Cell wall integrity and stress response component 1

Overview
NameCell wall integrity and stress response component 1
Smed IDSMED30024477
Length (bp)679
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Cell wall integrity and stress response component 1 (SMED30024477) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Cell wall integrity and stress response component 1 (SMED30024477) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30024477

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30024477h1SMcG0020073 SmedASXL_008793SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Match: A0A5P8I4L8 (Uncharacterized protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 199.904 bits (507), Expect = 1.510e-60
Identity = 113/230 (49.13%), Postives = 153/230 (66.52%), Query Frame = 3
Query:    3 RTGTNSLKIALEKLYNGKCYNIKEVFDYHQHHFEVWNSVLETEELRKDVAESILSEYCAVTDIPCSIVYKDLMEIYPDAKVILTIRNSDDWIKSVRMTTMTSEVHKMMRWKFLFWIFGSWTFMELVAKSISRIYKQPFELLMTNDVVLKKAYEEYNNEVRTFVPPERLLEFNVNEGWEPLCKXXXXXXXXXXXXXVNSTEEFLNYFNKKIKKYFAR--------IGCFSI 668
            RTGT SLK ALEKLYN  CY++ E+ ++H+ H ++W SV ++ ++ +  A  I S+Y A  D P + VYKDLMEIYP+AKV+LT RNSDDW+KSV+ TT+ + +      K LF+      F  ++ K   + YK+PF+ L T+D +LK+ YEE+N EVR +VP ERLLEFN  EGWEPLC+FLNLP PNEP+PFVN+T++F       + K   R        IGC S+
Sbjct:   17 RTGTFSLKFALEKLYNRPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQCYEEFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLPVPNEPYPFVNTTKDFQRNVRGMMLKSVIRSGLVLSLTIGCLSL 246          
BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Match: A0A183SJN6 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS4434 PE=4 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 5.384e-36
Identity = 85/212 (40.09%), Postives = 123/212 (58.02%), Query Frame = 3
Query:    3 RTGTNSLKIALEKLYNGKCYNIKEVFDYHQHHFEVWNSVLETEE---LRKDVA--ESILSEYCAVTDIPCSIVYKDLMEIYPDAKVILTIRNSDDWIKSVRMTTMTSEVHKM--MRWKFLFWIFGSWTFMELVAKSISRIYKQPFELLMTNDVVLKKAYEEYNNEVRTFVPPERLLEFNVNEGWEPLCKXXXXXXXXXXXXXVNSTEEFLNY 617
            RTGT SL+ ALEKLY   CY+  +   +     + W   L+  E   LR D A    +L  YC  TD P S+ +K+LM+ YP+AKVILT R+ D W+KS+R T    + +K   +   F+ W+ G    +++V + +    ++  + LM NDV L++A+ E+   V+  VP ERLL FN  EGW PLC FL+ P P+ PFP VN+++E   Y
Sbjct:   25 RTGTRSLQTALEKLYGEACYHTFDSMQHTPQDLQFWVESLDDMEGGVLRTDAAYWNRLLQGYCGTTDFPGSVFFKELMQAYPNAKVILTTRDPDAWVKSMRATVFPLDFYKSHGVFLNFVLWLKGITGILQIVDQGLRLSLRRSQDDLM-NDVQLRQAFLEWTAHVKKTVPSERLLIFNAQEGWGPLCHFLHRPIPDCPFPHVNASKEMKRY 235          
BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Match: A0A267F2Y8 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig019790g1 PE=4 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 1.243e-33
Identity = 67/182 (36.81%), Postives = 105/182 (57.69%), Query Frame = 3
Query:    3 RTGTNSLKIALEKLYNGKCYNIKEVFDYHQHHFEVWNSVLETEELRKDVAESILSEYCAVTDIPCSIVYKDLMEIYPDAKVILTIRNSDDWIKSVRMTTMTSEVHKMMRWKFLFWIFGSWTFMELVAKSISRIYKQPFELLMTNDVVLKKAYEEYNNEVRTFVPPERLLEFNVNEGWEPLCK 548
            RTGTNS   ALE L  G+CY++  V +    H ++W+     + L K++AE I  ++ A TD P +  YK+LM+IYPDAK +L+IR+  DW++SVR T + S++        + W+ G   ++ L    +       F++   +D  +  A+  +N+ V   +P ERLL F V++GWEPLCK
Sbjct:   15 RTGTNSQMKALEMLLGGRCYHMDIVMEEKLSHLQLWHRA-AIQGLSKELAEEIFDDFLACTDFPAAHFYKELMQIYPDAKFVLSIRDPTDWVRSVRATILQSKLTVEYMLPPVRWLLGLSHYLPLSDAVLGSRLGFNFDM---SDAEMIDAFNRHNDTVIRTIPKERLLVFRVSDGWEPLCK 192          
BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Match: A0A267EHD0 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig007040g1 PE=4 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 3.306e-33
Identity = 80/202 (39.60%), Postives = 118/202 (58.42%), Query Frame = 3
Query:    3 RTGTNSLKIALEKLYNGKCYNIKEVFDYHQHHFEVWNSVLETEELRKDVAESILSEYCAVTDIPCSIVYKDLMEIYPDAKVILTIRNSDDWIKSVRMTTMTSEVHKMMRWKFLFWIFGSWTFMELVAKSISRIYKQPFELLMTNDVVLKKAYEEYNNEVRTFVPPERLLEFNVNEGWEPLCKXXXXXXXXXXXXXVNSTEEF 608
            RTGTNS   ALE L  G+CY+++ + +  +HH ++W     TE L K++A+ +LSE+ A TD P +  Y +LM+IYP+AK IL++R  + W++SVR T   ++         + W+ G     +L      R+   P      +D  L  A++ +   V   +P ERLL F V +GWEPLCKFLN+P P+ PFP VN T+EF
Sbjct:    7 RTGTNSQMKALEILLGGRCYHMEVIIEEKRHHLKLWLKA-ATEGLTKELADEMLSEFRACTDFPAAHFYSELMQIYPEAKFILSLREPERWVQSVRATVYKAQSAGKHILPPIAWLIGFNYMDKLQTAMFPRMGMNP----GVSDQELAVAFDRHTEAVIRTIPKERLLVFRVTDGWEPLCKFLNVPVPDVPFPCVNDTKEF 203          
BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Match: A0A5J4NJ02 (Uncharacterized protein OS=Paragonimus westermani OX=34504 GN=DEA37_0011863 PE=4 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 3.382e-33
Identity = 78/212 (36.79%), Postives = 121/212 (57.08%), Query Frame = 3
Query:    3 RTGTNSLKIALEKLYNGKCYNIKEVFDYHQHHFEVW--------NSVLETEELRKDVAESILSEYCAVTDIPCSIVYKDLMEIYPDAKVILTIRNSDDWIKSVRMTTMTSEVHKMMRWKFLFW--IFGSWTFMELVAKSISRIYKQPFELLMTNDVVLKKAYEEYNNEVRTFVPPERLLEFNVNEGWEPLCKXXXXXXXXXXXXXVNSTEEF 608
            RTGTNSLK+ALE LYN  CY++K+++   +H    W        NS  +T +L K + + IL +Y +  D P    YK+L+++YP+AKVILT+RN + W+   R T +  ++     W F F   +FG     E+   S  R++ Q  +   +ND  +   +  +   V   VP +RLL +++++GWEPLC+FL+ P P+ PFP VN  +E 
Sbjct:   19 RTGTNSLKLALEILYNKPCYHLKDLYRNRRHGVARWLELEKRLKNS--KTGQLDKSLCDEILGKYGSAVDHPVCAYYKELLDVYPNAKVILTVRNPEIWLAGCRSTILPRDLFANRPWSFYFLRHVFGLKPLHEVYLDSWRRVFGQTMDF--SNDTAMMNGFVAWTERVTQTVPADRLLIYDISKGWEPLCRFLDKPVPDRPFPHVNEYQEL 226          
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Match: EDO49231 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGU4])

HSP 1 Score: 78.9518 bits (193), Expect = 7.599e-18
Identity = 55/183 (30.05%), Postives = 94/183 (51.37%), Query Frame = 3
Query:    3 RTGTNSLKIALEKLYNGKCYNIKEVFDYHQHHFEVWNSVLETEELRKDVAESILSEYCAVTDIPCSIVYKDLMEIYPDAKVILTIRNSDDWIKSVRMTTMTSEVHKMMRWKFLFWIFGSWTFMELVAKSISRIYKQPFELLMTNDV--VLKKAYEEYNNEVRTFVPPERLLEFNVNEGWEPLC 545
            +T T S+  AL  L     YN+ +V +     F+ W  ++E   L     E++     A+TD+P +  ++++++  PDAKVILT+R  + W++S +         KM++  F    F S + + L  + I+          +  +   ++ KA E +N  VR+ VPP +LLE++V EGW PLC
Sbjct:   10 KTATKSMAKALRIL----GYNVYDVEEQICELFDDWLDLIEHGRLPD--FEAMFENVDAITDLPGNFFFEEILKASPDAKVILTVREQEAWLRSSKKQL------KMVQGLFSKLWFLSLSPIYLGIRKITNGMVAALYGTINAESTWIIAKALEMHNQRVRSTVPPGQLLEYSVTEGWGPLC 180          
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Match: EDO41210 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4T7])

HSP 1 Score: 77.0258 bits (188), Expect = 5.195e-17
Identity = 59/185 (31.89%), Postives = 96/185 (51.89%), Query Frame = 3
Query:    3 RTGTNSLKIALEKLYNGKCYNIKEVFDYHQHHFEVWNSVLETEELRKDVAESILSEYCAVTDIPCSIVYKDLMEIYPDAKVILTIR-NSDDWIKSVRMTTMTSEVHKMMRWKFLFWIFGS-WTFMELVAKSISRIYKQPFELLMTNDVVL-KKAYEEYNNEVRTFVPPERLLEFNVNEGWEPLCK 548
            +TGT SL  AL ++   K Y+  E   YH    E W    ET ++  D+   +  +  AVTD+P    Y++L   +P+AKVIL++R N+D W +S+       +    +R    F +  + W     +++ +S +    F+    N  +L KKAY E+N  V+  +P ++LL F V +GW PLC+
Sbjct:   32 KTGTKSLAKAL-RILGLKVYDADEHCIYH---MEEWIQAFETSKM-PDLYR-MYKDVDAVTDVPPMFWYEELHYAFPEAKVILSVRDNTDVWYESLLWNQKVLKEAMSLRVMLGFLLTPTGWK----MSRMMSDMMNAAFKSTNPNAAILNKKAYREHNERVKNVIPKDKLLVFKVEQGWPPLCR 206          
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Match: EDO33663 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SRI4])

HSP 1 Score: 74.7146 bits (182), Expect = 1.181e-16
Identity = 56/184 (30.43%), Postives = 94/184 (51.09%), Query Frame = 3
Query:    3 RTGTNSLKIALEKLYNGKCYNIKEVFDYHQHHFEVWNSVLETEELRKDVAESILSEYCAVTDIPCSIVYKDLMEIYPDAKVILTIRNSDDWIKSVRMTTMTSEVHKMMRW-KFLFWIFGSWTFMELVAKSISRIYKQPF--ELLMTNDVVLKKAYEEYNNEVRTFVPPERLLEFNVNEGWEPLC 545
            +TGT S+  AL  L     Y + ++ +      + W  ++   +L     +S+  +  A+TD P S  +++++  +P AKV+LT+R+ D W +S+ +    +E  KM  W K     F S TF +L  K IS I +          +  + +K    +NN VR  VP ++LL F+V +GW+PLC
Sbjct:   10 KTGTKSMAKALRML----GYTVYDIAEQLGEFADEWTDLIIEGKLPD--FKSMFHDIDAITDYPGSFFFEEILAAFPQAKVVLTVRDEDAWARSLSLE--IAETAKM--WSKNSVLAFLSPTFAKL--KRISLIMETAMCGNFTPKSTFIHRKFIHMHNNRVRINVPKDQLLVFSVTQGWQPLC 181          
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Match: EDO48574 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RIV4])

HSP 1 Score: 69.707 bits (169), Expect = 4.292e-14
Identity = 55/182 (30.22%), Postives = 91/182 (50.00%), Query Frame = 3
Query:    3 RTGTNSLKIALEKLYNGKCYNIKEVFDYHQHHFEVWNSVLETEELRKDVAESILSEYCAVTDIPCSIVYKDLMEIYPDAKVILTIRNSDD-WIKSVRMTTMTSEVHKMMRWKFLFWIFGSWTFMELVAKSISRIYKQPFELLMTNDVVLKKAYEEYNNEVRTFVPPERLLEFNVNEGWEPLC 545
            +TGT SL  AL  L     YN+ +  +  Q H + + +  E +       E + +   A TD+P SI YK++ + +PDAKVIL++R+ ++ W +S+  T     V   M    L  +  + T      +  SRI +      + N +  +  Y ++N +V   +P ++LL FN  +GWEPLC
Sbjct:  165 KTGTKSLASALRHL----GYNVHDHPEQIQFHCDEYIAAFEGQMPN---FEEMYANVDATTDLPASIFYKEIFKAFPDAKVILSVRDDEESWRRSLLKTV---GVVSSMTNNPLLIVCRTLT---PTGRKYSRILR-----FIMNYLKNEIDYTKHNGDVMANIPAKQLLVFNPKDGWEPLC 328          
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Match: EDO39565 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S9S8])

HSP 1 Score: 65.855 bits (159), Expect = 3.386e-13
Identity = 50/188 (26.60%), Postives = 92/188 (48.94%), Query Frame = 3
Query:    3 RTGTNSLKIALEKLYNGKCYNIKEVFDYHQHHFEVWNSVLET-----EELRKDVAESILSEYCAVTDIPCSIVYKDLMEIYPDAKVILTIRNSDD-WIKS-VRMTTMTSEVHKMMRWKFLFWIFGSWTFMELVAKSISRIYKQPFELLMTNDVVLKKAYEEYNNEVRTFVPPERLLEFNVNEGWEPLC 545
            +TGT SL  AL +L     +N+ +  +  Q H + + +  +      EE+ ++V         A TD+P  I YK++ + +PDAKVIL++R+ ++ W KS ++  ++ S     +      ++  S   +  +  ++ +  K   +            Y ++NNEV   +P ++LL FN  +GWE LC
Sbjct:   10 KTGTKSLASALRRL----GFNVHDHHEQLQFHCDEYIAAFKGPMPNFEEMYENVD--------ATTDLPACIFYKEIFKAFPDAKVILSVRDDEESWRKSFLKTESIVSSATSNLLMILGLFLTPSGRKLWRLGYNVKKYVKNELD------------YTKHNNEVMATIPAKQLLVFNPKDGWESLC 173          
BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Match: SMESG000035035.1 (SMESG000035035.1)

HSP 1 Score: 433.335 bits (1113), Expect = 2.744e-156
Identity = 225/225 (100.00%), Postives = 225/225 (100.00%), Query Frame = 3
Query:    3 RTGTNSLKIALEKLYNGKCYNIKEVFDYHQHHFEVWNSVLETEELRKDVAESILSEYCAVTDIPCSIVYKDLMEIYPDAKVILTIRNSDDWIKSVRMTTMTSEVHKMMRWKFLFWIFGSWTFMELVAKSISRIYKQPFELLMTNDVVLKKAYEEYNNEVRTFVPPERLLEFNVNEGWEPLCKXXXXXXXXXXXXXVNSTEEFLNYFNKKIKKYFARIGCFSIFFG 677
            RTGTNSLKIALEKLYNGKCYNIKEVFDYHQHHFEVWNSVLETEELRKDVAESILSEYCAVTDIPCSIVYKDLMEIYPDAKVILTIRNSDDWIKSVRMTTMTSEVHKMMRWKFLFWIFGSWTFMELVAKSISRIYKQPFELLMTNDVVLKKAYEEYNNEVRTFVPPERLLEFNVNEGWEPLCKFLNLPFPNEPFPFVNSTEEFLNYFNKKIKKYFARIGCFSIFFG
Sbjct:   10 RTGTNSLKIALEKLYNGKCYNIKEVFDYHQHHFEVWNSVLETEELRKDVAESILSEYCAVTDIPCSIVYKDLMEIYPDAKVILTIRNSDDWIKSVRMTTMTSEVHKMMRWKFLFWIFGSWTFMELVAKSISRIYKQPFELLMTNDVVLKKAYEEYNNEVRTFVPPERLLEFNVNEGWEPLCKFLNLPFPNEPFPFVNSTEEFLNYFNKKIKKYFARIGCFSIFFG 234          
BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Match: SMESG000035040.1 (SMESG000035040.1)

HSP 1 Score: 222.631 bits (566), Expect = 4.725e-73
Identity = 124/226 (54.87%), Postives = 154/226 (68.14%), Query Frame = 3
Query:    3 RTGTNSLKIALEKLYNGKCYNIKEVFDYHQHHFEVWNSVLETEELRKDVAESILSEYCAVTDIPCSIVYKDLMEIYPDAKVILTIRNSDDWIKSVRMTTMTSEVHKMMRWKFLFWIFGSWTFMELVAKSISRIYKQPFELLMTNDVVLKKAYEEYNNEVRTFVPPERLLEFNVNEGWEPLCKXXXXXXXXXXXXXVNSTEEFLNYFNKKIKKYFARIGCF-SIFFG 677
            RTGT SLK+ALEKLYNGKCY++ E+F+ H++H  +W SV +   L K  AES+LS+YCA  D P + VYKDLM++YP+AKVILT RNSDDW+KSVR TT+          K  F   G   F E   K I+  YK  +E L+ +D +LK+ YE++N EVR  VP ERLLEFN  +GWEPLC FLNLP P+EP+PFVNSTEEF  Y NK     F + G F +IF G
Sbjct:   17 RTGTKSLKMALEKLYNGKCYHMAEIFENHRNHISLWISVFDKNGLDKSTAESLLSDYCATVDFPSTAVYKDLMKLYPNAKVILTTRNSDDWLKSVRNTTLKKGSDVTFLSKIFFMFSGLGRFPEFSEKLINHAYKNSYETLINDDAILKQCYEDFNQEVRETVPKERLLEFNSKQGWEPLCTFLNLPIPDEPYPFVNSTEEFNKYINKTYLADFVKYGIFVTIFAG 242          
BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Match: SMESG000035096.1 (SMESG000035096.1)

HSP 1 Score: 199.904 bits (507), Expect = 5.052e-64
Identity = 112/230 (48.70%), Postives = 152/230 (66.09%), Query Frame = 3
Query:    3 RTGTNSLKIALEKLYNGKCYNIKEVFDYHQHHFEVWNSVLETEELRKDVAESILSEYCAVTDIPCSIVYKDLMEIYPDAKVILTIRNSDDWIKSVRMTTMTSEVHKMMRWKFLFWIFGSWTFMELVAKSISRIYKQPFELLMTNDVVLKKAYEEYNNEVRTFVPPERLLEFNVNEGWEPLCKXXXXXXXXXXXXXVNSTEEFLNYFNKKIKKYFAR--------IGCFSI 668
            RTGT SLK ALEKLYN  CY++ E+ ++H+ H ++W SV ++ ++ +  A  I S+Y A  D P + VYKDLMEIYP+AKV+LT RNSDDW+KSVR TT+  E+   +  KFL +      F  +  K   + YK+PF+ +  +D +LK+ YE++N EVR +VP ERLLEFN  EGWEPLC+FLNLP PNEP+PFVN+T++F       + K   R        IGC S+
Sbjct:   17 RTGTFSLKFALEKLYNRPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVRSTTLKDEMRTSLFDKFLLFFSIGTEFPVMCDKMFLKAYKKPFDAVRADDELLKQCYEDFNEEVRIYVPKERLLEFNAKEGWEPLCRFLNLPVPNEPYPFVNTTQDFQRNVRGIMLKSLIRSGLAISLTIGCLSL 246          
BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Match: SMESG000035037.1 (SMESG000035037.1)

HSP 1 Score: 199.134 bits (505), Expect = 7.552e-64
Identity = 111/230 (48.26%), Postives = 152/230 (66.09%), Query Frame = 3
Query:    3 RTGTNSLKIALEKLYNGKCYNIKEVFDYHQHHFEVWNSVLETEELRKDVAESILSEYCAVTDIPCSIVYKDLMEIYPDAKVILTIRNSDDWIKSVRMTTMTSEVHKMMRWKFLFWIFGSWTFMELVAKSISRIYKQPFELLMTNDVVLKKAYEEYNNEVRTFVPPERLLEFNVNEGWEPLCKXXXXXXXXXXXXXVNSTEEFLNYFNKKIKKYFAR--------IGCFSI 668
            RTGT SLK ALEKLYN  CY++ E+ ++H+ H ++W SV ++ ++ +  A  I S+Y A  D P + VYKDLMEIYP+AKV+LT RNSDDW+KSV+ TT+ + +      K LF+      F  ++ K   + YK+PF+ L T+D +LK+ YE++N EVR +VP ERLLEFN  EGWEPLC+FLNL  PNEP+PFVN+T++F       + K   R        IGC S+
Sbjct:   17 RTGTFSLKFALEKLYNCPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKMFLKAYKEPFDALRTDDELLKQCYEDFNEEVRKYVPKERLLEFNAKEGWEPLCRFLNLQVPNEPYPFVNTTKDFQRNVRGMMLKSVIRSGLVLSLTIGCLSL 246          
BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Match: SMESG000035036.1 (SMESG000035036.1)

HSP 1 Score: 198.364 bits (503), Expect = 1.802e-63
Identity = 113/230 (49.13%), Postives = 153/230 (66.52%), Query Frame = 3
Query:    3 RTGTNSLKIALEKLYNGKCYNIKEVFDYHQHHFEVWNSVLETEELRKDVAESILSEYCAVTDIPCSIVYKDLMEIYPDAKVILTIRNSDDWIKSVRMTTMTSEVHKMMRWKFLFWIFGSWTFMELVAKSISRIYKQPFELLMTNDVVLKKAYEEYNNEVRTFVPPERLLEFNVNEGWEPLCKXXXXXXXXXXXXXVNSTEEFLNYFNKKIKKYFAR--------IGCFSI 668
            RTGT SLK ALEKLYN  CY++ E+ ++H+ H ++W SV ++ ++ +  A  I S+Y A  D P + VYKDLMEIYP+AKV+LT RNSDDW+KSV+ TT+ + +      K LF+      F  ++ K   + YK+PF+ L T+D +LK+ YE++N EVR +VP ERLLEFN  EGWEPLC+FLNLP PNEP+PFVN+T++F       + K   R        IGC SI
Sbjct:   17 RTGTFSLKFALEKLYNCPCYHMAEILNHHKDHVKLWISVFDSGKMNEKTAGKIFSDYSATVDFPSAAVYKDLMEIYPNAKVVLTTRNSDDWVKSVKSTTLRNNMKTSFVHKILFYFNLGTEFPVMLDKMYLKAYKEPFDALRTDDELLKQCYEDFNEEVRKYVPRERLLEFNAKEGWEPLCRFLNLPVPNEPYPFVNTTKDFQLTIRGMMLKSVIRSGLVLSLTIGCLSI 246          
The following BLAST results are available for this feature:
BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5P8I4L81.510e-6049.13Uncharacterized protein OS=Schmidtea mediterranea ... [more]
A0A183SJN65.384e-3640.09Uncharacterized protein OS=Schistocephalus solidus... [more]
A0A267F2Y81.243e-3336.81Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A267EHD03.306e-3339.60Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A5J4NJ023.382e-3336.79Uncharacterized protein OS=Paragonimus westermani ... [more]
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO492317.599e-1830.05Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO412105.195e-1731.89Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO336631.181e-1630.43Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO485744.292e-1430.22Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO395653.386e-1326.60Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Cell wall integrity and stress response component 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Cell wall integrity and stress response component 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000035035.12.744e-156100.00SMESG000035035.1[more]
SMESG000035040.14.725e-7354.87SMESG000035040.1[more]
SMESG000035096.15.052e-6448.70SMESG000035096.1[more]
SMESG000035037.17.552e-6448.26SMESG000035037.1[more]
SMESG000035036.11.802e-6349.13SMESG000035036.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024477 ID=SMED30024477|Name=Cell wall integrity and stress response component 1|organism=Schmidtea mediterranea sexual|type=transcript|length=679bp
TGAGAACCGGCACAAATTCGTTAAAAATCGCACTGGAAAAATTATACAAT
GGCAAATGTTATAATATTAAAGAGGTGTTTGATTATCATCAACATCATTT
TGAAGTATGGAACTCAGTTCTAGAGACGGAGGAACTGAGAAAAGATGTCG
CAGAAAGTATTTTATCAGAATACTGTGCAGTAACTGATATTCCGTGTTCA
ATAGTTTACAAAGATTTGATGGAAATATACCCAGATGCTAAGGTTATATT
GACGATTCGAAACTCGGATGATTGGATAAAGAGCGTTAGAATGACAACAA
TGACGAGCGAAGTTCATAAAATGATGCGATGGAAATTTCTATTCTGGATA
TTTGGTTCTTGGACATTCATGGAGCTTGTTGCGAAATCAATTAGTAGAAT
TTACAAGCAACCCTTTGAACTTTTAATGACAAACGATGTTGTATTAAAAA
AGGCTTACGAAGAGTATAATAATGAGGTTAGGACTTTTGTTCCTCCAGAA
AGACTTCTTGAATTTAATGTCAATGAAGGATGGGAACCGTTGTGTAAATT
TTTGAATTTACCTTTTCCCAATGAACCTTTTCCATTTGTCAATTCGACGG
AAGAGTTTTTAAATTATTTTAATAAAAAAATTAAAAAGTATTTTGCTAGA
ATTGGATGCTTTTCCATATTTTTTGGATT
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protein sequence of SMED30024477-orf-1

>SMED30024477-orf-1 ID=SMED30024477-orf-1|Name=SMED30024477-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=225bp
RTGTNSLKIALEKLYNGKCYNIKEVFDYHQHHFEVWNSVLETEELRKDVA
ESILSEYCAVTDIPCSIVYKDLMEIYPDAKVILTIRNSDDWIKSVRMTTM
TSEVHKMMRWKFLFWIFGSWTFMELVAKSISRIYKQPFELLMTNDVVLKK
AYEEYNNEVRTFVPPERLLEFNVNEGWEPLCKFLNLPFPNEPFPFVNSTE
EFLNYFNKKIKKYFARIGCFSIFFG
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR040632Sulfotransfer_4
IPR027417P-loop_NTPase
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR040632Sulfotransferase, S. mansonii-typePFAMPF17784Sulfotransfer_4coord: 1..202
e-value: 2.3E-56
score: 191.0
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 1..218
e-value: 5.5E-61
score: 208.4
NoneNo IPR availablePANTHERPTHR36978FAMILY NOT NAMEDcoord: 1..214
NoneNo IPR availableTMHMMTMhelixcoord: 112..134
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 1..217