SMED30024217

Overview
NameSMED30024217
Smed IDSMED30024217
Length (bp)1402
Neoblast Clusters

Zeng et. al., 2018



 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30024217

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
germ cellSMED30024217SMESG000065539.1 Contig43317newmark_estsPMID:27612384
Roberts-Galbraith et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
germ cellSMED30024217SMESG000065539.1 Contig43317uc_Smed_v2PMID:27612384
Roberts-Galbraith et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
reproductive organSMED30024217SMESG000065539.1 Contig27635newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30024217SMESG000065539.1 Contig27635uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30024217SMESG000065539.1 Contig43317newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30024217SMESG000065539.1 Contig43317uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
testisSMED30024217SMESG000065539.1 PL08009B1E11_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
early male germ cellSMED30024217SMESG000065539.1 PL08009B1E11_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism asexual adult cDNA to DNA expression microarray evidence
testis primordiumSMED30024217SMESG000065539.1 PL08009B1E11_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism juvenile cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30024217 vs. Ensembl Celegans
Match: ppk-1 (PIP Kinase [Source:UniProtKB/TrEMBL;Acc:O01759])

HSP 1 Score: 52.7582 bits (125), Expect = 4.281e-7
Identity = 43/199 (21.61%), Postives = 86/199 (43.22%), Query Frame = 2 
Query:  119 LIFNTQYGKYSLHKIKDSEMCIITSYLKRYYQCSMGMNYSFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSST---------KSSSYNGLNDFKY--NHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDEIKNNRRTSVLPK----MYKSSDAENHNVYR 670
            + + +Q  ++ +  ++  E   +   L  YY        + +PKF G++ Y+ + GKN++    L+     P    +H++    YD+K ST         ++  +  L D  +  NH DG  ++    + +++    D   +   +++ YSL + +HN  L  +E     + NR +   P      +    AENH V +
Sbjct:  185 IFYVSQDDQFIIKTVQHKEADFLQKLLPGYYMNLNQNPRTLLPKFFGLFCYQ-SLGKNIR---LLVMNNLLPQTVTMHEK----YDMKGSTYKRMANKAERAKPHPTLKDLDFLENHKDGIFIDPTALEALMKSISRDCLVMESFKIMDYSLLVGIHNVELGIKE---RAEQNRESHPAPSTSSDTFSGEKAENHKVLQ 372
[Close]
BLAST of SMED30024217 vs. Ensembl Fly
Match: PIP5K59B (gene:FBgn0034789 transcript:FBtr0290095)

HSP 1 Score: 60.077 bits (144), Expect = 2.929e-9
Identity = 43/171 (25.15%), Postives = 77/171 (45.03%), Query Frame = 2 
Query:  152 LHKIKDSEMCIITSYLKRYYQCSMGMNY---SFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSST------------KSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDEIKNNRRTSV 619
            +  ++  E   +   L  YY   M +N    + +PKF G+Y  + ++ KN++    ++     PS  ++H    L YD+K ST            KS +Y  L DF   HP+G  +       +I+  + D   L   +++ YSL + VHN +++ +E   E +   R  +
Sbjct:  188 IKTVQHKEGEFLQKLLPGYY---MNLNQNPRTLLPKFFGLYCLQTSNAKNIR---LVVMNNLLPSSVKMH----LKYDLKGSTFKRKANKAERAKKSPTYKDL-DFMEQHPNGIFLEAETYAALIKTIQRDCTVLESFKIMDYSLLLGVHNLDVALKEKQSEQRKPLRAPL 347
[Close]
BLAST of SMED30024217 vs. Ensembl Fly
Match: PIP5K59B (gene:FBgn0034789 transcript:FBtr0071900)

HSP 1 Score: 60.077 bits (144), Expect = 2.996e-9
Identity = 42/156 (26.92%), Postives = 72/156 (46.15%), Query Frame = 2 
Query:  197 LKRYYQCSMGMNY---SFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSST------------KSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDEIKNNRRTSV 619
            L  YY   M +N    + +PKF G+Y  + ++ KN++    ++     PS  ++H    L YD+K ST            KS +Y  L DF   HP+G  +       +I+  + D   L   +++ YSL + VHN +++ +E   E +   R  +
Sbjct:  203 LPGYY---MNLNQNPRTLLPKFFGLYCLQTSNAKNIR---LVVMNNLLPSSVKMH----LKYDLKGSTFKRKANKAERAKKSPTYKDL-DFMEQHPNGIFLEAETYAALIKTIQRDCTVLESFKIMDYSLLLGVHNLDVALKEKQSEQRKPLRAPL 347
[Close]
BLAST of SMED30024217 vs. Ensembl Fly
Match: PIP5K59B (gene:FBgn0034789 transcript:FBtr0304923)

HSP 1 Score: 60.077 bits (144), Expect = 3.178e-9
Identity = 42/156 (26.92%), Postives = 72/156 (46.15%), Query Frame = 2 
Query:  197 LKRYYQCSMGMNY---SFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSST------------KSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDEIKNNRRTSV 619
            L  YY   M +N    + +PKF G+Y  + ++ KN++    ++     PS  ++H    L YD+K ST            KS +Y  L DF   HP+G  +       +I+  + D   L   +++ YSL + VHN +++ +E   E +   R  +
Sbjct:  203 LPGYY---MNLNQNPRTLLPKFFGLYCLQTSNAKNIR---LVVMNNLLPSSVKMH----LKYDLKGSTFKRKANKAERAKKSPTYKDL-DFMEQHPNGIFLEAETYAALIKTIQRDCTVLESFKIMDYSLLLGVHNLDVALKEKQSEQRKPLRAPL 347
[Close]
BLAST of SMED30024217 vs. Ensembl Fly
Match: PIP5K59B (gene:FBgn0034789 transcript:FBtr0304921)

HSP 1 Score: 59.6918 bits (143), Expect = 3.598e-9
Identity = 42/156 (26.92%), Postives = 72/156 (46.15%), Query Frame = 2 
Query:  197 LKRYYQCSMGMNY---SFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSST------------KSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDEIKNNRRTSV 619
            L  YY   M +N    + +PKF G+Y  + ++ KN++    ++     PS  ++H    L YD+K ST            KS +Y  L DF   HP+G  +       +I+  + D   L   +++ YSL + VHN +++ +E   E +   R  +
Sbjct:  203 LPGYY---MNLNQNPRTLLPKFFGLYCLQTSNAKNIR---LVVMNNLLPSSVKMH----LKYDLKGSTFKRKANKAERAKKSPTYKDL-DFMEQHPNGIFLEAETYAALIKTIQRDCTVLESFKIMDYSLLLGVHNLDVALKEKQSEQRKPLRAPL 347
[Close]
BLAST of SMED30024217 vs. Ensembl Fly
Match: PIP5K59B (gene:FBgn0034789 transcript:FBtr0339762)

HSP 1 Score: 59.6918 bits (143), Expect = 3.799e-9
Identity = 43/171 (25.15%), Postives = 77/171 (45.03%), Query Frame = 2 
Query:  152 LHKIKDSEMCIITSYLKRYYQCSMGMNY---SFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSST------------KSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDEIKNNRRTSV 619
            +  ++  E   +   L  YY   M +N    + +PKF G+Y  + ++ KN++    ++     PS  ++H    L YD+K ST            KS +Y  L DF   HP+G  +       +I+  + D   L   +++ YSL + VHN +++ +E   E +   R  +
Sbjct:  188 IKTVQHKEGEFLQKLLPGYY---MNLNQNPRTLLPKFFGLYCLQTSNAKNIR---LVVMNNLLPSSVKMH----LKYDLKGSTFKRKANKAERAKKSPTYKDL-DFMEQHPNGIFLEAETYAALIKTIQRDCTVLESFKIMDYSLLLGVHNLDVALKEKQSEQRKPLRAPL 347
[Close]
BLAST of SMED30024217 vs. Ensembl Zebrafish
Match: pip5k1cb (phosphatidylinositol-4-phosphate 5-kinase, type I, gamma b [Source:ZFIN;Acc:ZDB-GENE-110408-21])

HSP 1 Score: 53.5286 bits (127), Expect = 4.650e-7
Identity = 41/164 (25.00%), Postives = 77/164 (46.95%), Query Frame = 2 
Query:  119 LIFNTQYGKYSLHKIKDSEMCIITSYLKRYYQCSMGMNY---SFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFEL---FYDVKSSTKSSSYNGLN----DFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRE 580
            + + T+  ++ L  +   E   +   L  YY   M +N    + +PKF G+Y  + + GKN++    ++     P  FR+H +F+L    Y  ++S K    N       DF  +  DG L+++     +++  + D   L   +++ YSL + VHN   ++RE
Sbjct:  174 IFYVTRDDEFILKTVMHKEAEFLQKLLPGYY---MNLNQNPRTLLPKFFGLYCVQ-SGGKNIR---IVVMNNVLPRVFRMHLKFDLKGSTYKRRASKKEREKNRPTYKDLDFMQDVQDGLLLDVDTYNALLKTLQRDCLVLESFKIMDYSLLLGVHNVEQAERE 330
[Close]
BLAST of SMED30024217 vs. Ensembl Zebrafish
Match: pip5k1cb (phosphatidylinositol-4-phosphate 5-kinase, type I, gamma b [Source:ZFIN;Acc:ZDB-GENE-110408-21])

HSP 1 Score: 53.1434 bits (126), Expect = 5.367e-7
Identity = 41/164 (25.00%), Postives = 77/164 (46.95%), Query Frame = 2 
Query:  119 LIFNTQYGKYSLHKIKDSEMCIITSYLKRYYQCSMGMNY---SFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFEL---FYDVKSSTKSSSYNGLN----DFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRE 580
            + + T+  ++ L  +   E   +   L  YY   M +N    + +PKF G+Y  + + GKN++    ++     P  FR+H +F+L    Y  ++S K    N       DF  +  DG L+++     +++  + D   L   +++ YSL + VHN   ++RE
Sbjct:  174 IFYVTRDDEFILKTVMHKEAEFLQKLLPGYY---MNLNQNPRTLLPKFFGLYCVQ-SGGKNIR---IVVMNNVLPRVFRMHLKFDLKGSTYKRRASKKEREKNRPTYKDLDFMQDVQDGLLLDVDTYNALLKTLQRDCLVLESFKIMDYSLLLGVHNVEQAERE 330
[Close]
BLAST of SMED30024217 vs. Ensembl Xenopus
Match: pip5k1a (phosphatidylinositol-4-phosphate 5-kinase, type I, alpha [Source:NCBI gene;Acc:448746])

HSP 1 Score: 54.6842 bits (130), Expect = 1.839e-7
Identity = 47/197 (23.86%), Postives = 88/197 (44.67%), Query Frame = 2 
Query:  143 KYSLHKIKDSEMCIITSYLKRYYQCSMGMNY---SFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSST------------KSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRE--LDDEIKNN-RRTSVLPKMYKSSDAENHNVYRRAS 679
            ++ +  ++  E   +   L  YY   M +N    + +PKF G+Y  +   GKN++    ++     P   R+H    L YD+K ST            KS +Y  L DF  + PDG  ++      + +  + D   L+  +++ YSL + VHN + +++E  +D   +++ RR +    +Y ++        RR  
Sbjct:   28 EFIIKTVQHKEAEFLQKLLPGYY---MNLNQNPRTLLPKFYGLYCVQAG-GKNIR---IVVMNNLLPRSVRMH----LKYDLKGSTYKRRASAKEREKKSPTYKDL-DFLQDLPDGLFLDPDMHNALCKTIQRDCLVLQSFKIMDYSLLVGVHNMDCAQKETAVDGHTQSDTRRPAAQKALYSTAMESIQGEARRGG 212
[Close]
BLAST of SMED30024217 vs. Ensembl Mouse
Match: Pip5k1c (phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma [Source:MGI Symbol;Acc:MGI:1298224])

HSP 1 Score: 49.2914 bits (116), Expect = 9.401e-6
Identity = 45/177 (25.42%), Postives = 81/177 (45.76%), Query Frame = 2 
Query:  143 KYSLHKIKDSEMCIITSYLKRYYQCSMGMNY---SFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFEL---FYDVKSSTKS-----SSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDEIKNNRRTSVLPKMYKS 640
            K  +HK    E   +   L  YY   M +N    + +PKF G+Y  + + GKN++    ++  V  P   ++H +F+L    Y  ++S K       +Y  L DF  + P+G L++      +++  + D   L   +++ YSL + VHN +  +RE   E   ++     P   K+
Sbjct:  188 KTVMHK----EAEFLQKLLPGYY---MNLNQNPRTLLPKFYGLYCVQ-SGGKNIRVV--VMNNV-LPRVVKMHLKFDLKGSTYKRRASKKEKEKSLPTYKDL-DFMQDMPEGLLLDSDTFGALVKTLQRDCLVLESFKIMDYSLLLGVHNIDQQERERQAEGAQSKADEKRPVAQKA 352
[Close]
BLAST of SMED30024217 vs. UniProt/SwissProt
Match: sp|O48709|PI5K3_ARATH (Phosphatidylinositol 4-phosphate 5-kinase 3 OS=Arabidopsis thaliana OX=3702 GN=PIP5K3 PE=4 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 7.137e-7
Identity = 52/196 (26.53%), Postives = 84/196 (42.86%), Query Frame = 2 
Query:  131 TQYGKYSLHKIKDSEMCIITSYLKRYYQCSMGMNYSFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSSTKSSSYNGLN--------DFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVH--------NFNLSKRELDDEIKNNRRTSVLPKMYKSSDAENHNVYR 670
            TQ  +Y +  +K SE+ ++   L  YY+       S + KF G++  K   G+    T F++    F SE+RIHK+F+L       T       ++        D KY     F +  +  +  I   + D EFL   R++ YSL I +H        + +L     D E K  R    L ++ +S+ A+   V R
Sbjct:  434 TQDERYMIKTMKKSEIKVLLKMLPNYYEHVSKYKNSLVTKFFGVHCVKPVGGQK---TRFIVMGNLFCSEYRIHKRFDLKGSSHGRTIDKDEGEIDETTTLKDLDLKYV----FRLETSWFQAFINQIDLDCEFLEAERIMDYSLLIGLHFRESGMRDDISLGIGRRDQEDKLMRGNGPLMRLGESTPAKAEQVSR 622
[Close]
BLAST of SMED30024217 vs. TrEMBL
Match: A0A1B0GC28 (PIPK domain-containing protein OS=Glossina morsitans morsitans OX=37546 PE=4 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.954e-8
Identity = 73/319 (22.88%), Postives = 125/319 (39.18%), Query Frame = 2 
Query:  236 SFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSST------------KSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDEIKNNRRTSVLPKMYKSSDAENHNVYRRASISPYRTPLDDNQRMKIGEKHPPKVNHHHSSSSDTASLIIIGSNRESKGRTSDLAAIMAKGFNKVNTTFLFNSAKLQCDFYKAKRHGAIFVYDVKGEHLAVFLSFGHIFDKTEDLYTYKSLLKSTFKCRC----TRKITTIASYADGFENFLLQQAFRCV 1144
            + +PKF G+Y  + ++GKN++    ++     PS  ++H    L YD+K ST            KS +Y  L DF  +HP+G  +       +I+  + D   L   +++ YSL + VHN +L+ +E     K  ++T      Y++ D++N       +                      K N  +S +++  +   I  NR ++ R    +  M              +     D       G I     KGE L +F+    I       Y  K  L+ TFK       T  +   + YA  F+NF+ +  FR +
Sbjct:  107 TLLPKFFGLYCLQSSNGKNIR---LVVMNNLLPSYVKMH----LKYDLKGSTFKRKASKAERAKKSPTYKDL-DFMEHHPNGIFLEAETYSALIKTIQRDCTVLESFKIMDYSLLVGVHNLDLATKEKQKIQKPPQKT-----QYQTEDSDNEETTPTVA----------------------KANETNSDNNEDDTQSNINRNRSNRQRLVAHSTAMES----------IQAESEPIDDEDDMPPGGIPARSEKGERLLLFVGIIDILQS----YRLKKKLEHTFKSIIHDGDTVSVCRPSFYAQRFQNFMAKTVFRKI 376
[Close]
BLAST of SMED30024217 vs. TrEMBL
Match: A0A5K3ESS6 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 2.872e-8
Identity = 81/355 (22.82%), Postives = 136/355 (38.31%), Query Frame = 2 
Query:  143 KYSLHKIKDSEMCIITSYLKRYYQCSMGMNYSFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSSTKSSSYNGLN-----------DFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDEIKNNRRTSVLPKMYKSSDAENHNVYRRASISPYRTPLDDNQRMKIGEKHPPKVNHHHSSSSDTASLIIIGSNRESKGRTSDLAAIMAKGFNKVNTTFLFNSAKLQ------CDFYKAKRHGAIFVYDVKGEHLAVFLSFGHIFDKTEDLYTYKSLLKSTFKCRCTRKITTIASYADGFENFLLQQAFRCVKPDM 1156
            ++ +  ++  E   +   L  YY   +    + +PKF G++ Y+ + GKN++   F++     PS  ++H++    YD+K S+     N              DFK  HPDG  +       +++  E D   L   R++ YSL + VHNF+ ++R+ + + + +    V+P+   SS   +H    RAS                    PP           TAS   +     S  RT     +  K     +T      AK++       D  KA   G +      G+ L +F+    I          +  LKS      T  +T  + YA  F+N L    FR +   M
Sbjct:  169 EFIIKTVQHKEANFLQKLLPEYYMNLVQHTRTLLPKFYGLHCYQCS-GKNIR---FVVMNNLLPSSVKMHER----YDLKGSSYKRKANDRELAKASPTLKDLDFKERHPDGIWLEADTYDALMKTIERDCRVLESFRIMDYSLLLGVHNFDQAERDRNRKKERSSDNGVIPEDGLSS---SHAPNLRAS-------------------SPPVAAGRKGGDVITASRRSVSPFNRSAFRTK----LGNKRLTAFSTAMESIEAKVEPVEIEPTDPGKATLLGGLPARSASGDRLFLFIGIIDILQSYRMAKKMEHTLKSVVIDAETVSVTNPSFYARRFQNALGTCIFRKIPSPM 489
[Close]
BLAST of SMED30024217 vs. TrEMBL
Match: G4LZK4 (Phosphatidylinositol-4-phosphate 5-kinase type I, putative OS=Schistosoma mansoni OX=6183 GN=Smp_124970 PE=4 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 3.173e-8
Identity = 64/253 (25.30%), Postives = 116/253 (45.85%), Query Frame = 2 
Query:  119 LIFNTQYGKYSLHKIKDSEMCIITSYLKRYYQCSMGMNYSFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSST-----------KSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRE-LDDEIKNNRRTSVLPKMYKSSDAENHNVYRRAS---ISPYRTPLDDNQRMKIGEKHPPKVNHHHSSSSDTASLIIIGSNRESKGRTS 832
            + + +Q  ++ +  ++  E   +   L  Y+   M    + +PK+ G+Y Y+ +  KN++   F++     PS  RIH++    YD+K ST           KSS      DF  NHPDG  +       +++  E D   L+  R++ YSL + +HN + +KRE L  +  + ++T+V  +  +SSD   +++ +  S   IS      ++N             N   S S+   S  ++G+  ES  +TS
Sbjct:  159 IFYISQDDEFIIKTVQHKEGEFLQKLLPEYFMNLMQHPRTLLPKYYGLYCYQ-SGHKNIR---FVVMNNLLPSSVRIHEK----YDLKGSTYGRQASDAERAKSSPTLKDLDFIENHPDGIWLEAETYDALLKTIERDCLVLKSFRIMDYSLLLGIHNLDQAKRERLARKQASEKQTTVNQRGDESSDLSPNSIPKMKSNGLISHISNKSNENN-----------TNQSSSPSNGVGSGGLLGTVNESSNKTS 392
[Close]
BLAST of SMED30024217 vs. TrEMBL
Match: A0A5K4F7F6 (Phosphatidylinositol-4-phosphate 5-kinase type I, putative OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 3.211e-8
Identity = 64/253 (25.30%), Postives = 116/253 (45.85%), Query Frame = 2 
Query:  119 LIFNTQYGKYSLHKIKDSEMCIITSYLKRYYQCSMGMNYSFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSST-----------KSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRE-LDDEIKNNRRTSVLPKMYKSSDAENHNVYRRAS---ISPYRTPLDDNQRMKIGEKHPPKVNHHHSSSSDTASLIIIGSNRESKGRTS 832
            + + +Q  ++ +  ++  E   +   L  Y+   M    + +PK+ G+Y Y+ +  KN++   F++     PS  RIH++    YD+K ST           KSS      DF  NHPDG  +       +++  E D   L+  R++ YSL + +HN + +KRE L  +  + ++T+V  +  +SSD   +++ +  S   IS      ++N             N   S S+   S  ++G+  ES  +TS
Sbjct:  159 IFYISQDDEFIIKTVQHKEGEFLQKLLPEYFMNLMQHPRTLLPKYYGLYCYQ-SGHKNIR---FVVMNNLLPSSVRIHEK----YDLKGSTYGRQASDAERAKSSPTLKDLDFIENHPDGIWLEAETYDALLKTIERDCLVLKSFRIMDYSLLLGIHNLDQAKRERLARKQASEKQTTVNQRGDESSDLSPNSIPKMKSNGLISHISNKSNENN-----------TNQSSSPSNGVGSGGLLGTVNESSNKTS 392
[Close]
BLAST of SMED30024217 vs. TrEMBL
Match: A0A5K3ESR6 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 3.330e-8
Identity = 50/193 (25.91%), Postives = 92/193 (47.67%), Query Frame = 2 
Query:  143 KYSLHKIKDSEMCIITSYLKRYYQCSMGMNYSFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSST-----------KSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDEIKNNRRTSVLPKMYKSSDAENHNVYRRASISP 688
            ++ +  ++  E   +   L  YY   +    + +PKF G++ Y+ + GKN++   F++     PS  ++H++    YD+K S+           K+S      DFK  HPDG  +       +++  E D   L   R++ YSL + VHNF+ ++R+ + + + +    V+P+   SS   +H    RAS  P
Sbjct:  169 EFIIKTVQHKEANFLQKLLPEYYMNLVQHTRTLLPKFYGLHCYQCS-GKNIR---FVVMNNLLPSSVKMHER----YDLKGSSYKRKANDRELAKASPTLKDLDFKERHPDGIWLEADTYDALMKTIERDCRVLESFRIMDYSLLLGVHNFDQAERDRNRKKERSSDNGVIPEDGLSS---SHAPNLRASSPP 350
[Close]
BLAST of SMED30024217 vs. Ensembl Cavefish
Match: pip5k1cb (phosphatidylinositol 4-phosphate 5-kinase type-1 gamma-like [Source:NCBI gene;Acc:103026952])

HSP 1 Score: 55.8398 bits (133), Expect = 6.201e-8
Identity = 43/169 (25.44%), Postives = 81/169 (47.93%), Query Frame = 2 
Query:  119 LIFNTQYGKYSLHKIKDSEMCIITSYLKRYYQCSMGMNY---SFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFEL---FYDVKSSTKS-----SSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDE 592
            + + T+  ++ +  ++  E   +   L  YY   M +N    + +PKF G+Y  + + GKN++    L+     P  FR+H +F+L    Y  ++S K       ++  L DF    PDG L++      +++  + D   L   +++ YSL + VHN + ++RE   E
Sbjct:  174 IFYVTRDDEFIIKTVQHKEAEFLQKLLPGYY---MNLNQNPRTLLPKFFGLYCIQ-SGGKNIR---ILVMNNVLPRVFRMHLKFDLKGSTYKRRASKKEREKSRPTFKDL-DFLQEVPDGLLLDTDTFSALVKTLQRDCLVLESFKIMDYSLLLGVHNLDQAEREQQTE 334
[Close]
BLAST of SMED30024217 vs. Ensembl Cavefish
Match: pip5k1cb (phosphatidylinositol 4-phosphate 5-kinase type-1 gamma-like [Source:NCBI gene;Acc:103026952])

HSP 1 Score: 55.8398 bits (133), Expect = 6.510e-8
Identity = 43/169 (25.44%), Postives = 81/169 (47.93%), Query Frame = 2 
Query:  119 LIFNTQYGKYSLHKIKDSEMCIITSYLKRYYQCSMGMNY---SFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFEL---FYDVKSSTKS-----SSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDE 592
            + + T+  ++ +  ++  E   +   L  YY   M +N    + +PKF G+Y  + + GKN++    L+     P  FR+H +F+L    Y  ++S K       ++  L DF    PDG L++      +++  + D   L   +++ YSL + VHN + ++RE   E
Sbjct:  174 IFYVTRDDEFIIKTVQHKEAEFLQKLLPGYY---MNLNQNPRTLLPKFFGLYCIQ-SGGKNIR---ILVMNNVLPRVFRMHLKFDLKGSTYKRRASKKEREKSRPTFKDL-DFLQEVPDGLLLDTDTFSALVKTLQRDCLVLESFKIMDYSLLLGVHNLDQAEREQQTE 334
[Close]
BLAST of SMED30024217 vs. Ensembl Cavefish
Match: pip5k1cb (phosphatidylinositol 4-phosphate 5-kinase type-1 gamma-like [Source:NCBI gene;Acc:103026952])

HSP 1 Score: 55.4546 bits (132), Expect = 7.497e-8
Identity = 43/169 (25.44%), Postives = 81/169 (47.93%), Query Frame = 2 
Query:  119 LIFNTQYGKYSLHKIKDSEMCIITSYLKRYYQCSMGMNY---SFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFEL---FYDVKSSTKS-----SSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDE 592
            + + T+  ++ +  ++  E   +   L  YY   M +N    + +PKF G+Y  + + GKN++    L+     P  FR+H +F+L    Y  ++S K       ++  L DF    PDG L++      +++  + D   L   +++ YSL + VHN + ++RE   E
Sbjct:  159 IFYVTRDDEFIIKTVQHKEAEFLQKLLPGYY---MNLNQNPRTLLPKFFGLYCIQ-SGGKNIR---ILVMNNVLPRVFRMHLKFDLKGSTYKRRASKKEREKSRPTFKDL-DFLQEVPDGLLLDTDTFSALVKTLQRDCLVLESFKIMDYSLLLGVHNLDQAEREQQTE 319
[Close]
BLAST of SMED30024217 vs. Ensembl Cavefish
Match: pip5k1cb (phosphatidylinositol 4-phosphate 5-kinase type-1 gamma-like [Source:NCBI gene;Acc:103026952])

HSP 1 Score: 55.4546 bits (132), Expect = 8.125e-8
Identity = 43/169 (25.44%), Postives = 81/169 (47.93%), Query Frame = 2 
Query:  119 LIFNTQYGKYSLHKIKDSEMCIITSYLKRYYQCSMGMNY---SFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFEL---FYDVKSSTKS-----SSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDE 592
            + + T+  ++ +  ++  E   +   L  YY   M +N    + +PKF G+Y  + + GKN++    L+     P  FR+H +F+L    Y  ++S K       ++  L DF    PDG L++      +++  + D   L   +++ YSL + VHN + ++RE   E
Sbjct:  174 IFYVTRDDEFIIKTVQHKEAEFLQKLLPGYY---MNLNQNPRTLLPKFFGLYCIQ-SGGKNIR---ILVMNNVLPRVFRMHLKFDLKGSTYKRRASKKEREKSRPTFKDL-DFLQEVPDGLLLDTDTFSALVKTLQRDCLVLESFKIMDYSLLLGVHNLDQAEREQQTE 334
[Close]
BLAST of SMED30024217 vs. Ensembl Sea Lamprey
Match: pip5k1ab (phosphatidylinositol-4-phosphate 5-kinase, type I, alpha, b [Source:ZFIN;Acc:ZDB-GENE-041111-123])

HSP 1 Score: 55.4546 bits (132), Expect = 9.433e-9
Identity = 44/168 (26.19%), Postives = 78/168 (46.43%), Query Frame = 2 
Query:  119 LIFNTQYGKYSLHKIKDSEMCIITSYLKRYYQCSMGMNY---SFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFEL---FYDVKSSTKSSSYNGLN----DFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDE 592
            + F T   ++ +  +   E   +   L  YY   M +N    + +PKF G+Y  + + GKN++    ++     P   ++H +F+L    Y  ++S K    +       DF  +HPDG L++      + +  + D   L   +++ YSL I VHN + S+RE D E
Sbjct:  104 VFFVTSDDEFIIKTVMHKEAEFLQKLLPGYY---MNLNQNVRTLLPKFYGLYCVQ-SGGKNIR---LVVMNNLLPRAMKMHIKFDLKGSTYKRRASRKEREKSNPTFKDLDFIQDHPDGLLLDSDTYNAMSKTLQRDCLVLESFKIMDYSLLIGVHNLDQSQRERDAE 264
[Close]
BLAST of SMED30024217 vs. Ensembl Sea Lamprey
Match: pip5k1ab (phosphatidylinositol-4-phosphate 5-kinase, type I, alpha, b [Source:ZFIN;Acc:ZDB-GENE-041111-123])

HSP 1 Score: 55.4546 bits (132), Expect = 1.141e-8
Identity = 44/168 (26.19%), Postives = 78/168 (46.43%), Query Frame = 2 
Query:  119 LIFNTQYGKYSLHKIKDSEMCIITSYLKRYYQCSMGMNY---SFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFEL---FYDVKSSTKSSSYNGLN----DFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDE 592
            + F T   ++ +  +   E   +   L  YY   M +N    + +PKF G+Y  + + GKN++    ++     P   ++H +F+L    Y  ++S K    +       DF  +HPDG L++      + +  + D   L   +++ YSL I VHN + S+RE D E
Sbjct:  104 VFFVTSDDEFIIKTVMHKEAEFLQKLLPGYY---MNLNQNVRTLLPKFYGLYCVQ-SGGKNIR---LVVMNNLLPRAMKMHIKFDLKGSTYKRRASRKEREKSNPTFKDLDFIQDHPDGLLLDSDTYNAMSKTLQRDCLVLESFKIMDYSLLIGVHNLDQSQRERDAE 264
[Close]
BLAST of SMED30024217 vs. Ensembl Sea Lamprey
Match: pip5k1ab (phosphatidylinositol-4-phosphate 5-kinase, type I, alpha, b [Source:ZFIN;Acc:ZDB-GENE-041111-123])

HSP 1 Score: 54.6842 bits (130), Expect = 1.846e-8
Identity = 44/168 (26.19%), Postives = 78/168 (46.43%), Query Frame = 2 
Query:  119 LIFNTQYGKYSLHKIKDSEMCIITSYLKRYYQCSMGMNY---SFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFEL---FYDVKSSTKSSSYNGLN----DFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDE 592
            + F T   ++ +  +   E   +   L  YY   M +N    + +PKF G+Y  + + GKN++    ++     P   ++H +F+L    Y  ++S K    +       DF  +HPDG L++      + +  + D   L   +++ YSL I VHN + S+RE D E
Sbjct:   94 VFFVTSDDEFIIKTVMHKEAEFLQKLLPGYY---MNLNQNVRTLLPKFYGLYCVQ-SGGKNIR---LVVMNNLLPRAMKMHIKFDLKGSTYKRRASRKEREKSNPTFKDLDFIQDHPDGLLLDSDTYNAMSKTLQRDCLVLESFKIMDYSLLIGVHNLDQSQRERDAE 254
[Close]
BLAST of SMED30024217 vs. Ensembl Medaka
Match: pip5k1ca (phosphatidylinositol-4-phosphate 5-kinase type 1 gamma [Source:NCBI gene;Acc:101162441])

HSP 1 Score: 50.0618 bits (118), Expect = 4.350e-6
Identity = 43/161 (26.71%), Postives = 73/161 (45.34%), Query Frame = 2 
Query:  143 KYSLHKIKDSEMCIITSYLKRYYQCSMGMNY---SFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSST------------KSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRE 580
            K  +HK    E   +   L  YY   M +N    + +PKF G+Y  + + GKN++    ++  V  P   R+H    L YD+K ST               ++  L DF  + PDG +++      +++  + D   L   +++ YSL + VHN + + RE
Sbjct:  192 KTVMHK----EAEFLQKLLPGYY---MNLNQNPRTLLPKFFGLYCVQ-SGGKNIRV--VVMNNV-LPRVVRMH----LKYDLKGSTYKRRASKKEREKARPTFKDL-DFMQDTPDGLMLDQDTYNALVKTLQRDCLVLESFKIMDYSLLLGVHNMDQADRE 336
[Close]
BLAST of SMED30024217 vs. Ensembl Medaka
Match: pip5k1ca (phosphatidylinositol-4-phosphate 5-kinase type 1 gamma [Source:NCBI gene;Acc:101162441])

HSP 1 Score: 49.6766 bits (117), Expect = 5.071e-6
Identity = 43/161 (26.71%), Postives = 73/161 (45.34%), Query Frame = 2 
Query:  143 KYSLHKIKDSEMCIITSYLKRYYQCSMGMNY---SFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSST------------KSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRE 580
            K  +HK    E   +   L  YY   M +N    + +PKF G+Y  + + GKN++    ++  V  P   R+H    L YD+K ST               ++  L DF  + PDG +++      +++  + D   L   +++ YSL + VHN + + RE
Sbjct:  192 KTVMHK----EAEFLQKLLPGYY---MNLNQNPRTLLPKFFGLYCVQ-SGGKNIRV--VVMNNV-LPRVVRMH----LKYDLKGSTYKRRASKKEREKARPTFKDL-DFMQDTPDGLMLDQDTYNALVKTLQRDCLVLESFKIMDYSLLLGVHNMDQADRE 336
[Close]
BLAST of SMED30024217 vs. Ensembl Medaka
Match: pip5k1ca (phosphatidylinositol-4-phosphate 5-kinase type 1 gamma [Source:NCBI gene;Acc:101162441])

HSP 1 Score: 49.2914 bits (116), Expect = 6.762e-6
Identity = 43/161 (26.71%), Postives = 73/161 (45.34%), Query Frame = 2 
Query:  143 KYSLHKIKDSEMCIITSYLKRYYQCSMGMNY---SFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSST------------KSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRE 580
            K  +HK    E   +   L  YY   M +N    + +PKF G+Y  + + GKN++    ++  V  P   R+H    L YD+K ST               ++  L DF  + PDG +++      +++  + D   L   +++ YSL + VHN + + RE
Sbjct:  144 KTVMHK----EAEFLQKLLPGYY---MNLNQNPRTLLPKFFGLYCVQ-SGGKNIRVV--VMNNV-LPRVVRMH----LKYDLKGSTYKRRASKKEREKARPTFKDL-DFMQDTPDGLMLDQDTYNALVKTLQRDCLVLESFKIMDYSLLLGVHNMDQADRE 288
[Close]
BLAST of SMED30024217 vs. Planmine SMEST
Match: SMESG000065539.1 (SMESG000065539.1)

HSP 1 Score: 869.766 bits (2246), Expect = 0.000e+0
Identity = 429/429 (100.00%), Postives = 429/429 (100.00%), Query Frame = 2 
Query:   59 GSIVKYDLSFCAHSHFDKDRLIFNTQYGKYSLHKIKDSEMCIITSYLKRYYQCSMGMNYSFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSSTKSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDEIKNNRRTSVLPKMYKSSDAENHNVYRRASISPYRTPLDDNQRMKIGEKHPPKVNHHHSSSSDTASLIIIGSNRESKGRTSDLAAIMAKGFNKVNTTFLFNSAKLQCDFYKAKRHGAIFVYDVKGEHLAVFLSFGHIFDKTEDLYTYKSLLKSTFKCRCTRKITTIASYADGFENFLLQQAFRCVKPDMSFELWPDEINTPSDAEKKNAPENALDKWAHIKATLIXXXXXXXXXLFSNHQNISELPSSSEFI 1345
            GSIVKYDLSFCAHSHFDKDRLIFNTQYGKYSLHKIKDSEMCIITSYLKRYYQCSMGMNYSFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSSTKSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDEIKNNRRTSVLPKMYKSSDAENHNVYRRASISPYRTPLDDNQRMKIGEKHPPKVNHHHSSSSDTASLIIIGSNRESKGRTSDLAAIMAKGFNKVNTTFLFNSAKLQCDFYKAKRHGAIFVYDVKGEHLAVFLSFGHIFDKTEDLYTYKSLLKSTFKCRCTRKITTIASYADGFENFLLQQAFRCVKPDMSFELWPDEINTPSDAEKKNAPENALDKWAHIKATLISDSSSEDSSLFSNHQNISELPSSSEFI
Sbjct:  107 GSIVKYDLSFCAHSHFDKDRLIFNTQYGKYSLHKIKDSEMCIITSYLKRYYQCSMGMNYSFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSSTKSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDEIKNNRRTSVLPKMYKSSDAENHNVYRRASISPYRTPLDDNQRMKIGEKHPPKVNHHHSSSSDTASLIIIGSNRESKGRTSDLAAIMAKGFNKVNTTFLFNSAKLQCDFYKAKRHGAIFVYDVKGEHLAVFLSFGHIFDKTEDLYTYKSLLKSTFKCRCTRKITTIASYADGFENFLLQQAFRCVKPDMSFELWPDEINTPSDAEKKNAPENALDKWAHIKATLISDSSSEDSSLFSNHQNISELPSSSEFI 535
[Close]
BLAST of SMED30024217 vs. Planmine SMEST
Match: SMESG000065539.1 (SMESG000065539.1)

HSP 1 Score: 868.611 bits (2243), Expect = 0.000e+0
Identity = 429/429 (100.00%), Postives = 429/429 (100.00%), Query Frame = 2 
Query:   59 GSIVKYDLSFCAHSHFDKDRLIFNTQYGKYSLHKIKDSEMCIITSYLKRYYQCSMGMNYSFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSSTKSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDEIKNNRRTSVLPKMYKSSDAENHNVYRRASISPYRTPLDDNQRMKIGEKHPPKVNHHHSSSSDTASLIIIGSNRESKGRTSDLAAIMAKGFNKVNTTFLFNSAKLQCDFYKAKRHGAIFVYDVKGEHLAVFLSFGHIFDKTEDLYTYKSLLKSTFKCRCTRKITTIASYADGFENFLLQQAFRCVKPDMSFELWPDEINTPSDAEKKNAPENALDKWAHIKATLIXXXXXXXXXLFSNHQNISELPSSSEFI 1345
            GSIVKYDLSFCAHSHFDKDRLIFNTQYGKYSLHKIKDSEMCIITSYLKRYYQCSMGMNYSFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSSTKSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDEIKNNRRTSVLPKMYKSSDAENHNVYRRASISPYRTPLDDNQRMKIGEKHPPKVNHHHSSSSDTASLIIIGSNRESKGRTSDLAAIMAKGFNKVNTTFLFNSAKLQCDFYKAKRHGAIFVYDVKGEHLAVFLSFGHIFDKTEDLYTYKSLLKSTFKCRCTRKITTIASYADGFENFLLQQAFRCVKPDMSFELWPDEINTPSDAEKKNAPENALDKWAHIKATLISDSSSEDSSLFSNHQNISELPSSSEFI
Sbjct:   94 GSIVKYDLSFCAHSHFDKDRLIFNTQYGKYSLHKIKDSEMCIITSYLKRYYQCSMGMNYSFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSSTKSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDEIKNNRRTSVLPKMYKSSDAENHNVYRRASISPYRTPLDDNQRMKIGEKHPPKVNHHHSSSSDTASLIIIGSNRESKGRTSDLAAIMAKGFNKVNTTFLFNSAKLQCDFYKAKRHGAIFVYDVKGEHLAVFLSFGHIFDKTEDLYTYKSLLKSTFKCRCTRKITTIASYADGFENFLLQQAFRCVKPDMSFELWPDEINTPSDAEKKNAPENALDKWAHIKATLISDSSSEDSSLFSNHQNISELPSSSEFI 522
[Close]
BLAST of SMED30024217 vs. Planmine SMEST
Match: SMESG000065539.1 (SMESG000065539.1)

HSP 1 Score: 868.226 bits (2242), Expect = 0.000e+0
Identity = 429/429 (100.00%), Postives = 429/429 (100.00%), Query Frame = 2 
Query:   59 GSIVKYDLSFCAHSHFDKDRLIFNTQYGKYSLHKIKDSEMCIITSYLKRYYQCSMGMNYSFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSSTKSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDEIKNNRRTSVLPKMYKSSDAENHNVYRRASISPYRTPLDDNQRMKIGEKHPPKVNHHHSSSSDTASLIIIGSNRESKGRTSDLAAIMAKGFNKVNTTFLFNSAKLQCDFYKAKRHGAIFVYDVKGEHLAVFLSFGHIFDKTEDLYTYKSLLKSTFKCRCTRKITTIASYADGFENFLLQQAFRCVKPDMSFELWPDEINTPSDAEKKNAPENALDKWAHIKATLIXXXXXXXXXLFSNHQNISELPSSSEFI 1345
            GSIVKYDLSFCAHSHFDKDRLIFNTQYGKYSLHKIKDSEMCIITSYLKRYYQCSMGMNYSFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSSTKSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDEIKNNRRTSVLPKMYKSSDAENHNVYRRASISPYRTPLDDNQRMKIGEKHPPKVNHHHSSSSDTASLIIIGSNRESKGRTSDLAAIMAKGFNKVNTTFLFNSAKLQCDFYKAKRHGAIFVYDVKGEHLAVFLSFGHIFDKTEDLYTYKSLLKSTFKCRCTRKITTIASYADGFENFLLQQAFRCVKPDMSFELWPDEINTPSDAEKKNAPENALDKWAHIKATLISDSSSEDSSLFSNHQNISELPSSSEFI
Sbjct:   90 GSIVKYDLSFCAHSHFDKDRLIFNTQYGKYSLHKIKDSEMCIITSYLKRYYQCSMGMNYSFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFELFYDVKSSTKSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDEIKNNRRTSVLPKMYKSSDAENHNVYRRASISPYRTPLDDNQRMKIGEKHPPKVNHHHSSSSDTASLIIIGSNRESKGRTSDLAAIMAKGFNKVNTTFLFNSAKLQCDFYKAKRHGAIFVYDVKGEHLAVFLSFGHIFDKTEDLYTYKSLLKSTFKCRCTRKITTIASYADGFENFLLQQAFRCVKPDMSFELWPDEINTPSDAEKKNAPENALDKWAHIKATLISDSSSEDSSLFSNHQNISELPSSSEFI 518
[Close]
BLAST of SMED30024217 vs. Planmine SMEST
Match: SMESG000010509.1 (SMESG000010509.1)

HSP 1 Score: 53.9138 bits (128), Expect = 1.961e-7
Identity = 39/153 (25.49%), Postives = 70/153 (45.75%), Query Frame = 2 
Query:  143 KYSLHKIKDSEMCIITSYLKRYYQCSMGMNYSFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFEL-------FYDVKSSTKSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRE 580
            ++ +  +   E   +   L  YY   M    + +PKF G+Y Y+   GKN++   F++     PS  +IH++++L       +   K  +KSS      DFK NHP+G ++       ++     D   L    ++ +SL + +HN +  K E
Sbjct:  150 EFIIKTVDKKEAIFLQKLLPEYYINLMQHPRTLLPKFYGLYCYETM-GKNIR---FVIMNNLLPS-IKIHEKYDLKGSTYKRYASKKERSKSSPTLKDLDFKENHPNGLILEPETYDALMSTISRDCMVLESFSIMDFSLLLGIHNVDQYKLE 297
[Close]
BLAST of SMED30024217 vs. Planmine SMEST
Match: SMESG000010509.1 (SMESG000010509.1)

HSP 1 Score: 53.5286 bits (127), Expect = 2.052e-7
Identity = 39/153 (25.49%), Postives = 70/153 (45.75%), Query Frame = 2 
Query:  143 KYSLHKIKDSEMCIITSYLKRYYQCSMGMNYSFIPKFLGIYSYKINDGKNVKTTHFLLREVKFPSEFRIHKQFEL-------FYDVKSSTKSSSYNGLNDFKYNHPDGFLMNIAQRKKVIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRE 580
            ++ +  +   E   +   L  YY   M    + +PKF G+Y Y+   GKN++   F++     PS  +IH++++L       +   K  +KSS      DFK NHP+G ++       ++     D   L    ++ +SL + +HN +  K E
Sbjct:  150 EFIIKTVDKKEAIFLQKLLPEYYINLMQHPRTLLPKFYGLYCYETM-GKNIR---FVIMNNLLPS-IKIHEKYDLKGSTYKRYASKKERSKSSPTLKDLDFKENHPNGLILEPETYDALMSTISRDCMVLESFSIMDFSLLLGIHNVDQYKLE 297
The following BLAST results are available for this feature:
BLAST of SMED30024217 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024217 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ppk-14.281e-721.61PIP Kinase [Source:UniProtKB/TrEMBL;Acc:O01759][more]
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BLAST of SMED30024217 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PIP5K59B2.929e-925.15gene:FBgn0034789 transcript:FBtr0290095[more]
PIP5K59B2.996e-926.92gene:FBgn0034789 transcript:FBtr0071900[more]
PIP5K59B3.178e-926.92gene:FBgn0034789 transcript:FBtr0304923[more]
PIP5K59B3.598e-926.92gene:FBgn0034789 transcript:FBtr0304921[more]
PIP5K59B3.799e-925.15gene:FBgn0034789 transcript:FBtr0339762[more]
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BLAST of SMED30024217 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
pip5k1cb4.650e-725.00phosphatidylinositol-4-phosphate 5-kinase, type I,... [more]
pip5k1cb5.367e-725.00phosphatidylinositol-4-phosphate 5-kinase, type I,... [more]
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BLAST of SMED30024217 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pip5k1a1.839e-723.86phosphatidylinositol-4-phosphate 5-kinase, type I,... [more]
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BLAST of SMED30024217 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Pip5k1c9.401e-625.42phosphatidylinositol-4-phosphate 5-kinase, type 1 ... [more]
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BLAST of SMED30024217 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|O48709|PI5K3_ARATH7.137e-726.53Phosphatidylinositol 4-phosphate 5-kinase 3 OS=Ara... [more]
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BLAST of SMED30024217 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1B0GC281.954e-822.88PIPK domain-containing protein OS=Glossina morsita... [more]
A0A5K3ESS62.872e-822.82Uncharacterized protein OS=Mesocestoides corti OX=... [more]
G4LZK43.173e-825.30Phosphatidylinositol-4-phosphate 5-kinase type I, ... [more]
A0A5K4F7F63.211e-825.30Phosphatidylinositol-4-phosphate 5-kinase type I, ... [more]
A0A5K3ESR63.330e-825.91Uncharacterized protein OS=Mesocestoides corti OX=... [more]
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BLAST of SMED30024217 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
pip5k1cb6.201e-825.44phosphatidylinositol 4-phosphate 5-kinase type-1 g... [more]
pip5k1cb6.510e-825.44phosphatidylinositol 4-phosphate 5-kinase type-1 g... [more]
pip5k1cb7.497e-825.44phosphatidylinositol 4-phosphate 5-kinase type-1 g... [more]
pip5k1cb8.125e-825.44phosphatidylinositol 4-phosphate 5-kinase type-1 g... [more]
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BLAST of SMED30024217 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3
Match NameE-valueIdentityDescription
pip5k1ab9.433e-926.19phosphatidylinositol-4-phosphate 5-kinase, type I,... [more]
pip5k1ab1.141e-826.19phosphatidylinositol-4-phosphate 5-kinase, type I,... [more]
pip5k1ab1.846e-826.19phosphatidylinositol-4-phosphate 5-kinase, type I,... [more]
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BLAST of SMED30024217 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024217 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024217 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
pip5k1ca4.350e-626.71phosphatidylinositol-4-phosphate 5-kinase type 1 g... [more]
pip5k1ca5.071e-626.71phosphatidylinositol-4-phosphate 5-kinase type 1 g... [more]
pip5k1ca6.762e-626.71phosphatidylinositol-4-phosphate 5-kinase type 1 g... [more]
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BLAST of SMED30024217 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000065539.10.000e+0100.00SMESG000065539.1[more]
SMESG000065539.10.000e+0100.00SMESG000065539.1[more]
SMESG000065539.10.000e+0100.00SMESG000065539.1[more]
SMESG000010509.11.961e-725.49SMESG000010509.1[more]
SMESG000010509.12.052e-725.49SMESG000010509.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024217 ID=SMED30024217|Name=SMED30024217|organism=Schmidtea mediterranea sexual|type=transcript|length=1402bp
AGTCCTGGTTAACAATTGAATTTTAAAATTAAATTTCTTCTGAATGCCTC
CATTCTAGGGAAGCATCGTAAAGTATGATCTTTCGTTTTGTGCCCATTCT
CATTTCGACAAAGATCGTTTGATATTCAATACTCAATATGGAAAATATAG
TCTTCATAAAATCAAAGATTCGGAGATGTGCATCATCACCAGCTATCTGA
AAAGATATTATCAATGCTCAATGGGAATGAATTATTCTTTCATACCGAAA
TTTCTCGGAATATACTCTTATAAAATAAACGACGGAAAGAATGTGAAAAC
CACTCATTTTCTTCTCCGGGAAGTGAAATTTCCAAGCGAATTCAGAATAC
ACAAACAATTCGAATTGTTCTATGATGTCAAATCGTCAACAAAATCTTCC
TCCTACAACGGATTAAATGATTTCAAATATAATCATCCAGATGGTTTTCT
TATGAATATCGCTCAAAGAAAAAAAGTTATTGAAATTTTCGAACATGATT
TTGAATTTTTAAGGATTCTTCGCCTTCTGAAATATTCTTTACGAATCGCA
GTGCATAACTTCAATTTATCTAAAAGAGAACTGGATGATGAAATTAAAAA
TAACCGAAGGACATCTGTACTTCCTAAAATGTACAAATCTTCCGACGCTG
AAAATCATAACGTTTATAGAAGAGCTTCTATAAGCCCTTACAGAACTCCA
TTGGATGACAATCAGAGAATGAAAATTGGAGAAAAGCATCCTCCGAAAGT
TAACCACCATCATTCATCCTCATCTGACACCGCAAGTTTAATTATAATTG
GATCAAATAGAGAATCAAAAGGAAGGACCTCTGACCTGGCCGCTATAATG
GCGAAAGGGTTCAATAAAGTTAATACAACTTTTCTATTTAATTCTGCTAA
GTTACAATGTGATTTTTATAAAGCAAAGAGGCATGGAGCCATATTTGTTT
ACGATGTAAAGGGCGAACATTTAGCGGTATTTCTATCATTCGGTCATATT
TTTGATAAAACAGAGGATCTCTATACATATAAATCACTTTTAAAGTCCAC
TTTCAAATGTCGATGTACGAGAAAAATTACAACTATAGCAAGTTATGCAG
ATGGATTCGAAAACTTTCTTTTGCAACAGGCTTTTAGATGTGTCAAGCCA
GATATGAGTTTCGAGCTCTGGCCCGACGAAATTAACACTCCAAGCGACGC
TGAAAAAAAAAATGCACCAGAGAATGCCTTGGATAAATGGGCTCATATCA
AAGCTACCCTTATCAGTGACAGTTCTTCTGAAGATTCTTCTTTATTCTCT
AATCATCAAAACATATCAGAATTGCCGTCTTCATCGGAATTTATTTAACA
AAATTAAATTTTGTTTCAATGAACTATTAAAGTAACGAATAGCGGAAATA
AG
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protein sequence of SMED30024217-orf-1

>SMED30024217-orf-1 ID=SMED30024217-orf-1|Name=SMED30024217-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=391bp
MCIITSYLKRYYQCSMGMNYSFIPKFLGIYSYKINDGKNVKTTHFLLREV
KFPSEFRIHKQFELFYDVKSSTKSSSYNGLNDFKYNHPDGFLMNIAQRKK
VIEIFEHDFEFLRILRLLKYSLRIAVHNFNLSKRELDDEIKNNRRTSVLP
KMYKSSDAENHNVYRRASISPYRTPLDDNQRMKIGEKHPPKVNHHHSSSS
DTASLIIIGSNRESKGRTSDLAAIMAKGFNKVNTTFLFNSAKLQCDFYKA
KRHGAIFVYDVKGEHLAVFLSFGHIFDKTEDLYTYKSLLKSTFKCRCTRK
ITTIASYADGFENFLLQQAFRCVKPDMSFELWPDEINTPSDAEKKNAPEN
ALDKWAHIKATLISDSSSEDSSLFSNHQNISELPSSSEFI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000208testis
PLANA:0000227germ cell
PLANA:0002030early male germ cell
PLANA:0002089reproductive organ
PLANA:0003304testis primordium
Vocabulary: INTERPRO
TermDefinition
IPR023610PInositol-4-P-5-kinase
IPR027484PInositol-4-P-5-kinase_N
IPR027483PInositol-4-P-5-kinase_C
IPR002498PInositol-4-P-5-kinase_core
Vocabulary: biological process
TermDefinition
GO:0046488phosphatidylinositol metabolic process
GO:0016310phosphorylation
GO:0046854phosphatidylinositol phosphorylation
Vocabulary: molecular function
TermDefinition
GO:0016307phosphatidylinositol phosphate kinase activity
GO:0016301kinase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027483Phosphatidylinositol-4-phosphate 5-kinase, C-terminalGENE3DG3DSA:3.30.810.10coord: 52..172
e-value: 7.2E-6
score: 27.7
IPR002498Phosphatidylinositol-4-phosphate 5-kinase, corePFAMPF01504PIP5Kcoord: 4..178
e-value: 2.3E-12
score: 46.7
IPR002498Phosphatidylinositol-4-phosphate 5-kinase, corePROSITEPS51455PIPKcoord: 1..184
score: 13.176
IPR023610Phosphatidylinositol-4-phosphate 5-kinasePANTHERPTHR23086PHOSPHATIDYLINOSITOL-4-PHOSPHATE 5-KINASEcoord: 19..324
NoneNo IPR availablePANTHERPTHR23086:SF101LP03320P-RELATEDcoord: 19..324
NoneNo IPR availableCDDcd00139PIPKccoord: 8..134
e-value: 9.26333E-11
score: 59.5087
NoneNo IPR availableSUPERFAMILYSSF56104SAICAR synthase-likecoord: 4..146