SMED30024204

Overview
NameSMED30024204
Smed IDSMED30024204
Length (bp)600
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30024204 (SMED30024204) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30024204 (SMED30024204) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30024204 (SMED30024204) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30024204 (SMED30024204) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30024204

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30024204h1SMcG0018988 Contig1248GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30024204h1SMcG0018988 Contig1248GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
Smed sexual biotypeSMED30024204h1SMcG0018988 Contig35448uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30024204h1SMcG0018988 Contig35448newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30024204h1SMcG0018988 dd_75dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
intestinal phagocyteSMED30024204h1SMcG0018988 dd_75dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
gutSMED30024204h1SMcG0018988 dd_Smed_v4_75_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30024204h1SMcG0018988 dd_Smed_v4_75_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30024204h1SMcG0018988 dd_Smed_v6_75_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30024204 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000230:187423..188072 -2979
Homology
[Close]
BLAST of SMED30024204 vs. Ensembl Fly
Match: CG44250 (gene:FBgn0265185 transcript:FBtr0339104)

HSP 1 Score: 52.7582 bits (125), Expect = 3.804e-8
Identity = 42/139 (30.22%), Postives = 59/139 (42.45%), Query Frame = 3 
Query:   81 MDQTDNETDWVYSDSGKKVPG-AVYAGLKS-----YIIRAIHDDQRIIGTLIDGEDFARVAYNGAVHKDYAYQVLVNTKTPDCSKLVWKFTTGKIIPEGAIRGGHD-SSIMLFVGRAVINGELLVGSFRVDSGILYTGF 476
            MD T     WV+S     +P  AV  G  S     Y+ R+ H+ + +   ++  +  A VAY GA H    Y+VLV           W  ++   +P  A+R G   +   L+VGR    G L VG      G LY  F
Sbjct:    1 MDNT-----WVHSSPYSPLPPYAVIGGHDSDRTPIYVGRSFHEGENLPAKVVPSKGCAYVAYGGAEHTKTHYEVLVG------QGFAWVPSSSGGVPPNAVRSGTTRTGEPLYVGRGHHAGSLTVGKVHPSHGCLYIPF 128

HSP 2 Score: 45.8246 bits (107), Expect = 7.183e-6
Identity = 35/129 (27.13%), Postives = 51/129 (39.53%), Query Frame = 3 
Query:  108 WVYSDSGKKVPGAVYAGLKS-----YIIRAIHDDQRIIGTLIDGEDFARVAYNGAVHKDYAYQVLVNTKTPDCSKLVWKFTTGKIIPEGAIRGGHDS-SIMLFVGRAVINGELLVGSFRVDSGILYTGF 476
            WV S SG   P AV +G        Y+ R  H     +G +        + + G   +   Y+VL+  +       VW   +    P GA+  GHDS    ++ GRA+  GE+L        G  Y  F
Sbjct:   77 WVPSSSGGVPPNAVRSGTTRTGEPLYVGRGHHAGSLTVGKVHPSHGCLYIPFGGQEVRINTYEVLIKQQ-----HDVWVPASPSYTPPGAVIAGHDSDRTPIYAGRAMHEGEMLPAKVVPSKGTAYVCF 200
[Close]
BLAST of SMED30024204 vs. Ensembl Fly
Match: CG44250 (gene:FBgn0265185 transcript:FBtr0339105)

HSP 1 Score: 52.373 bits (124), Expect = 5.512e-8
Identity = 39/133 (29.32%), Postives = 57/133 (42.86%), Query Frame = 3 
Query:   99 ETDWVYSDSGKKVPG-AVYAGLKS-----YIIRAIHDDQRIIGTLIDGEDFARVAYNGAVHKDYAYQVLVNTKTPDCSKLVWKFTTGKIIPEGAIRGGHD-SSIMLFVGRAVINGELLVGSFRVDSGILYTGF 476
            +  WV+S     +P  AV  G  S     Y+ R+ H+ + +   ++  +  A VAY GA H    Y+VLV           W  ++   +P  A+R G   +   L+VGR    G L VG      G LY  F
Sbjct:   13 DNTWVHSSPYSPLPPYAVIGGHDSDRTPIYVGRSFHEGENLPAKVVPSKGCAYVAYGGAEHTKTHYEVLVG------QGFAWVPSSSGGVPPNAVRSGTTRTGEPLYVGRGHHAGSLTVGKVHPSHGCLYIPF 139

HSP 2 Score: 45.8246 bits (107), Expect = 7.730e-6
Identity = 35/129 (27.13%), Postives = 51/129 (39.53%), Query Frame = 3 
Query:  108 WVYSDSGKKVPGAVYAGLKS-----YIIRAIHDDQRIIGTLIDGEDFARVAYNGAVHKDYAYQVLVNTKTPDCSKLVWKFTTGKIIPEGAIRGGHDS-SIMLFVGRAVINGELLVGSFRVDSGILYTGF 476
            WV S SG   P AV +G        Y+ R  H     +G +        + + G   +   Y+VL+  +       VW   +    P GA+  GHDS    ++ GRA+  GE+L        G  Y  F
Sbjct:   88 WVPSSSGGVPPNAVRSGTTRTGEPLYVGRGHHAGSLTVGKVHPSHGCLYIPFGGQEVRINTYEVLIKQQ-----HDVWVPASPSYTPPGAVIAGHDSDRTPIYAGRAMHEGEMLPAKVVPSKGTAYVCF 211
[Close]
BLAST of SMED30024204 vs. Ensembl Fly
Match: CG32633 (gene:FBgn0052633 transcript:FBtr0073800)

HSP 1 Score: 50.0618 bits (118), Expect = 3.040e-7
Identity = 36/115 (31.30%), Postives = 54/115 (46.96%), Query Frame = 3 
Query:  105 DWVYSDSGKKVPGAVYAGLKS-----YIIRAIHDDQRIIGTLIDGEDFARVAYNGAVHKDYAYQVLVNTKTPDCSKLVWKFTTGKIIPEGAIRGGHDSS-IMLFVGRAVINGELL 431
            +W+ +++G+  PGAV  G        Y  R  H     +G +        + Y+    K +AY+VL       C    W  TT   IP+GA+  GHDS+   ++VGR   NG+LL
Sbjct:   76 EWLSAENGEVPPGAVKVGRNVDGEYLYAGRGYHAGSLTMGKVHPSHGCLYIPYDSDEVKIFAYEVL-------CQPERWIDTTATNIPDGALVAGHDSNGDTIYVGRVFRNGDLL 183
[Close]
BLAST of SMED30024204 vs. Ensembl Fly
Match: CG32633 (gene:FBgn0052633 transcript:FBtr0310431)

HSP 1 Score: 50.0618 bits (118), Expect = 3.040e-7
Identity = 36/115 (31.30%), Postives = 54/115 (46.96%), Query Frame = 3 
Query:  105 DWVYSDSGKKVPGAVYAGLKS-----YIIRAIHDDQRIIGTLIDGEDFARVAYNGAVHKDYAYQVLVNTKTPDCSKLVWKFTTGKIIPEGAIRGGHDSS-IMLFVGRAVINGELL 431
            +W+ +++G+  PGAV  G        Y  R  H     +G +        + Y+    K +AY+VL       C    W  TT   IP+GA+  GHDS+   ++VGR   NG+LL
Sbjct:   76 EWLSAENGEVPPGAVKVGRNVDGEYLYAGRGYHAGSLTMGKVHPSHGCLYIPYDSDEVKIFAYEVL-------CQPERWIDTTATNIPDGALVAGHDSNGDTIYVGRVFRNGDLL 183
[Close]
BLAST of SMED30024204 vs. Ensembl Fly
Match: CG10527 (gene:FBgn0034583 transcript:FBtr0071541)

HSP 1 Score: 47.7506 bits (112), Expect = 2.072e-6
Identity = 37/125 (29.60%), Postives = 50/125 (40.00%), Query Frame = 3 
Query:  108 WVYSDSGKKVPGAVYAGLKS----YIIRAIHDDQRIIGTLIDGEDFARVAYNGAVHKDYAYQVLVNTKTPDCSKLVWKFTTGKIIPEGAIRGGHDSS-IMLFVGRAVINGELLVGSFRVDSGILY 467
            WV + +G+  P A+  G  S    YI RA H+   I G L        VA+ G  H    Y+VL            W       IP  A+  G  +    LF+GRA  +G + VG  +   G  Y
Sbjct:  159 WVPAANGEVPPNALEGGFDSSEQLYIARARHEGDLIPGKLHPSHGVTYVAWGGGEHGHAEYEVLC------AGGGQWLPVDAGNIPPNALPAGETAEGEPLFIGRATHDGTITVGKVQPSHGCCY 277
[Close]
BLAST of SMED30024204 vs. TrEMBL
Match: A0A2I9LP56 (Methyltransferase-like protein OS=Centruroides hentzi OX=88313 PE=4 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 8.506e-15
Identity = 51/146 (34.93%), Postives = 73/146 (50.00%), Query Frame = 3 
Query:  102 TDWVYSDSGKKVPGAVYAGLKS----YIIRAIHDDQRIIGTLIDGEDFARVAYNGAVHKDYAYQVLVNTKTPDCSKLVWKFTTGKIIPEGAIRGGHDS-SIMLFVGRAVINGELLVGSFRVDSGILYTGFNS-VHSVAGCDIL-CK 518
             +WV + SG   P AV AG       Y+ RA H+   I G L+       V + G  H  ++YQVL N   P+  +L W+FT+G  IP GA++GG  +    L++GR    G + VG  +   G+LY  F    HS    ++L CK
Sbjct:   13 AEWVPASSGHVPPCAVQAGYDVSDTLYVARAHHNGDNIPGKLVPSHGCCYVPWGGEEHSHHSYQVLCN---PEGVQLEWRFTSGGDIPPGAVQGGMTADGEPLYIGRHDHEGTMTVGKVQPSHGVLYIAFGGREHSYPDYEVLVCK 155
[Close]
BLAST of SMED30024204 vs. TrEMBL
Match: Q686X3 (CG10527-like methyltransferase (Fragment) OS=Mesobuthus eupeus OX=34648 GN=ttrans PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 6.736e-13
Identity = 39/104 (37.50%), Postives = 56/104 (53.85%), Query Frame = 3 
Query:  168 YIIRAIHDDQRIIGTLIDGEDFARVAYNGAVHKDYAYQVLVNTKTPDCSKLVWKFTTGKIIPEGAIRGGHDSS-IMLFVGRAVINGELLVGSFRVDSGILYTGF 476
            Y+ RA H+   I G L+       V + G  H+ + YQVL N   P+  +L W+FT+G  IP GAI+GG  S    LF+GR   +G + +G  +   G+LY  F
Sbjct:   11 YVARAHHNGDNIPGKLVPSHGCCYVPWGGEEHRHHDYQVLCN---PEGIQLEWRFTSGGDIPPGAIQGGMTSEGEPLFIGRHEHDGTMTIGKVQPSHGVLYISF 111
[Close]
BLAST of SMED30024204 vs. TrEMBL
Match: A0A1W7RA66 (Methyltransferase-like protein OS=Hadrurus spadix OX=141984 PE=4 SV=1)

HSP 1 Score: 71.2478 bits (173), Expect = 5.153e-12
Identity = 40/130 (30.77%), Postives = 64/130 (49.23%), Query Frame = 3 
Query:  102 TDWVYSDSGKKVPGAVYAGLKS----YIIRAIHDDQRIIGTLIDGEDFARVAYNGAVHKDYAYQVLVNTKTPDCSKLVWKFTTGKIIPEGAIRGGHDS-SIMLFVGRAVINGELLVGSFRVDSGILYTGF 476
             +W++  SG+  P AV  G       Y+ RA H+   I G L+       V++ G  H  ++Y+VL N   P  S+L WK+  G  +P GA++GG  +    L++GR    G + VG  +    +LY  +
Sbjct:   13 AEWLFGSSGQVPPCAVQGGYDGGDTIYVARAHHNGDNIPGKLLPSHGCVYVSWGGEEHSHHSYEVLCN---PQGSQLEWKYCGGGDVPSGAVQGGTTADGEPLYIGRHEHEGTMTVGKVQPSHQVLYIAY 139
[Close]
BLAST of SMED30024204 vs. TrEMBL
Match: Q686V2 (CG10527-like methyltransferase (Fragment) OS=Mesobuthus gibbosus OX=123226 GN=mettrans PE=4 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 7.497e-12
Identity = 36/104 (34.62%), Postives = 54/104 (51.92%), Query Frame = 3 
Query:  168 YIIRAIHDDQRIIGTLIDGEDFARVAYNGAVHKDYAYQVLVNTKTPDCSKLVWKFTTGKIIPEGAIRGGHDS-SIMLFVGRAVINGELLVGSFRVDSGILYTGF 476
            Y+ RA H+   I G L+       V + G  H+ + YQVL N   P+  +L W+F +G  IP GAI+GG  S    L++GR    G + +G  +   G++Y  F
Sbjct:   11 YVARAHHNGHNIPGKLVPSHGCCYVPWGGEEHRHHEYQVLCN---PEGMQLEWRFASGGDIPPGAIQGGMTSDGEPLYIGRHEHEGTMTIGKIQPSHGVVYISF 111
[Close]
BLAST of SMED30024204 vs. TrEMBL
Match: Q686V7 (CG10527-like methyltransferase (Fragment) OS=Mesobuthus gibbosus OX=123226 GN=mettrans PE=4 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 9.095e-12
Identity = 39/113 (34.51%), Postives = 58/113 (51.33%), Query Frame = 3 
Query:  168 YIIRAIHDDQRIIGTLIDGEDFARVAYNGAVHKDYAYQVLVNTKTPDCSKLVWKFTTGKIIPEGAIRGGHDS-SIMLFVGRAVINGELLVGSFRVDSGILYTGF-NSVHSVAG 500
            Y+ RA H+   I G L+       V + G  H+ + YQVL N   P+  +L W+F +G  IP GAI+GG  S    L++GR    G + +G  +   G++Y  F    HS +G
Sbjct:   11 YVARAHHNGDNIPGKLVPSHGCCYVPWGGEEHRHHEYQVLCN---PEGMQLEWRFASGGDIPPGAIQGGMTSDGEPLYIGRHEHEGTMTIGKIQPSHGVVYISFGGGEHSYSG 120
[Close]
BLAST of SMED30024204 vs. Planmine SMEST
Match: SMESG000027140.1 (SMESG000027140.1)

HSP 1 Score: 306.22 bits (783), Expect = 5.025e-108
Identity = 149/149 (100.00%), Postives = 149/149 (100.00%), Query Frame = 3 
Query:   81 MDQTDNETDWVYSDSGKKVPGAVYAGLKSYIIRAIHDDQRIIGTLIDGEDFARVAYNGAVHKDYAYQVLVNTKTPDCSKLVWKFTTGKIIPEGAIRGGHDSSIMLFVGRAVINGELLVGSFRVDSGILYTGFNSVHSVAGCDILCKVDQ 527
            MDQTDNETDWVYSDSGKKVPGAVYAGLKSYIIRAIHDDQRIIGTLIDGEDFARVAYNGAVHKDYAYQVLVNTKTPDCSKLVWKFTTGKIIPEGAIRGGHDSSIMLFVGRAVINGELLVGSFRVDSGILYTGFNSVHSVAGCDILCKVDQ
Sbjct:    1 MDQTDNETDWVYSDSGKKVPGAVYAGLKSYIIRAIHDDQRIIGTLIDGEDFARVAYNGAVHKDYAYQVLVNTKTPDCSKLVWKFTTGKIIPEGAIRGGHDSSIMLFVGRAVINGELLVGSFRVDSGILYTGFNSVHSVAGCDILCKVDQ 149
[Close]
BLAST of SMED30024204 vs. Planmine SMEST
Match: SMESG000027142.1 (SMESG000027142.1)

HSP 1 Score: 142.895 bits (359), Expect = 2.199e-43
Identity = 69/147 (46.94%), Postives = 95/147 (64.63%), Query Frame = 3 
Query:   84 DQTDNETDWVYSDSGKKVPGAVYAGLKSYIIRAIHDDQRIIGTLIDGEDFARVAYNGAVHKDYAYQVLVNTKTPDCSKLVWKFTTGKIIPEGAIRGGHDSSIMLFVGRAVINGELLVGSFRVDSGILYTGF-NSVHSVAGCDILCKV 521
            D  D +T W+Y++SGK     VYAGL   + RAI D  R++G+L++GE    ++ N  V + Y YQV V       ++L WK+ +GKI+PEGAI GG D+  +LF+GR  I+G LLVGS+R D G +  G+ N VH + GCDILCKV
Sbjct:    9 DIVDGQTKWLYNESGKYQEDTVYAGLNCRVARAICDGNRLVGSLLEGEKCVYLSSNNTVFEQYEYQVFVEENNCKNTRLEWKWASGKIVPEGAIVGGSDAGNVLFIGRTAIDGRLLVGSYRADKGKITVGYKNRVHIIPGCDILCKV 155
[Close]
BLAST of SMED30024204 vs. Planmine SMEST
Match: SMESG000027124.1 (SMESG000027124.1)

HSP 1 Score: 130.568 bits (327), Expect = 3.560e-38
Identity = 66/148 (44.59%), Postives = 90/148 (60.81%), Query Frame = 3 
Query:   84 DQTDNETDWVYSDSGKKVPGAVYAGLKSYIIRAIHDDQRIIGTLIDGEDFARVAYNGAVHKDYAYQVLVNTKTPDCSKLVWKFTTGKIIPEGAIRGGHDSSIMLFVGRAVINGELLVGSFRVDSGILYTGFN-SVHSVAGCDILCKVD 524
            D+ DN+  W Y   G+    AVY G  SYIIRAIH +Q  IGT++ G+    V +    H  Y YQ++V  +    +   WK+T G  +PEGAI  G D++ +LFVGRA  NG+LL+GS+R D+GI+Y  +   V    GCDILC+VD
Sbjct:   45 DEVDNKGMWQYGQKGEIPDKAVYGGCDSYIIRAIHKEQTFIGTIVRGDLCGHVPFRKWSHSKYIYQIMVENR----NIFEWKWTDGTFLPEGAIIAGEDNATVLFVGRAAYNGQLLIGSYRPDNGIVYLPYKGEVRERVGCDILCRVD 188
[Close]
BLAST of SMED30024204 vs. Planmine SMEST
Match: SMESG000074396.1 (SMESG000074396.1)

HSP 1 Score: 82.8037 bits (203), Expect = 5.040e-20
Identity = 44/139 (31.65%), Postives = 74/139 (53.24%), Query Frame = 3 
Query:  105 DWVYSDSGKKVPGAVYAGLKSYIIRAIHDDQRIIGTLIDGEDFARVAYNGAVHKDYAYQVLVNTKTPDCSK-LVWKFTTGKIIPEGAIRGGHDSSIMLFVGRAVINGELLVGSFRVDSGILYTGF-NSVHSVAGCDILC 515
             WV +++G   P AV AG ++++ RA HD Q I G  ++G +   V++ G  H    Y++LV +      K   WK+T G+ +P+GA+ GG +    L+V R+ I  +++VG +    G  Y  +    H V   ++LC
Sbjct:   17 SWVKANNGSIPPKAVRAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLC 155
[Close]
BLAST of SMED30024204 vs. Planmine SMEST
Match: SMESG000074378.1 (SMESG000074378.1)

HSP 1 Score: 82.8037 bits (203), Expect = 5.040e-20
Identity = 44/139 (31.65%), Postives = 74/139 (53.24%), Query Frame = 3 
Query:  105 DWVYSDSGKKVPGAVYAGLKSYIIRAIHDDQRIIGTLIDGEDFARVAYNGAVHKDYAYQVLVNTKTPDCSK-LVWKFTTGKIIPEGAIRGGHDSSIMLFVGRAVINGELLVGSFRVDSGILYTGF-NSVHSVAGCDILC 515
             WV +++G   P AV AG ++++ RA HD Q I G  ++G +   V++ G  H    Y++LV +      K   WK+T G+ +P+GA+ GG +    L+V R+ I  +++VG +    G  Y  +    H V   ++LC
Sbjct:   17 SWVKANNGSIPPKAVRAGGQTFVARAKHDGQYIPGKFVEGYNEVFVSFGGVEHAKNYYEILVESSMLGGHKGYEWKWTNGENVPKGALVGGLNDGAPLYVARSFIEDQMVVGKYYPQHGCAYMPYGGEEHRVENAEVLC 155
The following BLAST results are available for this feature:
BLAST of SMED30024204 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024204 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024204 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG442503.804e-830.22gene:FBgn0265185 transcript:FBtr0339104[more]
CG442505.512e-829.32gene:FBgn0265185 transcript:FBtr0339105[more]
CG326333.040e-731.30gene:FBgn0052633 transcript:FBtr0073800[more]
CG326333.040e-731.30gene:FBgn0052633 transcript:FBtr0310431[more]
CG105272.072e-629.60gene:FBgn0034583 transcript:FBtr0071541[more]
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BLAST of SMED30024204 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024204 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024204 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024204 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024204 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2I9LP568.506e-1534.93Methyltransferase-like protein OS=Centruroides hen... [more]
Q686X36.736e-1337.50CG10527-like methyltransferase (Fragment) OS=Mesob... [more]
A0A1W7RA665.153e-1230.77Methyltransferase-like protein OS=Hadrurus spadix ... [more]
Q686V27.497e-1234.62CG10527-like methyltransferase (Fragment) OS=Mesob... [more]
Q686V79.095e-1234.51CG10527-like methyltransferase (Fragment) OS=Mesob... [more]
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BLAST of SMED30024204 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024204 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024204 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024204 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024204 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024204 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000027140.15.025e-108100.00SMESG000027140.1[more]
SMESG000027142.12.199e-4346.94SMESG000027142.1[more]
SMESG000027124.13.560e-3844.59SMESG000027124.1[more]
SMESG000074396.15.040e-2031.65SMESG000074396.1[more]
SMESG000074378.15.040e-2031.65SMESG000074378.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024204 ID=SMED30024204|Name=SMED30024204|organism=Schmidtea mediterranea sexual|type=transcript|length=600bp
TTGCGGTCGTTTATTACTAGTTAATAATGCCGTTATGAACATGATTTTCG
AATAGAGCTCAGCAATAATTTCACTACAAAATGGACCAAACTGATAACGA
AACAGACTGGGTTTACAGTGACAGTGGAAAGAAAGTGCCCGGCGCCGTAT
ATGCTGGTCTGAAATCCTACATTATTAGAGCGATACATGACGATCAGAGA
ATTATTGGTACCCTTATAGATGGTGAAGATTTTGCTCGTGTTGCCTACAA
TGGAGCAGTTCACAAAGATTATGCTTATCAAGTGCTAGTAAATACCAAAA
CTCCTGACTGCAGTAAATTGGTGTGGAAATTCACAACAGGGAAAATTATT
CCAGAAGGAGCCATAAGAGGGGGTCACGATAGTTCAATTATGCTATTTGT
TGGACGCGCGGTTATTAATGGTGAACTTCTGGTAGGTTCATTCAGAGTCG
ACAGTGGGATACTTTATACTGGTTTTAACTCGGTACACTCTGTGGCAGGA
TGCGATATTTTGTGTAAAGTTGATCAGTGAATACTTAGAACTTGAAATGT
TGATGTTTCTGATTGATTACTGATTTAATTCTCAATAAATATTATTGACT
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protein sequence of SMED30024204-orf-1

>SMED30024204-orf-1 ID=SMED30024204-orf-1|Name=SMED30024204-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=150bp
MDQTDNETDWVYSDSGKKVPGAVYAGLKSYIIRAIHDDQRIIGTLIDGED
FARVAYNGAVHKDYAYQVLVNTKTPDCSKLVWKFTTGKIIPEGAIRGGHD
SSIMLFVGRAVINGELLVGSFRVDSGILYTGFNSVHSVAGCDILCKVDQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
Vocabulary: INTERPRO
TermDefinition
IPR006616DM9_repeat
IPR024518DUF3421
Vocabulary: molecular function
TermDefinition
GO:0008168methyltransferase activity
GO:0016740transferase activity
Vocabulary: biological process
TermDefinition
GO:0032259methylation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006616DM9 repeatSMARTSM00696dm9coord: 79..147
e-value: 0.011
score: 1.3
coord: 7..72
e-value: 0.0011
score: 9.7
IPR024518Domain of unknown function DUF3421PFAMPF11901DUF3421coord: 28..133
e-value: 1.4E-16
score: 60.5
NoneNo IPR availablePANTHERPTHR31649FAMILY NOT NAMEDcoord: 8..134