SMED30024348

Overview
NameSMED30024348
Smed IDSMED30024348
Length (bp)962
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30024348 (SMED30024348) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30024348 (SMED30024348) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30024348

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30024348h1SMcG0012649 SmedASXL_000510SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30024348h1SMcG0012649 SmedASXL_019401SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30024348h1SMcG0012649 dd_Smed_v4_19769_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30024348h1SMcG0012649 dd_Smed_v6_15797_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30024348h1SMcG0012649 dd_Smed_v6_19769_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30024348 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002389:22292..28426 +4719
v31.000100:88409..96805 -3648
v31.004174:16695..18701 +2675
Homology
[Close]
BLAST of SMED30024348 vs. Planmine SMEST
Match: SMESG000074227.1 (SMESG000074227.1)

HSP 1 Score: 540.421 bits (1391), Expect = 0.000e+0
Identity = 261/261 (100.00%), Postives = 261/261 (100.00%), Query Frame = 3 
Query:    3 IFLNTQRIKKDGMVSVKLLNGQANLKGTLKWHGSMNNVPVCAVELLQEHENGTNGRFWRTRLFECRDKLAIATLQQNLSPIKSELKFIKEINLNVVDPFNVREIENGRQINKSTCYKINGAVKILNIKWRGIVDGELCVGCELSGPVECGTDGKFGNKNLFKFPDRRGVLIPTGELRDSCESTAHSEIAILPGRGWPRKETTYFIDPDCICCIELTELLQQHPDLRIKSRIKIHNIENKQIGGLCEWIGLINGQEALGITM 785
            IFLNTQRIKKDGMVSVKLLNGQANLKGTLKWHGSMNNVPVCAVELLQEHENGTNGRFWRTRLFECRDKLAIATLQQNLSPIKSELKFIKEINLNVVDPFNVREIENGRQINKSTCYKINGAVKILNIKWRGIVDGELCVGCELSGPVECGTDGKFGNKNLFKFPDRRGVLIPTGELRDSCESTAHSEIAILPGRGWPRKETTYFIDPDCICCIELTELLQQHPDLRIKSRIKIHNIENKQIGGLCEWIGLINGQEALGITM
Sbjct:   34 IFLNTQRIKKDGMVSVKLLNGQANLKGTLKWHGSMNNVPVCAVELLQEHENGTNGRFWRTRLFECRDKLAIATLQQNLSPIKSELKFIKEINLNVVDPFNVREIENGRQINKSTCYKINGAVKILNIKWRGIVDGELCVGCELSGPVECGTDGKFGNKNLFKFPDRRGVLIPTGELRDSCESTAHSEIAILPGRGWPRKETTYFIDPDCICCIELTELLQQHPDLRIKSRIKIHNIENKQIGGLCEWIGLINGQEALGITM 294

HSP 2 Score: 78.5666 bits (192), Expect = 7.331e-16
Identity = 37/37 (100.00%), Postives = 37/37 (100.00%), Query Frame = 1 
Query:  829 DKDVKGTNGIFSCFRMFTCDKGKGVLVLADNIIHGIL 939
            DKDVKGTNGIFSCFRMFTCDKGKGVLVLADNIIHGIL
Sbjct:  295 DKDVKGTNGIFSCFRMFTCDKGKGVLVLADNIIHGIL 331

HSP 3 Score: 51.9878 bits (123), Expect = 3.890e-7
Identity = 24/46 (52.17%), Postives = 31/46 (67.39%), Query Frame = 3 
Query:   99 GSMNNVPVCAVELLQEHENGTNGRFWRTRLFECRDKLAIATLQQNL 236
            G MN + VC +EL Q+HENG+NG FWR R+ + R K  IAT   N+
Sbjct:  483 GWMNKLLVCVIELDQDHENGSNGIFWRRRIVDSRGKRVIATHLNNV 528
[Close]
BLAST of SMED30024348 vs. Planmine SMEST
Match: SMESG000074227.1 (SMESG000074227.1)

HSP 1 Score: 540.421 bits (1391), Expect = 0.000e+0
Identity = 261/261 (100.00%), Postives = 261/261 (100.00%), Query Frame = 3 
Query:    3 IFLNTQRIKKDGMVSVKLLNGQANLKGTLKWHGSMNNVPVCAVELLQEHENGTNGRFWRTRLFECRDKLAIATLQQNLSPIKSELKFIKEINLNVVDPFNVREIENGRQINKSTCYKINGAVKILNIKWRGIVDGELCVGCELSGPVECGTDGKFGNKNLFKFPDRRGVLIPTGELRDSCESTAHSEIAILPGRGWPRKETTYFIDPDCICCIELTELLQQHPDLRIKSRIKIHNIENKQIGGLCEWIGLINGQEALGITM 785
            IFLNTQRIKKDGMVSVKLLNGQANLKGTLKWHGSMNNVPVCAVELLQEHENGTNGRFWRTRLFECRDKLAIATLQQNLSPIKSELKFIKEINLNVVDPFNVREIENGRQINKSTCYKINGAVKILNIKWRGIVDGELCVGCELSGPVECGTDGKFGNKNLFKFPDRRGVLIPTGELRDSCESTAHSEIAILPGRGWPRKETTYFIDPDCICCIELTELLQQHPDLRIKSRIKIHNIENKQIGGLCEWIGLINGQEALGITM
Sbjct:   34 IFLNTQRIKKDGMVSVKLLNGQANLKGTLKWHGSMNNVPVCAVELLQEHENGTNGRFWRTRLFECRDKLAIATLQQNLSPIKSELKFIKEINLNVVDPFNVREIENGRQINKSTCYKINGAVKILNIKWRGIVDGELCVGCELSGPVECGTDGKFGNKNLFKFPDRRGVLIPTGELRDSCESTAHSEIAILPGRGWPRKETTYFIDPDCICCIELTELLQQHPDLRIKSRIKIHNIENKQIGGLCEWIGLINGQEALGITM 294

HSP 2 Score: 75.0998 bits (183), Expect = 1.264e-14
Identity = 34/34 (100.00%), Postives = 34/34 (100.00%), Query Frame = 1 
Query:  829 DKDVKGTNGIFSCFRMFTCDKGKGVLVLADNIIH 930
            DKDVKGTNGIFSCFRMFTCDKGKGVLVLADNIIH
Sbjct:  295 DKDVKGTNGIFSCFRMFTCDKGKGVLVLADNIIH 328

HSP 3 Score: 51.9878 bits (123), Expect = 3.861e-7
Identity = 24/46 (52.17%), Postives = 31/46 (67.39%), Query Frame = 3 
Query:   99 GSMNNVPVCAVELLQEHENGTNGRFWRTRLFECRDKLAIATLQQNL 236
            G MN + VC +EL Q+HENG+NG FWR R+ + R K  IAT   N+
Sbjct:  460 GWMNKLLVCVIELDQDHENGSNGIFWRRRIVDSRGKRVIATHLNNV 505
[Close]
BLAST of SMED30024348 vs. Planmine SMEST
Match: SMESG000007087.1 (SMESG000007087.1)

HSP 1 Score: 325.479 bits (833), Expect = 1.195e-101
Identity = 167/245 (68.16%), Postives = 185/245 (75.51%), Query Frame = 3 
Query:   51 KLLNGQANLKGTLKWHGSMNNVPVCAVELLQEHENGTNGRFWRTRLFECRDKLAIATLQQNLSPIKSELKFIKEINLNVVDPFNVREIENGRQINKSTCYKINGAVKILNIKWRGIVDGELCVGCELSGPVECGTDGKFGNKNLFKFPDRRGVLIPTGELRDSCESTAHSEIAILPGRGWPRKETTYFIDPDCICCIELTELLQQHPDLRIKSRIKIHNIENKQIGGLCEWIGLINGQEALGITM 785
            KL N ++N K   KW G +N+V V  VE+ +  E GT+G F +  LF    K       +     KSELK +KEINLNVVDPFNVREIENGRQINK TCYK  GA+KILNIKWRGIV+GELCVGCELSGPVE GTDG FGNK +FK P RRGVLIPT EL DSCE+TAHS+I  LP  GW  K  TYFI+PDCICC E TE LQ+HPDLRIKSRIK+ N ENKQIGG CEWIGLINGQEALGITM
Sbjct:  878 KLKNIKSNKKAIWKWSGLVNDVEVIGVEMNESLEKGTDGNFCKIPLFRSVTKRGALFKSEEFINSKSELKHVKEINLNVVDPFNVREIENGRQINKLTCYKSKGAIKILNIKWRGIVNGELCVGCELSGPVEWGTDGTFGNKIVFKCPARRGVLIPTEELIDSCETTAHSKITTLPNSGWLWKGDTYFINPDCICCTESTESLQKHPDLRIKSRIKVPNKENKQIGGSCEWIGLINGQEALGITM 1122

HSP 2 Score: 63.929 bits (154), Expect = 1.195e-101
Identity = 28/34 (82.35%), Postives = 31/34 (91.18%), Query Frame = 1 
Query:  829 DKDVKGTNGIFSCFRMFTCDKGKGVLVLADNIIH 930
            D DVKGTNGIFSCFRMFTC+ GKGVL+LA+NI H
Sbjct: 1123 DNDVKGTNGIFSCFRMFTCESGKGVLMLAENIFH 1156

HSP 3 Score: 151.754 bits (382), Expect = 1.458e-40
Identity = 82/132 (62.12%), Postives = 94/132 (71.21%), Query Frame = 3 
Query:    9 LNTQRIKKDGMVSVKLLNGQANLKGTLKWHGSMNNVPVCAVELLQEHENGTNGRFWRTRLFECRDKLAIATLQQNLSPIKSELKFIKEINLNVVDPFNVREIENGRQINKSTCYKINGAVKILNIKWRGIVD 404
            +  QRIKKDGMV+VKLLNGQANLKGTLKWHG MNNVPVCAV+LLQEHENGTNGRFWRTRLFECRDKLAIATL +NLSP    +  IK+I +      N +E   G  +     Y +   VKIL     G ++
Sbjct:  373 IKAQRIKKDGMVNVKLLNGQANLKGTLKWHGLMNNVPVCAVQLLQEHENGTNGRFWRTRLFECRDKLAIATLTKNLSP-SMPIDLIKDIFVEK----NQKEFSRG-SVRTDDIYHVGQFVKILPFDLFGQIE 498

HSP 4 Score: 94.3597 bits (233), Expect = 4.471e-21
Identity = 68/204 (33.33%), Postives = 95/204 (46.57%), Query Frame = 3 
Query:    6 FLNTQRIKKDGMVSVKLLNGQANLKGTLKWHGSMNNVPVCAVELLQEHENGTNGRFWRTRLFECRDKLAIATLQQNLSPIKSELKFIKEINLNVVDPFNVREIENGRQINKSTCYKINGAVKIL------------NIKWRGIVDGE-LCVGCELSGPVECGTDGKFGNKNLFKFPDRRGVLIP----TGELRDSCESTAHSEI 566
            +L+ + IK +  V VK  N +  LKG L+W G MN + VC +EL Q+HENG+NG FWR R+   R K  IAT       +    K+         +P+  + I    QI K     IN AVK               +KW G+ + +    G EL   ++ GTDG FG + LF   +  GVL P      + R + + T H E 
Sbjct: 1295 YLDDKNIKMNEFVFVKQENPELKLKGKLRWIGWMNKLLVCVIELDQDHENGSNGTFWRRRIVNSRGKRVIAT------SVNHVYKY---------EPYLSKAIPEEIQIFKEIV--INSAVKYYFLTATGYQDLFGTVKWTGVDENDCYFFGLELDDNIDIGTDGTFGGQRLFTCQNGHGVLAPLNTVASDTRFTAKETQHEEF 1481

HSP 5 Score: 77.0258 bits (188), Expect = 3.406e-15
Identity = 67/256 (26.17%), Postives = 114/256 (44.53%), Query Frame = 3 
Query:   42 VSVKLLNGQANLKGTLKWHGSMN-NVPVCAVELLQEHENGTNGRFWRTRLFECRD-KLAIATLQQNLSPIKSELKFIKEI-----NLNVVDPFNVREIEN-GRQINKSTCYKINGAVKILNIKWRGIVDGELCVGCELSGPVECGTDGKFGNKNLFKFPDRRGVLIPTGELRDSCESTAHSEIAILPGRGWPRKETTYFIDPDCICCIELTELLQQHPDLRIKSRIKIHNIENKQIGGLCEWIGLINGQEALGITM 785
            V    ++ +++ KG +K  G  N  +P C V++    E   N    +  L E  + K+   T    L  I++ L    E+      + V+DP  +R IE    +  K   +  N     + +KW+G++D + C+GC  +     GT+G FG   LF   D+ G+LIP   + + CE   H  I       W R      ID + ICC     +       + ++  K+ NI++ +   + +W GL+N  E +G+ M
Sbjct:  668 VEFMSMDRKSSYKGRIKSTGIANKTIPSCVVQM-PPSEPIVNRSLLQYTLNENLEFKILSGTANDFLFLIRANLLRKSELPETVNGIPVIDPEFIRIIEGVAVEPGKFIVFTCNNQRHSVQLKWKGLIDQKYCLGCVANFQCNFGTNGTFGQDKLFACEDKHGILIPLAAVSECCEMKGHKPIK------WTRDGA---IDENSICCTVEKNI------TKFQANCKLKNIKSNK-KAIWKWSGLVNDVEVIGVEM 906

HSP 6 Score: 69.707 bits (169), Expect = 8.405e-13
Identity = 54/173 (31.21%), Postives = 85/173 (49.13%), Query Frame = 3 
Query:  270 EINLNVVDPFNVREIEN--GRQINKSTCYKINGAVK-ILNIKWRGIVDGELCVGCELSGPVECGTDGKFGNKNLFKFPDRRGVLIPTGELRDSCESTAHSEIAILPGRGWPRKETTYFIDPDCICCIELTELLQQHPDLRIKSRIKIHNIENKQIGGLCEWIGLINGQEALGI 779
            ++ L+V+ P  + +I    G Q+NK     I G  K  + ++W+G ++   C+GCEL      GT+G FG   LF     RGVLI   +   SCES   S    L             +  + ICC E  + L +  D+++ S++    +   + GG C+W+GLING+  +GI
Sbjct:   48 KLGLDVIKPGAIDQIGKLKGAQMNKYIA--IQGKTKSFVKLQWKGKINNINCLGCELPEFWSEGTEGTFGRYKLFTCSYGRGVLILINDAVKSCESAEESNHLPLADNDE--------LTANNICCFEPLDKLSK--DIKVNSQVFCQQL---KAGGKCKWMGLINGKGYVGI 205

HSP 7 Score: 54.299 bits (129), Expect = 7.452e-8
Identity = 57/241 (23.65%), Postives = 108/241 (44.81%), Query Frame = 3 
Query:   81 GTLKWHGSMNNVPVCAVELLQEHENGTNGRFWRTRLFECRDKLAIATLQQNLSPIKSELK--FIKE--INLNVVDPFNVREIENGRQINKSTCYKINGAVK-----ILNIKWRGIVDGELCVGCEL-SGPVECGTDGKFGNKNLFKFPDRRGV--LIPTGELRDSCESTAHSEIAILPGRGWPR---KETTYFIDPDCICCIELTELLQQHPDLRIKSRIKIHNIENKQIGGLCEWIGLIN 758
            G+ +W G +N      + +  + + GTNG F   R+F C     +  L +N+   K   K  FI +  +++N +   +  E ENG  I K     +N         I  +KW G ++ E CVG ++    +E G DG  G   +F+  ++ G+  L+   EL ++ E+    +  ++    + R   + +  ++D   I   E   + Q++P+L++K +++              WIG +N
Sbjct: 1104 GSCEWIGLINGQEALGITMDNDVK-GTNGIFSCFRMFTCESGKGVLMLAENIFHDKEREKPQFIADKIVDINKIYSISPCEGENGIGIGKPIKINLNSPNGRNRDFIGEVKWTGRINDEKCVGLDMVEEEMEHGMDGMIGKSRIFQCMEKHGLVALLSDVELSENAENPQILDSGMIAPNKFIRFQEERSKKYLDDKNIKMNEFVFVKQENPELKLKGKLR--------------WIGWMN 1329
[Close]
BLAST of SMED30024348 vs. Planmine SMEST
Match: SMESG000044783.1 (SMESG000044783.1)

HSP 1 Score: 295.434 bits (755), Expect = 1.847e-94
Identity = 145/180 (80.56%), Postives = 156/180 (86.67%), Query Frame = 3 
Query:  246 KSELKFIKEINLNVVDPFNVREIENGRQINKSTCYKINGAVKILNIKWRGIVDGELCVGCELSGPVECGTDGKFGNKNLFKFPDRRGVLIPTGELRDSCESTAHSEIAILPGRGWPRKETTYFIDPDCICCIELTELLQQHPDLRIKSRIKIHNIENKQIGGLCEWIGLINGQEALGITM 785
            KSELK++KEINLNVVDPFNVREIENGR+INK T YK  GA+KILNIKWRGIV+GELCVGCELSGPVE GTDG FGNK +FK P RRGVLIPT EL DSCE+TAHS+I  LP  GW  K  TYFI+PDCICC E TELLQ+HPDLRIKSRIK+ N ENKQIGG CEWIGLINGQEALGITM
Sbjct:  317 KSELKYVKEINLNVVDPFNVREIENGRRINKLTRYKSKGAIKILNIKWRGIVNGELCVGCELSGPVEWGTDGTFGNKIVFKCPARRGVLIPTEELIDSCETTAHSKITTLPDSGWWWKGDTYFINPDCICCTESTELLQKHPDLRIKSRIKVPNKENKQIGGSCEWIGLINGQEALGITM 496

HSP 2 Score: 61.2326 bits (147), Expect = 3.905e-10
Identity = 27/34 (79.41%), Postives = 30/34 (88.24%), Query Frame = 1 
Query:  829 DKDVKGTNGIFSCFRMFTCDKGKGVLVLADNIIH 930
            D DVKGTNGIFSC RMFTC+ GKGVL+LA+NI H
Sbjct:  497 DNDVKGTNGIFSCLRMFTCEFGKGVLMLAENIFH 530

HSP 3 Score: 51.9878 bits (123), Expect = 3.166e-7
Identity = 55/240 (22.92%), Postives = 106/240 (44.17%), Query Frame = 3 
Query:   81 GTLKWHGSMNNVPVCAVELLQEHENGTNGRFWRTRLFECRDKLAIATLQQNLSPIKSELK--FIKE--INLNVVDPFNVREIENGRQINKSTCYKINGAVK-----ILNIKWRGIVDGELCVGCELSGPVECGTDGKFGNKNLFKFPDRRGV--LIPTGELRDSCESTAHSEIAILPGRGWPR---KETTYFIDPDCICCIELTELLQQHPDLRIKSRIKIHNIENKQIGGLCEWIGLIN 758
            G+ +W G +N      + +  + + GTNG F   R+F C     +  L +N+   K   K  FI +  +++N +      E ENG  I K     +N         I  +KW G ++ E CVG ++   +E G DG  G   +F+  ++ G+  L+   EL ++ E+    +  ++    + R   + +  +++   I   E   + Q++ +L++K +++              WIG +N
Sbjct:  478 GSCEWIGLINGQEALGITMDNDVK-GTNGIFSCLRMFTCEFGKGVLMLAENIFHDKESEKPQFIADKIVDINKIYSIFPCEGENGISIGKPIKINLNSPNGRKRDFIGEVKWTGRINDEKCVGLDMEEEMEHGMDGMIGKSRIFQCMEKHGLVALLSDVELSENAENPQVLDSGMIAPNKFIRFQEERSKKYLEDKNIKMNEFVFVKQENSELKLKGKLR--------------WIGWMN 702
[Close]
BLAST of SMED30024348 vs. Planmine SMEST
Match: SMESG000044783.1 (SMESG000044783.1)

HSP 1 Score: 296.59 bits (758), Expect = 8.509e-93
Identity = 145/180 (80.56%), Postives = 156/180 (86.67%), Query Frame = 3 
Query:  246 KSELKFIKEINLNVVDPFNVREIENGRQINKSTCYKINGAVKILNIKWRGIVDGELCVGCELSGPVECGTDGKFGNKNLFKFPDRRGVLIPTGELRDSCESTAHSEIAILPGRGWPRKETTYFIDPDCICCIELTELLQQHPDLRIKSRIKIHNIENKQIGGLCEWIGLINGQEALGITM 785
            KSELK++KEINLNVVDPFNVREIENGR+INK T YK  GA+KILNIKWRGIV+GELCVGCELSGPVE GTDG FGNK +FK P RRGVLIPT EL DSCE+TAHS+I  LP  GW  K  TYFI+PDCICC E TELLQ+HPDLRIKSRIK+ N ENKQIGG CEWIGLINGQEALGITM
Sbjct:  317 KSELKYVKEINLNVVDPFNVREIENGRRINKLTRYKSKGAIKILNIKWRGIVNGELCVGCELSGPVEWGTDGTFGNKIVFKCPARRGVLIPTEELIDSCETTAHSKITTLPDSGWWWKGDTYFINPDCICCTESTELLQKHPDLRIKSRIKVPNKENKQIGGSCEWIGLINGQEALGITM 496

HSP 2 Score: 63.1586 bits (152), Expect = 8.509e-93
Identity = 27/34 (79.41%), Postives = 30/34 (88.24%), Query Frame = 1 
Query:  829 DKDVKGTNGIFSCFRMFTCDKGKGVLVLADNIIH 930
            D DVKGTNGIFSC RMFTC+ GKGVL+LA+NI H
Sbjct:  497 DNDVKGTNGIFSCLRMFTCEFGKGVLMLAENIFH 530

HSP 3 Score: 72.4034 bits (176), Expect = 9.422e-14
Identity = 41/101 (40.59%), Postives = 56/101 (55.45%), Query Frame = 3 
Query:    6 FLNTQRIKKDGMVSVKLLNGQANLKGTLKWHGSMNNVPVCAVELLQEHENGTNGRFWRTRLFECRDKLAIATLQQNL--------SPIKSELKFIKEINLN 284
            +L  + IK +  V VK  N +  LKG L+W G MN + VC +EL Q+HENG+NG FWR R+   R K  IAT   ++          I  E++  KEI +N
Sbjct:  668 YLEDKNIKMNEFVFVKQENSELKLKGKLRWIGWMNKLLVCVIELDQDHENGSNGTFWRRRIVNSRGKRVIATNINHVYKYETYVSKAIPEEIQIFKEIVIN 768

HSP 4 Score: 51.6026 bits (122), Expect = 4.793e-7
Identity = 55/240 (22.92%), Postives = 106/240 (44.17%), Query Frame = 3 
Query:   81 GTLKWHGSMNNVPVCAVELLQEHENGTNGRFWRTRLFECRDKLAIATLQQNLSPIKSELK--FIKE--INLNVVDPFNVREIENGRQINKSTCYKINGAVK-----ILNIKWRGIVDGELCVGCELSGPVECGTDGKFGNKNLFKFPDRRGV--LIPTGELRDSCESTAHSEIAILPGRGWPR---KETTYFIDPDCICCIELTELLQQHPDLRIKSRIKIHNIENKQIGGLCEWIGLIN 758
            G+ +W G +N      + +  + + GTNG F   R+F C     +  L +N+   K   K  FI +  +++N +      E ENG  I K     +N         I  +KW G ++ E CVG ++   +E G DG  G   +F+  ++ G+  L+   EL ++ E+    +  ++    + R   + +  +++   I   E   + Q++ +L++K +++              WIG +N
Sbjct:  478 GSCEWIGLINGQEALGITMDNDVK-GTNGIFSCLRMFTCEFGKGVLMLAENIFHDKESEKPQFIADKIVDINKIYSIFPCEGENGISIGKPIKINLNSPNGRKRDFIGEVKWTGRINDEKCVGLDMEEEMEHGMDGMIGKSRIFQCMEKHGLVALLSDVELSENAENPQVLDSGMIAPNKFIRFQEERSKKYLEDKNIKMNEFVFVKQENSELKLKGKLR--------------WIGWMN 702
The following BLAST results are available for this feature:
BLAST of SMED30024348 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024348 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024348 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024348 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024348 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024348 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024348 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024348 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024348 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024348 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024348 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024348 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024348 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024348 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000074227.17.331e-16100.00SMESG000074227.1[more]
SMESG000074227.11.264e-14100.00SMESG000074227.1[more]
SMESG000007087.11.195e-10168.16SMESG000007087.1[more]
SMESG000044783.11.847e-9480.56SMESG000044783.1[more]
SMESG000044783.18.509e-9380.56SMESG000044783.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024348 ID=SMED30024348|Name=SMED30024348|organism=Schmidtea mediterranea sexual|type=transcript|length=962bp
ATATATTTCTAAACACGCAACGAATAAAGAAAGATGGAATGGTGAGTGTG
AAGCTATTAAATGGGCAAGCAAATCTGAAAGGGACATTGAAATGGCATGG
CTCAATGAATAATGTTCCCGTCTGTGCTGTGGAATTGTTACAAGAGCATG
AAAATGGAACAAATGGCCGATTTTGGAGGACGCGGTTGTTTGAATGCAGG
GATAAACTGGCAATTGCTACTCTGCAACAGAATTTATCTCCAATAAAATC
TGAATTGAAATTCATCAAAGAAATTAATTTAAATGTTGTTGATCCATTTA
ATGTTCGGGAGATAGAAAATGGGCGACAAATTAATAAATCAACCTGCTAT
AAGATCAACGGTGCCGTTAAAATATTAAATATTAAATGGAGGGGCATCGT
TGATGGAGAATTATGCGTCGGCTGTGAATTGTCAGGTCCAGTCGAATGTG
GAACTGATGGAAAGTTTGGAAACAAAAATCTATTCAAGTTCCCTGACAGA
AGAGGCGTTCTTATCCCTACTGGTGAATTGAGAGATTCATGCGAATCAAC
CGCCCATTCAGAAATCGCTATCCTCCCTGGTAGAGGTTGGCCGCGGAAAG
AAACAACATATTTTATAGATCCTGATTGCATATGCTGCATTGAATTAACC
GAATTACTTCAACAACATCCAGATTTACGCATCAAAAGTAGAATAAAAAT
TCATAATATAGAAAATAAGCAAATTGGAGGGTTATGTGAATGGATTGGGT
TGATAAATGGACAGGAAGCACTTGGGATTACAATGGTAATTTCCGGATAT
GAATTGATTGAGTTAACAACGTTTTTAGGATAAAGATGTGAAAGGAACTA
ATGGAATCTTCAGTTGTTTTCGTATGTTTACATGCGACAAAGGAAAAGGA
GTTCTGGTGCTGGCGGATAATATAATTCACGGTATTTTGTATGTTTTGTT
TTATTGATAGCA
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protein sequence of SMED30024348-orf-1

>SMED30024348-orf-1 ID=SMED30024348-orf-1|Name=SMED30024348-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=277bp
IFLNTQRIKKDGMVSVKLLNGQANLKGTLKWHGSMNNVPVCAVELLQEHE
NGTNGRFWRTRLFECRDKLAIATLQQNLSPIKSELKFIKEINLNVVDPFN
VREIENGRQINKSTCYKINGAVKILNIKWRGIVDGELCVGCELSGPVECG
TDGKFGNKNLFKFPDRRGVLIPTGELRDSCESTAHSEIAILPGRGWPRKE
TTYFIDPDCICCIELTELLQQHPDLRIKSRIKIHNIENKQIGGLCEWIGL
INGQEALGITMVISGYELIELTTFLG*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000418head
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR036859CAP-Gly_dom_sf
IPR000938CAP-Gly_domain
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036859CAP Gly-rich domain superfamilyGENE3DG3DSA:2.30.30.190coord: 3..89
e-value: 3.0E-7
score: 31.8
IPR036859CAP Gly-rich domain superfamilySUPERFAMILYSSF74924Cap-Gly domaincoord: 4..73
IPR036859CAP Gly-rich domain superfamilySUPERFAMILYSSF74924Cap-Gly domaincoord: 113..176