S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30024454 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Human
Match: TYW1 (tRNA-yW synthesizing protein 1 homolog [Source:HGNC Symbol;Acc:HGNC:25598]) HSP 1 Score: 672.159 bits (1733), Expect = 0.000e+0 Identity = 346/647 (53.48%), Postives = 434/647 (67.08%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTCT--SETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEI----TEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMMMR-------------------KRKDIVENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQ------TGKDFDATEYMAPTPSWAAYGSNEKGFNPSDTRFRKLRK 1975
+KI YGS TG +K A ++ + ++N+ YDP+++L +E K++ V V+TY +G P E A+WF WL +A DFR K +L+ +++AV GLG+S Y S+FN V K +D+ L R++ G D +VV SK+G ++ D++ W KF L E K S G C K + + +E+ E+ TEE E + +E ++ S N +VD+EDLG +M + E+ R M+TP LR +L KQGY+LIGSHSGVK+CRWTKSMLRGRGGCYKH FYGI SHRCME TPSLACANKCVFCWRHH+NPVGTEW+W MD PEMI+ EA+ H M+KQFKGVPGV+ ERF EG+ KHCALSLVGEPIMYP IN + LLH +IS+FLVTNAQFP I+ L PVTQLYVSVDA KD+LKKIDRPLFKDFW+RF+ SL A+ K+QRTVYRLTLVKAWNVDEL AYA LV LG P FIEVKGVTYCG+S AS LTMA+VPWHEEV+ FV +L D + YEIA EHEHSNC+L+A +KFK+ EW TWIDY +F EL+Q K F A +YMA TP WA +G++E+GF+P DTR ++ K
Sbjct: 79 VKIFYGSQTGTAKGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWLWMLGAHRVMSRGEGDCDVVKSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSHEQDELHHRDTEEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLKALAVKQQRTVYRLTLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCGESSASSLTMAHVPWHEEVVQFVHELVDLIPEYEIACEHEHSNCLLIAHRKFKIGGEWWTWIDYNRFQELIQEYEDSGGSKTFSAKDYMARTPHWALFGASERGFDPKDTRHQRKNK 725
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Human
Match: TYW1 (tRNA-yW synthesizing protein 1 homolog [Source:HGNC Symbol;Acc:HGNC:25598]) HSP 1 Score: 569.311 bits (1466), Expect = 0.000e+0 Identity = 309/616 (50.16%), Postives = 392/616 (63.64%), Query Frame = 2
Query: 230 MMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTCT--SETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEI----TEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMMMRKRKDIV-------------------ENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQTGKD------FDATEYMAPTPSWAAYGSNEKGFNPSDTRFRKLRK 1975
++N+ YDP+++L +E K++ V V+TY +G P E A+WF WL +A DFR K +L+ +++AV GLG+S Y S+FN V K +D+ L R++ G D +VV SK+G ++ D++ W KF L E K S G C K + + +E+ E+ TEE E + +E ++ S N +VD+EDLG +M K E+ R M+TP LR +L KQ + R R H + SHRCME TPSLACANKCVFCWRHH+NPVGTEW+W MD PEMI+ EA+ H M+KQFKGVPGV+ ERF EG+ KHCALSLVGEPIMYP IN + LLH +IS+FLVTNAQFP I+ L PVTQLYVSVDA KD+LKKIDRPLFKDFW+RF+ SL A+ K+QRTVYRLTLVKAWNVDEL AYA LV LG P FIEVKGVTYCG+S AS LTMA+VPWHEEV+ FV +L D + YEIA EHEHSNC+L+A +KFK+ EW TWIDY +F EL+Q +D F A +YMA TP WA +G++E+GF+P DTR ++ K
Sbjct: 64 IINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWLWMLGAHRVMSRGEGDCDVVKSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSHEQDELHHRDTEEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQVHAP-----------------RERRLLQTHILWN-ESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLKALAVKQQRTVYRLTLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCGESSASSLTMAHVPWHEEVVQFVHELVDLIPEYEIACEHEHSNCLLIAHRKFKIGGEWWTWIDYNRFQELIQEYEDSGGSKTFSAKDYMARTPHWALFGASERGFDPKDTRHQRKNK 661
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Human
Match: TYW1B (tRNA-yW synthesizing protein 1 homolog B [Source:HGNC Symbol;Acc:HGNC:33908]) HSP 1 Score: 550.821 bits (1418), Expect = 0.000e+0 Identity = 307/640 (47.97%), Postives = 394/640 (61.56%), Query Frame = 2
Query: 158 LTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTCT--SETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEI----TEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMMMRKRKDIV-------------------ENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQTGKD------FDATEYMAPTPSWAAYGSNEKGFNPSDTRFRKLRK 1975
+ G + +LAE + + ++N+ YDP+++L +E K++ V V+TY +G P E A+WF WL +A DFR K +L+ ++ AV GLG+S Y S+FN V K +D+ L R++ G D +VV SK+G ++ +++ W KF L E K S G C K + + E+ E+ T+E E + +E ++ S N +VD+EDLG +M K E+ R M+TP LR +L KQ R R H + SHRCME TPSLACANKCVFCW HH+NPVGTEW W MD PEMI+ EA+ H M+KQFKGVPGV+ ERF EG+ KHCALSLVGEPIMYP IN + LLH +IS+FLVTNAQFP I+ L PVTQLYVSVDA KD+LKKIDRPLFKDFW++F+ SL A+ K+QRTVYRL LVKAWNVDEL AYA LV LG P FIEVKGVTYC +S AS LTMA+VPWHEEV+ FV++L D + YEIA EHEHSNC+L+A +KFK+ EW TWIDY +F EL+Q +D F A +YMA TP WA +G+NE+ F+P DTR ++ K
Sbjct: 44 MQGFATVLAEAVTSL----DLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRDAVFGLGNSAYASHFNKVGKNVDKWLWMLGVHRVMSRGEGDCDVVKSKHGSIEANFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSQEQDELHHRDTKEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQ-----------------VDAPRERSLLQTHILWN-ESHRCMETTPSLACANKCVFCWWHHNNPVGTEWLWKMDQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQQFLDSLKALAVKQQRTVYRLMLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCRESSASSLTMAHVPWHEEVVQFVRELVDLIPEYEIACEHEHSNCLLIAHRKFKIGGEWWTWIDYNRFQELIQEYEDSGGSKTFSAKDYMARTPHWALFGANERSFDPKDTRHQRKNK 661
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Human
Match: TYW1B (tRNA-yW synthesizing protein 1 homolog B [Source:HGNC Symbol;Acc:HGNC:33908]) HSP 1 Score: 404.831 bits (1039), Expect = 2.288e-134 Identity = 208/353 (58.92%), Postives = 240/353 (67.99%), Query Frame = 2
Query: 794 SGNELVDMEDLGMMMRKRKDIV-------------------ENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKK 1795
S N +VD+EDLG +M K E+ R M+TP LR +L KQ R R H + SHRCME TPSLACANKCVFCW HH+NPVGTEW W MD PEMI+ EA+ H M+KQFKGVPGV+ ERF EG+ KHCALSLVGEPIMYP IN + LLH +IS+FLVTNAQFP I+ L PVTQLYVSVDA KD+LKKIDRPLFKDFW++F+ SL A+ K+QRTVYRL LVKAWNVDEL AYA LV LG P FIEVKGVTYC +S AS LTMA+VPWHEEV+ FV++L D + YEIA EHEHSNC+L+A +K
Sbjct: 99 SLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQ-----------------VDAPRERSLLQTHILWN-ESHRCMETTPSLACANKCVFCWWHHNNPVGTEWLWKMDQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQQFLDSLKALAVKQQRTVYRLMLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCRESSASSLTMAHVPWHEEVVQFVRELVDLIPEYEIACEHEHSNCLLIAHRK 433
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Human
Match: TYW1 (tRNA-yW synthesizing protein 1 homolog [Source:HGNC Symbol;Acc:HGNC:25598]) HSP 1 Score: 122.865 bits (307), Expect = 1.391e-29 Identity = 94/289 (32.53%), Postives = 144/289 (49.83%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTC--TSETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEI----TEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMMMRKRKDIV-------------------ENKTPREMLTPMLRTSLEKQ 919
+KI YGS TG +K A ++ + ++N+ YDP+++L +E K++ V V+TY +G P E A+WF WL +A DFR K +L+ +++AV GLG+S Y S+FN V K +D+ L R++ G D +VV SK+G ++ D++ W KF L E K S G C K + + +E+ E+ TEE E + +E ++ S N +VD+EDLG +M K E+ R M+TP LR +L KQ
Sbjct: 79 VKIFYGSQTGTAKGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWLWMLGAHRVMSRGEGDCDVVKSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSHEQDELHHRDTEEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQ 367
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Celegans
Match: emb-8 (NADPH--cytochrome P450 reductase [Source:UniProtKB/TrEMBL;Acc:Q09590]) HSP 1 Score: 65.0846 bits (157), Expect = 9.856e-11 Identity = 48/165 (29.09%), Postives = 84/165 (50.91%), Query Frame = 2
Query: 122 NEKNTIKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPE-ENLNK----EKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKF 601
NE + I+YGS TG ++ ++ + +K+ Y K + ++ D E E+LN+ E +LLV+ ++TY G P ++A +L D L ++FAV GLG+ YE +FN + +D+ L KR+ +G+ D+ + L+ D+ W + F
Sbjct: 68 NENRQVLIMYGSQTGTAEEMSGRLAKDL--TRYTKKAVVVDPEDIECEDLNRLSEVEDALLVLCIATYGEGDPTDNAVTLVEYLNAGDCD-------LSGVRFAVFGLGNKTYE-HFNEIGIQMDKQLEKLGAKRIFHLGLGDD------DANLEEDFMIWREAF 216
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Celegans
Match: emb-8 (NADPH--cytochrome P450 reductase [Source:UniProtKB/TrEMBL;Acc:Q09590]) HSP 1 Score: 65.0846 bits (157), Expect = 9.856e-11 Identity = 48/165 (29.09%), Postives = 84/165 (50.91%), Query Frame = 2
Query: 122 NEKNTIKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPE-ENLNK----EKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKF 601
NE + I+YGS TG ++ ++ + +K+ Y K + ++ D E E+LN+ E +LLV+ ++TY G P ++A +L D L ++FAV GLG+ YE +FN + +D+ L KR+ +G+ D+ + L+ D+ W + F
Sbjct: 68 NENRQVLIMYGSQTGTAEEMSGRLAKDL--TRYTKKAVVVDPEDIECEDLNRLSEVEDALLVLCIATYGEGDPTDNAVTLVEYLNAGDCD-------LSGVRFAVFGLGNKTYE-HFNEIGIQMDKQLEKLGAKRIFHLGLGDD------DANLEEDFMIWREAF 216
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Celegans
Match: mtrr-1 (Methionine synthase reductase [Source:UniProtKB/Swiss-Prot;Acc:Q17574]) HSP 1 Score: 62.003 bits (149), Expect = 1.019e-9 Identity = 43/163 (26.38%), Postives = 83/163 (50.92%), Query Frame = 2
Query: 143 ILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKEKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKN-----FLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLT 616
I +GS TG+++ +A+ ++ ++ ++ + + NLN+EK L I VS+ +G P++ F R+++N +L+NL + ++GLG S Y S++ + + ID+ +L RL + D+ V GL+ + + W +KF+ TL
Sbjct: 6 IAFGSQTGQAETIAKSLKEKAELIGLTPRLHALDENEKKFNLNEEK-LCAIVVSSTGDGDAPDNCARFVR---------RINRNSLENEYLKNLDYVLLGLGDSNY-SSYQTIPRKIDKQLTALGANRLFDRAEADDQV------GLELEVEPWIEKFFATLA 151
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Celegans
Match: mtrr-1 (Methionine synthase reductase [Source:UniProtKB/Swiss-Prot;Acc:Q17574]) HSP 1 Score: 62.003 bits (149), Expect = 1.019e-9 Identity = 43/163 (26.38%), Postives = 83/163 (50.92%), Query Frame = 2
Query: 143 ILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKEKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKN-----FLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLT 616
I +GS TG+++ +A+ ++ ++ ++ + + NLN+EK L I VS+ +G P++ F R+++N +L+NL + ++GLG S Y S++ + + ID+ +L RL + D+ V GL+ + + W +KF+ TL
Sbjct: 6 IAFGSQTGQAETIAKSLKEKAELIGLTPRLHALDENEKKFNLNEEK-LCAIVVSSTGDGDAPDNCARFVR---------RINRNSLENEYLKNLDYVLLGLGDSNY-SSYQTIPRKIDKQLTALGANRLFDRAEADDQV------GLELEVEPWIEKFFATLA 151
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Celegans
Match: fre-1 (NADPH-dependent diflavin oxidoreductase 1 [Source:UniProtKB/TrEMBL;Acc:M1ZMI3]) HSP 1 Score: 59.3066 bits (142), Expect = 6.353e-9 Identity = 43/165 (26.06%), Postives = 75/165 (45.45%), Query Frame = 2
Query: 134 TIKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKEKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTCTSE 628
+I ILYGS TG ++ +AE +E + ++ ++ YD + L EK +L + +T + PP K W + + D FL+N+ V+GLG S Y+ +N K + L K + + + D+ G+D + W + WK + +E
Sbjct: 2 SIAILYGSETGTAQDIAESLRREAQQRHLQARVHELDEYDVSQ-LPMEKVVLFVVSTTGQGEMPPNMRK---TWKLLLRKSLGAD--FLKNVNIGVLGLGDSSYQ-KYNFAGKKLYRRLVQLGAKMMCGVHLADDQ----HEIGIDGAFIPWKTECWKKIKEIAE 155
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Fly
Match: Cpr (gene:FBgn0015623 transcript:FBtr0331224) HSP 1 Score: 68.9366 bits (167), Expect = 8.042e-12 Identity = 46/163 (28.22%), Postives = 78/163 (47.85%), Query Frame = 2
Query: 134 TIKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENL---NKEKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL 613
++ + YGS TG + A + +KE I + + D EE L + + SL V ++TY G P ++A FY W+ D L L +AV GLG+ YE ++N V+ +D+ L R+ E+G+ D+ + ++ D+ W +FW +
Sbjct: 83 SLVVFYGSQTGTGEEFAGRLAKEGIRYRLKGMVADPEECDMEELLQLKDIDNSLAVFCLATYGEGDPTDNAMEFYEWITSGDVD-------LSGLNYAVFGLGNKTYE-HYNKVAIYVDKRLEELGANRVFELGLGDD------DANIEDDFITWKDRFWPAV 231
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Fly
Match: Cpr (gene:FBgn0015623 transcript:FBtr0331223) HSP 1 Score: 68.9366 bits (167), Expect = 8.042e-12 Identity = 46/163 (28.22%), Postives = 78/163 (47.85%), Query Frame = 2
Query: 134 TIKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENL---NKEKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL 613
++ + YGS TG + A + +KE I + + D EE L + + SL V ++TY G P ++A FY W+ D L L +AV GLG+ YE ++N V+ +D+ L R+ E+G+ D+ + ++ D+ W +FW +
Sbjct: 83 SLVVFYGSQTGTGEEFAGRLAKEGIRYRLKGMVADPEECDMEELLQLKDIDNSLAVFCLATYGEGDPTDNAMEFYEWITSGDVD-------LSGLNYAVFGLGNKTYE-HYNKVAIYVDKRLEELGANRVFELGLGDD------DANIEDDFITWKDRFWPAV 231
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Fly
Match: Cpr (gene:FBgn0015623 transcript:FBtr0079250) HSP 1 Score: 68.9366 bits (167), Expect = 8.042e-12 Identity = 46/163 (28.22%), Postives = 78/163 (47.85%), Query Frame = 2
Query: 134 TIKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENL---NKEKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL 613
++ + YGS TG + A + +KE I + + D EE L + + SL V ++TY G P ++A FY W+ D L L +AV GLG+ YE ++N V+ +D+ L R+ E+G+ D+ + ++ D+ W +FW +
Sbjct: 83 SLVVFYGSQTGTGEEFAGRLAKEGIRYRLKGMVADPEECDMEELLQLKDIDNSLAVFCLATYGEGDPTDNAMEFYEWITSGDVD-------LSGLNYAVFGLGNKTYE-HYNKVAIYVDKRLEELGANRVFELGLGDD------DANIEDDFITWKDRFWPAV 231
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Fly
Match: CG13667 (gene:FBgn0035890 transcript:FBtr0076611) HSP 1 Score: 63.1586 bits (152), Expect = 5.465e-10 Identity = 47/170 (27.65%), Postives = 82/170 (48.24%), Query Frame = 2
Query: 143 ILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKEKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTCTS---ETKNSN 643
+LYGS TG ++ +AE+ +E + ++ + YD + + E+ L+V V+T +G P++ K + +L+ + L+ +QFA +GLG S Y FN +K + + +L + +G+CD+ G L W+K W L S E+K SN
Sbjct: 5 VLYGSQTGTAQDVAEQIWRESHQLGFQGPVLPFDEYDMTKLI--EERLVVFVVATTGDGVEPDNMKLAWRFLLKRS----LPAQSLQGMQFACLGLGDSSY-PKFNYAAKKLSKRLQNLGANSVCPVGLCDD---QHDYGHLGVSL-SWTKDLWTALKGISGLDESKLSN 163
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Fly
Match: CG13667 (gene:FBgn0035890 transcript:FBtr0076610) HSP 1 Score: 63.1586 bits (152), Expect = 5.465e-10 Identity = 47/170 (27.65%), Postives = 82/170 (48.24%), Query Frame = 2
Query: 143 ILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKEKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTCTS---ETKNSN 643
+LYGS TG ++ +AE+ +E + ++ + YD + + E+ L+V V+T +G P++ K + +L+ + L+ +QFA +GLG S Y FN +K + + +L + +G+CD+ G L W+K W L S E+K SN
Sbjct: 5 VLYGSQTGTAQDVAEQIWRESHQLGFQGPVLPFDEYDMTKLI--EERLVVFVVATTGDGVEPDNMKLAWRFLLKRS----LPAQSLQGMQFACLGLGDSSY-PKFNYAAKKLSKRLQNLGANSVCPVGLCDD---QHDYGHLGVSL-SWTKDLWTALKGISGLDESKLSN 163
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Zebrafish
Match: tyw1 (tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) [Source:NCBI gene;Acc:562076]) HSP 1 Score: 704.131 bits (1816), Expect = 0.000e+0 Identity = 354/646 (54.80%), Postives = 446/646 (69.04%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTCTS--ETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMM---MRKRKDIVE-------------------NKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQT------GKDFDATEYMAPTPSWAAYGSNEKGFNPSDTRFRKLRK 1975
+K+ YGS TG +K A++ +++ I ++++M +DPE+ L +E K + V V+TY +G P E A+WF WL +A DFR K +L+ +++AV GLG+S Y ++N VSK+ID+ LS R++ G D NVV S++G + D+Q W KF L + E K +G+ + K + +EE + + E G+ ++DMEDLG + M+K K +E + REM+TP LR SL KQGYKLIGSHSGVK+CRWTKSMLRGRGGCYKH FYGI SHRCME TPSLACANKCVFCWRHH+NPVGTEW+W MDP E I+ EA+ H M++QF+GVPGV+ ERF EGL KHCALSLVGEPIMYP IN+ + LLH + IS+FLVTNAQFP+ I+ LVPVTQLYVSVDA KD+LKKIDRPLFKDFW+RF+ SL A+ +K+QRTVYRLTLVKAWNVDEL AYA+L+ LGQP FIEVKGVTYCG+S AS LTMANVPWHEEV+ FVQQLA+ L +YEIA EHEHSNC+LLA KFKV+ EW TWIDYE+F EL+Q K+F A +YMA TPSWA +G+ E+GF+P+DTRF++ K
Sbjct: 103 VKVFYGSQTGTAKGFAKELAEDVIAQGIQCEVIDMKDFDPEDRLAEECTSKIICVFLVATYTDGQPTESAEWFCKWLQEASTDFRYGKTYLKGMRYAVFGLGNSVYVGHYNTVSKSIDKWLWMLSAARIMTRGEGDCNVVKSRHGSVQADFQAWKGKFLNRLQALAKGEKKACSGNCKKASCKNKKKHKEEAEDNHSLAEKNNSEEELMESSSDEESSSEDEKSHGS-VIDMEDLGNVMNHMKKAKQRMEEDEEDSQRVKQNGERKSECEEERREMITPALRDSLTKQGYKLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDPAEKIIQEAMENHRNMIRQFRGVPGVRPERFEEGLTVKHCALSLVGEPIMYPEINSFLKLLHQQNISSFLVTNAQFPEEIRSLVPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLRALGEKQQRTVYRLTLVKAWNVDELKAYADLIALGQPDFIEVKGVTYCGESSASSLTMANVPWHEEVIYFVQQLANLLPDYEIACEHEHSNCLLLANHKFKVDGEWWTWIDYERFQELIQQYEESGGTKNFSAMDYMAKTPSWAVFGAGERGFDPTDTRFQRKNK 747
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Zebrafish
Match: pora (P450 (cytochrome) oxidoreductase a [Source:ZFIN;Acc:ZDB-GENE-050809-121]) HSP 1 Score: 82.0333 bits (201), Expect = 9.855e-16 Identity = 54/168 (32.14%), Postives = 85/168 (50.60%), Query Frame = 2
Query: 134 TIKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE--------KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL 613
++ + YGS TG + A + SK+ + Y K M DPEE E KS+ V ++TY G P ++A+ FY+WL DD LE + FAV GLG+ YE ++N + K D+ L GKR+ ++G+ D+ + L+ D+ W ++FW +
Sbjct: 76 SVVVFYGSQTGTGEEFANRLSKDA--HRYGMKGM---AADPEEYDMSELSRLKEIPKSMAVFCMATYGEGDPTDNAQEFYDWLQGTDDD-------LEGVNFAVFGLGNKTYE-HYNAMGKYTDKRLAELGGKRVFDLGLGDD------DSNLEEDFISWKEQFWPAV 224
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Zebrafish
Match: porb (P450 (cytochrome) oxidoreductase b [Source:ZFIN;Acc:ZDB-GENE-030131-5767]) HSP 1 Score: 75.8702 bits (185), Expect = 8.664e-14 Identity = 50/167 (29.94%), Postives = 86/167 (51.50%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEE----NLNK----EKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL 613
I + YGS TG AE+FS + + + M M DPEE L++ + SL + ++TY G P ++A+ FY+WL +A DF L++ V LG+ YE ++N + K +D+ L +R+ ++G+ D+ +G L+ D+ W ++FW +
Sbjct: 74 IVVFYGSQTGT----AEEFSNRLAKDAHRYGMKGMAA-DPEEYDLSELSRMGEIDNSLAIFCLATYGEGDPTDNAQDFYDWLQEADADF-------SGLKYTVFALGNKTYE-HYNAMGKYVDKRLEELGAQRIFDLGMGDD------DGNLEEDFVSWREQFWPAV 221
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Zebrafish
Match: ndor1 (NADPH dependent diflavin oxidoreductase 1 [Source:NCBI gene;Acc:393621]) HSP 1 Score: 70.4774 bits (171), Expect = 4.129e-12 Identity = 53/165 (32.12%), Postives = 82/165 (49.70%), Query Frame = 2
Query: 131 NTIKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKEKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFR--VDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFW-KTLT 616
+T+ +LYGS TG ++ AE+ ++ K ++TY+ NL E SL+V +T G PP++ K F+ +L FR + + L L AV+GLG S Y FN V+K + + L LL +G+ D+ +G +D W FW KTL+
Sbjct: 4 HTVLVLYGSQTGTAQDTAERIGRQAQRRRLRVKAEALDTYN-VANLISE-SLVVFVCATTGQGDPPDNMKKFWRFL------FRKSLPADSLSRLDCAVLGLGDSSY-PKFNFVAKKLHKRLLQLGANMLLPVGLADDQHDLGPDGVID----PWLLSFWQKTLS 155
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Zebrafish
Match: ndor1 (NADPH dependent diflavin oxidoreductase 1 [Source:NCBI gene;Acc:393621]) HSP 1 Score: 70.4774 bits (171), Expect = 4.129e-12 Identity = 53/165 (32.12%), Postives = 82/165 (49.70%), Query Frame = 2
Query: 131 NTIKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKEKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFR--VDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFW-KTLT 616
+T+ +LYGS TG ++ AE+ ++ K ++TY+ NL E SL+V +T G PP++ K F+ +L FR + + L L AV+GLG S Y FN V+K + + L LL +G+ D+ +G +D W FW KTL+
Sbjct: 4 HTVLVLYGSQTGTAQDTAERIGRQAQRRRLRVKAEALDTYN-VANLISE-SLVVFVCATTGQGDPPDNMKKFWRFL------FRKSLPADSLSRLDCAVLGLGDSSY-PKFNFVAKKLHKRLLQLGANMLLPVGLADDQHDLGPDGVID----PWLLSFWQKTLS 155
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Xenopus
Match: large2 (LARGE xylosyl- and glucuronyltransferase 2 [Source:Xenbase;Acc:XB-GENE-5871953]) HSP 1 Score: 697.197 bits (1798), Expect = 0.000e+0 Identity = 348/645 (53.95%), Postives = 450/645 (69.77%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL-TCTSETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEEKNMDSGNELVDMEDLG---MMMRKRK-------------------DIVENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQT------GKDFDATEYMAPTPSWAAYGSNEKGFNPSDTRFRKLRK 1975
+KI YGS TG +K A E +++NM YDP++NL +E K++ V V+TY +G PPE A+WF WL +A +DFR K +L+ +++AV GLG+S Y +++N V K ID+ LS R++ D NVV SK+G ++ D++ W +F L S K C K +++++ + + E E + TSD E E++ + G+ L+DMEDLG +M+K K + E + PREM+TP L+ +L KQGY+LIGSHSGVK+CRWTKSMLRGRGGCYKH FYGI SHRCME TPSLACANKCVFCWRHH+NPVGTEW+W MD PEMI++EA+ H M+KQFKGVPGV+ ER+ EGL KHCALSLVGEPIMYP IN + LLH++ IS+FLVTNAQFP+ I+ L PVTQLYVSVDA KD+LKKIDRPLFKDFW+RF+ SL A+ +K+QRTVYRLTLVKAWNV+EL AYA+LV +G+P FIEVKGVTYCG+S AS LTMANVPWH+EV+ FV++LAD L +YE+A EHEHSNC+L+A +KFK+N +W TWIDYE+F EL++ + F A +Y+A TP WA +GS E+GF+P D RF++ K
Sbjct: 84 VKIFYGSQTGTAKGFAHMLDGEITLLGLPVEIINMKEYDPDDNLIEEATSKNVCVFLVATYTDGQPPESAEWFCKWLEEASNDFRFGKTYLKGMRYAVFGLGNSVYSTHYNTVGKNIDKWLWMLSASRVMTRAEGDCNVVKSKHGSIESDFEAWKARFLSRLKALISGEKKPCSGKCKKGKCKSKMKSSTDAVEEEAKHSEHEETEEETFETTSDSEPEEHGEPGSGLIDMEDLGKAMSIMKKAKREQDTNEEARNRGKSLQSDEAKEKEEPREMITPALQEALTKQGYRLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILEEAILNHQNMIKQFKGVPGVKPERYEEGLVVKHCALSLVGEPIMYPEINKFLRLLHNQHISSFLVTNAQFPEEIRSLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLKALAEKQQRTVYRLTLVKAWNVEELKAYADLVAVGRPDFIEVKGVTYCGESSASNLTMANVPWHDEVVRFVEELADLLPDYEVACEHEHSNCMLIAHEKFKINGDWWTWIDYERFQELIRAFDESKGSQTFTAMDYVAKTPEWALFGSRERGFDPLDKRFQRKNK 728
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Xenopus
Match: large2 (LARGE xylosyl- and glucuronyltransferase 2 [Source:Xenbase;Acc:XB-GENE-5871953]) HSP 1 Score: 697.197 bits (1798), Expect = 0.000e+0 Identity = 348/645 (53.95%), Postives = 450/645 (69.77%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL-TCTSETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEEKNMDSGNELVDMEDLG---MMMRKRK-------------------DIVENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQT------GKDFDATEYMAPTPSWAAYGSNEKGFNPSDTRFRKLRK 1975
+KI YGS TG +K A E +++NM YDP++NL +E K++ V V+TY +G PPE A+WF WL +A +DFR K +L+ +++AV GLG+S Y +++N V K ID+ LS R++ D NVV SK+G ++ D++ W +F L S K C K +++++ + + E E + TSD E E++ + G+ L+DMEDLG +M+K K + E + PREM+TP L+ +L KQGY+LIGSHSGVK+CRWTKSMLRGRGGCYKH FYGI SHRCME TPSLACANKCVFCWRHH+NPVGTEW+W MD PEMI++EA+ H M+KQFKGVPGV+ ER+ EGL KHCALSLVGEPIMYP IN + LLH++ IS+FLVTNAQFP+ I+ L PVTQLYVSVDA KD+LKKIDRPLFKDFW+RF+ SL A+ +K+QRTVYRLTLVKAWNV+EL AYA+LV +G+P FIEVKGVTYCG+S AS LTMANVPWH+EV+ FV++LAD L +YE+A EHEHSNC+L+A +KFK+N +W TWIDYE+F EL++ + F A +Y+A TP WA +GS E+GF+P D RF++ K
Sbjct: 105 VKIFYGSQTGTAKGFAHMLDGEITLLGLPVEIINMKEYDPDDNLIEEATSKNVCVFLVATYTDGQPPESAEWFCKWLEEASNDFRFGKTYLKGMRYAVFGLGNSVYSTHYNTVGKNIDKWLWMLSASRVMTRAEGDCNVVKSKHGSIESDFEAWKARFLSRLKALISGEKKPCSGKCKKGKCKSKMKSSTDAVEEEAKHSEHEETEEETFETTSDSEPEEHGEPGSGLIDMEDLGKAMSIMKKAKREQDTNEEARNRGKSLQSDEAKEKEEPREMITPALQEALTKQGYRLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILEEAILNHQNMIKQFKGVPGVKPERYEEGLVVKHCALSLVGEPIMYPEINKFLRLLHNQHISSFLVTNAQFPEEIRSLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLKALAEKQQRTVYRLTLVKAWNVEELKAYADLVAVGRPDFIEVKGVTYCGESSASNLTMANVPWHDEVVRFVEELADLLPDYEVACEHEHSNCMLIAHEKFKINGDWWTWIDYERFQELIRAFDESKGSQTFTAMDYVAKTPEWALFGSRERGFDPLDKRFQRKNK 749
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Xenopus
Match: large2 (LARGE xylosyl- and glucuronyltransferase 2 [Source:Xenbase;Acc:XB-GENE-5871953]) HSP 1 Score: 674.085 bits (1738), Expect = 0.000e+0 Identity = 338/626 (53.99%), Postives = 434/626 (69.33%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTC--TSETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEEKNMDSGNELVDMEDLG--MMMRKRKDIVENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQT------GKDFDATEYMAPTPSWAAYGSNEKGFNPSDTRFRKLRK 1975
+KI YGS TG +K A E +++NM YDP++NL +E K++ V V+TY +G PPE A+WF WL +A +DFR K +L+ +++AV GLG+S Y +++N V K ID+ LS R++ D NVV SK+G ++ D++ W +F L + E K + K++K+E E TSD E E++ + G+ L+DMEDLG MM+ K + +L + GY+LIGSHSGVK+CRWTKSMLRGRGGCYKH FYGI SHRCME TPSLACANKCVFCWRHH+NPVGTEW+W MD PEMI++EA+ H M+KQFKGVPGV+ ER+ EGL KHCALSLVGEPIMYP IN + LLH++ IS+FLVTNAQFP+ I+ L PVTQLYVSVDA KD+LKKIDRPLFKDFW+RF+ SL A+ +K+QRTVYRLTLVKAWNV+EL AYA+LV +G+P FIEVKGVTYCG+S AS LTMANVPWH+EV+ FV++LAD L +YE+A EHEHSNC+L+A +KFK+N +W TWIDYE+F EL++ + F A +Y+A TP WA +GS E+GF+P D RF++ K
Sbjct: 48 VKIFYGSQTGTAKGFAHMLDGEITLLGLPVEIINMKEYDPDDNLIEEATSKNVCVFLVATYTDGQPPESAEWFCKWLEEASNDFRFGKTYLKGMRYAVFGLGNSVYSTHYNTVGKNIDKWLWMLSASRVMTRAEGDCNVVKSKHGSIESDFEAWKARFLSRLKALISGEKKPCS----------------GKCKKGKSKKVETFET-------TSDSEPEEHGEPGSGLIDMEDLGKAMMLNKYFTVT-----------VLNLLCKFSGYRLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILEEAILNHQNMIKQFKGVPGVKPERYEEGLVVKHCALSLVGEPIMYPEINKFLRLLHNQHISSFLVTNAQFPEEIRSLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLKALAEKQQRTVYRLTLVKAWNVEELKAYADLVAVGRPDFIEVKGVTYCGESSASNLTMANVPWHDEVVRFVEELADLLPDYEVACEHEHSNCMLIAHEKFKINGDWWTWIDYERFQELIRAFDESKGSQTFTAMDYVAKTPEWALFGSRERGFDPLDKRFQRKNK 639
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Xenopus
Match: large2 (LARGE xylosyl- and glucuronyltransferase 2 [Source:Xenbase;Acc:XB-GENE-5871953]) HSP 1 Score: 673.7 bits (1737), Expect = 0.000e+0 Identity = 337/623 (54.09%), Postives = 436/623 (69.98%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL-TCTSETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMMMRKRKDIVENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQT------GKDFDATEYMAPTPSWAAYGSNEKGFNPSDTRFRKLRK 1975
+KI YGS TG +K A E +++NM YDP++NL +E K++ V V+TY +G PPE A+WF WL +A +DFR K +L+ +++AV GLG+S Y +++N V K ID+ LS R++ D NVV SK+G ++ D++ W +F L S K C K +++++ + + E E + TSD E E++ + G+ L+DMEDLG + M+T +L + GY+LIGSHSGVK+CRWTKSMLRGRGGCYKH FYGI SHRCME TPSLACANKCVFCWRHH+NPVGTEW+W MD PEMI++EA+ H M+KQFKGVPGV+ ER+ EGL KHCALSLVGEPIMYP IN + LLH++ IS+FLVTNAQFP+ I+ L PVTQLYVSVDA KD+LKKIDRPLFKDFW+RF+ SL A+ +K+QRTVYRLTLVKAWNV+EL AYA+LV +G+P FIEVKGVTYCG+S AS LTMANVPWH+EV+ FV++LAD L +YE+A EHEHSNC+L+A +KFK+N +W TWIDYE+F EL++ + F A +Y+A TP WA +GS E+GF+P D RF++ K
Sbjct: 68 VKIFYGSQTGTAKGFAHMLDGEITLLGLPVEIINMKEYDPDDNLIEEATSKNVCVFLVATYTDGQPPESAEWFCKWLEEASNDFRFGKTYLKGMRYAVFGLGNSVYSTHYNTVGKNIDKWLWMLSASRVMTRAEGDCNVVKSKHGSIESDFEAWKARFLSRLKALISGEKKPCSGKCKKGKCKSKMKSSTDAVEEEAKHSEHEETEEETFETTSDSEPEEHGEPGSGLIDMEDLG---------------KAMIT-VLNLLCKFSGYRLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILEEAILNHQNMIKQFKGVPGVKPERYEEGLVVKHCALSLVGEPIMYPEINKFLRLLHNQHISSFLVTNAQFPEEIRSLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLKALAEKQQRTVYRLTLVKAWNVEELKAYADLVAVGRPDFIEVKGVTYCGESSASNLTMANVPWHDEVVRFVEELADLLPDYEVACEHEHSNCMLIAHEKFKINGDWWTWIDYERFQELIRAFDESKGSQTFTAMDYVAKTPEWALFGSRERGFDPLDKRFQRKNK 674
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Xenopus
Match: large2 (LARGE xylosyl- and glucuronyltransferase 2 [Source:Xenbase;Acc:XB-GENE-5871953]) HSP 1 Score: 608.986 bits (1569), Expect = 0.000e+0 Identity = 304/573 (53.05%), Postives = 397/573 (69.28%), Query Frame = 2
Query: 278 KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL-TCTSETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMMMRKRKDIVENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQT------GKDFDATEYMAPTPSWAAYGSNEKGFNPSDTRFRKLRK 1975
K++ V V+TY +G PPE A+WF WL +A +DFR K +L+ +++AV GLG+S Y +++N V K ID+ LS R++ D NVV SK+G ++ D++ W +F L S K C K +++++ + + E E + TSD E E++ + G+ L+DMEDLG M S+ K+ + S + + +++SMLRGRGGCYKH FYGI SHRCME TPSLACANKCVFCWRHH+NPVGTEW+W MD PEMI++EA+ H M+KQFKGVPGV+ ER+ EGL KHCALSLVGEPIMYP IN + LLH++ IS+FLVTNAQFP+ I+ L PVTQLYVSVDA KD+LKKIDRPLFKDFW+RF+ SL A+ +K+QRTVYRLTLVKAWNV+EL AYA+LV +G+P FIEVKGVTYCG+S AS LTMANVPWH+EV+ FV++LAD L +YE+A EHEHSNC+L+A +KFK+N +W TWIDYE+F EL++ + F A +Y+A TP WA +GS E+GF+P D RF++ K
Sbjct: 8 KNVCVFLVATYTDGQPPESAEWFCKWLEEASNDFRFGKTYLKGMRYAVFGLGNSVYSTHYNTVGKNIDKWLWMLSASRVMTRAEGDCNVVKSKHGSIESDFEAWKARFLSRLKALISGEKKPCSGKCKKGKCKSKMKSSTDAVEEEAKHSEHEETEEETFETTSDSEPEEHGEPGSGLIDMEDLGKAM----------------------SIMKKAKNMNLSFIHIYVFLFSQSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILEEAILNHQNMIKQFKGVPGVKPERYEEGLVVKHCALSLVGEPIMYPEINKFLRLLHNQHISSFLVTNAQFPEEIRSLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLKALAEKQQRTVYRLTLVKAWNVEELKAYADLVAVGRPDFIEVKGVTYCGESSASNLTMANVPWHDEVVRFVEELADLLPDYEVACEHEHSNCMLIAHEKFKINGDWWTWIDYERFQELIRAFDESKGSQTFTAMDYVAKTPEWALFGSRERGFDPLDKRFQRKNK 558
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Mouse
Match: Tyw1 (tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) [Source:MGI Symbol;Acc:MGI:2141161]) HSP 1 Score: 671.389 bits (1731), Expect = 0.000e+0 Identity = 341/650 (52.46%), Postives = 433/650 (66.62%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL---------TCTSETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMMM-------------------RKRKDIVENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELV----QTG--KDFDATEYMAPTPSWAAYGSNEKGFNPSDTRFRKLRK 1975
+KI YGS TG +K A +K + ++N+ YDP+++L E K++ V+TY +G P E A+WF WL ++ +DFR K +L+ L++AV GLG S Y S+FN VS +D+ L +R+L G D N V SK+G ++ D+ W KF L C K + + E+ E E +S+DE+ T D + S +VD+EDLG +M ++ + E+ R M+TP LR +L KQGY+LIGSHSGVK+CRWTKSMLRGRGGCYKH FYGI SHRCMEATPSLACANKCVFCWRHH+NPVGTEW+W MD PE+I+ EA+ H M+KQFKGVPG++ ERF EG++ KHCALSLVGEPIMYP IN L+ LLH IS+FLVTNAQFP+ I++L PVTQLYVSVDA +D LKKIDRPLFKDFW+RF+ SL A+ K+QRTVYRLTLVK WNVDEL AYA LV LG P FIEVKGVTYCG+S AS LTMANVPWHEEV+ FV++L D L +YE+A EHEHSNC+L+ KKFK++ EW TWI+Y +F ELV ++G K F + +YMA TP WA +G+ E+GF+P DTR+++ K
Sbjct: 71 VKIFYGSQTGTAKGFAVVLAKAVTSLDLPVAIINLKEYDPDDSLIGEITSKTVCAFLVATYTDGCPTESAEWFCKWLEESANDFRFGKTYLKGLRYAVFGLGDSAYRSHFNKVSTNVDKWLWMLGAQRVLTRGEGDCNAVQSKHGSIEADFTAWKTKFISRLQALQRGEKKACGGNCKRGKCESAQHGPGEARPHPQGELHPGDAEEEEPCESSSEDELGTQDYQ------SLTSVVDVEDLGNIMNPVKREKREKSHQDGKAAMQRNPEKTEDGEGRAMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGTEWRWKMDQPELILKEAIENHQNMIKQFKGVPGLKAERFEEGMEVKHCALSLVGEPIMYPEINRLLKLLHQHGISSFLVTNAQFPEEIRKLTPVTQLYVSVDASTRDGLKKIDRPLFKDFWQRFLDSLKALSAKQQRTVYRLTLVKCWNVDELQAYAELVSLGNPDFIEVKGVTYCGESAASSLTMANVPWHEEVVRFVRELVDLLPDYEVACEHEHSNCLLIGHKKFKIDGEWWTWINYSRFQELVLQYEESGGSKTFSSRDYMARTPQWALFGARERGFDPKDTRYQRKNK 714
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Mouse
Match: Tyw1 (tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) [Source:MGI Symbol;Acc:MGI:2141161]) HSP 1 Score: 160.999 bits (406), Expect = 1.590e-42 Identity = 106/313 (33.87%), Postives = 155/313 (49.52%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL---------TCTSETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEEKNMDSGNELVDMEDLG-------------------MMMRKRKDIVENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSML 982
+KI YGS TG +K A +K + ++N+ YDP+++L E K++ V+TY +G P E A+WF WL ++ +DFR K +L+ L++AV GLG S Y S+FN VS +D+ L +R+L G D N V SK+G ++ D+ W KF L C K + + E+ E E +S+DE+ T D + S +VD+EDLG M++ + E+ R M+TP LR +L KQGY+LIGSHSGVK+CRWTK +
Sbjct: 71 VKIFYGSQTGTAKGFAVVLAKAVTSLDLPVAIINLKEYDPDDSLIGEITSKTVCAFLVATYTDGCPTESAEWFCKWLEESANDFRFGKTYLKGLRYAVFGLGDSAYRSHFNKVSTNVDKWLWMLGAQRVLTRGEGDCNAVQSKHGSIEADFTAWKTKFISRLQALQRGEKKACGGNCKRGKCESAQHGPGEARPHPQGELHPGDAEEEEPCESSSEDELGTQDYQ------SLTSVVDVEDLGNIMNPVKREKREKSHQDGKAAMQRNPEKTEDGEGRAMITPALREALTKQGYQLIGSHSGVKLCRWTKVFI 377
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Mouse
Match: Tyw1 (tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) [Source:MGI Symbol;Acc:MGI:2141161]) HSP 1 Score: 122.094 bits (305), Expect = 7.509e-31 Identity = 61/158 (38.61%), Postives = 92/158 (58.23%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKF 601
+KI YGS TG +K A +K + ++N+ YDP+++L E K++ V+TY +G P E A+WF WL ++ +DFR K +L+ L++AV GLG S Y S+FN VS +D+ L +R+L G D N V SK+G ++ D+ W KF
Sbjct: 48 VKIFYGSQTGTAKGFAVVLAKAVTSLDLPVAIINLKEYDPDDSLIGEITSKTVCAFLVATYTDGCPTESAEWFCKWLEESANDFRFGKTYLKGLRYAVFGLGDSAYRSHFNKVSTNVDKWLWMLGAQRVLTRGEGDCNAVQSKHGSIEADFTAWKTKF 205
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Mouse
Match: Tyw1 (tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) [Source:MGI Symbol;Acc:MGI:2141161]) HSP 1 Score: 123.25 bits (308), Expect = 7.432e-30 Identity = 61/158 (38.61%), Postives = 92/158 (58.23%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKF 601
+KI YGS TG +K A +K + ++N+ YDP+++L E K++ V+TY +G P E A+WF WL ++ +DFR K +L+ L++AV GLG S Y S+FN VS +D+ L +R+L G D N V SK+G ++ D+ W KF
Sbjct: 71 VKIFYGSQTGTAKGFAVVLAKAVTSLDLPVAIINLKEYDPDDSLIGEITSKTVCAFLVATYTDGCPTESAEWFCKWLEESANDFRFGKTYLKGLRYAVFGLGDSAYRSHFNKVSTNVDKWLWMLGAQRVLTRGEGDCNAVQSKHGSIEADFTAWKTKF 228
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Mouse
Match: Por (P450 (cytochrome) oxidoreductase [Source:MGI Symbol;Acc:MGI:97744]) HSP 1 Score: 82.4185 bits (202), Expect = 6.659e-17 Identity = 54/167 (32.34%), Postives = 91/167 (54.49%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEE----NLNK----EKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL 613
I + YGS TG ++ A + SK+ + + M M+ DPEE +L+ +KSL+V ++TY G P ++A+ FY+WL + D L ++FAV GLG+ YE +FN + K +D+ L +R+ E+G+ D+ +G L+ D+ W ++FW +
Sbjct: 80 IIVFYGSQTGTAEEFANRLSKDA----HRYGMRGMSA-DPEEYDLADLSSLPEIDKSLVVFCMATYGEGDPTDNAQDFYDWLQETDVD-------LTGVKFAVFGLGNKTYE-HFNAMGKYVDQRLEQLGAQRIFELGLGDD------DGNLEEDFITWREQFWPAV 227
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. UniProt/SwissProt
Match: sp|Q2KHP8|TYW1_XENLA (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Xenopus laevis OX=8355 GN=tyw1 PE=2 SV=1) HSP 1 Score: 713.761 bits (1841), Expect = 0.000e+0 Identity = 356/646 (55.11%), Postives = 451/646 (69.81%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFW---KTLTCTSETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVL--TSDEEEEKNMDSGNELVDMEDLGMMMRKRK-------------------DIVENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQT------GKDFDATEYMAPTPSWAAYGSNEKGFNPSDTRFRKLRK 1975
+KI YGS TG SK A ++E +++NM YDP++NL +E K++ V V+TY +G PPE A+WF WL +A +DFR K +L+ +++AV GLG+S Y +++N V K ID+ LS R++ D NVV SK+G ++ D++ W +KF K L + S KS + S + E+ E+++D+ +SD E E++ + G+ L+D+EDLG M K + E + PREM+TP L+ +L KQGY+LIGSHSGVK+CRWTKSMLRGRGGCYKH FYGI SHRCME TPSLACANKCVFCWRHH+NPVGTEW+W MD PEMI++EA+ H M+KQFKGVPGV+ ERF EGL KHCALSLVGEPIMYP INT + LLH++ IS+FLVTNAQFP+ I+ L PVTQLYVSVDA KD+LKKIDRPLFKDFW+RF+ SL A+ +K+QRTVYRLTLVKAWNVDEL AYA+LV LG P FIEVKGVTYCG+S AS LTMANVPWHEEV+ FV++L D L +YE+A EHEHSNC+L+A KKFK+N EW TWIDYE+F EL+Q + F A +Y+A TP WA +GS E+GF+P D RF++ K
Sbjct: 83 VKIFYGSQTGTSKGFAHLLAEEVTLLGLPVELINMKEYDPDDNLVEETTSKNICVFLVATYTDGKPPESAEWFCKWLEEASNDFRFGKTYLKGMRYAVFGLGNSVYSTHYNTVGKNIDKWLWMLSANRVMTRAEGDCNVVKSKHGSIESDFEAWKRKFLNRLKALLSGEKKPCSGKCKKGKCKSKKKSSIESVEEEEEEEEKHSEHEDTEDDTFETSSDSEPEEHGEPGSGLIDVEDLGKAMSNMKKSKREHDSNTELGKSLQGDEAKEKEEPREMITPALQEALTKQGYRLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILEEAILNHQNMIKQFKGVPGVKPERFEEGLAVKHCALSLVGEPIMYPEINTFLRLLHNQHISSFLVTNAQFPEEIRSLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFIDSLKALAEKQQRTVYRLTLVKAWNVDELKAYADLVALGLPDFIEVKGVTYCGESSASNLTMANVPWHEEVIRFVEELVDLLPDYEVACEHEHSNCMLIAHKKFKINGEWCTWIDYERFQELIQAFGESKGSRTFTALDYVAKTPEWALFGSRERGFDPLDKRFQRKNK 728
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. UniProt/SwissProt
Match: sp|Q08C92|TYW1_DANRE (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Danio rerio OX=7955 GN=tyw1 PE=2 SV=1) HSP 1 Score: 699.508 bits (1804), Expect = 0.000e+0 Identity = 354/646 (54.80%), Postives = 445/646 (68.89%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTCTS--ETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMM---MRKRKDIVE-------------------NKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQT------GKDFDATEYMAPTPSWAAYGSNEKGFNPSDTRFRKLRK 1975
+K+ YGS TG +K A++ +++ I ++++M +DPE+ L +E K + V V+TY +G P E A+WF WL +A DFR K +L+ +++AV GLG+S Y +FN VSK+ID+ LS R++ G D NVV S++G + D+Q W KF L + E K +G+ + K + +EE + + E G+ ++DMEDLG + M+K K +E + REM+TP LR SL KQGYKLIGSHSGVK+ RWTKSMLRGRGGCYKH FYGI SHRCME TPSLACANKCVFCWRHH+NPVGTEW+W MDP E I+ EA+ H M++QF+GVPGV+ ERF EGL KHCALSLVGEPIMYP IN+ + LLH + IS+FLVTNAQFP+ I+ LVPVTQLYVSVDA KD+LKKIDRPLFKDFW+RF+ SL A+ +K+QRTVYRLTLVKAWNVDEL AYA+L+ LGQP FIEVKGVTYCG+S AS LTMANVPWHEEV+ FVQQLA+ L +YEIA EHEHSNC+LLA KFKV+ EW TWIDYE+F EL+Q K+F A +YMA TPSWA +G+ E+GF+P+DTRF++ K
Sbjct: 79 VKVFYGSQTGTAKGFAKELAEDVIAQGIQCEVIDMKDFDPEDRLAEECTSKIICVFLVATYTDGQPTESAEWFCKWLEEASTDFRYGKTYLKGMRYAVFGLGNSVYVGHFNTVSKSIDKWLWMLSAARIMTRGEGDCNVVKSRHGSVQADFQVWKGKFLNRLQALAKGEKKACSGNCKKASCKNKKKHKEEAEDNHSLAEKNNSEEELMESSSDEESSSEDEKSHGS-VIDMEDLGNVMNHMKKAKQRMEEDEEDSQRVKQNGERKSECEEERREMITPALRDSLTKQGYKLIGSHSGVKLRRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDPAEKIIQEAMENHRNMIRQFRGVPGVRPERFEEGLTVKHCALSLVGEPIMYPEINSFLKLLHQQNISSFLVTNAQFPEEIRSLVPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLRALGEKQQRTVYRLTLVKAWNVDELKAYADLIALGQPDFIEVKGVTYCGESSASSLTMANVPWHEEVIYFVQQLANLLPDYEIACEHEHSNCLLLANHKFKVDGEWWTWIDYERFQELIQQYEESGGTKNFSAMDYMAKTPSWAVFGAGERGFDPTDTRFQRKNK 723
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. UniProt/SwissProt
Match: sp|Q5REF9|TYW1_PONAB (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Pongo abelii OX=9601 GN=TYW1 PE=2 SV=1) HSP 1 Score: 675.241 bits (1741), Expect = 0.000e+0 Identity = 347/647 (53.63%), Postives = 434/647 (67.08%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTCT--SETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEE----KNMDSGNELVDMEDLGMMMR-------------------KRKDIVENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQ------TGKDFDATEYMAPTPSWAAYGSNEKGFNPSDTRFRKLRK 1975
+KI YGS TG +K A ++ + ++N+ YDP+++L +E K++ V V+TY +G P E A+WF WL +A DFR K +L+ +++AV GLG+S Y S+FN V K +D+ L R++ G D +VV SK+G ++ D++ W KF L E K S G C K + + +E+ E+ ++++E EE K+ S N +VD+EDLG +M + E+ R M+TP LR +L KQGY+LIGSHSGVK+CRWTKSMLRGRGGCYKH FYGI SHRCME TPSLACANKCVFCWRHH+NPVGTEW+W MD PEMI+ EA+ H M+KQFKGVPGV+ ERF EG+ KHCALSLVGEPIMYP IN + LLH +IS+FLVTNAQFP I+ L PVTQLYVSVDA KD+LKKIDRPLFKDFW RF+ SL A+ K+QRTVYRLTLVKAWNVDEL AYA LV LG P FIEVKGVTYCG+S AS LTMA+VPWHEEV+ FV +L D + +YEIA EHEHSNC+L+A KKFK+ EW TWIDY +F EL+Q K F A +YMA TP WA +G+NE+GF+P DTR ++ K
Sbjct: 79 VKIFYGSQTGTAKGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEAAIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWLWMLGAHRVMSRGEGDCDVVKSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQRGSEEREEGSHEQDELHHRDTEEEEPFESSSEEEFGGKDHQSLNSIVDVEDLGKIMDHVKKEKREKEQREEKSGLFRNMGRNEDGEIRAMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWRRFLDSLKALAVKQQRTVYRLTLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCGESSASSLTMAHVPWHEEVVQFVCELVDLIPDYEIACEHEHSNCLLIAHKKFKIGGEWWTWIDYNRFQELIQEYEDSSGSKTFSAKDYMARTPHWALFGANERGFDPKDTRHQRKNK 725
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. UniProt/SwissProt
Match: sp|Q9NV66|TYW1_HUMAN (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Homo sapiens OX=9606 GN=TYW1 PE=2 SV=2) HSP 1 Score: 672.159 bits (1733), Expect = 0.000e+0 Identity = 346/647 (53.48%), Postives = 434/647 (67.08%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTCT--SETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEI----TEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMMMR-------------------KRKDIVENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQ------TGKDFDATEYMAPTPSWAAYGSNEKGFNPSDTRFRKLRK 1975
+KI YGS TG +K A ++ + ++N+ YDP+++L +E K++ V V+TY +G P E A+WF WL +A DFR K +L+ +++AV GLG+S Y S+FN V K +D+ L R++ G D +VV SK+G ++ D++ W KF L E K S G C K + + +E+ E+ TEE E + +E ++ S N +VD+EDLG +M + E+ R M+TP LR +L KQGY+LIGSHSGVK+CRWTKSMLRGRGGCYKH FYGI SHRCME TPSLACANKCVFCWRHH+NPVGTEW+W MD PEMI+ EA+ H M+KQFKGVPGV+ ERF EG+ KHCALSLVGEPIMYP IN + LLH +IS+FLVTNAQFP I+ L PVTQLYVSVDA KD+LKKIDRPLFKDFW+RF+ SL A+ K+QRTVYRLTLVKAWNVDEL AYA LV LG P FIEVKGVTYCG+S AS LTMA+VPWHEEV+ FV +L D + YEIA EHEHSNC+L+A +KFK+ EW TWIDY +F EL+Q K F A +YMA TP WA +G++E+GF+P DTR ++ K
Sbjct: 79 VKIFYGSQTGTAKGFATVLAEAVTSLDLPVAIINLKEYDPDDHLIEEVTSKNVCVFLVATYTDGLPTESAEWFCKWLEEASIDFRFGKTYLKGMRYAVFGLGNSAYASHFNKVGKNVDKWLWMLGAHRVMSRGEGDCDVVKSKHGSIEADFRAWKTKFISQLQALQKGERKKSCGGHCKKGKCESHQHGSEEREEGSHEQDELHHRDTEEEEPFESSSEEEFGGEDHQSLNSIVDVEDLGKIMDHVKKEKREKEQQEEKSGLFRNMGRNEDGERRAMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDQPEMILKEAIENHQNMIKQFKGVPGVKAERFEEGMTVKHCALSLVGEPIMYPEINRFLKLLHQCKISSFLVTNAQFPAEIRNLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLKALAVKQQRTVYRLTLVKAWNVDELQAYAQLVSLGNPDFIEVKGVTYCGESSASSLTMAHVPWHEEVVQFVHELVDLIPEYEIACEHEHSNCLLIAHRKFKIGGEWWTWIDYNRFQELIQEYEDSGGSKTFSAKDYMARTPHWALFGASERGFDPKDTRHQRKNK 725
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. UniProt/SwissProt
Match: sp|Q8BJM7|TYW1_MOUSE (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1 OS=Mus musculus OX=10090 GN=Tyw1 PE=1 SV=1) HSP 1 Score: 671.389 bits (1731), Expect = 0.000e+0 Identity = 341/650 (52.46%), Postives = 433/650 (66.62%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL---------TCTSETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMMM-------------------RKRKDIVENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELV----QTG--KDFDATEYMAPTPSWAAYGSNEKGFNPSDTRFRKLRK 1975
+KI YGS TG +K A +K + ++N+ YDP+++L E K++ V+TY +G P E A+WF WL ++ +DFR K +L+ L++AV GLG S Y S+FN VS +D+ L +R+L G D N V SK+G ++ D+ W KF L C K + + E+ E E +S+DE+ T D + S +VD+EDLG +M ++ + E+ R M+TP LR +L KQGY+LIGSHSGVK+CRWTKSMLRGRGGCYKH FYGI SHRCMEATPSLACANKCVFCWRHH+NPVGTEW+W MD PE+I+ EA+ H M+KQFKGVPG++ ERF EG++ KHCALSLVGEPIMYP IN L+ LLH IS+FLVTNAQFP+ I++L PVTQLYVSVDA +D LKKIDRPLFKDFW+RF+ SL A+ K+QRTVYRLTLVK WNVDEL AYA LV LG P FIEVKGVTYCG+S AS LTMANVPWHEEV+ FV++L D L +YE+A EHEHSNC+L+ KKFK++ EW TWI+Y +F ELV ++G K F + +YMA TP WA +G+ E+GF+P DTR+++ K
Sbjct: 71 VKIFYGSQTGTAKGFAVVLAKAVTSLDLPVAIINLKEYDPDDSLIGEITSKTVCAFLVATYTDGCPTESAEWFCKWLEESANDFRFGKTYLKGLRYAVFGLGDSAYRSHFNKVSTNVDKWLWMLGAQRVLTRGEGDCNAVQSKHGSIEADFTAWKTKFISRLQALQRGEKKACGGNCKRGKCESAQHGPGEARPHPQGELHPGDAEEEEPCESSSEDELGTQDYQ------SLTSVVDVEDLGNIMNPVKREKREKSHQDGKAAMQRNPEKTEDGEGRAMITPALREALTKQGYQLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMEATPSLACANKCVFCWRHHTNPVGTEWRWKMDQPELILKEAIENHQNMIKQFKGVPGLKAERFEEGMEVKHCALSLVGEPIMYPEINRLLKLLHQHGISSFLVTNAQFPEEIRKLTPVTQLYVSVDASTRDGLKKIDRPLFKDFWQRFLDSLKALSAKQQRTVYRLTLVKCWNVDELQAYAELVSLGNPDFIEVKGVTYCGESAASSLTMANVPWHEEVVRFVRELVDLLPDYEVACEHEHSNCLLIGHKKFKIDGEWWTWINYSRFQELVLQYEESGGSKTFSSRDYMARTPQWALFGARERGFDPKDTRYQRKNK 714
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. TrEMBL
Match: A0A1S3KHL1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Lingula unguis OX=7574 GN=LOC106181776 PE=4 SV=1) HSP 1 Score: 731.095 bits (1886), Expect = 0.000e+0 Identity = 351/657 (53.42%), Postives = 465/657 (70.78%), Query Frame = 2
Query: 110 ETNINEKNT------IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTCTSETK--------NSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMMM---------RKRKDIVENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQT------GKDFDATEYMAPTPSWAAYGSNEKGFNPSDTRF-RKLRKHI 1981
E N+N+ + +KI +G+ TG +K A+ KE ++ +++++ TYDPE+N+ E K++ V +STY G+PP+DA WFY WL +A DFR+ K L+++++ VVGLG+S Y+ +N V++ ID F LS R+L IG+ DENV +SKNGG++ D++ W +FW + + + K ++GD + ++S + + ++ + N +VD+ED+G M+ RK +D ++K PREM+TP+LRTSL+KQGY+LIGSHSGVK+CRWTKSMLRGRGGCYKH FYGI SHRCME TPSLACANKCVFCWRHH+NPVGTEW+W MD PE+I++ A+ H M+KQFKGVPGV+ +RF EGL PKHCALSLVGEPIMYP IN +LHS+ IS+FLVTNAQFPDAI+ L PVTQLYVSVDA K++LKKIDRPLF+D+WERF+ SL A+ +K QRTVYRLTLVK +N ++++ YA+LV +G+P FIE+KGVTYCG+SKAS+LTM NVPWHEEV+ FVQ + DRL +Y IA+EHEHSNCVL+A KKF+VN +W TWIDY++FHELVQ K F A +YMA TP WA +G++E+GF+P +TRF RK K I
Sbjct: 58 ERNLNKAKSSKKETCLKIFFGTQTGTAKTFADIIKKEASEHSTKCEVIDLKTYDPEDNITDETDGKNICVFIISTYTEGSPPDDAAWFYKWLEEAASDFRIQKTMLQDMRYCVVGLGNSLYKERYNKVARNIDHWFHKLSANRVLGIGLLDENVASSKNGGIEADFEFWKNRFWSMVLGSPQNKKDVKPNCGTADGDTKEMYETSSEEDTEDEEEEGDGDGEKVQKANG--------------------VVDVEDIGKMVNNIKKAKAKRKVEDDNKSKEPREMITPLLRTSLQKQGYRLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDDPELILEGALQNHCNMIKQFKGVPGVKSDRFTEGLTPKHCALSLVGEPIMYPEINKFAEMLHSKGISSFLVTNAQFPDAIRNLTPVTQLYVSVDASTKESLKKIDRPLFRDYWERFLDSLKALSEKGQRTVYRLTLVKNYNTEDIENYASLVSIGKPDFIEIKGVTYCGESKASRLTMDNVPWHEEVIKFVQDVVDRLPDYAIASEHEHSNCVLVAHKKFQVNGDWWTWIDYDRFHELVQMYKASEGQKTFSAADYMAQTPHWAVFGADERGFDPGETRFMRKKTKDI 694
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. TrEMBL
Match: A0A1S3H4E9 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase OS=Lingula unguis OX=7574 GN=LOC106151340 PE=4 SV=1) HSP 1 Score: 729.554 bits (1882), Expect = 0.000e+0 Identity = 347/642 (54.05%), Postives = 459/642 (71.50%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTCTSETK--------NSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMMM---------RKRKDIVENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQT------GKDFDATEYMAPTPSWAAYGSNEKGFNPSDTRF-RKLRKHI 1981
+KI +G+ TG +K A+ KE ++ +++++ TYDPE+N+ E K++ V +STY G+PP+DA WFY WL +A DFR+ K L+++++ VVGLG+S Y+ +N V++ ID F LS R+L IG+ DENV +SKNGG++ D++ W +FW + + + K ++GD + ++S + + ++ + N +VD+ED+G M+ RK +D ++K PREM+TP+L+TSL+KQGY+LIGSHSGVK+CRWTKSMLRGRGGCYKH FYGI SHRCME TPSLACANKCVFCWRHH+NPVGTEW+W MD PE+I++ A+ H M+KQFKGVPGV+ +RF EGL PKHCALSLVGEPIMYP IN +LHS+ IS+FLVTNAQFPDAI+ L PVTQLYVSVDA K++LKKIDRPLF+D+WERF+ SL A+ +K QRTVYRLTLVK +N ++++ YA+LV +G+P FIE+KGVTYCG+SKAS+LTM NVPWHEEV+ FVQ + DRL +Y IA+EHEHSNCVL+A KKF+VN +W TWIDY++FHELVQ K F A +YMA TP WA +G++E+GF+P +TRF RK K I
Sbjct: 73 VKIFFGTQTGTAKTFADIIKKEASEHSTKCEVIDLKTYDPEDNITDETDGKNICVFIISTYTEGSPPDDAAWFYKWLEEAASDFRIQKTMLQDMRYCVVGLGNSLYKERYNKVARNIDHWFHKLSANRVLGIGLLDENVASSKNGGIEADFEFWKNRFWSMVLGSPQNKKDVKPNCGTADGDTKEMYETSSEEDTEDEEEEGDGDGEKVQKANG--------------------VVDVEDIGKMVNNIKKAKAKRKVEDDNKSKEPREMITPLLKTSLQKQGYRLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDDPELILEGALQNHCNMIKQFKGVPGVKSDRFTEGLTPKHCALSLVGEPIMYPEINKFAEMLHSKGISSFLVTNAQFPDAIRNLTPVTQLYVSVDASTKESLKKIDRPLFRDYWERFLDSLKALSEKGQRTVYRLTLVKNYNTEDIENYASLVSIGKPDFIEIKGVTYCGESKASRLTMDNVPWHEEVIKFVQDVVDRLPDYAIASEHEHSNCVLVANKKFQVNGDWWTWIDYDRFHELVQMYKASEGQKTFSAADYMAQTPHWAVFGADERGFDPGETRFMRKKTKDI 694
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. TrEMBL
Match: A0A2T7PHN4 (Flavodoxin-like domain-containing protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_08376 PE=4 SV=1) HSP 1 Score: 726.472 bits (1874), Expect = 0.000e+0 Identity = 361/650 (55.54%), Postives = 455/650 (70.00%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKEK---SLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTCT---SETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEEKNMD-------------SGNELVDMEDLGMMM--------RKRKDIVE-NKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQT------GKDFDATEYMAPTPSWAAYGSNEKGFNPSDTRF-RKLRKHI 1981
+KI YG+ TGKSK A++ + E Y+ ++++M TYDPEENL +E S+ V VSTY G PPED +WF+ WL D DFRV K L + +AV GLG+S Y+ NFN+V + +D LS L+ + DEN S+NGG++ D+ EW K WK L + K+ GD C K +C + + T E++ +SDEE + + + LVD+EDLG +M ++ K++ + N PREM+TP+LR SLEKQGY+LIGSHSGVK+CRWTKSMLRGRGGCYKH FYGI SHRC+E TPSLACANKCVFCWRHH+NPVGTEWKW MD P+ I D A+ H+ +VKQFKGVPGV+ ER E L+P+HCALSLVGEPIMYP IN V +LH + IS+FLVTNAQFPDAI+ L+PVTQLYVSVDA K++LKKIDRPLF+D+WERF+ SL A+ +K+QRTVYRLTLVK WN +ELD YA+L+ +G P FIEVKGVTYCG+SKAS LTM NVPWHEEV+ FV+QLA+RL YE+A+EHEHSNC+LLA K+FKV+ EW TWI+Y KFH L+Q F +T+YMA TP+WA YG+ E+GF+P +TRF RK +K I
Sbjct: 127 LKIFYGTQTGKSKAFAQRLAAEVKAYGYNVELVDMKTYDPEENLAEEAVDGSVCVFLVSTYTGGKPPEDTEWFFKWLEDTALDFRVQKTLLSGMCYAVFGLGNSLYKENFNVVGRNLDSWLQELSAIPLVSMMTGDENTAESRNGGIEEDFLEWKKALWKMLKPALDGRKVKHCKGDTCSKKCENCKSGKLGEAG-------DATHEHTKGGC-SSDEEIQFETSSEEEEEERETEGMTNSGLVDVEDLGKLMELAKTARTKEEKEMTQDNGEPREMITPLLRESLEKQGYRLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCVETTPSLACANKCVFCWRHHTNPVGTEWKWKMDDPKTIFDGALKNHVNLVKQFKGVPGVKPERLAEALEPQHCALSLVGEPIMYPEINAFVTMLHEKGISSFLVTNAQFPDAIRALLPVTQLYVSVDAATKESLKKIDRPLFRDYWERFLDSLKALSEKQQRTVYRLTLVKGWNTEELDNYASLISIGNPDFIEVKGVTYCGESKASNLTMVNVPWHEEVVDFVKQLAERLPEYELASEHEHSNCLLLANKRFKVDGEWWTWINYPKFHALIQQYTSSNGAATFASTDYMAKTPAWAVYGATEQGFDPVETRFYRKKQKDI 768
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. TrEMBL
Match: A0A0L8FXJ3 (Flavodoxin-like domain-containing protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22005636mg PE=4 SV=1) HSP 1 Score: 726.087 bits (1873), Expect = 0.000e+0 Identity = 369/647 (57.03%), Postives = 454/647 (70.17%), Query Frame = 2
Query: 128 KNTIKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKEKS---LLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKT----LTCTSETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMMMRKRKDI----------------VENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQTGKD-----FDATEYMAPTPSWAAYGSNEKGFNPSDTR-FRKLRKHI 1981
K ++ ILYGS TG SK LAE ++ +++N+ DPE+++ + KS + VI +STY G+PP+DA WFY WL + DFRV KNFLE + +AV G+S Y+ NF++V K ID+ L R+L +GI DE+ S +GGL D+ W++KFWK L + SN + + CC +NS+S+ + EE D + + S +G LVD+EDLG +M+ K +N+ P+ M+T + + SLEKQGY LIG+HSGVK+CRWTKSMLRGRGGCYKH FYGI SHRCME TPSLACANKCVFCWRHH+NPVGTEW+W+MD PE I + A+A H +M+KQF+GVPGV ERF EG+ PKHCALSLVGEPIMYP IN + LLH++ ISTFLVTNAQFPDAI L+PVTQLYVSVDA K++LKKIDRPLF+DFW+RF+ SL A+ +KKQRTVYRLTLVK WN DE++AYA+LVD G+P FIEVKGVTYCG+SKAS LTM NVPWHEEV FVQQLAD L +YEIA EHEHSNC+L+A KKFKVN EW TWIDYEKFHELVQ K+ F A +YMA TP WA YG+ E GF+PSD R FRK +K I
Sbjct: 66 KRSLHILYGSQTGHSKTLAESLAQNAEQQGVQVEVLNIKDCDPEDHIKQFKSPDRIYVIVISTYVQGSPPDDAVWFYKWLKETAVDFRVQKNFLEGMIYAVFSHGNSEYKENFSVVGKNIDDWLHQLEANRILPVGIGDEDTTQSSHGGLTEDFNAWNRKFWKKSIPLLKGGTLPVESNKCCKETCEKKCCDKDNSSSNQEVLMETSSEEEEEDGDNVESSGSLPVTQSNG--LVDLEDLGKVMKNVKKTKTKLKLNGYIVSTNKQTKNEEPKPMITEIFKKSLEKQGYSLIGTHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWLMDDPEYIFNGAIANHTKMIKQFRGVPGVLPERFNEGMSPKHCALSLVGEPIMYPEINKFIDLLHNKGISTFLVTNAQFPDAIHNLIPVTQLYVSVDASNKESLKKIDRPLFRDFWQRFLDSLQALAEKKQRTVYRLTLVKGWNTDEIEAYADLVDYGKPDFIEVKGVTYCGESKASTLTMKNVPWHEEVCGFVQQLADLLPDYEIACEHEHSNCLLVANKKFKVNGEWWTWIDYEKFHELVQNRKNNSEMIFTAEDYMAKTPDWAVYGAKEHGFDPSDKRFFRKKQKDI 710
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. TrEMBL
Match: V4CQI9 (Flavodoxin-like domain-containing protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_135774 PE=4 SV=1) HSP 1 Score: 717.613 bits (1851), Expect = 0.000e+0 Identity = 358/646 (55.42%), Postives = 437/646 (67.65%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENL---NKEKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTCT---SETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMMMRK------------------RKDIVENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQ------TGKDFDATEYMAPTPSWAAYGSNEKGFNPSDTR-FRKLRKHI 1981
+K+ YG+ TG SK AE + E Y +++++ TYDPE+NL N ++ V +STY +G PPE A WFY WL D DDFRV K L+ L + +VGLG+S Y+ N+ V+K +D++ LS + E CDEN VNS NGGL+ D+ W + FWK + K+ S + + C +++ EEN + S + K + + +VD+EDLG +M+K KDI E REM+TP+LR SLEKQGYKLIGSHSGVK+CRWTKSMLRGRGGCYKH FYGI SHRCME TPSLACANKCVFCWRHH+NPVGTEWKW MD E ++ A+ H +M+ +FKGVPGV ERF EG+ P+HCALSLVGEPIMYP IN + LLH + ISTFLVTNAQFPDAI++L P+TQLYVSVDA K+ LKKIDRPLFKDFWERF+ L + +K QRTVYRLTLVKAWN +E++ YA LVDLG+P FIEVKGVTYCGDSKAS LTM NVPWHEEV+ FV L DRL +YEIA+EHEHSNC+LLA KK KV EW TWIDY KFHEL+ K F +YMA TP WA YG+ E+GF+P +TR RK +K I
Sbjct: 1 MKVFYGTQTGNSKKFAEILAYEYELRGYGTEVVDLKTYDPEDNLVEENANGNICVFIISTYTDGQPPEGASWFYKWLTDTSDDFRVQKTLLKGLYYTIVGLGNSVYKDNYCTVAKKLDKSLHDLSATKFSETIFCDENTVNSLNGGLESDFAAWRENFWKKAQPALNGRKVKDCGKKCDGKCACSKPGVDGQEAGPCSSDEGSGEEENQFNSKNKSSPNKVKPANKTSGVVDLEDLGKIMKKAKICIILYKRKKLFFFQQNKDIEETGEVREMITPLLRQSLEKQGYKLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWKWKMDDAETLIAGALKNHTKMINEFKGVPGVIPERFQEGMNPQHCALSLVGEPIMYPEINKFIDLLHEKHISTFLVTNAQFPDAIRELTPITQLYVSVDANTKETLKKIDRPLFKDFWERFIECLKRLGEKGQRTVYRLTLVKAWNTEEIENYAKLVDLGRPDFIEVKGVTYCGDSKASSLTMENVPWHEEVIKFVTDLTDRLPDYEIASEHEHSNCLLLAHKKLKVKGEWWTWIDYPKFHELISEYTLSNGKKTFTTEDYMAKTPPWAVYGATERGFDPLETRHHRKKQKDI 646
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Cavefish
Match: tyw1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Source:NCBI gene;Acc:103036157]) HSP 1 Score: 695.271 bits (1793), Expect = 0.000e+0 Identity = 350/646 (54.18%), Postives = 436/646 (67.49%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTCTS--ETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMMM---RKRKDIVENKT-------------------PREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQTGKD------FDATEYMAPTPSWAAYGSNEKGFNPSDTRFRKLRK 1975
++I YGS TG +K A + + + + ++ +M YDP++ L+ E K + V V+TY +G P E+A+WF WL +A DFR K +L+ +++AV GLG+S Y ++N V K +D+ +LS R+L G D NVV S+NG + D+Q W KF L + E K +G + + + + E E + E + + ++DMEDLG +M +K K E ++ REM+TP LR +L KQGYKLIGSHSGVK+CRWTKSMLRGRGGCYKH FYGI SHRCME TPSLACANKCVFCWRHH+NPVGTEW+W MDP E I+ EAV H M++QF+GVPGV+ ERF EGL KHCALSLVGEPIMYP IN + LLH R IS+FLVTNAQFP+ I+ LVPVTQLYVSVDA KD+LKKIDRPLFKDFW+RF+ SL A+ +K+QRTVYRLTLVKAWNVDEL AY+ L+ LGQP FIEVKGVTYCG+S AS LTMANVPWHEEV+ FVQQL + L +YEIA EHEHSNC+LLA KFKV EW TWIDYE+F EL+Q +D F A +Y+A TPSWA +G E+GF+PSDTRF++ K
Sbjct: 79 VRIFYGSQTGTAKGFARELADDARAAGFHSEVFDMKDYDPDDRLSDECTSKMICVFLVATYTDGKPTENAEWFCKWLEEASTDFRYGKTYLKGMRYAVFGLGNSEYVGHYNTVGKNVDKWLWALSASRVLSRGEGDCNVVKSRNGSVQADFQAWKAKFLTRLQALARGEKKACSGKCKKSSCKNKKEHQQEAVDEHQ----EKHSSEELFESSSDSESAGEEEKAHGSVIDMEDLGNVMNHMKKAKKQSEEESQRGKLSKLNGVMKTEGEEEAREMITPALREALTKQGYKLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDPAEKILQEAVENHQNMIRQFRGVPGVRPERFEEGLTVKHCALSLVGEPIMYPEINAFLRLLHQRHISSFLVTNAQFPEEIRSLVPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLRALGEKQQRTVYRLTLVKAWNVDELKAYSELIALGQPDFIEVKGVTYCGESSASSLTMANVPWHEEVIHFVQQLENLLPDYEIACEHEHSNCLLLAHHKFKVEGEWWTWIDYERFQELIQRYEDSGGAETFSALDYVAKTPSWALFGGRERGFDPSDTRFQRKNK 720
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Cavefish
Match: tyw1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Source:NCBI gene;Acc:103036157]) HSP 1 Score: 681.404 bits (1757), Expect = 0.000e+0 Identity = 344/634 (54.26%), Postives = 427/634 (67.35%), Query Frame = 2
Query: 173 KILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTCTS--ETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMMM---RKRKDIVENKT-------------------PREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQTGKD------FDATEYMAPTPSWAAYGSNEKGFNPSDTRFRKLRK 1975
K A + + + + ++ +M YDP++ L+ E K + V V+TY +G P E+A+WF WL +A DFR K +L+ +++AV GLG+S Y ++N V K +D+ +LS R+L G D NVV S+NG + D+Q W KF L + E K +G + + + + E E + E + + ++DMEDLG +M +K K E ++ REM+TP LR +L KQGYKLIGSHSGVK+CRWTKSMLRGRGGCYKH FYGI SHRCME TPSLACANKCVFCWRHH+NPVGTEW+W MDP E I+ EAV H M++QF+GVPGV+ ERF EGL KHCALSLVGEPIMYP IN + LLH R IS+FLVTNAQFP+ I+ LVPVTQLYVSVDA KD+LKKIDRPLFKDFW+RF+ SL A+ +K+QRTVYRLTLVKAWNVDEL AY+ L+ LGQP FIEVKGVTYCG+S AS LTMANVPWHEEV+ FVQQL + L +YEIA EHEHSNC+LLA KFKV EW TWIDYE+F EL+Q +D F A +Y+A TPSWA +G E+GF+PSDTRF++ K
Sbjct: 46 KGFARELADDARAAGFHSEVFDMKDYDPDDRLSDECTSKMICVFLVATYTDGKPTENAEWFCKWLEEASTDFRYGKTYLKGMRYAVFGLGNSEYVGHYNTVGKNVDKWLWALSASRVLSRGEGDCNVVKSRNGSVQADFQAWKAKFLTRLQALARGEKKACSGKCKKSSCKNKKEHQQEAVDEHQ----EKHSSEELFESSSDSESAGEEEKAHGSVIDMEDLGNVMNHMKKAKKQSEEESQRGKLSKLNGVMKTEGEEEAREMITPALREALTKQGYKLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDPAEKILQEAVENHQNMIRQFRGVPGVRPERFEEGLTVKHCALSLVGEPIMYPEINAFLRLLHQRHISSFLVTNAQFPEEIRSLVPVTQLYVSVDASTKDSLKKIDRPLFKDFWQRFLDSLRALGEKQQRTVYRLTLVKAWNVDELKAYSELIALGQPDFIEVKGVTYCGESSASSLTMANVPWHEEVIHFVQQLENLLPDYEIACEHEHSNCLLLAHHKFKVEGEWWTWIDYERFQELIQRYEDSGGAETFSALDYVAKTPSWALFGGRERGFDPSDTRFQRKNK 675
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Cavefish
Match: porb (NADPH--cytochrome P450 reductase-like [Source:NCBI gene;Acc:103023972]) HSP 1 Score: 75.485 bits (184), Expect = 9.172e-14 Identity = 51/167 (30.54%), Postives = 85/167 (50.90%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEE----NLNKEK----SLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL 613
I + YGS TG + A + SK+ Y K M DPEE L++ K SL + ++TY G P ++A+ FY+WL ++ D L+ + F V LG+ YE ++N + K +D+ L KR+ ++G+ D+ +G L+ D+ W ++FW +
Sbjct: 79 IVVFYGSQTGTGEEFANRLSKDA--QRYGMKGM---AADPEEYDMSELSRLKEIDNSLAIFCMATYGEGDPTDNAQDFYDWLQESDAD-------LDGVNFTVFALGNKTYE-HYNAMGKYVDKRLSELGAKRIFDLGMGDD------DGNLEEDFVSWREQFWPAV 226
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Cavefish
Match: pora (NADPH--cytochrome P450 reductase [Source:NCBI gene;Acc:103023494]) HSP 1 Score: 69.3218 bits (168), Expect = 8.321e-12 Identity = 49/170 (28.82%), Postives = 81/170 (47.65%), Query Frame = 2
Query: 128 KNTIKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE--------KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL 613
+N I + YGS TG ++ A + K+ Y K M DPEE E SL V ++TY G P ++A+ FY+W+ +V LE + F+V GLG+ Y+ ++N + K D L ++ ++G+ D+ +G L+ D+ W +FW +
Sbjct: 35 RNVI-VFYGSQTGTAEEFATRLVKDA--QRYGIKGM---VADPEEYDMSELPRLTEVVNSLAVFCMATYGEGEPTDNAQGFYDWM-------QVTDVSLEGVHFSVFGLGNKTYD-HYNAMGKYTDRRLAELGATQIFDLGLGDD------DGNLEEDFISWKAQFWPAV 184
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Cavefish
Match: mtrr (5-methyltetrahydrofolate-homocysteine methyltransferase reductase [Source:NCBI gene;Acc:103046907]) HSP 1 Score: 61.6178 bits (148), Expect = 1.882e-9 Identity = 43/157 (27.39%), Postives = 76/157 (48.41%), Query Frame = 2
Query: 143 ILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKEKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL 613
ILYGS G+++ +AE+ S++ + + K+ ++ D + NL++E +V VST +G PP+ A F + + + + +L +A++GLG + Y +NF K ID+ L K G D+ GL+ W + W+ L
Sbjct: 11 ILYGSQRGQAQSIAEEISEQATEHGFLAKISCLSKGD-KYNLDREHGPVVFVVSTTGDGEPPDTALKFVKKIKNKS----LPGDHYSHLHYALLGLGDTNY-ANFCNCGKTIDKRLQELGAKHFYATGHADDGT------GLEVVVDPWIEGLWEAL 155
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008170.1 (pep scaffold:Pmarinus_7.0:GL479645:6967:26481:1 gene:ENSPMAG00000007386.1 transcript:ENSPMAT00000008170.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 115.161 bits (287), Expect = 9.548e-29 Identity = 87/302 (28.81%), Postives = 139/302 (46.03%), Query Frame = 2
Query: 170 SKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTCTSETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEEK--------NMDSGNELVDMEDLGMMM----------------RKRKDIVENKT-------PREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTK 973
+K+LAE+ C+ + + ++ ++DPE++L +E I Y + + + WL +A DFR K L +++AV GLG S Y+ ++N V K +D+ L +R+L G D NVV S++G D D++ W + D + S + + E+ E+ ++ TS EE +LVD+EDLG ++ ++R+ + K PREM+TP LR +L KQGY+LIG+HSGVK+CRWTK
Sbjct: 2 AKLLAEELG--CL--GFTSSVSDLRSFDPEDSLLEEVPQPRSQYIRSPYYPHLRVGKHNECHCKWLEEAASDFRYGKTHLRGVRYAVFGLGDSVYQEHYNTVGKNMDKWLWQLGARRVLPRGDGDFNVVRSRHGSADADFEAWKGHLVRLRALA--------DPGTAAGAGVGGQQRQRSDSEGSCRDELEEDRTNPNENTSKASEEFETTSEEEGGTADAADLVDVEDLGRIVTEARRSQKSHVDEDDVKRRRRVAAGKGRATSTEEPREMITPALREALTKQGYRLIGTHSGVKLCRWTK 291
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008175.1 (pep scaffold:Pmarinus_7.0:GL479645:6967:26481:1 gene:ENSPMAG00000007386.1 transcript:ENSPMAT00000008175.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 73.9442 bits (180), Expect = 9.969e-15 Identity = 44/143 (30.77%), Postives = 75/143 (52.45%), Query Frame = 2
Query: 170 SKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEW 589
+K+LAE+ C+ + + ++ ++DPE++L +E I Y + + + WL +A DFR K L +++AV GLG S Y+ ++N V K +D+ L +R+L G D NVV S++G D D++ W
Sbjct: 2 AKLLAEELG--CL--GFTSSVSDLRSFDPEDSLLEEVPQPRSQYIRSPYYPHLRVGKHNECHCKWLEEAASDFRYGKTHLRGVRYAVFGLGDSVYQEHYNTVGKNMDKWLWQLGARRVLPRGDGDFNVVRSRHGSADADFEAW 140 HSP 2 Score: 69.3218 bits (168), Expect = 3.163e-13 Identity = 35/79 (44.30%), Postives = 47/79 (59.49%), Query Frame = 2
Query: 803 ELVDMEDLG----------------MMMRKRK------DIVENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTK 973
+LVD+EDLG ++ R+R+ + PREM+TP LR +L KQGY+LIG+HSGVK+CRWTK
Sbjct: 211 DLVDVEDLGRIVTEARRSQNQEDESLLTRRRRVAAGKGRATSTEEPREMITPALREALTKQGYRLIGTHSGVKLCRWTK 289
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Sea Lamprey
Match: porb (P450 (cytochrome) oxidoreductase b [Source:ZFIN;Acc:ZDB-GENE-030131-5767]) HSP 1 Score: 57.3806 bits (137), Expect = 8.170e-9 Identity = 34/113 (30.09%), Postives = 60/113 (53.10%), Query Frame = 2
Query: 275 EKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL 613
E SL V ++TY G P ++A+ FY+ L + + L +++AV GLG+ YE +FN + K +D+ L +R+ E+G D++ L+ D+ W ++FW +
Sbjct: 135 ENSLAVFCMATYGEGDPTDNAQEFYDLLQEGSLE-------LAGVKYAVFGLGNKTYE-HFNAMGKYLDKRLEELGAERIFELGSGDDDAT------LEEDFITWKERFWPAV 233
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Yeast
Match: TYW1 (Iron-sulfer protein required for synthesis of Wybutosine modified tRNA; Wybutosine is a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron [Source:SGD;Acc:S000006128]) HSP 1 Score: 454.907 bits (1169), Expect = 1.472e-150 Identity = 266/636 (41.82%), Postives = 372/636 (58.49%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFS---KECIHNNYDFKMMNMNTY----DPEENLNKEKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENL-QFAVVGLGSS-YYESNFNIVSKAIDEAFGSLSGKRLLEIG-ICDENVVNSKNGGLDYDYQEWSKKFWKTLTCTSETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMMMRKRK----DIVENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVK--DKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCG--DSKASKLTMANVPWHEEVLSFVQQLADRLE----NYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQTGKDFDATEYMAPTPSWAAYGSNEKGFNPSDTR-FRKLRK 1975
I +LY SL G + A+ KE + K++N++ D N+ E +L V+ + +Y+ P + +F L + +DFRVD L L + V+GLG S + F +K D L G+R+ +G +C K GG EW+ +TL I +EN+D E ++EEE N +EL D+ED+G K D ++ ++ +P + +L KQGYK+IGSHSGVK+CRWTK+ LRG+G CYK + + IAS RCME TPSLAC++KCVFCWRH +NPV W+W +D PE I++ A+ H M+KQ +GVPGV ERF + + +HCALSLVGEPI+YP IN + LLH + I++FLV NAQ P+A++ +V VTQLYVS+DA K LKK+DRPL+KDFWER V L +K QRTV+RLTLVK +N+ ++ AYA+LV G P FIEVKG T+ G D + LTM N+P++EE + FV+ L+ +Y++AAEH HSNC+L+A KFK+N EW+T ID++KF L+ +GKDF +Y+ TP WA +G+ GF P +TR +RK +K
Sbjct: 205 IYVLYSSLQGAASKAAKSVYDKLKELDELTNEPKLLNLDDLSDFDDYFINVPVENALYVLVLPSYDIDCPLD---YFLQTLEENANDFRVDSFPLRKLVGYTVLGLGDSESWPEKFCYQAKRADHWISRLGGRRIFPLGKVC------MKTGG-SAKIDEWTSLLAETL------------------------------KDDEPIIYEYDENADSE----EDEEEGN--GSDELGDVEDIGGKGSNGKFSGADEIKQMVAKD--SPTYK-NLTKQGYKVIGSHSGVKICRWTKNELRGKGSCYKKSLFNIASSRCMELTPSLACSSKCVFCWRHGTNPVSKNWRWEVDEPEYILENALKGHYSMIKQMRGVPGVIAERFAKAFEVRHCALSLVGEPILYPHINKFIQLLHQKGITSFLVCNAQHPEALRNIVKVTQLYVSIDAPTKTELKKVDRPLYKDFWERMVECLEILKTVQNHQRTVFRLTLVKGFNMGDVSAYADLVQRGLPGFIEVKGATFSGSSDGNGNPLTMQNIPFYEECVKFVKAFTTELQRRGLHYDLAAEHAHSNCLLIADTKFKINGEWHTHIDFDKFFVLLNSGKDFTYMDYLEKTPEWALFGNG--GFAPGNTRVYRKDKK 789
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Yeast
Match: NCP1 (NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p [Source:SGD;Acc:S000001084]) HSP 1 Score: 64.3142 bits (155), Expect = 3.190e-11 Identity = 52/165 (31.52%), Postives = 83/165 (50.30%), Query Frame = 2
Query: 122 NEKNTIKILYGSLTGKSKILAEKFSKECIHN-NYDFKMMNMNTYDPEENLNKEKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL 613
N KN + +LY S TG ++ A+KFSKE + N + ++ YD E+LN ++ IF+STY G P+ A F +++ +A + L NL++ + GLG+S YE FN +K ++ + RL ++G D+ G D DY W + L
Sbjct: 57 NNKNYL-VLYASQTGTAEDYAKKFSKELVAKFNLNVMCADVENYD-FESLNDVPVIVSIFISTYGEGDFPDGAVNFEDFICNA------EAGALSNLRYNMFGLGNSTYEF-FNGAAKKAEKHLSAAGAIRLGKLGEADDGA-----GTTDEDYMAWKDSILEVL 207
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Yeast
Match: MET5 (Sulfite reductase beta subunit; involved in amino acid biosynthesis, transcription repressed by methionine [Source:SGD;Acc:S000003898]) HSP 1 Score: 50.8322 bits (120), Expect = 6.904e-7 Identity = 46/168 (27.38%), Postives = 74/168 (44.05%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKEKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYY------ESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTCT 622
+ + Y S G + LA++ + +++M+ EE +E +V ST G P+D K F+ L K+D +D L +L AV GLG S Y + FN S+ + + LS K L+ +G+ D+ + G Y EW K W+ L +
Sbjct: 682 LHVYYASDGGNAANLAKRLAARASARGLKATVLSMDDIILEELPGEEN--VVFITSTAGQGEFPQDGKSFWEAL---KNDTDLD---LASLNVAVFGLGDSEYWPRKEDKHYFNKPSQDLFKRLELLSAKALIPLGLGDDQDAD----GFQTAYSEWEPKLWEALGVS 837
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Nematostella
Match: EDO44602 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RV91]) HSP 1 Score: 703.745 bits (1815), Expect = 0.000e+0 Identity = 345/612 (56.37%), Postives = 438/612 (71.57%), Query Frame = 2
Query: 206 IHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTCTSETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMMMRKRK----------DIVENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQTGKD------FDATEYMAPTPSWAAYGSNEKGFNPSDTR-FRKLRKHI 1981
+ D N+ Y+ E+NL E KS V V TY +G PP A+WFY+WL D+ +D RV K+ L + +AV GLG+S YE FN+V+K ID+ SLS KR++ + + DEN VNS++GGL+ D+Q W K F+ + + + + C S E T + D E TSD + E + D+ + LVDMEDLG ++K K D +N +EM+TP++R SL KQGYK+IGSHSGVK+CRWTKSMLRGRGGCYKH FYGI SHRCME TPSLACANKCVFCWRHH+NPVGTEW+W MD P+MIV+ A+ H M+KQF+GVPGV+ ERF EG+K KHCALSLVGEPIMYP+IN L+ LLH IS+FLVTNAQFPDAI+ L+PVTQLYVSVDAG K++LKKIDRPLF+DFW RF+ SL A+ DK QRTVYRLTLVK+WNV+E+ +Y LV LG+P FIEVKGVTYCG+SKAS LTMAN+PWH+EV+ FV++L D+L +YE+A+EHEHSNCVLLA KKFKVN EW+TWIDY KFH+L++ ++ F + +YM P W+ +G +++GF+P +TR +RK RK I
Sbjct: 5 MQQGMDCSCENLQGYEAEDNLANEVCTKSTCVFIVPTYTDGKPPRGAEWFYSWLEDSTNDCRVSKSLLSQINYAVFGLGNSSYEGQFNLVAKNIDKWLSSLSAKRVMPVTLGDENTVNSRHGGLEADFQVWCKTFFSLVKSHTLCECGKQETCQETSKSQ----------------EETSQACDYES-TSDGDSE-DEDTADGLVDMEDLGPAVQKIKKAKKQREIEEDSHKNTVAKEMVTPVIRQSLTKQGYKIIGSHSGVKICRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWQMDDPQMIVEGAMQNHYNMIKQFRGVPGVKPERFEEGMKIKHCALSLVGEPIMYPKINELIKLLHGHGISSFLVTNAQFPDAIKDLLPVTQLYVSVDAGTKESLKKIDRPLFRDFWPRFLDSLKALADKGQRTVYRLTLVKSWNVEEIQSYVELVSLGKPDFIEVKGVTYCGESKASTLTMANIPWHQEVVGFVRKLEDQLPDYELASEHEHSNCVLLAHKKFKVNGEWHTWIDYTKFHQLIEKYEESNGEETFTSADYMERNPEWSVFGHSQRGFDPEETRWYRKNRKDI 598
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Nematostella
Match: EDO28992 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T4X1]) HSP 1 Score: 81.6481 bits (200), Expect = 2.066e-16 Identity = 51/167 (30.54%), Postives = 86/167 (51.50%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE--------KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL 613
+ I YGS TG ++ A + +K+ Y K M +DPEEN +E S + ++TY G P ++A+ FY WL + +DD L++L FAV GLG+ YE ++N + + +D+ L G R+ + G D+ +G ++ D+ W ++FW +
Sbjct: 4 LAIFYGSQTGTAEEFASRLAKDA--QRYGIKAM---VFDPEENDMEELTRMGEIDNSFAIFVMATYGEGDPTDNAQEFYEWLQNDRDD-------LDSLNFAVFGLGNKTYE-HYNSMGRYVDKRLEELGGNRIYQRGEGDD------DGNIEEDFVRWREEFWPAV 151
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Nematostella
Match: EDO39434 (NADPH--cytochrome P450 reductase [Source:UniProtKB/TrEMBL;Acc:A7SA55]) HSP 1 Score: 78.9518 bits (193), Expect = 2.466e-15 Identity = 51/167 (30.54%), Postives = 85/167 (50.90%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE--------KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL 613
+ I YGS TG ++ A + +K+ Y K M +DPEEN +E S + ++TY G P ++A+ FY WL + +DD L+ L FAV GLG+ YE ++N + + +D+ L G R+ + G D+ +G ++ D+ W ++FW +
Sbjct: 72 LAIFYGSQTGTAEEFASRLAKDA--QRYGIKAM---VFDPEENDMEELTRMGEIDNSFAIFVMATYGEGDPTDNAQEFYEWLQNDRDD-------LDCLNFAVFGLGNKTYE-HYNSMGRYVDKRLEELGGYRIYQRGEGDD------DGNIEEDFVRWREEFWPAV 219
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Nematostella
Match: EDO40246 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7T0]) HSP 1 Score: 63.929 bits (154), Expect = 1.169e-10 Identity = 46/161 (28.57%), Postives = 77/161 (47.83%), Query Frame = 2
Query: 131 NTIKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKEKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL 613
N+ ILY S TG++K +A++ ++ N + K+ ++ + + L KE S +V VST G PP+ F+ L + N L++LQ+ ++ LG S Y +NF + K +D L K+ + G D+ V GL+ + W W L
Sbjct: 5 NSFTILYASQTGQAKAIADEIHEKSASNGLNSKLFCLSLTEKKFMLEKE-SAVVFVVSTTGEGDPPDTMLKFFRRLKKKT----LPGNHLKDLQYGLLALGDSNY-TNFCVNGKNLDRRLNELGAKKFYDTGHADDAV------GLELVVEPWIDGLWGPL 153
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Nematostella
Match: EDO39440 (Nitric oxide synthase [Source:UniProtKB/TrEMBL;Acc:A7SA75]) HSP 1 Score: 56.9954 bits (136), Expect = 2.589e-8 Identity = 48/168 (28.57%), Postives = 82/168 (48.81%), Query Frame = 2
Query: 143 ILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKEKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLT---CTSETKN 637
ILY + TGKS+ A + +H +D K+M M+ Y E N++ L++I ST+ NG PPE+ + +D+ R+ +E+ F GLGS Y NF ++++D+ L +++ + G DE G + ++ W+K+ +K C E N
Sbjct: 485 ILYATETGKSESYARMLADLFLHA-FDPKVMRMDEYPHPEMENEQ--LILIVTSTFGNGDPPENGEVSTINPLDSASQQRI----IEDQMF---GLGSRAY-PNFCAFARSLDKIIQELGAEQIHKCGEGDE------LAGQEESFKTWAKQVFKAACDTFCVGEMVN 635
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Medaka
Match: tyw1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Source:NCBI gene;Acc:101161427]) HSP 1 Score: 705.286 bits (1819), Expect = 0.000e+0 Identity = 353/648 (54.48%), Postives = 444/648 (68.52%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTC--TSETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMMMRKR------------------------KDIVENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQTGKD------FDATEYMAPTPSWAAYGSNEKGFNPSDTRFRKLRK 1975
+KI YGS TG +K A+ S+E + ++++M YDP++ L E S+ V V+TY +G P E A+WF WL DA DFR + +L+ L++AV GLG+S Y ++N V K +D+ LS KRL+ G D NVV S++G + D+Q W KF L T E K +G C S K +S ++E+ E +SDE+E +VD+EDLG +M+ K I ++ REM+T LR +L KQGYKLIGSHSGVK+CRWTKSMLRGRGGCYKH FYGI SHRCME TPSLACANKCVFCWRHH+NPVGTEW+W MDP E I+ +A+ KH M++QF+GVPGV+ ER+ EGL KHCALSLVGEPIMYP IN + LLHSR+IS+FLVTNAQFP+ I+ L PVTQLYVSVDA KD+LKKIDRPLFKDFW RF+ SL A+ +K+QRTVYRLTLVKAWNV+E+ AYA L+ LGQP FIEVKGVTYCG+S AS LTMANVPWH+EV++FVQQLA L YE+A EHEHSNC+L+A +KFK++ +W TWIDYE+F +LVQ +D F A +YMA TPSWA +G+ E+GF+P+DTRF++ K
Sbjct: 94 VKIFYGSQTGTAKGFAKVLSEELEALGFPAEVIDMKDYDPDDRLADECVSGSVCVFLVATYTDGQPTESAEWFCKWLEDASTDFRYGRTYLKGLRYAVFGLGNSVYVGHYNTVCKNVDKWLWMLSAKRLMTRGEGDCNVVKSRHGSVQADFQAWKGKFLSRLQALATGEKKTCSGKNCKTGSSCKSKKEEEEASKETGSEVELVES-------SSDEDESGLTGEKRSVVDLEDLGNLMKNAKKAKGKNGPQRDGEVVKFSKLNGVKKIDDDGERREMITAALREALTKQGYKLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDPAEKILQDALDKHQDMIRQFRGVPGVKPERYEEGLAVKHCALSLVGEPIMYPEINAFIRLLHSRRISSFLVTNAQFPEEIRSLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWPRFLDSLKALGEKRQRTVYRLTLVKAWNVEEMQAYAELIALGQPDFIEVKGVTYCGESSASTLTMANVPWHQEVVAFVQQLAAMLPEYEVACEHEHSNCLLIAHEKFKLDGDWWTWIDYERFQDLVQAQQDSGGRQTFSALDYMAKTPSWALFGAREQGFDPADTRFQRRNK 734
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Medaka
Match: tyw1 (S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase [Source:NCBI gene;Acc:101161427]) HSP 1 Score: 691.034 bits (1782), Expect = 0.000e+0 Identity = 347/624 (55.61%), Postives = 436/624 (69.87%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKE---KSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTC--TSETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMMMRKRKDIVENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQTGKD------FDATEYMAPTPSWAAYGSNEKGFNPSDTRFRKLRK 1975
+KI YGS TG +K A+ S+E + ++++M YDP++ L E S+ V V+TY +G P E A+WF WL DA DFR + +L+ L++AV GLG+S Y ++N V K +D+ LS KRL+ G D NVV S++G + D+Q W KF L T E K +G C S K +S ++ EE +LT + + E+V L + K I ++ REM+T LR +L KQGYKLIGSHSGVK+CRWTKSMLRGRGGCYKH FYGI SHRCME TPSLACANKCVFCWRHH+NPVGTEW+W MDP E I+ +A+ KH M++QF+GVPGV+ ER+ EGL KHCALSLVGEPIMYP IN + LLHSR+IS+FLVTNAQFP+ I+ L PVTQLYVSVDA KD+LKKIDRPLFKDFW RF+ SL A+ +K+QRTVYRLTLVKAWNV+E+ AYA L+ LGQP FIEVKGVTYCG+S AS LTMANVPWH+EV++FVQQLA L YE+A EHEHSNC+L+A +KFK++ +W TWIDYE+F +LVQ +D F A +YMA TPSWA +G+ E+GF+P+DTRF++ K
Sbjct: 79 VKIFYGSQTGTAKGFAKVLSEELEALGFPAEVIDMKDYDPDDRLADECVSGSVCVFLVATYTDGQPTESAEWFCKWLEDASTDFRYGRTYLKGLRYAVFGLGNSVYVGHYNTVCKNVDKWLWMLSAKRLMTRGEGDCNVVKSRHGSVQADFQAWKGKFLSRLQALATGEKKTCSGKNCKTGSSCKSKKEEEEASKETGSEVRRREEGV---LLTVTKLRD------GEVVKFSKLNGV----KKIDDDGERREMITAALREALTKQGYKLIGSHSGVKLCRWTKSMLRGRGGCYKHTFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWRWKMDPAEKILQDALDKHQDMIRQFRGVPGVKPERYEEGLAVKHCALSLVGEPIMYPEINAFIRLLHSRRISSFLVTNAQFPEEIRSLEPVTQLYVSVDASTKDSLKKIDRPLFKDFWPRFLDSLKALGEKRQRTVYRLTLVKAWNVEEMQAYAELIALGQPDFIEVKGVTYCGESSASTLTMANVPWHQEVVAFVQQLAAMLPEYEVACEHEHSNCLLIAHEKFKLDGDWWTWIDYERFQDLVQAQQDSGGRQTFSALDYMAKTPSWALFGAREQGFDPADTRFQRRNK 689
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Medaka
Match: POR (NADPH--cytochrome P450 reductase [Source:NCBI gene;Acc:101166486]) HSP 1 Score: 71.2478 bits (173), Expect = 1.697e-12 Identity = 50/167 (29.94%), Postives = 85/167 (50.90%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEE-------NLNKEKSLLVIF-VSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL 613
I + YGS TG ++ A + SK+ Y K M DPEE +++ K+ L IF ++TY G P ++A+ FY+WL + D+ L L + V LG+ YE ++N + K +D+ L KR+ ++G+ D+ + L+ D+ W ++FW +
Sbjct: 74 IIVFYGSQTGTAEEFANRLSKDA--QRYGMKGM---AADPEEYTMGELARMSEIKNSLAIFCMATYGEGDPTDNAQDFYDWLQENDDE------DLSGLNYTVFALGNKTYE-HYNAMGKYVDKRLEELGAKRIFDLGLGDD------DSNLEEDFVSWREQFWPAV 222
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Medaka
Match: mtrr (5-methyltetrahydrofolate-homocysteine methyltransferase reductase [Source:NCBI gene;Acc:101167253]) HSP 1 Score: 59.3066 bits (142), Expect = 1.025e-8 Identity = 44/166 (26.51%), Postives = 79/166 (47.59%), Query Frame = 2
Query: 128 KNTIKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKEKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKN----FLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL 613
K+ +LYGS G+++ +AE ++E + ++ +N D + NL KE + +V VST +G PP++A F R++KN ++L + ++ LG + Y +NF K I+ +L ++ G D+ V GL+ W + WK +
Sbjct: 6 KSRFVVLYGSQKGQAQSIAEGIAEEAEDHGLVAELSCLNQ-DEKYNLEKEIAPVVFVVSTTGDGEPPDNALQFVK---------RINKNQSPDHYKHLSYTLLALGDTNY-ANFCNCGKTIERRLQALGARQFYPAGFADDGV------GLELVVDPWLEGLWKAV 154
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Medaka
Match: mtrr (5-methyltetrahydrofolate-homocysteine methyltransferase reductase [Source:NCBI gene;Acc:101167253]) HSP 1 Score: 58.9214 bits (141), Expect = 1.522e-8 Identity = 44/166 (26.51%), Postives = 79/166 (47.59%), Query Frame = 2
Query: 128 KNTIKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKEKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKN----FLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL 613
K+ +LYGS G+++ +AE ++E + ++ +N D + NL KE + +V VST +G PP++A F R++KN ++L + ++ LG + Y +NF K I+ +L ++ G D+ V GL+ W + WK +
Sbjct: 34 KSRFVVLYGSQKGQAQSIAEGIAEEAEDHGLVAELSCLNQ-DEKYNLEKEIAPVVFVVSTTGDGEPPDNALQFVK---------RINKNQSPDHYKHLSYTLLALGDTNY-ANFCNCGKTIERRLQALGARQFYPAGFADDGV------GLELVVDPWLEGLWKAV 182
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Planmine SMEST
Match: SMESG000054804.1 (SMESG000054804.1) HSP 1 Score: 1253.81 bits (3243), Expect = 0.000e+0 Identity = 625/628 (99.52%), Postives = 627/628 (99.84%), Query Frame = 2
Query: 98 KFKTETNINEKNTIKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKEKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTCTSETKNSNGDVXXXXXXXXXXXXXXXXXXXXXXXIEITEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMMMRKRKDIVENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEVKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQTGKDFDATEYMAPTPSWAAYGSNEKGFNPSDTRFRKLRKHI 1981
KFKTETNINEKNTIKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKEKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFN+VSKAIDEAF SLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTCTSETKNSNGDVCDNKKSSCCKTNNSNSSCCKNEKIEITEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMMMRKRKDIVENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIE+KGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQTGKDFDATEYMAPTPSWAAYGSNEKGFNPSDTRFRKLRKHI
Sbjct: 10 KFKTETNINEKNTIKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEENLNKEKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNVVSKAIDEAFWSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTLTCTSETKNSNGDVCDNKKSSCCKTNNSNSSCCKNEKIEITEENSDDEVLTSDEEEEKNMDSGNELVDMEDLGMMMRKRKDIVENKTPREMLTPMLRTSLEKQGYKLIGSHSGVKMCRWTKSMLRGRGGCYKHAFYGIASHRCMEATPSLACANKCVFCWRHHSNPVGTEWKWIMDPPEMIVDEAVAKHIQMVKQFKGVPGVQEERFLEGLKPKHCALSLVGEPIMYPRINTLVGLLHSRQISTFLVTNAQFPDAIQQLVPVTQLYVSVDAGEKDALKKIDRPLFKDFWERFVASLTAVKDKKQRTVYRLTLVKAWNVDELDAYANLVDLGQPHFIEIKGVTYCGDSKASKLTMANVPWHEEVLSFVQQLADRLENYEIAAEHEHSNCVLLAQKKFKVNDEWNTWIDYEKFHELVQTGKDFDATEYMAPTPSWAAYGSNEKGFNPSDTRFRKLRKHI 637
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Planmine SMEST
Match: SMESG000022047.1 (SMESG000022047.1) HSP 1 Score: 72.0182 bits (175), Expect = 7.671e-13 Identity = 51/167 (30.54%), Postives = 81/167 (48.50%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEKFSKECIHNNYDFKMMNMNTYDPEE-------NLNK-EKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL 613
+ + YGS TG ++ A + +KE + Y K M DPEE NL E SL + ++TY G P ++A+ FYNWL +A + + L++ V GLG+ YE NFN + K D L R+ E+GI D+ + ++ + W + W ++
Sbjct: 92 VVVFYGSQTGTAEDFANRLAKEAVR--YGLKGM---AADPEECDMEELLNLKSIENSLAIFCMATYGEGDPTDNAQEFYNWLQNATVN-------MNGLRYTVFGLGNKTYE-NFNAMGKYFDRRLSELGATRIHELGIGDD------DSNIEDHFITWKEDMWTSV 239
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Planmine SMEST
Match: SMESG000056879.1 (SMESG000056879.1) HSP 1 Score: 60.077 bits (144), Expect = 4.211e-9 Identity = 49/162 (30.25%), Postives = 75/162 (46.30%), Query Frame = 2
Query: 137 IKILYGSLTGKSKILAEK---FSKECIHNNYDFKMMNMNTYDPEENLNKEKSLLVIFVSTYENGTPPEDAKWFYNWLIDAKDDFRVDKNFLENLQFAVVGLGSSYYESNFNIVSKAIDEAFGSLSGKRLLEIGICDENVVNSKNGGLDYDYQEWSKKFWKTL 613
+KI YGS TG ++ +AEK FS+ K+ MN D + + + + ST G PP++ K F W K F + L L FAV GLG S Y + +N ++K + L ++ IG+ D+ + G+D ++ W K WK L
Sbjct: 4 LKIFYGSQTGNAQDVAEKVYRFSQNLPPKYASVKIGEMN--DVAFDSLSQNDIFIFICSTTGQGDPPDNMKHF--WQCVMKRSFNPN---LSCLDFAVFGLGDSKY-NKYNFIAKKLYRRLIQLKANSMINIGLADD----QHSLGIDGEFSLWIKLLWKVL 153 The following BLAST results are available for this feature:
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 3
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 3
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30024454 ID=SMED30024454|Name=S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase|organism=Schmidtea mediterranea sexual|type=transcript|length=2024bpback to top protein sequence of SMED30024454-orf-1 >SMED30024454-orf-1 ID=SMED30024454-orf-1|Name=SMED30024454-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=652bp MFEVLALSLILPIILYYFIIHFLKFKTETNINEKNTIKILYGSLTGKSKIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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