 vs. Planmine SMEST
Match: SMESG000011009.1 (SMESG000011009.1)

HSP 1 Score: 172.94 bits (437), Expect = 8.660e-53
Identity = 82/89 (92.13%), Postives = 86/89 (96.63%), Query Frame = -2 
Query:  445 MCLVIGKYFIYISWIRCFVDFEFPLLGNDSTVPHPAINCHLVLYILLVVCRGGIRSNSWSTRDAWGFNMHSYSIGYDVHGGEDGVSLGA 711
            MCLVIGKYFI+ISWIRCFVDFEFP+LGNDS VPHPAI+CHLVLYILLVVCRG IRSNSWSTRDAWGFNMHSYSIGYD+HGGEDGVSLG 
Sbjct:    1 MCLVIGKYFIFISWIRCFVDFEFPMLGNDSAVPHPAISCHLVLYILLVVCRGCIRSNSWSTRDAWGFNMHSYSIGYDIHGGEDGVSLGG 89
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BLAST of SMED30024478 vs. Planmine SMEST
Match: SMESG000072325.1 (SMESG000072325.1)

HSP 1 Score: 154.451 bits (389), Expect = 1.066e-45
Identity = 72/80 (90.00%), Postives = 76/80 (95.00%), Query Frame = -2 
Query:  472 MCLVIGKYFIYISWIRCFVDFEFPLLGNDSTVPHPAINCHLVLYILLVVCRGGIRSNSWSTRDAWGFNMHSYSIGYDVHG 711
            MCLVIGKYFIY+SWIRCFVD EFP+LGNDS VPHPAINCHLVLYI LVVCR GIRSNSWST+DAWGFN+HSYSIGYDVHG
Sbjct:    1 MCLVIGKYFIYLSWIRCFVDCEFPMLGNDSAVPHPAINCHLVLYIQLVVCRDGIRSNSWSTQDAWGFNIHSYSIGYDVHG 80
[Close]
BLAST of SMED30024478 vs. Planmine SMEST
Match: SMESG000011009.1 (SMESG000011009.1)

HSP 1 Score: 124.405 bits (311), Expect = 1.169e-34
Identity = 59/65 (90.77%), Postives = 62/65 (95.38%), Query Frame = -2 
Query:  445 LLGNDSTVPHPAINCHLVLYILLVVCRGGIRSNSWSTRDAWGFNMHSYSIGYDVHGGEDGVSLGA 639
            +LGNDS VPHPAI+CHLVLYILLVVCRG IRSNSWSTRDAWGFNMHSYSIGYD+HGGEDGVSLG 
Sbjct:    1 MLGNDSAVPHPAISCHLVLYILLVVCRGCIRSNSWSTRDAWGFNMHSYSIGYDIHGGEDGVSLGG 65
[Close]
BLAST of SMED30024478 vs. Planmine SMEST
Match: SMESG000000098.1 (SMESG000000098.1)

HSP 1 Score: 54.299 bits (129), Expect = 5.248e-9
Identity = 28/55 (50.91%), Postives = 36/55 (65.45%), Query Frame = 3 
Query: 1062 MDNATTFNK*GKARVQERASQHCNDDELTMERRTGAQ*GKINSYNSFEFNHYKFI 1226
            M+N    N+ GKARVQ+    H + DE+TME RTGAQ  KIN Y +  FN YK++
Sbjct:   14 MNNTGKSNRQGKARVQDYTPHHHSSDEITMEHRTGAQRSKINIYINSIFNSYKYL 68
The following BLAST results are available for this feature:
BLAST of SMED30024478 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024478 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024478 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024478 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024478 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024478 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024478 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024478 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024478 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024478 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024478 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024478 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024478 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024478 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000031417.15.553e-5696.51SMESG000031417.1[more]
SMESG000011009.18.660e-5392.13SMESG000011009.1[more]
SMESG000072325.11.066e-4590.00SMESG000072325.1[more]
SMESG000011009.11.169e-3490.77SMESG000011009.1[more]
SMESG000000098.15.248e-950.91SMESG000000098.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024478 ID=SMED30024478|Name=SMED30024478|organism=Schmidtea mediterranea sexual|type=transcript|length=1435bp
GACTAATGAGGAATTAAATTGTAAATCAAACGATAAAATCATAAAATACT
TTAGGGACTACCACCCAAATATAATAACGTAACGACTAGATTTAACAAAC
ACGTCATCACAAAATTTTTTTTAGATTAGGCAAAAATTTTTGAGAACGAA
ATTTAAACATTTACTTCTCAATAATCAAATCGAAAATCATACTAATTAAT
CAAGCTTTAATGATAGTAATCTAATGTAGCCTCCCATACATCAAATATAA
GAAAAGTCTTAACCAAACAATAATTAATGTAATCTTGATTAAATCTAACA
TAACCTTTAACCGAATAAAAGATAAAGAGCGATAAATTTAAAAATTGTAC
GCAGCAAATCCAAATTGTTGTCCCTTAAAATAGTCCAATAGCAAAACAAA
ATCCAAAATCACGTAATAAAAATAAGCATCTATTATACTAAGAATGCGCC
TAAGCTAACTCCATCTTCACCCCCATGAACATCATAACCGATTGAATAGG
AATGCATATTGAATCCCCAAGCGTCCCGAGTAGACCACGAATTGCTACGA
ATGCCGCCACGACAAACAACTAATAGGATATATAGAACCAAGTGACAATT
AATTGCGGGATGAGGAACAGTTGAATCATTACCCAACAGGGGAAACTCGA
AATCGACGAAGCAACGAATCCATGAAATGTAAATGAAATATTTTCCAATA
ACTAGACACATCAATTCATGGAAACACTATGGAAGAAGAATTGTAAAGAA
GAGCCGAAATGACGCGACTAACGAGTGACTCCAGTTATTCATTGGCCATT
AATTAACCATTTCCACGTGGCAGATATATGGAATATTGGATCGATTGCAA
GTAATAATTGGTGAACTTCCACGTTGCATATAAATGATCCGAAGAAATTT
GAATCGTTGAAAATTTTAAATCAATAAAATTCCACGCGACGTTAAGTTAA
TAAATCATAGATGTTATGCCATACACACATGCCCGATCACTAGTGTAAAC
CTGTGTGCGACTAAACTGATATAAATAAACAACCGAAGAAGAGGAAATTT
TATTTTTCACTATGGACAACGCGACCACATTCAACAAGTGAGGGAAAGCC
AGAGTACAAGAACGGGCCTCTCAACACTGCAATGATGACGAATTAACCAT
GGAGCGACGAACGGGCGCCCAATGAGGTAAAATTAACAGTTATAATAGTT
TTGAATTTAATCATTATAAATTTATATGAGATCAAACACCATGACGAGCA
TCCAGATTATTTGGCGATGAGAAATACAAATCCCAAAAAACGAAATGATT
ATAGATGAAAGAGGGAGTCAGCTCCGACGAGTGGAATGTGAATATTGATG
AATTAAATAAAAATTTAAATGCCGCGAATTTTCGATTTAAATCTTCCATG
CAAACATCGCCAATACACGAGATCGGAAGAGCGGT
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0003117parapharyngeal cell