SMED30024436

Overview
NameSMED30024436
Smed IDSMED30024436
Length (bp)1028
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30024436 (SMED30024436) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30024436 (SMED30024436) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30024436 (SMED30024436) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30024436 (SMED30024436) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30024436

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30024436SMESG000043617.1 dd_Smed_v4_8060_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30024436SMESG000043617.1 dd_Smed_v4_8060_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30024436SMESG000043617.1 dd_Smed_v4_8060_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30024436SMESG000043617.1 dd_8060dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
Note: Hover over icons to view figure legend
Alignments
SMED30024436 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.009577:2251..3904 +5010
Homology
[Close]
BLAST of SMED30024436 vs. TrEMBL
Match: A0A151WM12 (Tetraspanin OS=Trachymyrmex zeteki OX=64791 GN=ALC60_11912 PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 1.793e-7
Identity = 43/145 (29.66%), Postives = 70/145 (48.28%), Query Frame = 2 
Query:  212 LVIIVGIFMIILAIYSLFLVSKNHICQL---FISVIFYFFMAITLGAMLFHTWYDKQIYTKNRVV-TAIRSYDWKSFNSHHLDRLQNTLSCCGYESAADYHQSNNLFLKDNVPLSCCFD-NSKSIIKCNPNVTKINIDNAYPQGC 631
            ++++ GI +  +A +     S+   C L   FI V F  F+ + +G +L + + +K + T +R + +++R YD +       D  Q+TL CCG  +  D+          NVP SCC +        CN N   IN  NAY +GC
Sbjct:   62 VLLVGGIIVAFIAFFGCVGASREVKCMLLTYFIIVFFLLFVTMLIGGVLGYIFREKLLSTVDREMRSSLRLYDHRKSIRDAWDTTQSTLHCCGVNNWRDWGS-----FGLNVPDSCCREITPGQRFNCNQNPESINPSNAYVEGC 201
[Close]
BLAST of SMED30024436 vs. TrEMBL
Match: A0A026W8X0 (Tetraspanin OS=Ooceraea biroi OX=2015173 GN=DMN91_011518 PE=3 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 2.790e-7
Identity = 42/144 (29.17%), Postives = 72/144 (50.00%), Query Frame = 2 
Query:  212 LVIIVGIFMIILAIYSLFLVSKNHICQL--FISVIFYFFMAITLGAMLFHTWYDKQIYTKNRVV-TAIRSYDWKSFNSHHLDRLQNTLSCCGYESAADYHQSNNLFLKDNVPLSCCFD-NSKSIIKCNPNVTKINIDNAYPQGC 631
            ++++ GI + ++A +     S+   C L  +  ++F  F+ + +G +L + + +K + T +R + ++IR YD +       D  Q+TL CCG  S  D+  S  L    NVP SCC +        CN     +N  NAY +GC
Sbjct:   62 VLLVGGIVVALIAFFGCVGASREVKCMLLTYFIIVFLLFVTMLIGGVLGYVFREKLLSTVDREMKSSIRLYDHRKSIRDAWDTTQSTLHCCGVNSWRDW-GSYGL----NVPESCCREIQPGQRFHCNTGADTVNPSNAYVEGC 200
[Close]
BLAST of SMED30024436 vs. TrEMBL
Match: A0A154P743 (Tetraspanin (Fragment) OS=Dufourea novaeangliae OX=178035 GN=WN55_08433 PE=3 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 3.054e-7
Identity = 39/139 (28.06%), Postives = 65/139 (46.76%), Query Frame = 2 
Query:  227 GIFMIILAIYSLFLVSKNHICQL--FISVIFYFFMAITLGAMLFHTWYDKQIYTKNR-VVTAIRSYDWKSFNSHHLDRLQNTLSCCGYESAADYHQSNNLFLKDNVPLSCCFD-NSKSIIKCNPNVTKINIDNAYPQGC 631
            GI + I++ +     S+   C L  +  ++F  F+ + +G +L + + +K + T  R + +++R YD +       D  Q+TL CCG     D+       L  NVP SCC +        CN     +N  NAY +GC
Sbjct:   53 GIIVAIISFFGCIGASREVKCMLLTYFIIVFLLFVTMLIGGVLAYVFREKLVDTLQREMSSSMRVYDSRKSVREAWDTTQSTLHCCGVSGWRDWGS-----LGLNVPQSCCREIQPGQRFNCNAGPDTVNPSNAYLEGC 186
[Close]
BLAST of SMED30024436 vs. TrEMBL
Match: A0A310S9U4 (Tetraspanin OS=Eufriesea mexicana OX=516756 GN=WN48_09661 PE=3 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 1.115e-6
Identity = 40/139 (28.78%), Postives = 66/139 (47.48%), Query Frame = 2 
Query:  227 GIFMIILAIYSLFLVSKNHICQL--FISVIFYFFMAITLGAMLFHTWYDKQIYTKNR-VVTAIRSYDWKSFNSHHLDRLQNTLSCCGYESAADYHQSNNLFLKDNVPLSCCFD-NSKSIIKCNPNVTKINIDNAYPQGC 631
            GI + ++A +     S+   C L  +  ++F  F+ + +G +L + + +K + T  R + +++R YD +       D  Q+TL CCG     D+    NL L  NVP SCC +        CN    ++N  N Y  GC
Sbjct:   67 GIIVALVAFFGCVGASREVKCMLLTYFIILFLLFVTMLIGGVLAYVFREKLVSTLEREMSSSMRVYDTRKVVKEAWDTTQSTLHCCGVNGWRDW---GNLGL--NVPESCCREIQPGQRFNCNTGPDRVNSANTYLAGC 200
[Close]
BLAST of SMED30024436 vs. TrEMBL
Match: A0A4S2JAV6 (Tetraspanin OS=Temnothorax longispinosus OX=300112 GN=DBV15_05403 PE=3 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 1.248e-6
Identity = 39/144 (27.08%), Postives = 69/144 (47.92%), Query Frame = 2 
Query:  212 LVIIVGIFMIILAIYSLFLVSKNHICQL--FISVIFYFFMAITLGAMLFHTWYDKQIYTKNRVV-TAIRSYDWKSFNSHHLDRLQNTLSCCGYESAADYHQSNNLFLKDNVPLSCCFD-NSKSIIKCNPNVTKINIDNAYPQGC 631
            ++++ GI +  +A +     S+   C L  +  ++F  F+ + +G +L + + +K + T +R + +++R YD +       D  Q+TL CCG  S  D+          NVP SCC +        CN     +N  NAY +GC
Sbjct:   62 VLLVGGIVVAFIAFFGCVGASREVKCMLLTYFIIVFLLFVTMLIGGVLGYVFREKLLGTLDREMKSSLRLYDHRKSIRDAWDTTQSTLHCCGVNSWRDWGS-----FGLNVPESCCREIQPGQRFHCNAGPDTVNPSNAYVEGC 200
[Close]
BLAST of SMED30024436 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005284.1 (pep scaffold:Pmarinus_7.0:GL480552:16638:25636:-1 gene:ENSPMAG00000004798.1 transcript:ENSPMAT00000005284.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 48.521 bits (114), Expect = 7.198e-7
Identity = 43/163 (26.38%), Postives = 74/163 (45.40%), Query Frame = 2 
Query:  212 LVIIVGIFMIILAIY--------SLFLVSKNHICQLFISVIFYFFMAITLGAMLFHTWYDKQIYTKNRVVTAIRSYDWKSFNSHHLDRLQNTLSCCGYESAADYHQSNNLFLKD-----NVPLSCCFDNSKSIIKCNPNVTKINIDNA----YPQGCRKQLDS 649
            ++I VG+ + ++A +        S F+V+   +   F+ +IF   +A  +GA +F       + TK +VV      D+ + N   +D +Q  L CCG     D+  S N    D     +VP SCC + +     C  +   I   NA    Y +GC ++L++
Sbjct:   63 VIIAVGVVIFVVAAFGCCGAHKESYFMVATFSV---FLFLIFVAELAAGIGAYIFKDQLRDVVETKAKVVI----NDYPTKNKDPVDNMQKELQCCGVVHYQDWFNSTNWVTSDPNNPNSVPDSCCLNVTSG---CGQD--DIRFKNATAIIYTKGCVEKLEA 213
[Close]
BLAST of SMED30024436 vs. Ensembl Sea Lamprey
Match: tspan3a (tetraspanin 3a [Source:ZFIN;Acc:ZDB-GENE-030131-7787])

HSP 1 Score: 46.2098 bits (108), Expect = 3.640e-6
Identity = 42/162 (25.93%), Postives = 73/162 (45.06%), Query Frame = 2 
Query:  212 LVIIVGIFMIILAIYSLFLVSKNHICQL---FISVIFYFFMAITLGAMLFHTWYDKQIYTK-NRVVTAI-RSYDWKSFN--SHHLDRLQNTLSCCGYESAADY-------HQSNNLFLKDNVPLSCCFDNSKSIIKCNPNVTKINIDNAYPQGCRKQLDSIV 655
            +V+ VGIF+ ++ I       +   C L   FI ++  F + +   A L    Y  Q+  K  R ++ +   Y+    N  S  +D +Q+ L CCG ++  D+       H  NN     +VP+SCC  N   +  C+ N+   +  + Y +GC   ++  V
Sbjct:   58 VVVAVGIFLFVVGIVGCCATVRESKCGLSTFFILLLMIFSLEVV--AALLGYAYRGQVKDKVERTISQVFNEYNGTDSNTASRAIDYVQHQLKCCGVKNYLDWKDRPWFNHTGNN-----SVPISCCRSN---VTHCDGNIK--HPGDIYNEGCEAMVEKTV 207
[Close]
BLAST of SMED30024436 vs. Ensembl Nematostella
Match: EDO39846 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S900])

HSP 1 Score: 55.8398 bits (133), Expect = 1.836e-9
Identity = 34/116 (29.31%), Postives = 55/116 (47.41%), Query Frame = 2 
Query:  212 LVIIVGIFMIILAIYSLFLVSKNHICQL---FISVIFYFFMAITLGAMLFHTWYDKQIYTKNRVVTAIRSYDWKSFNSHHLDRLQNTLSCCGYESAADYHQSNNLFLKDNVPLSCC 550
            ++I  G  + I+A        K   C L   F+ ++  F + I    + F      +   +N +   I+SY   + N+  +D +Q T+ CCG  +  D+  S+NL L DNVP SCC
Sbjct:   51 IIIAAGCVVTIVAFLGCCGAWKESKCMLICFFVCMLILFCLEIAAAVLAFVKKDKVKDELENELKNQIKSYTGSARNA--VDDMQKTVKCCGITTYEDWKSSDNLGLTDNVPDSCC 164
[Close]
BLAST of SMED30024436 vs. Ensembl Nematostella
Match: EDO41431 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S485])

HSP 1 Score: 49.6766 bits (117), Expect = 5.701e-7
Identity = 40/153 (26.14%), Postives = 64/153 (41.83%), Query Frame = 2 
Query:  221 IVGIFMIILAIYSLFLVSKNHICQLFI---SVIFYFFMAITLGAMLFHTWYDKQIYTKNRVVTAIRSYDWKSFNSHHLDRLQNTLSCCGYESAADYHQSNNLF-------LKDNVPLSCCFDNSKSIIKCNPNVTKINID------NAYPQGC 631
            ++GI M  +  +      + + C L +   SV     + I  GA+  +T  DK    K R+   ++ Y     N   +D +Q  L CCG ES  D+ Q+N  F       +   VP SCC ++ +   +C  +  K+  D        Y  GC
Sbjct:   59 VIGILMFFIGFFGCVGALRENTCFLTVYGTSVFLCLILLIACGAVA-YTQKDKVQALKERLKQDLKKYLDDPLNQDLIDFMQTLLKCCGVESYKDW-QANPYFNCSSPGRMSCGVPFSCCKESIQFNRQCGYDAGKLKTDAERLENGVYSGGC 209
[Close]
BLAST of SMED30024436 vs. Ensembl Nematostella
Match: EDO41432 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S486])

HSP 1 Score: 45.4394 bits (106), Expect = 9.520e-6
Identity = 32/121 (26.45%), Postives = 49/121 (40.50%), Query Frame = 2 
Query:  308 IFYFFMAITLGAMLFHTWYDKQIYTKNRVVTAIRSYDWKSFNSHHLDRLQNTLSCCGYESAADYHQSNNLF-------LKDNVPLSCCFDNSKSIIKCNPNVTKINID------NAYPQGC 631
            IF   + I    +L     +K +  K R+   ++SY   S     +D +Q    CCG ES  D+ Q+N  F           VP SCC  + +   +C  +  K+  D        Y +GC
Sbjct:   38 IFCCLIPIIAAGVLAFLQREKLVDPKARLEDGLKSYQQDSSKQEFIDFIQTRFKCCGIESYKDW-QANRYFSCSSPVPTACGVPFSCCKKSIQGNRQCGYDAGKLKTDADRLENGVYSKGC 157
[Close]
BLAST of SMED30024436 vs. Ensembl Medaka
Match: tspan7 (tetraspanin 6 [Source:NCBI gene;Acc:101163337])

HSP 1 Score: 52.373 bits (124), Expect = 1.856e-7
Identity = 35/171 (20.47%), Postives = 78/171 (45.61%), Query Frame = 2 
Query:  173 NFDQYLNIRQCYG-----LVIIVGIFMIILAIYSLFLVSKN-----HICQLFISVIFYFFMAITLGAMLFHTWYDKQIYTKNRVVTAIRSYDWKSFNSHHLDRLQNTLSCCGYESAADYHQSNNLFLKDNVPLSCCFDNSKSIIKCNPNVTKINIDNAYPQGCRKQLDSIV 655
            + + YL++    G     ++I  G  ++I  ++  F   +       +  +F++++F   +   +   +F   ++ +         A+RSY+  +  S  LD +Q TL CCG ++  D+ ++   F+   +PLSCC DN    ++   ++ K      +  GC   + +++
Sbjct:   45 SLEAYLSLASEGGTNAPYVLIGTGAIIVIFGLFGCFATCRGSPWMLKLYAMFLTLVFLSELVAGISGFIFR--HEIKAVMGTAYENAVRSYNGTNSLSVALDTIQRTLRCCGVKNYTDWKEA-PYFISKGIPLSCCKDNGNCTVEIMKDLDKAG-KEVFTTGCFALVTTVM 211
[Close]
BLAST of SMED30024436 vs. Ensembl Medaka
Match: tspan7 (tetraspanin 6 [Source:NCBI gene;Acc:101163337])

HSP 1 Score: 51.9878 bits (123), Expect = 2.144e-7
Identity = 35/171 (20.47%), Postives = 78/171 (45.61%), Query Frame = 2 
Query:  173 NFDQYLNIRQCYG-----LVIIVGIFMIILAIYSLFLVSKN-----HICQLFISVIFYFFMAITLGAMLFHTWYDKQIYTKNRVVTAIRSYDWKSFNSHHLDRLQNTLSCCGYESAADYHQSNNLFLKDNVPLSCCFDNSKSIIKCNPNVTKINIDNAYPQGCRKQLDSIV 655
            + + YL++    G     ++I  G  ++I  ++  F   +       +  +F++++F   +   +   +F   ++ +         A+RSY+  +  S  LD +Q TL CCG ++  D+ ++   F+   +PLSCC DN    ++   ++ K      +  GC   + +++
Sbjct:   45 SLEAYLSLASEGGTNAPYVLIGTGAIIVIFGLFGCFATCRGSPWMLKLYAMFLTLVFLSELVAGISGFIFR--HEIKAVMGTAYENAVRSYNGTNSLSVALDTIQRTLRCCGVKNYTDWKEA-PYFISKGIPLSCCKDNGNCTVEIMKDLDKAG-KEVFTTGCFALVTTVM 211
[Close]
BLAST of SMED30024436 vs. Planmine SMEST
Match: SMESG000043617.1 (SMESG000043617.1)

HSP 1 Score: 454.521 bits (1168), Expect = 1.060e-161
Identity = 262/265 (98.87%), Postives = 263/265 (99.25%), Query Frame = 2 
Query:    2 MCESECTKRFFDWFLSCRNVFKLLTIILNVTXXXXXXXXXXXXXXXXXSVMVYDLVNNFDQYLNIRQCYGLVIIVGIFMIILAIYSLFLVSKNHICQLFISVIFYFFMAITLGAMLFHTWYDKQIYTKNRVVTAIRSYDWKSFNSHHLDRLQNTLSCCGYESAADYHQSNNLFLKDNVPLSCCFDNSKSIIKCNPNVTKINIDNAYPQGCRKQLDSIVWYXXXXXXXXXXXXXXXXXXXMGLLIILEHCSKKTCENTEKTGDIDV 796
            MCESECTKRFFDWFLSCRNVFKLLTIILNVTICFVGFGIV+TFGIGICSVMVYDLVNNFDQYLNIRQCYGLVIIVGIFMIILAIYSLFLVSKNHICQLFISVIFYFFMAITLGAMLFHTWYDKQIYTKNRVVTAIRSYDWKSFNSHHLDRLQNTLSCCGYE AADYHQSNNLFLKDNVPLSCCFDNSKSIIKCNPNVTKINIDNAYP GCRKQLDSIVWYTIFGIYISLISILFLTFLTMGLLIILEHCSKKTCENTEKTGDIDV
Sbjct:    1 MCESECTKRFFDWFLSCRNVFKLLTIILNVTICFVGFGIVMTFGIGICSVMVYDLVNNFDQYLNIRQCYGLVIIVGIFMIILAIYSLFLVSKNHICQLFISVIFYFFMAITLGAMLFHTWYDKQIYTKNRVVTAIRSYDWKSFNSHHLDRLQNTLSCCGYEGAADYHQSNNLFLKDNVPLSCCFDNSKSIIKCNPNVTKINIDNAYPPGCRKQLDSIVWYTIFGIYISLISILFLTFLTMGLLIILEHCSKKTCENTEKTGDIDV 265
[Close]
BLAST of SMED30024436 vs. Planmine SMEST
Match: SMESG000035080.1 (SMESG000035080.1)

HSP 1 Score: 59.3066 bits (142), Expect = 7.007e-10
Identity = 41/166 (24.70%), Postives = 76/166 (45.78%), Query Frame = 2 
Query:  158 YDLVNNFDQYLNIRQCYGLVIIVGIFMIILAIYSLFLV--SKNHICQLFISVIFYFFMAITLGAMLFHTWYDKQIYTKNRVVTAIRSYDWKSFNSHH---LDRLQNTLSCCGYESAADYHQSNNLFLKDNVPLSCCFDNSKSIIKCNPNVTKINIDNAYPQGCRKQ 640
            +DL  + D Y   ++    ++ +GIF I L++   F        I  ++ SV+ + F +      L+  + D+++ T  ++  +I++Y            L+ +Q  L CCG +S  DY + +      N+P SCC      I +C   V K    NA+ +GC+ +
Sbjct:   41 HDLTRSPDHYTEFKKILAFIMGIGIFTIALSLVGCFGAYHESQPILGIYFSVMLFLFASQVAVGSLYFVYKDEKLTTNLKI--SIQNYMNSKITKQEEIFLNSVQKMLKCCGVDSVNDYIKKDT---PSNIPFSCC---DYEIHECKAKVEK---KNAFTKGCKDK 195
[Close]
BLAST of SMED30024436 vs. Planmine SMEST
Match: SMESG000051756.1 (SMESG000051756.1)

HSP 1 Score: 53.1434 bits (126), Expect = 3.241e-8
Identity = 36/114 (31.58%), Postives = 53/114 (46.49%), Query Frame = 2 
Query:  341 AMLFHTWYDKQIYTKNRVVTAIRSYDWKSFNSHHLDRLQNTLSCCGYESAADYHQSNNLFLKDNVPLSCCFDNSKSII----KCNPNVTK----INIDNAYPQGCRKQLDSIVW 658
            A++F  W + +      +  A + YD    +S + + +Q  L CCG +S  D+   N  F    VP+SCC      II    K   N+ K    INI  A+  GC  +L+ IVW
Sbjct:    3 AIIFTFWVEWEANAHFGISYATQIYDPNKTHSEYYEIIQKFLRCCGAKSPKDFDIGNEYFKLGTVPISCCNFQENPIICSQMKNLKNLGKGSLAINIKYAFQTGCLTRLEKIVW 116
[Close]
BLAST of SMED30024436 vs. Planmine SMEST
Match: SMESG000039913.1 (SMESG000039913.1)

HSP 1 Score: 50.447 bits (119), Expect = 7.403e-7
Identity = 33/130 (25.38%), Postives = 64/130 (49.23%), Query Frame = 2 
Query:  290 QLFISVIFYFFMAITLGAMLFHTWYDKQIYTKNRVVTAIRSYDWKSFNSHHLDRLQNTLSCCGYESAADYHQSNNLFLKDNVPLSCC--FDNSKSIIK-----CNPNVTKINIDNAYPQGCRKQLDSIVW 658
            Q  +  IF+  + I +G     + Y   IY    +  A+  Y+ KS +S +++ ++  L CCG     DY+ + +LF  + VP SCC  +  +++ +K        N  +I I+ A+  GC ++   +++
Sbjct:  106 QTILLSIFWIVLLILIGT----SIYGYHIYIDYGLTFAVNMYNPKSPSSQYMEVIEKHLQCCGNLGPKDYN-NRDLFDSNTVPFSCCDYYHKNRTTVKNLCKFFEVNYKRIPIELAFQTGCLEKFHDVIF 230
[Close]
BLAST of SMED30024436 vs. Planmine SMEST
Match: SMESG000039913.1 (SMESG000039913.1)

HSP 1 Score: 49.6766 bits (117), Expect = 1.001e-6
Identity = 33/130 (25.38%), Postives = 64/130 (49.23%), Query Frame = 2 
Query:  290 QLFISVIFYFFMAITLGAMLFHTWYDKQIYTKNRVVTAIRSYDWKSFNSHHLDRLQNTLSCCGYESAADYHQSNNLFLKDNVPLSCC--FDNSKSIIK-----CNPNVTKINIDNAYPQGCRKQLDSIVW 658
            Q  +  IF+  + I +G  +    Y   IY    +  A+  Y+ KS +S +++ ++  L CCG     DY+ + +LF  + VP SCC  +  +++ +K        N  +I I+ A+  GC ++   +++
Sbjct:   27 QTILLSIFWIVLLILIGTSI----YGYHIYIDYGLTFAVNMYNPKSPSSQYMEVIEKHLQCCGNLGPKDYN-NRDLFDSNTVPFSCCDYYHKNRTTVKNLCKFFEVNYKRIPIELAFQTGCLEKFHDVIF 151
The following BLAST results are available for this feature:
BLAST of SMED30024436 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024436 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024436 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024436 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024436 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024436 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024436 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024436 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A151WM121.793e-729.66Tetraspanin OS=Trachymyrmex zeteki OX=64791 GN=ALC... [more]
A0A026W8X02.790e-729.17Tetraspanin OS=Ooceraea biroi OX=2015173 GN=DMN91_... [more]
A0A154P7433.054e-728.06Tetraspanin (Fragment) OS=Dufourea novaeangliae OX... [more]
A0A310S9U41.115e-628.78Tetraspanin OS=Eufriesea mexicana OX=516756 GN=WN4... [more]
A0A4S2JAV61.248e-627.08Tetraspanin OS=Temnothorax longispinosus OX=300112... [more]
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BLAST of SMED30024436 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024436 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000005284.17.198e-726.38pep scaffold:Pmarinus_7.0:GL480552:16638:25636:-1 ... [more]
tspan3a3.640e-625.93tetraspanin 3a [Source:ZFIN;Acc:ZDB-GENE-030131-77... [more]
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BLAST of SMED30024436 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024436 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3
Match NameE-valueIdentityDescription
EDO398461.836e-929.31Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO414315.701e-726.14Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO414329.520e-626.45Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30024436 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
tspan71.856e-720.47tetraspanin 6 [Source:NCBI gene;Acc:101163337][more]
tspan72.144e-720.47tetraspanin 6 [Source:NCBI gene;Acc:101163337][more]
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BLAST of SMED30024436 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000043617.11.060e-16198.87SMESG000043617.1[more]
SMESG000035080.17.007e-1024.70SMESG000035080.1[more]
SMESG000051756.13.241e-831.58SMESG000051756.1[more]
SMESG000039913.17.403e-725.38SMESG000039913.1[more]
SMESG000039913.11.001e-625.38SMESG000039913.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024436 ID=SMED30024436|Name=SMED30024436|organism=Schmidtea mediterranea sexual|type=transcript|length=1028bp
CATGTGTGAATCGGAGTGCACGAAAAGGTTTTTCGACTGGTTTTTGTCTT
GTAGAAATGTTTTCAAATTGTTGACGATCATTCTAAACGTTACGATCTGT
TTCGTTGGTTTCGGAATCGTCATAACATTTGGGATCGGGATTTGCAGTGT
CATGGTTTATGATTTGGTGAACAATTTCGATCAGTATTTAAATATCCGCC
AATGTTACGGTCTGGTGATCATTGTAGGAATATTTATGATTATTCTGGCG
ATATATTCGCTGTTTTTAGTTTCGAAAAATCATATTTGTCAGTTATTTAT
TTCGGTTATATTTTACTTCTTTATGGCTATTACGCTCGGGGCGATGTTGT
TTCATACTTGGTATGATAAACAGATTTACACAAAAAATCGGGTGGTCACT
GCTATTCGTTCGTACGACTGGAAAAGTTTCAATTCACATCATTTGGATCG
TTTGCAAAATACTCTTAGTTGTTGTGGCTATGAAAGTGCAGCTGACTACC
ACCAAAGCAATAACCTATTCTTGAAAGACAATGTTCCGCTATCTTGCTGC
TTTGATAATTCAAAATCTATCATCAAATGTAACCCAAATGTCACCAAAAT
AAATATAGACAATGCCTATCCCCAAGGATGCCGTAAGCAGCTCGACTCAA
TAGTCTGGTATACAATTTTCGGAATTTATATCAGCTTGATATCAATTCTA
TTTCTAACATTTCTGACAATGGGATTATTAATAATCTTAGAACATTGTTC
AAAAAAAACTTGTGAAAACACTGAGAAAACTGGTGACATCGATGTTGAGA
ACAAATCGGAAAATCAAGATGAGATCGAGGAAGAAGAAGAAACTAAAGAA
GGCGATCTTGAAAATGAATATGTTGAAGAGAGTGAAAATGAAAATGAAAA
AGAAGAAAACGACAACGTGAATGGGAGCAATCAAGCAAAAGAATGACTGT
AAAATCGATCTTTAATTTCCTCCAATTATAAATTTATTTACTAAAATATT
AACTATTTAATTGTTTTTCAAAAAAAAA
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protein sequence of SMED30024436-orf-1

>SMED30024436-orf-1 ID=SMED30024436-orf-1|Name=SMED30024436-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=315bp
MCESECTKRFFDWFLSCRNVFKLLTIILNVTICFVGFGIVITFGIGICSV
MVYDLVNNFDQYLNIRQCYGLVIIVGIFMIILAIYSLFLVSKNHICQLFI
SVIFYFFMAITLGAMLFHTWYDKQIYTKNRVVTAIRSYDWKSFNSHHLDR
LQNTLSCCGYESAADYHQSNNLFLKDNVPLSCCFDNSKSIIKCNPNVTKI
NIDNAYPQGCRKQLDSIVWYTIFGIYISLISILFLTFLTMGLLIILEHCS
KKTCENTEKTGDIDVENKSENQDEIEEEEETKEGDLENEYVEESENENEK
EENDNVNGSNQAKE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR018499Tetraspanin/Peripherin
IPR008952Tetraspanin_EC2_sf
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 291..314
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 261..314
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 269..300
NoneNo IPR availablePANTHERPTHR19282TETRASPANINcoord: 39..240
NoneNo IPR availablePANTHERPTHR19282:SF252coord: 39..240
NoneNo IPR availableTMHMMTMhelixcoord: 69..91
NoneNo IPR availableTMHMMTMhelixcoord: 23..45
NoneNo IPR availableTMHMMTMhelixcoord: 224..246
NoneNo IPR availableTMHMMTMhelixcoord: 98..120
IPR008952Tetraspanin, EC2 domain superfamilyGENE3DG3DSA:1.10.1450.10coord: 122..216
e-value: 2.7E-8
score: 35.5
IPR008952Tetraspanin, EC2 domain superfamilySUPERFAMILYSSF48652Tetraspanincoord: 144..217
IPR018499Tetraspanin/PeripherinPFAMPF00335Tetraspanincoord: 22..246
e-value: 3.5E-18
score: 66.2