mitochondrial import receptor subunit TOM22 homolog
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30024389 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. Ensembl Celegans
Match: tomm-22 (Mitochondrial import receptor subunit TOM22 homolog [Source:UniProtKB/Swiss-Prot;Acc:O17287]) HSP 1 Score: 43.8986 bits (102), Expect = 6.877e-6 Identity = 22/80 (27.50%), Postives = 42/80 (52.50%), Query Frame = 3 Query: 171 ERIIGLTEMLPESVRSLTSKSASRSWKIIKKSYTLFRNAGWILASVLVVGAGPAVFENLRDKALAEDADQQRKLMFGPQS 410 ERI GL EM P+++RS + S +K ++L ++ W++++ ++ P + E R QQR+++ GP + Sbjct: 22 ERIEGLGEMFPDALRSAVHSTVDWSIWGVKGVFSLTKSTIWVVSTTSLIAFLPYIIEKERSDLEKTQVAQQRQMLLGPSA 101
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. Ensembl Fly
Match: mge (gene:FBgn0035473 transcript:FBtr0073175) HSP 1 Score: 56.6102 bits (135), Expect = 3.507e-10 Identity = 34/95 (35.79%), Postives = 47/95 (49.47%), Query Frame = 3 Query: 171 ERIIGLTEMLPESVRSLTSKSASRSWKIIKKSYTLFRNAGWILASVLVVGAGPAVFENLRDKALAEDADQQRKLMFGPQSESGFAIP----PTIR 443 ER GLTEM PE VR+ +S + K +K Y+ NA WI + V+ P +FE R + QQ++++ GP S G P P IR Sbjct: 28 ERFWGLTEMFPEPVRNAVGAVSSATVKSVKGFYSFSCNASWIFFTSAVILFAPVIFETERAQMEELHKSQQKQVLLGPGSAMGPGGPSPSLPLIR 122
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. Ensembl Fly
Match: mge (gene:FBgn0035473 transcript:FBtr0073176) HSP 1 Score: 55.8398 bits (133), Expect = 1.126e-9 Identity = 34/95 (35.79%), Postives = 47/95 (49.47%), Query Frame = 3 Query: 171 ERIIGLTEMLPESVRSLTSKSASRSWKIIKKSYTLFRNAGWILASVLVVGAGPAVFENLRDKALAEDADQQRKLMFGPQSESGFAIP----PTIR 443 ER GLTEM PE VR+ +S + K +K Y+ NA WI + V+ P +FE R + QQ++++ GP S G P P IR Sbjct: 54 ERFWGLTEMFPEPVRNAVGAVSSATVKSVKGFYSFSCNASWIFFTSAVILFAPVIFETERAQMEELHKSQQKQVLLGPGSAMGPGGPSPSLPLIR 148
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. Ensembl Zebrafish
Match: tomm22 (translocase of outer mitochondrial membrane 22 homolog (yeast) [Source:ZFIN;Acc:ZDB-GENE-030131-9810]) HSP 1 Score: 45.4394 bits (106), Expect = 6.064e-6 Identity = 25/61 (40.98%), Postives = 33/61 (54.10%), Query Frame = 3 Query: 162 NLWERIIGLTEMLPESVRSLTSKSASRSWKIIKKSYTLFRNAGWILASVLVVGAGPAVFEN 344 + ER+ GLTEM P+SVRS SA S KK Y+ R A W+ + ++ P VFE Sbjct: 35 TMLERLWGLTEMFPDSVRSAAEVSAHCSLSAAKKLYSFSRAALWVGTTSFMILVLPVVFET 95
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. Ensembl Zebrafish
Match: tomm22 (translocase of outer mitochondrial membrane 22 homolog (yeast) [Source:ZFIN;Acc:ZDB-GENE-030131-9810]) HSP 1 Score: 45.4394 bits (106), Expect = 6.064e-6 Identity = 25/61 (40.98%), Postives = 33/61 (54.10%), Query Frame = 3 Query: 162 NLWERIIGLTEMLPESVRSLTSKSASRSWKIIKKSYTLFRNAGWILASVLVVGAGPAVFEN 344 + ER+ GLTEM P+SVRS SA S KK Y+ R A W+ + ++ P VFE Sbjct: 35 TMLERLWGLTEMFPDSVRSAAEVSAHCSLSAAKKLYSFSRAALWVGTTSFMILVLPVVFET 95
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. Ensembl Xenopus
Match: b3galt5.4 (beta-1,3-galactosyltransferase 5 gene 4 [Source:Xenbase;Acc:XB-GENE-1013344]) HSP 1 Score: 46.2098 bits (108), Expect = 4.378e-6 Identity = 32/91 (35.16%), Postives = 47/91 (51.65%), Query Frame = 3 Query: 162 NLWERIIGLTEMLPESVRSLTSKSASRSWKIIKKSYTLFRNAGWILASVLVVGAGPAVFENLRDKALAEDADQQRKLMFGPQSESGFAIPP 434 L ER+ GLTEM PES+R+ S S KK Y+ R A WI + ++ P VFE + + + QQR+++ GP + +PP Sbjct: 31 TLSERLWGLTEMFPESLRTAAGASMDLSICAAKKFYSFSRTALWIGTTSFMILVLPVVFETEKLQMEQQQQLQQRQILLGPSTGMSGPLPP 121
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. TrEMBL
Match: A0A2H1BSF2 (Translocase of outer mitochondrial membrane 22 OS=Fasciola hepatica OX=6192 GN=D915_010995 PE=4 SV=1) HSP 1 Score: 65.855 bits (159), Expect = 1.187e-9 Identity = 34/82 (41.46%), Postives = 48/82 (58.54%), Query Frame = 3 Query: 162 NLWERIIGLTEMLPESVRSLTSKSASRSWKIIKKSYTLFRNAGWILASVLVVGAGPAVFENLRDKALAEDADQQRKLMFGPQ 407 L ER++GLTEM PESVR S +AS ++ IK +Y+L R+ W LAS + P E R + ++A QR +M GP+ Sbjct: 70 TLVERLMGLTEMFPESVRRTFSSAASMTFSGIKSTYSLSRSVSWFLASTATLCFLPLFLELERVQTEEQEAVHQRTMMLGPR 151
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. TrEMBL
Match: A0A210Q3C0 (Mitochondrial import receptor subunit TOM22-like OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT06550 PE=4 SV=1) HSP 1 Score: 64.3142 bits (155), Expect = 1.845e-9 Identity = 31/83 (37.35%), Postives = 50/83 (60.24%), Query Frame = 3 Query: 162 NLWERIIGLTEMLPESVRSLTSKSASRSWKIIKKSYTLFRNAGWILASVLVVGAGPAVFENLRDKALAEDADQQRKLMFGPQS 410 L ER+IGLTEM PE+VR++ +A+ + K SY R+A W++AS + P VFE+ R + +Q+R+++ GP + Sbjct: 30 TLRERLIGLTEMFPENVRTVCGNAATFAMAASKASYGFTRSALWVVASAATILTLPVVFESERAQHQEMQREQERQILLGPNA 112
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. TrEMBL
Match: V4CN23 (Uncharacterized protein (Fragment) OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_110138 PE=4 SV=1) HSP 1 Score: 63.1586 bits (152), Expect = 2.761e-9 Identity = 31/83 (37.35%), Postives = 48/83 (57.83%), Query Frame = 3 Query: 162 NLWERIIGLTEMLPESVRSLTSKSASRSWKIIKKSYTLFRNAGWILASVLVVGAGPAVFENLRDKALAEDADQQRKLMFGPQS 410 L ER++GLTEM PE VR + S S+ + K S+TL R W+LAS + P +FE+ + ++ QQR+++ GP + Sbjct: 5 TLSERLMGLTEMFPEGVRKTCGSAVSLSFSLAKTSFTLGRGLLWVLASSATILILPVMFESEMAQMKEQELQQQRQMLLGPNA 87
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. TrEMBL
Match: A0A1B6JPM7 (Uncharacterized protein OS=Homalodisca liturata OX=320908 GN=g.8101 PE=4 SV=1) HSP 1 Score: 62.3882 bits (150), Expect = 8.750e-9 Identity = 36/83 (43.37%), Postives = 46/83 (55.42%), Query Frame = 3 Query: 171 ERIIGLTEMLPESVRSLTSKSASRSWKIIKKSYTLFRNAGWILASVLVVGAGPAVFENLRDKALAEDADQQRKLMFGPQSESG 419 ER+IGLTEM PE V + T A + IK Y L RNA WI AS ++ GP +FE R + QQ++ + GP S SG Sbjct: 45 ERLIGLTEMFPEPVVNFTVSVARTTKAGIKGFYNLSRNALWIAASSSLILFGPLLFEVERAQLEESQRSQQKQFLLGPNSMSG 127
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. TrEMBL
Match: A0A0R3WFH5 (Uncharacterized protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS9619 PE=4 SV=1) HSP 1 Score: 63.1586 bits (152), Expect = 1.168e-8 Identity = 28/81 (34.57%), Postives = 48/81 (59.26%), Query Frame = 3 Query: 162 NLWERIIGLTEMLPESVRSLTSKSASRSWKIIKKSYTLFRNAGWILASVLVVGAGPAVFENLRDKALAEDADQQRKLMFGP 404 L ER+IGLTEM P+ +RS T+ + + ++ +Y+L R + W AS++ V P + E R++ ++A + R +M GP Sbjct: 69 TLMERLIGLTEMFPDRLRSATASAFGGTMGAVRSAYSLLRASAWFAASIMTVCMLPLMLELERNQMEEQEASEHRSMMLGP 149
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. Ensembl Cavefish
Match: tomm22 (translocase of outer mitochondrial membrane 22 [Source:NCBI gene;Acc:103023388]) HSP 1 Score: 45.8246 bits (107), Expect = 3.428e-6 Identity = 23/61 (37.70%), Postives = 35/61 (57.38%), Query Frame = 3 Query: 162 NLWERIIGLTEMLPESVRSLTSKSASRSWKIIKKSYTLFRNAGWILASVLVVGAGPAVFEN 344 + ER+ GLTEM P+++RS SA S + KK Y+ R+A W+ + ++ P VFE Sbjct: 32 TMLERLWGLTEMFPDTLRSAAEVSAQCSLSVAKKLYSFSRSALWVGTTSFMILVLPVVFET 92
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. Ensembl Medaka
Match: tomm22 (translocase of outer mitochondrial membrane 22 [Source:NCBI gene;Acc:101166421]) HSP 1 Score: 47.7506 bits (112), Expect = 8.286e-7 Identity = 25/61 (40.98%), Postives = 36/61 (59.02%), Query Frame = 3 Query: 162 NLWERIIGLTEMLPESVRSLTSKSASRSWKIIKKSYTLFRNAGWILASVLVVGAGPAVFEN 344 L ER+ GLTEM PE+VR+ SA S ++KK Y+ R+A W+ + ++ P VFE Sbjct: 36 TLAERLWGLTEMFPETVRTAAEVSAQCSVSLLKKFYSFSRSALWVGTTSFMILVLPVVFET 96
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. Planmine SMEST
Match: SMESG000027748.1 (SMESG000027748.1) HSP 1 Score: 201.06 bits (510), Expect = 1.800e-66 Identity = 110/110 (100.00%), Postives = 110/110 (100.00%), Query Frame = 3 Query: 120 MSLSIXXXXXXXXXNLWERIIGLTEMLPESVRSLTSKSASRSWKIIKKSYTLFRNAGWILASVLVVGAGPAVFENLRDKALAEDADQQRKLMFGPQSESGFAIPPTIRAN 449 MSLSIEDEELLEDENLWERIIGLTEMLPESVRSLTSKSASRSWKIIKKSYTLFRNAGWILASVLVVGAGPAVFENLRDKALAEDADQQRKLMFGPQSESGFAIPPTIRAN Sbjct: 1 MSLSIEDEELLEDENLWERIIGLTEMLPESVRSLTSKSASRSWKIIKKSYTLFRNAGWILASVLVVGAGPAVFENLRDKALAEDADQQRKLMFGPQSESGFAIPPTIRAN 110 The following BLAST results are available for this feature:
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 1
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 2
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 2
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 1
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 1
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 1
BLAST of mitochondrial import receptor subunit TOM22 homolog vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30024389 ID=SMED30024389|Name=mitochondrial import receptor subunit TOM22 homolog|organism=Schmidtea mediterranea sexual|type=transcript|length=685bpback to top protein sequence of SMED30024389-orf-1 >SMED30024389-orf-1 ID=SMED30024389-orf-1|Name=SMED30024389-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=111bp MSLSIEDEELLEDENLWERIIGLTEMLPESVRSLTSKSASRSWKIIKKSYback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|