SMED30024468

Overview
NameSMED30024468
Smed IDSMED30024468
Length (bp)1696
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30024468 (SMED30024468) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30024468 (SMED30024468) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30024468 (SMED30024468) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30024468 (SMED30024468) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30024468

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30024468h1SMcG0000711 SmedASXL_016176SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30024468h1SMcG0000711 SmedASXL_016176SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X1 cellSMED30024468h1SMcG0000711 SmedASXL_016175SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30024468h1SMcG0000711 SmedASXL_016175SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30024468h1SMcG0000711 dd_Smed_v4_12953_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30024468h1SMcG0000711 dd_Smed_v6_12953_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
zeta neoblastSMED30024468h1SMcG0000711 dd_Smed_v4_12953_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30024468 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.004365:4013..12175 +8159
v31.018423:5952..14025 +8135
Homology
[Close]
BLAST of SMED30024468 vs. Ensembl Zebrafish
Match: dlgap1b (discs, large (Drosophila) homolog-associated protein 1b [Source:ZFIN;Acc:ZDB-GENE-091116-98])

HSP 1 Score: 50.0618 bits (118), Expect = 5.936e-6
Identity = 32/91 (35.16%), Postives = 51/91 (56.04%), Query Frame = 2 
Query:  659 ERETDIPLEYQGDIKSAIGGLKMLLENRFQSTLDLLDAAEAYVNNNGKPVNDTNQHYLSGDIIGFTXXXXXXXXXXXXXFNKLYNLRKNNW 931
            ERE ++P +  G I+SA+G  ++L+  +FQ   DL    E  +N N  P        +S D+ GF D+L   I+ I + F++L+ L+ NNW
Sbjct:  503 ERENELPEDILGKIRSAVGSAQLLMAQKFQQFRDL---CEENLNPNAHP------RPMSQDLAGFWDMLQLSIENISLKFDELHQLKANNW 584
[Close]
BLAST of SMED30024468 vs. TrEMBL
Match: G4VDE7 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 GN=Smp_143460 PE=4 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 3.707e-7
Identity = 91/368 (24.73%), Postives = 158/368 (42.93%), Query Frame = 2 
Query:   80 MNKEIHISQVPLTPEGVNHDFNAVK------DYEFVRYSEIMKVRTKSMIWSEDYISDNE-----------VEFSTLETDEQIIDALVNSPMIEVRKKRKSINCS-----LDGSIRFNPLR----DDILETSAGSYHNYQQNRRKTI-----------AVMKRLSSPLKRQPRKL--RQSITPLTTKENLDPT-------LKVEKEIELLV--------AAVDHSQTLLNIEKIRLSDFIKDWSHILERETD-IPLEYQGDIKSAIGGLKMLLENRFQSTLDLLDAAEAYVNNNGKPVND-TNQHYLSGDIIGFTXXXXXXXXXXXXXFNKLYNLRKN-NWVEDSVK-TPETIKI------PVKKIKK 991
            M K +HI  VP TPEG + DF  +       D E    S +++      IW E+  SD++           ++   + +++    A+ +SPM++ R +R S + S     LD S    PLR    DD++ +++        NRR ++           +   + ++P+ +  R+   ++S +    KEN+ P+         V KEI   V         +V   + L ++E  RL     DW+ +L  E D IP +    I++ +G  ++LL+ +      L++ AE  +  +    N  T       D++G+  L+ DEI   D  F +L   R   +W  D    TP   +I      P K+IK+
Sbjct:    1 MRKRVHIDVVPKTPEGGHIDFRQISADEIEFDSEITIKSSLLRECKPGNIWFEEGDSDDDDNKPKFVARKSMDLRKIRSEKDRFQAICSSPMVDSRPRRLSYSKSFSEGVLDPSNSKTPLRLPKYDDLVSSASRRLSRIIINRRFSLLETNHNDPSAGSSQLKTTTPITKYERQRVKKKSSSTSEEKENIKPSNNDIITATSVSKEIFDDVPNSSVHDPCSVSEFRVLADMETARLLALCGDWNCVLSEECDNIPEKALDSIRATVGKCQLLLQKKLPFFKTLIEMAETSLGKSDTLSNSATAPEATINDLLGYWTLVADEISLSDSAFERLRVWRDELHWSIDQCPVTPARSQIVKRRGRPSKQIKR 368
[Close]
BLAST of SMED30024468 vs. TrEMBL
Match: A0A430QJT8 (Uncharacterized protein OS=Schistosoma bovis OX=6184 GN=DC041_0002158 PE=4 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.080e-6
Identity = 72/314 (22.93%), Postives = 135/314 (42.99%), Query Frame = 2 
Query:   80 MNKEIHISQVPLTPEGVNHDFNAVK------DYEFVRYSEIMKVRTKSMIWSEDYISDNE-----------VEFSTLETDEQIIDALVNSPMIEVRKKRKSINCS-----LDGSIRFNPLR----DDILETSAGSYHNYQQNRRKTI------------AVMKRLSSPLKRQPRKLRQSITPLT-TKENLDPT-------LKVEKEIELLV--------AAVDHSQTLLNIEKIRLSDFIKDWSHILERETD-IPLEYQGDIKSAIGGLKMLLENRFQSTLDLLDAAEAYVNNNGKPVND-TNQHYLSGDIIGF 853
            M K +HI  VP TPEG + DF  +       D E    S +++      +W E+  SD+E           ++   + +++    A+ +SPM++ R +R S + S     LD S    PLR    DD++ +++        NRR ++            + +K  +   K + +++++  +  +  KEN+ P+         V KE+   V         +V   + L ++E  RL     DW+ +L  E D IP +    I++ +G  ++LL+ +      L++ AE  +  +    N  T       D++G+
Sbjct:    1 MRKRVHIDVVPKTPEGGHIDFRQISAEEIEFDSEITIKSSLLRECKPGNVWFEEGDSDDEDKKPKFVARKSMDLRKIRSEKDRFQAICSSPMVDSRPRRLSYSKSFSEGVLDPSNSKTPLRLPKYDDLVSSASRRLSRIIINRRFSLLETNHNDPSVEGSQLKTTTPITKYEKQRVKKKCSSASEEKENIKPSNNDIVTATPVSKEVFDDVSNSSAHDPCSVSEFRVLADMETARLLALCGDWNCVLSEECDNIPEKALDSIRATVGKCQLLLQKKLPFFKSLIEMAETSLGKSDTLSNSATAPEATINDLLGY 314
[Close]
BLAST of SMED30024468 vs. TrEMBL
Match: A0A183P9R2 (Uncharacterized protein OS=Schistosoma mattheei OX=31246 GN=SMTD_LOCUS11098 PE=4 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 6.618e-6
Identity = 71/314 (22.61%), Postives = 134/314 (42.68%), Query Frame = 2 
Query:   80 MNKEIHISQVPLTPEGVNHDFNAVK------DYEFVRYSEIMKVRTKSMIWSEDYISDNE-----------VEFSTLETDEQIIDALVNSPMIEVRKKRKSINCS-----LDGSIRFNPL----RDDILETSAGSYHNYQQNRRKTI------------AVMKRLSSPLKRQPRKLRQSITPLT-TKENLDP-------TLKVEKEIELLV--------AAVDHSQTLLNIEKIRLSDFIKDWSHILERETD-IPLEYQGDIKSAIGGLKMLLENRFQSTLDLLDAAEAYVNNNGKPVND-TNQHYLSGDIIGF 853
            M K +HI  VP TPEG + DF  +       D E    S +++      +W E+  SD++           ++   + +++    A+ +SPM++ R +R S + S     LD S    PL     DD++ +++        NRR ++            + +K  +   K + +++++  +  +  KEN+ P       T  V KE+   V         +V   + L ++E  RL     DW+ +L  E D IP +    I++ +G  ++LL+ +      L++ AE  +  +    N  T       D++G+
Sbjct:    1 MRKRVHIDVVPKTPEGGHIDFRQISAEEIEFDSEITIKSSLLRECKPGNVWFEEGDSDDDDNKPKFVARKSMDLRKIRSEKDRFQAICSSPMVDSRPRRLSYSKSFSEGVLDPSNSKTPLCLPKYDDLVSSASRRLSRIIINRRFSLLETNHNDPSVGGSQLKTTTPITKYEKQRVKKKCSSASEEKENIKPSNNDIVTTTPVSKEVFDDVSNSSAHDPCSVSEFRVLADMETARLLALCGDWNCVLSEECDNIPEKALDSIRATVGKCQLLLQKKLPFFKSLIEMAETSIGKSDTLSNSATAPEATINDLLGY 314
[Close]
BLAST of SMED30024468 vs. TrEMBL
Match: A0A3P7M1E8 (Uncharacterized protein OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS7972 PE=4 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 7.655e-6
Identity = 91/352 (25.85%), Postives = 143/352 (40.62%), Query Frame = 2 
Query:   80 MNKEIHISQVPLTPEGVNHDFNAVKDYEFVRYSEI--------------MKVRT----KSMIWS---EDYISDNEVEFSTLETDEQIIDALVNSPMIEVRKKRKSINCSLDGSIRFNPLRDDILETSAG-SYHNYQQNRRKTIAVMKRLSSPLKRQPRKLRQSIT----------PLTTKENLDPTLKVEKEIELLVA------AVDHSQTLLNIEKIRLSDFIKDWSHILERE-----------------TDIPLEYQGDIKSAIGGLKMLLENRFQSTLDLLDAAEAYVNN---NGKPVNDTNQHYLSGDIIGFTXXXXXXXXXXXXXFNKLYNLRKNN-W-VEDSVKTP 955
            M+K   IS VP TP GV+  F  V   E     EI              MK R+    KS++     E ++     +    +T E  ++AL++SPMIEVR++       L  S  F  +   IL+  A     +   NR   I  +  L S     PR L   +           P+   EN+ P  + E   E + A      ++   + L+++E  RL      W  ++E E                 T++ L     I+ A+G   +LL  +F    +L+D A+A  N+      P  D N      D++G+   +  EI++ID  F +L   R+N+ W +E   KTP
Sbjct:    1 MSKRTLISDVPQTPGGVHLCFGDVSADEIAVDEEISIKSSLFRTSTEPHMKTRSSSRKKSLVTPKTKEKFVVRKSTDLRRFKTVEDRVNALISSPMIEVRRR------PLSQSQSFTSMEYLILQWWAVIQCADAILNRNAKIVCVGWLVSRSLVAPRTLESPVQEAANLDAEPEPMDDSENIPPGTETEPAPETVQAEQMEPSSIGEFRVLVDMEAARLKAARAAWEDVVEEEGSAIPDVGNTKTIAVEITELYLSALDSIRVAVGKCGLLLAKKFPFFHNLIDMADASPNSATEESAPSADIN------DLLGYWTTIAAEIEQIDSAFERLRVWRENSKWALESRPKTP 340
[Close]
BLAST of SMED30024468 vs. Planmine SMEST
Match: SMESG000047438.1 (SMESG000047438.1)

HSP 1 Score: 996.882 bits (2576), Expect = 0.000e+0
Identity = 506/511 (99.02%), Postives = 507/511 (99.22%), Query Frame = 2 
Query:   80 MNKEIHISQVPLTPEGVNHDFNAVKDYEFVRYSEIMKVRTKSMIWSEDYISDNEVEFSTLETDEQIIDALVNSPMIEVRKKRKSINCSLDGSIRFNPLRDDILETSAGSYHNYQQNRRKTIAVMKRLSSPLKRQPRKLRQSITPLTTKENLDPTLKVEKEIELLVAAVDHSQTLLNIEKIRLSDFIKDWSHILERETDIPLEYQGDIKSAIGGLKMLLENRFQSTLDLLDAAEAYVNNNGKPVNDTNQHYLSGDIIGFTXXXXXXXXXXXXXFNKLYNLRKNNWVEDSVKTPETIKIPVKKIKKGLQKKTVQSKINMALLRMKYQVNKANSIKSPEMLIEETVEIDSNIPTKVRRSKRHIEKPAEADENMDETNENYNKDSEQRNETRHLSTLGNIIRPKSILKRKTGRSSISDKTDLRFDLEKKNDCSNENSSKDVINLSKTPTSLRKGIRSKSILKSDTGTPVHEDTPRPIKVRKEAKEFAIPSSSSFFPKNKQQKRASHVVTSEYMNS 1612
            MNKEIHISQVPLTPEGVNHDFNAVKDYEFVRYSEIMKVRTKSMIWSEDYISDNEVEFSTLETDEQIIDALVNSPMIEVRKKRKSINCSLDGSIRFNPLRDDILETSAGSYHNYQQNRRKTIAVMKRLSSPLKRQPRKLRQSITPLTTKENLDPTLKVEKEIELLVAAVDHSQTLLNIEKIRLSDFIKDWSHILERETDIPLEYQGDIKSAIGGLKMLLENRFQSTLDLLDAAEAYVNNNGKPVNDTNQHYLSGDIIGFTDLLIDEIKKIDIDFNKLYNLRKNNWVEDSVKTPETIKIPVKKIKKGLQKKTVQSKINMALLRMKYQVNKANSIKSP MLIEE VEIDSNIPTKVRRSKRHIEKPAEADENMDETNENYNKDSEQRNETRHLSTLGNIIRPKSILKRKTGRSSISDKTDLRFDLEKKNDCSNENSSKDVINLSKTPTSLRKGIRSKSILKSDTGTPVHEDTPRPIKVRKEAKEFAIPSSSSFFPKNKQQKRASHVVTSE + S
Sbjct:    1 MNKEIHISQVPLTPEGVNHDFNAVKDYEFVRYSEIMKVRTKSMIWSEDYISDNEVEFSTLETDEQIIDALVNSPMIEVRKKRKSINCSLDGSIRFNPLRDDILETSAGSYHNYQQNRRKTIAVMKRLSSPLKRQPRKLRQSITPLTTKENLDPTLKVEKEIELLVAAVDHSQTLLNIEKIRLSDFIKDWSHILERETDIPLEYQGDIKSAIGGLKMLLENRFQSTLDLLDAAEAYVNNNGKPVNDTNQHYLSGDIIGFTDLLIDEIKKIDIDFNKLYNLRKNNWVEDSVKTPETIKIPVKKIKKGLQKKTVQSKINMALLRMKYQVNKANSIKSPGMLIEEIVEIDSNIPTKVRRSKRHIEKPAEADENMDETNENYNKDSEQRNETRHLSTLGNIIRPKSILKRKTGRSSISDKTDLRFDLEKKNDCSNENSSKDVINLSKTPTSLRKGIRSKSILKSDTGTPVHEDTPRPIKVRKEAKEFAIPSSSSFFPKNKQQKRASHVVTSECVKS 511
[Close]
BLAST of SMED30024468 vs. Planmine SMEST
Match: SMESG000047438.1 (SMESG000047438.1)

HSP 1 Score: 862.062 bits (2226), Expect = 0.000e+0
Identity = 439/441 (99.55%), Postives = 439/441 (99.55%), Query Frame = 2 
Query:   80 MNKEIHISQVPLTPEGVNHDFNAVKDYEFVRYSEIMKVRTKSMIWSEDYISDNEVEFSTLETDEQIIDALVNSPMIEVRKKRKSINCSLDGSIRFNPLRDDILETSAGSYHNYQQNRRKTIAVMKRLSSPLKRQPRKLRQSITPLTTKENLDPTLKVEKEIELLVAAVDHSQTLLNIEKIRLSDFIKDWSHILERETDIPLEYQGDIKSAIGGLKMLLENRFQSTLDLLDAAEAYVNNNGKPVNDTNQHYLSGDIIGFTXXXXXXXXXXXXXFNKLYNLRKNNWVEDSVKTPETIKIPVKKIKKGLQKKTVQSKINMALLRMKYQVNKANSIKSPEMLIEETVEIDSNIPTKVRRSKRHIEKPAEADENMDETNENYNKDSEQRNETRHLSTLGNIIRPKSILKRKTGRSSISDKTDLRFDLEKKNDCSNENSSKDVINLS 1402
            MNKEIHISQVPLTPEGVNHDFNAVKDYEFVRYSEIMKVRTKSMIWSEDYISDNEVEFSTLETDEQIIDALVNSPMIEVRKKRKSINCSLDGSIRFNPLRDDILETSAGSYHNYQQNRRKTIAVMKRLSSPLKRQPRKLRQSITPLTTKENLDPTLKVEKEIELLVAAVDHSQTLLNIEKIRLSDFIKDWSHILERETDIPLEYQGDIKSAIGGLKMLLENRFQSTLDLLDAAEAYVNNNGKPVNDTNQHYLSGDIIGFTDLLIDEIKKIDIDFNKLYNLRKNNWVEDSVKTPETIKIPVKKIKKGLQKKTVQSKINMALLRMKYQVNKANSIKSP MLIEE VEIDSNIPTKVRRSKRHIEKPAEADENMDETNENYNKDSEQRNETRHLSTLGNIIRPKSILKRKTGRSSISDKTDLRFDLEKKNDCSNENSSKDVINLS
Sbjct:    1 MNKEIHISQVPLTPEGVNHDFNAVKDYEFVRYSEIMKVRTKSMIWSEDYISDNEVEFSTLETDEQIIDALVNSPMIEVRKKRKSINCSLDGSIRFNPLRDDILETSAGSYHNYQQNRRKTIAVMKRLSSPLKRQPRKLRQSITPLTTKENLDPTLKVEKEIELLVAAVDHSQTLLNIEKIRLSDFIKDWSHILERETDIPLEYQGDIKSAIGGLKMLLENRFQSTLDLLDAAEAYVNNNGKPVNDTNQHYLSGDIIGFTDLLIDEIKKIDIDFNKLYNLRKNNWVEDSVKTPETIKIPVKKIKKGLQKKTVQSKINMALLRMKYQVNKANSIKSPGMLIEEIVEIDSNIPTKVRRSKRHIEKPAEADENMDETNENYNKDSEQRNETRHLSTLGNIIRPKSILKRKTGRSSISDKTDLRFDLEKKNDCSNENSSKDVINLS 441
[Close]
BLAST of SMED30024468 vs. Planmine SMEST
Match: SMESG000028235.1 (SMESG000028235.1)

HSP 1 Score: 280.026 bits (715), Expect = 2.541e-92
Identity = 139/142 (97.89%), Postives = 140/142 (98.59%), Query Frame = 2 
Query: 1187 MDETNENYNKDSEQRNETRHLSTLGNIIRPKSILKRKTGRSSISDKTDLRFDLEKKNDCSNENSSKDVINLSKTPTSLRKGIRSKSILKSDTGTPVHEDTPRPIKVRKEAKEFAIPSSSSFFPKNKQQKRASHVVTSEYMNS 1612
            MDETNENYNKDSEQRNETRHLSTLGNIIRPKSILKRKTGRSSISDKTDLRFDLEKKNDCSNENSSKDVINLSKTPTSLRKGIRSKSILKSDTGTPVHEDTPRPIKVRKEAKEFAIPSSSSFFPKNKQQKRASHVVTSE + S
Sbjct:    1 MDETNENYNKDSEQRNETRHLSTLGNIIRPKSILKRKTGRSSISDKTDLRFDLEKKNDCSNENSSKDVINLSKTPTSLRKGIRSKSILKSDTGTPVHEDTPRPIKVRKEAKEFAIPSSSSFFPKNKQQKRASHVVTSECVKS 142
The following BLAST results are available for this feature:
BLAST of SMED30024468 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024468 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024468 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024468 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
dlgap1b5.936e-635.16discs, large (Drosophila) homolog-associated prote... [more]
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BLAST of SMED30024468 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024468 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024468 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024468 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 4
Match NameE-valueIdentityDescription
G4VDE73.707e-724.73Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A430QJT81.080e-622.93Uncharacterized protein OS=Schistosoma bovis OX=61... [more]
A0A183P9R26.618e-622.61Uncharacterized protein OS=Schistosoma mattheei OX... [more]
A0A3P7M1E87.655e-625.85Uncharacterized protein OS=Dibothriocephalus latus... [more]
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BLAST of SMED30024468 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024468 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024468 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024468 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024468 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024468 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000047438.10.000e+099.02SMESG000047438.1[more]
SMESG000047438.10.000e+099.55SMESG000047438.1[more]
SMESG000028235.12.541e-9297.89SMESG000028235.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024468 ID=SMED30024468|Name=SMED30024468|organism=Schmidtea mediterranea sexual|type=transcript|length=1696bp
GTTTAAATAAACATATGAATTAAAACTTTGATAAAGAATTTAATTTAATA
GTTTAAAAGTTTTGATTAACTGATTTGCGATGAATAAAGAAATCCATATT
TCCCAAGTTCCACTAACTCCCGAAGGAGTTAATCATGATTTCAATGCCGT
TAAAGATTACGAATTTGTAAGATACAGTGAAATAATGAAAGTGAGAACAA
AATCTATGATATGGTCCGAAGATTATATATCAGACAATGAAGTTGAATTT
AGTACACTAGAAACAGATGAACAAATAATTGATGCTTTAGTTAATAGCCC
AATGATCGAAGTTCGTAAAAAACGAAAATCTATTAACTGTTCTTTAGATG
GATCAATTCGTTTCAATCCTTTAAGAGATGATATCCTTGAAACTTCAGCT
GGCTCGTATCATAATTATCAGCAAAATAGGCGTAAAACCATAGCTGTCAT
GAAACGTCTTTCATCTCCATTGAAACGACAGCCTAGAAAACTTAGACAGA
GTATTACTCCTTTGACAACTAAAGAAAACTTGGATCCTACATTAAAAGTA
GAAAAGGAAATAGAATTATTGGTAGCTGCTGTTGACCATTCACAGACATT
ACTGAACATTGAAAAGATTAGGCTCTCGGATTTCATTAAAGATTGGTCTC
ACATATTAGAAAGAGAAACGGATATACCATTAGAATATCAAGGAGACATA
AAGTCAGCCATAGGGGGTTTAAAAATGCTTCTGGAAAATCGGTTTCAATC
GACATTAGATTTATTGGATGCTGCTGAGGCTTATGTGAATAATAATGGGA
AACCGGTAAATGATACAAATCAACACTATCTGTCTGGTGATATCATTGGA
TTCACGGATTTATTGATTGATGAGATCAAAAAAATTGACATTGACTTCAA
TAAACTTTACAATTTGCGAAAAAATAACTGGGTTGAAGATTCAGTTAAAA
CACCCGAAACTATAAAAATTCCTGTTAAGAAAATTAAGAAAGGATTACAA
AAAAAAACTGTTCAGAGTAAAATTAACATGGCCTTGTTGCGCATGAAGTA
CCAAGTGAATAAAGCTAATTCCATAAAATCTCCTGAAATGTTAATTGAAG
AAACTGTCGAAATAGATTCAAATATACCAACAAAAGTTCGACGTTCTAAA
AGACATATTGAGAAACCGGCTGAAGCGGATGAAAACATGGATGAAACAAA
TGAAAATTATAATAAAGATTCAGAGCAAAGAAATGAAACTCGACATTTGT
CAACGTTAGGAAATATCATAAGACCAAAATCTATTTTAAAACGAAAAACT
GGGCGTTCGAGTATTTCTGATAAAACCGATTTAAGATTTGACTTGGAAAA
GAAAAATGATTGTAGTAATGAAAATTCCAGTAAAGATGTGATAAATCTAT
CGAAAACTCCAACTTCACTAAGAAAAGGAATTCGATCAAAATCAATCCTA
AAATCCGACACGGGAACTCCAGTTCATGAAGATACTCCTAGGCCAATCAA
AGTAAGAAAGGAAGCCAAAGAGTTTGCAATTCCATCATCGAGTAGTTTTT
TCCCAAAAAATAAACAGCAAAAGCGGGCTTCACACGTGGTTACTAGTGAA
TACATGAATTCGAAGAAGCCTAGAAAGAGTGTCCGTTAAAGATTATATGA
ACGTTAATTATCGAAATTTATGTTTGCGTATTGTTGTGAGATCGGA
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protein sequence of SMED30024468-orf-1

>SMED30024468-orf-1 ID=SMED30024468-orf-1|Name=SMED30024468-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=520bp
MNKEIHISQVPLTPEGVNHDFNAVKDYEFVRYSEIMKVRTKSMIWSEDYI
SDNEVEFSTLETDEQIIDALVNSPMIEVRKKRKSINCSLDGSIRFNPLRD
DILETSAGSYHNYQQNRRKTIAVMKRLSSPLKRQPRKLRQSITPLTTKEN
LDPTLKVEKEIELLVAAVDHSQTLLNIEKIRLSDFIKDWSHILERETDIP
LEYQGDIKSAIGGLKMLLENRFQSTLDLLDAAEAYVNNNGKPVNDTNQHY
LSGDIIGFTDLLIDEIKKIDIDFNKLYNLRKNNWVEDSVKTPETIKIPVK
KIKKGLQKKTVQSKINMALLRMKYQVNKANSIKSPEMLIEETVEIDSNIP
TKVRRSKRHIEKPAEADENMDETNENYNKDSEQRNETRHLSTLGNIIRPK
SILKRKTGRSSISDKTDLRFDLEKKNDCSNENSSKDVINLSKTPTSLRKG
IRSKSILKSDTGTPVHEDTPRPIKVRKEAKEFAIPSSSSFFPKNKQQKRA
SHVVTSEYMNSKKPRKSVR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000420parapharyngeal region
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR005026SAPAP
Vocabulary: biological process
TermDefinition
GO:0023052signaling
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005026SAPAP familyPFAMPF03359GKAPcoord: 173..314
e-value: 3.4E-12
score: 46.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 354..387
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 432..462
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 487..511
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 354..389
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 427..519
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 464..481