Four and A half lim domains
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30024345 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of Four and A half lim domains vs. Ensembl Human
Match: FHL2 (four and a half LIM domains 2 [Source:HGNC Symbol;Acc:HGNC:3703]) HSP 1 Score: 340.502 bits (872), Expect = 2.512e-112 Identity = 150/273 (54.95%), Postives = 204/273 (74.73%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
F C C+ SL G +YIL+++ P C+ C+E+ +AN C++C IGCD KDLS+KDRHWHE CF C +C +SLVD+PFA K++ L C+DCY N+++++C C K G RK EY+G WHE CF+C C+QPIGTKSFIP++ CVPCYE+++A +C +C I GGVTY+ PWHKECF CT C KQL+G +FT++++ YC C+ +L+AKKC+ CT PI+G GG K+ISFE+R WH++CF C KC++SLVG+GFLT D I+CPDCG+
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGK 277 HSP 2 Score: 155.606 bits (392), Expect = 3.677e-42 Identity = 88/277 (31.77%), Postives = 133/277 (48.01%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGEL 1477
F C C+E L Y PYCV + L C C + I + + + H H A F C C NSL + K+DQ LC CY ++Y++ CQ+C I ++ + +K WHE CF C C + + F KDN +C CY+ + A +C C K G YR + WH+ECF+C CR+ + + F R+ C+ C+ + YA+KC C I G ++++ WH +CF C CS L G F ++ + C +C ++
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGKDI 279 HSP 3 Score: 115.931 bits (289), Expect = 3.259e-28 Identity = 66/219 (30.14%), Postives = 104/219 (47.49%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
WH CF C++C LVD + ++ C Y+ +C C + I TR ME + H F C C + +I KD+Q C+ CYE Q+A C QC I + ++++++ WH++CF C C L Q F +D+ YC +C+ + +A +C GC G + + +QWH +CF C C + + F+ DI+C C
Sbjct: 61 WHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPI--TTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 4 Score: 91.6633 bits (226), Expect = 8.020e-20 Identity = 57/181 (31.49%), Postives = 88/181 (48.62%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIG--CDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDC 1105
C +C+ +I G + + + SWH CF C C + + + N+ +CV Y + +C C + I T T EQ H F C +C L+G R+ +DD C+ C+ YA C C+ I +K +SF++R WH CF C +CS SLV + F T+ +++ C DC
Sbjct: 101 CQECKKTIMPGTRKMEYKGS-----SWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVT-YREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 5 Score: 54.299 bits (129), Expect = 2.881e-7 Identity = 36/124 (29.03%), Postives = 52/124 (41.94%), Query Frame = 2
Query: 560 ALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIF---TEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGC 922
A+ C QC+ I G + R + WH CF CT C + L + + YC+ + L +CA C I +Y E++ H F CK C SL G ++ + D LC C
Sbjct: 159 AMQCVQCKKPITTGGVTYR-------EQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. Ensembl Human
Match: FHL2 (four and a half LIM domains 2 [Source:HGNC Symbol;Acc:HGNC:3703]) HSP 1 Score: 340.502 bits (872), Expect = 2.512e-112 Identity = 150/273 (54.95%), Postives = 204/273 (74.73%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
F C C+ SL G +YIL+++ P C+ C+E+ +AN C++C IGCD KDLS+KDRHWHE CF C +C +SLVD+PFA K++ L C+DCY N+++++C C K G RK EY+G WHE CF+C C+QPIGTKSFIP++ CVPCYE+++A +C +C I GGVTY+ PWHKECF CT C KQL+G +FT++++ YC C+ +L+AKKC+ CT PI+G GG K+ISFE+R WH++CF C KC++SLVG+GFLT D I+CPDCG+
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGK 277 HSP 2 Score: 155.606 bits (392), Expect = 3.677e-42 Identity = 88/277 (31.77%), Postives = 133/277 (48.01%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGEL 1477
F C C+E L Y PYCV + L C C + I + + + H H A F C C NSL + K+DQ LC CY ++Y++ CQ+C I ++ + +K WHE CF C C + + F KDN +C CY+ + A +C C K G YR + WH+ECF+C CR+ + + F R+ C+ C+ + YA+KC C I G ++++ WH +CF C CS L G F ++ + C +C ++
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGKDI 279 HSP 3 Score: 115.931 bits (289), Expect = 3.259e-28 Identity = 66/219 (30.14%), Postives = 104/219 (47.49%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
WH CF C++C LVD + ++ C Y+ +C C + I TR ME + H F C C + +I KD+Q C+ CYE Q+A C QC I + ++++++ WH++CF C C L Q F +D+ YC +C+ + +A +C GC G + + +QWH +CF C C + + F+ DI+C C
Sbjct: 61 WHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPI--TTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 4 Score: 91.6633 bits (226), Expect = 8.020e-20 Identity = 57/181 (31.49%), Postives = 88/181 (48.62%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIG--CDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDC 1105
C +C+ +I G + + + SWH CF C C + + + N+ +CV Y + +C C + I T T EQ H F C +C L+G R+ +DD C+ C+ YA C C+ I +K +SF++R WH CF C +CS SLV + F T+ +++ C DC
Sbjct: 101 CQECKKTIMPGTRKMEYKGS-----SWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVT-YREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 5 Score: 54.299 bits (129), Expect = 2.881e-7 Identity = 36/124 (29.03%), Postives = 52/124 (41.94%), Query Frame = 2
Query: 560 ALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIF---TEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGC 922
A+ C QC+ I G + R + WH CF CT C + L + + YC+ + L +CA C I +Y E++ H F CK C SL G ++ + D LC C
Sbjct: 159 AMQCVQCKKPITTGGVTYR-------EQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. Ensembl Human
Match: FHL2 (four and a half LIM domains 2 [Source:HGNC Symbol;Acc:HGNC:3703]) HSP 1 Score: 340.502 bits (872), Expect = 2.512e-112 Identity = 150/273 (54.95%), Postives = 204/273 (74.73%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
F C C+ SL G +YIL+++ P C+ C+E+ +AN C++C IGCD KDLS+KDRHWHE CF C +C +SLVD+PFA K++ L C+DCY N+++++C C K G RK EY+G WHE CF+C C+QPIGTKSFIP++ CVPCYE+++A +C +C I GGVTY+ PWHKECF CT C KQL+G +FT++++ YC C+ +L+AKKC+ CT PI+G GG K+ISFE+R WH++CF C KC++SLVG+GFLT D I+CPDCG+
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGK 277 HSP 2 Score: 155.606 bits (392), Expect = 3.677e-42 Identity = 88/277 (31.77%), Postives = 133/277 (48.01%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGEL 1477
F C C+E L Y PYCV + L C C + I + + + H H A F C C NSL + K+DQ LC CY ++Y++ CQ+C I ++ + +K WHE CF C C + + F KDN +C CY+ + A +C C K G YR + WH+ECF+C CR+ + + F R+ C+ C+ + YA+KC C I G ++++ WH +CF C CS L G F ++ + C +C ++
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGKDI 279 HSP 3 Score: 115.931 bits (289), Expect = 3.259e-28 Identity = 66/219 (30.14%), Postives = 104/219 (47.49%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
WH CF C++C LVD + ++ C Y+ +C C + I TR ME + H F C C + +I KD+Q C+ CYE Q+A C QC I + ++++++ WH++CF C C L Q F +D+ YC +C+ + +A +C GC G + + +QWH +CF C C + + F+ DI+C C
Sbjct: 61 WHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPI--TTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 4 Score: 91.6633 bits (226), Expect = 8.020e-20 Identity = 57/181 (31.49%), Postives = 88/181 (48.62%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIG--CDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDC 1105
C +C+ +I G + + + SWH CF C C + + + N+ +CV Y + +C C + I T T EQ H F C +C L+G R+ +DD C+ C+ YA C C+ I +K +SF++R WH CF C +CS SLV + F T+ +++ C DC
Sbjct: 101 CQECKKTIMPGTRKMEYKGS-----SWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVT-YREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 5 Score: 54.299 bits (129), Expect = 2.881e-7 Identity = 36/124 (29.03%), Postives = 52/124 (41.94%), Query Frame = 2
Query: 560 ALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIF---TEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGC 922
A+ C QC+ I G + R + WH CF CT C + L + + YC+ + L +CA C I +Y E++ H F CK C SL G ++ + D LC C
Sbjct: 159 AMQCVQCKKPITTGGVTYR-------EQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. Ensembl Human
Match: FHL2 (four and a half LIM domains 2 [Source:HGNC Symbol;Acc:HGNC:3703]) HSP 1 Score: 340.502 bits (872), Expect = 2.512e-112 Identity = 150/273 (54.95%), Postives = 204/273 (74.73%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
F C C+ SL G +YIL+++ P C+ C+E+ +AN C++C IGCD KDLS+KDRHWHE CF C +C +SLVD+PFA K++ L C+DCY N+++++C C K G RK EY+G WHE CF+C C+QPIGTKSFIP++ CVPCYE+++A +C +C I GGVTY+ PWHKECF CT C KQL+G +FT++++ YC C+ +L+AKKC+ CT PI+G GG K+ISFE+R WH++CF C KC++SLVG+GFLT D I+CPDCG+
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGK 277 HSP 2 Score: 155.606 bits (392), Expect = 3.677e-42 Identity = 88/277 (31.77%), Postives = 133/277 (48.01%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGEL 1477
F C C+E L Y PYCV + L C C + I + + + H H A F C C NSL + K+DQ LC CY ++Y++ CQ+C I ++ + +K WHE CF C C + + F KDN +C CY+ + A +C C K G YR + WH+ECF+C CR+ + + F R+ C+ C+ + YA+KC C I G ++++ WH +CF C CS L G F ++ + C +C ++
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGKDI 279 HSP 3 Score: 115.931 bits (289), Expect = 3.259e-28 Identity = 66/219 (30.14%), Postives = 104/219 (47.49%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
WH CF C++C LVD + ++ C Y+ +C C + I TR ME + H F C C + +I KD+Q C+ CYE Q+A C QC I + ++++++ WH++CF C C L Q F +D+ YC +C+ + +A +C GC G + + +QWH +CF C C + + F+ DI+C C
Sbjct: 61 WHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPI--TTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 4 Score: 91.6633 bits (226), Expect = 8.020e-20 Identity = 57/181 (31.49%), Postives = 88/181 (48.62%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIG--CDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDC 1105
C +C+ +I G + + + SWH CF C C + + + N+ +CV Y + +C C + I T T EQ H F C +C L+G R+ +DD C+ C+ YA C C+ I +K +SF++R WH CF C +CS SLV + F T+ +++ C DC
Sbjct: 101 CQECKKTIMPGTRKMEYKGS-----SWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVT-YREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 5 Score: 54.299 bits (129), Expect = 2.881e-7 Identity = 36/124 (29.03%), Postives = 52/124 (41.94%), Query Frame = 2
Query: 560 ALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIF---TEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGC 922
A+ C QC+ I G + R + WH CF CT C + L + + YC+ + L +CA C I +Y E++ H F CK C SL G ++ + D LC C
Sbjct: 159 AMQCVQCKKPITTGGVTYR-------EQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. Ensembl Human
Match: FHL2 (four and a half LIM domains 2 [Source:HGNC Symbol;Acc:HGNC:3703]) HSP 1 Score: 340.502 bits (872), Expect = 2.512e-112 Identity = 150/273 (54.95%), Postives = 204/273 (74.73%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
F C C+ SL G +YIL+++ P C+ C+E+ +AN C++C IGCD KDLS+KDRHWHE CF C +C +SLVD+PFA K++ L C+DCY N+++++C C K G RK EY+G WHE CF+C C+QPIGTKSFIP++ CVPCYE+++A +C +C I GGVTY+ PWHKECF CT C KQL+G +FT++++ YC C+ +L+AKKC+ CT PI+G GG K+ISFE+R WH++CF C KC++SLVG+GFLT D I+CPDCG+
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGK 277 HSP 2 Score: 155.606 bits (392), Expect = 3.677e-42 Identity = 88/277 (31.77%), Postives = 133/277 (48.01%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGEL 1477
F C C+E L Y PYCV + L C C + I + + + H H A F C C NSL + K+DQ LC CY ++Y++ CQ+C I ++ + +K WHE CF C C + + F KDN +C CY+ + A +C C K G YR + WH+ECF+C CR+ + + F R+ C+ C+ + YA+KC C I G ++++ WH +CF C CS L G F ++ + C +C ++
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGKDI 279 HSP 3 Score: 115.931 bits (289), Expect = 3.259e-28 Identity = 66/219 (30.14%), Postives = 104/219 (47.49%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
WH CF C++C LVD + ++ C Y+ +C C + I TR ME + H F C C + +I KD+Q C+ CYE Q+A C QC I + ++++++ WH++CF C C L Q F +D+ YC +C+ + +A +C GC G + + +QWH +CF C C + + F+ DI+C C
Sbjct: 61 WHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPI--TTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 4 Score: 91.6633 bits (226), Expect = 8.020e-20 Identity = 57/181 (31.49%), Postives = 88/181 (48.62%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIG--CDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDC 1105
C +C+ +I G + + + SWH CF C C + + + N+ +CV Y + +C C + I T T EQ H F C +C L+G R+ +DD C+ C+ YA C C+ I +K +SF++R WH CF C +CS SLV + F T+ +++ C DC
Sbjct: 101 CQECKKTIMPGTRKMEYKGS-----SWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVT-YREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 5 Score: 54.299 bits (129), Expect = 2.881e-7 Identity = 36/124 (29.03%), Postives = 52/124 (41.94%), Query Frame = 2
Query: 560 ALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIF---TEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGC 922
A+ C QC+ I G + R + WH CF CT C + L + + YC+ + L +CA C I +Y E++ H F CK C SL G ++ + D LC C
Sbjct: 159 AMQCVQCKKPITTGGVTYR-------EQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. Ensembl Celegans
Match: lim-9 (LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5EDJ5]) HSP 1 Score: 549.666 bits (1415), Expect = 0.000e+0 Identity = 255/516 (49.42%), Postives = 347/516 (67.25%), Query Frame = 2
Query: 131 GFKIHGWRKTCLGCDCSVDRHDITDESPLPILEEMKL---ADKPYYNLFMESLKMARKYGLSWLPIGLQASEVEQFTSIVGGNIASRA---GEKLRKQKLRRQIPVHDRCVEKFSHLETPTERQEAEKFLQFRNNQDLGVGHVYRLDMAALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
G+K H WRK C+ C H++ L + + + A P + + + +G +W+P GL +VE++ S + N+ R GEKLR+++L Q+P D V HL + TER+ E+F+ RN L +G+V A+ C +C ++ ++AV ++ WHP CF C C+++LVD YC N+ YC RHYA+L +PRC+ CDELIF EYT+AM ++ H HF C CD +LTG RYI++D+QP CI CYE +AN C +C+ IG DSKDLS+KD+HWHE CF C C SLVD PF +K++ ++CS+CYD FATRCDGC+++F+AGM+K EY+GKQWH++CF C C+ IGTKSFIP+ D+ C PCYEEK+A +C+KC VI GGVTYK PWH+ECF CT+C+ LAG +FTSK+EKPYCA CYG+LFAK+C+ CTKPITG GG KFISFEDRHWH++CF C +C SLVGKGF+T+ +I+CP+C +
Sbjct: 271 GWKQHPWRKVCVHCKSDRSDHELPPNQALNVYNRLGIQPPAGMPQSGVAEPEVPGSVGHGYAWVPPGLSRKKVEEYMSQLPNNVVPRTNSNGEKLREKQLLLQLPRQDLSVAYCRHLTSQTERKVYEEFVNARNEIALDIGYVSSNINKAMECHKCSGILETNEMAVIAPKLGDS-TGWHPACFTCQACEQLLVDLTYCVKDNQIYCERHYAELHKPRCSACDELIFAGEYTKAMNKDWHSDHFCCWQCDQTLTGQRYIMRDEQPYCIKCYEDVFANQCDECAKPIGIDSKDLSYKDKHWHEHCFLCSMCKISLVDMPFGSKNDRIFCSNCYDQAFATRCDGCNEIFRAGMKKMEYKGKQWHDKCFCCAHCKLAIGTKSFIPKNDDVFCGPCYEEKFATRCSKCKKVITAGGVTYKNEPWHRECFCCTNCNSSLAGQRFTSKDEKPYCANCYGDLFAKRCNACTKPITGIGGAKFISFEDRHWHNDCFICAQCTTSLVGKGFITDGHEILCPECAK 785
BLAST of Four and A half lim domains vs. Ensembl Celegans
Match: lim-9 (LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5EDJ5]) HSP 1 Score: 548.895 bits (1413), Expect = 0.000e+0 Identity = 255/516 (49.42%), Postives = 347/516 (67.25%), Query Frame = 2
Query: 131 GFKIHGWRKTCLGCDCSVDRHDITDESPLPILEEMKL---ADKPYYNLFMESLKMARKYGLSWLPIGLQASEVEQFTSIVGGNIASRA---GEKLRKQKLRRQIPVHDRCVEKFSHLETPTERQEAEKFLQFRNNQDLGVGHVYRLDMAALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
G+K H WRK C+ C H++ L + + + A P + + + +G +W+P GL +VE++ S + N+ R GEKLR+++L Q+P D V HL + TER+ E+F+ RN L +G+V A+ C +C ++ ++AV ++ WHP CF C C+++LVD YC N+ YC RHYA+L +PRC+ CDELIF EYT+AM ++ H HF C CD +LTG RYI++D+QP CI CYE +AN C +C+ IG DSKDLS+KD+HWHE CF C C SLVD PF +K++ ++CS+CYD FATRCDGC+++F+AGM+K EY+GKQWH++CF C C+ IGTKSFIP+ D+ C PCYEEK+A +C+KC VI GGVTYK PWH+ECF CT+C+ LAG +FTSK+EKPYCA CYG+LFAK+C+ CTKPITG GG KFISFEDRHWH++CF C +C SLVGKGF+T+ +I+CP+C +
Sbjct: 241 GWKQHPWRKVCVHCKSDRSDHELPPNQALNVYNRLGIQPPAGMPQSGVAEPEVPGSVGHGYAWVPPGLSRKKVEEYMSQLPNNVVPRTNSNGEKLREKQLLLQLPRQDLSVAYCRHLTSQTERKVYEEFVNARNEIALDIGYVSSNINKAMECHKCSGILETNEMAVIAPKLGDS-TGWHPACFTCQACEQLLVDLTYCVKDNQIYCERHYAELHKPRCSACDELIFAGEYTKAMNKDWHSDHFCCWQCDQTLTGQRYIMRDEQPYCIKCYEDVFANQCDECAKPIGIDSKDLSYKDKHWHEHCFLCSMCKISLVDMPFGSKNDRIFCSNCYDQAFATRCDGCNEIFRAGMKKMEYKGKQWHDKCFCCAHCKLAIGTKSFIPKNDDVFCGPCYEEKFATRCSKCKKVITAGGVTYKNEPWHRECFCCTNCNSSLAGQRFTSKDEKPYCANCYGDLFAKRCNACTKPITGIGGAKFISFEDRHWHNDCFICAQCTTSLVGKGFITDGHEILCPECAK 755
BLAST of Four and A half lim domains vs. Ensembl Celegans
Match: lim-9 (LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5EDJ5]) HSP 1 Score: 547.354 bits (1409), Expect = 0.000e+0 Identity = 255/516 (49.42%), Postives = 347/516 (67.25%), Query Frame = 2
Query: 131 GFKIHGWRKTCLGCDCSVDRHDITDESPLPILEEMKL---ADKPYYNLFMESLKMARKYGLSWLPIGLQASEVEQFTSIVGGNIASRA---GEKLRKQKLRRQIPVHDRCVEKFSHLETPTERQEAEKFLQFRNNQDLGVGHVYRLDMAALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
G+K H WRK C+ C H++ L + + + A P + + + +G +W+P GL +VE++ S + N+ R GEKLR+++L Q+P D V HL + TER+ E+F+ RN L +G+V A+ C +C ++ ++AV ++ WHP CF C C+++LVD YC N+ YC RHYA+L +PRC+ CDELIF EYT+AM ++ H HF C CD +LTG RYI++D+QP CI CYE +AN C +C+ IG DSKDLS+KD+HWHE CF C C SLVD PF +K++ ++CS+CYD FATRCDGC+++F+AGM+K EY+GKQWH++CF C C+ IGTKSFIP+ D+ C PCYEEK+A +C+KC VI GGVTYK PWH+ECF CT+C+ LAG +FTSK+EKPYCA CYG+LFAK+C+ CTKPITG GG KFISFEDRHWH++CF C +C SLVGKGF+T+ +I+CP+C +
Sbjct: 134 GWKQHPWRKVCVHCKSDRSDHELPPNQALNVYNRLGIQPPAGMPQSGVAEPEVPGSVGHGYAWVPPGLSRKKVEEYMSQLPNNVVPRTNSNGEKLREKQLLLQLPRQDLSVAYCRHLTSQTERKVYEEFVNARNEIALDIGYVSSNINKAMECHKCSGILETNEMAVIAPKLGDS-TGWHPACFTCQACEQLLVDLTYCVKDNQIYCERHYAELHKPRCSACDELIFAGEYTKAMNKDWHSDHFCCWQCDQTLTGQRYIMRDEQPYCIKCYEDVFANQCDECAKPIGIDSKDLSYKDKHWHEHCFLCSMCKISLVDMPFGSKNDRIFCSNCYDQAFATRCDGCNEIFRAGMKKMEYKGKQWHDKCFCCAHCKLAIGTKSFIPKNDDVFCGPCYEEKFATRCSKCKKVITAGGVTYKNEPWHRECFCCTNCNSSLAGQRFTSKDEKPYCANCYGDLFAKRCNACTKPITGIGGAKFISFEDRHWHNDCFICAQCTTSLVGKGFITDGHEILCPECAK 648
BLAST of Four and A half lim domains vs. Ensembl Celegans
Match: lim-9 (LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5EDJ5]) HSP 1 Score: 545.814 bits (1405), Expect = 0.000e+0 Identity = 255/516 (49.42%), Postives = 347/516 (67.25%), Query Frame = 2
Query: 131 GFKIHGWRKTCLGCDCSVDRHDITDESPLPILEEMKL---ADKPYYNLFMESLKMARKYGLSWLPIGLQASEVEQFTSIVGGNIASRA---GEKLRKQKLRRQIPVHDRCVEKFSHLETPTERQEAEKFLQFRNNQDLGVGHVYRLDMAALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
G+K H WRK C+ C H++ L + + + A P + + + +G +W+P GL +VE++ S + N+ R GEKLR+++L Q+P D V HL + TER+ E+F+ RN L +G+V A+ C +C ++ ++AV ++ WHP CF C C+++LVD YC N+ YC RHYA+L +PRC+ CDELIF EYT+AM ++ H HF C CD +LTG RYI++D+QP CI CYE +AN C +C+ IG DSKDLS+KD+HWHE CF C C SLVD PF +K++ ++CS+CYD FATRCDGC+++F+AGM+K EY+GKQWH++CF C C+ IGTKSFIP+ D+ C PCYEEK+A +C+KC VI GGVTYK PWH+ECF CT+C+ LAG +FTSK+EKPYCA CYG+LFAK+C+ CTKPITG GG KFISFEDRHWH++CF C +C SLVGKGF+T+ +I+CP+C +
Sbjct: 42 GWKQHPWRKVCVHCKSDRSDHELPPNQALNVYNRLGIQPPAGMPQSGVAEPEVPGSVGHGYAWVPPGLSRKKVEEYMSQLPNNVVPRTNSNGEKLREKQLLLQLPRQDLSVAYCRHLTSQTERKVYEEFVNARNEIALDIGYVSSNINKAMECHKCSGILETNEMAVIAPKLGDS-TGWHPACFTCQACEQLLVDLTYCVKDNQIYCERHYAELHKPRCSACDELIFAGEYTKAMNKDWHSDHFCCWQCDQTLTGQRYIMRDEQPYCIKCYEDVFANQCDECAKPIGIDSKDLSYKDKHWHEHCFLCSMCKISLVDMPFGSKNDRIFCSNCYDQAFATRCDGCNEIFRAGMKKMEYKGKQWHDKCFCCAHCKLAIGTKSFIPKNDDVFCGPCYEEKFATRCSKCKKVITAGGVTYKNEPWHRECFCCTNCNSSLAGQRFTSKDEKPYCANCYGDLFAKRCNACTKPITGIGGAKFISFEDRHWHNDCFICAQCTTSLVGKGFITDGHEILCPECAK 556
BLAST of Four and A half lim domains vs. Ensembl Celegans
Match: lim-9 (LIM domain family [Source:UniProtKB/TrEMBL;Acc:G5EDJ5]) HSP 1 Score: 545.814 bits (1405), Expect = 0.000e+0 Identity = 255/516 (49.42%), Postives = 347/516 (67.25%), Query Frame = 2
Query: 131 GFKIHGWRKTCLGCDCSVDRHDITDESPLPILEEMKL---ADKPYYNLFMESLKMARKYGLSWLPIGLQASEVEQFTSIVGGNIASRA---GEKLRKQKLRRQIPVHDRCVEKFSHLETPTERQEAEKFLQFRNNQDLGVGHVYRLDMAALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
G+K H WRK C+ C H++ L + + + A P + + + +G +W+P GL +VE++ S + N+ R GEKLR+++L Q+P D V HL + TER+ E+F+ RN L +G+V A+ C +C ++ ++AV ++ WHP CF C C+++LVD YC N+ YC RHYA+L +PRC+ CDELIF EYT+AM ++ H HF C CD +LTG RYI++D+QP CI CYE +AN C +C+ IG DSKDLS+KD+HWHE CF C C SLVD PF +K++ ++CS+CYD FATRCDGC+++F+AGM+K EY+GKQWH++CF C C+ IGTKSFIP+ D+ C PCYEEK+A +C+KC VI GGVTYK PWH+ECF CT+C+ LAG +FTSK+EKPYCA CYG+LFAK+C+ CTKPITG GG KFISFEDRHWH++CF C +C SLVGKGF+T+ +I+CP+C +
Sbjct: 42 GWKQHPWRKVCVHCKSDRSDHELPPNQALNVYNRLGIQPPAGMPQSGVAEPEVPGSVGHGYAWVPPGLSRKKVEEYMSQLPNNVVPRTNSNGEKLREKQLLLQLPRQDLSVAYCRHLTSQTERKVYEEFVNARNEIALDIGYVSSNINKAMECHKCSGILETNEMAVIAPKLGDS-TGWHPACFTCQACEQLLVDLTYCVKDNQIYCERHYAELHKPRCSACDELIFAGEYTKAMNKDWHSDHFCCWQCDQTLTGQRYIMRDEQPYCIKCYEDVFANQCDECAKPIGIDSKDLSYKDKHWHEHCFLCSMCKISLVDMPFGSKNDRIFCSNCYDQAFATRCDGCNEIFRAGMKKMEYKGKQWHDKCFCCAHCKLAIGTKSFIPKNDDVFCGPCYEEKFATRCSKCKKVITAGGVTYKNEPWHRECFCCTNCNSSLAGQRFTSKDEKPYCANCYGDLFAKRCNACTKPITGIGGAKFISFEDRHWHNDCFICAQCTTSLVGKGFITDGHEILCPECAK 556
BLAST of Four and A half lim domains vs. Ensembl Fly
Match: Lmpt (gene:FBgn0261565 transcript:FBtr0302779) HSP 1 Score: 528.48 bits (1360), Expect = 0.000e+0 Identity = 256/526 (48.67%), Postives = 341/526 (64.83%), Query Frame = 2
Query: 95 GTPCLDCVDKCRGFKIHGWRKTCLGCDCSVDRHDITDESPLPILEEMKLADKPYYNLFMESLKMARKYGLSWLPIGLQASE-VEQFTSIVGGNIASRAGEK---LRKQKLRRQIPVHDRCVEKFSHLETPTERQEAEKFLQFRNNQDLGVGHVYRLDMAALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
G C C C GF H WRKTC C C + H I + + E + + +E AR G +W+P G++AS + ++ + + R G + R++++ Q+P D +E HLE E E F+ RN L + ++ C C + I AG+L V + M WHP+CF C+ C+ +LVD YC + +K YC RHYA++++PRCA CDELIF+ EYT+AM+++ H HF C CD SLTG RY+++DD P CI CYE+ +AN C++C+ IG DSKDLS+KD+HWHE CF CF+C SLVD+ F K + +YC +CYD +FA+RCDGC +VF+AG +K EY+ +QWHE CF C C+ IGTKSFIPREQ+I C CYEEK+A +C KC VI GGVTYK PWH+ECFTCTHC+ LAG +FTS++EKPYCAEC+GELFAK+C+ C KPITG GG +FISFEDRHWH +CF C C SLVG+GF+T+ I+CPDC +
Sbjct: 37 GVRCEQCKSDCPGFAAHDWRKTCQSCKCPREAHAIYQQQTTNVHERLGFKLVSPADSGVE----ARDLGFTWVPPGVRASSRINRYFEQLPDEMVPRLGSEGACSRERQISYQLPKQDLSLEHCKHLEVQHE-ASFEDFVTARNEIALDIAYIKDAPYDE-HCAHCDNEIAAGELVVAAPKFVESVM-WHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAK 555 HSP 2 Score: 122.094 bits (305), Expect = 6.679e-29 Identity = 70/223 (31.39%), Postives = 110/223 (49.33%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAME---QEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKA--GMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEK 1300
WH CF C +C LVD + ++K YC Y RC C E+ T+ ME ++ H F C C ++ +I ++ + C GCYE ++A C +C+ I S +++K+ WH +CF C C+ +L Q F ++D YC++C+ FA RC C K G R + + WH +CF+C C+ + + FI DI+C C ++K
Sbjct: 339 WHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVF--RAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVI--TSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQK 557 HSP 3 Score: 87.4261 bits (215), Expect = 1.017e-17 Identity = 50/160 (31.25%), Postives = 74/160 (46.25%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGC--DSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKF 1120
WH CF C C + + + YC Y + RC C+++I + T E H F C C+ +L G R+ +D++P C C+ +A C C I ++ +SF+DRHWH CF C C +SLV + F T ++ C DC K
Sbjct: 400 WHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHREC-FTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQKL 558
BLAST of Four and A half lim domains vs. Ensembl Fly
Match: Lmpt (gene:FBgn0261565 transcript:FBtr0302781) HSP 1 Score: 528.48 bits (1360), Expect = 0.000e+0 Identity = 256/526 (48.67%), Postives = 341/526 (64.83%), Query Frame = 2
Query: 95 GTPCLDCVDKCRGFKIHGWRKTCLGCDCSVDRHDITDESPLPILEEMKLADKPYYNLFMESLKMARKYGLSWLPIGLQASE-VEQFTSIVGGNIASRAGEK---LRKQKLRRQIPVHDRCVEKFSHLETPTERQEAEKFLQFRNNQDLGVGHVYRLDMAALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
G C C C GF H WRKTC C C + H I + + E + + +E AR G +W+P G++AS + ++ + + R G + R++++ Q+P D +E HLE E E F+ RN L + ++ C C + I AG+L V + M WHP+CF C+ C+ +LVD YC + +K YC RHYA++++PRCA CDELIF+ EYT+AM+++ H HF C CD SLTG RY+++DD P CI CYE+ +AN C++C+ IG DSKDLS+KD+HWHE CF CF+C SLVD+ F K + +YC +CYD +FA+RCDGC +VF+AG +K EY+ +QWHE CF C C+ IGTKSFIPREQ+I C CYEEK+A +C KC VI GGVTYK PWH+ECFTCTHC+ LAG +FTS++EKPYCAEC+GELFAK+C+ C KPITG GG +FISFEDRHWH +CF C C SLVG+GF+T+ I+CPDC +
Sbjct: 37 GVRCEQCKSDCPGFAAHDWRKTCQSCKCPREAHAIYQQQTTNVHERLGFKLVSPADSGVE----ARDLGFTWVPPGVRASSRINRYFEQLPDEMVPRLGSEGACSRERQISYQLPKQDLSLEHCKHLEVQHE-ASFEDFVTARNEIALDIAYIKDAPYDE-HCAHCDNEIAAGELVVAAPKFVESVM-WHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAK 555 HSP 2 Score: 122.094 bits (305), Expect = 6.679e-29 Identity = 70/223 (31.39%), Postives = 110/223 (49.33%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAME---QEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKA--GMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEK 1300
WH CF C +C LVD + ++K YC Y RC C E+ T+ ME ++ H F C C ++ +I ++ + C GCYE ++A C +C+ I S +++K+ WH +CF C C+ +L Q F ++D YC++C+ FA RC C K G R + + WH +CF+C C+ + + FI DI+C C ++K
Sbjct: 339 WHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVF--RAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVI--TSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQK 557 HSP 3 Score: 87.4261 bits (215), Expect = 1.017e-17 Identity = 50/160 (31.25%), Postives = 74/160 (46.25%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGC--DSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKF 1120
WH CF C C + + + YC Y + RC C+++I + T E H F C C+ +L G R+ +D++P C C+ +A C C I ++ +SF+DRHWH CF C C +SLV + F T ++ C DC K
Sbjct: 400 WHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHREC-FTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQKL 558
BLAST of Four and A half lim domains vs. Ensembl Fly
Match: Lmpt (gene:FBgn0261565 transcript:FBtr0306704) HSP 1 Score: 528.48 bits (1360), Expect = 0.000e+0 Identity = 256/526 (48.67%), Postives = 341/526 (64.83%), Query Frame = 2
Query: 95 GTPCLDCVDKCRGFKIHGWRKTCLGCDCSVDRHDITDESPLPILEEMKLADKPYYNLFMESLKMARKYGLSWLPIGLQASE-VEQFTSIVGGNIASRAGEK---LRKQKLRRQIPVHDRCVEKFSHLETPTERQEAEKFLQFRNNQDLGVGHVYRLDMAALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
G C C C GF H WRKTC C C + H I + + E + + +E AR G +W+P G++AS + ++ + + R G + R++++ Q+P D +E HLE E E F+ RN L + ++ C C + I AG+L V + M WHP+CF C+ C+ +LVD YC + +K YC RHYA++++PRCA CDELIF+ EYT+AM+++ H HF C CD SLTG RY+++DD P CI CYE+ +AN C++C+ IG DSKDLS+KD+HWHE CF CF+C SLVD+ F K + +YC +CYD +FA+RCDGC +VF+AG +K EY+ +QWHE CF C C+ IGTKSFIPREQ+I C CYEEK+A +C KC VI GGVTYK PWH+ECFTCTHC+ LAG +FTS++EKPYCAEC+GELFAK+C+ C KPITG GG +FISFEDRHWH +CF C C SLVG+GF+T+ I+CPDC +
Sbjct: 37 GVRCEQCKSDCPGFAAHDWRKTCQSCKCPREAHAIYQQQTTNVHERLGFKLVSPADSGVE----ARDLGFTWVPPGVRASSRINRYFEQLPDEMVPRLGSEGACSRERQISYQLPKQDLSLEHCKHLEVQHE-ASFEDFVTARNEIALDIAYIKDAPYDE-HCAHCDNEIAAGELVVAAPKFVESVM-WHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAK 555 HSP 2 Score: 122.094 bits (305), Expect = 6.679e-29 Identity = 70/223 (31.39%), Postives = 110/223 (49.33%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAME---QEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKA--GMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEK 1300
WH CF C +C LVD + ++K YC Y RC C E+ T+ ME ++ H F C C ++ +I ++ + C GCYE ++A C +C+ I S +++K+ WH +CF C C+ +L Q F ++D YC++C+ FA RC C K G R + + WH +CF+C C+ + + FI DI+C C ++K
Sbjct: 339 WHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVF--RAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVI--TSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQK 557 HSP 3 Score: 87.4261 bits (215), Expect = 1.017e-17 Identity = 50/160 (31.25%), Postives = 74/160 (46.25%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGC--DSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKF 1120
WH CF C C + + + YC Y + RC C+++I + T E H F C C+ +L G R+ +D++P C C+ +A C C I ++ +SF+DRHWH CF C C +SLV + F T ++ C DC K
Sbjct: 400 WHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHREC-FTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQKL 558
BLAST of Four and A half lim domains vs. Ensembl Fly
Match: Lmpt (gene:FBgn0261565 transcript:FBtr0332703) HSP 1 Score: 513.457 bits (1321), Expect = 1.663e-176 Identity = 248/507 (48.92%), Postives = 333/507 (65.68%), Query Frame = 2
Query: 152 RKTCLGCDCSVDRHDITDESPLPILEEMKLADKPYYNLFMESLKMARKYGLSWLPIGLQASE-VEQFTSIVGGNIASRAGEK---LRKQKLRRQIPVHDRCVEKFSHLETPTERQEAEKFLQFRNNQDLGVGHVYRLDMAALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
RKTC C C + H I + + E + + +E AR G +W+P G++AS + ++ + + R G + R++++ Q+P D +E HLE E E F+ RN L + ++ C C + I AG+L V + M WHP+CF C+ C+ +LVD YC + +K YC RHYA++++PRCA CDELIF+ EYT+AM+++ H HF C CD SLTG RY+++DD P CI CYE+ +AN C++C+ IG DSKDLS+KD+HWHE CF CF+C SLVD+ F K + +YC +CYD +FA+RCDGC +VF+AG +K EY+ +QWHE CF C C+ IGTKSFIPREQ+I C CYEEK+A +C KC VI GGVTYK PWH+ECFTCTHC+ LAG +FTS++EKPYCAEC+GELFAK+C+ C KPITG GG +FISFEDRHWH +CF C C SLVG+GF+T+ I+CPDC +
Sbjct: 26 RKTCQSCKCPREAHAIYQQQTTNVHERLGFKLVSPADSGVE----ARDLGFTWVPPGVRASSRINRYFEQLPDEMVPRLGSEGACSRERQISYQLPKQDLSLEHCKHLEVQHE-ASFEDFVTARNEIALDIAYIKDAPYDE-HCAHCDNEIAAGELVVAAPKFVESVM-WHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAK 525 HSP 2 Score: 122.479 bits (306), Expect = 3.928e-29 Identity = 70/223 (31.39%), Postives = 110/223 (49.33%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAME---QEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKA--GMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEK 1300
WH CF C +C LVD + ++K YC Y RC C E+ T+ ME ++ H F C C ++ +I ++ + C GCYE ++A C +C+ I S +++K+ WH +CF C C+ +L Q F ++D YC++C+ FA RC C K G R + + WH +CF+C C+ + + FI DI+C C ++K
Sbjct: 309 WHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVF--RAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVI--TSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQK 527 HSP 3 Score: 87.8113 bits (216), Expect = 8.139e-18 Identity = 50/160 (31.25%), Postives = 74/160 (46.25%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGC--DSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKF 1120
WH CF C C + + + YC Y + RC C+++I + T E H F C C+ +L G R+ +D++P C C+ +A C C I ++ +SF+DRHWH CF C C +SLV + F T ++ C DC K
Sbjct: 370 WHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHREC-FTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQKL 528
BLAST of Four and A half lim domains vs. Ensembl Fly
Match: Lmpt (gene:FBgn0261565 transcript:FBtr0302780) HSP 1 Score: 513.457 bits (1321), Expect = 4.140e-176 Identity = 248/507 (48.92%), Postives = 333/507 (65.68%), Query Frame = 2
Query: 152 RKTCLGCDCSVDRHDITDESPLPILEEMKLADKPYYNLFMESLKMARKYGLSWLPIGLQASE-VEQFTSIVGGNIASRAGEK---LRKQKLRRQIPVHDRCVEKFSHLETPTERQEAEKFLQFRNNQDLGVGHVYRLDMAALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
RKTC C C + H I + + E + + +E AR G +W+P G++AS + ++ + + R G + R++++ Q+P D +E HLE E E F+ RN L + ++ C C + I AG+L V + M WHP+CF C+ C+ +LVD YC + +K YC RHYA++++PRCA CDELIF+ EYT+AM+++ H HF C CD SLTG RY+++DD P CI CYE+ +AN C++C+ IG DSKDLS+KD+HWHE CF CF+C SLVD+ F K + +YC +CYD +FA+RCDGC +VF+AG +K EY+ +QWHE CF C C+ IGTKSFIPREQ+I C CYEEK+A +C KC VI GGVTYK PWH+ECFTCTHC+ LAG +FTS++EKPYCAEC+GELFAK+C+ C KPITG GG +FISFEDRHWH +CF C C SLVG+GF+T+ I+CPDC +
Sbjct: 55 RKTCQSCKCPREAHAIYQQQTTNVHERLGFKLVSPADSGVE----ARDLGFTWVPPGVRASSRINRYFEQLPDEMVPRLGSEGACSRERQISYQLPKQDLSLEHCKHLEVQHE-ASFEDFVTARNEIALDIAYIKDAPYDE-HCAHCDNEIAAGELVVAAPKFVESVM-WHPKCFTCSTCNSLLVDLTYCVHDDKVYCERHYAEMLKPRCAGCDELIFSGEYTKAMDKDWHSGHFCCWQCDESLTGQRYVIRDDHPYCIKCYENVFANTCEECNKIIGIDSKDLSYKDKHWHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVFRAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAK 554 HSP 2 Score: 122.479 bits (306), Expect = 5.732e-29 Identity = 70/223 (31.39%), Postives = 110/223 (49.33%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAME---QEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKA--GMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEK 1300
WH CF C +C LVD + ++K YC Y RC C E+ T+ ME ++ H F C C ++ +I ++ + C GCYE ++A C +C+ I S +++K+ WH +CF C C+ +L Q F ++D YC++C+ FA RC C K G R + + WH +CF+C C+ + + FI DI+C C ++K
Sbjct: 338 WHEACFLCFKCHLSLVDKQFGAKADKIYCGNCYDAQFASRCDGCGEVF--RAGTKKMEYKTRQWHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVIT--SGGVTYKNEPWHRECFTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQK 556 HSP 3 Score: 87.4261 bits (215), Expect = 1.137e-17 Identity = 50/160 (31.25%), Postives = 74/160 (46.25%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGC--DSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKF 1120
WH CF C C + + + YC Y + RC C+++I + T E H F C C+ +L G R+ +D++P C C+ +A C C I ++ +SF+DRHWH CF C C +SLV + F T ++ C DC K
Sbjct: 399 WHENCFCCCVCKTAIGTKSFIPREQEIYCAGCYEEKFATRCIKCNKVITSGGVTYKNEPWHREC-FTCTHCNITLAGQRFTSRDEKPYCAECFGELFAKRCTACVKPITGIGGTRFISFEDRHWHHDCFVCASCKASLVGRGFITDGPDILCPDCAKQKL 557
BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Match: fhl5 (four and a half LIM domains 5 [Source:ZFIN;Acc:ZDB-GENE-040822-3]) HSP 1 Score: 338.576 bits (867), Expect = 6.930e-112 Identity = 154/273 (56.41%), Postives = 198/273 (72.53%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
F C C ++L G +YILK+D C CYE+ +AN C+ CS IGC+ KDLS+KDRHWHE CFKC +CS SLVD+PFA KD + C++CY ++++++C C K G RK EY+G WHE CFLC C+QPIGTKSFIP++ + CVPC+E+++A +C C I GGVTY PWH+ECFTC C +QLAG +FTS+E PYC +C+ L+AKKC CTK IT G K+ISFE+R WHSECFTC +C++SLVG+GFLT D I+C DCGR
Sbjct: 6 FDCHYCKDTLLGKKYILKEDTQYCTKCYENLFANCCEVCSLPIGCNCKDLSYKDRHWHENCFKCAKCSRSLVDKPFAAKDELMLCTECYSHEYSSKCSTCKKTVMPGSRKMEYKGNSWHETCFLCQRCQQPIGTKSFIPKDNNYFCVPCFEKQFAYQCCACKKAITTGGVTYHDKPWHRECFTCIGCKRQLAGQRFTSRENYPYCLDCFSNLYAKKCVGCTKAITSLAGAKYISFEERQWHSECFTCMQCSVSLVGRGFLTQRDDILCTDCGR 278 HSP 2 Score: 148.673 bits (374), Expect = 6.375e-40 Identity = 84/273 (30.77%), Postives = 122/273 (44.69%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAEC 1465
F C C + L+ Y + YC + Y L C C I + + H H F C C SL + KD+ LC CY +Y++ C C + S+ + +K WHE CF C C + + F KDNN +C C++ +FA +C C K G Y K WH ECF C+ C++ + + F RE C+ C+ YA+KC C I ++++ WH ECFTC CS L G F ++ + C +C
Sbjct: 6 FDCHYCKDTLLGKKYILKEDTQYCTKCYENLFANCCEVCSLPIGCNCKDLSYKDRHWHENCFKCAKCSRSLVDKPFAAKDELMLCTECYSHEYSSKCSTCKKTVMPGSRKMEYKGNSWHETCFLCQRCQQPIGTKSFIPKDNNYFCVPCFEKQFAYQCCACKKAITTG--GVTYHDKPWHRECFTCIGCKRQLAGQRFTSRENYPYCLDCFSNLYAKKCVGCTKAITSLAGAKYISFEERQWHSECFTCMQCSVSLVGRGFLTQRDDILCTDC 276 HSP 3 Score: 134.42 bits (337), Expect = 8.296e-35 Identity = 79/273 (28.94%), Postives = 125/273 (45.79%), Query Frame = 2
Query: 518 QDLGVGHVYRLDMAALPCTQCQSSIQAG-------QLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEK 1300
+D +G Y L CT+C ++ A + + + WH CFKC +C LVD + C Y+ +C+TC + + +R ME + + H F C+ C + +I KD+ C+ C+E Q+A C C I + +++ D+ WH +CF C C L Q F +++N YC DC+ N +A +C GC K AG + + +QWH ECF C+ C + + F+ + DI+C C EK
Sbjct: 12 KDTLLGKKYILKEDTQYCTKCYENLFANCCEVCSLPIGCNCKDLSYKDRHWHENCFKCAKCSRSLVDKPFAAKDELMLCTECYSHEYSSKCSTCKKTVMPG--SRKMEYKGNSWHETCFLCQRCQQPIGTKSFIPKDNNYFCVPCFEKQFAYQCCACKKAI--TTGGVTYHDKPWHRECFTCIGCKRQLAGQRFTSRENYPYCLDCFSNLYAKKCVGCTKAITSLAGAKYISFEERQWHSECFTCMQCSVSLVGRGFLTQRDDILCTDCGREK 280
BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Match: fhl2a (four and a half LIM domains 2a [Source:ZFIN;Acc:ZDB-GENE-040808-49]) HSP 1 Score: 336.65 bits (862), Expect = 4.911e-111 Identity = 148/273 (54.21%), Postives = 201/273 (73.63%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
+ C C SL G +Y+L++D P C+ CYES Y+N C++C IGC+S+DLS+KDRHWHE CF CF+C SLVD+PF+TKD L C++CY N+++++C C K G RK E++G WHE CF C C+QPIGTKSFIP++ CVPCYE+++A +C C I GGVTY PWHK+CF CT C +QL+G +FTS+++ YC C+ L+AKKC+ CT PI+G GG K+ISFE+R WH++CF C KC++SLVG+GFLT+ D I+CP+CG+
Sbjct: 5 YDCHYCKESLFGKKYVLREDNPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEDCFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPECGK 277 HSP 2 Score: 151.369 bits (381), Expect = 7.008e-41 Identity = 88/273 (32.23%), Postives = 126/273 (46.15%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAEC 1465
+ C C E L Y + PYCV+ Y L C C + I + + H H F C C SL + KD+Q LC CY ++Y++ C +C I S+ + K WHE CF C C + + F KDN+ YC CY+ +FA +C C K G Y + WH++CFLC C+Q + + F R+ C+ C+ YA+KC C I G ++++ WH +CF C CS L G F + + C EC
Sbjct: 5 YDCHYCKESLFGKKYVLREDNPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEDCFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTG--GVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275 HSP 3 Score: 110.153 bits (274), Expect = 2.661e-26 Identity = 64/219 (29.22%), Postives = 100/219 (45.66%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
WH CF C +C LVD + + C Y+ +C C + I +R ME + + H F C+ C + +I KD+ C+ CYE Q+A C C I + +++ D+ WH+ CF C C L Q F ++D+ YC +C+ N +A +C C G + + +QWH +CF C C + + F+ DI+C C
Sbjct: 61 WHEDCFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPG--SRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPI--TTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275 HSP 4 Score: 95.1301 bits (235), Expect = 3.463e-21 Identity = 60/181 (33.15%), Postives = 89/181 (49.17%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIG--CDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDC 1105
C +C+ +I G + ++EH N SWH CF C C + + + N YCV Y + +C C + I T T +Q H F C C L+G R+ +DD C+ C+ + YA C C+ I SK +SF++R WH CF C +CS SLV + F T +++ C +C
Sbjct: 101 CHECKKTIMPG--SRKMEHKGN---SWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTGGVT-YHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275 HSP 5 Score: 49.6766 bits (117), Expect = 5.327e-6 Identity = 34/124 (27.42%), Postives = 49/124 (39.52%), Query Frame = 2
Query: 560 ALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIF---TEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGC 922
A+ C C+ I G + WH CF CT C + L + + YC+ + L +CA+C I +Y E++ H F CK C SL G ++ D LC C
Sbjct: 159 AMQCVHCKKPITTGGVTYH-------DQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275
BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Match: fhl2a (four and a half LIM domains 2a [Source:ZFIN;Acc:ZDB-GENE-040808-49]) HSP 1 Score: 336.65 bits (862), Expect = 4.911e-111 Identity = 148/273 (54.21%), Postives = 201/273 (73.63%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
+ C C SL G +Y+L++D P C+ CYES Y+N C++C IGC+S+DLS+KDRHWHE CF CF+C SLVD+PF+TKD L C++CY N+++++C C K G RK E++G WHE CF C C+QPIGTKSFIP++ CVPCYE+++A +C C I GGVTY PWHK+CF CT C +QL+G +FTS+++ YC C+ L+AKKC+ CT PI+G GG K+ISFE+R WH++CF C KC++SLVG+GFLT+ D I+CP+CG+
Sbjct: 5 YDCHYCKESLFGKKYVLREDNPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEDCFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPECGK 277 HSP 2 Score: 151.369 bits (381), Expect = 7.008e-41 Identity = 88/273 (32.23%), Postives = 126/273 (46.15%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAEC 1465
+ C C E L Y + PYCV+ Y L C C + I + + H H F C C SL + KD+Q LC CY ++Y++ C +C I S+ + K WHE CF C C + + F KDN+ YC CY+ +FA +C C K G Y + WH++CFLC C+Q + + F R+ C+ C+ YA+KC C I G ++++ WH +CF C CS L G F + + C EC
Sbjct: 5 YDCHYCKESLFGKKYVLREDNPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEDCFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTG--GVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275 HSP 3 Score: 110.153 bits (274), Expect = 2.661e-26 Identity = 64/219 (29.22%), Postives = 100/219 (45.66%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
WH CF C +C LVD + + C Y+ +C C + I +R ME + + H F C+ C + +I KD+ C+ CYE Q+A C C I + +++ D+ WH+ CF C C L Q F ++D+ YC +C+ N +A +C C G + + +QWH +CF C C + + F+ DI+C C
Sbjct: 61 WHEDCFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPG--SRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPI--TTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275 HSP 4 Score: 95.1301 bits (235), Expect = 3.463e-21 Identity = 60/181 (33.15%), Postives = 89/181 (49.17%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIG--CDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDC 1105
C +C+ +I G + ++EH N SWH CF C C + + + N YCV Y + +C C + I T T +Q H F C C L+G R+ +DD C+ C+ + YA C C+ I SK +SF++R WH CF C +CS SLV + F T +++ C +C
Sbjct: 101 CHECKKTIMPG--SRKMEHKGN---SWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTGGVT-YHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275 HSP 5 Score: 49.6766 bits (117), Expect = 5.327e-6 Identity = 34/124 (27.42%), Postives = 49/124 (39.52%), Query Frame = 2
Query: 560 ALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIF---TEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGC 922
A+ C C+ I G + WH CF CT C + L + + YC+ + L +CA+C I +Y E++ H F CK C SL G ++ D LC C
Sbjct: 159 AMQCVHCKKPITTGGVTYH-------DQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275
BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Match: fhl2a (four and a half LIM domains 2a [Source:ZFIN;Acc:ZDB-GENE-040808-49]) HSP 1 Score: 336.65 bits (862), Expect = 4.911e-111 Identity = 148/273 (54.21%), Postives = 201/273 (73.63%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
+ C C SL G +Y+L++D P C+ CYES Y+N C++C IGC+S+DLS+KDRHWHE CF CF+C SLVD+PF+TKD L C++CY N+++++C C K G RK E++G WHE CF C C+QPIGTKSFIP++ CVPCYE+++A +C C I GGVTY PWHK+CF CT C +QL+G +FTS+++ YC C+ L+AKKC+ CT PI+G GG K+ISFE+R WH++CF C KC++SLVG+GFLT+ D I+CP+CG+
Sbjct: 5 YDCHYCKESLFGKKYVLREDNPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEDCFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPECGK 277 HSP 2 Score: 151.369 bits (381), Expect = 7.008e-41 Identity = 88/273 (32.23%), Postives = 126/273 (46.15%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAEC 1465
+ C C E L Y + PYCV+ Y L C C + I + + H H F C C SL + KD+Q LC CY ++Y++ C +C I S+ + K WHE CF C C + + F KDN+ YC CY+ +FA +C C K G Y + WH++CFLC C+Q + + F R+ C+ C+ YA+KC C I G ++++ WH +CF C CS L G F + + C EC
Sbjct: 5 YDCHYCKESLFGKKYVLREDNPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEDCFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTG--GVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275 HSP 3 Score: 110.153 bits (274), Expect = 2.661e-26 Identity = 64/219 (29.22%), Postives = 100/219 (45.66%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
WH CF C +C LVD + + C Y+ +C C + I +R ME + + H F C+ C + +I KD+ C+ CYE Q+A C C I + +++ D+ WH+ CF C C L Q F ++D+ YC +C+ N +A +C C G + + +QWH +CF C C + + F+ DI+C C
Sbjct: 61 WHEDCFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPG--SRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPI--TTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275 HSP 4 Score: 95.1301 bits (235), Expect = 3.463e-21 Identity = 60/181 (33.15%), Postives = 89/181 (49.17%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIG--CDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDC 1105
C +C+ +I G + ++EH N SWH CF C C + + + N YCV Y + +C C + I T T +Q H F C C L+G R+ +DD C+ C+ + YA C C+ I SK +SF++R WH CF C +CS SLV + F T +++ C +C
Sbjct: 101 CHECKKTIMPG--SRKMEHKGN---SWHETCFTCQRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTGGVT-YHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275 HSP 5 Score: 49.6766 bits (117), Expect = 5.327e-6 Identity = 34/124 (27.42%), Postives = 49/124 (39.52%), Query Frame = 2
Query: 560 ALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIF---TEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGC 922
A+ C C+ I G + WH CF CT C + L + + YC+ + L +CA+C I +Y E++ H F CK C SL G ++ D LC C
Sbjct: 159 AMQCVHCKKPITTGGVTYH-------DQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTDRDDILCPEC 275
BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Match: fhl2b (four and a half LIM domains 2b [Source:ZFIN;Acc:ZDB-GENE-041010-121]) HSP 1 Score: 336.265 bits (861), Expect = 5.710e-111 Identity = 148/273 (54.21%), Postives = 202/273 (73.99%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
+ C C SL G +Y+L+D+ P C+ CYES Y+N C++C IGC+S+DLS+KDRHWH+ CF CF+C SLVD+PF+TKD L C++CY N+++++C C K G RK E++G WHE CF C C+QPIGTKSFIP++ CVPCYE+++A +C +C I GGVTY PWHK+CF CT C +QL+G +FTS+++ PYC C+ L+AKKC+ CT PI+G GG K+ISFE+R WH++CF C KC++SLVG+GFLT D I+CP+CG+
Sbjct: 5 YDCHYCKESLFGKKYVLRDENPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPECGK 277 HSP 2 Score: 149.058 bits (375), Expect = 5.737e-40 Identity = 88/273 (32.23%), Postives = 126/273 (46.15%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAEC 1465
+ C C E L Y PYCV+ Y L C C + I + + H H F C C SL + KD+Q LC CY ++Y++ C +C I S+ + K WHE CF C C + + F KDN+ YC CY+ +FA +C C K G Y + WH++CFLC C+Q + + F R+ C+ C+ YA+KC C I G ++++ WH +CF C CS L G F ++ + C EC
Sbjct: 5 YDCHYCKESLFGKKYVLRDENPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTG--GVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275 HSP 3 Score: 110.923 bits (276), Expect = 1.486e-26 Identity = 65/219 (29.68%), Postives = 101/219 (46.12%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
WH CF C +C LVD + + C Y+ +C C + I +R ME + + H F C+ C + +I KD+ C+ CYE Q+A C QC I + +++ D+ WH+ CF C C L Q F ++D+ YC +C+ N +A +C C G + + +QWH +CF C C + + F+ DI+C C
Sbjct: 61 WHDDCFHCFKCHRSLVDKPFSTKDEQLLCTECYSNEYSSKCFECKKTIMPG--SRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPI--TTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275 HSP 4 Score: 98.5969 bits (244), Expect = 1.934e-22 Identity = 61/181 (33.70%), Postives = 91/181 (50.28%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIG--CDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDC 1105
C +C+ +I G + ++EH N SWH CF C C + + + N YCV Y + +C C + I T T +Q H F C C L+G R+ +DD P C+ C+ + YA C C+ I SK +SF++R WH CF C +CS SLV + F T+ +++ C +C
Sbjct: 101 CFECKKTIMPG--SRKMEHKGN---SWHETCFTCQRCQQPIGTKSFIPKDNSNYCVPCYEKQFALQCVQCKKPITTGGVT-YHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275 HSP 5 Score: 55.0694 bits (131), Expect = 8.789e-8 Identity = 37/124 (29.84%), Postives = 51/124 (41.13%), Query Frame = 2
Query: 560 ALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIF---TEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGC 922
AL C QC+ I G + WH CF CT C + L + + PYC+ + L +CA C I +Y E++ H F CK C SL G ++ + D LC C
Sbjct: 159 ALQCVQCKKPITTGGVTYH-------DQPWHKDCFLCTGCKQQLSGQRFTSRDDFPYCLNCFCNLYAKKCAACTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275
BLAST of Four and A half lim domains vs. Ensembl Xenopus
Match: fhl2 (four and a half LIM domains 2 [Source:NCBI gene;Acc:100145283]) HSP 1 Score: 340.887 bits (873), Expect = 1.272e-112 Identity = 153/273 (56.04%), Postives = 198/273 (72.53%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
F C C SL G +Y+L+++ P C+ CYES Y+N C++C IGCD KDLS+KDRHWHE CF CF+C SLVD+PFA KD +L C++CY N+++++C C K G RK EY+G WHE CF+C C+QPIGTKSFIP++ CV CYE+++A C +C I GGVTY+ PWHKECF CT C K L+G +FTS++E YC C+ L+AKKC+ CT PI+G GG K+ISFE+R WHS+CF C KC ISLVG+GFLT D I+CP+CG+
Sbjct: 5 FDCHYCKESLFGKKYLLREENPYCVKCYESLYSNSCEECKKPIGCDGKDLSYKDRHWHESCFHCFQCKKSLVDKPFAAKDEHLICTECYSNEYSSKCSECKKTIMPGTRKMEYKGNSWHETCFICSRCQQPIGTKSFIPKDNQNFCVACYEKQFAMHCVQCKKAITTGGVTYRDQPWHKECFICTGCKKPLSGQRFTSRDEFAYCLNCFCNLYAKKCAGCTNPISGLGGAKYISFEERQWHSDCFNCKKCAISLVGRGFLTERDDILCPECGK 277 HSP 2 Score: 154.451 bits (389), Expect = 9.258e-42 Identity = 85/273 (31.14%), Postives = 129/273 (47.25%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAEC 1465
F C C E L Y PYCV+ Y L C C + I + + + H H + F C C SL + KD+ +C CY ++Y++ C +C I ++ + +K WHE CF C C + + F KDN +C CY+ +FA C C K G YR + WH+ECF+C C++P+ + F R++ C+ C+ YA+KC C I G ++++ WH +CF C C+ L G F ++ + C EC
Sbjct: 5 FDCHYCKESLFGKKYLLREENPYCVKCYESLYSNSCEECKKPIGCDGKDLSYKDRHWHESCFHCFQCKKSLVDKPFAAKDEHLICTECYSNEYSSKCSECKKTIMPGTRKMEYKGNSWHETCFICSRCQQPIGTKSFIPKDNQNFCVACYEKQFAMHCVQCKKAITTG--GVTYRDQPWHKECFICTGCKKPLSGQRFTSRDEFAYCLNCFCNLYAKKCAGCTNPISGLGGAKYISFEERQWHSDCFNCKKCAISLVGRGFLTERDDILCPEC 275 HSP 3 Score: 120.939 bits (302), Expect = 6.790e-30 Identity = 68/219 (31.05%), Postives = 104/219 (47.49%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
WH CF C +C + LVD + C Y+ +C+ C + I TR ME + + H F C C + +I KD+Q C+ CYE Q+A C QC I + ++++D+ WH++CF C C L Q F ++D YC +C+ N +A +C GC G + + +QWH +CF C C + + F+ DI+C C
Sbjct: 61 WHESCFHCFQCKKSLVDKPFAAKDEHLICTECYSNEYSSKCSECKKTIMPG--TRKMEYKGNSWHETCFICSRCQQPIGTKSFIPKDNQNFCVACYEKQFAMHCVQCKKAI--TTGGVTYRDQPWHKECFICTGCKKPLSGQRFTSRDEFAYCLNCFCNLYAKKCAGCTNPISGLGGAKYISFEERQWHSDCFNCKKCAISLVGRGFLTERDDILCPEC 275 HSP 4 Score: 95.1301 bits (235), Expect = 4.663e-21 Identity = 53/167 (31.74%), Postives = 83/167 (49.70%), Query Frame = 2
Query: 1208 FLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG--VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR*MVIFHYIRLQQLI 1702
F C C++ + K ++ RE++ CV CYE Y+ C +C I G ++YK WH+ CF C C K L F +K+E C ECY ++ KCS+C K I G + + ++ WH CF C +C + K F+ D++ C C H ++ ++ I
Sbjct: 5 FDCHYCKESLFGKKYLLREENPYCVKCYESLYSNSCEECKKPIGCDGKDLSYKDRHWHESCFHCFQCKKSLVDKPFAAKDEHLICTECYSNEYSSKCSECKKTI--MPGTRKMEYKGNSWHETCFICSRCQQPIGTKSFIPKDNQNFCVACYEKQFAMHCVQCKKAI 169 HSP 5 Score: 91.6633 bits (226), Expect = 6.935e-20 Identity = 55/181 (30.39%), Postives = 91/181 (50.28%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIG--CDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDC 1105
C++C+ +I G ++E+ N SWH CF C+ C + + + N+ +CV Y + C C + I T T +Q H F C C L+G R+ +D+ C+ C+ + YA C C+ I +K +SF++R WH CF C +C+ SLV + F T+ +++ C +C
Sbjct: 101 CSECKKTIMPG--TRKMEYKGN---SWHETCFICSRCQQPIGTKSFIPKDNQNFCVACYEKQFAMHCVQCKKAITTGGVT-YRDQPWHKECFICTGCKKPLSGQRFTSRDEFAYCLNCFCNLYAKKCAGCTNPISGLGGAKYISFEERQWHSDCFNCKKCAISLVGRGFLTERDDILCPEC 275 HSP 6 Score: 53.5286 bits (127), Expect = 4.072e-7 Identity = 39/141 (27.66%), Postives = 58/141 (41.13%), Query Frame = 2
Query: 509 RNNQDLGVGHVYRLDMAALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIF---TEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGC 922
++NQ+ V + A+ C QC+ +I G + R WH CF CT C + L + YC+ + L +CA C I +Y E++ H F CK C SL G ++ + D LC C
Sbjct: 144 KDNQNFCVACYEK--QFAMHCVQCKKAITTGGVTYR-------DQPWHKECFICTGCKKPLSGQRFTSRDEFAYCLNCFCNLYAKKCAGCTNPISGLGGAKYISFEERQWHSDCFNCKKCAISLVGRGFLTERDDILCPEC 275
BLAST of Four and A half lim domains vs. Ensembl Xenopus
Match: shroom3 (shroom family member 3 [Source:Xenbase;Acc:XB-GENE-490224]) HSP 1 Score: 325.865 bits (834), Expect = 8.510e-107 Identity = 147/272 (54.04%), Postives = 194/272 (71.32%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCG 1657
F C SC SL G +YI ++ P CI CY+S +AN C +C IG D ++L ++DRH+HE CF+CF C SL D+PF +D L C+DCY N+F+++C C K G RK EY G+ WHE CF+C C+QPIG++SFIP Q+ C+PCYE K A +CT C + +GGVTY+ PWHKECF CT C QLAG +FTS++EKPYC +C+G L+AKKC+ CTKPITGFGG K++SFE+RHWH CF C +C+ SLVGKGF+ +++ I+C C
Sbjct: 5 FDCDSCKESLYGRKYIQMEEGPYCIPCYDSLFANTCDECKELIGHDCRELYYEDRHYHEHCFRCFRCDHSLADEPFTCQDEELLCNDCYCNEFSSKCISCEKTVMPGSRKLEYNGQTWHEHCFICNSCQQPIGSRSFIPENQNHYCIPCYESKLAPRCTHCKKSLTKGGVTYRDEPWHKECFVCTGCKTQLAGQQFTSQDEKPYCIKCFGNLYAKKCAGCTKPITGFGGAKYVSFEERHWHHSCFNCSRCSTSLVGKGFIPDNEDILCRACN 276 HSP 2 Score: 150.984 bits (380), Expect = 1.374e-40 Identity = 88/277 (31.77%), Postives = 128/277 (46.21%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH-FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGEL 1477
F C C E L Y PYC+ Y L C C ELI + E H+ H F C CD+SL + +D++ LC CY +++++ C C + S+ L + + WHE CF C C + + F ++ N YC CY++K A RC C K G YR + WH+ECF+C C+ + + F +++ C+ C+ YA+KC C I G V+++ WH CF C+ CS L G F E C C +L
Sbjct: 5 FDCDSCKESLYGRKYIQMEEGPYCIPCYDSLFANTCDECKELIGHDCRELYYEDRHYHEHCFRCFRCDHSLADEPFTCQDEELLCNDCYCNEFSSKCISCEKTVMPGSRKLEYNGQTWHEHCFICNSCQQPIGSRSFIPENQNHYCIPCYESKLAPRCTHCKKSLTKG--GVTYRDEPWHKECFVCTGCKTQLAGQQFTSQDEKPYCIKCFGNLYAKKCAGCTKPITGFGGAKYVSFEERHWHHSCFNCSRCSTSLVGKGFIPDNEDILCRACNSDL 279 HSP 3 Score: 112.849 bits (281), Expect = 3.540e-27 Identity = 65/219 (29.68%), Postives = 100/219 (45.66%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAME---QEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
+H CF+C CD L D + + C Y +C +C++ + +R +E Q H F C SC + +I ++ CI CYES+ A C C + ++++D WH++CF C C + L Q F ++D YC C+ N +A +C GC K G + + + WH CF C C + K FIP +DI+C C
Sbjct: 61 YHEHCFRCFRCDHSLADEPFTCQDEELLCNDCYCNEFSSKCISCEKTVMPG--SRKLEYNGQTWHEHCFICNSCQQPIGSRSFIPENQNHYCIPCYESKLAPRCTHCKKSL--TKGGVTYRDEPWHKECFVCTGCKTQLAGQQFTSQDEKPYCIKCFGNLYAKKCAGCTKPITGFGGAKYVSFEERHWHHSCFNCSRCSTSLVGKGFIPDNEDILCRAC 275 HSP 4 Score: 91.6633 bits (226), Expect = 8.375e-20 Identity = 53/181 (29.28%), Postives = 88/181 (48.62%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIG--CDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDC 1105
C C+ ++ G + ++E+ T WH CF C C + + + + YC+ Y + PRC C + + T+ ++ H F C C L G ++ +D++P CI C+ + YA C C+ I +K +SF++RHWH CF C CS+SLV + F + ++ C C
Sbjct: 101 CISCEKTVMPG--SRKLEYNGQT---WHEHCFICNSCQQPIGSRSFIPENQNHYCIPCYESKLAPRCTHCKKSL-TKGGVTYRDEPWHKECFVCTGCKTQLAGQQFTSQDEKPYCIKCFGNLYAKKCAGCTKPITGFGGAKYVSFEERHWHHSCFNCSRCSTSLVGKGFIPDNEDILCRAC 275
BLAST of Four and A half lim domains vs. Ensembl Xenopus
Match: fhl5 (four and a half LIM domains 5 [Source:NCBI gene;Acc:100144736]) HSP 1 Score: 322.783 bits (826), Expect = 1.543e-105 Identity = 145/272 (53.31%), Postives = 191/272 (70.22%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCG 1657
F C C SL G +Y LKDD P CI C+ S +AN+C++C I C+SKDL++KD HWHE CFKC +C SLV++PFA KD L C +CY +++++C GC G RK EY G WHE CF+C CR+P+G K FIP+E I C+PCYE+++A +C C I +GG++++ WH+ECF CT C K L G K TS++E PYC +C+ L+AKKC+ C KPITG GG K+ISFEDR WHS+CFTC KC+ SLVG+ F TN+D ++CP CG
Sbjct: 6 FDCFHCKESLYGKKYTLKDDIPYCIKCFNSLFANLCERCKKPIECNSKDLAYKDSHWHETCFKCDKCDHSLVEKPFAAKDELLLCIECYSTEYSSKCFGCRATIMPGSRKMEYNGSNWHETCFVCQSCREPVGNKPFIPKESKIYCMPCYEKQFANQCKSCRKAITKGGLSFQEQQWHRECFVCTSCKKNLVGEKSTSRDESPYCVDCFDNLYAKKCAACAKPITGQGGAKYISFEDRQWHSDCFTCAKCSKSLVGEKFHTNEDDVLCPSCG 277 HSP 2 Score: 165.622 bits (418), Expect = 8.495e-46 Identity = 89/273 (32.60%), Postives = 135/273 (49.45%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAEC 1465
F C C E L Y + PYC++ + L C C + I A + H H F C CD+SL + KD+ LCI CY ++Y++ C C I S+ + + +WHE CF C C + ++PF K++ +YC CY+ +FA +C C K G ++ +QWH ECF+C C++ + + R++ CV C++ YA+KC C I G ++++ WH +CFTC CSK L G KF + E+ C C
Sbjct: 6 FDCFHCKESLYGKKYTLKDDIPYCIKCFNSLFANLCERCKKPIECNSKDLAYKDSHWHETCFKCDKCDHSLVEKPFAAKDELLLCIECYSTEYSSKCFGCRATIMPGSRKMEYNGSNWHETCFVCQSCREPVGNKPFIPKESKIYCMPCYEKQFANQCKSCRKAITKG--GLSFQEQQWHRECFVCTSCKKNLVGEKSTSRDESPYCVDCFDNLYAKKCAACAKPITGQGGAKYISFEDRQWHSDCFTCAKCSKSLVGEKFHTNEDDVLCPSC 276 HSP 3 Score: 128.257 bits (321), Expect = 1.999e-32 Identity = 77/252 (30.56%), Postives = 120/252 (47.62%), Query Frame = 2
Query: 554 MAALPCTQCQSSIQ--AGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAME---QEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVF--KAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
+ A C +C+ I+ + LA + H WH CFKC +CD LV+ + C+ Y+ +C C I +R ME H F C+SC + +I K+ + C+ CYE Q+AN C+ C I LSF+++ WH +CF C C +LV + ++D + YC DC+DN +A +C C K + G + + +QWH +CF C C + + + F E D++C C
Sbjct: 36 LFANLCERCKKPIECNSKDLAYKDSH-------WHETCFKCDKCDHSLVEKPFAAKDELLLCIECYSTEYSSKCFGCRATIMPG--SRKMEYNGSNWHETCFVCQSCREPVGNKPFIPKESKIYCMPCYEKQFANQCKSCRKAI--TKGGLSFQEQQWHRECFVCTSCKKNLVGEKSTSRDESPYCVDCFDNLYAKKCAACAKPITGQGGAKYISFEDRQWHSDCFTCAKCSKSLVGEKFHTNEDDVLCPSC 276
BLAST of Four and A half lim domains vs. Ensembl Xenopus
Match: fhl1 (four and a half LIM domains 1 [Source:NCBI gene;Acc:448334]) HSP 1 Score: 266.929 bits (681), Expect = 8.232e-84 Identity = 122/276 (44.20%), Postives = 173/276 (62.68%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFG-GCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR*M 1666
F C C L G +YI KD C+ C++ AN C +C IG DSK+L +K+R+WH+ CF+C +C L ++ F KDN + C+ C + + RC GCHK + G R EY+G WHEECF C +C+Q IG+ SF P+ D+ CV C+E+K+A+ C KC I GG+TY+ PWH +CF C C K+LAG +FT+ E+ YC +CY AKKC+ C PITGFG G +++E WH CFTC KC+++L K F+ +++++ C DC + M
Sbjct: 21 FDCHYCRAPLQGKKYIEKDGHNTCVKCFDKICANTCAECRKPIGVDSKELHYKNRYWHDNCFRCAKCYHPLANEQFIAKDNKIMCAKCTTREDSLRCSGCHKQIQPGGRNVEYKGSAWHEECFTCSNCKQAIGSGSFFPKGTDVYCVTCHEQKFAKNCVKCNNPITSGGITYQDQPWHGDCFVCETCHKKLAGQRFTAVEDHYYCVDCYKSFVAKKCAGCNNPITGFGKGSNVVNYEGNSWHEYCFTCKKCSLNLANKRFVRHNEQVYCQDCAKKM 296 HSP 2 Score: 107.071 bits (266), Expect = 4.869e-25 Identity = 63/220 (28.64%), Postives = 99/220 (45.00%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKV---FKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
WH CF+C +C L + + NK C + + RC+ C + I + R +E + H F C +C ++ + K C+ C+E ++A C +C+ I S ++++D+ WH CF C C L Q F +++ YC DCY + A +C GC+ F G Y G WHE CF C C + K F+ + + C C
Sbjct: 77 WHDNCFRCAKCYHPLANEQFIAKDNKIMCAKCTTREDSLRCSGCHKQI--QPGGRNVEYKGSAWHEECFTCSNCKQAIGSGSFFPKGTDVYCVTCHEQKFAKNCVKCNNPI--TSGGITYQDQPWHGDCFVCETCHKKLAGQRFTAVEDHYYCVDCYKSFVAKKCAGCNNPITGFGKGSNVVNYEGNSWHEYCFTCKKCSLNLANKRFVRHNEQVYCQDC 292
BLAST of Four and A half lim domains vs. Ensembl Xenopus
Match: fhl1 (four and a half LIM domains 1 [Source:NCBI gene;Acc:448334]) HSP 1 Score: 266.544 bits (680), Expect = 1.049e-83 Identity = 122/276 (44.20%), Postives = 173/276 (62.68%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFG-GCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR*M 1666
F C C L G +YI KD C+ C++ AN C +C IG DSK+L +K+R+WH+ CF+C +C L ++ F KDN + C+ C + + RC GCHK + G R EY+G WHEECF C +C+Q IG+ SF P+ D+ CV C+E+K+A+ C KC I GG+TY+ PWH +CF C C K+LAG +FT+ E+ YC +CY AKKC+ C PITGFG G +++E WH CFTC KC+++L K F+ +++++ C DC + M
Sbjct: 16 FDCHYCRAPLQGKKYIEKDGHNTCVKCFDKICANTCAECRKPIGVDSKELHYKNRYWHDNCFRCAKCYHPLANEQFIAKDNKIMCAKCTTREDSLRCSGCHKQIQPGGRNVEYKGSAWHEECFTCSNCKQAIGSGSFFPKGTDVYCVTCHEQKFAKNCVKCNNPITSGGITYQDQPWHGDCFVCETCHKKLAGQRFTAVEDHYYCVDCYKSFVAKKCAGCNNPITGFGKGSNVVNYEGNSWHEYCFTCKKCSLNLANKRFVRHNEQVYCQDCAKKM 291 HSP 2 Score: 130.954 bits (328), Expect = 2.295e-33 Identity = 80/278 (28.78%), Postives = 127/278 (45.68%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG-----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGEL 1477
F C C L Y CV+ + ++ CA C + I + + + H F C C + L ++I KD++ +C C + + C C +I +++ +K WHE+CF C C ++ F K ++YC C++ KFA C C+ +G Y+ + WH +CF+C C + + + F E CV CY+ A+KC C I G V Y+G+ WH+ CFTC CS LA +F E+ YC +C ++
Sbjct: 16 FDCHYCRAPLQGKKYIEKDGHNTCVKCFDKICANTCAECRKPIGVDSKELHYKNRYWHDNCFRCAKCYHPLANEQFIAKDNKIMCAKCTTREDSLRCSGCHKQIQPGGRNVEYKGSAWHEECFTCSNCKQAIGSGSFFPKGTDVYCVTCHEQKFAKNCVKCNNPITSG--GITYQDQPWHGDCFVCETCHKKLAGQRFTAVEDHYYCVDCYKSFVAKKCAGCNNPITGFGKGSNVVNYEGNSWHEYCFTCKKCSLNLANKRFVRHNEQVYCQDCAKKM 291 HSP 3 Score: 107.071 bits (266), Expect = 4.674e-25 Identity = 63/220 (28.64%), Postives = 99/220 (45.00%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKV---FKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
WH CF+C +C L + + NK C + + RC+ C + I + R +E + H F C +C ++ + K C+ C+E ++A C +C+ I S ++++D+ WH CF C C L Q F +++ YC DCY + A +C GC+ F G Y G WHE CF C C + K F+ + + C C
Sbjct: 72 WHDNCFRCAKCYHPLANEQFIAKDNKIMCAKCTTREDSLRCSGCHKQI--QPGGRNVEYKGSAWHEECFTCSNCKQAIGSGSFFPKGTDVYCVTCHEQKFAKNCVKCNNPI--TSGGITYQDQPWHGDCFVCETCHKKLAGQRFTAVEDHYYCVDCYKSFVAKKCAGCNNPITGFGKGSNVVNYEGNSWHEYCFTCKKCSLNLANKRFVRHNEQVYCQDC 287
BLAST of Four and A half lim domains vs. Ensembl Mouse
Match: Fhl2 (four and a half LIM domains 2 [Source:MGI Symbol;Acc:MGI:1338762]) HSP 1 Score: 348.591 bits (893), Expect = 1.026e-115 Identity = 157/273 (57.51%), Postives = 201/273 (73.63%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
F C C+ SL G +YILK++ P C+ C+E YAN C++C IGCD KDLS+KDRHWHE CF C C SSLVD+PFA K+ L C+DCY N+++++C C K G RK EY+G WHE CF C C+QPIGTKSFIP+E CVPCYE++YA +C +C I GGVTY+ PWHKECF CT C KQL+G +FT+++E PYC C+ +L+AKKC+ CT PI+G GG K+ISFE+R WH++CF C KC++SLVG+GFLT D I+CPDCG+
Sbjct: 5 FDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGK 277 HSP 2 Score: 147.517 bits (371), Expect = 2.233e-39 Identity = 82/273 (30.04%), Postives = 132/273 (48.35%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAEC 1465
F C C+E L Y P+CV + +L C C I + + + H H F C C +SL + K++Q LC CY ++Y++ CQ+C I ++ + +K WHE CF C C + + F K+N +C CY+ ++A +C C K G YR + WH+ECF+C C++ + + F R++ C+ C+ + YA+KC C I G ++++ WH +CF C CS L G F ++ + C +C
Sbjct: 5 FDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTG--GVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 114.39 bits (285), Expect = 7.641e-28 Identity = 66/219 (30.14%), Postives = 101/219 (46.12%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
WH CF C+ C LVD + + C Y+ +C C + I TR ME + H F C+ C + +I K++Q C+ CYE QYA C QC I + ++++++ WH++CF C C L Q F +D YC C+ + +A +C GC G + + +QWH +CF C C + + F+ DI+C C
Sbjct: 61 WHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPI--TTGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 4 Score: 95.1301 bits (235), Expect = 3.703e-21 Identity = 57/181 (31.49%), Postives = 89/181 (49.17%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIG--CDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDC 1105
C +C+ +I G + + + SWH CF C C + + + N+ +CV Y + +C C + I T T EQ H F C +C L+G R+ +D+ P C+ C+ YA C C+ I +K +SF++R WH CF C +CS SLV + F T+ +++ C DC
Sbjct: 101 CQECKKTIMPGTRKMEYKGS-----SWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTGGVT-YREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 5 Score: 57.3806 bits (137), Expect = 1.747e-8 Identity = 38/124 (30.65%), Postives = 52/124 (41.94%), Query Frame = 2
Query: 560 ALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIF---TEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGC 922
AL C QC+ I G + R + WH CF CT C + L + PYC+ + L +CA C I +Y E++ H F CK C SL G ++ + D LC C
Sbjct: 159 ALQCVQCKKPITTGGVTYR-------EQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. Ensembl Mouse
Match: Fhl2 (four and a half LIM domains 2 [Source:MGI Symbol;Acc:MGI:1338762]) HSP 1 Score: 348.591 bits (893), Expect = 1.026e-115 Identity = 157/273 (57.51%), Postives = 201/273 (73.63%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
F C C+ SL G +YILK++ P C+ C+E YAN C++C IGCD KDLS+KDRHWHE CF C C SSLVD+PFA K+ L C+DCY N+++++C C K G RK EY+G WHE CF C C+QPIGTKSFIP+E CVPCYE++YA +C +C I GGVTY+ PWHKECF CT C KQL+G +FT+++E PYC C+ +L+AKKC+ CT PI+G GG K+ISFE+R WH++CF C KC++SLVG+GFLT D I+CPDCG+
Sbjct: 5 FDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGK 277 HSP 2 Score: 147.517 bits (371), Expect = 2.233e-39 Identity = 82/273 (30.04%), Postives = 132/273 (48.35%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAEC 1465
F C C+E L Y P+CV + +L C C I + + + H H F C C +SL + K++Q LC CY ++Y++ CQ+C I ++ + +K WHE CF C C + + F K+N +C CY+ ++A +C C K G YR + WH+ECF+C C++ + + F R++ C+ C+ + YA+KC C I G ++++ WH +CF C CS L G F ++ + C +C
Sbjct: 5 FDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTG--GVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 114.39 bits (285), Expect = 7.641e-28 Identity = 66/219 (30.14%), Postives = 101/219 (46.12%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
WH CF C+ C LVD + + C Y+ +C C + I TR ME + H F C+ C + +I K++Q C+ CYE QYA C QC I + ++++++ WH++CF C C L Q F +D YC C+ + +A +C GC G + + +QWH +CF C C + + F+ DI+C C
Sbjct: 61 WHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPI--TTGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 4 Score: 95.1301 bits (235), Expect = 3.703e-21 Identity = 57/181 (31.49%), Postives = 89/181 (49.17%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIG--CDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDC 1105
C +C+ +I G + + + SWH CF C C + + + N+ +CV Y + +C C + I T T EQ H F C +C L+G R+ +D+ P C+ C+ YA C C+ I +K +SF++R WH CF C +CS SLV + F T+ +++ C DC
Sbjct: 101 CQECKKTIMPGTRKMEYKGS-----SWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTGGVT-YREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 5 Score: 57.3806 bits (137), Expect = 1.747e-8 Identity = 38/124 (30.65%), Postives = 52/124 (41.94%), Query Frame = 2
Query: 560 ALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIF---TEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGC 922
AL C QC+ I G + R + WH CF CT C + L + PYC+ + L +CA C I +Y E++ H F CK C SL G ++ + D LC C
Sbjct: 159 ALQCVQCKKPITTGGVTYR-------EQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. Ensembl Mouse
Match: Fhl3 (four and a half LIM domains 3 [Source:MGI Symbol;Acc:MGI:1341092]) HSP 1 Score: 307.76 bits (787), Expect = 6.761e-100 Identity = 134/273 (49.08%), Postives = 187/273 (68.50%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
F C C+ SL G +YI D P C+ CY++ +AN C +C IG DS++L ++DRH+HE CF+C C SL D+PF +D+ L C++CY F+++C C + G RK EY G+ WHE CFLC C QP+G++SF+P + CVPCYE K+A +C +C + +GGVTY+ PWH+EC CT C LAG +FTS+++ PYC C+GELFA KCS C +PITG GG K++SFEDRHWH CF+C +C+ SLVG+GF+ + D+++C C +
Sbjct: 5 FDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNECYCTAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCKTPLAGQQFTSRDDDPYCVACFGELFAPKCSSCKRPITGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGCSQ 277 HSP 2 Score: 154.836 bits (390), Expect = 5.226e-42 Identity = 90/273 (32.97%), Postives = 123/273 (45.05%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAEC 1465
F C +C+E L Y + PYCV Y CA C +LI + E H H F C C SL + +D + LC CY + +++ C C + S+ L + + WHE CF C C L + F YC CY+NKFA RC C K G YR + WH EC +C C+ P+ + F R+ D CV C+ E +A KC+ C I G V+++ WH CF+C CS L G F ++ C C
Sbjct: 5 FDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNECYCTAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQG--GVTYRDQPWHRECLVCTGCKTPLAGQQFTSRDDDPYCVACFGELFAPKCSSCKRPITGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGC 275 HSP 3 Score: 97.4413 bits (241), Expect = 5.639e-22 Identity = 58/183 (31.69%), Postives = 85/183 (46.45%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQC----SGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDC 1105
C+ C ++ G + ++E+ T WH CF C+ C++ L + YCV Y PRCA C + + T+ +Q H C C L G ++ +DD P C+ C+ +A C C +G G K +SF+DRHWH CF C CS+SLV Q F + + C C
Sbjct: 101 CSACGETVMPG--SRKLEYGGQT---WHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTL-TQGGVTYRDQPWHRECLVCTGCKTPLAGQQFTSRDDDPYCVACFGELFAPKCSSCKRPITGLGG--GKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGC 275 HSP 4 Score: 59.6918 bits (143), Expect = 2.816e-9 Identity = 34/124 (27.42%), Postives = 54/124 (43.55%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIF---TEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYES 931
C +C ++ G + R WH C CT C L + + PYCV + +L P+C++C I +Y ++ H + F+C C SL G ++ DQ LC GC ++
Sbjct: 162 CARCSKTLTQGGVTYR-------DQPWHRECLVCTGCKTPLAGQQFTSRDDDPYCVACFGELFAPKCSSCKRPITGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGCSQA 278
BLAST of Four and A half lim domains vs. Ensembl Mouse
Match: Fhl5 (four and a half LIM domains 5 [Source:MGI Symbol;Acc:MGI:1913192]) HSP 1 Score: 304.294 bits (778), Expect = 1.790e-98 Identity = 141/274 (51.46%), Postives = 190/274 (69.34%), Query Frame = 2
Query: 836 AHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCG 1657
+ F C+ C +SL G +Y+LKDD CI CY+ ++N C+QC I DSKDL +K+RHWHE CF+C +C SLV++PF KD+ L C+DCY N+ +++C C + G RK E++G WHE CF+C CRQPIGTK I +E CVPC+E+++A C C VI GG+T++ WHKECF C+ C K+L F SK++ P+C +CY L+AKKC+ CTKPITG G KFI F+DR WHSECF C KC++SLVG+GFLT++ +I+C CG
Sbjct: 4 SQFDCQYCTSSLIGKKYVLKDDNLYCISCYDRIFSNYCEQCKEPIESDSKDLCYKNRHWHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVITSGGITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKCG 277 HSP 2 Score: 150.214 bits (378), Expect = 2.356e-40 Identity = 86/273 (31.50%), Postives = 133/273 (48.72%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIR--RGG--VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAEC 1465
F C C L+ Y + YC+ Y ++ C C E I ++ + H H F C C +SL ++ KDD+ LC CY ++ ++ C C I S+ + FK +WHE CF C C + +P +K++ YC C++ +FA C+ C KV +G +R + WH+ECFLC CR+ + ++F+ ++ C+ CY YA+KC C I RG + ++ WH ECF C CS L G F + + C +C
Sbjct: 6 FDCQYCTSSLIGKKYVLKDDNLYCISCYDRIFSNYCEQCKEPIESDSKDLCYKNRHWHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVITSG--GITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKC 276 HSP 3 Score: 107.457 bits (267), Expect = 2.497e-25 Identity = 69/247 (27.94%), Postives = 116/247 (46.96%), Query Frame = 2
Query: 569 CTQCQSSIQAGQ--LAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKA--GMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
C QC+ I++ L + H WH CF+C +C LV+ + ++ C Y+ +C C I +R ME + + H F C+ C + I K+ C+ C+E ++A+ C C I S ++F+D+ WH++CF C C L ++ F +KD+ +C DCY++ +A +C C K G + ++ +QWH ECF C C + + F+ +I+C C
Sbjct: 41 CEQCKEPIESDSKDLCYKNRH-------WHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKCFHCKRTIMPG--SRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVI--TSGGITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKC 276
BLAST of Four and A half lim domains vs. Ensembl Mouse
Match: Fhl5 (four and a half LIM domains 5 [Source:MGI Symbol;Acc:MGI:1913192]) HSP 1 Score: 304.294 bits (778), Expect = 1.790e-98 Identity = 141/274 (51.46%), Postives = 190/274 (69.34%), Query Frame = 2
Query: 836 AHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCG 1657
+ F C+ C +SL G +Y+LKDD CI CY+ ++N C+QC I DSKDL +K+RHWHE CF+C +C SLV++PF KD+ L C+DCY N+ +++C C + G RK E++G WHE CF+C CRQPIGTK I +E CVPC+E+++A C C VI GG+T++ WHKECF C+ C K+L F SK++ P+C +CY L+AKKC+ CTKPITG G KFI F+DR WHSECF C KC++SLVG+GFLT++ +I+C CG
Sbjct: 4 SQFDCQYCTSSLIGKKYVLKDDNLYCISCYDRIFSNYCEQCKEPIESDSKDLCYKNRHWHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVITSGGITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKCG 277 HSP 2 Score: 150.214 bits (378), Expect = 2.356e-40 Identity = 86/273 (31.50%), Postives = 133/273 (48.72%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIR--RGG--VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAEC 1465
F C C L+ Y + YC+ Y ++ C C E I ++ + H H F C C +SL ++ KDD+ LC CY ++ ++ C C I S+ + FK +WHE CF C C + +P +K++ YC C++ +FA C+ C KV +G +R + WH+ECFLC CR+ + ++F+ ++ C+ CY YA+KC C I RG + ++ WH ECF C CS L G F + + C +C
Sbjct: 6 FDCQYCTSSLIGKKYVLKDDNLYCISCYDRIFSNYCEQCKEPIESDSKDLCYKNRHWHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKCFHCKRTIMPGSRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVITSG--GITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKC 276 HSP 3 Score: 107.457 bits (267), Expect = 2.497e-25 Identity = 69/247 (27.94%), Postives = 116/247 (46.96%), Query Frame = 2
Query: 569 CTQCQSSIQAGQ--LAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKA--GMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
C QC+ I++ L + H WH CF+C +C LV+ + ++ C Y+ +C C I +R ME + + H F C+ C + I K+ C+ C+E ++A+ C C I S ++F+D+ WH++CF C C L ++ F +KD+ +C DCY++ +A +C C K G + ++ +QWH ECF C C + + F+ +I+C C
Sbjct: 41 CEQCKEPIESDSKDLCYKNRH-------WHEGCFRCNKCHHSLVEKPFVAKDDRLLCTDCYSNECSSKCFHCKRTIMPG--SRKMEFKGNYWHETCFVCEHCRQPIGTKPLISKESGNYCVPCFEKEFAHYCNFCKKVI--TSGGITFRDQIWHKECFLCSGCRKELYEEAFMSKDDFPFCLDCYNHLYAKKCAACTKPITGLRGAKFICFQDRQWHSECFNCGKCSVSLVGEGFLTHNMEILCRKC 276
BLAST of Four and A half lim domains vs. UniProt/SwissProt
Match: sp|O70433|FHL2_MOUSE (Four and a half LIM domains protein 2 OS=Mus musculus OX=10090 GN=Fhl2 PE=1 SV=1) HSP 1 Score: 348.591 bits (893), Expect = 7.176e-115 Identity = 157/273 (57.51%), Postives = 201/273 (73.63%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
F C C+ SL G +YILK++ P C+ C+E YAN C++C IGCD KDLS+KDRHWHE CF C C SSLVD+PFA K+ L C+DCY N+++++C C K G RK EY+G WHE CF C C+QPIGTKSFIP+E CVPCYE++YA +C +C I GGVTY+ PWHKECF CT C KQL+G +FT+++E PYC C+ +L+AKKC+ CT PI+G GG K+ISFE+R WH++CF C KC++SLVG+GFLT D I+CPDCG+
Sbjct: 5 FDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGK 277 HSP 2 Score: 147.517 bits (371), Expect = 1.562e-38 Identity = 82/273 (30.04%), Postives = 132/273 (48.35%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAEC 1465
F C C+E L Y P+CV + +L C C I + + + H H F C C +SL + K++Q LC CY ++Y++ CQ+C I ++ + +K WHE CF C C + + F K+N +C CY+ ++A +C C K G YR + WH+ECF+C C++ + + F R++ C+ C+ + YA+KC C I G ++++ WH +CF C CS L G F ++ + C +C
Sbjct: 5 FDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTG--GVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 114.39 bits (285), Expect = 5.345e-27 Identity = 66/219 (30.14%), Postives = 101/219 (46.12%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
WH CF C+ C LVD + + C Y+ +C C + I TR ME + H F C+ C + +I K++Q C+ CYE QYA C QC I + ++++++ WH++CF C C L Q F +D YC C+ + +A +C GC G + + +QWH +CF C C + + F+ DI+C C
Sbjct: 61 WHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPI--TTGGVTYREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 4 Score: 95.1301 bits (235), Expect = 2.590e-20 Identity = 57/181 (31.49%), Postives = 89/181 (49.17%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIG--CDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDC 1105
C +C+ +I G + + + SWH CF C C + + + N+ +CV Y + +C C + I T T EQ H F C +C L+G R+ +D+ P C+ C+ YA C C+ I +K +SF++R WH CF C +CS SLV + F T+ +++ C DC
Sbjct: 101 CQECKKTIMPGTRKMEYKGS-----SWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTGGVT-YREQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 5 Score: 57.3806 bits (137), Expect = 1.222e-7 Identity = 38/124 (30.65%), Postives = 52/124 (41.94%), Query Frame = 2
Query: 560 ALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIF---TEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGC 922
AL C QC+ I G + R + WH CF CT C + L + PYC+ + L +CA C I +Y E++ H F CK C SL G ++ + D LC C
Sbjct: 159 ALQCVQCKKPITTGGVTYR-------EQPWHKECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. UniProt/SwissProt
Match: sp|O35115|FHL2_RAT (Four and a half LIM domains protein 2 OS=Rattus norvegicus OX=10116 GN=Fhl2 PE=1 SV=1) HSP 1 Score: 347.821 bits (891), Expect = 1.682e-114 Identity = 156/273 (57.14%), Postives = 201/273 (73.63%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
F C C+ SL G +YILK++ P C+ C+E YAN C++C IGCD KDLS+KDRHWHE CF C C SSLVD+PFA K+ L C+DCY N+++++C C K G RK EY+G WHE CF C C+QPIGTKSFIP+E CVPCYE++YA +C +C I GGVTY+ PWH+ECF CT C KQL+G +FT+++E PYC C+ +L+AKKC+ CT PI+G GG K+ISFE+R WH++CF C KC++SLVG+GFLT D I+CPDCG+
Sbjct: 5 FDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTGGVTYRDQPWHRECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGK 277 HSP 2 Score: 147.132 bits (370), Expect = 1.777e-38 Identity = 82/273 (30.04%), Postives = 131/273 (47.99%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAEC 1465
F C C+E L Y P+CV + +L C C I + + + H H F C C +SL + K++Q LC CY ++Y++ CQ+C I ++ + +K WHE CF C C + + F K+N +C CY+ ++A +C C K G YR + WH ECF+C C++ + + F R++ C+ C+ + YA+KC C I G ++++ WH +CF C CS L G F ++ + C +C
Sbjct: 5 FDCHHCNESLYGKKYILKEENPHCVACFEELYANTCEECGTPIGCDCKDLSYKDRHWHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTG--GVTYRDQPWHRECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 115.546 bits (288), Expect = 2.342e-27 Identity = 67/219 (30.59%), Postives = 100/219 (45.66%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
WH CF C+ C LVD + + C Y+ +C C + I TR ME + H F C+ C + +I K++Q C+ CYE QYA C QC I + ++++D+ WH +CF C C L Q F +D YC C+ + +A +C GC G + + +QWH +CF C C + + F+ DI+C C
Sbjct: 61 WHEGCFHCSRCGSSLVDKPFAAKEEQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPI--TTGGVTYRDQPWHRECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 4 Score: 93.2041 bits (230), Expect = 1.119e-19 Identity = 56/181 (30.94%), Postives = 89/181 (49.17%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIG--CDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDC 1105
C +C+ +I G + + + SWH CF C C + + + N+ +CV Y + +C C + I T T +Q H F C +C L+G R+ +D+ P C+ C+ YA C C+ I +K +SF++R WH CF C +CS SLV + F T+ +++ C DC
Sbjct: 101 CQECKKTIMPGTRKMEYKGS-----SWHETCFTCQRCQQPIGTKSFIPKENQNFCVPCYEKQYALQCVQCKKPITTGGVT-YRDQPWHRECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 5 Score: 56.225 bits (134), Expect = 3.149e-7 Identity = 38/124 (30.65%), Postives = 51/124 (41.13%), Query Frame = 2
Query: 560 ALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIF---TEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGC 922
AL C QC+ I G + R WH CF CT C + L + PYC+ + L +CA C I +Y E++ H F CK C SL G ++ + D LC C
Sbjct: 159 ALQCVQCKKPITTGGVTYR-------DQPWHRECFVCTACKKQLSGQRFTARDEFPYCLTCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. UniProt/SwissProt
Match: sp|Q14192|FHL2_HUMAN (Four and a half LIM domains protein 2 OS=Homo sapiens OX=9606 GN=FHL2 PE=1 SV=3) HSP 1 Score: 340.502 bits (872), Expect = 1.206e-111 Identity = 150/273 (54.95%), Postives = 204/273 (74.73%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
F C C+ SL G +YIL+++ P C+ C+E+ +AN C++C IGCD KDLS+KDRHWHE CF C +C +SLVD+PFA K++ L C+DCY N+++++C C K G RK EY+G WHE CF+C C+QPIGTKSFIP++ CVPCYE+++A +C +C I GGVTY+ PWHKECF CT C KQL+G +FT++++ YC C+ +L+AKKC+ CT PI+G GG K+ISFE+R WH++CF C KC++SLVG+GFLT D I+CPDCG+
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGK 277 HSP 2 Score: 155.606 bits (392), Expect = 1.766e-41 Identity = 88/277 (31.77%), Postives = 133/277 (48.01%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGEL 1477
F C C+E L Y PYCV + L C C + I + + + H H A F C C NSL + K+DQ LC CY ++Y++ CQ+C I ++ + +K WHE CF C C + + F KDN +C CY+ + A +C C K G YR + WH+ECF+C CR+ + + F R+ C+ C+ + YA+KC C I G ++++ WH +CF C CS L G F ++ + C +C ++
Sbjct: 5 FDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTG--GVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGKDI 279 HSP 3 Score: 115.931 bits (289), Expect = 1.565e-27 Identity = 66/219 (30.14%), Postives = 104/219 (47.49%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
WH CF C++C LVD + ++ C Y+ +C C + I TR ME + H F C C + +I KD+Q C+ CYE Q+A C QC I + ++++++ WH++CF C C L Q F +D+ YC +C+ + +A +C GC G + + +QWH +CF C C + + F+ DI+C C
Sbjct: 61 WHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPI--TTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 4 Score: 91.6633 bits (226), Expect = 3.852e-19 Identity = 57/181 (31.49%), Postives = 88/181 (48.62%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIG--CDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDC 1105
C +C+ +I G + + + SWH CF C C + + + N+ +CV Y + +C C + I T T EQ H F C +C L+G R+ +DD C+ C+ YA C C+ I +K +SF++R WH CF C +CS SLV + F T+ +++ C DC
Sbjct: 101 CQECKKTIMPGTRKMEYKGS-----SWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVT-YREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 5 Score: 54.299 bits (129), Expect = 1.384e-6 Identity = 36/124 (29.03%), Postives = 52/124 (41.94%), Query Frame = 2
Query: 560 ALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIF---TEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGC 922
A+ C QC+ I G + R + WH CF CT C + L + + YC+ + L +CA C I +Y E++ H F CK C SL G ++ + D LC C
Sbjct: 159 AMQCVQCKKPITTGGVTYR-------EQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. UniProt/SwissProt
Match: sp|Q2KI95|FHL2_BOVIN (Four and a half LIM domains protein 2 OS=Bos taurus OX=9913 GN=FHL2 PE=2 SV=1) HSP 1 Score: 337.421 bits (864), Expect = 1.653e-110 Identity = 149/273 (54.58%), Postives = 201/273 (73.63%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
F C C++SL G +Y+L+++QP C+ C+E+ +A+ C++C IGCD KDLS+KDRHWHE CF C C SLVD+PFA K++ L C+DCY ++++RC C K G RK EY+G WHE CF+C C+QPIGTKSFIP++ + CVPCYE +YA +C +C I GGVTY+ PWH+ECF CT C K L+G +FTS++E YC C+ +L+AKKC+ C PI+G GG K+ISFE+R WH++CF C KC++SLVG+GFLT D I+CPDCG+
Sbjct: 5 FDCHHCEDSLFGRKYVLREEQPYCVACFEALFASTCEECGKLIGCDCKDLSYKDRHWHEACFHCSRCRGSLVDKPFAAKEDQLLCTDCYSQEYSSRCQECKKSIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDSENFCVPCYERQYALQCVQCKKPITTGGVTYREQPWHRECFVCTACKKPLSGQRFTSRDEFAYCLGCFCDLYAKKCAGCANPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGK 277 HSP 2 Score: 157.147 bits (396), Expect = 4.294e-42 Identity = 86/273 (31.50%), Postives = 133/273 (48.72%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAEC 1465
F C C++ L Y +PYCV + L C C +LI + + + H H A F C C SL + K+DQ LC CY +Y++ CQ+C I ++ + +K WHE CF C C + + F KD+ +C CY+ ++A +C C K G YR + WH ECF+C C++P+ + F R++ C+ C+ + YA+KC C I G ++++ WH +CF C CS L G F ++ + C +C
Sbjct: 5 FDCHHCEDSLFGRKYVLREEQPYCVACFEALFASTCEECGKLIGCDCKDLSYKDRHWHEACFHCSRCRGSLVDKPFAAKEDQLLCTDCYSQEYSSRCQECKKSIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDSENFCVPCYERQYALQCVQCKKPITTG--GVTYREQPWHRECFVCTACKKPLSGQRFTSRDEFAYCLGCFCDLYAKKCAGCANPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 3 Score: 115.931 bits (289), Expect = 1.990e-27 Identity = 68/219 (31.05%), Postives = 101/219 (46.12%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
WH CF C+ C LVD + ++ C Y+Q RC C + I TR ME + H F C C + +I KD + C+ CYE QYA C QC I + ++++++ WH +CF C C L Q F ++D YC C+ + +A +C GC G + + +QWH +CF C C + + F+ DI+C C
Sbjct: 61 WHEACFHCSRCRGSLVDKPFAAKEDQLLCTDCYSQEYSSRCQECKKSIMPG--TRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDSENFCVPCYERQYALQCVQCKKPI--TTGGVTYREQPWHRECFVCTACKKPLSGQRFTSRDEFAYCLGCFCDLYAKKCAGCANPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 4 Score: 91.2781 bits (225), Expect = 5.159e-19 Identity = 58/181 (32.04%), Postives = 91/181 (50.28%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIG--CDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDC 1105
C +C+ SI G ++E+ + SWH CF C C + + + ++ +CV Y + +C C + I T T EQ H F C +C L+G R+ +D+ C+GC+ YA C C+ I +K +SF++R WH CF C +CS SLV + F T+ +++ C DC
Sbjct: 101 CQECKKSIMPG--TRKMEYKGS---SWHETCFICHRCQQPIGTKSFIPKDSENFCVPCYERQYALQCVQCKKPITTGGVT-YREQPWHRECFVCTACKKPLSGQRFTSRDEFAYCLGCFCDLYAKKCAGCANPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275 HSP 5 Score: 53.1434 bits (126), Expect = 2.988e-6 Identity = 37/124 (29.84%), Postives = 51/124 (41.13%), Query Frame = 2
Query: 560 ALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIF---TEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGC 922
AL C QC+ I G + R + WH CF CT C + L + YC+ + L +CA C I +Y E++ H F CK C SL G ++ + D LC C
Sbjct: 159 ALQCVQCKKPITTGGVTYR-------EQPWHRECFVCTACKKPLSGQRFTSRDEFAYCLGCFCDLYAKKCAGCANPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDC 275
BLAST of Four and A half lim domains vs. UniProt/SwissProt
Match: sp|Q3ZBI6|FHL3_BOVIN (Four and a half LIM domains protein 3 OS=Bos taurus OX=9913 GN=FHL3 PE=2 SV=1) HSP 1 Score: 312.768 bits (800), Expect = 6.900e-101 Identity = 137/273 (50.18%), Postives = 188/273 (68.86%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
F C C SL G +YI DD P C+ CY++ +AN C +C IG DS++L ++DRH+HE CF+C C SL D+PF +D+ L C+DCY + F+++C C + G RK EY G+ WHE CFLC C QP+G++SF+P + CVPCYE K+A +C +C + +GGVTY+ PWH+EC CT C LAG +FTS+E+ PYC C+GELFA KCS C +PITG GG K++SFEDRHWH CF+C +C+ SLVG+GF+ + D+++C C +
Sbjct: 5 FDCAKCSESLYGRKYIQTDDGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNDCYCSAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCQTPLAGQQFTSREDDPYCVTCFGELFAPKCSSCKRPITGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGCSQ 277 HSP 2 Score: 156.377 bits (394), Expect = 1.024e-41 Identity = 92/273 (33.70%), Postives = 122/273 (44.69%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAEC 1465
F C +C E L Y + PYCV Y CA C +LI + E H H F C C SL + +D + LC CY S +++ C C + S+ L + + WHE CF C C L + F YC CY+NKFA RC C K G YR + WH EC +C C+ P+ + F RE D CV C+ E +A KC+ C I G V+++ WH CF+C CS L G F ++ C C
Sbjct: 5 FDCAKCSESLYGRKYIQTDDGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNDCYCSAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQG--GVTYRDQPWHRECLVCTGCQTPLAGQQFTSREDDPYCVTCFGELFAPKCSSCKRPITGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGC 275 HSP 3 Score: 95.1301 bits (235), Expect = 2.378e-20 Identity = 57/183 (31.15%), Postives = 85/183 (46.45%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQC----SGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDC 1105
C+ C ++ G + ++E+ T WH CF C+ C++ L + YCV Y PRCA C + + T+ +Q H C C L G ++ ++D P C+ C+ +A C C +G G K +SF+DRHWH CF C CS+SLV Q F + + C C
Sbjct: 101 CSACGETVMPG--SRKLEYGGQT---WHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTL-TQGGVTYRDQPWHRECLVCTGCQTPLAGQQFTSREDDPYCVTCFGELFAPKCSSCKRPITGLGG--GKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGC 275 HSP 4 Score: 60.077 bits (144), Expect = 1.798e-8 Identity = 34/124 (27.42%), Postives = 54/124 (43.55%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIF---TEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYES 931
C +C ++ G + R WH C CT C L + + PYCV + +L P+C++C I +Y ++ H + F+C C SL G ++ DQ LC GC ++
Sbjct: 162 CARCSKTLTQGGVTYR-------DQPWHRECLVCTGCQTPLAGQQFTSREDDPYCVTCFGELFAPKCSSCKRPITGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGCSQA 278
BLAST of Four and A half lim domains vs. TrEMBL
Match: U6J6Y3 (Four and a half LIM domains protein 2 OS=Echinococcus granulosus OX=6210 GN=EGR_09187 PE=4 SV=1) HSP 1 Score: 695.271 bits (1793), Expect = 0.000e+0 Identity = 332/554 (59.93%), Postives = 411/554 (74.19%), Query Frame = 2
Query: 74 MALKQDIGTPCLDCVDKCRGFKIHGWRKTCLGCDCSVDRHDITDESPLPILEEMKLADKPYYNLFMESLKMARKYGLSWLPIGLQASEVEQF-TSIVGGNIA-SRAGEKLRKQKLRRQIPVHDRCVEKFSHLETPT--------------ERQEAEKFLQFRNNQDLGVGHVYRLDM-AALPCTQCQSSIQAGQLAVRV--EHAANTQM------SWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
MAL+QDIG PCL C +C GFK+H WR TC C CS D+H +TD S L E++L+D + + +AR++GL WLP+G+Q+SEVE F TS+ I A ++R +++R Q+P DR + S L T E +EA +F RN +D G+G V R A + C +C I + VR+ EH + +WHP CF+C+ C E LVD++Y W + KPYC+RHY Q+IRPRCATCD LIF+EEYTRAM+QEHH HFAC+SCD SLTG RYIL+D++P C+ CYE+++AN C+QC +IGCDSKDLSFK+RHWHEKCFKC C++SL D+PFATKD+ LYCSDCYD +FA RCDGC VFKAGMRKYEYRG+QWHEECF+CV+C+QPIG KSFIPR+ +VCVPCYE KYAQ+CTKC VIRRGGVTYKG+PWHKECFTCT C KQLAG+KFTSK+E+PYCA+CYG+LFAKKC+KC KPITGFGGCKFISFEDRHWHSECF C KC+ +LVG+GFLTN+D+I+C DCGR
Sbjct: 1 MALQQDIGKPCLTCASECPGFKLHPWRATCTACHCSYDKHAVTDPSKDSYLHELELSDTTVLKAYDVAQTVAREHGLHWLPVGIQSSEVEAFLTSLPSSEIPRGEAYVEMRTRRIRHQVPPQDR--KPASSLSTAKLNLPPSNTPANLEGESREATRFQNSRNRRDFGIGRVERASAKATVVCAECSEQIGFREFCVRIRPEHRLSDSSDNSYVPAWHPGCFRCSNCSEHLVDFVYAWLNGKPYCLRHYGQMIRPRCATCDHLIFSEEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLYCSDCYDERFAARCDGCQGVFKAGMRKYEYRGQQWHEECFVCVECKQPIGAKSFIPRDNQVVCVPCYEAKYAQRCTKCSEVIRRGGVTYKGNPWHKECFTCTSCGKQLAGLKFTSKDEQPYCADCYGDLFAKKCTKCGKPITGFGGCKFISFEDRHWHSECFACSKCDCNLVGRGFLTNEDQIMCSDCGR 552
BLAST of Four and A half lim domains vs. TrEMBL
Match: A0A0X3PEW6 (Four and a half LIM domains protein 2 OS=Schistocephalus solidus OX=70667 GN=FHL2 PE=4 SV=1) HSP 1 Score: 695.271 bits (1793), Expect = 0.000e+0 Identity = 336/555 (60.54%), Postives = 411/555 (74.05%), Query Frame = 2
Query: 74 MALKQDIGTPCLDCVDKCRGFKIHGWRKTCLGCDCSVDRHDITDESPLPILEEMKLADKPYYNLFMESLKMARKYGLSWLPIGLQASEVEQFTSIVGGNIASR--AGEKLRKQKLRRQIPVHDR---CVEKFSHLETPT---------ERQEAEKFLQFRNNQDLGVGHVYRL-DMAALPCTQCQSSIQAGQLAVRV--EH---AANTQM---SWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSK---DLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
MA +QDIG CL C +C GF +H WR TC GC CS D+HD+T+ S ++E++L+D + + A + GL+WLP+GL +SE++ F + + + R A LR++++RRQIP DR L P E +EA +F RN +D G+G V R+ D A LPC +C +I VRV EH AN +WHP CF+C+ C E LVD+ Y W + KPYC+RHY Q+IRPRCATCD LIF+EEYTRAM+QEHH HFAC+SCD SLTG RYIL+D++P C+ CYE++YAN C+QC +IGCDSK DLSFK+RHWHEKCFKC CS+SL D+PFATK++ LYCSDCYD +FA RCDGC VFKAGMRKYEYRG+QWHEECF+CV+C+QPIG KSFIPRE +VCVPCYE KYAQ+CTKC VIRRGGVTYKG+PWHKECFTCT C KQLAG+KFTSK+E+PYCA+CYGELFAKKC+KC+KPITGFGGCKFISFEDRHWHSECF C KCN +LVG+GFLTN+D+I+C DCGR
Sbjct: 1 MAFQQDIGKSCLTCGTECSGFSLHAWRATCTGCHCSYDKHDVTNFSHESFIKELELSDTTLLASYEVAETTAAENGLTWLPVGLSSSEIDAFIASLPSSEVPRGEANAALRQKRIRRQIPPQDRKPAASLSAVKLNLPPDAAAGNLDGEIREATRFQNSRNRRDFGLGRVDRVTDKAGLPCAECSETIGFRDFCVRVRPEHRPAEANDSEHAPAWHPGCFRCSNCTEHLVDFAYAWLNGKPYCLRHYGQMIRPRCATCDHLIFSEEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKYANTCEQCKEKIGCDSKSPPDLSFKERHWHEKCFKCSTCSTSLADRPFATKEDQLYCSDCYDERFAARCDGCQGVFKAGMRKYEYRGQQWHEECFVCVECKQPIGAKSFIPRENQVVCVPCYEAKYAQRCTKCTEVIRRGGVTYKGNPWHKECFTCTSCGKQLAGLKFTSKDEQPYCADCYGELFAKKCTKCSKPITGFGGCKFISFEDRHWHSECFACSKCNCNLVGRGFLTNEDQIMCSDCGR 555
BLAST of Four and A half lim domains vs. TrEMBL
Match: A0A068Y1Z9 (Four and A half lim domains OS=Echinococcus multilocularis OX=6211 PE=4 SV=1) HSP 1 Score: 693.73 bits (1789), Expect = 0.000e+0 Identity = 332/554 (59.93%), Postives = 411/554 (74.19%), Query Frame = 2
Query: 74 MALKQDIGTPCLDCVDKCRGFKIHGWRKTCLGCDCSVDRHDITDESPLPILEEMKLADKPYYNLFMESLKMARKYGLSWLPIGLQASEVEQF-TSIVGGNIA-SRAGEKLRKQKLRRQIPVHDRCVEKFSHLETPT--------------ERQEAEKFLQFRNNQDLGVGHVYRLDM-AALPCTQCQSSIQAGQLAVRV--EHAANTQM------SWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
MAL+QDIG PCL C +C GFK+H WR TC C CS D+H +TD S L E++L+D + + +AR++GL WLP+G+Q+SEVE F TS+ I A ++R +++R Q+P DR + S L T E +EA +F RN +D G+G V R A + C +C I + VR+ EH + +WHP CF+C+ C E LVD++Y W + KPYC+RHY Q+IRPRCATCD LIF+EEYTRAM+QEHH HFAC+SCD SLTG RYIL+D++P C+ CYE+++AN C+QC +IGCDSKDLSFK+RHWHEKCFKC C++SL D+PFATKD+ LYCSDCYD +FA RCDGC VFKAGMRKYEYRG+QWHEECF+CV+C+QPIG KSFIPR+ +VCVPCYE KYAQ+CTKC VIRRGGVTYKG+PWHKECFTCT C KQLAG+KFTSK+E+PYCA+CYG+LFAKKC+KC KPITGFGGCKFISFEDRHWHSECF C KC+ +LVG+GFLTN+D+I+C DCGR
Sbjct: 1 MALQQDIGKPCLTCGSECPGFKLHPWRATCTACHCSYDKHAVTDPSKDSYLHELELSDTTVLKAYDVAQTIAREHGLHWLPVGIQSSEVEAFLTSLPSSEIPRGEAYVEMRTRRIRHQVPPQDR--KPASSLSTAKLNLPPSNTPANLEGESREATRFQNSRNRRDFGIGRVERASAKATVVCAECSEQIGFREFCVRIRPEHRLSDSSDNSYVPAWHPGCFRCSNCSEHLVDFVYAWLNGKPYCLRHYGQMIRPRCATCDHLIFSEEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLYCSDCYDERFAARCDGCQGVFKAGMRKYEYRGQQWHEECFVCVECKQPIGAKSFIPRDNQVVCVPCYEAKYAQRCTKCSEVIRRGGVTYKGNPWHKECFTCTSCGKQLAGLKFTSKDEQPYCADCYGDLFAKKCTKCGKPITGFGGCKFISFEDRHWHSECFACSKCDCNLVGRGFLTNEDQIMCSDCGR 552
BLAST of Four and A half lim domains vs. TrEMBL
Match: A0A0R3X1E4 (Uncharacterized protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS7003 PE=4 SV=1) HSP 1 Score: 693.345 bits (1788), Expect = 0.000e+0 Identity = 331/554 (59.75%), Postives = 414/554 (74.73%), Query Frame = 2
Query: 74 MALKQDIGTPCLDCVDKCRGFKIHGWRKTCLGCDCSVDRHDITDESPLPILEEMKLADKPYYNLFMESLKMARKYGLSWLPIGLQASEVEQFTSIVGGNIASRAG--EKLRKQKLRRQIPVHDRCVEKFSHLETPT--------------ERQEAEKFLQFRNNQDLGVGHVYRLDM-AALPCTQCQSSIQAGQLAVRV--EH----AANTQMS--WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
MAL+QD+G PCL C +C GFK+H WR TC GC CS D+H +TD S L E++L+D + + +A+++GL WLP+G+Q++EVE F + + + R ++R +++R Q+P DR + S L T E +EA +F RN +D G+G V R+ A + C +C I + VRV EH +++T + WHP CF+C+ C E LVD +Y W + KPYC+RHY Q+IRPRCATCD LIF+EEYTRAM+QEHH HFAC+SCD SLTG RYIL+D++P C+ CYE+++AN C+QC +IGCDSKDLSFK+RHWHEKCFKC C++SL D+PFATKD+ LYCSDCYD +FA RCDGC VFKAGMRKYEYRG+QWHEECF+CV+C+QPIG KSFIPR+ +VCVPCYE KYAQ+CTKC VIRRGGVTYKG+PWHKECFTCT C KQLAG+KFTSK+E+PYCA+CYGELFAKKC+KC KPITGFGGCKFISFEDRHWHSECF C KC+ +LVG+GFLTNDD+I+C DCGR
Sbjct: 1 MALQQDVGKPCLTCGSECSGFKLHPWRATCTGCHCSYDKHAVTDPSKDSYLHELELSDVTVLKAYDVAQAIAKEHGLHWLPVGIQSAEVEAFLTSLPSSEIPRGEVYAEMRFRRIRHQVPPQDR--KPASSLSTAKLNLPPSTAPANLEGESREATRFQNSRNRRDFGIGRVERVSAKATIVCAECSEHIGFREFCVRVRPEHRLSDSSDTSYAPAWHPGCFRCSNCSEHLVDLVYGWLNGKPYCLRHYGQMIRPRCATCDHLIFSEEYTRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLYCSDCYDERFAARCDGCQGVFKAGMRKYEYRGQQWHEECFVCVECKQPIGAKSFIPRDNQVVCVPCYEAKYAQRCTKCAEVIRRGGVTYKGNPWHKECFTCTSCGKQLAGLKFTSKDEQPYCADCYGELFAKKCTKCGKPITGFGGCKFISFEDRHWHSECFACSKCDCNLVGRGFLTNDDQIMCSDCGR 552
BLAST of Four and A half lim domains vs. TrEMBL
Match: A0A0R3T6D5 (Uncharacterized protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS2622 PE=4 SV=1) HSP 1 Score: 692.96 bits (1787), Expect = 0.000e+0 Identity = 324/550 (58.91%), Postives = 410/550 (74.55%), Query Frame = 2
Query: 74 MALKQDIGTPCLDCVDKCRGFKIHGWRKTCLGCDCSVDRHDITDESPLPILEEMKLADKPYYNLFMESLKMARKYGLSWLPIGLQASEVEQFTSIVGGNIASRAG--EKLRKQKLRRQIPVHDR------------CVEKFSHLETPTERQEAEKFLQFRNNQDLGVGHVYRLDMAA-LPCTQCQSSIQAGQLAV------RVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
MAL QD+G PCLDC +C GFK+H WR +C C CS ++H IT+ S L E++L D F + +A+++ L+WLPIG+QASE+E F + + N R ++R +++R Q+P DR V +H+ E +EA +F RN +D G+G + R + C +C I +L V R+ ++A+ SWHP CF+C C E LVD++YCW + KPYC+RHY QL+RPRC+TCD LIF+EEY RAM+QEHH HFAC+SCD SLTG RYIL+D++P C+ CYE+++AN C+QC +IGCDSKDLSFK+RHWHEKCFKC C++SL D+PFATKD+ L+CSDCYD +FA RCDGC VFKAGMRKYEYRG+QWHEECF+CV+C+QPIG KSFIPRE +VCVPCYE+KYAQ+C+KC VIRRGGVTYKG+PWHKECFTCT C KQLAG+KFTSK+E+PYCA+C+G+LFAKKC+KC KPITGFGGCKFISFEDRHWHSECF C KCN +LVG+GFLTN+D+I+C DCGR
Sbjct: 1 MALPQDVGKPCLDCGSECPGFKLHPWRASCNNCLCSFNKHAITESSGTTYLHELELPDTALIKAFEAAQAIAKEHELNWLPIGIQASEIEAFLASLPANELPRGDTYAQMRLKRIRSQVPPQDRKPSASLNTAKLNLVSSDAHVNLDGEIREATRFQVNRNKRDFGIGRIDRSSTNPNIACFECSEPIGFHELRVHVRQEHRLANSADDVPSWHPGCFRCATCCEHLVDFVYCWLNGKPYCLRHYGQLVRPRCSTCDHLIFSEEYIRAMDQEHHTGHFACRSCDVSLTGQRYILRDEEPHCLACYEAKFANTCEQCKEKIGCDSKDLSFKERHWHEKCFKCSACNTSLADRPFATKDDQLFCSDCYDERFAARCDGCQGVFKAGMRKYEYRGQQWHEECFVCVECKQPIGAKSFIPRENQVVCVPCYEQKYAQRCSKCSEVIRRGGVTYKGNPWHKECFTCTSCGKQLAGLKFTSKDEQPYCADCFGDLFAKKCTKCGKPITGFGGCKFISFEDRHWHSECFACSKCNANLVGRGFLTNEDQIMCSDCGR 550
BLAST of Four and A half lim domains vs. Ensembl Cavefish
Match: fhl2a (four and a half LIM domains protein 2 [Source:NCBI gene;Acc:103031012]) HSP 1 Score: 336.265 bits (861), Expect = 6.067e-111 Identity = 147/273 (53.85%), Postives = 202/273 (73.99%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
+ C C SL G +Y+L+++ P C+ CYES Y+N C++C IGC+S+DLS+KDRHWHE+CF CF+C SLVD+PF+TKD L C++CY N+++++C C K G RK E++G WHE CF C C+QPIGTKSFIP++ + CVPCYE+++A +C C I GGVTY PWHK+CF CT C +QL+G +FTS+++ YC C+ L+AKKC+ CT PI+G GG K+ISFE+R WH++CF C KC++SLVG+GFLT D I+CP+CG+
Sbjct: 5 YDCHYCKESLFGKKYVLREENPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEECFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCKRCQQPIGTKSFIPKDNNNYCVPCYEKQFAMQCVHCKKPITTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPECGK 277 HSP 2 Score: 153.68 bits (387), Expect = 8.724e-42 Identity = 89/273 (32.60%), Postives = 126/273 (46.15%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAEC 1465
+ C C E L Y PYCV+ Y L C C + I + + H H F C C SL + KD+Q LC CY ++Y++ C +C I S+ + K WHE CF C C + + F KDNN YC CY+ +FA +C C K G Y + WH++CFLC C+Q + + F R+ C+ C+ YA+KC C I G ++++ WH +CF C CS L G F ++ + C EC
Sbjct: 5 YDCHYCKESLFGKKYVLREENPYCVKCYESLYSNTCEECKKPIGCNSRDLSYKDRHWHEECFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGNSWHETCFTCKRCQQPIGTKSFIPKDNNNYCVPCYEKQFAMQCVHCKKPITTG--GVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275 HSP 3 Score: 112.849 bits (281), Expect = 2.700e-27 Identity = 65/219 (29.68%), Postives = 100/219 (45.66%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
WH CF C +C LVD + + C Y+ +C C + I +R ME + + H F CK C + +I KD+ C+ CYE Q+A C C I + +++ D+ WH+ CF C C L Q F ++D+ YC +C+ N +A +C C G + + +QWH +CF C C + + F+ DI+C C
Sbjct: 61 WHEECFHCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPG--SRKMEHKGNSWHETCFTCKRCQQPIGTKSFIPKDNNNYCVPCYEKQFAMQCVHCKKPI--TTGGVTYHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275 HSP 4 Score: 96.2857 bits (238), Expect = 1.078e-21 Identity = 60/181 (33.15%), Postives = 90/181 (49.72%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIG--CDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDC 1105
C +C+ +I G + ++EH N SWH CF C C + + + N YCV Y + +C C + I T T +Q H F C C L+G R+ +DD C+ C+ + YA C C+ I SK +SF++R WH CF C +CS SLV + F T+ +++ C +C
Sbjct: 101 CHECKKTIMPG--SRKMEHKGN---SWHETCFTCKRCQQPIGTKSFIPKDNNNYCVPCYEKQFAMQCVHCKKPITTGGVT-YHDQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275 HSP 5 Score: 50.447 bits (119), Expect = 2.852e-6 Identity = 34/124 (27.42%), Postives = 50/124 (40.32%), Query Frame = 2
Query: 560 ALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIF---TEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGC 922
A+ C C+ I G + WH CF CT C + L + + YC+ + L +CA+C I +Y E++ H F CK C SL G ++ + D LC C
Sbjct: 159 AMQCVHCKKPITTGGVTYH-------DQPWHKDCFLCTGCKQQLSGQRFTSRDDFAYCLNCFCNLYAKKCASCTSPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275
BLAST of Four and A half lim domains vs. Ensembl Cavefish
Match: fhl3a (four and a half LIM domains 3 [Source:NCBI gene;Acc:103023824]) HSP 1 Score: 328.561 bits (841), Expect = 4.548e-108 Identity = 149/272 (54.78%), Postives = 194/272 (71.32%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCG 1657
F C +C SL G +YI +D P CI CY+S +AN C +C IG D+++L ++DRH+HE CF+CF C SL D+PF ++D+ L C+DCY N+F+++C C K G RK EY G WHE CF+C C QPIG+KSFIP + D CVPCYE K+A +CT+C V+ +GGVTY+ PWHKECF CT C QLAG FTS+++ PYC +C+G L+AKKC C+KPITGFGG K+ISFE+R WH CFTC +C++SLVG GF D+I+C DC
Sbjct: 5 FDCDNCKESLYGRKYIQAEDNPYCIPCYDSLFANTCDECKELIGHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDDALLCNDCYCNEFSSKCVACDKTVMPGTRKLEYGGSTWHEGCFICNSCEQPIGSKSFIPDKDDHYCVPCYENKFAPRCTRCKQVLAKGGVTYRDEPWHKECFLCTGCKVQLAGQHFTSRDDSPYCLKCFGNLYAKKCEACSKPITGFGGGKYISFEERQWHQTCFTCSRCSVSLVGAGFFPERDEILCRDCN 276 HSP 2 Score: 157.147 bits (396), Expect = 5.438e-43 Identity = 90/277 (32.49%), Postives = 130/277 (46.93%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH-FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGEL 1477
F C C E L Y + PYC+ Y L C C ELI + E H+ H F C CD SL + +DD LC CY +++++ C C + ++ L + WHE CF C C + + F ++ YC CY+NKFA RC C +V G YR + WH+ECFLC C+ + + F R+ C+ C+ YA+KC C I G ++++ WH+ CFTC+ CS L G F + ++ C +C L
Sbjct: 5 FDCDNCKESLYGRKYIQAEDNPYCIPCYDSLFANTCDECKELIGHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDDALLCNDCYCNEFSSKCVACDKTVMPGTRKLEYGGSTWHEGCFICNSCEQPIGSKSFIPDKDDHYCVPCYENKFAPRCTRCKQVLAKG--GVTYRDEPWHKECFLCTGCKVQLAGQHFTSRDDSPYCLKCFGNLYAKKCEACSKPITGFGGGKYISFEERQWHQTCFTCSRCSVSLVGAGFFPERDEILCRDCNSNL 279 HSP 3 Score: 112.464 bits (280), Expect = 3.739e-27 Identity = 62/219 (28.31%), Postives = 100/219 (45.66%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAME---QEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
+H CF+C CD L D + + C Y +C CD+ + TR +E H F C SC+ + +I D C+ CYE+++A C +C + ++++D WH++CF C C L Q F ++D++ YC C+ N +A +C+ C K G + + +QWH+ CF C C + F P +I+C C
Sbjct: 61 YHEHCFRCFRCDRSLADEPFTSQDDALLCNDCYCNEFSSKCVACDKTVMPG--TRKLEYGGSTWHEGCFICNSCEQPIGSKSFIPDKDDHYCVPCYENKFAPRCTRCKQVLA--KGGVTYRDEPWHKECFLCTGCKVQLAGQHFTSRDDSPYCLKCFGNLYAKKCEACSKPITGFGGGKYISFEERQWHQTCFTCSRCSVSLVGAGFFPERDEILCRDC 275 HSP 4 Score: 92.4337 bits (228), Expect = 2.213e-20 Identity = 57/184 (30.98%), Postives = 84/184 (45.65%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIG--CDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDN 1114
C C ++ G ++E+ +T WH CF C C++ + + + YCV Y PRC C +++ T E H F C C L G + +DD P C+ C+ + YA C+ CS I K +SF++R WH+ CF C CS SLV F + + + C DC N
Sbjct: 101 CVACDKTVMPG--TRKLEYGGST---WHEGCFICNSCEQPIGSKSFIPDKDDHYCVPCYENKFAPRCTRCKQVLAKGGVTYRDEPWHKEC-FLCTGCKVQLAGQHFTSRDDSPYCLKCFGNLYAKKCEACSKPITGFGGGKYISFEERQWHQTCFTCSRCSVSLVGAGFFPERDEILCRDCNSN 278
BLAST of Four and A half lim domains vs. Ensembl Cavefish
Match: fhl2b (four and a half LIM domains 2b [Source:ZFIN;Acc:ZDB-GENE-041010-121]) HSP 1 Score: 279.641 bits (714), Expect = 2.394e-89 Identity = 131/273 (47.99%), Postives = 185/273 (67.77%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
+ C C SL G +Y+L+++ P C+ CYES Y+N C++C I C+S+DLS+KDRHWH+ CF CF+C+ SLVD+PF+TK+ L C++CY N+++++C C K G RK E++G WHE CF C C+QPIGTKSFIP++ CVPC+E+++A +C +C V T +CF CT C +QL+G +FTS+++ PYC C+ L+AKKCS CT PI+G GG K+ISFE+R WH++CF C KC+I D I+CPDCG+
Sbjct: 5 YDCHYCKESLFGKKYVLREENPYCVKCYESLYSNTCEECKKPICCNSRDLSYKDRHWHDDCFHCFKCNRSLVDKPFSTKNEELLCTECYSNEYSSKCYECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNANYCVPCFEKQFALQCVQCKKVHTVYNST--------DCFLCTGCKEQLSGQRFTSRDDFPYCLNCFCNLYAKKCSSCTSPISGLGGSKYISFEERQWHNDCFNCKKCSI-----------DDILCPDCGK 258 HSP 2 Score: 120.939 bits (302), Expect = 2.317e-30 Identity = 75/253 (29.64%), Postives = 113/253 (44.66%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCS 1405
+ C C E L Y PYCV+ Y L C C + I + + H H F C C+ SL + K+++ LC CY ++Y++ C +C I S+ + K WHE CF C C + + F KDN YC C++ +FA +C C KV +CFLC C++ + + F R+ C+ C+ YA+KC+ C I G ++++ WH +CF C CS
Sbjct: 5 YDCHYCKESLFGKKYVLREENPYCVKCYESLYSNTCEECKKPICCNSRDLSYKDRHWHDDCFHCFKCNRSLVDKPFSTKNEELLCTECYSNEYSSKCYECKKTIMPGSRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNANYCVPCFEKQFALQCVQCKKVHTVYNST----------DCFLCTGCKEQLSGQRFTSRDDFPYCLNCFCNLYAKKCSSCTSPISGLGGSKYISFEERQWHNDCFNCKKCS 247 HSP 3 Score: 82.8037 bits (203), Expect = 3.254e-17 Identity = 55/198 (27.78%), Postives = 85/198 (42.93%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDC 1225
WH CF C +C+ LVD + + + C Y+ +C C + I +R ME + + H F C+ C + +I KD+ C+ C+E Q+A C QC + CF C C L Q F ++D+ YC +C+ N +A +C C G + + +QWH +CF C C
Sbjct: 61 WHDDCFHCFKCNRSLVDKPFSTKNEELLCTECYSNEYSSKCYECKKTIMPG--SRKMEHKGNSWHETCFTCQRCQQPIGTKSFIPKDNANYCVPCFEKQFALQCVQCK----------KVHTVYNSTDCFLCTGCKEQLSGQRFTSRDDFPYCLNCFCNLYAKKCSSCTSPISGLGGSKYISFEERQWHNDCFNCKKC 246
BLAST of Four and A half lim domains vs. Ensembl Cavefish
Match: fhl1a (four and a half LIM domains 1 [Source:NCBI gene;Acc:103045707]) HSP 1 Score: 256.529 bits (654), Expect = 5.954e-80 Identity = 122/275 (44.36%), Postives = 172/275 (62.55%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNN-LYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFG-GCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
F C C ++L G +Y+ KDD+ +C+ C+E AN C +C IG D+K+L+ K+RHWHE CF+C +C L ++ FA +D+ + C C + + RC GC+KV G + EY+ K WHEECF C +C+QPI + SF+ + D C PC+E+K+A+ C +C I G+TY+ PWH ECF C C K LAG +FT+ E+ YC +CY AKKCS C PITGFG G +++E+ WH CF C KC++SL K F+ N + I C DC +
Sbjct: 21 FDCFYCRDNLQGKKYVNKDDKHVCVRCFEKLCANTCAECRRPIGVDAKELTHKNRHWHEDCFRCAKCYKPLANESFAARDDGKVMCGKCGAREDSARCQGCYKVIMPGDKNVEYKHKVWHEECFTCFECKQPIRSLSFLTKGDDFYCTPCHEKKFAKNCARCKEPITANGITYQDKPWHSECFVCQTCRKPLAGGRFTAHEDYFYCVDCYKTSVAKKCSGCQNPITGFGKGTNVVNYENNTWHEYCFNCKKCSLSLAHKRFVLNGENIYCTDCAK 295 HSP 2 Score: 143.665 bits (361), Expect = 5.987e-38 Identity = 91/279 (32.62%), Postives = 128/279 (45.88%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDD-QPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG-----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGEL 1477
F C C + L Y +K CVR + +L CA C I + + H H F C C L + +DD + +C C + + CQ C I K++ +K + WHE+CF CFEC + F TK ++ YC+ C++ KFA C C + A Y+ K WH ECF+C CR+P+ F E CV CY+ A+KC+ C I G V Y+ + WH+ CF C CS LA +F E YC +C +L
Sbjct: 21 FDCFYCRDNLQGKKYVNKDDKHVCVRCFEKLCANTCAECRRPIGVDAKELTHKNRHWHEDCFRCAKCYKPLANESFAARDDGKVMCGKCGAREDSARCQGCYKVIMPGDKNVEYKHKVWHEECFTCFECKQPIRSLSFLTKGDDFYCTPCHEKKFAKNCARCKEPITAN--GITYQDKPWHSECFVCQTCRKPLAGGRFTAHEDYFYCVDCYKTSVAKKCSGCQNPITGFGKGTNVVNYENNTWHEYCFNCKKCSLSLAHKRFVLNGENIYCTDCAKKL 297 HSP 3 Score: 102.834 bits (255), Expect = 8.947e-24 Identity = 67/252 (26.59%), Postives = 107/252 (42.46%), Query Frame = 2
Query: 554 MAALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSN-KPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKV---FKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
+ A C +C+ I V + + WH CF+C +C + L + + + K C + A+ RC C ++I + + +E +H H F C C + ++ K D C C+E ++A C +C I + ++++D+ WH +CF C C L F ++ YC DCY A +C GC F G Y WHE CF C C + K F+ ++I C C
Sbjct: 51 LCANTCAECRRPI-----GVDAKELTHKNRHWHEDCFRCAKCYKPLANESFAARDDGKVMCGKCGAREDSARCQGCYKVIMPGD--KNVEYKHKVWHEECFTCFECKQPIRSLSFLTKGDDFYCTPCHEKKFAKNCARCKEPI--TANGITYQDKPWHSECFVCQTCRKPLAGGRFTAHEDYFYCVDCYKTSVAKKCSGCQNPITGFGKGTNVVNYENNTWHEYCFNCKKCSLSLAHKRFVLNGENIYCTDC 293 HSP 4 Score: 66.6254 bits (161), Expect = 1.609e-11 Identity = 39/108 (36.11%), Postives = 57/108 (52.78%), Query Frame = 2
Query: 1337 RRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDD-KIICPDCG 1657
R G S E F C +C L G K+ +K++K C C+ +L A C++C +PI G K ++ ++RHWH +CF C KC L + F DD K++C CG
Sbjct: 5 RHSGPRSYLSSTMTERFDCFYCRDNLQGKKYVNKDDKHVCVRCFEKLCANTCAECRRPI-GVDA-KELTHKNRHWHEDCFRCAKCYKPLANESFAARDDGKVMCGKCG 110
BLAST of Four and A half lim domains vs. Ensembl Cavefish
Match: fhl1a (four and a half LIM domains 1 [Source:NCBI gene;Acc:103045707]) HSP 1 Score: 255.758 bits (652), Expect = 7.038e-80 Identity = 122/275 (44.36%), Postives = 172/275 (62.55%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNN-LYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFG-GCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
F C C ++L G +Y+ KDD+ +C+ C+E AN C +C IG D+K+L+ K+RHWHE CF+C +C L ++ FA +D+ + C C + + RC GC+KV G + EY+ K WHEECF C +C+QPI + SF+ + D C PC+E+K+A+ C +C I G+TY+ PWH ECF C C K LAG +FT+ E+ YC +CY AKKCS C PITGFG G +++E+ WH CF C KC++SL K F+ N + I C DC +
Sbjct: 5 FDCFYCRDNLQGKKYVNKDDKHVCVRCFEKLCANTCAECRRPIGVDAKELTHKNRHWHEDCFRCAKCYKPLANESFAARDDGKVMCGKCGAREDSARCQGCYKVIMPGDKNVEYKHKVWHEECFTCFECKQPIRSLSFLTKGDDFYCTPCHEKKFAKNCARCKEPITANGITYQDKPWHSECFVCQTCRKPLAGGRFTAHEDYFYCVDCYKTSVAKKCSGCQNPITGFGKGTNVVNYENNTWHEYCFNCKKCSLSLAHKRFVLNGENIYCTDCAK 279 HSP 2 Score: 143.28 bits (360), Expect = 5.006e-38 Identity = 91/279 (32.62%), Postives = 128/279 (45.88%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDD-QPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG-----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGEL 1477
F C C + L Y +K CVR + +L CA C I + + H H F C C L + +DD + +C C + + CQ C I K++ +K + WHE+CF CFEC + F TK ++ YC+ C++ KFA C C + A Y+ K WH ECF+C CR+P+ F E CV CY+ A+KC+ C I G V Y+ + WH+ CF C CS LA +F E YC +C +L
Sbjct: 5 FDCFYCRDNLQGKKYVNKDDKHVCVRCFEKLCANTCAECRRPIGVDAKELTHKNRHWHEDCFRCAKCYKPLANESFAARDDGKVMCGKCGAREDSARCQGCYKVIMPGDKNVEYKHKVWHEECFTCFECKQPIRSLSFLTKGDDFYCTPCHEKKFAKNCARCKEPITAN--GITYQDKPWHSECFVCQTCRKPLAGGRFTAHEDYFYCVDCYKTSVAKKCSGCQNPITGFGKGTNVVNYENNTWHEYCFNCKKCSLSLAHKRFVLNGENIYCTDCAKKL 281 HSP 3 Score: 102.834 bits (255), Expect = 7.967e-24 Identity = 67/252 (26.59%), Postives = 107/252 (42.46%), Query Frame = 2
Query: 554 MAALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSN-KPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKV---FKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
+ A C +C+ I V + + WH CF+C +C + L + + + K C + A+ RC C ++I + + +E +H H F C C + ++ K D C C+E ++A C +C I + ++++D+ WH +CF C C L F ++ YC DCY A +C GC F G Y WHE CF C C + K F+ ++I C C
Sbjct: 35 LCANTCAECRRPI-----GVDAKELTHKNRHWHEDCFRCAKCYKPLANESFAARDDGKVMCGKCGAREDSARCQGCYKVIMPGD--KNVEYKHKVWHEECFTCFECKQPIRSLSFLTKGDDFYCTPCHEKKFAKNCARCKEPI--TANGITYQDKPWHSECFVCQTCRKPLAGGRFTAHEDYFYCVDCYKTSVAKKCSGCQNPITGFGKGTNVVNYENNTWHEYCFNCKKCSLSLAHKRFVLNGENIYCTDC 277
BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Match: fhl5 (four and a half LIM domains 5 [Source:ZFIN;Acc:ZDB-GENE-040822-3]) HSP 1 Score: 192.586 bits (488), Expect = 5.954e-58 Identity = 87/170 (51.18%), Postives = 116/170 (68.24%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK-AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG 1348
+ C C SL G +Y+L+++ P C+ CYE+ Y + C+ C IGCDSKDLS+KDRHWHE CFKC C SL D+ F+ +D L C++C+ N ++ +C G K+F G RK E+RG WHE CF C C+QPIG+KSF+PRE+D CVPCYE+++AQ CT C V GG
Sbjct: 13 YDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAGISKLFSPVGTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKKVGGEGG 182 HSP 2 Score: 105.531 bits (262), Expect = 1.767e-26 Identity = 50/171 (29.24%), Postives = 86/171 (50.29%), Query Frame = 2
Query: 1025 FKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKC---TKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGG 1528
+ C C SL+ + + ++ N YC CY+ + C+ C + + Y+ + WHE CF C C + + K+F R++ ++C C+ Y+ KC +K + + ++GS WH+ CF C C + + F +E+ YC CY + FA+ C+ C K + G GG
Sbjct: 13 YDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAGISKLFSPVGTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKK-VGGEGG 182 HSP 3 Score: 85.8853 bits (211), Expect = 1.362e-19 Identity = 49/169 (28.99%), Postives = 76/169 (44.97%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIG-CDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAG 1162
+ C C E L+ Y PYCVR Y L C +C + I + + + H H F C +C+ SL + +D++ LC C+ + Y+ C S ++ + F+ WHE CF C C + + F ++ + YC CY+ +FA C C KV G
Sbjct: 13 YDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAGISKLFSPVGTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKKVGGEG 181 HSP 4 Score: 82.4185 bits (202), Expect = 1.873e-18 Identity = 46/151 (30.46%), Postives = 73/151 (48.34%), Query Frame = 2
Query: 1208 FLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVI--RRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDC 1654
+ C C++ + K ++ RE++ CV CYE Y C C I ++YK WH+ CF C C + LA F++++EK C EC+ ++ KC+ +K + G K + F WH CF C +C + K F+ + C C
Sbjct: 13 YDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAGISKLFSPVGTRK-MEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPC 162 HSP 5 Score: 53.9138 bits (128), Expect = 1.405e-8 Identity = 32/107 (29.91%), Postives = 45/107 (42.06%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAME---QEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQC 958
WH CFKC C+ L D + K C +A +CA +L F+ TR ME H F C C + ++ ++ C+ CYE Q+A C C
Sbjct: 69 WHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAGISKL-FSPVGTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLC 174
BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Match: fhl5 (four and a half LIM domains 5 [Source:ZFIN;Acc:ZDB-GENE-040822-3]) HSP 1 Score: 187.578 bits (475), Expect = 6.013e-56 Identity = 85/171 (49.71%), Postives = 113/171 (66.08%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVT 1354
+ C C SL G +Y+L+++ P C+ CYE+ Y + C+ C IGCDSKDLS+KDRHWHE CFKC C SL D+ F+ +D L C++C+ N ++ +C G RK E+RG WHE CF C C+QPIG+KSF+PRE+D CVPCYE+++AQ CT C V GG T
Sbjct: 6 YDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAA--NPNPVGTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKKVGGEGGPT 174 HSP 2 Score: 108.612 bits (270), Expect = 1.932e-27 Identity = 49/168 (29.17%), Postives = 84/168 (50.00%), Query Frame = 2
Query: 1025 FKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGG 1528
+ C C SL+ + + ++ N YC CY+ + C+ C + + Y+ + WHE CF C C + + K+F R++ ++C C+ Y+ KC + + ++GS WH+ CF C C + + F +E+ YC CY + FA+ C+ C K + G GG
Sbjct: 6 YDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAANPNPVGTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKK-VGGEGG 172 HSP 3 Score: 88.1965 bits (217), Expect = 2.087e-20 Identity = 49/168 (29.17%), Postives = 77/168 (45.83%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAG 1162
+ C C E L+ Y PYCVR Y L C +C + I + + + H H F C +C+ SL + +D++ LC C+ + Y+ C +G ++ + F+ WHE CF C C + + F ++ + YC CY+ +FA C C KV G
Sbjct: 6 YDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAANPNPVG--TRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLCKKVGGEG 171 HSP 4 Score: 83.5741 bits (205), Expect = 9.061e-19 Identity = 45/151 (29.80%), Postives = 72/151 (47.68%), Query Frame = 2
Query: 1208 FLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVI--RRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDC 1654
+ C C++ + K ++ RE++ CV CYE Y C C I ++YK WH+ CF C C + LA F++++EK C EC+ ++ KC+ P+ G + + F WH CF C +C + K F+ + C C
Sbjct: 6 YDCHYCKESLLGKKYVLREENPYCVRCYETLYTHACESCKQPIGCDSKDLSYKDRHWHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAANPNPV----GTRKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPC 152 HSP 5 Score: 48.1358 bits (113), Expect = 1.215e-6 Identity = 30/107 (28.04%), Postives = 42/107 (39.25%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAME---QEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQC 958
WH CFKC C+ L D + K C +A +CA + T R ME H F C C + ++ ++ C+ CYE Q+A C C
Sbjct: 62 WHEGCFKCATCERSLADKAFSARDEKLLCTECHANHYSAKCAANPNPVGT----RKMEFRGSSWHETCFGCARCQQPIGSKSFVPREKDQYCVPCYEKQFAQHCTLC 164
BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Match: fhl3b (four and a half LIM domains 3b [Source:ZFIN;Acc:ZDB-GENE-030131-8356]) HSP 1 Score: 181.8 bits (460), Expect = 2.537e-53 Identity = 86/227 (37.89%), Postives = 125/227 (55.07%), Query Frame = 2
Query: 989 LSFKDRHWHEKCFKCFECSSSL-VDQPFATKDNNLYCSDCYDN---KFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCG 1657
LS + HWHE CFKC EC++ + + + + C C + C C + + G+ K Y+G+ WHE CF C C + IGTKSF+P +I CV C E+K A +C C + GV YK +H+EC C C+ LAG+ ++ E YC C+ + ++KKC+ C KPIT G KF++ + WH+ECF C +C +++ GKGF+ D I C DC
Sbjct: 2 LSLDNLHWHEGCFKCSECATPIGAATEYLLEGKEIVCKTCATSPAGDMGDSCVSCKRGIREGVAKVAYKGRTWHERCFACARCMETIGTKSFVPHGDEIYCVSCNEKKTATRCFTCHKPVTEKGVVYKEHTYHRECLVCIGCNTPLAGMNMCTRGELLYCEPCFEKKYSKKCTSCRKPITSAAGAKFLTHDSLEWHTECFKCKRCGLNMAGKGFVARGDDIFCGDCA 228 HSP 2 Score: 120.168 bits (300), Expect = 4.378e-31 Identity = 65/224 (29.02%), Postives = 105/224 (46.88%), Query Frame = 2
Query: 830 HPAHFACKSCDNSL-TGHRYILKDDQPLCIGCYESQYANI---CQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGEL 1477
H F C C + Y+L+ + +C C S ++ C C I +++K R WHE+CF C C ++ + F + +YC C + K ATRC CHK + Y+ +H EC +C+ C P+ + R + + C PC+E+KY++KCT C I +T+ WH ECF C C +AG F ++ + +C +C ++
Sbjct: 10 HEGCFKCSECATPIGAATEYLLEGKEIVCKTCATSPAGDMGDSCVSCKRGIREGVAKVAYKGRTWHERCFACARCMETIGTKSFVPHGDEIYCVSCNEKKTATRCFTCHKPVTE--KGVVYKEHTYHRECLVCIGCNTPLAGMNMCTRGELLYCEPCFEKKYSKKCTSCRKPITSAAGAKFLTHDSLEWHTECFKCKRCGLNMAGKGFVARGDDIFCGDCANQI 231 HSP 3 Score: 90.5077 bits (223), Expect = 8.271e-21 Identity = 59/227 (25.99%), Postives = 93/227 (40.97%), Query Frame = 2
Query: 638 QMSWHPRCFKCTECDEVLVDYIYCWYSNKPY----CVRHYAQLIRPRCATCDELIFTEEYTRAME-QEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVF--KAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEE 1297
+ WH CFKC+EC + K C A + C +C I A + + H FAC C ++ ++ D+ C+ C E + A C C + K + +K+ +H +C C C++ L T+ LYC C++ K++ +C C K AG + + +WH ECF C C + K F+ R DI C C +
Sbjct: 6 NLHWHEGCFKCSECATPIGAATEYLLEGKEIVCKTCATSPAGDMGDSCVSCKRGIREGVAKVAYKGRTWHERCFACARCMETIGTKSFVPHGDEIYCVSCNEKKTATRCFTCHKPV--TEKGVVYKEHTYHRECLVCIGCNTPLAGMNMCTRGELLYCEPCFEKKYSKKCTSCRKPITSAAGAKFLTHDSLEWHTECFKCKRCGLNMAGKGFVARGDDIFCGDCANQ 230 HSP 4 Score: 82.8037 bits (203), Expect = 3.620e-18 Identity = 49/183 (26.78%), Postives = 80/183 (43.72%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRI--GCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYD 1111
C C+ I+ G V A +WH RCF C C E + + + ++ YCV + RC TC + + TE+ E +H C C+ L G + + C C+E +Y+ C C I +K L+ WH +CFKC C ++ + F + ++++C DC +
Sbjct: 53 CVSCKRGIREG-----VAKVAYKGRTWHERCFACARCMETIGTKSFVPHGDEIYCVSCNEKKTATRCFTCHKPV-TEKGVVYKEHTYHRECLVCIGCNTPLAGMNMCTRGELLYCEPCFEKKYSKKCTSCRKPITSAAGAKFLTHDSLEWHTECFKCKRCGLNMAGKGFVARGDDIFCGDCAN 229
BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Match: tes (testis derived transcript (3 LIM domains) [Source:ZFIN;Acc:ZDB-GENE-040718-59]) HSP 1 Score: 153.68 bits (387), Expect = 2.290e-41 Identity = 118/380 (31.05%), Postives = 165/380 (43.42%), Query Frame = 2
Query: 152 RKTCLGCDCSVDRHDITDESPLPILEEMKLADKPYYNLF------MESLKMARKYGLSWLPIG----LQASEVEQFTSIVGGNIASR----------------AGEKLRKQKLRRQIPVHDRCVEKFSHLETPTERQEAEKFLQFRNNQDLGVGHVYRL------------------------DMAALP-----CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDE----LIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEK--CFKCFECSSSLVDQPFATKDNNLYCS-DC 1105
RK C C C ++ HDI E D Y + ME+ + ++ LS I L + GN +R G R+++L RQ+P HD+ + L + E ++ E+F++ + LGVG V D AA C +C+ +Q G AV E A + + WHP CF C+ CDE+LVD +Y W K YC RHY RPRC CDE LIF++EYT+A EQ H HF C CD L G Y+++ D+P+C CY +YA CQ C G I +++ ++ + WH CF C C LV F ++CS DC
Sbjct: 1 RKICRNCKCGLEEHDIQMEEEAQRKVGRLFEDSRYAGVADRIRSDMETRLLEQQPRLSNHLIARKNRLGGTLTVSTAEPADGNRTARRYIELLPRDKQPIEGSQGAMYRRRQLARQLPEHDQDPSRCHDL-SAAEVKQMEEFVKKYKAEALGVGDVMLPHQVGGSAAFAQAAGEASAGERGVGDGAAQAQRDYRCNRCKQPMQQGDPAVFAERAGSEHL-WHPGCFCCSACDEILVDLVYFWKRGKLYCGRHYCDTERPRCGGCDEVWTQLIFSKEYTQAEEQNWHLKHFCCFECDCVLAGEIYVMEQDKPVCKPCYMKKYARSCQSCQGSIEPEAQRVTLGEFSWHADPTCFLCSCCGIPLVGLRFLPHQAMIFCSADC 378 HSP 2 Score: 81.2629 bits (199), Expect = 1.069e-16 Identity = 53/165 (32.12%), Postives = 77/165 (46.67%), Query Frame = 2
Query: 1010 WHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEY---RGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG--VTYKGSPWHKE--CFTCTHCSKQLAGIKFTSKEEKPYC-AECYGELF 1480
WH CF C C LVD + K LYC Y + RC GC +V+ + EY + WH + F C +C + + ++ + VC PCY +KYA+ C C G I VT WH + CF C+ C L G++F + +C A+C G++
Sbjct: 219 WHPGCFCCSACDEILVDLVYFWKRGKLYCGRHYCDTERPRCGGCDEVWTQLIFSKEYTQAEEQNWHLKHFCCFECDCVLAGEIYVMEQDKPVCKPCYMKKYARSCQSCQGSIEPEAQRVTLGEFSWHADPTCFLCSCCGIPLVGLRFLPHQAMIFCSADCRGKVV 383 HSP 3 Score: 58.151 bits (139), Expect = 2.524e-9 Identity = 42/158 (26.58%), Postives = 66/158 (41.77%), Query Frame = 2
Query: 1193 WHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGV-----IRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSE--CFTCYKCNISLVGKGFLTNDDKIIC 1645
WH CF C C + + + + + C Y + +C C V + + WH + F C C LAG + +++KP C CY + +A+ C C I + ++ + WH++ CF C C I LVG FL + I C
Sbjct: 219 WHPGCFCCSACDEILVDLVYFWKRGKLYCGRHYCDTERPRCGGCDEVWTQLIFSKEYTQAEEQNWHLKHFCCFECDCVLAGEIYVMEQDKPVCKPCYMKKYARSCQSCQGSIEPEA--QRVTLGEFSWHADPTCFLCSCCGIPLVGLRFLPHQAMIFC 374
BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Match: PXN (paxillin [Source:HGNC Symbol;Acc:HGNC:9718]) HSP 1 Score: 118.242 bits (295), Expect = 1.824e-30 Identity = 70/229 (30.57%), Postives = 102/229 (44.54%), Query Frame = 2
Query: 800 EYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKY-EYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFA 1483
+ AM + HP HF C C + + KD QP C Y S ++ C C+ + + + ++ DR WH + F C +C ++ F +D YC Y FA +C+GC +A M+ Y WH ECF+C +C P SF E C Y E+ C+ C I ++ +H E F C C KQL F + +KPYC C+ +LF
Sbjct: 1 QVVTAMGRTWHPEHFVCVHCQVEIGSRNFFEKDGQPYCEEDYHSLFSPRCAYCNEAVMDNQRVVTALDRTWHPEHFFCAQCGRPFGEEGFHERDGKPYCRQDYYGMFAPKCNGCS---RAIMQNYLSALNTVWHPECFICRECFTPFINGSFFEHEGQPYCEVHYHERRGSLCSGCQKPITGRCISAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQPCFIKLFG 226 HSP 2 Score: 110.538 bits (275), Expect = 8.734e-28 Identity = 69/217 (31.80%), Postives = 91/217 (41.94%), Query Frame = 2
Query: 1004 RHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDC 1654
R WH + F C C + + F KD YC + Y + F+ RC C++ R + WH E F C C +P G + F R+ C Y +A KC C I + ++ + WH ECF C C F E +PYC Y E CS C KPITG + IS + +H E F C C L F +DK C C
Sbjct: 8 RTWHPEHFVCVHCQVEIGSRNFFEKDGQPYCEEDYHSLFSPRCAYCNEAVMDNQRVVTALDRTWHPEHFFCAQCGRPFGEEGFHERDGKPYCRQDYYGMFAPKCNGCSRAIMQNYLSALNTVWHPECFICRECFTPFINGSFFEHEGQPYCEVHYHERRGSLCSGCQKPITG----RCISAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQPC 220 HSP 3 Score: 99.3673 bits (246), Expect = 6.282e-24 Identity = 61/217 (28.11%), Postives = 91/217 (41.94%), Query Frame = 2
Query: 644 SWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIF-TEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCY 1291
+WHP F C C + + +PYC Y L PRCA C+E + + A+++ HP HF C C + +D +P C Y +A C CS I LS + WH +CF C EC + ++ F + YC Y + + C GC K R K++H E F+C C + + +F + C PC+
Sbjct: 9 TWHPEHFVCVHCQVEIGSRNFFEKDGQPYCEEDYHSLFSPRCAYCNEAVMDNQRVVTALDRTWHPEHFFCAQCGRPFGEEGFHERDGKPYCRQDYYGMFAPKCNGCSRAI--MQNYLSALNTVWHPECFICRECFTPFINGSFFEHEGQPYCEVHYHERRGSLCSGCQKPITG--RCISAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQPCF 221 HSP 4 Score: 65.855 bits (159), Expect = 2.069e-12 Identity = 34/99 (34.34%), Postives = 48/99 (48.48%), Query Frame = 2
Query: 1349 VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIIC 1645
VT G WH E F C HC ++ F K+ +PYC E Y LF+ +C+ C + + + ++ DR WH E F C +C +GF D K C
Sbjct: 3 VTAMGRTWHPEHFVCVHCQVEIGSRNFFEKDGQPYCEEDYHSLFSPRCAYCNEAVM--DNQRVVTALDRTWHPEHFFCAQCGRPFGEEGFHERDGKPYC 99
BLAST of Four and A half lim domains vs. Ensembl Yeast
Match: LRG1 (GTPase-activating protein (GAP); contains Rho1p-specific GAP activity, interacting with activated forms of Rho1p; functions along with Sac7p as a negative regulator of the Pkc1p-mediated cell wall integrity signaling pathway; negative regulator of cell wall 1,3-beta-glucan biosynthesis; required for efficient cell fusion; contains a RhoGAP domain and three Lin-11-Isl1-Mec-3 (LIM) domains [Source:SGD;Acc:S000002399]) HSP 1 Score: 50.447 bits (119), Expect = 7.236e-7 Identity = 44/145 (30.34%), Postives = 69/145 (47.59%), Query Frame = 2
Query: 1184 GKQWHEECFLCVDCRQPIGTKSFIPREQD------IVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISF---EDRH-WHSECFTCYK 1588
GK +HE CF C DC++P+ K F P + D ++C Y ++ C C +R T G + +E F+CT C+ K + YC + + F+K+C C PI+ ++I F E+ H WH EC+ +K
Sbjct: 50 GKYYHESCFTCQDCQKPLKPKYF-PYQVDKTSESILLCQYDYFRRHNLLCHVCDTPLRGLYYTAFGYRYDEEHFSCTICATPCGVKKCFMYGNQLYCKYHFLKYFSKRCKGCEFPISD----QYIEFPKGEEIHCWHPECYGIHK 189
BLAST of Four and A half lim domains vs. Ensembl Nematostella
Match: LIMPET (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFX6]) HSP 1 Score: 362.844 bits (930), Expect = 5.648e-118 Identity = 195/529 (36.86%), Postives = 292/529 (55.20%), Query Frame = 2
Query: 86 QDI-GTPCLDCVDKCRGFKIHGWRKTCLGCDCSVDRHDI-TDESPLPILEE-MKLADKPYYNLFMESLKMARKYGLSWLPIGLQASEVEQFTSIVGGNIASRA---GEKLRKQKLRRQIPVHDRCVEKFSHLETPTERQEAEKFLQFRNNQDLGVGHVYRLDMAALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDC 1654
+DI G CL C + CRGF +H WRK C C C + H + T+ + EE M + ++ L L+ A G W+P GL + E++ + S++ + + G + R ++L QIP+ D + L T ++ + F + R LGVG V C +C + G +A+ E WH +CF C E E+LVD IYC ++ YC RH+ + ++PRCA C+ELI+ EY++A+E+ HP H C CD SL+ +++ + P C CY+ +AN C+ C IG SKD+ + +HWHE CFKC +CS L+++ F KD L C C + C C+ F G +K Y+ K +H++CF+C +C+QPIG+K FI R++ +C C++ K+A+ C KC VI+ V + GS +H ECFTC HC K LAG FT +E + C CY E +AK+C C I G KF++++++++H ECFTC KCN L G+ F D + IC C
Sbjct: 27 EDIEGNKCLQCGNICRGFSVHPWRKFCNSCKCPHELHVLSTNNNDQNTPEERMGVEEEDPPMLQGSHLQQAMMEGYEWVPRGLTSEEIQIYMSLLPEDKIPKLKSLGAQHRTKQLSLQIPLQDFSSKHCKKL-TLDQKMAMDDFCEKRIKTALGVGAVRACMAQPSTCKRCNGPVNPGDMALFAERLG-PDSCWHVKCFTCEEDGELLVDLIYCSKDDEIYCCRHWGEKLKPRCAGCEELIYVGEYSQALEKNWHPGHLCCSYCDESLSNQKFVTVEGSPSCFRCYDENFANRCEACGEPIGPGSKDVDVRSKHWHEGCFKCSQCSKQLMNEGFTLKDEKLICHGCRGINPSKVCAACNGDFAPGEKKVGYQSKTFHDKCFICDECKQPIGSKQFIRRDERRLCNNCFDSKFAKVCVKCNQVIKTSSVQHAGSTYHSECFTCHHCDKPLAGSPFTKQEGRNVCQNCYRERYAKRCGACHNLIE--GNTKFVAYDEKYFHRECFTCCKCNKPLAGEKFRIRDGEKICLPC 551 HSP 2 Score: 116.701 bits (291), Expect = 1.951e-27 Identity = 68/246 (27.64%), Postives = 113/246 (45.93%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPR--CATCD-ELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEE 1297
C C I G V V WH CFKC++C + L++ + K C H + I P CA C+ + E+ + H F C C + ++I +D++ LC C++S++A +C +C+ I S + +H +CF C C L PF ++ C +CY ++A RC CH + + + Y K +H ECF C C +P+ + F R+ + +C+PC ++
Sbjct: 318 CEACGEPIGPGSKDVDVR-----SKHWHEGCFKCSQCSKQLMNEGFTLKDEKLIC--HGCRGINPSKVCAACNGDFAPGEKKVGYQSKTFHDKCFICDECKQPIGSKQFIRRDERRLCNNCFDSKFAKVCVKCNQVIKTSS--VQHAGSTYHSECFTCHHCDKPLAGSPFTKQEGRNVCQNCYRERYAKRCGACHNLIEGNTKFVAYDEKYFHRECFTCCKCNKPLAGEKFRIRDGEKICLPCDDD 554
BLAST of Four and A half lim domains vs. Ensembl Nematostella
Match: EDO41100 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S5D2]) HSP 1 Score: 355.91 bits (912), Expect = 1.460e-115 Identity = 196/539 (36.36%), Postives = 291/539 (53.99%), Query Frame = 2
Query: 74 MALKQDI--GTPCLDCVDKCRGFKIHGWRKTCLGCDCSVDRHDITDES--PLPILEEMKLADKPYYNLFMESLKMARKYGLSWLPIGLQASEVEQFTSIVGGN---IASRAGEKLRKQKLRRQIPVHDRCVEKFSHLETPTERQEAEKFLQFRNNQDLGVGH-VYRLDMAALP-CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKD-RHWHEKCFKCFECSSSLVDQ-PFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCG 1657
M L QDI G PCL C D+C GF +H W+K C C C + HDI +E+ + I + D P + KY W P GL S+VE + + I G K + ++ Q+PV DR EK S E F+QF ++ D G V ++ C +C I + AV E+ + S+HP CF C C+E+LV+ Y +++K YC RH+A+L + RC CDELIFT EYT AM + H HF C++CD+S+TG ++I++ D+P+C C++ YA+ C+ C +IG +S+D+S D RHWH+KCF C C L + + + C+ CY + C C ++ +G + EY G WHE CF C +C + IGT F+P++ C CY+ K++++C C + GGV Y G WHK CF+C C + LA F+ ++ YC ECYG+ +AK+C C K I G ++ + E+ ++H ECF C +C SL +GF+ D+++C DC
Sbjct: 1 MILLQDIDEGKPCLKCKDRCPGFSLHVWKKACQYCKCPREDHDIQNEANETMDINTRINGIDLPPPPKVISG--GFPKY--LWTPSGLSQSQVELYMKELPPQKVPIKDSPGSKYYRAQMAYQLPVQDRDPEKLSTFSA----AHRESFMQFNSSADNARGEGVVKVPFGRRQRCARCSHPILRSEPAVVAENIG-AEASFHPGCFTCETCNELLVELTYFQHADKVYCGRHFAELQKSRCGGCDELIFTGEYTVAMNKNWHLGHFQCQTCDHSITGRQFIVRGDKPVCTDCFKDSYAHECEACHQKIGPESRDISSDDDRHWHDKCFICDICRRPLKSEGSLHSTETKSCCNKCYVANYQKECCACGQIIDSGASRLEYSGNFWHENCFRCANCGEAIGTSGFVPKDDTFFCPGCYQSKFSKRCASCGEPLLEGGVLYNGETWHKACFSCYFCHRSLASAAFSVRDGCRYCMECYGKFYAKQCEICLKAIVGG---EYYTLEESNFHKECFMCSRCGRSLASEGFVREGDELLCGDCA 527 HSP 2 Score: 75.8702 bits (185), Expect = 2.051e-14 Identity = 52/181 (28.73%), Postives = 81/181 (44.75%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYD 1111
C C I +G A R+E++ N WH CF+C C E + + + +C Y RCA+C E + E + H A F+C C SL + ++D C+ CY YA C+ C I + + ++ ++H++CF C C SL + F + + L C DC D
Sbjct: 355 CCACGQIIDSG--ASRLEYSGNF---WHENCFRCANCGEAIGTSGFVPKDDTFFCPGCYQSKFSKRCASCGEPLL-EGGVLYNGETWHKACFSCYFCHRSLASAAFSVRDGCRYCMECYGKFYAKQCEICLKAI-VGGEYYTLEESNFHKECFMCSRCGRSLASEGFVREGDELLCGDCAD 528
BLAST of Four and A half lim domains vs. Ensembl Nematostella
Match: EDO49801 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFY0]) HSP 1 Score: 209.149 bits (531), Expect = 2.526e-61 Identity = 120/364 (32.97%), Postives = 182/364 (50.00%), Query Frame = 2
Query: 68 Y*MALKQDIGTPCLDCVDKCRGFKIHGWRKTCLGCDCSVDRHDITD------ESPLPILEEMKLAD-KPYYNLFMESLKMARK---YGLSWLPIGLQASEVEQFTSIVGGN---IASRAGEKLRKQKLRRQIPVHDRCVEKFSHLETPTERQEAEKFLQFRNNQDLGVGHVYRLDMAA--LPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWH--EKCFKCFECSSSLVDQPFATKDNNLYCS-DC 1105
Y + +++ G CL C DKC G +H WRK C C C + HD+ + E + ++ +A + Y+N + + +W+P G+ + ++ + I G K R+ ++ Q+P+HD +L + ERQ+ E F RN LGVG + AA C +C + G++AV A + WHP CF CT C+ +LVD IY + YC RHYA+ +PRCA CDELIF+E YT+A ++ H HF C CD L G Y+ + QP C+ CY+ YA C C I D+K + + + WH +CF C +C+ ++ + F NN++CS DC
Sbjct: 19 YKLGHERNEGAKCLKCGDKCEGLDLHFWRKICKNCKCKQEDHDLYEDIANEHEVKSRLFKDSNVAHLRDYFNKLTAANDQPKTEFVKKFTWVPPGMNQGILVKYMESLPKELVPIVGSEGAKYRRAQMVYQLPIHDHDPNYCDNL-SDAERQKMEDFCAMRNQDALGVGDIREKTQAASKWNCFRCSKPVMTGEVAVFASRAGEDK-CWHPGCFVCTVCNNLLVDLIYFYKDGVIYCGRHYAEQFKPRCAACDELIFSETYTQAEDRNWHQRHFCCLECDRDLGGQLYVARGGQPHCLECYDKYYAKHCMSCKKNIAADAKRIEHQGQFWHATSECFHCAKCNKDMLGKQFLKTKNNIFCSVDC 380 HSP 2 Score: 88.9669 bits (219), Expect = 5.114e-19 Identity = 52/160 (32.50%), Postives = 77/160 (48.12%), Query Frame = 2
Query: 1193 WHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTY-KGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWH--SECFTCYKCNISLVGKGFLTNDDKIICP-DCGR 1660
WH CF+C C + + ++ I C Y E++ +C C +I T + WH+ F C C + L G + ++ +P+C ECY + +AK C C K I K I + + WH SECF C KCN ++GK FL + I C DC +
Sbjct: 225 WHPGCFVCTVCNNLLVDLIYFYKDGVIYCGRHYAEQFKPRCAACDELIFSETYTQAEDRNWHQRHFCCLECDRDLGGQLYVARGGQPHCLECYDKYYAKHCMSCKKNIA--ADAKRIEHQGQFWHATSECFHCAKCNKDMLGKQFLKTKNNIFCSVDCAK 382
BLAST of Four and A half lim domains vs. Ensembl Nematostella
Match: EDO49798 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFX7]) HSP 1 Score: 194.512 bits (493), Expect = 3.407e-56 Identity = 115/356 (32.30%), Postives = 171/356 (48.03%), Query Frame = 2
Query: 86 QDIGTPCLDCVDKCRGFKIHGWRKTCLGCDCSVDRHDITDESPLPILEEMKLADKPYYNLFMESLKMARKYG-----------LSWLPIGLQASEVEQFTSIVGGN---IASRAGEKLRKQKLRRQIPVHDRCVEKFSHLETPTERQEAEKFLQFRNNQDLGVGHVYRL-DMA-ALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWH--EKCFKCFECSSSLVDQPFATKDNNLYCS 1099
D C +C D C GF H WRK C C C + H+I + + N++M + A +W+P GL +V + + + GE+ R +++ Q+P HD +F + + E++E F+ R LG G V ++ +M+ C +C + AG +AV A WH CF CT C E+LVD IY + +K YC RH+A+ ++PRCA CDE+IF E+ T A + H HF C CD L G RY+++D++P C C+ES YA C C I D+ ++ +HWH +CF C C +L+ PF K +YCS
Sbjct: 7 DDGSKACNNCGDTCPGFSAHYWRKICRNCKCPREDHNIISDDA-----------RAGRNIYMADVHRASSLASDDDSGCALDEYAWVPPGLGPEQVHSYMNALPEEKVPYVDSIGERYRNRQIILQLPPHDN-EARFCNGLSEEEKRELCFFVALRKRDSLGRGVVKQVPEMSEGYSCKECGECVTAGSMAVYASRAGQ-HTCWHASCFICTTCKELLVDLIYFYKDSKVYCGRHHAETLKPRCAACDEIIFAEQCTEAEDSCWHVQHFCCFECDCPLGGMRYVMRDNKPYCCHCFESLYAEFCDSCGEPIEPDASQMAHNGQHWHATNECFSCCTCGKALLGLPFLPKSGEIYCS 349 HSP 2 Score: 87.4261 bits (215), Expect = 1.256e-18 Identity = 56/183 (30.60%), Postives = 87/183 (47.54%), Query Frame = 2
Query: 1130 CDGCHKVFKAG-MRKYEYRGKQ---WHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVT-YKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHS--ECFTCYKCNISLVGKGFLTNDDKIIC-PDC 1654
C C + AG M Y R Q WH CF+C C++ + + ++ + C + E +C C +I T + S WH + F C C L G+++ ++ KPYC C+ L+A+ C C +PI ++ +HWH+ ECF+C C +L+G FL +I C PDC
Sbjct: 172 CKECGECVTAGSMAVYASRAGQHTCWHASCFICTTCKELLVDLIYFYKDSKVYCGRHHAETLKPRCAACDEIIFAEQCTEAEDSCWHVQHFCCFECDCPLGGMRYVMRDNKPYCCHCFESLYAEFCDSCGEPIEPDA--SQMAHNGQHWHATNECFSCCTCGKALLGLPFLPKSGEIYCSPDC 352 HSP 3 Score: 82.0333 bits (201), Expect = 7.750e-17 Identity = 49/154 (31.82%), Postives = 70/154 (45.45%), Query Frame = 2
Query: 1010 WHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG--VTYKGSPWH--KECFTCTHCSKQLAGIKFTSKEEKPYCA 1459
WH CF C C LVD + KD+ +YC + RC C ++ A + E WH + F C +C P+G ++ R+ C C+E YA+ C C I + + G WH ECF+C C K L G+ F K + YC+
Sbjct: 197 WHASCFICTTCKELLVDLIYFYKDSKVYCGRHHAETLKPRCAACDEIIFA-EQCTEAEDSCWHVQHFCCFECDCPLGGMRYVMRDNKPYCCHCFESLYAEFCDSCGEPIEPDASQMAHNGQHWHATNECFSCCTCGKALLGLPFLPKSGEIYCS 349
BLAST of Four and A half lim domains vs. Ensembl Nematostella
Match: EDO41181 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S548]) HSP 1 Score: 121.709 bits (304), Expect = 5.190e-31 Identity = 80/240 (33.33%), Postives = 110/240 (45.83%), Query Frame = 2
Query: 767 CATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKY-EYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFA 1483
CA C++ I + T A+ + HP HFAC +C+ L + +D +P C Y +A C C+G I DS ++ D+ WH + F C EC + D F +D +C + Y FA RC GC + M Y WH ECF+C +CRQP SF + C Y K C C I +T +H E F C C KQL F + +KPYC C+ +LF
Sbjct: 26 CAACNKPIIGQVCT-ALGKTWHPEHFACVACEAPLGTQNFFERDGKPYCERDYHDTFAPRCAYCNGPI-LDSC-VTALDQTWHPEHFVCAECGNPFGDTGFHERDGKPFCREDYYAMFAPRCGGCGQPI---MDNYISALSAHWHAECFICTECRQPFPGGSFFDHDGRPYCEMHYHAKRGTLCYSCQKPITGRCITAMHRKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCHPCFVKLFG 259 HSP 2 Score: 108.227 bits (269), Expect = 2.248e-26 Identity = 55/154 (35.71%), Postives = 73/154 (47.40%), Query Frame = 2
Query: 1184 GKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIIC 1645
GK WH E F CV C P+GT++F R+ C Y + +A +C C G I VT WH E F C C F ++ KP+C E Y +FA +C C +PI +IS HWH+ECF C +C G F +D + C
Sbjct: 42 GKTWHPEHFACVACEAPLGTQNFFERDGKPYCERDYHDTFAPRCAYCNGPILDSCVTALDQTWHPEHFVCAECGNPFGDTGFHERDGKPFCREDYYAMFAPRCGGCGQPIMD----NYISALSAHWHAECFICTECRQPFPGGSFFDHDGRPYC 191 HSP 3 Score: 103.99 bits (258), Expect = 7.087e-25 Identity = 67/216 (31.02%), Postives = 92/216 (42.59%), Query Frame = 2
Query: 1010 WHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCH-KVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDC 1654
WH + F C C + L Q F +D YC Y + FA RC C+ + + + + + WH E F+C +C P G F R+ C Y +A +C C I ++ + WH ECF CT C + G F + +PYC Y C C KPITG + I+ R +H E F C C L F +DK C C
Sbjct: 45 WHPEHFACVACEAPLGTQNFFERDGKPYCERDYHDTFAPRCAYCNGPILDSCVTALD---QTWHPEHFVCAECGNPFGDTGFHERDGKPFCREDYYAMFAPRCGGCGQPIMDNYISALSAHWHAECFICTECRQPFPGGSFFDHDGRPYCEMHYHAKRGTLCYSCQKPITG----RCITAMHRKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCHPC 253 HSP 4 Score: 101.679 bits (252), Expect = 4.340e-24 Identity = 66/221 (29.86%), Postives = 90/221 (40.72%), Query Frame = 2
Query: 644 SWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYA 1306
+WHP F C C+ L + KPYC R Y PRCA C+ I T A++Q HP HF C C N + +D +P C Y + +A C C I +S HWH +CF C EC F D YC Y K T C C K R +++H E F+C C + + +F + C PC+ + +
Sbjct: 44 TWHPEHFACVACEAPLGTQNFFERDGKPYCERDYHDTFAPRCAYCNGPILDSCVT-ALDQTWHPEHFVCAECGNPFGDTGFHERDGKPFCREDYYAMFAPRCGGCGQPI--MDNYISALSAHWHAECFICTECRQPFPGGSFFDHDGRPYCEMHYHAKRGTLCYSCQKPITG--RCITAMHRKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCHPCFVKLFG 259 HSP 5 Score: 60.8474 bits (146), Expect = 3.298e-10 Identity = 36/111 (32.43%), Postives = 45/111 (40.54%), Query Frame = 2
Query: 1313 CTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIIC 1645
C C I T G WH E F C C L F ++ KPYC Y + FA +C+ C PI ++ D+ WH E F C +C GF D K C
Sbjct: 26 CAACNKPIIGQVCTALGKTWHPEHFACVACEAPLGTQNFFERDGKPYCERDYHDTFAPRCAYCNGPILD----SCVTALDQTWHPEHFVCAECGNPFGDTGFHERDGKPFC 132
BLAST of Four and A half lim domains vs. Ensembl Medaka
Match: fhl2a (four and a half LIM domains protein 2 [Source:NCBI gene;Acc:101169645]) HSP 1 Score: 337.421 bits (864), Expect = 1.710e-111 Identity = 148/273 (54.21%), Postives = 202/273 (73.99%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
+ C C SL G +Y+L+++ P C+ CYES Y+N C++C IGC+++DLS+KDRHWHE CFKCF+C SLVD+PF+TKD L C++CY N+++++C C K G RK E++G WHE CF C C+QPIGTKSFIP++ CVPCYE+++A +C C I GGVTY+ PWHK+CF CT C +QL+G +FTSK++ YC C+ L+AKKC+ CT PI+G GG K+ISFE+R WH++CF C KC++SLVG+GFLT D I+CP+CG+
Sbjct: 5 YDCHYCKESLFGKKYVLREENPYCVKCYESLYSNTCEECKKPIGCNTRDLSYKDRHWHEDCFKCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGHSWHETCFTCKRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTGGVTYRDQPWHKDCFLCTSCKQQLSGQRFTSKDDFAYCLNCFCNLYAKKCASCTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPECGK 277 HSP 2 Score: 152.14 bits (383), Expect = 3.548e-41 Identity = 88/273 (32.23%), Postives = 127/273 (46.52%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAEC 1465
+ C C E L Y PYCV+ Y L C C + I + + H H F C C SL + KD+Q LC CY ++Y++ C +C I S+ + K WHE CF C C + + F KDN+ YC CY+ +FA +C C K G YR + WH++CFLC C+Q + + F ++ C+ C+ YA+KC C I G ++++ WH +CF C CS L G F ++ + C EC
Sbjct: 5 YDCHYCKESLFGKKYVLREENPYCVKCYESLYSNTCEECKKPIGCNTRDLSYKDRHWHEDCFKCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPGSRKMEHKGHSWHETCFTCKRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPITTG--GVTYRDQPWHKDCFLCTSCKQQLSGQRFTSKDDFAYCLNCFCNLYAKKCASCTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275 HSP 3 Score: 119.398 bits (298), Expect = 1.419e-29 Identity = 74/265 (27.92%), Postives = 113/265 (42.64%), Query Frame = 2
Query: 530 VGHVYRLDMAALPCTQCQSSIQAGQ-------LAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
G Y L C +C S+ + + + WH CFKC +C LVD + + C Y+ +C C + I +R ME + H H F CK C + +I KD+ C+ CYE Q+A C C I + ++++D+ WH+ CF C C L Q F +KD+ YC +C+ N +A +C C G + + +QWH +CF C C + + F+ DI+C C
Sbjct: 15 FGKKYVLREENPYCVKCYESLYSNTCEECKKPIGCNTRDLSYKDRHWHEDCFKCFQCKRSLVDKPFSTKDEQLLCTECYSNEYSSKCHECKKTIMPG--SRKMEHKGHSWHETCFTCKRCQQPIGTKSFIPKDNHNYCVPCYEKQFAMQCVHCKKPI--TTGGVTYRDQPWHKDCFLCTSCKQQLSGQRFTSKDDFAYCLNCFCNLYAKKCASCTTPISGLGGSKYISFEERQWHNDCFNCKKCSVSLVGRGFLTERDDILCPEC 275
BLAST of Four and A half lim domains vs. Ensembl Medaka
Match: fhl3a (four and a half LIM domains 3 [Source:NCBI gene;Acc:101174533]) HSP 1 Score: 336.265 bits (861), Expect = 2.615e-110 Identity = 150/284 (52.82%), Postives = 200/284 (70.42%), Query Frame = 2
Query: 806 TRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCG 1657
++A+++ F C +C SL G +YI +D P CI CY+S ++N C +C IG D+++L ++DRH+HE CF+CF C SL D+PF ++DN L C+DCY N+F+++C C K+ G RK EY G WHE CF+C C QPIG+KSFIP + + CVPCYE+K+A +CT+C + +GGVTY+ PWHKECF CT C QLAG FTS++E PYC +C+ L+AKKC C KPITGFGG K+ISFEDR WH CFTC +C++SLVG GF N D+I+C DC
Sbjct: 40 SQALKKTTMADRFDCDNCKESLYGRKYIQSNDSPYCIPCYDSLFSNTCDECKELIGHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDNALLCNDCYCNEFSSKCVACDKIVMPGTRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALSKGGVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPITGFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDCN 323 HSP 2 Score: 154.836 bits (390), Expect = 1.067e-41 Identity = 87/277 (31.41%), Postives = 128/277 (46.21%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH-FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGEL 1477
F C C E L Y ++ PYC+ Y L C C ELI + E H+ H F C CD SL + +D+ LC CY +++++ C C + ++ L + WHE CF C C + + F + YC CY++KFA RC C K G YR + WH+ECF+C C + + F R++ C+ C+ YA+KC C I G ++++ WH+ CFTC+ CS L G F ++ C +C L
Sbjct: 52 FDCDNCKESLYGRKYIQSNDSPYCIPCYDSLFSNTCDECKELIGHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDNALLCNDCYCNEFSSKCVACDKIVMPGTRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALSKG--GVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPITGFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDCNSNL 326 HSP 3 Score: 113.62 bits (283), Expect = 3.617e-27 Identity = 62/219 (28.31%), Postives = 100/219 (45.66%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAME---QEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
+H CF+C CD L D + N C Y +C CD+++ TR +E H F C SC+ + +I D+ C+ CYE ++A C +C + ++++D WH++CF C C + L Q F ++D + YC C+ + +A +C+ C K G + + +QWH+ CF C C + F P I+C C
Sbjct: 108 YHEHCFRCFRCDRSLADEPFTSQDNALLCNDCYCNEFSSKCVACDKIVMPG--TRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALS--KGGVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPITGFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDC 322 HSP 4 Score: 94.7449 bits (234), Expect = 7.490e-21 Identity = 59/184 (32.07%), Postives = 85/184 (46.20%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGC--DSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDN 1114
C C + G ++E+A +T WH CF C C++ + + ++ YCV Y PRC C + + T E H F C SC+ L G + +D+ P C+ C+ S YA C+ C I K +SF+DR WH+ CF C +CS SLV F + + C DC N
Sbjct: 148 CVACDKIVMPG--TRKLEYAGST---WHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALSKGGVTYRDEPWHKEC-FVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPITGFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDCNSN 325
BLAST of Four and A half lim domains vs. Ensembl Medaka
Match: fhl5 (four and a half LIM domains protein 2-like [Source:NCBI gene;Acc:101156370]) HSP 1 Score: 332.028 bits (850), Expect = 2.537e-109 Identity = 150/273 (54.95%), Postives = 197/273 (72.16%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
F C C +SL G +YI+K+D C CYE+ +AN C++CS IGC+SKDLS+KDRHWHE+CFKC +CS SL D+ FA +++ L C++CY ++++C C K G RK EY+G WHE CFLC C+QP+GTKSFIP+E CVPC+E+++A +C C I GGVTY+ PWH+ECF C C +QL+G +FTS+E PYC EC+ L+AKKC CTKPIT G K+ISFE+R WHSECF C +C++SLVG+GFLT D I+C +CGR
Sbjct: 7 FDCHYCKDSLLGKKYIMKEDTQYCTKCYENLFANCCEECSTPIGCNSKDLSYKDRHWHEQCFKCAKCSRSLADKAFAAREDLLLCTECYALDYSSKCITCKKTILPGSRKMEYKGNSWHETCFLCHRCQQPLGTKSFIPKENGYFCVPCFEKQFAYQCCACKKAITTGGVTYQDKPWHRECFLCIGCKRQLSGQRFTSRENYPYCLECFSNLYAKKCVGCTKPITSLAGAKYISFEERQWHSECFNCMQCSVSLVGRGFLTQRDDILCTECGR 279 HSP 2 Score: 146.362 bits (368), Expect = 4.663e-39 Identity = 84/273 (30.77%), Postives = 121/273 (44.32%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAEC 1465
F C C + L+ Y + YC + Y L C C I + + H H F C C SL + ++D LC CY Y++ C C I S+ + +K WHE CF C C L + F K+N +C C++ +FA +C C K G Y+ K WH ECFLC+ C++ + + F RE C+ C+ YA+KC C I ++++ WH ECF C CS L G F ++ + C EC
Sbjct: 7 FDCHYCKDSLLGKKYIMKEDTQYCTKCYENLFANCCEECSTPIGCNSKDLSYKDRHWHEQCFKCAKCSRSLADKAFAAREDLLLCTECYALDYSSKCITCKKTILPGSRKMEYKGNSWHETCFLCHRCQQPLGTKSFIPKENGYFCVPCFEKQFAYQCCACKKAITTG--GVTYQDKPWHRECFLCIGCKRQLSGQRFTSRENYPYCLECFSNLYAKKCVGCTKPITSLAGAKYISFEERQWHSECFNCMQCSVSLVGRGFLTQRDDILCTEC 277 HSP 3 Score: 127.872 bits (320), Expect = 1.638e-32 Identity = 78/273 (28.57%), Postives = 125/273 (45.79%), Query Frame = 2
Query: 518 QDLGVGHVYRLDMAALPCTQCQSSIQAG-------QLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEK 1300
+D +G Y + CT+C ++ A + + + WH +CFKC +C L D + + C YA +C TC + I +R ME + + H F C C L +I K++ C+ C+E Q+A C C I + ++++D+ WH +CF C C L Q F +++N YC +C+ N +A +C GC K AG + + +QWH ECF C+ C + + F+ + DI+C C EK
Sbjct: 13 KDSLLGKKYIMKEDTQYCTKCYENLFANCCEECSTPIGCNSKDLSYKDRHWHEQCFKCAKCSRSLADKAFAAREDLLLCTECYALDYSSKCITCKKTILPG--SRKMEYKGNSWHETCFLCHRCQQPLGTKSFIPKENGYFCVPCFEKQFAYQCCACKKAI--TTGGVTYQDKPWHRECFLCIGCKRQLSGQRFTSRENYPYCLECFSNLYAKKCVGCTKPITSLAGAKYISFEERQWHSECFNCMQCSVSLVGRGFLTQRDDILCTECGREK 281
BLAST of Four and A half lim domains vs. Ensembl Medaka
Match: fhl3a (four and a half LIM domains 3 [Source:NCBI gene;Acc:101174533]) HSP 1 Score: 332.028 bits (850), Expect = 2.696e-109 Identity = 149/272 (54.78%), Postives = 194/272 (71.32%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCG 1657
F C +C SL G +YI +D P CI CY+S ++N C +C IG D+++L ++DRH+HE CF+CF C SL D+PF ++DN L C+DCY N+F+++C C K+ G RK EY G WHE CF+C C QPIG+KSFIP + + CVPCYE+K+A +CT+C + +GGVTY+ PWHKECF CT C QLAG FTS++E PYC +C+ L+AKKC C KPITGFGG K+ISFEDR WH CFTC +C++SLVG GF N D+I+C DC
Sbjct: 5 FDCDNCKESLYGRKYIQSNDSPYCIPCYDSLFSNTCDECKELIGHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDNALLCNDCYCNEFSSKCVACDKIVMPGTRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALSKGGVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPITGFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDCN 276 HSP 2 Score: 153.68 bits (387), Expect = 8.583e-42 Identity = 87/277 (31.41%), Postives = 128/277 (46.21%), Query Frame = 2
Query: 662 FKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH-FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGEL 1477
F C C E L Y ++ PYC+ Y L C C ELI + E H+ H F C CD SL + +D+ LC CY +++++ C C + ++ L + WHE CF C C + + F + YC CY++KFA RC C K G YR + WH+ECF+C C + + F R++ C+ C+ YA+KC C I G ++++ WH+ CFTC+ CS L G F ++ C +C L
Sbjct: 5 FDCDNCKESLYGRKYIQSNDSPYCIPCYDSLFSNTCDECKELIGHDARELFYEDRHYHEHCFRCFRCDRSLADEPFTSQDNALLCNDCYCNEFSSKCVACDKIVMPGTRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALSKG--GVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPITGFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDCNSNL 279 HSP 3 Score: 112.464 bits (280), Expect = 3.158e-27 Identity = 62/219 (28.31%), Postives = 100/219 (45.66%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAME---QEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
+H CF+C CD L D + N C Y +C CD+++ TR +E H F C SC+ + +I D+ C+ CYE ++A C +C + ++++D WH++CF C C + L Q F ++D + YC C+ + +A +C+ C K G + + +QWH+ CF C C + F P I+C C
Sbjct: 61 YHEHCFRCFRCDRSLADEPFTSQDNALLCNDCYCNEFSSKCVACDKIVMPG--TRKLEYAGSTWHEGCFICHSCEQPIGSKSFIPDKDEHYCVPCYEDKFAPRCTRCKKALS--KGGVTYRDEPWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPITGFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDC 275 HSP 4 Score: 53.9138 bits (128), Expect = 1.785e-7 Identity = 35/127 (27.56%), Postives = 55/127 (43.31%), Query Frame = 2
Query: 551 DMAALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIF---TEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGC 922
D A CT+C+ ++ G + R E WH CF CT C+ L + PYC++ ++ L +C C + I +Y +++ H + F C C SL G + D+ LC C
Sbjct: 156 DKFAPRCTRCKKALSKGGVTYRDE-------PWHKECFVCTSCETQLAGQHFTSRDESPYCLKCFSSLYAKKCEACCKPITGFGGGKYISFEDRQWHQSCFTCSQCSVSLVGAGFFPNGDRILCRDC 275
BLAST of Four and A half lim domains vs. Ensembl Medaka
Match: rragcb (Ras-related GTP binding Cb [Source:ZFIN;Acc:ZDB-GENE-070705-380]) HSP 1 Score: 300.056 bits (767), Expect = 1.546e-91 Identity = 131/272 (48.16%), Postives = 185/272 (68.01%), Query Frame = 2
Query: 842 FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCG 1657
F C++C SL G +YI +++P CI CY+ +AN CQ+C+ IG + K+L+FKDR++HE CF+CF C SL + F + ++ L C+DCY N+F+++C C+K+ G + E+ G WHE+CF+C C +PIG +SF+P E CVPCYE ++ +C+ C + +GGV+Y+G WH+ECF CT CS LAG FTS+ E PYC +C+ L+AKKC+ C ITGFG K+ISFEDR WH CF C +C++SLVG GF D+I+C DC
Sbjct: 449 FDCRNCKASLYGQQYIKVENEPHCINCYDQLHANTCQECTEIIGHNDKELTFKDRYYHEHCFRCFRCDRSLSNASFTSHEDVLLCNDCYCNEFSSKCVSCNKIVMPGSKILEFDGSSWHEDCFVCHSCEKPIGGESFVPNENQYYCVPCYEGRFTPRCSHCNKALTKGGVSYQGGVWHRECFLCTGCSAPLAGQPFTSQGETPYCVKCFSNLYAKKCAGCNSAITGFGDGKYISFEDREWHQPCFKCSRCSVSLVGSGFFPYRDQILCRDCN 720 HSP 2 Score: 157.147 bits (396), Expect = 6.412e-40 Identity = 88/282 (31.21%), Postives = 128/282 (45.39%), Query Frame = 2
Query: 635 TQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH-FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAEC 1465
T S F C C L Y N+P+C+ Y QL C C E+I + + ++ H F C CD SL+ + +D LC CY +++++ C C+ + SK L F WHE CF C C + + F +N YC CY+ +F RC C+K G Y+G WH ECFLC C P+ + F + + CV C+ YA+KC C I G ++++ WH+ CF C+ CS L G F ++ C +C
Sbjct: 440 TDASIMSERFDCRNCKASLYGQQYIKVENEPHCINCYDQLHANTCQECTEIIGHNDKELTFKDRYYHEHCFRCFRCDRSLSNASFTSHEDVLLCNDCYCNEFSSKCVSCNKIVMPGSKILEFDGSSWHEDCFVCHSCEKPIGGESFVPNENQYYCVPCYEGRFTPRCSHCNKALTKG--GVSYQGGVWHRECFLCTGCSAPLAGQPFTSQGETPYCVKCFSNLYAKKCAGCNSAITGFGDGKYISFEDREWHQPCFKCSRCSVSLVGSGFFPYRDQILCRDC 719 HSP 3 Score: 108.997 bits (271), Expect = 3.028e-24 Identity = 59/222 (26.58%), Postives = 105/222 (47.30%), Query Frame = 2
Query: 647 WHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAH---FACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKA-GMRKY-EYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEE 1297
+H CF+C CD L + + + + C Y +C +C++++ ++ +E + H F C SC+ + G ++ ++Q C+ CYE ++ C C+ + +S++ WH +CF C CS+ L QPF ++ YC C+ N +A +C GC+ G KY + ++WH+ CF C C + F P I+C C ++
Sbjct: 505 YHEHCFRCFRCDRSLSNASFTSHEDVLLCNDCYCNEFSSKCVSCNKIVMPG--SKILEFDGSSWHEDCFVCHSCEKPIGGESFVPNENQYYCVPCYEGRFTPRCSHCNKALT--KGGVSYQGGVWHRECFLCTGCSAPLAGQPFTSQGETPYCVKCFSNLYAKKCAGCNSAITGFGDGKYISFEDREWHQPCFKCSRCSVSLVGSGFFPYRDQILCRDCNDD 722 HSP 4 Score: 93.9745 bits (232), Expect = 1.607e-19 Identity = 47/153 (30.72%), Postives = 79/153 (51.63%), Query Frame = 2
Query: 1202 ECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG--VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDC 1654
E F C +C+ + + +I E + C+ CY++ +A C +C +I +T+K +H+ CF C C + L+ FTS E+ C +CY F+ KC C K + G K + F+ WH +CF C+ C + G+ F+ N+++ C C
Sbjct: 447 ERFDCRNCKASLYGQQYIKVENEPHCINCYDQLHANTCQECTEIIGHNDKELTFKDRYYHEHCFRCFRCDRSLSNASFTSHEDVLLCNDCYCNEFSSKCVSCNKIV--MPGSKILEFDGSSWHEDCFVCHSCEKPIGGESFVPNENQYYCVPC 597 HSP 5 Score: 91.2781 bits (225), Expect = 9.580e-19 Identity = 56/184 (30.43%), Postives = 83/184 (45.11%), Query Frame = 2
Query: 569 CTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGC--DSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDN 1114
C C + G + + + SWH CF C C++ + + N+ YCV Y PRC+ C++ + T+ H F C C L G + + + P C+ C+ + YA C C+ I D K +SF+DR WH+ CFKC CS SLV F + + C DC D+
Sbjct: 545 CVSCNKIVMPGSKILEFDGS-----SWHEDCFVCHSCEKPIGGESFVPNENQYYCVPCYEGRFTPRCSHCNKAL-TKGGVSYQGGVWHRECFLCTGCSAPLAGQPFTSQGETPYCVKCFSNLYAKKCAGCNSAITGFGDGKYISFEDREWHQPCFKCSRCSVSLVGSGFFPYRDQILCRDCNDD 722
BLAST of Four and A half lim domains vs. Planmine SMEST
Match: SMESG000072127.1 (SMESG000072127.1) HSP 1 Score: 1097.03 bits (2836), Expect = 0.000e+0 Identity = 529/529 (100.00%), Postives = 529/529 (100.00%), Query Frame = 2
Query: 74 MALKQDIGTPCLDCVDKCRGFKIHGWRKTCLGCDCSVDRHDITDESPLPILEEMKLADKPYYNLFMESLKMARKYGLSWLPIGLQASEVEQFTSIVGGNIASRAGEKLRKQKLRRQIPVHDRCVEKFSHLETPTERQEAEKFLQFRNNQDLGVGHVYRLDMAALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
MALKQDIGTPCLDCVDKCRGFKIHGWRKTCLGCDCSVDRHDITDESPLPILEEMKLADKPYYNLFMESLKMARKYGLSWLPIGLQASEVEQFTSIVGGNIASRAGEKLRKQKLRRQIPVHDRCVEKFSHLETPTERQEAEKFLQFRNNQDLGVGHVYRLDMAALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR
Sbjct: 1 MALKQDIGTPCLDCVDKCRGFKIHGWRKTCLGCDCSVDRHDITDESPLPILEEMKLADKPYYNLFMESLKMARKYGLSWLPIGLQASEVEQFTSIVGGNIASRAGEKLRKQKLRRQIPVHDRCVEKFSHLETPTERQEAEKFLQFRNNQDLGVGHVYRLDMAALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 529
BLAST of Four and A half lim domains vs. Planmine SMEST
Match: SMESG000037769.1 (SMESG000037769.1) HSP 1 Score: 788.875 bits (2036), Expect = 0.000e+0 Identity = 363/533 (68.11%), Postives = 444/533 (83.30%), Query Frame = 2
Query: 74 MALKQDIGTPCLDCVDKCRGFKIHGWRKTCLGCDCSVDRHDITDESPLPILEEMKLADKPYYNLFMESLKMARKYGLSWLPIGLQASEVEQFTSIVGGNIASRA---GEKLRKQKLRRQIPVHDRCVEKFSHLETPTERQEAEKFLQFRNNQDLGVGHVYRL-DMAALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
MALKQDIG+PC++C D+C+GF++HGWRKTC C+CS D HD+ D++ +L+EMKL+DK +Y ++E+ K + GL+WLPIGL+ EVE F S + + +K R ++LR+QIPV DR ++KFSHL+T ERQEAEKFL+ RN+ DL + HV + + + C +C+++I +L+VR++H + SWHP CF C+EC E+LVDYIYCW+ NK YC RHY + IRPRCATCDELIF EEYTRAM+QEHHP HFACK+CDNSLTGHRYILK++ P CI CYE+++AN C+QC +IGCDSKDLSFKDRHWHEKCF+CFEC +SLVD+PFATKD +L+CS+CYDNKFA RCDGC KVFKAGMRKYEYRGKQWHEECFLC++C+QPIG KSFIPR+Q I+CVPCYE KYAQKC+KC VIRRGGVTYKG+PWHKECFTCT+C+KQLAGIKFTSK++KPYCA+CYGELFAKKC+KCTKPITGFGGCKFISFE RHWHSECFTC KC +LVG+GFLTN+D I+CPDCGR
Sbjct: 1 MALKQDIGSPCIECNDRCKGFQLHGWRKTCTACNCSYDIHDVFDDNISCVLDEMKLSDKSFYQKYIEAQKQGKASGLNWLPIGLKPIEVESFLSSLPASANPHGVGDDDKSRLRRLRKQIPVQDRRLDKFSHLDTNVERQEAEKFLKNRNSHDLSIAHVEAITEFQGVQCFECKTTIGKSELSVRIDHNESGAHSWHPACFNCSECKELLVDYIYCWHLNKLYCARHYGEKIRPRCATCDELIFNEEYTRAMDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 533
BLAST of Four and A half lim domains vs. Planmine SMEST
Match: SMESG000037769.1 (SMESG000037769.1) HSP 1 Score: 498.819 bits (1283), Expect = 9.333e-175 Identity = 229/282 (81.21%), Postives = 261/282 (92.55%), Query Frame = 2
Query: 815 MEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCGR 1660
M+QEHHP HFACK+CDNSLTGHRYILK++ P CI CYE+++AN C+QC +IGCDSKDLSFKDRHWHEKCF+CFEC +SLVD+PFATKD +L+CS+CYDNKFA RCDGC KVFKAGMRKYEYRGKQWHEECFLC++C+QPIG KSFIPR+Q I+CVPCYE KYAQKC+KC VIRRGGVTYKG+PWHKECFTCT+C+KQLAGIKFTSK++KPYCA+CYGELFAKKC+KCTKPITGFGGCKFISFE RHWHSECFTC KC +LVG+GFLTN+D I+CPDCGR
Sbjct: 1 MDQEHHPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDCGR 282 HSP 2 Score: 166.007 bits (419), Expect = 2.214e-46 Identity = 87/277 (31.41%), Postives = 133/277 (48.01%), Query Frame = 2
Query: 650 HPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEH-HPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGG----VTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAEC 1465
HP F C CD L + Y P+C++ Y C C E I + + + H H F C C SL + KD+ C CY++++A C C+ + ++ + WHE+CF C EC + + F +D + C CY+NK+A +C C +V + G Y+G WH+ECF C +C + + F ++ C CY E +A+KCTKC I G ++++G WH ECFTC+ C+ L G F + E+ C +C
Sbjct: 6 HPNHFACKNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEECFLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVIRRG--GVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDC 280 HSP 3 Score: 123.25 bits (308), Expect = 5.317e-31 Identity = 76/271 (28.04%), Postives = 119/271 (43.91%), Query Frame = 2
Query: 509 RNNQDLGVGHVYRLDMAALPCTQCQSSIQAG-------QLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELI--FTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFK--AGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPC 1288
+N + GH Y L C +C + A ++ + + WH +CF+C EC LVD + +C Y RC C ++ +Y +Q H F C C + +I +D Q +C+ CYE++YA C +C+ I +++K WH++CF C C+ L F +KD+ YC+DCY FA +C C K G + + G+ WH ECF C C + + F+ E +I+C C
Sbjct: 13 KNCDNSLTGHRYILKEENPHCIKCYETKFANTCEQCKEKIGCDSKDLSFKDRHWHEKCFECFECHTSLVDRPFATKDESLFCSECYDNKFAARCDGCTKVFKAGMRKYEYRGKQWHEEC-FLCLECKQPIGAKSFIPRDQQIICVPCYENKYAQKCSKCTEVI--RRGGVTYKGTPWHKECFTCTNCTKQLAGIKFTSKDDKPYCADCYGELFAKKCTKCTKPITGFGGCKFISFEGRHWHSECFTCSKCTTNLVGRGFLTNEDNIMCPDC 280
BLAST of Four and A half lim domains vs. Planmine SMEST
Match: SMESG000004969.1 (SMESG000004969.1) HSP 1 Score: 504.597 bits (1298), Expect = 3.354e-173 Identity = 252/531 (47.46%), Postives = 339/531 (63.84%), Query Frame = 2
Query: 74 MALKQDIGTPCLDCVDKCRGFKIHGWRKTCLGCDCSVDRHDITDESPLPILEEMKLADKPYYNLFMESLKMARKYGLSWLPIGLQASE-VEQFTSIVGGNIASRAGEKLRKQK--LRRQIPVHDRCVEKFSHLETPTERQEAEKFLQFRNNQDLGVGHVYRLDMAALPCTQCQSSIQAGQLAVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKPYCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCG 1657
MALKQDIG C +C + C GFKIHGWRKTC C C+VD+HDI DES LPIL+EMK+ DKP + E+L +A K G WLP+GL+ +E+F I ++A L K+K LR QIPV DRCVEKF+HL T ER+EAEKFL RNN+D+G+G V R+ + CT+C ++I+ L V E + + WH CFKCT C E+LVDYIY Y+ K YC+RHYA+ IRPRC+ CDELIF+ EY RA + E H HFAC CD SL+ Y+ CI CY + +N C +CS +I CDSKDLS+ + HWH CF C C+ +L F + LYC +C+ +K +C C + + G + + K WH CF C DC++PIG SF+ +++ +C C++ KYA++C KC +++ GG++YK +H +CF C +C ++A KF K + YC C LF+ +C KCTKPITG CK+ISFED++WH CF C KC++SLV KGF D+ +CP+C
Sbjct: 1 MALKQDIGLSCNNCSN-CTGFKIHGWRKTCDKCKCAVDKHDIFDESTLPILDEMKV-DKPISAEYKEALAVAGKVGKPWLPVGLKNKNMIEEF-------ILNKAYPNLLKRKKCLRLQIPVQDRCVEKFNHLGTLAEREEAEKFLLNRNNRDIGLG-VVRISDEHMNCTECHTNIKPNTLCVTNEQTSAVKQFWHLECFKCTSCQELLVDYIYASYNQKLYCIRHYAKNIRPRCSECDELIFSVEYIRAGDNEFHLNHFACYICDASLSKQSYLQHKGFATCIQCYNHELSNKCVKCSKQISCDSKDLSYNNNHWHSDCFMCSICNKNLEGNQFLYLNELLYCVNCHQDKILPKCISCDRPIENGAKMICFDEKNWHFNCFNCKDCKRPIGNDSFVQQDKRSICSKCFDAKYAKRCAKCEKLVKEGGISYKNQSYHYDCFNCANCKCRIADTKFLVKNNENYCKVCCDRLFSTRCKKCTKPITGDAICKYISFEDKNWHVGCFNCDKCHVSLVDKGFFNKDNDTLCPNCS 521
BLAST of Four and A half lim domains vs. Planmine SMEST
Match: SMESG000009923.1 (SMESG000009923.1) HSP 1 Score: 487.649 bits (1254), Expect = 3.329e-166 Identity = 248/537 (46.18%), Postives = 332/537 (61.82%), Query Frame = 2
Query: 125 CRGFKIHGWRKTCLGCDCSVDRHDITDESPLPI------------LEEMKLADKPYYNLFMESLKMARKYGLSWLPIGLQASEVEQFTSIVGGNIASRAGEKL------RKQKLRRQIPVHDRCVEKF-SHLETPTER----QEAEKFLQFRNNQDLGVGHVYRLDMAALPCTQCQSSIQAGQL-AVRVEHAANTQMSWHPRCFKCTECDEVLVDYIYCWYSNKP--YCVRHYAQLIRPRCATCDELIFTEEYTRAMEQEHHPAHFACKSCDNSLTGHRYILKDDQPLCIGCYESQYANICQQCSGRIGCDSKDLSFKDRHWHEKCFKCFECSSSLVDQPFATKDNNLYCSDCYDNKFATRCDGCHKVFKAGMRKYEYRGKQWHEECFLCVDCRQPIGTKSFIPREQDIVCVPCYEEKYAQKCTKCVGVIRRGGVTYKGSPWHKECFTCTHCSKQLAGIKFTSKEEKPYCAECYGELFAKKCSKCTKPITGFGGCKFISFEDRHWHSECFTCYKCNISLVGKGFLTNDDKIICPDCG 1657
C + H WR C+ C C RH ++ + L ++ + + + ++ MA L WLPIGLQ +E+ F + + E + RK + QIP DR + +H + E Q A F + R+ LG G + +D + C+ C + + +R+ H+A + + H CF+C C E LVD+ YCW ++ +C+RH+ +L RCA CDELI +++T+AM+QE H HFAC CD+SLTG +YIL+DD P C+ C+E+Q+ N C +CS +I CDSKDLSFK+ HWHEKCF C C SLVD+ F TKDN LYC +C+D KF++ CDGC K+F+ G++KYEY GKQWHE+CF+C DC Q IGTKSFIP I C+ CYE K+AQKC KC +IR+ G+TYKG PWHKECF CT C K L+ KFTSKEEKPYC EC+ ELF K+C KCT+PI+GFGGCKFIS D HWH+ECF C KC S+VGKGF +D+++C CG
Sbjct: 9 CDKYTNHKWRFRCVSCRCPNYRH-VSHVNSLDSGSSTGQHCVNNWIDSLTMESHNQLQDYKAAISMAESNNLVWLPIGLQQAEISDFLRVWNDFGPGFSLENIPINQLDRKMNIFYQIPDCDRISSEVQNHFDDKDEDKLIVQLARAFKKSRDTNYLGFGEIEPVDNQEV-CSICDKQFEDEDIFCIRITHSA--EFASHISCFECKTCKEKLVDFHYCWSNDSKAIFCLRHFGELTYCRCAECDELIVNKDFTKAMDQEFHVEHFACHVCDSSLTGTKYILRDDNPFCVKCFENQFGNTCHKCSEKISCDSKDLSFKEWHWHEKCFLCDICHDSLVDRSFTTKDNYLYCPNCFDEKFSSCCDGCGKIFRTGVKKYEYAGKQWHEDCFICADCNQSIGTKSFIPINGKISCIGCYENKHAQKCFKCSEIIRKSGITYKGDPWHKECFLCTSCQKMLSESKFTSKEEKPYCTECFIELFCKRCKKCTQPISGFGGCKFISVGDDHWHTECFNCSKCQASMVGKGFFLQEDQMVCGSCG 541 The following BLAST results are available for this feature:
BLAST of Four and A half lim domains vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Four and A half lim domains vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Four and A half lim domains vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of Four and A half lim domains vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Four and A half lim domains vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Four and A half lim domains vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30024345 ID=SMED30024345|Name=Four and A half lim domains|organism=Schmidtea mediterranea sexual|type=transcript|length=1980bpback to top protein sequence of SMED30024345-orf-1 >SMED30024345-orf-1 ID=SMED30024345-orf-1|Name=SMED30024345-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=530bp MALKQDIGTPCLDCVDKCRGFKIHGWRKTCLGCDCSVDRHDITDESPLPIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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