Rho_N domain-containing protein

Overview
NameRho_N domain-containing protein
Smed IDSMED30024373
Length (bp)3696
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Rho_N domain-containing protein (SMED30024373) t-SNE clustered cells

Violin plots show distribution of expression levels for Rho_N domain-containing protein (SMED30024373) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Rho_N domain-containing protein (SMED30024373) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Rho_N domain-containing protein (SMED30024373) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Homology
[Close]
BLAST of Rho_N domain-containing protein vs. Ensembl Celegans
Match: ZC53.4 (pep chromosome:WBcel235:X:1925750:1937689:1 gene:WBGene00022511.1 transcript:ZC53.4.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ZC53.4)

HSP 1 Score: 58.5362 bits (140), Expect = 3.128e-19
Identity = 38/103 (36.89%), Postives = 64/103 (62.14%), Query Frame = 2 
Query: 1883 RKNYVXXXXXXXXXXXXGEKVVNIHITIRFKQSPIFKKYLDTNINLRASAVSELCRDLYKLYNNSLYGKTVENLKMRMNLWLLNNSTETLITYSSKLSFRKTI 2191
            R+ YV+H   L   L LG K+  IH  I FK+S     Y++ N N R +A ++  +D YKL NNS++GKT+EN++ R+++ L+ +  + +   +SK +F++ I
Sbjct: 1305 REKYVIHYENLLQCLQLGLKLKKIHRIITFKESNWLASYIEMNTNPRKNAKNDFEKDFYKLMNNSVFGKTMENVRERVDVRLVTDE-DKIKKLASKPNFKRRI 1406

HSP 2 Score: 57.3806 bits (137), Expect = 3.128e-19
Identity = 47/167 (28.14%), Postives = 75/167 (44.91%), Query Frame = 1 
Query: 1465 NIFNNLVQKPLHIMVLNI*VFYGIPGMSW-VMSLTEPIEL--LQDMKMFDFFEGEMLGELTYVNKHYAA-----TSEYIE------LLYIDINNVYGWALSQNLPFSDFKWVHYRMNDVLQESMNEEIEL---------LPHGNTMEVDIDIPDSLH----NFPLAP 1884
            ++F N     L +  L+   ++  PG++W  M     +EL  L D++M+   E  + G +    K Y+      T  + E      LLY+D NN+YGWA+ Q LP  DFK+    +  +L      + +L         L  G   EVD++ P  +H    + PL P
Sbjct: 1119 DVFENFRALSLRVYQLDPCWYFTSPGLAWDAMLKKTKVELDKLHDVEMYLMIEKGIRGGIVNAVKRYSKVNNKYTKNFNEKQLTNFLLYLDANNLYGWAMCQMLPTGDFKFETQSLISILNGEAEVKSDLKDYINYLKKLDRGCIFEVDLEYPKEIHSRHNDSPLCP 1285
[Close]
BLAST of Rho_N domain-containing protein vs. Ensembl Celegans
Match: H12I13.3 (pep chromosome:WBcel235:II:6071386:6073251:-1 gene:WBGene00019192.1 transcript:H12I13.3.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:H12I13.3)

HSP 1 Score: 63.5438 bits (153), Expect = 6.073e-10
Identity = 36/99 (36.36%), Postives = 61/99 (61.62%), Query Frame = 2 
Query: 1934 GEKVVNIHITIRFKQSPIFKKYLDTNINLRASAVSELCRDLYKLYNNSLYGKTVENLKMRMNLWLLNNSTETLITYSSKLSFRKTIKIDENLFAAHLNK 2230
            G K+  IH  + FK+S   KKY+  N NLR  A ++  +D +KL NNS++GKT+EN++ R+N+ L+ +  ++ +    K +F+ +  I E+L +  L K
Sbjct:  299 GLKLKKIHRILTFKESAWMKKYIMLNTNLRKQAKNDFEKDFFKLMNNSVFGKTMENIRNRVNIRLVTDD-DSALKLIRKPNFKNSTIITEDLLSVELTK 396
[Close]
BLAST of Rho_N domain-containing protein vs. TrEMBL
Match: A0A1Y1MY91 (Uncharacterized protein (Fragment) OS=Photinus pyralis OX=7054 PE=4 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 3.376e-31
Identity = 46/119 (38.66%), Postives = 75/119 (63.03%), Query Frame = 2 
Query: 1883 RKNYVXXXXXXXXXXXXGEKVVNIHITIRFKQSPIFKKYLDTNINLRASAVSELCRDLYKLYNNSLYGKTVENLKMRMNLWLLN--NSTETLITYSSKLSFRKTIKIDENLFAAHLNKL 2233
            ++ YV+H   LK  L+ G  +  +H  ++FKQSP  K Y+D N NLR +A +E  ++L+KL NN+++GKT+EN++ R+++ LL+         +Y SK  F+  +  +ENL A  +NKL
Sbjct:  655 KEKYVIHYRALKQALAHGLVLTKVHRVLKFKQSPWLKSYIDLNTNLRRNAKNEFEKNLFKLMNNAVFGKTMENVRKRLDVKLLSKWEGRYGAESYISKPEFKSCVIFNENLVAVEMNKL 773

HSP 2 Score: 64.3142 bits (155), Expect = 3.376e-31
Identity = 43/135 (31.85%), Postives = 67/135 (49.63%), Query Frame = 1 
Query: 1525 FYGIPGMSWVMSLT---EPIELLQDMKMFDFFEGEMLGELTYV---------NKH---YAATSEYIELLYIDINNVYGWALSQNLPFSDFKWVHYRMNDVLQESMNEEIELLP----HGNTMEVDIDIPDSLHNF 1872
            ++ +PG +W   L    + +ELL D  MF F E  + G L+ V         NK+   Y +    + L+Y DINN YGW++SQ LP+  F+WV            N ++ ++P     G  +EVD++ P  LH+ 
Sbjct:  496 YFTLPGFTWDAMLKHTRQELELLTDQDMFLFIERGIRGGLSQVCSKRRVHANNKYMPKYDSAKPDVYLMYNDINNQYGWSMSQYLPYGGFQWV----------DANIDVTMIPDDANEGYILEVDLEYPKQLHDL 620

HSP 3 Score: 47.7506 bits (112), Expect = 3.376e-31
Identity = 38/131 (29.01%), Postives = 67/131 (51.15%), Query Frame = 3 
Query: 1137 IHVRYYDNFSGKYLGQPVENLI-CLAEFKDNYLSYFII*EDMYHIL-KYGIDEFPYWELNIIPTSTKLPP--IWSNVSEDE------YAKAQLIWNQQNCNTLLDNMLAYLKLDVFILAKYFQQFSTKAIA 1499
            I+ R+ D+F  +++   ++ L   L EF +    +F + ED +++L K GI  + Y +         LPP   + N  ED+      Y +AQ +W++ NCN L   +  Y+K D+ +LA  F+ F +  I+
Sbjct:  359 INFRFIDSF--RFMASSLDKLSSALTEFPNLKSQFFALPEDQFNLLTKKGIMPYDYLDSFTRFDEPCLPPQDAFYNKLEDKPCPRRMYRRAQEVWSKFNCNNLGQYVELYMKTDILLLADVFELFRSSCIS 487

HSP 4 Score: 48.1358 bits (113), Expect = 5.436e-9
Identity = 35/127 (27.56%), Postives = 64/127 (50.39%), Query Frame = 3 
Query:  582 EKSDRNHYTAVSDLNKLTNCHQNPDCPVQIRCEWCPRCLNSFRLDNAFNKHVSLCAKNQMGTTLYTLSDNKDLKFENYSKTITPPYVIYADFESVLPHDVRH--HQIHMPSSAGLLLLNNYTKTAIF 956
            E+S + HY  + +L++L +   +     +  C+   RCL+ +R ++  +KH   C +           + K LKF+N+   I  P+V+YAD ESVL    +   +Q H+P++ G     +Y ++  F
Sbjct:   93 EQSTKFHYVWIKNLSRLVSMQLSKRNGQKYICD---RCLHFYRSEDKLHKHTKDCIQKNDTAIKMPTEEKKMLKFKNFKNKIKAPFVVYADLESVLKPSAKKTAYQQHIPAAVGYYFKCSYDESLSF 216

HSP 5 Score: 44.2838 bits (103), Expect = 5.436e-9
Identity = 26/76 (34.21%), Postives = 40/76 (52.63%), Query Frame = 2 
Query: 1067 SMTFAEELQFQSCSTCHICKKPINTC*I-LR*FLW*IS-----GPACRKFNLSRRIQRQLSVVFHNLRGYVSYFKI 1276
             MT  +E++FQ+ + CHIC++P       +R     I      G AC   N++ +    + VVFHNL GY ++F I
Sbjct:  251 QMTPQQEIEFQTATHCHICEQPFTAGQKKVRDHNHLIPENNFRGAACEICNVNYQDTHTIPVVFHNLSGYDAHFLI 326
[Close]
BLAST of Rho_N domain-containing protein vs. TrEMBL
Match: A0A2L2YKB9 (Uncharacterized protein (Fragment) OS=Parasteatoda tepidariorum OX=114398 PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.560e-30
Identity = 53/160 (33.12%), Postives = 84/160 (52.50%), Query Frame = 1 
Query: 1459 LRNIFNNLVQKPLHIMVLNI*VFYGIPGMSW--VMSLTE-PIELLQDMKMFDFFEGEMLGELTYVNKHYA-ATSEYI----------ELLYIDINNVYGWALSQNLPFSDFKWVHYRMNDVLQESMNEEIELLPHGNTMEVDIDIPDSLH----NFPLAP 1884
            L +IF N  +  +    L+   +Y  PG+SW  ++ +T+  IE+ QD  M  F E    G ++  +K YA A ++++           ++Y+D NN+YGWA+SQ LP  +FKWV  +  D+ Q S +        G  +EVD+  PD+LH    + P AP
Sbjct:  190 LVDIFQNFRKVCMGTYSLDPAWYYTSPGLSWDAMLKITKIEIEIFQDYDMLLFVEKGTRGGISQCSKRYARANNKFMIDYDSSKPDNYIMYLDANNLYGWAMSQPLPLKNFKWVDPQTVDITQISDDNS-----QGYILEVDLLYPDTLHDAHSDLPFAP 344

HSP 2 Score: 68.9366 bits (167), Expect = 1.560e-30
Identity = 44/120 (36.67%), Postives = 69/120 (57.50%), Query Frame = 2 
Query: 1883 RKNYVXXXXXXXXXXXXGEKVVNIHITIRFKQSPIFKKYLDTNINLRASAVSELCRDLYKLYNNSLYGKTVENLKMRMNLWLLNNS--TETLITYSSKLSFRKTIKIDENLFAAHLNKLH 2236
            +K YV+H   LK  L  G +V  +H  ++F Q+P  K Y+D N   R+ + ++  +  YKL NNS++GKT+EN++ R+++ L +N    E LI   +K +F       ENL A H+ K H
Sbjct:  364 KKGYVIHYRNLKQCLKFGLEVKKVHRVLQFDQAPFLKPYIDLNTQKRSLSKNDFEKSFYKLMNNSIFGKTMENIRKRVDVRLCSNGKKAEKLI---AKPNFHDRTIFSENLVAFHMQKTH 480

HSP 3 Score: 39.2762 bits (90), Expect = 1.560e-30
Identity = 21/43 (48.84%), Postives = 25/43 (58.14%), Query Frame = 3 
Query: 1353 VSEDEYAKAQLIWNQQNCNTLLDNMLAYLKLDVFILAKYFQQF 1481
            +++DEYA A  IWN  N  TL D    YLK DV +L   FQ F
Sbjct:  155 ITDDEYAYACEIWNTFNFTTLGDYSDHYLKTDVLLLVDIFQNF 197
[Close]
BLAST of Rho_N domain-containing protein vs. TrEMBL
Match: A0A023EZN1 (Putative dna polymerase type b (Fragment) OS=Triatoma infestans OX=30076 PE=2 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.874e-30
Identity = 74/222 (33.33%), Postives = 111/222 (50.00%), Query Frame = 2 
Query: 1856 IAYITFHLLRKNYVXXXXXXXXXXXXGEKVVNIHITIRFKQSPIFKKYLDTNINLRASAVSELCRDLYKLYNNSLYGKTVENLKMRMNLWLLNNST--ETLITYSSKLSFRKTIKIDENLFAAHLNKLH*---------------TVCTGFIQTTNVSTPTH*IGEI*ETI*LPKNIVTGDIDSFFGELE-------FKARFSFASVDLLDTPNYDPSHPLY 2449
            +A++  H   KNY+ H  LLK  L+ G K++ I+  ++FKQSP    Y++ N  LR  A +E  +DLYKLYNNS++GKT+EN++ R+N+ L +N T  E LI     + +  TI  +E+L A HL K H                + C  F    NV  P + + ++         +   D DSF   ++       FK   S+     LD  NY P+HP +
Sbjct:  772 VAHLGPH---KNYICHYMLLKQALNHGLKLLKINRALQFKQSPWLSPYINHNTELRKKADNEFEKDLYKLYNNSVFGKTMENVRRRLNIRLTSNPTQLEKLIARPDCMDW--TI-FNESLAAVHLAKSHIVFTKATYIGLSVLDISKCHLFDYHYNVMVPRYGMEKL--------KLCYMDTDSFVYLIQTDDLYHDFKEMSSY-----LDLSNYKPNHPCF 974

HSP 2 Score: 61.6178 bits (148), Expect = 1.874e-30
Identity = 44/150 (29.33%), Postives = 78/150 (52.00%), Query Frame = 1 
Query: 1459 LRNIFNNLVQKPLHIMVLNI*VFYGIPGMSW--VMSLTEP-IELLQDMKMFDFFEGEMLGELTYVNKHYA-ATSEYIE----------LLYIDINNVYGWALSQNLPFSDFKWVHYRMNDVLQESMNEEIELLPHGNTMEVDIDIPDSLH 1866
            L +IF N     L    L+   +Y +PG+SW  ++  T+  ++LL D       E  + G +  V + YA A ++Y+           + Y D NN+YG+A+ + LP+ +FKWV    N++  E++ E  ++   G  ++VD++ P+ LH
Sbjct:  605 LADIFENFRNVTLRSHKLDPAHYYTLPGLSWDAMLRFTQCRLQLLTDYDQILMIEAGIRGGICQVTRRYASANNKYMPDFNPLQPSTFISYQDCNNLYGFAMCKYLPYDEFKWVE-EPNNINIETLEEHDDV---GYILDVDVEYPNDLH 750

HSP 3 Score: 38.891 bits (89), Expect = 1.874e-30
Identity = 27/77 (35.06%), Postives = 36/77 (46.75%), Query Frame = 3 
Query: 1290 FPY-----WELNIIPTSTKLPP--------IWSNVSEDEYAKAQLIWNQQNCNTLLDNMLAYLKLDVFILAKYFQQF 1481
            FPY     W+   +   T LPP            +SE++Y  A  +WN  +C TL D    YLK DV +LA  F+ F
Sbjct:  539 FPYEYVSSWD---VLNDTCLPPREAFYSSLTGEGISENDYQHALNVWNSFSCATLGDYSDIYLKTDVLLLADIFENF 612

HSP 4 Score: 24.2534 bits (51), Expect = 1.874e-30
Identity = 13/25 (52.00%), Postives = 16/25 (64.00%), Query Frame = 1 
Query: 2461 VIGKFKDES*GVGYTEWVFLCPKCY 2535
            V+GKFKDES G   T++  L  K Y
Sbjct:  981 VLGKFKDESAGEIITDFAGLRSKMY 1005
[Close]
BLAST of Rho_N domain-containing protein vs. TrEMBL
Match: A0A224XH84 (Putative dna polymerase type b (Fragment) OS=Panstrongylus lignarius OX=156445 PE=4 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 1.180e-29
Identity = 52/118 (44.07%), Postives = 70/118 (59.32%), Query Frame = 2 
Query: 1883 RKNYVXXXXXXXXXXXXGEKVVNIHITIRFKQSPIFKKYLDTNINLRASAVSELCRDLYKLYNNSLYGKTVENLKMRMNLWLL--NNSTETLITYSSKLSFRKTIKIDENLFAAHLNK 2230
            + NY++H   LK  L  G ++  IH  ++F+QS   K Y+D N  LRA A +   +DLYKLYNNS+YGKT+EN++ R N+ L+  N   E LI   +K SF       ENL A HL K
Sbjct:  731 KHNYIVHYVTLKQALKHGLQIEKIHRILKFRQSNWLKSYIDYNATLRAVATNTFDKDLYKLYNNSVYGKTMENIRNRKNIKLVCDNKQLEKLI---AKPSFLDRTLYSENLVAVHLAK 845

HSP 2 Score: 61.2326 bits (147), Expect = 1.180e-29
Identity = 42/130 (32.31%), Postives = 65/130 (50.00%), Query Frame = 1 
Query: 1525 FYGIPGMSWVMSLT---EPIELLQDMKMFDFFEGEMLGELTYVNKHYA-ATSEYI-------ELLYI---DINNVYGWALSQNLPFSDFKWVHYRMNDVLQESMNEEIELLPHGNTMEVDIDIPDSLHNF 1872
            +Y +PG+SW   L      +ELLQD +   F E  + G L  V+  Y  A ++Y+       E  YI   D NN+YG+++++ LP+ +F W+     ++     N E     +G  +EVDID P  LH  
Sbjct:  581 YYTLPGLSWDAMLKFTGVRLELLQDYEQIMFIESGIRGGLCQVSHRYCKANNQYLADYNPNLETTYISYQDCNNLYGFSMTKCLPYGEFTWISSEELNLETADDNGE-----YGYILEVDIDYPKKLHRL 705

HSP 3 Score: 43.5134 bits (101), Expect = 1.180e-29
Identity = 39/129 (30.23%), Postives = 61/129 (47.29%), Query Frame = 3 
Query: 1137 IHVRYYDNFS--GKYLGQPVENLICLAEFKDNYLSYFII*EDMYHILKYGIDEFPY-----W---ELNIIPTSTKL--PPIWSNVSEDEYAKAQLIWNQQNCNTLLDNMLAYLKLDVFILAKYFQQFST 1487
            I VR+ D F      L   V+NL  L E K      F   E++  + + G+  FPY     W   E+  +P+          +++S+++Y  AQ +W+  NC TL D    YL+LD  +LA  F+ F +
Sbjct:  447 ISVRFLDTFRFMPTSLNNLVKNLPQLIEVKK-----FYNFEEVKLVTRKGV--FPYDFVTSWDRLEVTSLPSREDFYNRLTGASISDEDYLHAQKVWSAFNCQTLGDYSDHYLQLDTLLLADCFEHFRS 568
[Close]
BLAST of Rho_N domain-containing protein vs. TrEMBL
Match: A0A4Y2PKA1 (Uncharacterized protein OS=Araneus ventricosus OX=182803 GN=AVEN_114505_1 PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 2.807e-29
Identity = 45/116 (38.79%), Postives = 70/116 (60.34%), Query Frame = 2 
Query: 1883 RKNYVXXXXXXXXXXXXGEKVVNIHITIRFKQSPIFKKYLDTNINLRASAVSELCRDLYKLYNNSLYGKTVENLKMRMNLWLLNNSTETLITYSSKLSFRKTIKIDENLFAAHLNK 2230
            + NYV+H  +LK YL +G K+  +H  + F+QS   KKY+D N  +R  A +E  +D YKL NNS++GK +EN++ R+++     S +    Y +K +F+      ENL A H+NK
Sbjct:  440 KTNYVVHYKILKQYLEMGLKLKKVHRILEFEQSDWLKKYIDLNTEMRKKATNEFEKDFYKLMNNSVFGKAIENVRKRIDIKFC-TSYKQAKRYIAKPNFKGRTIFSENLVAIHMNK 554

HSP 2 Score: 67.0106 bits (162), Expect = 2.807e-29
Identity = 46/160 (28.75%), Postives = 80/160 (50.00%), Query Frame = 1 
Query: 1459 LRNIFNNLVQKPLHIMVLNI*VFYGIPGMSWVMSLTE---PIELLQDMKMFDFFEGEMLGELTYVNKHYA-ATSEYIE----------LLYIDINNVYGWALSQNLPFSDFKWVHYRMNDVLQESMNEEIELLPHGNTMEVDIDIPDSLHNF----PLAP 1884
            L +IF       L    L+   ++  PG+SW   L +    ++L+ D+ M    E  + G ++     YA A ++Y++          L+Y+D NN+YGW +SQ LP   FKW++  + ++L+   + +      G  +EVD++ P  LH++    PLAP
Sbjct:  266 LADIFEKFRDVCLKTYKLDPAWYFTAPGLSWDAMLKKTQIKLDLIHDIDMVLMIEKGIRGGISQCCNRYAKANNKYMKEYDKNKESNYLMYLDANNLYGWTMSQYLPHGGFKWIYPNIGNILKCPDDSK-----KGYILEVDLEYPKELHDYHTDLPLAP 420

HSP 3 Score: 43.1282 bits (100), Expect = 2.807e-29
Identity = 26/81 (32.10%), Postives = 39/81 (48.15%), Query Frame = 3 
Query: 1266 ILKYGIDEFPYWELNIIPTSTKLPPIW--------SNVSEDEYAKAQLIWNQQNCNTLLDNMLAYLKLDVFILAKYFQQFS 1484
            I + G+  + Y +       T+LPPI         SN+ +D+Y  AQ +W   N   L +    Y+K DV ILA  F++F 
Sbjct:  194 ITRKGVYPYDYMDCEEKYEETELPPIKDFYNRLNDSNIKDDDYKHAQNVWKSFNIKNLREYSELYVKTDVLILADIFEKFR 274

HSP 4 Score: 20.0162 bits (40), Expect = 2.807e-29
Identity = 8/14 (57.14%), Postives = 10/14 (71.43%), Query Frame = 3 
Query: 2232 YIRQSVLDLSKQRM 2273
            Y+  S+LDLSK  M
Sbjct:  564 YVGMSILDLSKHLM 577
[Close]
BLAST of Rho_N domain-containing protein vs. Ensembl Nematostella
Match: EDO46109 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RR56])

HSP 1 Score: 72.0182 bits (175), Expect = 4.435e-25
Identity = 48/118 (40.68%), Postives = 73/118 (61.86%), Query Frame = 2 
Query: 1883 RKNYVXXXXXXXXXXXXGEKVVNIHITIRFKQSPIFKKYLDTNINLRASAVSELCRDLYKLYNNSLYGKTVENLKMRMNLWLLNNSTET--LITYSSKLSFRKTIKIDENLFAAHLNK 2230
            +  YV+H   LKLY SLG KV  +H  I F++S   K Y+D N +LRA A ++  +D +KL NNS++GKT+EN++ R+++ L+ N  +   LI   SK +++      ENL A H+ K
Sbjct: 1299 KTKYVVHHETLKLYESLGLKVTKVHRGITFEESGWLKTYIDLNTSLRAKATNDFEKDFFKLMNNSVFGKTMENIRNRVDIRLITNEKQARKLI---SKPNYKHRTIFCENLAAIHMRK 1413

HSP 2 Score: 62.3882 bits (150), Expect = 4.435e-25
Identity = 49/160 (30.63%), Postives = 75/160 (46.88%), Query Frame = 1 
Query: 1459 LRNIFNNLVQKPLHIMVLNI*VFYGIPGMSWVMSLT---EPIELLQDMKMFDFFEGEMLGELTYVN-KHYAATSEYIE----------LLYIDINNVYGWALSQNLPFSDFKWVHYRMNDVLQESMNEEIELLPHGNTMEVDIDIPDSLH----NFPLAP 1884
            L ++F N     L    L+   +Y  PG++W  +L      +ELL D  M   FE  + G ++ ++ +H  A + Y+           + Y+D NN+YGWA+S+ LP   F+W    M D       E  E   H   +EVD+  P  LH    ++PLAP
Sbjct: 1132 LADVFENFRDVCLENYGLDPAWYYTAPGLAWDAALKITRVELELLTDCDMLLMFEEGIRGGVSMISTRHSKANNPYMREYDPNLPTKYITYLDANNLYGWAMSKPLPTHGFRW----MTD------QELKEWGRHPCVVEVDMAYPRHLHDSHDDYPLAP 1281

HSP 3 Score: 53.5286 bits (127), Expect = 6.219e-7
Identity = 34/103 (33.01%), Postives = 53/103 (51.46%), Query Frame = 3 
Query:  564 IYLCLLEKSDRNHYTAVSDLNKLTNCHQNPDCPVQIRCEWCPRCLNSFRLDNAFNKHVSLCAKNQ-MGTTLYTLSDNKDL--KFENYSKTITPPYVIYADFES 863
            I L L+   + +HY  + D+  L +   +     +     C RCLNSFR   A  KH   C+KN+ +   +    D K L  +F N+++ +  P+V+YADFES
Sbjct:  721 INLLLISNGETSHYCWIKDVMSLVSSQIDKYHHTRF---LCFRCLNSFRCKKALEKHYEYCSKNESVRIEMPMDKDGKPLYTRFVNFNRKMRVPFVVYADFES 820
[Close]
BLAST of Rho_N domain-containing protein vs. Ensembl Nematostella
Match: EDO29594 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T371])

HSP 1 Score: 46.9802 bits (110), Expect = 8.504e-13
Identity = 49/173 (28.32%), Postives = 72/173 (41.62%), Query Frame = 3 
Query:  387 YSGLEYDEITKYLQI*KENASLNLAINVLFYT----PIVIIDEVPDSYVFHHPNRSKVTDETPIYLCLLEKSDRNHYTAVSDLNKLTNCHQNPDCPVQIRCEWCPRCLNSFRLDNAFNKHVSLCA----------KNQMGTTLYTLSDNKDLKFENYSKTITPPYVIYADFES 863
            +SG+E+  + +  QI K       AINV  Y     P+++               SK  +   I L  +E  +  HY  + +L KL +     D     R   C RCLN FR   +  KH   C           K++ G  L+T        F+NY++ +  P V+YADFES
Sbjct:  361 WSGIEFPVLQR--QIDKFEKQNPYAINVFGYKGDVYPLIV---------------SKKHEARVINLLHIENDETKHYCWIKNLKKLVS--NQIDKFRHFRF-LCYRCLNPFRCKQSLEKHREYCCNHEEVKPVMPKDKDGNPLHTY-------FKNYTRKMRVPSVVYADFES 506

HSP 2 Score: 46.2098 bits (108), Expect = 8.504e-13
Identity = 24/61 (39.34%), Postives = 36/61 (59.02%), Query Frame = 1 
Query:  217 GRGWRVINILEIDFILTKFSPISRLSGHRFIQTPSAIISKKAIINVKNTND-KCVLYSILA 396
            G GW+   +   D  +  F P   LSG  +I+ PS + SKKAIINVKN +D +C  +++ +
Sbjct:  279 GSGWQFDQVEYFDINIDPFEP---LSGSSYIRLPSKLASKKAIINVKNEDDHECFKWAVTS 336
[Close]
BLAST of Rho_N domain-containing protein vs. Ensembl Nematostella
Match: EDO31141 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SYS4])

HSP 1 Score: 51.9878 bits (123), Expect = 1.165e-12
Identity = 48/168 (28.57%), Postives = 76/168 (45.24%), Query Frame = 3 
Query:  387 YSGLEYDEITKYLQI*KENASLNLAINVLFYT----PIVIIDEVPDSYVFHHPNRSKVTDETPIYLCLLEKSDRNHYTAVSDLNKLTNCHQNPDCPVQIRCEWCPRCLNSFRLDNAFNKHVSLCAKNQMGTTLYTLSDNKD-----LKFENYSKTITPPYVIYADFES 863
            +SG+E+    K  QI K        +NV  Y     P+ I ++ PD+                I L L+   + NHY  + ++ +L +   +     +  C  C RCLNSFR   +  KH S C  N     +   + +KD     +KF+ Y++ +  P+V+YADFES
Sbjct:  349 WSGIEFPVSLK--QIDKFEKQNPYTVNVFGYETKVYPLRISEKDPDN---------------AINLLLISNDETNHYCWIKNMMRLVSTQIDKFHHTRFLC--C-RCLNSFRCKQSLEKH-SECCGNHEAVRIEMPNIDKDGNLPHIKFKKYNRKMRVPFVVYADFES 495

HSP 2 Score: 40.4318 bits (93), Expect = 1.165e-12
Identity = 20/61 (32.79%), Postives = 34/61 (55.74%), Query Frame = 1 
Query:  217 GRGWRVINILEIDFILTKFSPISRLSGHRFIQTPSAIISKKAIINVKNTND-KCVLYSILA 396
            G GW+   +   D  +  F P   LSG  +I  PS + +K A+INV+N +D +C  +++ +
Sbjct:  267 GSGWQFDQVEYFDINIDPFEP---LSGSSYIPLPSKLATKHAVINVRNGDDHECFKWAVTS 324
[Close]
BLAST of Rho_N domain-containing protein vs. Ensembl Nematostella
Match: EDO44110 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RWZ0])

HSP 1 Score: 60.077 bits (144), Expect = 2.481e-9
Identity = 38/117 (32.48%), Postives = 57/117 (48.72%), Query Frame = 3 
Query:  540 SKVTDETPIYLCLLEKSDRNHYTAVSDLNKLTNCHQNPDCPVQIRCEWCPRCLNSFRLDNAFNKHVSLCAKNQM----------GTTLYTLSDNKDLKFENYSKTITPPYVIYADFE 860
            SK  D   I L L+   + NHY  + ++++L +         Q   E+CPRCLNSF    A  +H   C+KN+           G  LYT       KF ++++ +  P+V+YADFE
Sbjct:  136 SKRHDTRTINLLLISDDETNHYCWIKNMSRLLSSQIGDH---QHASEFCPRCLNSFWCKEALERHSEYCSKNEEVKIEMPRDIDGNPLYT-------KFRSFNRKMRVPFVVYADFE 242
[Close]
BLAST of Rho_N domain-containing protein vs. Ensembl Nematostella
Match: EDO48021 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RKP4])

HSP 1 Score: 60.4622 bits (145), Expect = 3.083e-9
Identity = 41/117 (35.04%), Postives = 59/117 (50.43%), Query Frame = 3 
Query:  531 PNR-SKVTDETPIYLCLLEKSDRNHYTAVSDLNKLTNCHQNPDCPVQIRCEWCPRCLNSFRLDNAFNKHVSLCAKNQMGTTLYTLSDNKD-----LKFENYSKTITPPYVIYADFES 863
            P R SK  +   I L L+   + NHY  + D+++L +   N     Q   E+CPRCLN F    A  KH   C  N     +     +KD     +KF+NY++ +  P+V+YADFES
Sbjct:  328 PRRISKKRNARVINLLLISNDETNHYCWIKDMSRLLSSQINNH---QHATEFCPRCLNPFWCKKALEKHSEYC-DNHEAVRIEMPKIDKDGNLPHIKFKNYNRKMRVPFVVYADFES 440

HSP 2 Score: 50.0618 bits (118), Expect = 4.840e-6
Identity = 26/61 (42.62%), Postives = 36/61 (59.02%), Query Frame = 1 
Query:  217 GRGWRVINILEIDFILTKFSPISRLSGHRFIQTPSAIISKKAIINVKNTND-KCVLYSILA 396
            G GWR   +   D  +  F P   LSG  +IQ PS + SK+AIINVKN ND +C  +++ +
Sbjct:  212 GSGWRFDQVEYFDINIDPFEP---LSGSSYIQLPSKLASKQAIINVKNKNDHECFKWAVTS 269
[Close]
BLAST of Rho_N domain-containing protein vs. Planmine SMEST
Match: SMESG000040848.1 (SMESG000040848.1)

HSP 1 Score: 258.07 bits (658), Expect = 0.000e+0
Identity = 130/198 (65.66%), Postives = 152/198 (76.77%), Query Frame = 3 
Query:  390 SGLEYDEIT-------KYLQI*KENASLNLAINVLFYTPIVIIDEVPDSYVFHHPN-----RSKVTDETPIYLCLLEKSDRNHYTAVSDLNKLTNCHQNPDCPVQIRCEWCPRCLNSFRLDNAFNKHVSLCAKNQMGTTLYTLSDNKDLKFENYSKTITPPYVIYADFESVLPHDVRHHQIHMPSSAGLLLLNNYTKT 947
            SG +YDEIT          ++ K+N++L LAINV  YTP VIID+VPD+YVFHH +     RSKVTD  P YL LLE +DR HYTAV+++ +L NCHQ PDC +QIR  WCP CLN FRLDNAFN HV+LCAKNQ GTTLYTL +NK+L+F NYSK ITPP+VIYADFESVLP D RHHQIH P +AGLLL+NNYT T
Sbjct:  117 SGDKYDEITDCPMKLINIPKVEKKNSTLKLAINVSSYTPTVIIDKVPDNYVFHHSHLSLIHRSKVTDGNPFYLLLLENNDRYHYTAVTNIERLMNCHQTPDCNIQIRYRWCPHCLNGFRLDNAFNTHVALCAKNQTGTTLYTLPENKNLEFLNYSKDITPPFVIYADFESVLPPDDRHHQIHKPIAAGLLLINNYTNT 314

HSP 2 Score: 154.066 bits (388), Expect = 0.000e+0
Identity = 78/128 (60.94%), Postives = 99/128 (77.34%), Query Frame = 1 
Query: 1525 FYGIPGMSW---VMSLTEPIELLQDMKMFDFFEGEMLGELTYVNKHYAATSEYIELLYIDINNVYGWALSQNLPFSDFKWVHYRMNDVLQESMN-EEIELLPHGNTMEVDIDIPDSLH----NFPLAP 1884
            FYGIPGMSW   +M+LTEPIELLQDM+MF+F+EG + G LT+VNKHY A  E  ELLY DINN+YGWALSQ+LP++DF WV+  +++ L E  N  +IE+LP+G  MEVDI+IPD LH    +FP+ P
Sbjct:  547 FYGIPGMSWASALMTLTEPIELLQDMEMFNFYEGGIRGGLTFVNKHYIAALEDTELLYKDINNLYGWALSQSLPYADFTWVYDCLDEFLSECANVVDIEILPYGYIMEVDIEIPDYLHDFLNDFPIEP 674

HSP 3 Score: 120.553 bits (301), Expect = 0.000e+0
Identity = 65/144 (45.14%), Postives = 74/144 (51.39%), Query Frame = 3 
Query: 1254 DMYHILKYGIDEFPYWELNIIPTSTK-----------------------------------------------------LPPIWSNVSEDEYAKAQLIWNQQNCNTLLDNMLAYLKLDVFILAKYFQQFSTKAIAYNGIEHLGF 1526
            D++HILKYG+D FP W L+IIPTST+                                                     LPPIW  VS+DEY +AQLIW QQNC TL D ML YLKLDVF+LA YFQQF  KAI YNG+E L F
Sbjct:  404 DLHHILKYGLDRFPSWNLDIIPTSTEKFISLIVHINKLAVRFIDSMQFVNCSLAKAVKTLTSLPLTSSVYQGSIVDCKELPPIWEGVSKDEYDRAQLIWTQQNCRTLRDYMLIYLKLDVFLLADYFQQFRAKAIVYNGLEPLNF 547

HSP 4 Score: 102.449 bits (254), Expect = 0.000e+0
Identity = 95/234 (40.60%), Postives = 128/234 (54.70%), Query Frame = 2 
Query: 1874 HLLRKNYVXXXXXXXXXXXXGEKVVNIHITIRFKQSPIFKKYLDTNINLRASAVSELCRDLYKLYNNSLYGKTVENLKMRMNLWLLNNSTETLITYSSKLSFRKTIKIDENLFAAHLNKLH*TVC---TGFIQTTNVSTPTH*IGEI*ETI*LPK---------NIVTGDIDSFFGELE-------FKARFSFASVDLLDTPNYDPSHPLYYRNL*SESLKMRAKELDTLNGFS 2518
            H  +KNYVLH  LL+LYLSLG KV  IH   ++KQ+PIF+ Y+D N NLRA+A                          RMNL L N S E LITYSSK +FRK+I+I ++LFAAHLNK   ++C     +I  + +      + ++ + + L K         N+V GD  SFF E++        KA+    S DLLDT NYDPSHPLY R L  +S+  + K+   + G+S
Sbjct:  691 HYPKKNYVLHWRLLRLYLSLGAKVTYIHRANKYKQAPIFQNYMDKNTNLRANA--------------------------RMNLRLCN-SAERLITYSSKPAFRKSIEITDDLFAAHLNKD--SICLDRPSYISQSVLDLSKLWMYQL-QYVELQKYRDIYNCDINLVAGDTYSFFLEIKNFKLDILLKAKI---SDDLLDTSNYDPSHPLYSRRL--DSVIGKFKDESKVLGYS 889

HSP 5 Score: 55.8398 bits (133), Expect = 0.000e+0
Identity = 28/55 (50.91%), Postives = 35/55 (63.64%), Query Frame = 2 
Query: 1109 TCHICKKPI-----NTC*ILR*FLW*ISGPACRKFNLSRRIQRQLSVVFHNLRGY 1258
            TC++CKKPI     + C     +L    G ACRK N++RRI R L +VFHNLRGY
Sbjct:  353 TCYLCKKPIVHPVKDHCHFTGNYL----GAACRKCNIARRIYRLLPIVFHNLRGY 403

HSP 6 Score: 52.7582 bits (125), Expect = 0.000e+0
Identity = 24/41 (58.54%), Postives = 29/41 (70.73%), Query Frame = 1 
Query:  937 TQKQQYFEFYEPDCIPLFLKKVEEIAVTIVLPYYEIEGKIL 1059
            T  + Y+ FY  DCIP FLKK+EEIA  IV+PYY+ EG  L
Sbjct:  312 TNTESYYYFYGLDCIPDFLKKIEEIATEIVIPYYDSEGDKL 352

HSP 7 Score: 114.775 bits (286), Expect = 1.738e-25
Identity = 55/85 (64.71%), Postives = 66/85 (77.65%), Query Frame = 1 
Query: 2461 VIGKFKDES*GVGYTEWVFLCPKCYHLLGNKSSMKAKGVTLPGKHIKH---I*MFTTIAQLSVPQTRIGTRNHHLYTFKNSKIMM 2706
            VIGKFKDES  +GY+EW F+ PKCY LLGNKS+M+AKGV LPG  IKH   +  +     +SVPQTRIGTRNH L+TFKNSKI++
Sbjct:  875 VIGKFKDESKVLGYSEWEFVRPKCYSLLGNKSTMRAKGVKLPGTDIKHQSYLHCYNNNTIMSVPQTRIGTRNHKLFTFKNSKIVL 959

HSP 8 Score: 78.5666 bits (192), Expect = 2.218e-14
Identity = 69/205 (33.66%), Postives = 97/205 (47.32%), Query Frame = 3 
Query: 2229 SYIRQSVLDLSKQRMYQLQHIELEKYRKLFNCQ-KXXXXXXXXXFLEN*NSKLDFHLPALIC---WTXXXXXXXXXXXXGTCDRKV*R*ELRSWIH*MG-----FLVSKMLSSVRKQIIDESQGCNI--TW*TYKTYLDVYNNSTVVCT-----SD*NRNXXXXXXXXXXXXNHDDKRAWVGKYTSFPYGDYISAYVIDIDNNIT 2795
            SYI QSVLDLSK  MYQLQ++EL+KYR ++NC   +        FLE  N KLD  L A I                     D  + + +  S +  +G     F+  K  S +  +    ++G  +  T   +++YL  YNN+T++          N    +       L NHDDKRAWVGK +SF YG Y    V+D+ +NI 
Sbjct:  791 SYISQSVLDLSKLWMYQLQYVELQKYRDIYNCDINLVAGDTYSFFLEIKNFKLDILLKAKISDDLLDTSNYDPSHPLYSRRLDSVIGKFKDESKV--LGYSEWEFVRPKCYSLLGNKSTMRAKGVKLPGTDIKHQSYLHCYNNNTIMSVPQTRIGTRNHKLFTFKNSKIVLNNHDDKRAWVGKNSSFAYGHYRGVDVLDVGSNIV 993
[Close]
BLAST of Rho_N domain-containing protein vs. Planmine SMEST
Match: SMESG000040848.1 (SMESG000040848.1)

HSP 1 Score: 258.455 bits (659), Expect = 1.675e-155
Identity = 130/196 (66.33%), Postives = 151/196 (77.04%), Query Frame = 3 
Query:  396 LEYDEIT-------KYLQI*KENASLNLAINVLFYTPIVIIDEVPDSYVFHHPN-----RSKVTDETPIYLCLLEKSDRNHYTAVSDLNKLTNCHQNPDCPVQIRCEWCPRCLNSFRLDNAFNKHVSLCAKNQMGTTLYTLSDNKDLKFENYSKTITPPYVIYADFESVLPHDVRHHQIHMPSSAGLLLLNNYTKT 947
            LEYDEIT          ++ K+N++L LAINV  YTP VIID+VPD+YVFHH +     RSKVTD  P YL LLE +DR HYTAV+++ +L NCHQ PDC +QIR  WCP CLN FRLDNAFN HV+LCAKNQ GTTLYTL +NK+L+F NYSK ITPP+VIYADFESVLP D RHHQIH P +AGLLL+NNYT T
Sbjct:  123 LEYDEITDCPMKLINIPKVEKKNSTLKLAINVSSYTPTVIIDKVPDNYVFHHSHLSLIHRSKVTDGNPFYLLLLENNDRYHYTAVTNIERLMNCHQTPDCNIQIRYRWCPHCLNGFRLDNAFNTHVALCAKNQTGTTLYTLPENKNLEFLNYSKDITPPFVIYADFESVLPPDDRHHQIHKPIAAGLLLINNYTNT 318

HSP 2 Score: 154.066 bits (388), Expect = 1.675e-155
Identity = 78/128 (60.94%), Postives = 99/128 (77.34%), Query Frame = 1 
Query: 1525 FYGIPGMSW---VMSLTEPIELLQDMKMFDFFEGEMLGELTYVNKHYAATSEYIELLYIDINNVYGWALSQNLPFSDFKWVHYRMNDVLQESMN-EEIELLPHGNTMEVDIDIPDSLH----NFPLAP 1884
            FYGIPGMSW   +M+LTEPIELLQDM+MF+F+EG + G LT+VNKHY A  E  ELLY DINN+YGWALSQ+LP++DF WV+  +++ L E  N  +IE+LP+G  MEVDI+IPD LH    +FP+ P
Sbjct:  551 FYGIPGMSWASALMTLTEPIELLQDMEMFNFYEGGIRGGLTFVNKHYIAALEDTELLYKDINNLYGWALSQSLPYADFTWVYDCLDEFLSECANVVDIEILPYGYIMEVDIEIPDYLHDFLNDFPIEP 678

HSP 3 Score: 120.553 bits (301), Expect = 1.675e-155
Identity = 65/144 (45.14%), Postives = 74/144 (51.39%), Query Frame = 3 
Query: 1254 DMYHILKYGIDEFPYWELNIIPTSTK-----------------------------------------------------LPPIWSNVSEDEYAKAQLIWNQQNCNTLLDNMLAYLKLDVFILAKYFQQFSTKAIAYNGIEHLGF 1526
            D++HILKYG+D FP W L+IIPTST+                                                     LPPIW  VS+DEY +AQLIW QQNC TL D ML YLKLDVF+LA YFQQF  KAI YNG+E L F
Sbjct:  408 DLHHILKYGLDRFPSWNLDIIPTSTEKFISLIVHINKLAVRFIDSMQFVNCSLAKAVKTLTSLPLTSSVYQGSIVDCKELPPIWEGVSKDEYDRAQLIWTQQNCRTLRDYMLIYLKLDVFLLADYFQQFRAKAIVYNGLEPLNF 551

HSP 4 Score: 52.373 bits (124), Expect = 1.675e-155
Identity = 24/41 (58.54%), Postives = 29/41 (70.73%), Query Frame = 1 
Query:  937 TQKQQYFEFYEPDCIPLFLKKVEEIAVTIVLPYYEIEGKIL 1059
            T  + Y+ FY  DCIP FLKK+EEIA  IV+PYY+ EG  L
Sbjct:  316 TNTESYYYFYGLDCIPDFLKKIEEIATEIVIPYYDSEGDKL 356

HSP 5 Score: 51.2174 bits (121), Expect = 1.675e-155
Identity = 28/55 (50.91%), Postives = 35/55 (63.64%), Query Frame = 2 
Query: 1109 TCHICKKPI-----NTC*ILR*FLW*ISGPACRKFNLSRRIQRQLSVVFHNLRGY 1258
            TC++CKKPI     + C     +L    G ACRK N++RRI R L +VFHNLRGY
Sbjct:  357 TCYLCKKPIVHPVKDHCHFTGNYL----GAACRKCNIARRIYRLLPIVFHNLRGY 407

HSP 6 Score: 115.161 bits (287), Expect = 1.516e-25
Identity = 55/85 (64.71%), Postives = 66/85 (77.65%), Query Frame = 1 
Query: 2461 VIGKFKDES*GVGYTEWVFLCPKCYHLLGNKSSMKAKGVTLPGKHIKH---I*MFTTIAQLSVPQTRIGTRNHHLYTFKNSKIMM 2706
            VIGKFKDES  +GY+EW F+ PKCY LLGNKS+M+AKGV LPG  IKH   +  +     +SVPQTRIGTRNH L+TFKNSKI++
Sbjct:  849 VIGKFKDESKVLGYSEWEFVRPKCYSLLGNKSTMRAKGVKLPGTDIKHQSYLHCYNNNTIMSVPQTRIGTRNHKLFTFKNSKIVL 933

HSP 7 Score: 78.9518 bits (193), Expect = 1.756e-14
Identity = 69/205 (33.66%), Postives = 97/205 (47.32%), Query Frame = 3 
Query: 2229 SYIRQSVLDLSKQRMYQLQHIELEKYRKLFNCQ-KXXXXXXXXXFLEN*NSKLDFHLPALIC---WTXXXXXXXXXXXXGTCDRKV*R*ELRSWIH*MG-----FLVSKMLSSVRKQIIDESQGCNI--TW*TYKTYLDVYNNSTVVCT-----SD*NRNXXXXXXXXXXXXNHDDKRAWVGKYTSFPYGDYISAYVIDIDNNIT 2795
            SYI QSVLDLSK  MYQLQ++EL+KYR ++NC   +        FLE  N KLD  L A I                     D  + + +  S +  +G     F+  K  S +  +    ++G  +  T   +++YL  YNN+T++          N    +       L NHDDKRAWVGK +SF YG Y    V+D+ +NI 
Sbjct:  765 SYISQSVLDLSKLWMYQLQYVELQKYRDIYNCDINLVAGDTYSFFLEIKNFKLDILLKAKISDDLLDTSNYDPSHPLYSRRLDSVIGKFKDESKV--LGYSEWEFVRPKCYSLLGNKSTMRAKGVKLPGTDIKHQSYLHCYNNNTIMSVPQTRIGTRNHKLFTFKNSKIVLNNHDDKRAWVGKNSSFAYGHYRGVDVLDVGSNIV 967

HSP 8 Score: 73.1738 bits (178), Expect = 1.053e-12
Identity = 63/155 (40.65%), Postives = 88/155 (56.77%), Query Frame = 2 
Query: 2111 RMNLWLLNNSTETLITYSSKLSFRKTIKIDENLFAAHLNKLH*TVC---TGFIQTTNVSTPTH*IGEI*ETI*LPK---------NIVTGDIDSFFGELE-------FKARFSFASVDLLDTPNYDPSHPLYYRNL*SESLKMRAKELDTLNGFS 2518
            RMNL L N S E LITYSSK +FRK+I+I ++LFAAHLNK   ++C     +I  + +      + ++ + + L K         N+V GD  SFF E++        KA+    S DLLDT NYDPSHPLY R L  +S+  + K+   + G+S
Sbjct:  718 RMNLRLCN-SAERLITYSSKPAFRKSIEITDDLFAAHLNKD--SICLDRPSYISQSVLDLSKLWMYQL-QYVELQKYRDIYNCDINLVAGDTYSFFLEIKNFKLDILLKAKI---SDDLLDTSNYDPSHPLYSRRL--DSVIGKFKDESKVLGYS 863
[Close]
BLAST of Rho_N domain-containing protein vs. Planmine SMEST
Match: SMESG000061056.1 (SMESG000061056.1)

HSP 1 Score: 315.464 bits (807), Expect = 2.114e-139
Identity = 159/196 (81.12%), Postives = 167/196 (85.20%), Query Frame = 3 
Query:  396 LEYDEIT----KYLQI*K---ENASLNLAINVLFYTPIVIIDEVPDSYVFHHPN-----RSKVTDETPIYLCLLEKSDRNHYTAVSDLNKLTNCHQNPDCPVQIRCEWCPRCLNSFRLDNAFNKHVSLCAKNQMGTTLYTLSDNKDLKFENYSKTITPPYVIYADFESVLPHDVRHHQIHMPSSAGLLLLNNYTKT 947
            L YDEIT    K + I K   +N SLNLAINVL YTP VIIDEVPDSYVFHHP+     RSKV   TPIYL LLEKSDRNHYTAVSDLNKL NCHQNPDCP+QIRC+WCP CLN FRLDNAFNKHV+LCAKNQ GTTLYTL DNK+LKFENYSKTITPPYVIYADFESVLP DVRHHQIHMPSSAGLLL+NNYTKT
Sbjct:  960 LVYDEITDCPMKLINIPKFEKKNDSLNLAINVLSYTPTVIIDEVPDSYVFHHPHLSIIHRSKVIGGTPIYLLLLEKSDRNHYTAVSDLNKLMNCHQNPDCPIQIRCKWCPHCLNGFRLDNAFNKHVALCAKNQTGTTLYTLPDNKNLKFENYSKTITPPYVIYADFESVLPPDVRHHQIHMPSSAGLLLINNYTKT 1155

HSP 2 Score: 119.783 bits (299), Expect = 2.114e-139
Identity = 58/65 (89.23%), Postives = 60/65 (92.31%), Query Frame = 1 
Query:  202 KKVHLGRGWRVINILEIDFILTKFSPISRLSGHRFIQTPSAIISKKAIINVKNTNDKCVLYSILA 396
            K  HLG GWRVINILEIDFILTKFSPISRLSGHR+IQTPSAI SKKAIINVKNT+DKC LYSILA
Sbjct:  872 KFTHLGSGWRVINILEIDFILTKFSPISRLSGHRYIQTPSAIASKKAIINVKNTDDKCFLYSILA 936

HSP 3 Score: 77.0258 bits (188), Expect = 2.114e-139
Identity = 35/49 (71.43%), Postives = 38/49 (77.55%), Query Frame = 1 
Query:  937 TQKQQYFEFYEPDCIPLFLKKVEEIAVTIVLPYYEIEGKILFEIHDFCR 1083
            T+ QQYFEFY PDCI LFLKKVEEIAVTIVLPYY++EGK L     F  
Sbjct: 1153 TKTQQYFEFYGPDCISLFLKKVEEIAVTIVLPYYDVEGKKLVSFMSFAE 1201

HSP 4 Score: 41.5874 bits (96), Expect = 2.114e-139
Identity = 14/22 (63.64%), Postives = 20/22 (90.91%), Query Frame = 2 
Query: 1070 MTFAEELQFQSCSTCHICKKPI 1135
            M+FAE+ +F+SC TC++CKKPI
Sbjct: 1197 MSFAEQREFKSCRTCYLCKKPI 1218

HSP 5 Score: 28.8758 bits (63), Expect = 2.114e-139
Identity = 13/18 (72.22%), Postives = 14/18 (77.78%), Query Frame = 2 
Query:  176 GVNKLNEKIKKFI*AGDG 229
            GVNKLNEKI+KF   G G
Sbjct:  862 GVNKLNEKIEKFTHLGSG 879

HSP 6 Score: 167.162 bits (422), Expect = 5.516e-91
Identity = 116/215 (53.95%), Postives = 143/215 (66.51%), Query Frame = 2 
Query: 1874 HLLRKNYVXXXXXXXXXXXXGEKVVNIHITIRFKQSPIFKKYLDTNINLRASAVSELCRDLYKLYNNSLYGKTVENLKMRMNLWLLNNSTETLITYSSKLSFRKTIKIDENLFAAHLNKLH*TVC---TGFIQTTNVSTPTH*IGEI*ETI*LPK---------NIVTGDIDSFFGELE-------FKARFSFASVDLLDTPNYDPSHPLYYRNL 2461
            H  +KN+VLH  LL+LYLSLG KVVN+H  I+F Q PIFK Y+D N +LRA+AVSE+ +DL+KLYNNSLYGK+VENLK RMNL L  NS E LITYSSK  FRKTI++DENLFAAHLNK   ++C     +I  + +      + ++ + I L K         N++ GD DSFF E++        KA     S DLLDT NYDPSHPLY R L
Sbjct: 1443 HYPKKNHVLHWRLLRLYLSLGAKVVNVHRCIKFTQKPIFKNYIDKNTDLRANAVSEIYKDLFKLYNNSLYGKSVENLKKRMNLRLC-NSFEKLITYSSKPEFRKTIEVDENLFAAHLNKD--SICLDRPSYIGQSVLDLSKLRMYQL-QYIELQKYRTIYNCDINLIAGDTDSFFLEIKNLKLDTLLKA---MISDDLLDTSNYDPSHPLYSRRL 1650

HSP 7 Score: 108.227 bits (269), Expect = 5.516e-91
Identity = 54/85 (63.53%), Postives = 64/85 (75.29%), Query Frame = 1 
Query: 2461 VIGKFKDES*GVGYTEWVFLCPKCYHLLGNKSSMKAKGVTLPGKHIKH---I*MFTTIAQLSVPQTRIGTRNHHLYTFKNSKIMM 2706
            VIGKFKDES G  Y+EWVFL PKCY LLGNKS+M+AKGV L G +I H   +  +     +SVPQTRIG+RNH L+TFKNSKI +
Sbjct: 1653 VIGKFKDESKGGVYSEWVFLRPKCYSLLGNKSTMRAKGVKLAGTNINHQSYLDCYNNNEIISVPQTRIGSRNHQLFTFKNSKIAL 1737

HSP 8 Score: 102.449 bits (254), Expect = 5.516e-91
Identity = 47/77 (61.04%), Postives = 61/77 (79.22%), Query Frame = 1 
Query: 1669 EYIELLYIDINNVYGWALSQNLPFSDFKWVHYRMNDVLQESMN-EEIELLPHGNTMEVDIDIPDSLH----NFPLAP 1884
            E  ELLYIDINN+YGWALSQ+LP++DF WV+  ++DVL E MN  +IE+LP+G TMEVDIDIP++ H    +FP+ P
Sbjct: 1350 EDTELLYIDINNLYGWALSQSLPYADFVWVYDGLDDVLNECMNVADIEILPYGYTMEVDIDIPENFHDFLNDFPIGP 1426

HSP 9 Score: 99.7525 bits (247), Expect = 1.140e-20
Identity = 45/86 (52.33%), Postives = 60/86 (69.77%), Query Frame = 3 
Query: 1290 FPY---WELNIIPTSTKLPPIWSNVSEDEYAKAQLIWNQQNCNTLLDNMLAYLKLDVFILAKYFQQFSTKAIAYNGIEHLGFLRNS 1538
            FPY      +++ T+ +LPP W  V++DEYA A+L+W QQNC  L D M+ YLK+DVF+LA YFQQF  K IAYNG+E L F  ++
Sbjct: 1267 FPYDYAASFDVLETTYELPPKWEGVTDDEYATAELVWMQQNCGNLRDYMMHYLKVDVFLLADYFQQFREKTIAYNGLEPLNFYEDT 1352

HSP 10 Score: 78.1814 bits (191), Expect = 3.661e-14
Identity = 70/207 (33.82%), Postives = 97/207 (46.86%), Query Frame = 3 
Query: 2229 SYIRQSVLDLSKQRMYQLQHIELEKYRKLFNCQ-KXXXXXXXXXFLEN*NSKLDFHLPALIC----------WTXXXXXXXXXXXXGTCDRKV*R*ELRSWIH*MGFLVSKMLSSVRKQIIDESQGCNI--TW*TYKTYLDVYNNSTVVCT-----SD*NRNXXXXXXXXXXXXNHDDKRAWVGKYTSFPYGDYISAYVIDIDNNIT 2795
            SYI QSVLDLSK RMYQLQ+IEL+KYR ++NC   +        FLE  N KLD  L A+I            +  +    +  +IG    +        W+    FL  K  S +  +    ++G  +  T   +++YLD YNN+ ++          N    +       L NHDDKRAWV K  SF YG Y     I+  ++IT
Sbjct: 1569 SYIGQSVLDLSKLRMYQLQYIELQKYRTIYNCDINLIAGDTDSFFLEIKNLKLDTLLKAMISDDLLDTSNYDPSHPLYSRRLASVIGKFKDESKGGVYSEWV----FLRPKCYSLLGNKSTMRAKGVKLAGTNINHQSYLDCYNNNEIISVPQTRIGSRNHQLFTFKNSKIALNNHDDKRAWVDKNVSFAYGHYRGLDAINSGHSIT 1771
[Close]
BLAST of Rho_N domain-containing protein vs. Planmine SMEST
Match: SMESG000076702.1 (SMESG000076702.1)

HSP 1 Score: 234.572 bits (597), Expect = 6.499e-137
Identity = 152/399 (38.10%), Postives = 205/399 (51.38%), Query Frame = 3 
Query:  585 KSDRNHYTAVSDLNKLTNCHQNPDCPVQIRCEWCPRCLNSFRLDNAFNKHVSLCAKNQMGTTLYTLSDNKD--LKFENYSKTITPPYVIYADFESVLPHDVRHHQIHMPSSAGLLLLNNYTKTAIF*IL*T*LHSFIS*KSRGNCCHNCPSIL*N*R*NFV*NP*LLQRNCNFNLVVLVTY---------------VKNLLIHVRYYDNFSGKYLGQPVENLICLAEFKD-------NYLSYFII*EDMYHILKYGIDEFPYWELNIIPTST-----------------------------------------------------KLPPIWSNVSEDEYAKAQLIWNQQNCNTLLDNMLAYLKLDVFILAKYFQQFSTKAIAYNGIE---HLGFLRNSW 1541
            + ++ HYTAV++L  L NCH+N DC V+I C+WC  CLN FR   +F KH  LC KNQ+GTTLYTL  +KD  L+F+N+SKTITPP+VIYADFES+LP D  + Q H+P SAGLLL+NN+T    +       H FI      NC +     + +   N V  P    +     L++ +T+                KN++  VR +D+F+GKY+G   ++       K+       N   Y     D++HILKYGI++FP W L+ IP S+                                                     +LPPIW  VSE EY KAQLIW +  C  LLD M+ YLKLD F+LA +FQQF  K+IA+N +E   + G    SW
Sbjct:  370 RGEKYHYTAVTNLQSLMNCHKNNDCNVRICCKWCENCLNGFRKPKSFEKHQELCKKNQLGTTLYTLPKDKDKYLEFKNWSKTITPPFVIYADFESILPKDDIYFQKHLPISAGLLLINNFTGMTSY-------HHFIG----QNCVYEFLKKVSDIGTNIV-LP--YYKENGKKLMLPLTFDEEYSFDNCEYCYLCNKNIVDKVRDHDHFTGKYIGPACKSCNVNRSIKELLPVVFHNLRGY-----DLHHILKYGINQFPNWNLSCIPISSEKFLSLMVYVGKLRIRFIDSYQFANDSLARAVKTLTSLPLTKSVFTEVLENTYELPPIWEGVSEVEYEKAQLIWTETKCVNLLDYMMIYLKLDCFLLADFFQQFRAKSIAHNSLEPLNYFGIPGMSW 749

HSP 2 Score: 130.568 bits (327), Expect = 6.499e-137
Identity = 62/126 (49.21%), Postives = 92/126 (73.02%), Query Frame = 1 
Query: 1525 FYGIPGMSW---VMSLTEPIELLQDMKMFDFFEGEMLGELTYVNKHYAATSEYIELLYIDINNVYGWALSQNLPFSDFKWVHYRMNDVLQESMNEEIELLPHGNTMEVDIDIPDSLH----NFPLA 1881
            ++GIPGMSW   +++L +PIELLQDM++++F+EG + G LT+VNKH+  T +  ELLYIDINN+YGWALSQ LP S+F W+    + +L + +   +E L +G T+EV+++IP  LH    +FP+A
Sbjct:  741 YFGIPGMSWSSALITLDKPIELLQDMEIYNFYEGGIRGCLTFVNKHFVKTDKITELLYIDINNLYGWALSQPLPCSEFNWIFDGFDTILFDCLTLNLESLSYGYTLEVNLEIPSDLHDKLDDFPIA 866

HSP 3 Score: 95.5153 bits (236), Expect = 6.499e-137
Identity = 48/88 (54.55%), Postives = 56/88 (63.64%), Query Frame = 1 
Query: 2461 VIGKFKDES*GVGYTEWVFLCPKCYHLLGNKSSMKAKGVTLPGKHIKH---I*MFTTIAQLSVPQTRIGTRNHHLYTFKNSKIMMIRE 2715
            VIGKFKDES G GY EWVFL PKCY LLG    +KAKGVTL    + H      +   +  SVPQ RIGT NH LY+FK SK+ +  +
Sbjct: 1076 VIGKFKDESKGEGYDEWVFLRPKCYSLLGKSKCLKAKGVTLKDTTLTHKSYKDCYEKNSTFSVPQFRIGTSNHQLYSFKTSKVALTNK 1163

HSP 4 Score: 92.0485 bits (227), Expect = 6.499e-137
Identity = 85/222 (38.29%), Postives = 118/222 (53.15%), Query Frame = 2 
Query: 1874 HLLRKNYVXXXXXXXXXXXXGEKVVNIHITIRFKQSPIFKKYLDTNINLRASAVSELCRDLYKLYNNSLYGKTVENLKMRMNLWLLNNSTETLITYSSKLSFRKTIKIDENLFAAHLNKLH*TVCTGFIQTTNVSTPTH*IGEI*ETI*LPK----------------------NIVTGDIDSFFGELE-FKARFSFASV---DLLDTPNYDPSHPLYYRNL 2461
            H  +KNYV+H  LLKLYLSLG +V +++  +RFKQSPIFK Y+DTN  LRA + + L ++ YKL NNSLY K                    +ITY+SK +F+++IKI ++L AA LN    +VC        +  P++ IG+    + L K                      NIV GD DSFF E++  K     + +   +LLDT NY  +HPLY + L
Sbjct:  884 HYPKKNYVIHWRLLKLYLSLGVEVTHVNRAVRFKQSPIFKNYIDTNTELRAKSTNTLDKNYYKLLNNSLYEK-------------------KMITYASKPTFQRSIKIADDLIAALLNPD--SVC--------LDRPSY-IGQ--SVLDLSKLRMYELQYKDLEKYRRQFNCEINIVAGDTDSFFLEIKNCKLDILLSEMVKDNLLDTSNYPSTHPLYSKKL 1073

HSP 5 Score: 70.8626 bits (172), Expect = 6.342e-15
Identity = 32/64 (50.00%), Postives = 46/64 (71.88%), Query Frame = 1 
Query:  205 KVHLGRGWRVINILEIDFILTKFSPISRLSGHRFIQTPSAIISKKAIINVKNTNDKCVLYSILA 396
            +  LG  W++  I+E++F +TK S ISRLSGH +IQTP A++ KKAI+NV+NT+      S+LA
Sbjct:  288 RTELGSSWKINRIIEVNFKVTKVSNISRLSGHSYIQTPKALMGKKAIVNVQNTDKFMFHISVLA 351

HSP 6 Score: 30.4166 bits (67), Expect = 6.342e-15
Identity = 22/55 (40.00%), Postives = 29/55 (52.73%), Query Frame = 2 
Query:   50 IKIMLTERISSKLI----NEIGKMGEILDNSFSNYTEKFSDSFLSEGVNKLNEKI 202
            +KI L E   SK+      E  K GE+   S SN     + +FL EG+ K+NEKI
Sbjct:  231 LKITLNEMKHSKVQFGVQVEFEKDGELKVVSISNRAASVTTTFLDEGMKKINEKI 285

HSP 7 Score: 55.0694 bits (131), Expect = 1.944e-12
Identity = 31/82 (37.80%), Postives = 42/82 (51.22%), Query Frame = 2 
Query: 1067 SMTFAEELQFQSCSTCHICKKPI-NTC*ILR*FLW*ISGPACRKFNLSRRIQRQLSVVFHNLRGY---------VSYFKIWN 1282
             +TF EE  F +C  C++C K I +       F     GPAC+  N++R I+  L VVFHNLRGY         ++ F  WN
Sbjct:  533 PLTFDEEYSFDNCEYCYLCNKNIVDKVRDHDHFTGKYIGPACKSCNVNRSIKELLPVVFHNLRGYDLHHILKYGINQFPNWN 614

HSP 8 Score: 38.1206 bits (87), Expect = 1.944e-12
Identity = 18/36 (50.00%), Postives = 22/36 (61.11%), Query Frame = 1 
Query:  952 YFEFYEPDCIPLFLKKVEEIAVTIVLPYYEIEGKIL 1059
            Y  F   +C+  FLKKV +I   IVLPYY+  GK L
Sbjct:  495 YHHFIGQNCVYEFLKKVSDIGTNIVLPYYKENGKKL 530

HSP 9 Score: 62.7734 bits (151), Expect = 1.593e-9
Identity = 71/206 (34.47%), Postives = 94/206 (45.63%), Query Frame = 3 
Query: 2229 SYIRQSVLDLSKQRMYQLQHIELEKYRKLFNCQ-KXXXXXXXXXFLEN*NSKLDFHLPALIC----------WTXXXXXXXXXXXXGTCDRKV*R*ELRSWIH*MGFLVSKMLSSVRKQIIDESQGCNI--TW*TYKTYLDVY-NNSTV------VCTSD*NRNXXXXXXXXXXXXNHDDKRAWVGKYTSFPYGDYISAYVIDIDN 2786
            SYI QSVLDLSK RMY+LQ+ +LEKYR+ FNC+  I        FLE  N KLD  L  ++            T  +    +  +IG    +        W+    FL  K  S + K    +++G  +  T  T+K+Y D Y  NST       + TS  N    S       L N +DKR WV K  S  YG Y+    I+ DN
Sbjct:  992 SYIGQSVLDLSKLRMYELQYKDLEKYRRQFNCEINIVAGDTDSFFLEIKNCKLDILLSEMVKDNLLDTSNYPSTHPLYSKKLDSVIGKFKDESKGEGYDEWV----FLRPKCYSLLGKSKCLKAKGVTLKDTTLTHKSYKDCYEKNSTFSVPQFRIGTS--NHQLYSFKTSKVALTNKEDKRVWVEKNVSLAYGHYM----IETDN 1187
[Close]
BLAST of Rho_N domain-containing protein vs. Planmine SMEST
Match: SMESG000076702.1 (SMESG000076702.1)

HSP 1 Score: 248.054 bits (632), Expect = 5.544e-134
Identity = 152/363 (41.87%), Postives = 205/363 (56.47%), Query Frame = 3 
Query:  585 KSDRNHYTAVSDLNKLTNCHQNPDCPVQIRCEWCPRCLNSFRLDNAFNKHVSLCAKNQMGTTLYTLSDNKD--LKFENYSKTITPPYVIYADFESVLPHDVRHHQIHMPSSAGLLLLNNYTKTAIF*IL*T*LHSFIS*KSRGNCCHNCPSIL*N*R*NFV*NP*LLQRNCNFNLVVLVTY---------------VKNLLIHVRYYDNFSGKYLGQPVENLICLAEFKD-------NYLSYFII*EDMYHILKYGIDEFPYWELNIIPTST-----------------KLPPIWSNVSEDEYAKAQLIWNQQNCNTLLDNMLAYLKLDVFILAKYFQQFSTKAIAYNGIE---HLGFLRNSW 1541
            + ++ HYTAV++L  L NCH+N DC V+I C+WC  CLN FR   +F KH  LC KNQ+GTTLYTL  +KD  L+F+N+SKTITPP+VIYADFES+LP D  + Q H+P SAGLLL+NN+T    +       H FI      NC +     + +   N V  P    +     L++ +T+                KN++  VR +D+F+GKY+G   ++       K+       N   Y     D++HILKYGI++FP W L+ IP S+                 +LPPIW  VSE EY KAQLIW +  C  LLD M+ YLKLD F+LA +FQQF  K+IA+N +E   + G    SW
Sbjct:  370 RGEKYHYTAVTNLQSLMNCHKNNDCNVRICCKWCENCLNGFRKPKSFEKHQELCKKNQLGTTLYTLPKDKDKYLEFKNWSKTITPPFVIYADFESILPKDDIYFQKHLPISAGLLLINNFTGMTSY-------HHFIG----QNCVYEFLKKVSDIGTNIV-LP--YYKENGKKLMLPLTFDEEYSFDNCEYCYLCNKNIVDKVRDHDHFTGKYIGPACKSCNVNRSIKELLPVVFHNLRGY-----DLHHILKYGINQFPNWNLSCIPISSEKFLSLMVYVEVLENTYELPPIWEGVSEVEYEKAQLIWTETKCVNLLDYMMIYLKLDCFLLADFFQQFRAKSIAHNSLEPLNYFGIPGMSW 713

HSP 2 Score: 130.568 bits (327), Expect = 5.544e-134
Identity = 62/126 (49.21%), Postives = 92/126 (73.02%), Query Frame = 1 
Query: 1525 FYGIPGMSW---VMSLTEPIELLQDMKMFDFFEGEMLGELTYVNKHYAATSEYIELLYIDINNVYGWALSQNLPFSDFKWVHYRMNDVLQESMNEEIELLPHGNTMEVDIDIPDSLH----NFPLA 1881
            ++GIPGMSW   +++L +PIELLQDM++++F+EG + G LT+VNKH+  T +  ELLYIDINN+YGWALSQ LP S+F W+    + +L + +   +E L +G T+EV+++IP  LH    +FP+A
Sbjct:  705 YFGIPGMSWSSALITLDKPIELLQDMEIYNFYEGGIRGCLTFVNKHFVKTDKITELLYIDINNLYGWALSQPLPCSEFNWIFDGFDTILFDCLTLNLESLSYGYTLEVNLEIPSDLHDKLDDFPIA 830

HSP 3 Score: 95.5153 bits (236), Expect = 5.544e-134
Identity = 48/88 (54.55%), Postives = 56/88 (63.64%), Query Frame = 1 
Query: 2461 VIGKFKDES*GVGYTEWVFLCPKCYHLLGNKSSMKAKGVTLPGKHIKH---I*MFTTIAQLSVPQTRIGTRNHHLYTFKNSKIMMIRE 2715
            VIGKFKDES G GY EWVFL PKCY LLG    +KAKGVTL    + H      +   +  SVPQ RIGT NH LY+FK SK+ +  +
Sbjct: 1022 VIGKFKDESKGEGYDEWVFLRPKCYSLLGKSKCLKAKGVTLKDTTLTHKSYKDCYEKNSTFSVPQFRIGTSNHQLYSFKTSKVALTNK 1109

HSP 4 Score: 69.3218 bits (168), Expect = 5.544e-134
Identity = 74/212 (34.91%), Postives = 105/212 (49.53%), Query Frame = 2 
Query: 1874 HLLRKNYVXXXXXXXXXXXXGEKVVNIHITIRFKQSPIFKKYLDTNINLRASAVSELCRDLYKLYNNSLYGKTVENLKMRMNLWLLNNSTETLITYSSKLSFRKTIKIDENLFAAHLNKLH*TVC---TGFIQTTNVSTPTH*IGEI*ETI*LPK---------NIVTGDIDSFFGELEF-KARFSFASV---DLLDTPNYDPSHPLYYRNL 2461
            H  +KNYV+H  LLKLYLSLG +V +++  +RFKQSPIFK Y+DTN  LRA                                       + +ITY+SK +F+++IKI ++L AA LN    +VC     +I  + +      + E+ +   L K         NIV GD DSFF E++  K     + +   +LLDT NY  +HPLY + L
Sbjct:  848 HYPKKNYVIHWRLLKLYLSLGVEVTHVNRAVRFKQSPIFKNYIDTNTELRAKK-------------------------------------KKMITYASKPTFQRSIKIADDLIAALLNPD--SVCLDRPSYIGQSVLDLSKLRMYEL-QYKDLEKYRRQFNCEINIVAGDTDSFFLEIKNCKLDILLSEMVKDNLLDTSNYPSTHPLYSKKL 1019

HSP 5 Score: 70.4774 bits (171), Expect = 5.617e-15
Identity = 32/64 (50.00%), Postives = 46/64 (71.88%), Query Frame = 1 
Query:  205 KVHLGRGWRVINILEIDFILTKFSPISRLSGHRFIQTPSAIISKKAIINVKNTNDKCVLYSILA 396
            +  LG  W++  I+E++F +TK S ISRLSGH +IQTP A++ KKAI+NV+NT+      S+LA
Sbjct:  288 RTELGSSWKINRIIEVNFKVTKVSNISRLSGHSYIQTPKALMGKKAIVNVQNTDKFMFHISVLA 351

HSP 6 Score: 30.8018 bits (68), Expect = 5.617e-15
Identity = 22/55 (40.00%), Postives = 29/55 (52.73%), Query Frame = 2 
Query:   50 IKIMLTERISSKLI----NEIGKMGEILDNSFSNYTEKFSDSFLSEGVNKLNEKI 202
            +KI L E   SK+      E  K GE+   S SN     + +FL EG+ K+NEKI
Sbjct:  231 LKITLNEMKHSKVQFGVQVEFEKDGELKVVSISNRAASVTTTFLDEGMKKINEKI 285

HSP 7 Score: 53.9138 bits (128), Expect = 4.394e-12
Identity = 28/64 (43.75%), Postives = 37/64 (57.81%), Query Frame = 2 
Query: 1070 MTFAEELQFQSCSTCHICKKPI-NTC*ILR*FLW*ISGPACRKFNLSRRIQRQLSVVFHNLRGY 1258
            +TF EE  F +C  C++C K I +       F     GPAC+  N++R I+  L VVFHNLRGY
Sbjct:  534 LTFDEEYSFDNCEYCYLCNKNIVDKVRDHDHFTGKYIGPACKSCNVNRSIKELLPVVFHNLRGY 597

HSP 8 Score: 37.7354 bits (86), Expect = 4.394e-12
Identity = 18/36 (50.00%), Postives = 22/36 (61.11%), Query Frame = 1 
Query:  952 YFEFYEPDCIPLFLKKVEEIAVTIVLPYYEIEGKIL 1059
            Y  F   +C+  FLKKV +I   IVLPYY+  GK L
Sbjct:  495 YHHFIGQNCVYEFLKKVSDIGTNIVLPYYKENGKKL 530

HSP 9 Score: 62.7734 bits (151), Expect = 1.800e-9
Identity = 71/206 (34.47%), Postives = 94/206 (45.63%), Query Frame = 3 
Query: 2229 SYIRQSVLDLSKQRMYQLQHIELEKYRKLFNCQ-KXXXXXXXXXFLEN*NSKLDFHLPALIC----------WTXXXXXXXXXXXXGTCDRKV*R*ELRSWIH*MGFLVSKMLSSVRKQIIDESQGCNI--TW*TYKTYLDVY-NNSTV------VCTSD*NRNXXXXXXXXXXXXNHDDKRAWVGKYTSFPYGDYISAYVIDIDN 2786
            SYI QSVLDLSK RMY+LQ+ +LEKYR+ FNC+  I        FLE  N KLD  L  ++            T  +    +  +IG    +        W+    FL  K  S + K    +++G  +  T  T+K+Y D Y  NST       + TS  N    S       L N +DKR WV K  S  YG Y+    I+ DN
Sbjct:  938 SYIGQSVLDLSKLRMYELQYKDLEKYRRQFNCEINIVAGDTDSFFLEIKNCKLDILLSEMVKDNLLDTSNYPSTHPLYSKKLDSVIGKFKDESKGEGYDEWV----FLRPKCYSLLGKSKCLKAKGVTLKDTTLTHKSYKDCYEKNSTFSVPQFRIGTS--NHQLYSFKTSKVALTNKEDKRVWVEKNVSLAYGHYM----IETDN 1133
The following BLAST results are available for this feature:
BLAST of Rho_N domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho_N domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ZC53.43.128e-1936.89pep chromosome:WBcel235:X:1925750:1937689:1 gene:W... [more]
H12I13.36.073e-1036.36pep chromosome:WBcel235:II:6071386:6073251:-1 gene... [more]
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BLAST of Rho_N domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho_N domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho_N domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho_N domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho_N domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho_N domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1Y1MY913.376e-3138.66Uncharacterized protein (Fragment) OS=Photinus pyr... [more]
A0A2L2YKB91.560e-3033.13Uncharacterized protein (Fragment) OS=Parasteatoda... [more]
A0A023EZN11.874e-3033.33Putative dna polymerase type b (Fragment) OS=Triat... [more]
A0A224XH841.180e-2944.07Putative dna polymerase type b (Fragment) OS=Panst... [more]
A0A4Y2PKA12.807e-2938.79Uncharacterized protein OS=Araneus ventricosus OX=... [more]
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BLAST of Rho_N domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho_N domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho_N domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho_N domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO461094.435e-2540.68Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO295948.504e-1328.32Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO311411.165e-1228.57Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO441102.481e-932.48Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO480213.083e-935.04Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Rho_N domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rho_N domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000040848.11.738e-2565.66SMESG000040848.1[more]
SMESG000040848.11.675e-15566.33SMESG000040848.1[more]
SMESG000061056.12.114e-13981.12SMESG000061056.1[more]
SMESG000076702.16.499e-13738.10SMESG000076702.1[more]
SMESG000076702.15.544e-13441.87SMESG000076702.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024373 ID=SMED30024373|Name=Rho_N domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=3696bp
AAACGGCGCAATTATAGCAATTAAATTTGATACATCAGAGGCGCGACTGA
TAAAAATAATGTTAACTGAGAGAATCAGTTCTAAATTAATTAATGAGATT
GGAAAGATGGGGGAAATCCTCGACAATTCTTTTTCCAACTACACAGAAAA
ATTTTCAGATAGTTTTTTATCTGAAGGAGTTAATAAACTCAATGAAAAAA
TAAAAAAGTTCATTTAGGCAGGGGATGGCGAGTAATCAATATTTTAGAAA
TAGATTTTATACTTACCAAATTTTCCCCAATTAGTCGTTTATCAGGTCAT
CGATTTATTCAGACTCCTTCTGCTATTATATCTAAAAAAGCTATTATAAA
TGTTAAAAATACCAACGATAAATGCGTCCTTTATAGTATTCTGGCTTAGA
ATATGACGAAATTACTAAATATCTCCAAATTTGAAAAGAAAATGCCAGTT
TAAATTTAGCAATAAACGTCCTTTTTTATACTCCTATTGTGATAATTGAC
GAGGTTCCTGATAGTTATGTCTTCCATCACCCTAATCGTTCAAAGGTCAC
TGACGAAACACCTATTTATTTGTGTTTATTAGAGAAAAGTGATAGGAACC
ACTACACCGCGGTAAGTGATCTCAATAAATTAACGAATTGTCATCAGAAC
CCAGATTGTCCAGTTCAAATTAGATGCGAATGGTGCCCACGCTGCCTAAA
TAGTTTCAGGCTGGATAATGCCTTTAATAAACATGTATCTCTATGCGCTA
AAAATCAGATGGGTACAACCCTCTACACATTATCCGATAACAAAGATTTA
AAATTTGAAAATTATTCTAAAACCATCACCCCTCCCTATGTAATTTATGC
CGATTTCGAGTCGGTTTTACCACATGATGTTAGACATCACCAAATTCATA
TGCCTAGTTCAGCAGGCCTTTTACTATTAAATAACTACACAAAAACAGCA
ATATTTTGAATTTTATGAACCTGACTGCATTCCTTTATTTCTTAAAAAAG
TAGAGGAAATTGCTGTCACAATTGTCCTTCCATATTATGAAATTGAAGGT
AAAATTTTGTTTGAAATCCATGACTTTTGCAGAGGAACTGCAATTTCAAT
CTTGTAGTACTTGTCACATATGTAAAAAACCTATTAATACATGTTAGATA
TTACGATAATTTCTCTGGTAAATATCTGGGCCAGCCTGTAGAAAATTTAA
TTTGTCTCGCAGAATTCAAAGACAATTATCTGTCGTATTTCATAATTTGA
GAGGATATGTATCATATTTTAAAATATGGAATTGATGAATTTCCTTATTG
GGAATTGAATATTATTCCAACTAGCACCAAATTGCCTCCTATTTGGTCTA
ATGTTAGTGAAGATGAGTACGCTAAGGCACAGTTAATTTGGAATCAACAA
AACTGTAATACTTTGTTAGATAACATGCTAGCATATCTTAAATTAGATGT
ATTCATCCTTGCGAAATATTTTCAACAATTTAGTACAAAAGCCATTGCAT
ATAATGGTATTGAACATTTAGGTTTTTTACGGAATTCCTGGTATGTCGTG
GGTAATGTCATTAACGGAACCTATTGAACTTTTACAGGATATGAAAATGT
TTGATTTCTTTGAGGGAGAAATGCTAGGAGAGTTAACATATGTCAACAAA
CATTACGCTGCGACTTCAGAATATATAGAGTTATTATATATTGATATAAA
TAATGTTTATGGTTGGGCTCTTAGCCAAAATCTTCCGTTTAGTGATTTTA
AATGGGTTCATTATCGTATGAATGATGTTTTACAAGAGTCTATGAATGAG
GAAATCGAACTCCTTCCACATGGTAACACTATGGAGGTCGACATCGATAT
TCCAGATAGCTTACATAACTTTCCACTTGCTCCGGAAAAATTATGTTCTT
CATTAGAGTCTTCTGAAACTTTACTTGTCTCTTGGTGAAAAAGTTGTAAA
TATTCATATAACAATCAGATTCAAGCAATCACCAATATTTAAAAAATATC
TTGACACAAATATTAATTTGCGTGCTAGTGCAGTAAGTGAGCTTTGTAGA
GATTTGTATAAATTATACAACAATAGCCTATACGGCAAAACAGTAGAAAA
CCTAAAAATGCGAATGAATTTGTGGTTGTTAAATAATTCAACTGAAACGT
TGATTACATATTCATCAAAACTTTCATTTAGAAAAACCATTAAAATCGAT
GAAAACTTGTTTGCTGCACATCTCAATAAGCTACATTAGACAGTCTGTAC
TGGATTTATCCAAACAACGAATGTATCAACTCCAACACATTGAATTGGAG
AAATATAGGAAACTATTTAATTGCCAAAAAATATAGTAACTGGTGATATT
GATAGTTTTTTTGGAGAATTAGAATTCAAAGCTCGATTTTCATTTGCCAG
CGTTGATTTGTTGGACACTCCGAATTATGATCCTTCTCATCCTTTATATT
ATAGGAACCTGTGATCGGAAAGTTTAAAGATGAGAGCTAAGGAGTTGGAT
ACACTGAATGGGTTTTCTTGTGTCCAAAATGTTATCATCTGTTAGGAAAC
AAATCATCGATGAAAGCCAAGGGTGTAACATTACCTGGTAAACATATAAA
ACATATTTAGATGTTTACAACAATAGCACAGTTGTCTGTACCTCAGACTA
GAATCGGAACTAGAAATCATCATCTTTATACATTTAAAAACTCAAAAATC
ATGATGATAAGAGAGCTTGGGTAGGAAAATACACAAGTTTTCCATATGGG
GATTATATTAGTGCTTATGTGATTGATATTGACAATAATATTACTAATCA
TTACTAATCATTATAGATATATAGGTATACCAATTTTTTATCAAATAATG
CAGTAAAATCAATTTAATAAGTCACAGAATGGGTCATAACATTGTCACAA
TCATTATCGTTACCATCACAATTATCATAATCCATCGTTTGATATATATG
TTCATTCTATATTTCTCGTACCCTACTATGTACCTCTATGACTTGCCCAT
CTTCCAACATAATCAAACATTGCAATCAGTCTTGTTTTTCATCAAATTTA
CACTAAACTGCGTTGAGATACATTTTCTATAACAGCAATGTAGAACTTAT
GAGGCACGCATGTATTTTTATTTTTTGAAAATTTATACCACCAACCATGT
TTCTTTTTGAACAACCCTATTCAGAAGAACATCATGCTTGTAAGAAACAA
ATTTAATTCTTCTGATACTTGTCAGTCTTTAAAGAAGGATTTTTTTATTC
ATAAAATGCCACAATTTTTTCGTTGTTTTAATATTCAGTCTCCCATGGTG
GAAAAATTATCTCAAAACTTAATAAATTTCTATTTTAAATAAAATTATGT
TAATGCAAATATATTTAAAAATGTAAATGTGAAAGTAATGTTATAAAATT
GGGGAATAGAAAACTAATTTTCTTGACAAAAGGCAGATTCAGAGAATATT
AAAATGTCGCCTTAAATATTATTGAATTGACAATATCATATCATATTATA
TCTAATCAAGATTGATAACTCCCCAGCGTTAACTAATCCAAATGAAGACA
AATGGAATAATATCGACAAGCCTCCAGAAAACGACTCGCCAATCTCTCGA
CCGAAATCTATTGAACTTAATTCTATTAAATTGCTATATTCTAAAATTAC
ACAAACTGATTTTGTTTGAATAAATTGGTTGATTTATTTCAAAAAA
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protein sequence of SMED30024373-orf-1

>SMED30024373-orf-1 ID=SMED30024373-orf-1|Name=SMED30024373-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=132bp
MSWVMSLTEPIELLQDMKMFDFFEGEMLGELTYVNKHYAATSEYIELLYI
DINNVYGWALSQNLPFSDFKWVHYRMNDVLQESMNEEIELLPHGNTMEVD
IDIPDSLHNFPLAPEKLCSSLESSETLLVSW*
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protein sequence of SMED30024373-orf-2

>SMED30024373-orf-2 ID=SMED30024373-orf-2|Name=SMED30024373-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=107bp
MYHILKYGIDEFPYWELNIIPTSTKLPPIWSNVSEDEYAKAQLIWNQQNC
NTLLDNMLAYLKLDVFILAKYFQQFSTKAIAYNGIEHLGFLRNSWYVVGN
VINGTY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0006353DNA-templated transcription, termination
GO:0006260DNA replication
GO:0071897DNA biosynthetic process
GO:0090305nucleic acid phosphodiester bond hydrolysis
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR004868DNA-dir_DNA_pol_B_mt/vir
Vocabulary: molecular function
TermDefinition
GO:00084083'-5' exonuclease activity
GO:0003677DNA binding
GO:0000166nucleotide binding
GO:0003887DNA-directed DNA polymerase activity
GO:0003676nucleic acid binding