Protein phosphatase 1 regulatory subunit 3A

Overview
NameProtein phosphatase 1 regulatory subunit 3A
Smed IDSMED30024231
Length (bp)1439
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Protein phosphatase 1 regulatory subunit 3A (SMED30024231) t-SNE clustered cells

Violin plots show distribution of expression levels for Protein phosphatase 1 regulatory subunit 3A (SMED30024231) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Protein phosphatase 1 regulatory subunit 3A (SMED30024231) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Protein phosphatase 1 regulatory subunit 3A (SMED30024231) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30024231

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
protonephridiaSMED30024231h1SMcG0004471 dd_Smed_v4_4959_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30024231h1SMcG0004471 dd_Smed_v4_4959_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30024231h1SMcG0004471 dd_Smed_v4_4959_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30024231h1SMcG0004471 dd_Smed_v4_4959_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30024231h1SMcG0004471 OX_Smed_1.0.07010ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Human
Match: PPP1R3B (protein phosphatase 1 regulatory subunit 3B [Source:HGNC Symbol;Acc:HGNC:14942])

HSP 1 Score: 109.383 bits (272), Expect = 3.290e-26
Identity = 65/175 (37.14%), Postives = 103/175 (58.86%), Query Frame = 1
Query:  403 KAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGA-ASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGV-TDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNY 921
            K V FAD  GL L  ++     D P  +P +  + L       TA +E   ++  +F QP A   DF  R++  +V LEN   ++  ++GT++V N++FEK V +R+TF+ W+S+ DF   YV+D++ G   D FSF   LP + Q S +R++FA+ +EC+   T+WD+N G+NY
Sbjct:   61 KRVSFADNQGLALTMVKVFSEFDDPLDMPFNITELLDNIVSLTTAESE---SFVLDFSQPSADYLDFRNRLQADHVCLENCVLKDKAIAGTVKVQNLAFEKTVKIRMTFDTWKSYTDFPCQYVKDTYAGSDRDTFSFDISLPEKIQ-SYERMEFAVYYECN-GQTYWDSNRGKNY 230          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Human
Match: PPP1R3B (protein phosphatase 1 regulatory subunit 3B [Source:HGNC Symbol;Acc:HGNC:14942])

HSP 1 Score: 109.383 bits (272), Expect = 3.290e-26
Identity = 65/175 (37.14%), Postives = 103/175 (58.86%), Query Frame = 1
Query:  403 KAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGA-ASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGV-TDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNY 921
            K V FAD  GL L  ++     D P  +P +  + L       TA +E   ++  +F QP A   DF  R++  +V LEN   ++  ++GT++V N++FEK V +R+TF+ W+S+ DF   YV+D++ G   D FSF   LP + Q S +R++FA+ +EC+   T+WD+N G+NY
Sbjct:   61 KRVSFADNQGLALTMVKVFSEFDDPLDMPFNITELLDNIVSLTTAESE---SFVLDFSQPSADYLDFRNRLQADHVCLENCVLKDKAIAGTVKVQNLAFEKTVKIRMTFDTWKSYTDFPCQYVKDTYAGSDRDTFSFDISLPEKIQ-SYERMEFAVYYECN-GQTYWDSNRGKNY 230          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Human
Match: PPP1R3B (protein phosphatase 1 regulatory subunit 3B [Source:HGNC Symbol;Acc:HGNC:14942])

HSP 1 Score: 109.383 bits (272), Expect = 3.290e-26
Identity = 65/175 (37.14%), Postives = 103/175 (58.86%), Query Frame = 1
Query:  403 KAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGA-ASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGV-TDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNY 921
            K V FAD  GL L  ++     D P  +P +  + L       TA +E   ++  +F QP A   DF  R++  +V LEN   ++  ++GT++V N++FEK V +R+TF+ W+S+ DF   YV+D++ G   D FSF   LP + Q S +R++FA+ +EC+   T+WD+N G+NY
Sbjct:   61 KRVSFADNQGLALTMVKVFSEFDDPLDMPFNITELLDNIVSLTTAESE---SFVLDFSQPSADYLDFRNRLQADHVCLENCVLKDKAIAGTVKVQNLAFEKTVKIRMTFDTWKSYTDFPCQYVKDTYAGSDRDTFSFDISLPEKIQ-SYERMEFAVYYECN-GQTYWDSNRGKNY 230          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Human
Match: PPP1R3B (protein phosphatase 1 regulatory subunit 3B [Source:HGNC Symbol;Acc:HGNC:14942])

HSP 1 Score: 109.383 bits (272), Expect = 3.290e-26
Identity = 65/175 (37.14%), Postives = 103/175 (58.86%), Query Frame = 1
Query:  403 KAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGA-ASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGV-TDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNY 921
            K V FAD  GL L  ++     D P  +P +  + L       TA +E   ++  +F QP A   DF  R++  +V LEN   ++  ++GT++V N++FEK V +R+TF+ W+S+ DF   YV+D++ G   D FSF   LP + Q S +R++FA+ +EC+   T+WD+N G+NY
Sbjct:   61 KRVSFADNQGLALTMVKVFSEFDDPLDMPFNITELLDNIVSLTTAESE---SFVLDFSQPSADYLDFRNRLQADHVCLENCVLKDKAIAGTVKVQNLAFEKTVKIRMTFDTWKSYTDFPCQYVKDTYAGSDRDTFSFDISLPEKIQ-SYERMEFAVYYECN-GQTYWDSNRGKNY 230          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Human
Match: PPP1R3D (protein phosphatase 1 regulatory subunit 3D [Source:HGNC Symbol;Acc:HGNC:9294])

HSP 1 Score: 108.227 bits (269), Expect = 1.005e-25
Identity = 77/258 (29.84%), Postives = 122/258 (47.29%), Query Frame = 1
Query:  217 ISPVDSVASSNGSECRWIGNSKFHQEKSSDSETNTDSEFKYYKPKRASSL--------KTNRTP-----PGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYR-----ANFEQPGAASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDS-HDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTC 933
            +S +D   +     CR  G S       + + +  D   +    +RA SL        K    P     PG  +   VRFADA GL+L  ++ VFN    P +P   +  L ++SD     + + L +       +F  P  A+DF  R++   V LE     +  +SGT+RV N++FEK+V VR TF+ WRS  +  A +   +  +G  D F+F F +P      G R+ FA+ ++ +  + +WDNN  ++Y  TC
Sbjct:   27 LSDLDGGVALEPRACRPPG-SPGRAPPPTPAPSGCDPRLRPIILRRARSLPSSPERRQKAAGAPGAACRPGCSQKLRVRFADALGLELAQVK-VFNAGDDPSVPLHVLSRLAINSD--LCCSSQDLEFTLHCLVPDFPPPVEAADFGERLQRQLVCLERVTCSDLGISGTVRVCNVAFEKQVAVRYTFSGWRSTHEAVARWRGPAGPEGTEDVFTFGFPVPPFLLELGSRVHFAVRYQVA-GAEYWDNNDHRDYSLTC 279          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Celegans
Match: H18N23.2 (Protein phosphatase 1 regulatory subunit [Source:UniProtKB/TrEMBL;Acc:Q400N3])

HSP 1 Score: 90.5077 bits (223), Expect = 1.082e-19
Identity = 65/187 (34.76%), Postives = 98/187 (52.41%), Query Frame = 1
Query:  403 KAVRFADAFGLDLEDIRHVFNL-DLPPKIPASAVQDLVLDS--DSDTAINEKGLNYRANFEQPGAASDFLR---RVRMHNVALENAFFENNF--LSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTCSK 939
            K+VRFAD  G +L  +R      + PPK+  S ++    +S  + +    E    +   F+QP  A+D++R    +  + V+LEN    N    + GTI+V NI+FEK V VR T N W S++D  A Y Q S   + D F F   LP+  +   Q I+F I F+ +  + +WD+N G NYV  C +
Sbjct:  115 KSVRFADDCGHELYMVRVATEPSNCPPKLSPSVLRRYRGESFEEEEMTTREPAPVWNLMFKQP--AADYVRFRENMETNKVSLENVILNNEAYKVMGTIKVGNIAFEKSVFVRYTMNGWISYMDKAAIY-QPSTSKIQDTFKFDLDLPSSVEKIHQ-IEFCICFK-ANGTEYWDSNSGTNYVLHCEQ 296          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Celegans
Match: H18N23.2 (Protein phosphatase 1 regulatory subunit [Source:UniProtKB/TrEMBL;Acc:Q400N3])

HSP 1 Score: 90.5077 bits (223), Expect = 1.168e-19
Identity = 65/187 (34.76%), Postives = 98/187 (52.41%), Query Frame = 1
Query:  403 KAVRFADAFGLDLEDIRHVFNL-DLPPKIPASAVQDLVLDS--DSDTAINEKGLNYRANFEQPGAASDFLR---RVRMHNVALENAFFENNF--LSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTCSK 939
            K+VRFAD  G +L  +R      + PPK+  S ++    +S  + +    E    +   F+QP  A+D++R    +  + V+LEN    N    + GTI+V NI+FEK V VR T N W S++D  A Y Q S   + D F F   LP+  +   Q I+F I F+ +  + +WD+N G NYV  C +
Sbjct:  125 KSVRFADDCGHELYMVRVATEPSNCPPKLSPSVLRRYRGESFEEEEMTTREPAPVWNLMFKQP--AADYVRFRENMETNKVSLENVILNNEAYKVMGTIKVGNIAFEKSVFVRYTMNGWISYMDKAAIY-QPSTSKIQDTFKFDLDLPSSVEKIHQ-IEFCICFK-ANGTEYWDSNSGTNYVLHCEQ 306          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Celegans
Match: H18N23.2 (Protein phosphatase 1 regulatory subunit [Source:UniProtKB/TrEMBL;Acc:Q400N3])

HSP 1 Score: 89.7373 bits (221), Expect = 2.670e-19
Identity = 65/187 (34.76%), Postives = 98/187 (52.41%), Query Frame = 1
Query:  403 KAVRFADAFGLDLEDIRHVFNL-DLPPKIPASAVQDLVLDS--DSDTAINEKGLNYRANFEQPGAASDFLR---RVRMHNVALENAFFENNF--LSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTCSK 939
            K+VRFAD  G +L  +R      + PPK+  S ++    +S  + +    E    +   F+QP  A+D++R    +  + V+LEN    N    + GTI+V NI+FEK V VR T N W S++D  A Y Q S   + D F F   LP+  +   Q I+F I F+ +  + +WD+N G NYV  C +
Sbjct:  137 KSVRFADDCGHELYMVRVATEPSNCPPKLSPSVLRRYRGESFEEEEMTTREPAPVWNLMFKQP--AADYVRFRENMETNKVSLENVILNNEAYKVMGTIKVGNIAFEKSVFVRYTMNGWISYMDKAAIY-QPSTSKIQDTFKFDLDLPSSVEKIHQ-IEFCICFK-ANGTEYWDSNSGTNYVLHCEQ 318          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Celegans
Match: CC8.2 (Protein phosphatase 1 regulatory subunit [Source:UniProtKB/TrEMBL;Acc:H2KZH6])

HSP 1 Score: 59.3066 bits (142), Expect = 1.589e-9
Identity = 48/188 (25.53%), Postives = 88/188 (46.81%), Query Frame = 1
Query:  403 KAVRFADAFGLDLEDIRHVFNLDLPPK-------IPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGAASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQ---DSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTCS 936
            K VRFAD+ GLDLE++++    DL  +        P+S+     L       IN+       +     +  ++  + R   V +       + + G + ++N++F+K V VR T + W +  +  A+Y     D+ D   D F+F+  +P   + S  + +F + ++ +  + FWDNN G NY+   S
Sbjct:   54 KRVRFADSLGLDLENLQYFVKEDLHLQKFNIHFSTPSSSTPPPQLPHFPKQLINQPAPRLVLHNFPYRSEQEYQVKTRDSRVCVAALRASGSSIVGQVNLLNVAFDKVVVVRYTIDGWATQDEIVASYSHRLFDTED--IDAFNFTIAIPV--KLSEGKCEFCVQYQVA-GAEFWDNNGGDNYIVNVS 236          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Celegans
Match: CC8.2 (Protein phosphatase 1 regulatory subunit [Source:UniProtKB/TrEMBL;Acc:H2KZH6])

HSP 1 Score: 58.9214 bits (141), Expect = 2.187e-9
Identity = 48/188 (25.53%), Postives = 88/188 (46.81%), Query Frame = 1
Query:  403 KAVRFADAFGLDLEDIRHVFNLDLPPK-------IPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGAASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQ---DSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTCS 936
            K VRFAD+ GLDLE++++    DL  +        P+S+     L       IN+       +     +  ++  + R   V +       + + G + ++N++F+K V VR T + W +  +  A+Y     D+ D   D F+F+  +P   + S  + +F + ++ +  + FWDNN G NY+   S
Sbjct:   77 KRVRFADSLGLDLENLQYFVKEDLHLQKFNIHFSTPSSSTPPPQLPHFPKQLINQPAPRLVLHNFPYRSEQEYQVKTRDSRVCVAALRASGSSIVGQVNLLNVAFDKVVVVRYTIDGWATQDEIVASYSHRLFDTED--IDAFNFTIAIPV--KLSEGKCEFCVQYQVA-GAEFWDNNGGDNYIVNVS 259          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Fly
Match: Gbs-76A (gene:FBgn0036862 transcript:FBtr0331425)

HSP 1 Score: 161.384 bits (407), Expect = 9.042e-43
Identity = 89/201 (44.28%), Postives = 122/201 (60.70%), Query Frame = 1
Query:  349 KRASSLKTNRTPPGTP-KSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDL-VLDSDSDTAINEKGLNYRAN-FEQPGAASDFLRRVRMHNVALENAFFENNF---LSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTC 933
            +R SSLKT +TPPGTP + K VRFAD  GLDL D++    LD  P IP SA +DL +L+S+    +  K        F+QPG    FL  VR   V+LENA   +N    +SG++RV N+ F K V +R + + WRS+ D  ANYV++S DG +D   F+F+L       GQR++FA+ F+C +   FWDNN+G NY + C
Sbjct:  438 RRCSSLKTGKTPPGTPGRKKIVRFADVLGLDLADVKTF--LDEIPTIPKSAFEDLEILESEPPLQLGPKSDKLLMPLFQQPGGLPKFLDAVREKQVSLENAAVTDNINQTISGSVRVRNLDFHKSVHIRYSLDGWRSYADLQANYVENSCDGFSD--IFTFVLFGNSLHVGQRLEFAVRFQC-KGQQFWDNNYGANYCFQC 633          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Fly
Match: Gbs-76A (gene:FBgn0036862 transcript:FBtr0075014)

HSP 1 Score: 161.384 bits (407), Expect = 9.042e-43
Identity = 89/201 (44.28%), Postives = 122/201 (60.70%), Query Frame = 1
Query:  349 KRASSLKTNRTPPGTP-KSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDL-VLDSDSDTAINEKGLNYRAN-FEQPGAASDFLRRVRMHNVALENAFFENNF---LSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTC 933
            +R SSLKT +TPPGTP + K VRFAD  GLDL D++    LD  P IP SA +DL +L+S+    +  K        F+QPG    FL  VR   V+LENA   +N    +SG++RV N+ F K V +R + + WRS+ D  ANYV++S DG +D   F+F+L       GQR++FA+ F+C +   FWDNN+G NY + C
Sbjct:  438 RRCSSLKTGKTPPGTPGRKKIVRFADVLGLDLADVKTF--LDEIPTIPKSAFEDLEILESEPPLQLGPKSDKLLMPLFQQPGGLPKFLDAVREKQVSLENAAVTDNINQTISGSVRVRNLDFHKSVHIRYSLDGWRSYADLQANYVENSCDGFSD--IFTFVLFGNSLHVGQRLEFAVRFQC-KGQQFWDNNYGANYCFQC 633          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Fly
Match: Gbs-76A (gene:FBgn0036862 transcript:FBtr0345390)

HSP 1 Score: 161.384 bits (407), Expect = 9.042e-43
Identity = 89/201 (44.28%), Postives = 122/201 (60.70%), Query Frame = 1
Query:  349 KRASSLKTNRTPPGTP-KSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDL-VLDSDSDTAINEKGLNYRAN-FEQPGAASDFLRRVRMHNVALENAFFENNF---LSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTC 933
            +R SSLKT +TPPGTP + K VRFAD  GLDL D++    LD  P IP SA +DL +L+S+    +  K        F+QPG    FL  VR   V+LENA   +N    +SG++RV N+ F K V +R + + WRS+ D  ANYV++S DG +D   F+F+L       GQR++FA+ F+C +   FWDNN+G NY + C
Sbjct:  438 RRCSSLKTGKTPPGTPGRKKIVRFADVLGLDLADVKTF--LDEIPTIPKSAFEDLEILESEPPLQLGPKSDKLLMPLFQQPGGLPKFLDAVREKQVSLENAAVTDNINQTISGSVRVRNLDFHKSVHIRYSLDGWRSYADLQANYVENSCDGFSD--IFTFVLFGNSLHVGQRLEFAVRFQC-KGQQFWDNNYGANYCFQC 633          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Fly
Match: Gbs-76A (gene:FBgn0036862 transcript:FBtr0332155)

HSP 1 Score: 161.384 bits (407), Expect = 9.042e-43
Identity = 89/201 (44.28%), Postives = 122/201 (60.70%), Query Frame = 1
Query:  349 KRASSLKTNRTPPGTP-KSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDL-VLDSDSDTAINEKGLNYRAN-FEQPGAASDFLRRVRMHNVALENAFFENNF---LSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTC 933
            +R SSLKT +TPPGTP + K VRFAD  GLDL D++    LD  P IP SA +DL +L+S+    +  K        F+QPG    FL  VR   V+LENA   +N    +SG++RV N+ F K V +R + + WRS+ D  ANYV++S DG +D   F+F+L       GQR++FA+ F+C +   FWDNN+G NY + C
Sbjct:  438 RRCSSLKTGKTPPGTPGRKKIVRFADVLGLDLADVKTF--LDEIPTIPKSAFEDLEILESEPPLQLGPKSDKLLMPLFQQPGGLPKFLDAVREKQVSLENAAVTDNINQTISGSVRVRNLDFHKSVHIRYSLDGWRSYADLQANYVENSCDGFSD--IFTFVLFGNSLHVGQRLEFAVRFQC-KGQQFWDNNYGANYCFQC 633          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Fly
Match: Gbs-70E (gene:FBgn0036428 transcript:FBtr0075723)

HSP 1 Score: 82.8037 bits (203), Expect = 4.821e-17
Identity = 64/192 (33.33%), Postives = 97/192 (50.52%), Query Frame = 1
Query:  394 PKSKAVRFADAFGLDLEDIRHVFNL-DLPP----KIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGAASDFL---RRVRMHNVALENAFF--ENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDG-----VTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYV 924
            PK K V FAD  GL L ++R +    ++PP    K      Q LV     D         +  +F+QP  ASD+L   +++    V+LEN     E + + GTI+V NI F+K++ VR+T++DW+S  D    Y +          V D FSF   LP     S +R++F I +  +++  +WDNN G+NY 
Sbjct:  102 PK-KHVIFADDEGLSLTEVRVMSEPSNVPPYWSMKFLEQITQGLVSPHPPD--------QWTVDFKQP--ASDYLSFRQKIERDFVSLENVIVKDEESIVVGTIKVKNIDFQKEIIVRVTWDDWKSQQDIFCTYARAYGPATCAHVVFDTFSFKITLPP----SSKRLEFCICYRTNETE-YWDNNDGKNYT 277          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Zebrafish
Match: ppp1r3b (protein phosphatase 1, regulatory subunit 3B [Source:NCBI gene;Acc:327285])

HSP 1 Score: 105.145 bits (261), Expect = 7.734e-25
Identity = 74/196 (37.76%), Postives = 103/196 (52.55%), Query Frame = 1
Query:  385 PGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGAASDFLR---RVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVT-DRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTCSKDFKSFND 960
            PG  K K V FAD  GL L  ++     D P  IP +          S   ++E       +F+QP A  D+L+   R+   +V LE+   +   + GT++V NISFEK V +RITFN W++H D    YV+D++ G   D FSF   LP +   S +RI+FAI +E +   T WDNN G+NY    S   KS N+
Sbjct:   61 PGRAK-KHVSFADHKGLALTVVKIFSEFDDPIDIPDNIDNFFT----SSLTVSEGKDKLTLDFDQPSA--DYLKFRQRIENDHVCLEHCMLKEKSIMGTVKVKNISFEKSVKLRITFNTWKNHTDVECQYVKDTYTGSNRDTFSFEASLPEQV-PSHERIEFAICYEVN-GDTLWDNNQGKNYRIIQSALRKSSNE 247          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Zebrafish
Match: ppp1r3b (protein phosphatase 1, regulatory subunit 3B [Source:NCBI gene;Acc:327285])

HSP 1 Score: 105.145 bits (261), Expect = 7.734e-25
Identity = 74/196 (37.76%), Postives = 103/196 (52.55%), Query Frame = 1
Query:  385 PGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGAASDFLR---RVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVT-DRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTCSKDFKSFND 960
            PG  K K V FAD  GL L  ++     D P  IP +          S   ++E       +F+QP A  D+L+   R+   +V LE+   +   + GT++V NISFEK V +RITFN W++H D    YV+D++ G   D FSF   LP +   S +RI+FAI +E +   T WDNN G+NY    S   KS N+
Sbjct:   61 PGRAK-KHVSFADHKGLALTVVKIFSEFDDPIDIPDNIDNFFT----SSLTVSEGKDKLTLDFDQPSA--DYLKFRQRIENDHVCLEHCMLKEKSIMGTVKVKNISFEKSVKLRITFNTWKNHTDVECQYVKDTYTGSNRDTFSFEASLPEQV-PSHERIEFAICYEVN-GDTLWDNNQGKNYRIIQSALRKSSNE 247          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Zebrafish
Match: ppp1r3b (protein phosphatase 1, regulatory subunit 3B [Source:NCBI gene;Acc:327285])

HSP 1 Score: 105.145 bits (261), Expect = 7.734e-25
Identity = 74/196 (37.76%), Postives = 103/196 (52.55%), Query Frame = 1
Query:  385 PGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGAASDFLR---RVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVT-DRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTCSKDFKSFND 960
            PG  K K V FAD  GL L  ++     D P  IP +          S   ++E       +F+QP A  D+L+   R+   +V LE+   +   + GT++V NISFEK V +RITFN W++H D    YV+D++ G   D FSF   LP +   S +RI+FAI +E +   T WDNN G+NY    S   KS N+
Sbjct:   61 PGRAK-KHVSFADHKGLALTVVKIFSEFDDPIDIPDNIDNFFT----SSLTVSEGKDKLTLDFDQPSA--DYLKFRQRIENDHVCLEHCMLKEKSIMGTVKVKNISFEKSVKLRITFNTWKNHTDVECQYVKDTYTGSNRDTFSFEASLPEQV-PSHERIEFAICYEVN-GDTLWDNNQGKNYRIIQSALRKSSNE 247          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Zebrafish
Match: ppp1r3b (protein phosphatase 1, regulatory subunit 3B [Source:NCBI gene;Acc:327285])

HSP 1 Score: 105.145 bits (261), Expect = 7.734e-25
Identity = 74/196 (37.76%), Postives = 103/196 (52.55%), Query Frame = 1
Query:  385 PGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGAASDFLR---RVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVT-DRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTCSKDFKSFND 960
            PG  K K V FAD  GL L  ++     D P  IP +          S   ++E       +F+QP A  D+L+   R+   +V LE+   +   + GT++V NISFEK V +RITFN W++H D    YV+D++ G   D FSF   LP +   S +RI+FAI +E +   T WDNN G+NY    S   KS N+
Sbjct:   61 PGRAK-KHVSFADHKGLALTVVKIFSEFDDPIDIPDNIDNFFT----SSLTVSEGKDKLTLDFDQPSA--DYLKFRQRIENDHVCLEHCMLKEKSIMGTVKVKNISFEKSVKLRITFNTWKNHTDVECQYVKDTYTGSNRDTFSFEASLPEQV-PSHERIEFAICYEVN-GDTLWDNNQGKNYRIIQSALRKSSNE 247          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Zebrafish
Match: ppp1r3cb (protein phosphatase 1, regulatory subunit 3Cb [Source:NCBI gene;Acc:393807])

HSP 1 Score: 103.99 bits (258), Expect = 2.894e-24
Identity = 72/190 (37.89%), Postives = 101/190 (53.16%), Query Frame = 1
Query:  385 PGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLD-----SDSDTAI----NEKGLNYRANFEQPGAAS-DFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGV-TDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNY 921
            P T   K V FAD+ G+ L  + HVF             +DL+LD     SD + AI     EK  N+   F QP A   DF  R++ + V LEN   +   ++GT++V N+SFEK V VRITF+ W+SH D    Y+ + +     D FSFS  LP+      Q ++F IS++ +   T+WDNN G+NY
Sbjct:   78 PETKAKKKVVFADSKGMSLTAV-HVFK---------EFEEDLMLDLQFELSDLEDAIVGLKAEKEKNFSLGFPQPAADYLDFRNRLKKNLVCLENCIIQERSVTGTVKVSNVSFEKVVHVRITFDSWKSHTDIPCTYMNNVYGCEDVDTFSFSIDLPSFVAPHEQ-VEFCISYK-THDMTYWDNNDGKNY 255          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Xenopus
Match: PPP1R3D (protein phosphatase 1 regulatory subunit 3D [Source:HGNC Symbol;Acc:HGNC:9294])

HSP 1 Score: 107.071 bits (266), Expect = 2.702e-25
Identity = 68/213 (31.92%), Postives = 114/213 (53.52%), Query Frame = 1
Query:  313 TNTDSEFKYYKPKRASSLKTN----RTPPGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAIN-EKGLNY-RANFEQPGAASDFLRRVRMHNVALEN-AFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYT 930
            T  D E +    +R  SL ++    R     PK   VRFAD+ GL+L +++ VF+    P IP   +  L ++SD   +   +  + Y   +F+QP    DFL R++   V LE  A  +   +SGT+ V+N++FEK + VR +F +W+++ +  A + + + +   D F+F+  +P   Q     IQFAI ++ +    +WDNN G+NY +T
Sbjct:   70 TGCDPELRPIMRQRTKSLPSSPERKRAIKCQPKCHKVRFADSLGLELAEVK-VFSSADDPSIPLHVLSRLSINSDLCCSQELQVSIQYLEPDFQQPKDRGDFLDRLQQECVCLERVASSDEKGISGTVWVVNLAFEKDLSVRYSFTNWKNYYEAKAVWQKSAEESSWDVFTFTIPIPPFLQHICSVIQFAIRYQVA-GKEYWDNNQGKNYTFT 280          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Xenopus
Match: nbl1 (NBL1, DAN family BMP antagonist [Source:Xenbase;Acc:XB-GENE-479181])

HSP 1 Score: 110.923 bits (276), Expect = 3.686e-25
Identity = 69/202 (34.16%), Postives = 110/202 (54.46%), Query Frame = 1
Query:  346 PKRASSLKTNR--TPPGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGAASDFLRRVRMHNVALENAFFE--NNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTCSK 939
            P+R SS+ ++     P T  ++ V FADAFG DL  ++     ++P       +   +L  + +T   E+     A+F  P +A D + +V+   V LE+       + + G IRV+N+S+EK+  VR++ +DW+++ D TA Y+  S +G TD+F+F+  L    Q  G  +QF I +E +   TFWDNN GQNY   C K
Sbjct:   42 PRRRSSVSSDEGDLEPPTTVARKVSFADAFGFDLVSVKEFDTWEVP-------IVSQLLSYEVETNYIEE-FYLTASFTLP-SADDIIGKVQAKKVLLESIDMTPGTSSMKGIIRVLNVSYEKQTYVRMSLDDWQTYYDLTAEYIPYSFNGDTDQFAFTITLVPPYQKEGASVQFCICYE-TPIGTFWDNNDGQNYTLICHK 233          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Xenopus
Match: PPP1R3E (protein phosphatase 1 regulatory subunit 3E [Source:NCBI gene;Acc:100486852])

HSP 1 Score: 98.2117 bits (243), Expect = 2.576e-22
Identity = 65/195 (33.33%), Postives = 99/195 (50.77%), Query Frame = 1
Query:  403 KAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDL-------VLDSDSD----------TAINEKGLNYRANFEQPGAASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANY-VQDSHDGV----TDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNY 921
            K VRFADA GL+LE +RH    D+P  IP      L       +L  +S           + +++ G+  +  +E+    + +  +     V L++   E+ FL G++RV+++++EKKV VR T + W+SH D  A Y  +  H G     TD F+F   LP   Q     +QFAI +E  +   FWDNN G+NY
Sbjct:   85 KRVRFADALGLELESVRHFKREDIP-CIPIHVTHRLQGEARRCLLREESSWCEARHGIPASWVSQAGMWEKKTWER----NSWNEQTWQQRVCLQSMRCESCFLWGSVRVLDLAYEKKVTVRYTLDGWQSHQDTYALYAARLCHGGPGHPGTDLFTFRLPLPTRDQQQASCLQFAIRYEVGEEE-FWDNNGGKNY 273          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Xenopus
Match: ppp1r3b (Xenopus tropicalis protein phosphatase 1 regulatory subunit 3B (ppp1r3b), mRNA. [Source:RefSeq mRNA;Acc:NM_001015950])

HSP 1 Score: 98.2117 bits (243), Expect = 2.970e-22
Identity = 62/175 (35.43%), Postives = 100/175 (57.14%), Query Frame = 1
Query:  403 KAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGA-ASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGV-TDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNY 921
            K V FAD+ GL L  ++   + D   +IP +  +  ++D+  +    EK   +  +F QP A   DF  R++  +V LEN   ++  L GT++V N++F+K V +R+T + W+++ D+   YV+D++ G   D FSF   LP   Q+S   I+FA+ FEC +   FWD+N G NY
Sbjct:   78 KRVSFADSRGLALTMVKVYSDFDDELEIPFNISE--LIDNIVNLTTVEKE-RFFLDFVQPSADYLDFRNRLQAESVCLENCMLKDKALVGTVKVKNLAFQKSVKIRMTCDSWQTYKDYDCLYVKDTYAGSDKDTFSFDVSLPEGIQSSAS-IEFAVCFEC-EGRIFWDSNKGLNY 247          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Xenopus
Match: ppp1r3b (Xenopus tropicalis protein phosphatase 1 regulatory subunit 3B (ppp1r3b), mRNA. [Source:RefSeq mRNA;Acc:NM_001015950])

HSP 1 Score: 97.4413 bits (241), Expect = 6.568e-22
Identity = 62/175 (35.43%), Postives = 100/175 (57.14%), Query Frame = 1
Query:  403 KAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGA-ASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGV-TDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNY 921
            K V FAD+ GL L  ++   + D   +IP +  +  ++D+  +    EK   +  +F QP A   DF  R++  +V LEN   ++  L GT++V N++F+K V +R+T + W+++ D+   YV+D++ G   D FSF   LP   Q+S   I+FA+ FEC +   FWD+N G NY
Sbjct:   89 KRVSFADSRGLALTMVKVYSDFDDELEIPFNISE--LIDNIVNLTTVEKE-RFFLDFVQPSADYLDFRNRLQAESVCLENCMLKDKALVGTVKVKNLAFQKSVKIRMTCDSWQTYKDYDCLYVKDTYAGSDKDTFSFDVSLPEGIQSSAS-IEFAVCFEC-EGRIFWDSNKGLNY 258          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Mouse
Match: Ppp1r3b (protein phosphatase 1, regulatory subunit 3B [Source:MGI Symbol;Acc:MGI:2177268])

HSP 1 Score: 107.457 bits (267), Expect = 1.009e-25
Identity = 66/178 (37.08%), Postives = 103/178 (57.87%), Query Frame = 1
Query:  403 KAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGA-ASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGV-TDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYT 930
            K V FAD  GL L  ++     D P  IP +  + L       TA +E   ++  +F QP A   DF  R++ ++V LEN   ++  ++GT++V N++FEK V +R+TF+ W+S  DF   YV+D++ G   D FSF   LP + Q S +R++FA+ +EC+    +WD+N G+NY  T
Sbjct:   60 KRVSFADNQGLALTMVKVFSEFDDPLDIPFNITELLDNIVSLTTAESE---SFVLDFPQPSADYLDFRNRLQTNHVCLENCVLKDKAIAGTVKVQNLAFEKVVKIRMTFDTWKSFTDFPCQYVKDTYAGSDRDTFSFDISLPEKIQ-SYERMEFAVCYECN-GQAYWDSNKGKNYRIT 232          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Mouse
Match: Ppp1r3b (protein phosphatase 1, regulatory subunit 3B [Source:MGI Symbol;Acc:MGI:2177268])

HSP 1 Score: 107.457 bits (267), Expect = 1.009e-25
Identity = 66/178 (37.08%), Postives = 103/178 (57.87%), Query Frame = 1
Query:  403 KAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGA-ASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGV-TDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYT 930
            K V FAD  GL L  ++     D P  IP +  + L       TA +E   ++  +F QP A   DF  R++ ++V LEN   ++  ++GT++V N++FEK V +R+TF+ W+S  DF   YV+D++ G   D FSF   LP + Q S +R++FA+ +EC+    +WD+N G+NY  T
Sbjct:   60 KRVSFADNQGLALTMVKVFSEFDDPLDIPFNITELLDNIVSLTTAESE---SFVLDFPQPSADYLDFRNRLQTNHVCLENCVLKDKAIAGTVKVQNLAFEKVVKIRMTFDTWKSFTDFPCQYVKDTYAGSDRDTFSFDISLPEKIQ-SYERMEFAVCYECN-GQAYWDSNKGKNYRIT 232          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Mouse
Match: Ppp1r3b (protein phosphatase 1, regulatory subunit 3B [Source:MGI Symbol;Acc:MGI:2177268])

HSP 1 Score: 107.457 bits (267), Expect = 1.009e-25
Identity = 66/178 (37.08%), Postives = 103/178 (57.87%), Query Frame = 1
Query:  403 KAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGA-ASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGV-TDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYT 930
            K V FAD  GL L  ++     D P  IP +  + L       TA +E   ++  +F QP A   DF  R++ ++V LEN   ++  ++GT++V N++FEK V +R+TF+ W+S  DF   YV+D++ G   D FSF   LP + Q S +R++FA+ +EC+    +WD+N G+NY  T
Sbjct:   60 KRVSFADNQGLALTMVKVFSEFDDPLDIPFNITELLDNIVSLTTAESE---SFVLDFPQPSADYLDFRNRLQTNHVCLENCVLKDKAIAGTVKVQNLAFEKVVKIRMTFDTWKSFTDFPCQYVKDTYAGSDRDTFSFDISLPEKIQ-SYERMEFAVCYECN-GQAYWDSNKGKNYRIT 232          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Mouse
Match: Ppp1r3a (protein phosphatase 1, regulatory subunit 3A [Source:MGI Symbol;Acc:MGI:2153588])

HSP 1 Score: 110.153 bits (274), Expect = 5.083e-25
Identity = 68/208 (32.69%), Postives = 101/208 (48.56%), Query Frame = 1
Query:  391 TPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGAASDFLRRVRMHNVALENA-FFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTCSKDFKSFNDR*TLNGRMKNPVPLKTI 1011
            T  S+ V FAD+ G  L  ++     +LP     S   D  L  D     +         F+ P +    + ++++    LE+A     + + G IRV+NISFEK V VR++ +DW++H D  A YV +S DG TD+FSF   L    Q  G +++F I +E S + TFW NN G NY+  C K             + K P P+K +
Sbjct:   60 TSASRRVSFADSLGFSLVSVKEFDCWELP-----SVSTDFDLSGD---VFHTDEYVLSPLFDLPSSKEKLMEQLQVQKAVLESAEHLPGSSMKGIIRVLNISFEKLVYVRMSLDDWQTHYDILAEYVPNSCDGETDQFSFKISLVPPYQKEGGKVEFCIRYETS-AGTFWSNNNGTNYILVCQK-------------KRKEPEPVKPL 245          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Mouse
Match: Ppp1r3d (protein phosphatase 1, regulatory subunit 3D [Source:MGI Symbol;Acc:MGI:1917664])

HSP 1 Score: 98.2117 bits (243), Expect = 1.788e-22
Identity = 59/179 (32.96%), Postives = 93/179 (51.96%), Query Frame = 1
Query:  409 VRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRA---NFEQPGAASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDS-HDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTC 933
            VRFADA GL+L  ++ VFN    P +P   +  L ++SD   +  +     +    +F  P     F  R+    V LE     +  +SGT+RV N++FEK+V VR TF+ WRS  +  A +   +  +G  D F+F F +P      G ++ FA+ +  +  + +WDNN G++Y  TC
Sbjct:   83 VRFADALGLELAQVK-VFNAGDDPSVPLHVLSRLAINSDLCCSSQDLEFTLQCLVPDFSPPIETPGFGERLARQLVCLERVTCSDLGISGTVRVRNVAFEKQVTVRYTFSGWRSAHEAVARWRGPAGAEGTEDIFAFGFPVPPFLLELGSQVHFALRYGVA-GAEYWDNNDGRDYSLTC 259          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. UniProt/SwissProt
Match: sp|Q9VVY3|GBS76_DROME (Glycogen-binding subunit 76A OS=Drosophila melanogaster OX=7227 GN=Gbs-76A PE=1 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 9.064e-42
Identity = 89/201 (44.28%), Postives = 122/201 (60.70%), Query Frame = 1
Query:  349 KRASSLKTNRTPPGTP-KSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDL-VLDSDSDTAINEKGLNYRAN-FEQPGAASDFLRRVRMHNVALENAFFENNF---LSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTC 933
            +R SSLKT +TPPGTP + K VRFAD  GLDL D++    LD  P IP SA +DL +L+S+    +  K        F+QPG    FL  VR   V+LENA   +N    +SG++RV N+ F K V +R + + WRS+ D  ANYV++S DG +D   F+F+L       GQR++FA+ F+C +   FWDNN+G NY + C
Sbjct:  438 RRCSSLKTGKTPPGTPGRKKIVRFADVLGLDLADVKTF--LDEIPTIPKSAFEDLEILESEPPLQLGPKSDKLLMPLFQQPGGLPKFLDAVREKQVSLENAAVTDNINQTISGSVRVRNLDFHKSVHIRYSLDGWRSYADLQANYVENSCDGFSD--IFTFVLFGNSLHVGQRLEFAVRFQC-KGQQFWDNNYGANYCFQC 633          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. UniProt/SwissProt
Match: sp|A6QNP3|PPR3B_BOVIN (Protein phosphatase 1 regulatory subunit 3B OS=Bos taurus OX=9913 GN=PPP1R3B PE=2 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 7.536e-26
Identity = 67/175 (38.29%), Postives = 102/175 (58.29%), Query Frame = 1
Query:  403 KAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGA-ASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGV-TDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNY 921
            K V FAD  GL L  ++     D P  IP +  +  +LDS       E   ++  +F QP A   DF  R++  +V LEN   ++  ++GT++V N++FEK V VR+TF+ W+S  DF   YV+D++ G   D FSF   LP + Q S +R++FA+ +EC+   T+WD+N G+NY
Sbjct:   60 KRVSFADNQGLALTMVKVFSEFDDPLDIPLNITE--LLDSIVSLTTAESE-SFVLDFSQPSADYLDFRNRLQTDHVCLENCVLKDRSIAGTVKVQNLAFEKTVKVRMTFDTWKSFTDFPCWYVKDTYAGSDKDTFSFDISLPEKIQ-SYERMEFAVCYECN-GQTYWDSNKGKNY 229          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. UniProt/SwissProt
Match: sp|Q86XI6|PPR3B_HUMAN (Protein phosphatase 1 regulatory subunit 3B OS=Homo sapiens OX=9606 GN=PPP1R3B PE=1 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 1.580e-25
Identity = 65/175 (37.14%), Postives = 103/175 (58.86%), Query Frame = 1
Query:  403 KAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGA-ASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGV-TDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNY 921
            K V FAD  GL L  ++     D P  +P +  + L       TA +E   ++  +F QP A   DF  R++  +V LEN   ++  ++GT++V N++FEK V +R+TF+ W+S+ DF   YV+D++ G   D FSF   LP + Q S +R++FA+ +EC+   T+WD+N G+NY
Sbjct:   61 KRVSFADNQGLALTMVKVFSEFDDPLDMPFNITELLDNIVSLTTAESE---SFVLDFSQPSADYLDFRNRLQADHVCLENCVLKDKAIAGTVKVQNLAFEKTVKIRMTFDTWKSYTDFPCQYVKDTYAGSDRDTFSFDISLPEKIQ-SYERMEFAVYYECN-GQTYWDSNRGKNY 230          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. UniProt/SwissProt
Match: sp|Q6IN01|PPR3B_RAT (Protein phosphatase 1 regulatory subunit 3B OS=Rattus norvegicus OX=10116 GN=Ppp1r3b PE=1 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 4.808e-25
Identity = 67/178 (37.64%), Postives = 103/178 (57.87%), Query Frame = 1
Query:  403 KAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGA-ASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGV-TDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYT 930
            K V FAD  GL L  ++     D P  IP +  + L       TA +E   ++  +F QP A   DF  R++ ++V LEN   +   ++GT++V N++FEK V +R+TF+ W+S  DF   YV+D++ G   D FSF   LP + Q S +R++FA+ +EC+  S +WD+N G+NY  T
Sbjct:   60 KRVSFADNQGLALTMVKVFSEFDDPLDIPFNITELLDNIVSLTTAESE---SFVLDFPQPSADYLDFRNRLQTNHVCLENCVLKEKAIAGTVKVQNLAFEKVVKIRMTFDTWKSFTDFPCQYVKDTYAGSDRDTFSFDISLPEKIQ-SYERMEFAVCYECNGQS-YWDSNKGKNYRIT 232          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. UniProt/SwissProt
Match: sp|O95685|PPR3D_HUMAN (Protein phosphatase 1 regulatory subunit 3D OS=Homo sapiens OX=9606 GN=PPP1R3D PE=1 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 4.828e-25
Identity = 77/258 (29.84%), Postives = 122/258 (47.29%), Query Frame = 1
Query:  217 ISPVDSVASSNGSECRWIGNSKFHQEKSSDSETNTDSEFKYYKPKRASSL--------KTNRTP-----PGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYR-----ANFEQPGAASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDS-HDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTC 933
            +S +D   +     CR  G S       + + +  D   +    +RA SL        K    P     PG  +   VRFADA GL+L  ++ VFN    P +P   +  L ++SD     + + L +       +F  P  A+DF  R++   V LE     +  +SGT+RV N++FEK+V VR TF+ WRS  +  A +   +  +G  D F+F F +P      G R+ FA+ ++ +  + +WDNN  ++Y  TC
Sbjct:   27 LSDLDGGVALEPRACRPPG-SPGRAPPPTPAPSGCDPRLRPIILRRARSLPSSPERRQKAAGAPGAACRPGCSQKLRVRFADALGLELAQVK-VFNAGDDPSVPLHVLSRLAINSD--LCCSSQDLEFTLHCLVPDFPPPVEAADFGERLQRQLVCLERVTCSDLGISGTVRVCNVAFEKQVAVRYTFSGWRSTHEAVARWRGPAGPEGTEDVFTFGFPVPPFLLELGSRVHFAVRYQVA-GAEYWDNNDHRDYSLTC 279          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. TrEMBL
Match: A0A3S4ZMZ2 (CBM21 domain-containing protein OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS886 PE=4 SV=1)

HSP 1 Score: 235.728 bits (600), Expect = 3.055e-71
Identity = 116/207 (56.04%), Postives = 150/207 (72.46%), Query Frame = 1
Query:  322 DSEFKYYKPKRASSLKTNRTPPGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSD-SDTAINEKGLNYRANFEQPGAASDFLRRVRMHNVALENAFFEN--NFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTC 933
            D+E++YYKPKR+SSLK+ RTPP TP  KAVRFAD FGLDL  ++ VF+ D PPKIPASA+QDL LDSD S ++I  K  +YR  F QPGA+  F+ RV    V+LE+A  +   N L+GT+RV +  FEK + +RITFN+W++H + +A YVQ SHDG+TDRFSFS + P  C  +G++ QFAI +E      FWDNN+GQNYV TC
Sbjct:   18 DNEYQYYKPKRSSSLKSARTPPDTPARKAVRFADVFGLDLTTVKTVFDHDAPPKIPASAMQDLRLDSDLSLSSIGAK--HYRLCFAQPGASPSFMTRVLAQTVSLESADIDQTRNMLTGTVRVKSFGFEKHIFIRITFNNWQTHSETSAAYVQGSHDGMTDRFSFSIVFPV-CMVAGEQAQFAIRYETHTGLLFWDNNYGQNYVVTC 221          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. TrEMBL
Match: A0A5J4NTF7 (Protein phosphatase 1 regulatory subunit 3A/B/C/D/E OS=Paragonimus westermani OX=34504 GN=DEA37_0012964 PE=4 SV=1)

HSP 1 Score: 222.631 bits (566), Expect = 6.673e-62
Identity = 116/205 (56.59%), Postives = 139/205 (67.80%), Query Frame = 1
Query:  328 EFKYYKPKRASSLKTNRTPPGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTA-INEKGLNYRANFEQPGAASDFLRRVRMHNVALENAFFE--NNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTC 933
            E +  KPKR+SSLK+ RTPPGTP  KAVRFADAFGLDLE +RHVF+L+ PPKIPASA  DL LD+D   A I  K   +   F QPG AS+F+RRV    V+LE+A  +     L+GTIRV +  FEK+V VRIT+N+W ++ D  A YVQ SHDG TDRFSFS + P +    G R QFAI +E      FWDNN GQNY  TC
Sbjct:  411 ETEMPKPKRSSSLKSQRTPPGTPGQKAVRFADAFGLDLESVRHVFDLEAPPKIPASATFDLHLDTDESIARIGAK--QFGLCFPQPGLASNFVRRVLNQAVSLEDARVDMPRGLLTGTIRVKSFGFEKRVHVRITYNNWMTYCDTPAAYVQGSHDGATDRFSFSVVFP-DTMIPGDRAQFAIRYETHTGEQFWDNNNGQNYSVTC 612          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. TrEMBL
Match: H2KRZ3 (CBM21 domain-containing protein CG9619 OS=Clonorchis sinensis OX=79923 GN=CLF_107618 PE=4 SV=1)

HSP 1 Score: 221.861 bits (564), Expect = 1.453e-61
Identity = 111/199 (55.78%), Postives = 136/199 (68.34%), Query Frame = 1
Query:  343 KPKRASSLKTNRTPPGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGAASDFLRRVRMHNVALENAFFE--NNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTC 933
            KPKR+SSLK+ RTPPGTP  K VRFADA GLDLE +RHVF+ + PPKIPASA  DL LD+D   A       +R  F QPG+A++F+RRV    V LE+A  E     L+GTIRV ++ FEK+V VR T+NDW ++ D TA+YVQ SHDG TDRFSFS + P +    G+R QFAI ++      FWDNNFGQNY   C
Sbjct:  411 KPKRSSSLKSQRTPPGTPGQKVVRFADALGLDLETVRHVFDQENPPKIPASATFDLELDTDESIA-KLGAKQFRMCFSQPGSAANFIRRVLDQTVCLEDAHVEMPRGVLNGTIRVRSLGFEKRVFVRATYNDWSTYCDTTASYVQGSHDGATDRFSFSLVFP-DTMVPGRRAQFAIRYDAHTGEQFWDNNFGQNYSVMC 607          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. TrEMBL
Match: A0A075A050 (Putative phosphatase regulatory subunit OS=Opisthorchis viverrini OX=6198 GN=T265_00986 PE=4 SV=1)

HSP 1 Score: 221.476 bits (563), Expect = 1.844e-61
Identity = 111/199 (55.78%), Postives = 136/199 (68.34%), Query Frame = 1
Query:  343 KPKRASSLKTNRTPPGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGAASDFLRRVRMHNVALENAFFE--NNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTC 933
            KPKR+SSLK+ RTPPGTP  K VRFADA GLDLE +RHVF+ + PPKIPASA  DL LD+D   A       +R  F QPG+A++F+RRV    V LE+A  E     L+GTIRV ++ FEK+V VR T+NDW ++ D TA+YVQ SHDG TDRFSFS + P +    G+R QFAI ++      FWDNNFGQNY   C
Sbjct:  411 KPKRSSSLKSQRTPPGTPGQKVVRFADALGLDLETVRHVFDQENPPKIPASATFDLELDTDESIA-KLGAKQFRMCFSQPGSAANFIRRVLDQTVCLEDAHVEMPRGVLNGTIRVRSLGFEKRVFVRATYNDWSTYCDTTASYVQGSHDGATDRFSFSLVFP-DTMVPGRRAQFAIRYDAHTGEQFWDNNFGQNYSVMC 607          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. TrEMBL
Match: A0A4S2M3R6 (CBM21 domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_002908 PE=4 SV=1)

HSP 1 Score: 220.705 bits (561), Expect = 3.399e-61
Identity = 110/199 (55.28%), Postives = 136/199 (68.34%), Query Frame = 1
Query:  343 KPKRASSLKTNRTPPGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGAASDFLRRVRMHNVALENAFFE--NNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTC 933
            KPKR+SSLK+ RTPPGTP  K VRFADA GLDLE +RHVF+ + PPKIPASA  DL +D+D   A       +R  F QPG+A++F+RRV    V LE+A  E     L+GTIRV ++ FEK+V VR T+NDW ++ D TA+YVQ SHDG TDRFSFS + P +    G+R QFAI ++      FWDNNFGQNY   C
Sbjct:  411 KPKRSSSLKSQRTPPGTPGQKVVRFADALGLDLESVRHVFDQENPPKIPASATFDLEIDTDESIA-KLGAKQFRMCFSQPGSAANFIRRVLDQTVCLEDAHVEMPRGVLNGTIRVRSLGFEKRVFVRATYNDWSTYCDTTASYVQGSHDGATDRFSFSLVFP-DTMVPGRRAQFAIRYDAHTGEQFWDNNFGQNYSVMC 607          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Cavefish
Match: ENSAMXT00000026535.2 (pep primary_assembly:Astyanax_mexicanus-2.0:18:5639658:5645568:1 gene:ENSAMXG00000025809.2 transcript:ENSAMXT00000026535.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 112.464 bits (280), Expect = 2.163e-27
Identity = 63/179 (35.20%), Postives = 98/179 (54.75%), Query Frame = 1
Query:  403 KAVRFADAFGLDLEDIRHVFNLDLPPKIPASA------VQDLVLDSDSDTAINEKGLNYRANFEQPGAASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNY 921
            K VRFAD+ GL+L  ++H  + D  P+IP          + + L++      + + +     F  P A+  F++RVR   V+LE    +   LSG +RV+N++FEK V +R T N+W + +D  A+YV +S DG+TD+F F  + P   +  G  +QFA+ +       FWDNN G NY
Sbjct:  115 KKVRFADSLGLELISVKHFDDAD-EPEIPERVTDRFRKARAMHLNTLGVPGASAQSVFMEVLFSNPCASPSFMQRVRDLRVSLERVDADEFCLSGLVRVLNMAFEKSVTLRYTINNWATFVDAPASYVPESSDGITDKFCFKIVTPMFLEAGGT-LQFAVKYRV-DGQEFWDNNGGSNY 290          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Cavefish
Match: ppp1r3b (protein phosphatase 1 regulatory subunit 3B [Source:NCBI gene;Acc:103028921])

HSP 1 Score: 102.834 bits (255), Expect = 4.673e-24
Identity = 85/243 (34.98%), Postives = 117/243 (48.15%), Query Frame = 1
Query:  271 GNSKFHQEKSSDSETNTDSEFKYYKPKRASSLKTNRTPP-------------GTPKS---------KAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGAASDFLR-RVRMHN--VALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGV-TDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNY 921
             N +F   KSS          KY +P R   L  NR PP             G  +S         K V FAD  GL L  ++     D P  +P S +Q L     S   ++E+      +F QP A  D+LR R R+    V LEN   ++  ++GT++V N+SFEK V +RITF+ W+SH D    YV+D++ G   D FSF   LP +     +RI+FA+ F      T+WD+N GQNY
Sbjct:   12 ANDEFQSRKSS----------KYKRPLRPC-LHPNRVPPLGFRSSPQQPERNGVQESSLKDRGRTKKQVSFADHKGLALTMVKIFSKFDDPIDVPLS-IQQLF---SSALCVSEEENKLVLDFAQPSA--DYLRFRQRLEEDLVCLENCMLKDRAVAGTVKVKNLSFEKFVKLRITFDTWKSHTDVDCLYVKDTYVGSDRDTFSFEVNLPEQVPPH-ERIEFAV-FYVVNGITYWDSNRGQNY 235          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Cavefish
Match: ppp1r3da (protein phosphatase 1 regulatory subunit 3D-like [Source:NCBI gene;Acc:103042623])

HSP 1 Score: 100.138 bits (248), Expect = 6.368e-23
Identity = 67/227 (29.52%), Postives = 114/227 (50.22%), Query Frame = 1
Query:  289 QEKSSDSETNTDSEFKYYKPKRASSLKTNRTPPGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANF------EQPGAASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSH-----DGVT-DRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTC 933
            Q +S+    + +   K    +RA SL +        +   VRF D+ G+DLE+++ VF     P +P   +  L+++++   +   KGL     +      EQPG +++FL R+    V LE        + G ++V+N++FEK+V VR +F +W+S  +  AN+V + +     DG + D F F   +P      G  ++FA+ ++    + FWDNN GQNY   C
Sbjct:   94 QPRSTGRSLSCEPPPKPIIHRRAQSLPSPSEQRRFTRRTGVRFVDSLGMDLENVK-VFKSGEDPFVPEHVLFRLLMNAELAAS---KGLEISLPYLKPVFSEQPGDSANFLERLFRQKVCLERVLCYEPGIIGIVQVLNLAFEKEVYVRYSFTNWKSCSETKANWVGNKYMEGLSDGCSCDSFRFHLPVPPFLLHPGAVLEFAVRYKV-LDAQFWDNNDGQNYKLVC 315          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Cavefish
Match: ppp1r3db (protein phosphatase 1, regulatory subunit 3Db [Source:ZFIN;Acc:ZDB-GENE-110201-1])

HSP 1 Score: 97.0561 bits (240), Expect = 3.148e-22
Identity = 62/189 (32.80%), Postives = 93/189 (49.21%), Query Frame = 1
Query:  397 KSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQP------GAASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSH----DGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTC 933
            +   VRF D+ GL+LE ++  F     P +P   +  L+  + S+ A   K L     + QP       +  DFL+R+   +V LE        + GT++V+N++FEK V VR +F DW+S  +  A +V   H    +  +D F F   +P      G  +QFAI F+ SQ   +WDNN G NY   C
Sbjct:   81 RGSQVRFVDSLGLELERVK-FFKASEDPTVPVHVICRLL--ASSELAFG-KHLELTLPYFQPSFPENMASQPDFLQRLCRQHVCLEQVHCSELGIIGTVQVLNLAFEKDVTVRYSFTDWKSSAESKACWVSTLHRNGDEPESDIFRFRLPVPPFILKPGATLQFAICFQ-SQGVEYWDNNDGHNYKLAC 264          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Cavefish
Match: ppp1r3cb (protein phosphatase 1 regulatory subunit 3C [Source:NCBI gene;Acc:103045860])

HSP 1 Score: 96.6709 bits (239), Expect = 7.358e-22
Identity = 68/183 (37.16%), Postives = 102/183 (55.74%), Query Frame = 1
Query:  409 VRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLD-SDSDTAIN----EKGLNYRANFEQPGAAS-DFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSF------LLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNY 921
            V FAD+ G+ L  I HVF      +    A+ DL  + +D + AI     EK  ++  +F QP A   DF  R+  ++V LEN   ++  L+GT++V N+SFEK V VRITF+ W+S+ D    Y+ + + G TD  +FSF      L+P E     + + F IS++ +   T+WDNN G+NY
Sbjct:   86 VVFADSKGMSLTAI-HVFK-----EFEEDAMSDLQFELADLENAIGGMKVEKEKSFVLDFPQPAADYLDFRNRLIKNSVCLENCVIQDRTLTGTVKVSNVSFEKAVDVRITFDSWKSYTDVPCAYMNNVY-GCTDIDTFSFTIDLPSLVPPE-----KHVDFCISYK-THDQTYWDNNDGKNY 255          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Sea Lamprey
Match: ppp1r3b (protein phosphatase 1, regulatory subunit 3B [Source:NCBI gene;Acc:327285])

HSP 1 Score: 104.375 bits (259), Expect = 6.293e-25
Identity = 70/186 (37.63%), Postives = 100/186 (53.76%), Query Frame = 1
Query:  397 KSKAVRFADAFGLDLEDIRHVFNLDLP-PKIPASAVQDLVLD---SDSDTAINEKGLNYRA-----NFEQPGA-ASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTS-GQRIQFAISFECSQSSTFWDNNFGQNY 921
            K K V FADA GL L  +R    L+ P P++    + +L L+   S     I   G+   +     +F QP A   DF RR+  + V+LEN       LSGTI+V N+ FEK V +RITF+ W+S  D    ++ D++ G +D  +FSF +   C  S  +R++F IS+ CS  +  WDNN G NY
Sbjct:   90 KKKRVVFADARGLALASVRLFSELEDPCPELLQFELSELHLEEVVSKLAPEIKRNGMGGASGRLVLDFTQPSADYLDFRRRLNGNMVSLENCLLNERVLSGTIKVKNLGFEKTVVLRITFDTWKSSTDVECCFLHDAYGG-SDTDTFSFQVETPCHVSPHERVEFCISYTCS-GTEHWDNNGGSNY 273          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Yeast
Match: GIP2 (Putative regulatory subunit of protein phosphatase Glc7p; involved in glycogen metabolism; contains a conserved motif (GVNK motif) that is also found in Gac1p, Pig1p, and Pig2p; GIP2 has a paralog, PIG2, that arose from the whole genome duplication [Source:SGD;Acc:S000000856])

HSP 1 Score: 47.7506 bits (112), Expect = 2.991e-6
Identity = 32/108 (29.63%), Postives = 54/108 (50.00%), Query Frame = 1
Query:  658 FLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGV---TDRFSFSFLLPAECQTSGQ-RIQFAISFECS---QSSTFWDNNFGQNYVYTCSKDFKSFND 960
             ++G I V NI ++K+V VR T++ WR+  +    Y+ D  DG+   T+   F F++    +   + +++F I +      +   +WDNN G NY      D   FND
Sbjct:  438 LIAGRILVKNIFYDKRVVVRYTWDSWRTTHEVECVYISDG-DGILPGTNMDIFHFIIDDVSKVDPRGKLEFCIHYSTRNDYEREEYWDNNNGNNYKVDVVMD--GFND 542          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Nematostella
Match: EDO41629 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S413])

HSP 1 Score: 102.834 bits (255), Expect = 5.135e-26
Identity = 57/124 (45.97%), Postives = 69/124 (55.65%), Query Frame = 1
Query:  571 FEQPGAASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTCSKD 942
            F QP A   F  R+    V +ENA    N + GTI+V NIS+ K+V VR T + W S  D  A+YV DS DG +DRFSF+  +P     SG  I FAI F  +    FWDNN GQNY   C  D
Sbjct:   17 FSQPVALEGFKLRLENEFVCMENASTSRNTIMGTIKVKNISYHKEVLVRYTLDSWHSFTDVPASYVLDSFDGNSDRFSFAVTIPEYFMASGGNIDFAICFR-ADGKEFWDNNSGQNYKVECRDD 139          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Nematostella
Match: EDO41628 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S412])

HSP 1 Score: 98.5969 bits (244), Expect = 6.762e-24
Identity = 48/126 (38.10%), Postives = 71/126 (56.35%), Query Frame = 1
Query:  562 RANFEQPGAASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTCSK 939
            + +FEQP +  DF  R++   V LEN  F    L+GTI V NI F K V VR + ++WR+  D  A+YV     G  DRF F   +    +  G +++F++ F C +S  +WDNN+ +NY+  C K
Sbjct:   76 KCDFEQPSSREDFKSRLKKQKVCLENVAFSGYALTGTILVENIEFCKDVTVRYSMDEWRTFTDVWADYVNQLAGGTLDRFQFRISITQNIEV-GSKLEFSVRF-CVRSKEYWDNNYCRNYIVECKK 199          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Nematostella
Match: EDO47072 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RN47])

HSP 1 Score: 90.5077 bits (223), Expect = 6.100e-22
Identity = 47/122 (38.52%), Postives = 67/122 (54.92%), Query Frame = 1
Query:  577 QPGAASDFLRRVRMHNVALENAFFENN---FLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTC 933
            QP +  D+ +      V+LENAF   N    L G IRV NI++EKKV VR + ++W S++D  A ++  SH+G  D++ F  L P      G  I+FA+ + C      WDNN G NY+  C
Sbjct:    2 QPSSRHDWNKHSIQRKVSLENAFVGGNNGMVLVGLIRVWNIAYEKKVFVRYSTDNWSSYVDLPAEFMSQSHEGTIDKYVFKLLAPVGLAV-GDTIEFAVCY-CVNEQKHWDNNKGNNYIVKC 121          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Nematostella
Match: EDO48422 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJS3])

HSP 1 Score: 90.1225 bits (222), Expect = 9.873e-21
Identity = 59/197 (29.95%), Postives = 96/197 (48.73%), Query Frame = 1
Query:  403 KAVRFADAFGLDLEDIRHVFNLD---------LPPKIPASAVQDLVLDSDSDTAINEKGLN------YRANFEQPGAASDFLRRVRMHNVALENAFF---ENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTCSK 939
            K+V FAD  G  L  ++H+             +  KI +  + ++  ++++       G N       +  F QP ++ DFL R+R   V LENA+     N  L G IRV NIS+EK+V  R + + W    D  A ++  +++G  D+F F  +LP +C   G  ++FA+ F+      +WDNN G N+   C +
Sbjct:   34 KSVTFADVLGEQLVSVKHITPTSSYEDVKKAFIRRKINSRKIYEVKDENNNIIPGRYPGANGLSKTYLKCCFPQPASSPDFLERMRTQMVCLENAYMGDSSNLELIGLIRVRNISYEKEVFARYSIDHWIHFQDCMAAHLLQTNEGKFDKFIFKLVLP-KCFYVGGTVEFAVCFK-KNGQEYWDNNRGNNFTVKCCE 228          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Nematostella
Match: EDO48424 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJS5])

HSP 1 Score: 88.9669 bits (219), Expect = 2.550e-20
Identity = 61/197 (30.96%), Postives = 97/197 (49.24%), Query Frame = 1
Query:  403 KAVRFADAFGLDLEDIRHV--------FNLD-LPPKIPASAVQDLVLDSDS---DTAINEKGLN---YRANFEQPGAASDFLRRVRMHNVALENAFF---ENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTCSK 939
            K+V FAD  G  L  ++H+         N+  +  KI +  + ++  D+++        E GL+    +  F QP ++ DFL R+R   V LENA+     N  L G IRV NIS+EK+V  R + + W    D  A ++  ++ G  D+F F  +LP      G  ++FA+ F+      +WDNN G N+   C +
Sbjct:   34 KSVTFADVLGKQLVSVKHITPTSSYEDVNIAFIRSKICSRKIYEVTDDNNNIIPRRCPGENGLSKTYLKCCFPQPASSPDFLERMRTQMVCLENAYMGDSSNLELIGLIRVRNISYEKEVFARYSIDHWIHFQDCMAAHLLQTNRGEFDKFIFKLVLPKGFYVGGT-VEFAVCFK-KNGQEYWDNNRGNNFTVKCCE 228          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Medaka
Match: ENSORLT00000011971.2 (protein phosphatase 1 regulatory subunit 3E [Source:NCBI gene;Acc:101160970])

HSP 1 Score: 110.153 bits (274), Expect = 5.174e-27
Identity = 67/179 (37.43%), Postives = 96/179 (53.63%), Query Frame = 1
Query:  403 KAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDL------VLDSDSDTAINEKGLNYRANFEQPGAASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNY 921
            K VRFAD+ GL+L  ++H FN    P++P   +  L       L +    A ++  L     F  PG    F ++VR   V LE+   +   LSG +RV+N++FEK V +R + N+W + +D  A YV +S DGVTD+F F  ++P      G  +QFAI + C     FWDNN G NY
Sbjct:   62 KKVRFADSLGLELISVKH-FNDADDPEVPERILAKLHKGNLHWLYTKFPRAPSQPVL-MELQFTNPGTLPGFEQKVREVKVLLESVEADEFSLSGFVRVLNMAFEKSVSLRYSLNNWITFMDSLATYVPNSSDGVTDKFCFKIVMPTYLDNGGA-LQFAIKY-CVGGREFWDNNHGNNY 236          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Medaka
Match: ppp1r3db (protein phosphatase 1, regulatory subunit 3Db [Source:ZFIN;Acc:ZDB-GENE-110201-1])

HSP 1 Score: 105.145 bits (261), Expect = 3.041e-25
Identity = 70/214 (32.71%), Postives = 108/214 (50.47%), Query Frame = 1
Query:  322 DSEFKYYKPKRASSLKTNRTPPGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTA-INEKGLNY-RANF-EQPGAASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDG-------VTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTC 933
            D+  K    +RA SL          +   VRF DA GLDLE+I+ VF +   P +P   +  L+++S+     + E  L Y +A F E   A  +FL  +    V LE     ++ ++GTIRV+N+++EK+V V  +F +WR+  D TA +V   H+G        TD F F   +P      G  ++FA+ +  +    +WDNN G NY  +C
Sbjct:   29 DTTAKTSMRRRAQSLPGGSVRRTELRGARVRFIDALGLDLEEIK-VFRVQEHPLVPEHVIFRLLMNSERAFGKLPELNLPYFKACFPENMAAQPEFLPNLVSRKVCLEQLTCSDHGITGTIRVLNLAYEKEVKVLYSFTNWRTRTDTTACWVMRGHEGDSSISALDTDLFRFHLPVPPFILQPGAVLEFAVRYHVT-GHDYWDNNNGSNYKLSC 240          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Medaka
Match: ENSORLT00000013158.2 (protein phosphatase 1 regulatory subunit 3A [Source:NCBI gene;Acc:105357035])

HSP 1 Score: 101.679 bits (252), Expect = 2.743e-22
Identity = 63/186 (33.87%), Postives = 94/186 (50.54%), Query Frame = 1
Query:  382 PPGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGAASDFLRRVRMHNVALENAFF--ENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTC 933
            PP   + K V FADAFGLDL  ++   N +      A+  +D   +      ++E+ L     F  P    +   R++   + LE+         L G IRV+N+ + K + VRIT + W SH D  A+Y+  S D  TDRF+FS+ L    +  G R++F + +E + + TFW NN G NYV  C
Sbjct:   43 PPPVVRRK-VSFADAFGLDLVSVKEFDNAE------AAEAEDCWPEGGDGGRLSEE-LYLTCLFTVPLPPEELDLRLQTQMLELESIELLPGTTTLRGIIRVVNLCYAKNIYVRITLDQWSSHFDMPADYIPGSSDMRTDRFTFSYTLTPPFEKEGSRVEFCLRYE-TPAGTFWANNKGMNYVLFC 219          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Medaka
Match: ppp1r3b (protein phosphatase 1, regulatory subunit 3B [Source:NCBI gene;Acc:327285])

HSP 1 Score: 95.5153 bits (236), Expect = 6.290e-22
Identity = 62/197 (31.47%), Postives = 99/197 (50.25%), Query Frame = 1
Query:  343 KPKRASSLKTNRTPPGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGAASDFL---RRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGV-TDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNY 921
            +P RA          G    K V FAD  GL L  ++       P +IP +  + L          N   L+    F QP  +SD+L   + +  + V+LE+   +    +GTI+V N+S+EK V +R+TF+ W+SH+D    YV+D++    +D FS+   LPAE     + ++FA+ +E    +  WD+N G+NY
Sbjct:   18 RPDRAEQTGPEAGWTGAKAKKRVTFADHRGLTLTSVKVFSEFTDPIQIPVNIQEMLRCAPPPSPEENRLVLD----FTQP--SSDYLLFRQNLETNQVSLEHCVLKEKAFAGTIKVKNVSYEKSVTLRVTFDSWKSHLDVPCRYVKDAYPSPHSDTFSYQVALPAELPPH-EHVEFAVCYEVG-GAQHWDSNQGRNY 206          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Medaka
Match: ENSORLT00000028909.1 (protein phosphatase 1 regulatory subunit 3A [Source:NCBI gene;Acc:105354194])

HSP 1 Score: 98.9821 bits (245), Expect = 1.820e-21
Identity = 68/208 (32.69%), Postives = 100/208 (48.08%), Query Frame = 1
Query:  346 PKRASSLKTNRTPPGTP--KSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRAN---FEQPGAASDFLRRVRMHNVALENAFF--ENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTCSKDFK 948
            P+R SS+      P  P   S+ V FADA GL L +++  F+     K+P         D+   TAI+  G  Y  +   F    ++ +   +V    + LE   F      L G IRV+NIS+ K V +R T + W +H D  A Y+  S DGVTD FSF   +    +  G R++F + +E  +  TFW NN  +NYV  C +  +
Sbjct:   44 PRRKSSITDEDLEPDVPLCGSRRVSFADAKGLSLVEVKE-FDTWEVSKLPGQ-------DASGATAID--GEEYYLSPLTFSLTLSSEEVFAKVLEQKIELETIDFLPGTTILKGVIRVLNISYSKAVFIRTTLDQWATHFDLLAEYIPGSSDGVTDCFSFRLTVVPWFREQGARVEFCLRYETPE-GTFWANNNARNYVLLCHRRIR 240          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Planmine SMEST
Match: SMESG000033999.1 (SMESG000033999.1)

HSP 1 Score: 624.009 bits (1608), Expect = 0.000e+0
Identity = 300/301 (99.67%), Postives = 300/301 (99.67%), Query Frame = 1
Query:   61 MSEFATEIIFCQKNSIYYPTLGEIVQKASMASTYSSISTCDSGIELWNLSKTISPVDSVASSNGSECRWIGNSKFHQEKSSDSETNTDSEFKYYKPKRASSLKTNRTPPGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGAASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTCSKDFKSFNDR 963
            MSEFATEIIFCQKNSIYYPTLGEIVQKASMASTYSSISTCDSGIELWNLSKTISPVDSVASSNGSECRWI NSKFHQEKSSDSETNTDSEFKYYKPKRASSLKTNRTPPGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGAASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTCSKDFKSFNDR
Sbjct:    1 MSEFATEIIFCQKNSIYYPTLGEIVQKASMASTYSSISTCDSGIELWNLSKTISPVDSVASSNGSECRWISNSKFHQEKSSDSETNTDSEFKYYKPKRASSLKTNRTPPGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGAASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTCSKDFKSFNDR 301          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Planmine SMEST
Match: SMESG000018209.1 (SMESG000018209.1)

HSP 1 Score: 624.009 bits (1608), Expect = 0.000e+0
Identity = 300/301 (99.67%), Postives = 300/301 (99.67%), Query Frame = 1
Query:   61 MSEFATEIIFCQKNSIYYPTLGEIVQKASMASTYSSISTCDSGIELWNLSKTISPVDSVASSNGSECRWIGNSKFHQEKSSDSETNTDSEFKYYKPKRASSLKTNRTPPGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGAASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTCSKDFKSFNDR 963
            MSEFATEIIFCQKNSIYYPTLGEIVQKASMASTYSSISTCDSGIELWNLSKTISPVDSVASSNGSECRWI NSKFHQEKSSDSETNTDSEFKYYKPKRASSLKTNRTPPGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGAASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTCSKDFKSFNDR
Sbjct:    1 MSEFATEIIFCQKNSIYYPTLGEIVQKASMASTYSSISTCDSGIELWNLSKTISPVDSVASSNGSECRWISNSKFHQEKSSDSETNTDSEFKYYKPKRASSLKTNRTPPGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGAASDFLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTCSKDFKSFNDR 301          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Planmine SMEST
Match: SMESG000061419.1 (SMESG000061419.1)

HSP 1 Score: 127.102 bits (318), Expect = 8.260e-33
Identity = 78/208 (37.50%), Postives = 114/208 (54.81%), Query Frame = 1
Query:  325 SEFKYYKPKRASSLKTNRTPPGTPKSKAVRFADAFGLDLEDIRHVFNLDL--PPKIPASAVQDLVLDSDSDTAINEKGLNYRANFEQPGAASD---FLRRVRMHNVALENAFFENNFLSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTC 933
            S+  +    R S  + NR      KS  VRFADA GL L +I+ + N D+   P + +SA++DL LD +   A+  +G+ Y      P    D    +     +N+ LE A+ +   L G I V NIS+EK VG+RIT+N+W S  D  A+Y+ +S +G+ D+FSFS ++P      G +I+FA+ +      TFWDNN  QNYV  C
Sbjct:   97 SDIDFSNDNRRSKSQVNRKTDKCRKS--VRFADALGLQLSEIKFIKNSDIEKAPVLTSSAIKDLHLDHNCHLAM--QGIKYFC----PCFKIDEKTVIENTMKNNIQLEKAYIQGMTLFGRIFVKNISYEKNVGIRITYNNWVSFKDIYASYILESSNGIIDKFSFSVVVPRSV-IVGSKIEFAVFYLTQNRETFWDNNSNQNYVLEC 295          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Planmine SMEST
Match: SMESG000061009.1 (SMESG000061009.1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.512e-14
Identity = 65/197 (32.99%), Postives = 95/197 (48.22%), Query Frame = 1
Query:  400 SKAVRFADAFGLDLEDIRH---VFNLDLPP--KIPASAVQDLVLDSDSDTAINEKGLNYRA---NFEQPGAASDFL---RRVRMHNVALENAFF----ENNF-LSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVT-------DRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNY 921
            SK V FAD  G +L  IR    + NL+     +I  S + +  +   +    ++  L ++     F  P  A D     +R+    VALE+       +N + LSGTI+V N+ FEK V VR+TFN+W S  D+ A Y   S+   T       D F F   LP   +     ++F+I F C ++  FWDNN G+NY
Sbjct:    4 SKKVTFADEKGFNLFTIRKEEKLENLNFKQLYEIERSIINNKTISESTVNQTDKLPLAFQKWIIKFSDP--ALDLFQVEKRLTYQKVALESVRIFSKSDNKWQLSGTIKVKNMDFEKLVFVRVTFNNWNSFKDYKAIYCFTSNFSYTLETSENNDIFWFEIPLPQVLKNETLSVEFSIKFIC-KNYEFWDNNKGENY 197          
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Planmine SMEST
Match: SMESG000061009.1 (SMESG000061009.1)

HSP 1 Score: 72.4034 bits (176), Expect = 2.597e-14
Identity = 68/209 (32.54%), Postives = 99/209 (47.37%), Query Frame = 1
Query:  373 NRTPPGTPK---SKAVRFADAFGLDLEDIRH---VFNLDLPP--KIPASAVQDLVLDSDSDTAINEKGLNYRA---NFEQPGAASDFL---RRVRMHNVALENAFF----ENNF-LSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVT-------DRFSFSFLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNY 921
            N  P  + K   SK V FAD  G +L  IR    + NL+     +I  S + +  +   +    ++  L ++     F  P  A D     +R+    VALE+       +N + LSGTI+V N+ FEK V VR+TFN+W S  D+ A Y   S+   T       D F F   LP   +     ++F+I F C ++  FWDNN G+NY
Sbjct:   12 NFVPLKSKKMVISKKVTFADEKGFNLFTIRKEEKLENLNFKQLYEIERSIINNKTISESTVNQTDKLPLAFQKWIIKFSDP--ALDLFQVEKRLTYQKVALESVRIFSKSDNKWQLSGTIKVKNMDFEKLVFVRVTFNNWNSFKDYKAIYCFTSNFSYTLETSENNDIFWFEIPLPQVLKNETLSVEFSIKFIC-KNYEFWDNNKGENY 217          
The following BLAST results are available for this feature:
BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PPP1R3B3.290e-2637.14protein phosphatase 1 regulatory subunit 3B [Sourc... [more]
PPP1R3B3.290e-2637.14protein phosphatase 1 regulatory subunit 3B [Sourc... [more]
PPP1R3B3.290e-2637.14protein phosphatase 1 regulatory subunit 3B [Sourc... [more]
PPP1R3B3.290e-2637.14protein phosphatase 1 regulatory subunit 3B [Sourc... [more]
PPP1R3D1.005e-2529.84protein phosphatase 1 regulatory subunit 3D [Sourc... [more]
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BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
H18N23.21.082e-1934.76Protein phosphatase 1 regulatory subunit [Source:... [more]
H18N23.21.168e-1934.76Protein phosphatase 1 regulatory subunit [Source:... [more]
H18N23.22.670e-1934.76Protein phosphatase 1 regulatory subunit [Source:... [more]
CC8.21.589e-925.53Protein phosphatase 1 regulatory subunit [Source:... [more]
CC8.22.187e-925.53Protein phosphatase 1 regulatory subunit [Source:... [more]
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BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Gbs-76A9.042e-4344.28gene:FBgn0036862 transcript:FBtr0331425[more]
Gbs-76A9.042e-4344.28gene:FBgn0036862 transcript:FBtr0075014[more]
Gbs-76A9.042e-4344.28gene:FBgn0036862 transcript:FBtr0345390[more]
Gbs-76A9.042e-4344.28gene:FBgn0036862 transcript:FBtr0332155[more]
Gbs-70E4.821e-1733.33gene:FBgn0036428 transcript:FBtr0075723[more]
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BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ppp1r3b7.734e-2537.76protein phosphatase 1, regulatory subunit 3B [Sour... [more]
ppp1r3b7.734e-2537.76protein phosphatase 1, regulatory subunit 3B [Sour... [more]
ppp1r3b7.734e-2537.76protein phosphatase 1, regulatory subunit 3B [Sour... [more]
ppp1r3b7.734e-2537.76protein phosphatase 1, regulatory subunit 3B [Sour... [more]
ppp1r3cb2.894e-2437.89protein phosphatase 1, regulatory subunit 3Cb [Sou... [more]
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BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PPP1R3D2.702e-2531.92protein phosphatase 1 regulatory subunit 3D [Sourc... [more]
nbl13.686e-2534.16NBL1, DAN family BMP antagonist [Source:Xenbase;Ac... [more]
PPP1R3E2.576e-2233.33protein phosphatase 1 regulatory subunit 3E [Sourc... [more]
ppp1r3b2.970e-2235.43Xenopus tropicalis protein phosphatase 1 regulator... [more]
ppp1r3b6.568e-2235.43Xenopus tropicalis protein phosphatase 1 regulator... [more]
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BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ppp1r3b1.009e-2537.08protein phosphatase 1, regulatory subunit 3B [Sour... [more]
Ppp1r3b1.009e-2537.08protein phosphatase 1, regulatory subunit 3B [Sour... [more]
Ppp1r3b1.009e-2537.08protein phosphatase 1, regulatory subunit 3B [Sour... [more]
Ppp1r3a5.083e-2532.69protein phosphatase 1, regulatory subunit 3A [Sour... [more]
Ppp1r3d1.788e-2232.96protein phosphatase 1, regulatory subunit 3D [Sour... [more]
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BLAST of Protein phosphatase 1 regulatory subunit 3A vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9VVY3|GBS76_DROME9.064e-4244.28Glycogen-binding subunit 76A OS=Drosophila melanog... [more]
sp|A6QNP3|PPR3B_BOVIN7.536e-2638.29Protein phosphatase 1 regulatory subunit 3B OS=Bos... [more]
sp|Q86XI6|PPR3B_HUMAN1.580e-2537.14Protein phosphatase 1 regulatory subunit 3B OS=Hom... [more]
sp|Q6IN01|PPR3B_RAT4.808e-2537.64Protein phosphatase 1 regulatory subunit 3B OS=Rat... [more]
sp|O95685|PPR3D_HUMAN4.828e-2529.84Protein phosphatase 1 regulatory subunit 3D OS=Hom... [more]
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BLAST of Protein phosphatase 1 regulatory subunit 3A vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3S4ZMZ23.055e-7156.04CBM21 domain-containing protein OS=Protopolystoma ... [more]
A0A5J4NTF76.673e-6256.59Protein phosphatase 1 regulatory subunit 3A/B/C/D/... [more]
H2KRZ31.453e-6155.78CBM21 domain-containing protein CG9619 OS=Clonorch... [more]
A0A075A0501.844e-6155.78Putative phosphatase regulatory subunit OS=Opistho... [more]
A0A4S2M3R63.399e-6155.28CBM21 domain-containing protein OS=Opisthorchis fe... [more]
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BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000026535.22.163e-2735.20pep primary_assembly:Astyanax_mexicanus-2.0:18:563... [more]
ppp1r3b4.673e-2434.98protein phosphatase 1 regulatory subunit 3B [Sourc... [more]
ppp1r3da6.368e-2329.52protein phosphatase 1 regulatory subunit 3D-like [... [more]
ppp1r3db3.148e-2232.80protein phosphatase 1, regulatory subunit 3Db [Sou... [more]
ppp1r3cb7.358e-2237.16protein phosphatase 1 regulatory subunit 3C [Sourc... [more]
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BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ppp1r3b6.293e-2537.63protein phosphatase 1, regulatory subunit 3B [Sour... [more]
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BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
GIP22.991e-629.63Putative regulatory subunit of protein phosphatase... [more]
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BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO416295.135e-2645.97Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO416286.762e-2438.10Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO470726.100e-2238.52Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO484229.873e-2129.95Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO484242.550e-2030.96Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000011971.25.174e-2737.43protein phosphatase 1 regulatory subunit 3E [Sourc... [more]
ppp1r3db3.041e-2532.71protein phosphatase 1, regulatory subunit 3Db [Sou... [more]
ENSORLT00000013158.22.743e-2233.87protein phosphatase 1 regulatory subunit 3A [Sourc... [more]
ppp1r3b6.290e-2231.47protein phosphatase 1, regulatory subunit 3B [Sour... [more]
ENSORLT00000028909.11.820e-2132.69protein phosphatase 1 regulatory subunit 3A [Sourc... [more]
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BLAST of Protein phosphatase 1 regulatory subunit 3A vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000033999.10.000e+099.67SMESG000033999.1[more]
SMESG000018209.10.000e+099.67SMESG000018209.1[more]
SMESG000061419.18.260e-3337.50SMESG000061419.1[more]
SMESG000061009.11.512e-1432.99SMESG000061009.1[more]
SMESG000061009.12.597e-1432.54SMESG000061009.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024231 ID=SMED30024231|Name=Protein phosphatase 1 regulatory subunit 3A|organism=Schmidtea mediterranea sexual|type=transcript|length=1439bp
CTTGGTTAAATAAGGTAATTATGCTGAATTTATTTTTATTTATTTTTCAA
ATTTTAAAAAATGTCAGAGTTTGCTACTGAGATAATTTTTTGTCAAAAAA
ATTCAATTTATTATCCAACTCTTGGAGAAATTGTTCAAAAAGCATCTATG
GCTTCAACATATTCATCAATATCAACATGTGACTCTGGAATCGAATTGTG
GAATCTTTCCAAAACCATTTCTCCAGTAGACAGTGTAGCATCATCAAATG
GTTCAGAGTGTCGATGGATCGGTAATTCAAAATTTCACCAAGAGAAGAGT
AGTGATTCTGAAACAAATACAGATTCTGAATTCAAATACTACAAACCAAA
AAGAGCTAGCTCGCTGAAAACAAATCGTACTCCTCCTGGAACACCTAAAT
CTAAAGCAGTTCGTTTTGCAGACGCGTTTGGACTAGATTTAGAAGACATT
AGACATGTTTTTAATCTTGATTTGCCTCCAAAAATACCAGCATCAGCAGT
TCAAGATTTGGTGTTAGACAGTGATTCAGATACCGCCATCAATGAAAAAG
GTTTGAATTACAGAGCTAATTTCGAACAGCCGGGTGCCGCTAGTGATTTT
CTTCGACGTGTGAGAATGCACAATGTCGCTCTGGAAAACGCTTTTTTCGA
AAACAATTTTTTATCGGGCACAATTCGAGTAATGAATATCAGTTTTGAAA
AAAAGGTCGGTGTACGAATAACGTTTAATGATTGGAGATCGCATATAGAC
TTTACAGCCAATTATGTTCAAGATTCTCATGACGGTGTTACGGATCGGTT
TTCGTTTTCATTTTTATTACCAGCTGAATGTCAGACTTCTGGACAACGAA
TTCAGTTTGCTATATCATTCGAATGTTCACAATCATCAACGTTTTGGGAC
AATAATTTTGGTCAAAATTATGTGTACACATGCTCCAAAGATTTCAAAAG
TTTTAATGATCGATAAACTTTAAATGGCAGAATGAAAAATCCAGTGCCTT
TGAAAACGATATATTGTGAGATATTCTTTTCGAATTTCTTTGAAGTGACT
TTCGATAATTGAGCATATTATATTGTTAGGATTCACTAGACATTCAATAT
TTCTTGGTGACAGTGTTAGATTTACCGTATTTTTCTAGTTCCATTTATTC
AATTTTTGTACATAACATATTTTGGCAATAGTCCCAAATCCGTGATATAT
TTTCATTTTGGTGGTGCATAATGATCCGTAGTTTGTCATTTTAGTCAAAC
TTGTTTTAATTGTTGATTTGTGATTATAAGAATAAAATCTTAATGTTGTT
GCAATTATATTGGCATCATGAGATTATAAAAGCTGTACCTCATCGCAATT
TGGATAAAAATAAAGTTCAATTTTATTTTAATGTGTTTAATTAAAAATTT
ATGTGATTAAAATAGCGTATGTGTTTTTCCAGAAACATA
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protein sequence of SMED30024231-orf-1

>SMED30024231-orf-1 ID=SMED30024231-orf-1|Name=SMED30024231-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=302bp
MSEFATEIIFCQKNSIYYPTLGEIVQKASMASTYSSISTCDSGIELWNLS
KTISPVDSVASSNGSECRWIGNSKFHQEKSSDSETNTDSEFKYYKPKRAS
SLKTNRTPPGTPKSKAVRFADAFGLDLEDIRHVFNLDLPPKIPASAVQDL
VLDSDSDTAINEKGLNYRANFEQPGAASDFLRRVRMHNVALENAFFENNF
LSGTIRVMNISFEKKVGVRITFNDWRSHIDFTANYVQDSHDGVTDRFSFS
FLLPAECQTSGQRIQFAISFECSQSSTFWDNNFGQNYVYTCSKDFKSFND
R*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0000164protein phosphatase type 1 complex
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000418head
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR038175CBM21_dom_sf
IPR005036CBM21_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
Vocabulary: biological process
TermDefinition
GO:0005975carbohydrate metabolic process
GO:0005977glycogen metabolic process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005036CBM21 (carbohydrate binding type-21) domainPFAMPF03370CBM_21coord: 185..288
e-value: 7.0E-28
score: 97.1
IPR005036CBM21 (carbohydrate binding type-21) domainPROSITEPS51159CBM21coord: 181..290
score: 22.688
IPR038175CBM21 domain superfamilyGENE3DG3DSA:2.60.40.2440coord: 159..294
e-value: 3.1E-39
score: 135.9
NoneNo IPR availablePANTHERPTHR12307PROTEIN PHOSPHATASE 1 BINDING PROTEINcoord: 72..293
NoneNo IPR availablePANTHERPTHR12307:SF44GLYCOGEN-BINDING SUBUNIT 76Acoord: 72..293