SMED30024269

Overview
NameSMED30024269
Smed IDSMED30024269
Length (bp)1928
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30024269 (SMED30024269) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30024269 (SMED30024269) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30024269 (SMED30024269) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30024269 (SMED30024269) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30024269

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30024269SMESG000058883.1 SmedASXL_017351SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30024269SMESG000058883.1 SmedASXL_017351SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
head regionSMED30024269SMESG000058883.1 dd_Smed_v6_15587_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30024269 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001145:29109..31253 +9612
Homology
[Close]
BLAST of SMED30024269 vs. Planmine SMEST
Match: SMESG000058883.1 (SMESG000058883.1)

HSP 1 Score: 1233.78 bits (3191), Expect = 0.000e+0
Identity = 613/613 (100.00%), Postives = 613/613 (100.00%), Query Frame = 3 
Query:   18 MIHFIICLIYLLFCNLVENATALVWYMIYLINLFIFYICCNWVSLVPIWNPDIRLTNGVCNEVTLTAKCPTLSTRRSIEFELYPLYDPTKSVIQTLQCRINQSQPLTTTFSIQCKNLYRVNISLSPIFSCTEYCSDIHTSRIWLLGGNTCGEIALDCAFNVHLPRENSCINSSIQCSLDSTVQSATRVSPMSLNPRLVNAYCKNVTVEASCPQSPEDQYIEFILSLSNQKHLESISKLCQSNATKLVQVTFKISCKNFYYVEVXXXXXXXKNSKVPLRNQRSATWILGDVICSNDIKEKCRFIDQFKVPENKCYTSTVNCTDSSQMSLTTPSSQWSPNIKIVKAFCDNVTVSVDCPRVNKQREIEINLIKDFRNEEFSTRICKLNSSETLEISFNLKCMNIYHFNVSMKPTFSPFECDLYCSNPLSSQKYLLGSRLCRNSSFDDCKYDAYKTKNNLCPKSSKKCPEIINGSSSHKIWESQLKIVQDFCSNVTVKASCPDLKDRINFLLFEKGNSQPIKGRSVPCNSSRGYTVAAHFRIRCRNLYQFIANLIRNTNKTEPYKKKNESILSQFWVFGTENCPENKFNICQSAKYRFNSTKCADGYYRCSKPVDGK 1856
            MIHFIICLIYLLFCNLVENATALVWYMIYLINLFIFYICCNWVSLVPIWNPDIRLTNGVCNEVTLTAKCPTLSTRRSIEFELYPLYDPTKSVIQTLQCRINQSQPLTTTFSIQCKNLYRVNISLSPIFSCTEYCSDIHTSRIWLLGGNTCGEIALDCAFNVHLPRENSCINSSIQCSLDSTVQSATRVSPMSLNPRLVNAYCKNVTVEASCPQSPEDQYIEFILSLSNQKHLESISKLCQSNATKLVQVTFKISCKNFYYVEVSLLSSSLKNSKVPLRNQRSATWILGDVICSNDIKEKCRFIDQFKVPENKCYTSTVNCTDSSQMSLTTPSSQWSPNIKIVKAFCDNVTVSVDCPRVNKQREIEINLIKDFRNEEFSTRICKLNSSETLEISFNLKCMNIYHFNVSMKPTFSPFECDLYCSNPLSSQKYLLGSRLCRNSSFDDCKYDAYKTKNNLCPKSSKKCPEIINGSSSHKIWESQLKIVQDFCSNVTVKASCPDLKDRINFLLFEKGNSQPIKGRSVPCNSSRGYTVAAHFRIRCRNLYQFIANLIRNTNKTEPYKKKNESILSQFWVFGTENCPENKFNICQSAKYRFNSTKCADGYYRCSKPVDGK
Sbjct:    1 MIHFIICLIYLLFCNLVENATALVWYMIYLINLFIFYICCNWVSLVPIWNPDIRLTNGVCNEVTLTAKCPTLSTRRSIEFELYPLYDPTKSVIQTLQCRINQSQPLTTTFSIQCKNLYRVNISLSPIFSCTEYCSDIHTSRIWLLGGNTCGEIALDCAFNVHLPRENSCINSSIQCSLDSTVQSATRVSPMSLNPRLVNAYCKNVTVEASCPQSPEDQYIEFILSLSNQKHLESISKLCQSNATKLVQVTFKISCKNFYYVEVSLLSSSLKNSKVPLRNQRSATWILGDVICSNDIKEKCRFIDQFKVPENKCYTSTVNCTDSSQMSLTTPSSQWSPNIKIVKAFCDNVTVSVDCPRVNKQREIEINLIKDFRNEEFSTRICKLNSSETLEISFNLKCMNIYHFNVSMKPTFSPFECDLYCSNPLSSQKYLLGSRLCRNSSFDDCKYDAYKTKNNLCPKSSKKCPEIINGSSSHKIWESQLKIVQDFCSNVTVKASCPDLKDRINFLLFEKGNSQPIKGRSVPCNSSRGYTVAAHFRIRCRNLYQFIANLIRNTNKTEPYKKKNESILSQFWVFGTENCPENKFNICQSAKYRFNSTKCADGYYRCSKPVDGK 613
[Close]
BLAST of SMED30024269 vs. Planmine SMEST
Match: SMESG000058905.1 (SMESG000058905.1)

HSP 1 Score: 211.075 bits (536), Expect = 1.076e-60
Identity = 141/449 (31.40%), Postives = 225/449 (50.11%), Query Frame = 3 
Query:  153 VPIWNPDIRLTNGVCNEVTLTAKCPTLSTRRSIEFELYPLYDPTKSV--IQTLQCRINQSQPLTTTFSIQCKNLYRVNISLSPIF---SCTEYCSDIHTSRIWLLGGNTCGE---IALDCAFNVHLPRENSCINSSIQCSLDSTVQS----ATRVSPMSLNPRLVNAYCKNVTVEASCPQSPEDQYIEFILSLSN--QKHLESISKLCQSNATKLVQVTFKISCKNFYYVEVXXXXXXXKNSKVPLRNQRS-ATWILGDVICSNDIKEKCRFIDQFKVPENKCYTSTVNCTDSSQMSLTTPSSQWSPNIKIVKAFCDNVTVSVDCPRVNKQREIEINLIK--DFRNEEFSTRICKLNSSETLEISFNLKCMNIYHFNVSMKPTFSPFECDLYCSNPLSSQKYLLGSRLCRNSSFDDCKYDAYKTKNNLCPKSSKKCPEIINGSSSHKIW 1448
            + IW+P++ L +  C+ V + A CP L   R+IEF L+    PT++V  ++T +C  + ++ L  +  + C NLY+VNISLSPIF   +C EYC + H+S IWL  G+ C     ++ + + N ++  +N C  S+ +CS D   +S       +       +L +A C+N   +  CP       + FI    N  Q H E I K C+ N + ++ V+F +SCK+ Y + +S   ++          +     W+LG   C  + +  C++ +Q+K   NKC  S+  C +          +QWSPNIK++ A+C N+TV   CP +N  R +E +L++  D       T+IC   S   L++SF  +C  +Y+ N+ M P      CD +CS   SS+ +LLG +LC     D CK   Y+   N CP     C       S  K+W
Sbjct:   18 IKIWDPNLTLISANCSNVNIEATCPKLVKERNIEFSLFEF--PTQNVQSVKTYKCPASNAKKLHVSMKLPCINLYQVNISLSPIFPMINCREYCLEAHSSPIWLF-GDICQNKQFLSNNGSLNKYMTPKNICPASTQKCSADLKTESQKSFMQTIKKTLSKAKLTDADCENAIFQMECPLFNFGSQLTFIFYRQNHFQNHSE-IMKHCKPNNSNVINVSFSLSCKSLYLLHISFKPTNESICDGSYSVEHPIQMWLLGGQHCFEEFENDCQY-EQYKSNRNKCDISSRRCREMQSF------TQWSPNIKLITAYCTNITVQATCPFLNVSRNLEFSLLRMGDRSISATVTKICNPLSKTYLKVSFEFECRAVYYVNIIMSPITPSMICDEFCSKQHSSEMWLLGRKLCPKVGLDYCKVRKYEINENHCPIHLDTCKSF---QSYKKVW 452

HSP 2 Score: 150.214 bits (378), Expect = 1.011e-38
Identity = 113/419 (26.97%), Postives = 195/419 (46.54%), Query Frame = 3 
Query:  597 NPRLVNAYCKNVTVEASCPQSPEDQYIEFILSLSNQKHLESISKL-CQSNATKLVQVTFKISCKNFYYVEVXXXXX--XXKNSKVPLRNQRSATWILGDVICSNDIKEKCRFIDQFKVPENKCYTSTVNCT----DSSQMSLTTPSSQWSPNIKIVKAFCDNVTVSVDCPRVNKQREIEINLIKD--FRNEEFSTRICKLNSSETLEISFNLKCMNIYHFNVSMKPTFSPFECDLYCSNPLSSQKYLLGSRLCRNSSFDDCKYDAYKTKNNLCPKSSKKCPEIINGSSSHKIWESQLKIVQDFCSNVTVKASCP--DLKDRINFLLFEKGNSQPIKGRSVPCNSSRGYTVAAHFRIRCRNLY--QFIANLIRNTNKTEPYKKKNESILSQFWVFGTENCPENKFNICQSAKYRFNSTKC 1814
            N  L++A C NV +EA+CP+  +++ IEF L     ++++S+    C ++  K + V+ K+ C N Y V +SL      +   +  L    S  W+ GD+  +         ++++  P+N C  ST  C+      SQ S      +     K+  A C+N    ++CP  N   ++     +   F+N     + CK N+S  + +SF+L C ++Y  ++S KPT     CD   S     Q +LLG + C     +DC+Y+ YK+  N C  SS++C E+     S   W   +K++  +C+N+TV+A+CP  ++   + F L   G+       +  CN      +   F   CR +Y    I + I  +   + +  K  S  S+ W+ G + CP+   + C+  KY  N   C
Sbjct:   24 NLTLISANCSNVNIEATCPKLVKERNIEFSLFEFPTQNVQSVKTYKCPASNAKKLHVSMKLPCINLYQVNISLSPIFPMINCREYCLEAHSSPIWLFGDICQNKQFLSNNGSLNKYMTPKNICPASTQKCSADLKTESQKSFMQTIKKTLSKAKLTDADCENAIFQMECPLFNFGSQLTFIFYRQNHFQNHSEIMKHCKPNNSNVINVSFSLSCKSLYLLHISFKPTNESI-CDGSYSVEHPIQMWLLGGQHCFEEFENDCQYEQYKSNRNKCDISSRRCREM----QSFTQWSPNIKLITAYCTNITVQATCPFLNVSRNLEFSLLRMGDRSISATVTKICNPLSKTYLKVSFEFECRAVYYVNIIMSPITPSMICDEFCSKQHS--SEMWLLGRKLCPKVGLDYCKVRKYEINENHC 435

HSP 3 Score: 113.235 bits (282), Expect = 2.957e-26
Identity = 78/294 (26.53%), Postives = 130/294 (44.22%), Query Frame = 3 
Query: 1020 WSPNIKIVKAFCDNVTVSVDCPRVNKQREIEINLIKDFRNE---EFSTRICKLNSSETLEISFNLKCMNIYHFNVSMKPTFSPFECDLYCSNPLSSQKYLLGSRLCRNSSF--DDCKYDAYKTKNNLCPKSSKKCPEIINGSSSHKIWE------SQLKIVQDFCSNVTVKASCP--DLKDRINFLLFEKGNSQPIKGRSVPCNSSRGYTVAAHFRIRCRNLYQFIANLIRNTNKTEPYKKKNESILS---------QFWVFGTENCPENKFNICQSAKYRFNSTKCADGYYRC 1835
            W PN+ ++ A C NV +   CP++ K+R IE +L  +F  +      T  C  ++++ L +S  L C+N+Y  N+S+ P F    C  YC    SS  +L G  +C+N  F  ++   + Y T  N+CP S++KC   +   S     +      S+ K+    C N   +  CP  +   ++ F+ + + + Q        C  +    +   F + C++LY    +          +K  NESI           Q W+ G ++C E   N CQ  +Y+ N  KC     RC
Sbjct:   21 WDPNLTLISANCSNVNIEATCPKLVKERNIEFSLF-EFPTQNVQSVKTYKCPASNAKKLHVSMKLPCINLYQVNISLSPIFPMINCREYCLEAHSSPIWLFGD-ICQNKQFLSNNGSLNKYMTPKNICPASTQKCSADLKTESQKSFMQTIKKTLSKAKLTDADCENAIFQMECPLFNFGSQLTFIFYRQNHFQNHSEIMKHCKPNNSNVINVSFSLSCKSLYLLHIS----------FKPTNESICDGSYSVEHPIQMWLLGGQHCFEEFENDCQYEQYKSNRNKCDISSRRC 302
[Close]
BLAST of SMED30024269 vs. Planmine SMEST
Match: SMESG000058905.1 (SMESG000058905.1)

HSP 1 Score: 210.69 bits (535), Expect = 1.613e-60
Identity = 141/449 (31.40%), Postives = 225/449 (50.11%), Query Frame = 3 
Query:  153 VPIWNPDIRLTNGVCNEVTLTAKCPTLSTRRSIEFELYPLYDPTKSV--IQTLQCRINQSQPLTTTFSIQCKNLYRVNISLSPIF---SCTEYCSDIHTSRIWLLGGNTCGE---IALDCAFNVHLPRENSCINSSIQCSLDSTVQS----ATRVSPMSLNPRLVNAYCKNVTVEASCPQSPEDQYIEFILSLSN--QKHLESISKLCQSNATKLVQVTFKISCKNFYYVEVXXXXXXXKNSKVPLRNQRS-ATWILGDVICSNDIKEKCRFIDQFKVPENKCYTSTVNCTDSSQMSLTTPSSQWSPNIKIVKAFCDNVTVSVDCPRVNKQREIEINLIK--DFRNEEFSTRICKLNSSETLEISFNLKCMNIYHFNVSMKPTFSPFECDLYCSNPLSSQKYLLGSRLCRNSSFDDCKYDAYKTKNNLCPKSSKKCPEIINGSSSHKIW 1448
            + IW+P++ L +  C+ V + A CP L   R+IEF L+    PT++V  ++T +C  + ++ L  +  + C NLY+VNISLSPIF   +C EYC + H+S IWL  G+ C     ++ + + N ++  +N C  S+ +CS D   +S       +       +L +A C+N   +  CP       + FI    N  Q H E I K C+ N + ++ V+F +SCK+ Y + +S   ++          +     W+LG   C  + +  C++ +Q+K   NKC  S+  C +          +QWSPNIK++ A+C N+TV   CP +N  R +E +L++  D       T+IC   S   L++SF  +C  +Y+ N+ M P      CD +CS   SS+ +LLG +LC     D CK   Y+   N CP     C       S  K+W
Sbjct:   21 IQIWDPNLTLISANCSNVNIEATCPKLVKERNIEFSLFEF--PTQNVQSVKTYKCPASNAKKLHVSMKLPCINLYQVNISLSPIFPMINCREYCLEAHSSPIWLF-GDICQNKQFLSNNGSLNKYMTPKNICPASTQKCSADLKTESQKSFMQTIKKTLSKAKLTDADCENAIFQMECPLFNFGSQLTFIFYRQNHFQNHSE-IMKHCKPNNSNVINVSFSLSCKSLYLLHISFKPTNESICDGSYSVEHPIQMWLLGGQHCFEEFENDCQY-EQYKSNRNKCDISSRRCREMQSF------TQWSPNIKLITAYCTNITVQATCPFLNVSRNLEFSLLRMGDRSISATVTKICNPLSKTYLKVSFEFECRAVYYVNIIMSPITPSMICDEFCSKQHSSEMWLLGRKLCPKVGLDYCKVRKYEINENHCPIHLDTCKSF---QSYKKVW 455

HSP 2 Score: 150.214 bits (378), Expect = 9.591e-39
Identity = 113/419 (26.97%), Postives = 195/419 (46.54%), Query Frame = 3 
Query:  597 NPRLVNAYCKNVTVEASCPQSPEDQYIEFILSLSNQKHLESISKL-CQSNATKLVQVTFKISCKNFYYVEVXXXXX--XXKNSKVPLRNQRSATWILGDVICSNDIKEKCRFIDQFKVPENKCYTSTVNCT----DSSQMSLTTPSSQWSPNIKIVKAFCDNVTVSVDCPRVNKQREIEINLIKD--FRNEEFSTRICKLNSSETLEISFNLKCMNIYHFNVSMKPTFSPFECDLYCSNPLSSQKYLLGSRLCRNSSFDDCKYDAYKTKNNLCPKSSKKCPEIINGSSSHKIWESQLKIVQDFCSNVTVKASCP--DLKDRINFLLFEKGNSQPIKGRSVPCNSSRGYTVAAHFRIRCRNLY--QFIANLIRNTNKTEPYKKKNESILSQFWVFGTENCPENKFNICQSAKYRFNSTKC 1814
            N  L++A C NV +EA+CP+  +++ IEF L     ++++S+    C ++  K + V+ K+ C N Y V +SL      +   +  L    S  W+ GD+  +         ++++  P+N C  ST  C+      SQ S      +     K+  A C+N    ++CP  N   ++     +   F+N     + CK N+S  + +SF+L C ++Y  ++S KPT     CD   S     Q +LLG + C     +DC+Y+ YK+  N C  SS++C E+     S   W   +K++  +C+N+TV+A+CP  ++   + F L   G+       +  CN      +   F   CR +Y    I + I  +   + +  K  S  S+ W+ G + CP+   + C+  KY  N   C
Sbjct:   27 NLTLISANCSNVNIEATCPKLVKERNIEFSLFEFPTQNVQSVKTYKCPASNAKKLHVSMKLPCINLYQVNISLSPIFPMINCREYCLEAHSSPIWLFGDICQNKQFLSNNGSLNKYMTPKNICPASTQKCSADLKTESQKSFMQTIKKTLSKAKLTDADCENAIFQMECPLFNFGSQLTFIFYRQNHFQNHSEIMKHCKPNNSNVINVSFSLSCKSLYLLHISFKPTNESI-CDGSYSVEHPIQMWLLGGQHCFEEFENDCQYEQYKSNRNKCDISSRRCREM----QSFTQWSPNIKLITAYCTNITVQATCPFLNVSRNLEFSLLRMGDRSISATVTKICNPLSKTYLKVSFEFECRAVYYVNIIMSPITPSMICDEFCSKQHS--SEMWLLGRKLCPKVGLDYCKVRKYEINENHC 438

HSP 3 Score: 113.62 bits (283), Expect = 2.596e-26
Identity = 77/293 (26.28%), Postives = 129/293 (44.03%), Query Frame = 3 
Query: 1020 WSPNIKIVKAFCDNVTVSVDCPRVNKQREIEINLIK--DFRNEEFSTRICKLNSSETLEISFNLKCMNIYHFNVSMKPTFSPFECDLYCSNPLSSQKYLLGSRLCRNSSF--DDCKYDAYKTKNNLCPKSSKKCPEIINGSSSHKIWE------SQLKIVQDFCSNVTVKASCP--DLKDRINFLLFEKGNSQPIKGRSVPCNSSRGYTVAAHFRIRCRNLYQFIANLIRNTNKTEPYKKKNESILS---------QFWVFGTENCPENKFNICQSAKYRFNSTKCADGYYRC 1835
            W PN+ ++ A C NV +   CP++ K+R IE +L +      +   T  C  ++++ L +S  L C+N+Y  N+S+ P F    C  YC    SS  +L G  +C+N  F  ++   + Y T  N+CP S++KC   +   S     +      S+ K+    C N   +  CP  +   ++ F+ + + + Q        C  +    +   F + C++LY    +          +K  NESI           Q W+ G ++C E   N CQ  +Y+ N  KC     RC
Sbjct:   24 WDPNLTLISANCSNVNIEATCPKLVKERNIEFSLFEFPTQNVQSVKTYKCPASNAKKLHVSMKLPCINLYQVNISLSPIFPMINCREYCLEAHSSPIWLFGD-ICQNKQFLSNNGSLNKYMTPKNICPASTQKCSADLKTESQKSFMQTIKKTLSKAKLTDADCENAIFQMECPLFNFGSQLTFIFYRQNHFQNHSEIMKHCKPNNSNVINVSFSLSCKSLYLLHIS----------FKPTNESICDGSYSVEHPIQMWLLGGQHCFEEFENDCQYEQYKSNRNKCDISSRRC 305
[Close]
BLAST of SMED30024269 vs. Planmine SMEST
Match: SMESG000058905.1 (SMESG000058905.1)

HSP 1 Score: 210.69 bits (535), Expect = 1.616e-60
Identity = 141/449 (31.40%), Postives = 225/449 (50.11%), Query Frame = 3 
Query:  153 VPIWNPDIRLTNGVCNEVTLTAKCPTLSTRRSIEFELYPLYDPTKSV--IQTLQCRINQSQPLTTTFSIQCKNLYRVNISLSPIF---SCTEYCSDIHTSRIWLLGGNTCGE---IALDCAFNVHLPRENSCINSSIQCSLDSTVQS----ATRVSPMSLNPRLVNAYCKNVTVEASCPQSPEDQYIEFILSLSN--QKHLESISKLCQSNATKLVQVTFKISCKNFYYVEVXXXXXXXKNSKVPLRNQRS-ATWILGDVICSNDIKEKCRFIDQFKVPENKCYTSTVNCTDSSQMSLTTPSSQWSPNIKIVKAFCDNVTVSVDCPRVNKQREIEINLIK--DFRNEEFSTRICKLNSSETLEISFNLKCMNIYHFNVSMKPTFSPFECDLYCSNPLSSQKYLLGSRLCRNSSFDDCKYDAYKTKNNLCPKSSKKCPEIINGSSSHKIW 1448
            + IW+P++ L +  C+ V + A CP L   R+IEF L+    PT++V  ++T +C  + ++ L  +  + C NLY+VNISLSPIF   +C EYC + H+S IWL  G+ C     ++ + + N ++  +N C  S+ +CS D   +S       +       +L +A C+N   +  CP       + FI    N  Q H E I K C+ N + ++ V+F +SCK+ Y + +S   ++          +     W+LG   C  + +  C++ +Q+K   NKC  S+  C +          +QWSPNIK++ A+C N+TV   CP +N  R +E +L++  D       T+IC   S   L++SF  +C  +Y+ N+ M P      CD +CS   SS+ +LLG +LC     D CK   Y+   N CP     C       S  K+W
Sbjct:   22 IQIWDPNLTLISANCSNVNIEATCPKLVKERNIEFSLFEF--PTQNVQSVKTYKCPASNAKKLHVSMKLPCINLYQVNISLSPIFPMINCREYCLEAHSSPIWLF-GDICQNKQFLSNNGSLNKYMTPKNICPASTQKCSADLKTESQKSFMQTIKKTLSKAKLTDADCENAIFQMECPLFNFGSQLTFIFYRQNHFQNHSE-IMKHCKPNNSNVINVSFSLSCKSLYLLHISFKPTNESICDGSYSVEHPIQMWLLGGQHCFEEFENDCQY-EQYKSNRNKCDISSRRCREMQSF------TQWSPNIKLITAYCTNITVQATCPFLNVSRNLEFSLLRMGDRSISATVTKICNPLSKTYLKVSFEFECRAVYYVNIIMSPITPSMICDEFCSKQHSSEMWLLGRKLCPKVGLDYCKVRKYEINENHCPIHLDTCKSF---QSYKKVW 456

HSP 2 Score: 150.214 bits (378), Expect = 8.837e-39
Identity = 113/419 (26.97%), Postives = 195/419 (46.54%), Query Frame = 3 
Query:  597 NPRLVNAYCKNVTVEASCPQSPEDQYIEFILSLSNQKHLESISKL-CQSNATKLVQVTFKISCKNFYYVEVXXXXX--XXKNSKVPLRNQRSATWILGDVICSNDIKEKCRFIDQFKVPENKCYTSTVNCT----DSSQMSLTTPSSQWSPNIKIVKAFCDNVTVSVDCPRVNKQREIEINLIKD--FRNEEFSTRICKLNSSETLEISFNLKCMNIYHFNVSMKPTFSPFECDLYCSNPLSSQKYLLGSRLCRNSSFDDCKYDAYKTKNNLCPKSSKKCPEIINGSSSHKIWESQLKIVQDFCSNVTVKASCP--DLKDRINFLLFEKGNSQPIKGRSVPCNSSRGYTVAAHFRIRCRNLY--QFIANLIRNTNKTEPYKKKNESILSQFWVFGTENCPENKFNICQSAKYRFNSTKC 1814
            N  L++A C NV +EA+CP+  +++ IEF L     ++++S+    C ++  K + V+ K+ C N Y V +SL      +   +  L    S  W+ GD+  +         ++++  P+N C  ST  C+      SQ S      +     K+  A C+N    ++CP  N   ++     +   F+N     + CK N+S  + +SF+L C ++Y  ++S KPT     CD   S     Q +LLG + C     +DC+Y+ YK+  N C  SS++C E+     S   W   +K++  +C+N+TV+A+CP  ++   + F L   G+       +  CN      +   F   CR +Y    I + I  +   + +  K  S  S+ W+ G + CP+   + C+  KY  N   C
Sbjct:   28 NLTLISANCSNVNIEATCPKLVKERNIEFSLFEFPTQNVQSVKTYKCPASNAKKLHVSMKLPCINLYQVNISLSPIFPMINCREYCLEAHSSPIWLFGDICQNKQFLSNNGSLNKYMTPKNICPASTQKCSADLKTESQKSFMQTIKKTLSKAKLTDADCENAIFQMECPLFNFGSQLTFIFYRQNHFQNHSEIMKHCKPNNSNVINVSFSLSCKSLYLLHISFKPTNESI-CDGSYSVEHPIQMWLLGGQHCFEEFENDCQYEQYKSNRNKCDISSRRCREM----QSFTQWSPNIKLITAYCTNITVQATCPFLNVSRNLEFSLLRMGDRSISATVTKICNPLSKTYLKVSFEFECRAVYYVNIIMSPITPSMICDEFCSKQHS--SEMWLLGRKLCPKVGLDYCKVRKYEINENHC 439

HSP 3 Score: 113.62 bits (283), Expect = 2.303e-26
Identity = 77/293 (26.28%), Postives = 129/293 (44.03%), Query Frame = 3 
Query: 1020 WSPNIKIVKAFCDNVTVSVDCPRVNKQREIEINLIK--DFRNEEFSTRICKLNSSETLEISFNLKCMNIYHFNVSMKPTFSPFECDLYCSNPLSSQKYLLGSRLCRNSSF--DDCKYDAYKTKNNLCPKSSKKCPEIINGSSSHKIWE------SQLKIVQDFCSNVTVKASCP--DLKDRINFLLFEKGNSQPIKGRSVPCNSSRGYTVAAHFRIRCRNLYQFIANLIRNTNKTEPYKKKNESILS---------QFWVFGTENCPENKFNICQSAKYRFNSTKCADGYYRC 1835
            W PN+ ++ A C NV +   CP++ K+R IE +L +      +   T  C  ++++ L +S  L C+N+Y  N+S+ P F    C  YC    SS  +L G  +C+N  F  ++   + Y T  N+CP S++KC   +   S     +      S+ K+    C N   +  CP  +   ++ F+ + + + Q        C  +    +   F + C++LY    +          +K  NESI           Q W+ G ++C E   N CQ  +Y+ N  KC     RC
Sbjct:   25 WDPNLTLISANCSNVNIEATCPKLVKERNIEFSLFEFPTQNVQSVKTYKCPASNAKKLHVSMKLPCINLYQVNISLSPIFPMINCREYCLEAHSSPIWLFGD-ICQNKQFLSNNGSLNKYMTPKNICPASTQKCSADLKTESQKSFMQTIKKTLSKAKLTDADCENAIFQMECPLFNFGSQLTFIFYRQNHFQNHSEIMKHCKPNNSNVINVSFSLSCKSLYLLHIS----------FKPTNESICDGSYSVEHPIQMWLLGGQHCFEEFENDCQYEQYKSNRNKCDISSRRC 306
[Close]
BLAST of SMED30024269 vs. Planmine SMEST
Match: SMESG000062659.1 (SMESG000062659.1)

HSP 1 Score: 78.9518 bits (193), Expect = 3.583e-16
Identity = 43/138 (31.16%), Postives = 72/138 (52.17%), Query Frame = 3 
Query:  999 LTTPSSQWSPNIKIVKAFCDNVTVSVDCPRVNKQREIEINLIKDFRNEEFS----TRICKLNSSETLEISFNLKCMNIYHFNVSMKPTFSPFECDLYCSNPLSSQKYLLGSRLCRNSSFDDCKYDAYKTKNNLCPKSS 1400
             T+  + WSP++K+  A C   T+   CP   +   ++I+LI    N+E       R C+L+ S+ ++  F++KC  +Y   V++ P  +   C+  CS  L S  + LG   C  +  +D +Y+ YKT  N+CP SS
Sbjct:   15 FTSAFATWSPDMKLHSANCQTATIETSCPNNTEDYMLDISLITFVNNQELQLENVMRTCRLSKSKLIQTHFSVKCKGLYQVKVNLVPK-NLSNCNKDCSKTLKSSVWYLGELACDEAQSNDYQYEKYKTTKNICPASS 151

HSP 2 Score: 65.855 bits (159), Expect = 8.590e-12
Identity = 48/182 (26.37%), Postives = 88/182 (48.35%), Query Frame = 3 
Query:   99 IYLINLFIFYICCNWVSLVPIWNPDIRLTNGVCNEVTLTAKCPTLSTRRSIEFELYPLYDPTKSVIQTLQ--CRINQSQPLTTTFSIQCKNLYRVNISLSP--IFSCTEYCSDIHTSRIWLLGGNTCGEI-ALDCAFNVHLPRENSCINSSIQCSLDSTVQSATRVSPMSLNPRLVNAYCKN 629
            I+L++LF         S    W+PD++L +  C   T+   CP  +    ++  L    +  +  ++ +   CR+++S+ + T FS++CK LY+V ++L P  + +C + CS    S +W LG   C E  + D  +  +   +N C  SSI+ +++       R  P    PR  N+  +N
Sbjct:    9 IFLLHLF--------TSAFATWSPDMKLHSANCQTATIETSCPNNTEDYMLDISLITFVNNQELQLENVMRTCRLSKSKLIQTHFSVKCKGLYQVKVNLVPKNLSNCNKDCSKTLKSSVWYLGELACDEAQSNDYQYEKYKTTKNICPASSIRLTVNH------RQVPAIKQPRETNSKVEN 176

HSP 3 Score: 58.151 bits (139), Expect = 3.562e-9
Identity = 40/130 (30.77%), Postives = 74/130 (56.92%), Query Frame = 3 
Query:  603 RLVNAYCKNVTVEASCPQSPEDQY--IEFILSLSNQK-HLESISKLCQSNATKLVQVTFKISCKNFYYVEVXXXXXXXKN-SKVPLRNQRSATWILGDVICSNDIKEKCRFIDQFKVPENKCYTSTVNCT 980
            +L +A C+  T+E SCP + ED    I  I  ++NQ+  LE++ + C+ + +KL+Q  F + CK  Y V+V+L+  +L N +K   +  +S+ W LG++ C        ++ +++K  +N C  S++  T
Sbjct:   27 KLHSANCQTATIETSCPNNTEDYMLDISLITFVNNQELQLENVMRTCRLSKSKLIQTHFSVKCKGLYQVKVNLVPKNLSNCNKDCSKTLKSSVWYLGELACDEAQSNDYQY-EKYKTTKNICPASSIRLT 155

HSP 4 Score: 49.2914 bits (116), Expect = 2.918e-6
Identity = 34/141 (24.11%), Postives = 58/141 (41.13%), Query Frame = 3 
Query: 1428 SSSHKIWESQLKIVQDFCSNVTVKASCP----DLKDRINFLLFEKGNSQPIKGRSVPCNSSRGYTVAAHFRIRCRNLYQFIANLIRNTNKTEPYKKKNESILSQFWVFGTENCPENKFNICQSAKYRFNSTKCADGYYRCS 1838
            +S+   W   +K+    C   T++ SCP    D    I+ + F       ++     C  S+   +  HF ++C+ LYQ   NL+   N +   K  ++++ S  W  G   C E + N  Q  KY+     C     R +
Sbjct:   16 TSAFATWSPDMKLHSANCQTATIETSCPNNTEDYMLDISLITFVNNQELQLENVMRTCRLSKSKLIQTHFSVKCKGLYQVKVNLVPK-NLSNCNKDCSKTLKSSVWYLGELACDEAQSNDYQYEKYKTTKNICPASSIRLT 155
The following BLAST results are available for this feature:
BLAST of SMED30024269 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30024269 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30024269 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30024269 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30024269 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30024269 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30024269 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30024269 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30024269 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30024269 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30024269 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30024269 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30024269 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30024269 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000058883.10.000e+0100.00SMESG000058883.1[more]
SMESG000058905.11.076e-6031.40SMESG000058905.1[more]
SMESG000058905.11.613e-6031.40SMESG000058905.1[more]
SMESG000058905.11.616e-6031.40SMESG000058905.1[more]
SMESG000062659.13.583e-1631.16SMESG000062659.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024269 ID=SMED30024269|Name=SMED30024269|organism=Schmidtea mediterranea sexual|type=transcript|length=1928bp
CAAAAAAGCATTTTGACATGATTCATTTCATTATTTGCTTGATTTATTTA
TTGTTTTGCAATTTGGTTGAAAATGCGACTGCACTCGTTTGGTATATGAT
TTATTTAATAAATTTATTTATATTCTATATTTGCTGCAACTGGGTATCCT
TAGTGCCGATTTGGAATCCCGATATTCGACTTACAAATGGTGTTTGCAAT
GAAGTTACTTTGACTGCGAAATGTCCAACATTGTCGACAAGGAGAAGCAT
TGAATTCGAATTGTATCCATTGTATGACCCTACAAAGTCTGTCATACAAA
CTCTGCAGTGTAGAATAAACCAAAGTCAACCTCTCACAACGACATTCTCA
ATCCAATGCAAGAACTTATATCGAGTGAATATCAGCCTTTCACCAATATT
TTCATGCACTGAGTACTGTTCCGATATCCACACAAGCAGGATTTGGCTTT
TAGGCGGTAACACTTGCGGAGAAATCGCATTAGATTGTGCATTCAATGTG
CATTTACCACGTGAGAACTCTTGTATAAATTCATCCATCCAATGCTCTTT
GGATTCTACAGTTCAATCTGCCACAAGAGTGTCACCGATGTCTTTGAATC
CGAGGTTAGTGAATGCATACTGCAAAAATGTCACGGTTGAAGCTAGTTGT
CCACAGTCACCTGAGGATCAGTACATTGAATTTATCTTAAGTCTTTCTAA
TCAAAAACACCTGGAAAGTATTTCCAAACTTTGTCAATCTAATGCTACAA
AATTAGTTCAAGTGACATTCAAAATTTCTTGTAAAAACTTTTATTACGTT
GAAGTAAGTTTACTTTCTTCTTCTCTCAAAAATTCAAAAGTCCCTCTAAG
AAATCAAAGAAGTGCAACATGGATATTAGGAGATGTAATATGCTCCAATG
ACATCAAAGAAAAATGTCGATTTATTGACCAGTTTAAAGTCCCAGAAAAC
AAATGTTACACGTCAACCGTTAATTGTACTGATTCTAGTCAAATGTCTCT
AACGACTCCCTCATCACAGTGGTCACCTAATATTAAAATTGTAAAAGCCT
TTTGCGACAATGTAACCGTATCAGTCGACTGTCCTAGGGTGAACAAACAG
AGAGAAATTGAAATTAATTTAATCAAAGATTTTCGGAATGAAGAATTTTC
AACAAGAATTTGTAAATTGAACAGTTCAGAAACTTTGGAAATATCTTTCA
ATTTGAAATGCATGAATATTTACCATTTTAATGTCAGCATGAAACCCACG
TTTTCCCCATTCGAATGTGACCTGTACTGCAGTAATCCTCTATCAAGTCA
AAAGTATTTATTGGGATCTAGGCTATGCCGAAATTCCTCATTTGATGACT
GCAAATATGATGCCTACAAAACAAAGAATAACCTCTGCCCGAAGTCATCC
AAGAAATGCCCTGAAATCATAAATGGATCAAGCAGCCATAAAATTTGGGA
ATCACAATTGAAAATCGTCCAAGACTTTTGCAGTAACGTAACGGTTAAAG
CCAGTTGCCCGGATTTAAAAGATAGGATTAATTTCTTACTTTTTGAAAAA
GGAAATTCCCAACCGATCAAAGGAAGGTCTGTGCCATGTAATTCTAGTAG
AGGGTATACTGTGGCTGCTCATTTTAGGATTAGGTGTAGAAACCTCTACC
AATTCATAGCAAATTTGATTCGAAATACAAATAAAACTGAGCCCTATAAA
AAGAAAAACGAGTCGATTCTATCTCAATTTTGGGTTTTTGGCACAGAAAA
TTGCCCGGAAAACAAGTTTAATATTTGCCAAAGTGCCAAATATAGATTTA
ACAGTACTAAATGTGCGGACGGATATTACAGATGTTCAAAACCAGTTGAC
GGAAAAAGTCTTGGAGTTTTGATTTTCTTGGTCGGGCTTCTGTTATGTAT
CATATGTATAAGTATAATTATATTTTAT
back to top

protein sequence of SMED30024269-orf-1

>SMED30024269-orf-1 ID=SMED30024269-orf-1|Name=SMED30024269-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=642bp
KKHFDMIHFIICLIYLLFCNLVENATALVWYMIYLINLFIFYICCNWVSL
VPIWNPDIRLTNGVCNEVTLTAKCPTLSTRRSIEFELYPLYDPTKSVIQT
LQCRINQSQPLTTTFSIQCKNLYRVNISLSPIFSCTEYCSDIHTSRIWLL
GGNTCGEIALDCAFNVHLPRENSCINSSIQCSLDSTVQSATRVSPMSLNP
RLVNAYCKNVTVEASCPQSPEDQYIEFILSLSNQKHLESISKLCQSNATK
LVQVTFKISCKNFYYVEVSLLSSSLKNSKVPLRNQRSATWILGDVICSND
IKEKCRFIDQFKVPENKCYTSTVNCTDSSQMSLTTPSSQWSPNIKIVKAF
CDNVTVSVDCPRVNKQREIEINLIKDFRNEEFSTRICKLNSSETLEISFN
LKCMNIYHFNVSMKPTFSPFECDLYCSNPLSSQKYLLGSRLCRNSSFDDC
KYDAYKTKNNLCPKSSKKCPEIINGSSSHKIWESQLKIVQDFCSNVTVKA
SCPDLKDRINFLLFEKGNSQPIKGRSVPCNSSRGYTVAAHFRIRCRNLYQ
FIANLIRNTNKTEPYKKKNESILSQFWVFGTENCPENKFNICQSAKYRFN
STKCADGYYRCSKPVDGKSLGVLIFLVGLLLCIICISIIIFY
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0002109X1 cell
PLANA:0002111X2 cell