SMED30024203

Overview
NameSMED30024203
Smed IDSMED30024203
Length (bp)451
Neoblast Clusters

Zeng et. al., 2018




 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30024203

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
cephalic gangliaSMED30024203h1SMcG0017034 dd_Smed_v4_6570_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30024203h1SMcG0017034 dd_Smed_v4_6570_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30024203h1SMcG0017034 dd_Smed_v4_4737_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30024203h1SMcG0017034 dd_Smed_v4_4737_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30024203h1SMcG0017034 dd_Smed_v4_6570_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30024203h1SMcG0017034 dd_Smed_v4_4737_0_1dd_Smed_v4PMID:30962434
Cote et al., 2019
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30024203 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.004501:8536..16606 -2007
Homology
BLAST of SMED30024203 vs. Planmine SMEST
Match: SMESG000066354.1 (SMESG000066354.1)

HSP 1 Score: 114.775 bits (286), Expect = 3.424e-30
Identity = 123/150 (82.00%), Postives = 129/150 (86.00%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCPA 451
            EPT+CP MKD+CTNP+VA +CK TC SST+   ST  SS SST  S+        EPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMSTEPTICP MKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLCPA
Sbjct: 1059 EPTLCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPIST--------EPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMSTEPTICPTMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLCPA 1200          

HSP 2 Score: 103.219 bits (256), Expect = 4.561e-26
Identity = 99/155 (63.87%), Postives = 115/155 (74.19%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPTVCPDMKDMC NPS+AKLCK TCASST  KSSTSGSS SST  S+        EPT+CP MKD+C NP+V+ +CKKTC S+T+  S ++         STEPT+CPAMKDLCTNPAV+VMCKKTC SST+   S+  +S SS P STEPTLCP
Sbjct:  965 EPTVCPDMKDMCINPSIAKLCKKTCASSTKSKSSTSGSSVSSTPTST--------EPTVCPDMKDMCTNPSVAKLCKKTCGSTTTGKSSTNVSSVSSTPKSTEPTLCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPISTEPTLCP 1111          

HSP 3 Score: 101.293 bits (251), Expect = 1.668e-25
Identity = 104/156 (66.67%), Postives = 122/156 (78.21%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCPA 451
            EPTVCPDMKDMCTNPSVAKLCK TCA STTPKSS+ GSS SST  S+        EPT+CP MKD+C+NP+++ +CKKTCA      SSTS SS+SST  STEPT+CP MKD+CTNP+V+ +CKKTC S+TT KSS+  +S SS P STEPTLCPA
Sbjct:  918 EPTVCPDMKDMCTNPSVAKLCKKTCAFSTTPKSSSRGSSISSTQIST--------EPTVCPDMKDMCINPSIAKLCKKTCASSTKSKSSTSGSSVSSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCGSTTTGKSSTNVSSVSSTPKSTEPTLCPA 1065          

HSP 4 Score: 98.5969 bits (244), Expect = 1.487e-24
Identity = 110/162 (67.90%), Postives = 130/162 (80.25%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA-------------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPTVCPDMKDMCTNPSVAKLCK TCASSTTPKSSTSGSSKSSTSG SISSTPISTEPT+CP MKD+C NP+V+ +CKKTCA              S+++ S  S+   TEPT+CP MKD+CTNP+V+ +CKKTCASSTT +SS++ +S+SS   STEPT+CP
Sbjct:  567 EPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGPSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCASSTTQESSTSGSSKSSTSMSTEPTVCP 728          

HSP 5 Score: 97.4413 bits (241), Expect = 3.728e-24
Identity = 96/163 (58.90%), Postives = 115/163 (70.55%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA--------------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPTVCPDMKDMCTNPSVAKLCKNTCA         S ++  S++  S  S+   TEPT+CP MKD+C NP+V+ +CKKTCA              SSTS SS+SST +STEPT+CP MKD+CTNP+V+ +CKKTCA STT KSSS  +S SS   STEPT+CP
Sbjct:  817 EPTVCPDMKDMCTNPSVAKLCKNTCA---------SSTTPKSSTKGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCAFSTTPKSSSRGSSISSTQISTEPTVCP 970          

HSP 6 Score: 95.5153 bits (236), Expect = 1.687e-23
Identity = 74/128 (57.81%), Postives = 89/128 (69.53%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPT+CP MKD+C NP+V+ +CK TCASST+  S +S    +              EPT+CP MKDLC NPAVS++CKKTCASST+  S S+         STEPT+CPAMKDLCTNPAVS+MCKKTCA
Sbjct: 1106 EPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMST--------------EPTICPTMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLCPAMKDLCTNPAVSIMCKKTCA 1219          

HSP 7 Score: 95.1301 bits (235), Expect = 2.436e-23
Identity = 99/155 (63.87%), Postives = 120/155 (77.42%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX------EPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPTVCPDMKDMCTNPSVAKLCK TCASSTT +SSTSGSSKSSTS S+        EPT+CP MK++C NP+V+ +CKKTCAS+T+  S +S    +      EPT+CP MKD+CTNP+V+ +CKKTCASSTT KSS++ +S SS   STEPT+CP
Sbjct:  676 EPTVCPDMKDMCTNPSVAKLCKKTCASSTTQESSTSGSSKSSTSMST--------EPTVCPDMKEMCTNPSVAKLCKKTCASTTTPKSSTSGSSISSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCASSTTSKSSTSGSSISSTQISTEPTVCP 822          

HSP 8 Score: 94.7449 bits (234), Expect = 3.553e-23
Identity = 80/144 (55.56%), Postives = 98/144 (68.06%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX----------------------EPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPT+CP MKD+CTNP+V+ +CK TCASSTT KSS++ +S+SS   S+        EPTLCP MKDLC NPAVS++CKKTCAS+T+  S +S+  S                       EPT+CPAMKDLCTNPAVS+MCKKTCA
Sbjct: 1147 EPTICPTMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTST--------EPTLCPAMKDLCTNPAVSIMCKKTCASTTTGISSASSGSSKSSTPLSTSSTSGSSVSSTPVSTEPTLCPAMKDLCTNPAVSIMCKKTCA 1282          

HSP 9 Score: 94.7449 bits (234), Expect = 3.587e-23
Identity = 90/135 (66.67%), Postives = 106/135 (78.52%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA-------------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPTVCPDMKDMCTNPSVAKLCK TC SSTTP+SSTSGSSKSSTSGSSISS P+STEPT+CP MKD+C NP+V+ +CK TCA              S+++ S  S+   TEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  458 EPTVCPDMKDMCTNPSVAKLCKKTCGSSTTPRSSTSGSSKSSTSGSSISSKPMSTEPTVCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTSESSKSSTSGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCA 592          

HSP 10 Score: 94.3597 bits (233), Expect = 5.491e-23
Identity = 95/136 (69.85%), Postives = 107/136 (78.68%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX--------------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPTVCPDMKDMCTNPSVAKLCK TCA  TTPKSSTSGSSKSSTSGSSISSTPISTEPT+CP MKD+C NP+V+ +CKKTC SST+  S +S                MSTEPT+CP MKD+CTNP+V+ +CK TCA
Sbjct:  403 EPTVCPDMKDMCTNPSVAKLCKKTCAFPTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCGSSTTPRSSTSGSSKSSTSGSSISSKPMSTEPTVCPDMKDMCTNPSVAKLCKNTCA 538          

HSP 11 Score: 88.9669 bits (219), Expect = 4.065e-21
Identity = 74/128 (57.81%), Postives = 95/128 (74.22%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPT CP+MKD+C NP+VA +CK TC S+T  KSSTSGS+ +ST  S+        EP+LCP++KDLC+NP+VS +CKKTC SST+A S  S        +STEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  309 EPTQCPNMKDLCINPTVASMCKKTCGSTTPSKSSTSGSTATSTPIST--------EPSLCPKLKDLCLNPSVSGVCKKTCGSSTTAKSSVSASSITSKPVSTEPTVCPDMKDMCTNPSVAKLCKKTCA 428          

HSP 12 Score: 72.7886 bits (177), Expect = 1.811e-15
Identity = 63/127 (49.61%), Postives = 87/127 (68.50%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX-----XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPT+CP MKD+C+  +V+ LCK TC SST+ KSSTS ++ S+T  S+        EP++CP MKD+C  P +S++CKKTCA+S+   S S+       + TEPT CP MK+ C+NP V++MCKKTC 
Sbjct:   94 EPTICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLST--------EPSVCPAMKDICNLPTISVMCKKTCATSSIGKSSSTFSISTTPIFTEPTQCPDMKNYCSNPGVALMCKKTCG 212          

HSP 13 Score: 68.9366 bits (167), Expect = 3.503e-14
Identity = 60/94 (63.83%), Postives = 72/94 (76.60%), Query Frame = 2
Query:  167 EPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPT CP MKD+C   +VS+ICKKTCASST  SS S T +STEPT CP MKDLC NP V+ MCKKTC S+T  KSS++ ++ +S P STEP+LCP
Sbjct:  268 EPTQCPLMKDICHLQSVSIICKKTCASSTPMSSKSPTPISTEPTQCPNMKDLCINPTVASMCKKTCGSTTPSKSSTSGSTATSTPISTEPSLCP 361          

HSP 14 Score: 67.3958 bits (163), Expect = 1.338e-13
Identity = 57/128 (44.53%), Postives = 73/128 (57.03%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EP  C  M  +C NP V+++C  TCAS+T   + +                    EPT+CP MKD+C    VS +CKKTC SSTS  S +S        +STEP++CPAMKD+C  P +SVMCKKTCA
Sbjct:   57 EPAQCAFMTQICHNPGVSQICPKTCASNTFTSTESPT------------------EPTICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLSTEPSVCPAMKDICNLPTISVMCKKTCA 166          

HSP 15 Score: 55.0694 bits (131), Expect = 2.271e-9
Identity = 51/95 (53.68%), Postives = 66/95 (69.47%), Query Frame = 2
Query:  167 EPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCPA 451
            EP  C  M  +C NP VS IC KTCA    +++ +ST   TEPTICP MKD+C+   VS +CKKTC SST+ KSS++ T+ S+ P STEP++CPA
Sbjct:   57 EPAQCAFMTQICHNPGVSQICPKTCA----SNTFTSTESPTEPTICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLSTEPSVCPA 147          
BLAST of SMED30024203 vs. Planmine SMEST
Match: SMESG000066354.1 (SMESG000066354.1)

HSP 1 Score: 114.775 bits (286), Expect = 3.630e-30
Identity = 123/150 (82.00%), Postives = 129/150 (86.00%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCPA 451
            EPT+CP MKD+CTNP+VA +CK TC SST+   ST  SS SST  S+        EPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMSTEPTICP MKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLCPA
Sbjct: 1062 EPTLCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPIST--------EPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMSTEPTICPTMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLCPA 1203          

HSP 2 Score: 102.834 bits (255), Expect = 4.834e-26
Identity = 99/155 (63.87%), Postives = 115/155 (74.19%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPTVCPDMKDMC NPS+AKLCK TCASST  KSSTSGSS SST  S+        EPT+CP MKD+C NP+V+ +CKKTC S+T+  S ++         STEPT+CPAMKDLCTNPAV+VMCKKTC SST+   S+  +S SS P STEPTLCP
Sbjct:  968 EPTVCPDMKDMCINPSIAKLCKKTCASSTKSKSSTSGSSVSSTPTST--------EPTVCPDMKDMCTNPSVAKLCKKTCGSTTTGKSSTNVSSVSSTPKSTEPTLCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPISTEPTLCP 1114          

HSP 3 Score: 101.293 bits (251), Expect = 1.735e-25
Identity = 104/156 (66.67%), Postives = 122/156 (78.21%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCPA 451
            EPTVCPDMKDMCTNPSVAKLCK TCA STTPKSS+ GSS SST  S+        EPT+CP MKD+C+NP+++ +CKKTCA      SSTS SS+SST  STEPT+CP MKD+CTNP+V+ +CKKTC S+TT KSS+  +S SS P STEPTLCPA
Sbjct:  921 EPTVCPDMKDMCTNPSVAKLCKKTCAFSTTPKSSSRGSSISSTQIST--------EPTVCPDMKDMCINPSIAKLCKKTCASSTKSKSSTSGSSVSSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCGSTTTGKSSTNVSSVSSTPKSTEPTLCPA 1068          

HSP 4 Score: 98.5969 bits (244), Expect = 1.531e-24
Identity = 110/162 (67.90%), Postives = 130/162 (80.25%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA-------------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPTVCPDMKDMCTNPSVAKLCK TCASSTTPKSSTSGSSKSSTSG SISSTPISTEPT+CP MKD+C NP+V+ +CKKTCA              S+++ S  S+   TEPT+CP MKD+CTNP+V+ +CKKTCASSTT +SS++ +S+SS   STEPT+CP
Sbjct:  570 EPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGPSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCASSTTQESSTSGSSKSSTSMSTEPTVCP 731          

HSP 5 Score: 97.4413 bits (241), Expect = 3.838e-24
Identity = 96/163 (58.90%), Postives = 115/163 (70.55%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA--------------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPTVCPDMKDMCTNPSVAKLCKNTCA         S ++  S++  S  S+   TEPT+CP MKD+C NP+V+ +CKKTCA              SSTS SS+SST +STEPT+CP MKD+CTNP+V+ +CKKTCA STT KSSS  +S SS   STEPT+CP
Sbjct:  820 EPTVCPDMKDMCTNPSVAKLCKNTCA---------SSTTPKSSTKGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCAFSTTPKSSSRGSSISSTQISTEPTVCP 973          

HSP 6 Score: 95.5153 bits (236), Expect = 1.753e-23
Identity = 74/128 (57.81%), Postives = 89/128 (69.53%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPT+CP MKD+C NP+V+ +CK TCASST+  S +S    +              EPT+CP MKDLC NPAVS++CKKTCASST+  S S+         STEPT+CPAMKDLCTNPAVS+MCKKTCA
Sbjct: 1109 EPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMST--------------EPTICPTMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLCPAMKDLCTNPAVSIMCKKTCA 1222          

HSP 7 Score: 95.1301 bits (235), Expect = 2.508e-23
Identity = 99/155 (63.87%), Postives = 120/155 (77.42%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX------EPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPTVCPDMKDMCTNPSVAKLCK TCASSTT +SSTSGSSKSSTS S+        EPT+CP MK++C NP+V+ +CKKTCAS+T+  S +S    +      EPT+CP MKD+CTNP+V+ +CKKTCASSTT KSS++ +S SS   STEPT+CP
Sbjct:  679 EPTVCPDMKDMCTNPSVAKLCKKTCASSTTQESSTSGSSKSSTSMST--------EPTVCPDMKEMCTNPSVAKLCKKTCASTTTPKSSTSGSSISSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCASSTTSKSSTSGSSISSTQISTEPTVCP 825          

HSP 8 Score: 94.7449 bits (234), Expect = 3.623e-23
Identity = 90/135 (66.67%), Postives = 106/135 (78.52%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA-------------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPTVCPDMKDMCTNPSVAKLCK TC SSTTP+SSTSGSSKSSTSGSSISS P+STEPT+CP MKD+C NP+V+ +CK TCA              S+++ S  S+   TEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  461 EPTVCPDMKDMCTNPSVAKLCKKTCGSSTTPRSSTSGSSKSSTSGSSISSKPMSTEPTVCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTSESSKSSTSGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCA 595          

HSP 9 Score: 94.7449 bits (234), Expect = 3.658e-23
Identity = 80/144 (55.56%), Postives = 98/144 (68.06%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX----------------------EPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPT+CP MKD+CTNP+V+ +CK TCASSTT KSS++ +S+SS   S+        EPTLCP MKDLC NPAVS++CKKTCAS+T+  S +S+  S                       EPT+CPAMKDLCTNPAVS+MCKKTCA
Sbjct: 1150 EPTICPTMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTST--------EPTLCPAMKDLCTNPAVSIMCKKTCASTTTGISSASSGSSKSSTPLSTSSTSGSSVSSTPVSTEPTLCPAMKDLCTNPAVSIMCKKTCA 1285          

HSP 10 Score: 94.3597 bits (233), Expect = 5.545e-23
Identity = 95/136 (69.85%), Postives = 107/136 (78.68%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX--------------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPTVCPDMKDMCTNPSVAKLCK TCA  TTPKSSTSGSSKSSTSGSSISSTPISTEPT+CP MKD+C NP+V+ +CKKTC SST+  S +S                MSTEPT+CP MKD+CTNP+V+ +CK TCA
Sbjct:  406 EPTVCPDMKDMCTNPSVAKLCKKTCAFPTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCGSSTTPRSSTSGSSKSSTSGSSISSKPMSTEPTVCPDMKDMCTNPSVAKLCKNTCA 541          

HSP 11 Score: 88.9669 bits (219), Expect = 3.988e-21
Identity = 74/128 (57.81%), Postives = 95/128 (74.22%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPT CP+MKD+C NP+VA +CK TC S+T  KSSTSGS+ +ST  S+        EP+LCP++KDLC+NP+VS +CKKTC SST+A S  S        +STEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  312 EPTQCPNMKDLCINPTVASMCKKTCGSTTPSKSSTSGSTATSTPIST--------EPSLCPKLKDLCLNPSVSGVCKKTCGSSTTAKSSVSASSITSKPVSTEPTVCPDMKDMCTNPSVAKLCKKTCA 431          

HSP 12 Score: 72.7886 bits (177), Expect = 1.846e-15
Identity = 63/127 (49.61%), Postives = 87/127 (68.50%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX-----XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPT+CP MKD+C+  +V+ LCK TC SST+ KSSTS ++ S+T  S+        EP++CP MKD+C  P +S++CKKTCA+S+   S S+       + TEPT CP MK+ C+NP V++MCKKTC 
Sbjct:   97 EPTICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLST--------EPSVCPAMKDICNLPTISVMCKKTCATSSIGKSSSTFSISTTPIFTEPTQCPDMKNYCSNPGVALMCKKTCG 215          

HSP 13 Score: 68.9366 bits (167), Expect = 3.571e-14
Identity = 60/94 (63.83%), Postives = 72/94 (76.60%), Query Frame = 2
Query:  167 EPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPT CP MKD+C   +VS+ICKKTCASST  SS S T +STEPT CP MKDLC NP V+ MCKKTC S+T  KSS++ ++ +S P STEP+LCP
Sbjct:  271 EPTQCPLMKDICHLQSVSIICKKTCASSTPMSSKSPTPISTEPTQCPNMKDLCINPTVASMCKKTCGSTTPSKSSTSGSTATSTPISTEPSLCP 364          

HSP 14 Score: 67.3958 bits (163), Expect = 1.339e-13
Identity = 57/128 (44.53%), Postives = 73/128 (57.03%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EP  C  M  +C NP V+++C  TCAS+T   + +                    EPT+CP MKD+C    VS +CKKTC SSTS  S +S        +STEP++CPAMKD+C  P +SVMCKKTCA
Sbjct:   60 EPAQCAFMTQICHNPGVSQICPKTCASNTFTSTESPT------------------EPTICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLSTEPSVCPAMKDICNLPTISVMCKKTCA 169          

HSP 15 Score: 55.0694 bits (131), Expect = 2.207e-9
Identity = 51/95 (53.68%), Postives = 66/95 (69.47%), Query Frame = 2
Query:  167 EPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCPA 451
            EP  C  M  +C NP VS IC KTCA    +++ +ST   TEPTICP MKD+C+   VS +CKKTC SST+ KSS++ T+ S+ P STEP++CPA
Sbjct:   60 EPAQCAFMTQICHNPGVSQICPKTCA----SNTFTSTESPTEPTICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLSTEPSVCPA 150          
BLAST of SMED30024203 vs. Planmine SMEST
Match: SMESG000066354.1 (SMESG000066354.1)

HSP 1 Score: 114.775 bits (286), Expect = 3.846e-30
Identity = 123/150 (82.00%), Postives = 129/150 (86.00%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCPA 451
            EPT+CP MKD+CTNP+VA +CK TC SST+   ST  SS SST  S+        EPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMSTEPTICP MKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLCPA
Sbjct: 1060 EPTLCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPIST--------EPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMSTEPTICPTMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLCPA 1201          

HSP 2 Score: 102.834 bits (255), Expect = 5.073e-26
Identity = 99/155 (63.87%), Postives = 115/155 (74.19%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPTVCPDMKDMC NPS+AKLCK TCASST  KSSTSGSS SST  S+        EPT+CP MKD+C NP+V+ +CKKTC S+T+  S ++         STEPT+CPAMKDLCTNPAV+VMCKKTC SST+   S+  +S SS P STEPTLCP
Sbjct:  966 EPTVCPDMKDMCINPSIAKLCKKTCASSTKSKSSTSGSSVSSTPTST--------EPTVCPDMKDMCTNPSVAKLCKKTCGSTTTGKSSTNVSSVSSTPKSTEPTLCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPISTEPTLCP 1112          

HSP 3 Score: 101.293 bits (251), Expect = 1.856e-25
Identity = 104/156 (66.67%), Postives = 122/156 (78.21%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCPA 451
            EPTVCPDMKDMCTNPSVAKLCK TCA STTPKSS+ GSS SST  S+        EPT+CP MKD+C+NP+++ +CKKTCA      SSTS SS+SST  STEPT+CP MKD+CTNP+V+ +CKKTC S+TT KSS+  +S SS P STEPTLCPA
Sbjct:  919 EPTVCPDMKDMCTNPSVAKLCKKTCAFSTTPKSSSRGSSISSTQIST--------EPTVCPDMKDMCINPSIAKLCKKTCASSTKSKSSTSGSSVSSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCGSTTTGKSSTNVSSVSSTPKSTEPTLCPA 1066          

HSP 4 Score: 97.8265 bits (242), Expect = 2.735e-24
Identity = 117/163 (71.78%), Postives = 135/163 (82.82%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA--------------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPTVCPDMKDMCTNPSVAKLCK TCASSTTPKSSTSGSSKSSTSG SISSTPISTEPT+CP MKD+C NP+V+ +CKKTCA              SSTS SS+SST +STEPT+CP MKD+CTNP+V+ +CKKTCASSTT +SS++ +S+SS   STEPT+CP
Sbjct:  567 EPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGPSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCASSTTQESSTSGSSKSSTSMSTEPTVCP 729          

HSP 5 Score: 97.4413 bits (241), Expect = 4.146e-24
Identity = 96/163 (58.90%), Postives = 115/163 (70.55%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA--------------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPTVCPDMKDMCTNPSVAKLCKNTCA         S ++  S++  S  S+   TEPT+CP MKD+C NP+V+ +CKKTCA              SSTS SS+SST +STEPT+CP MKD+CTNP+V+ +CKKTCA STT KSSS  +S SS   STEPT+CP
Sbjct:  818 EPTVCPDMKDMCTNPSVAKLCKNTCA---------SSTTPKSSTKGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCAFSTTPKSSSRGSSISSTQISTEPTVCP 971          

HSP 6 Score: 95.5153 bits (236), Expect = 1.858e-23
Identity = 74/128 (57.81%), Postives = 89/128 (69.53%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPT+CP MKD+C NP+V+ +CK TCASST+  S +S    +              EPT+CP MKDLC NPAVS++CKKTCASST+  S S+         STEPT+CPAMKDLCTNPAVS+MCKKTCA
Sbjct: 1107 EPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMST--------------EPTICPTMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLCPAMKDLCTNPAVSIMCKKTCA 1220          

HSP 7 Score: 95.1301 bits (235), Expect = 2.710e-23
Identity = 99/155 (63.87%), Postives = 120/155 (77.42%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX------EPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPTVCPDMKDMCTNPSVAKLCK TCASSTT +SSTSGSSKSSTS S+        EPT+CP MK++C NP+V+ +CKKTCAS+T+  S +S    +      EPT+CP MKD+CTNP+V+ +CKKTCASSTT KSS++ +S SS   STEPT+CP
Sbjct:  677 EPTVCPDMKDMCTNPSVAKLCKKTCASSTTQESSTSGSSKSSTSMST--------EPTVCPDMKEMCTNPSVAKLCKKTCASTTTPKSSTSGSSISSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCASSTTSKSSTSGSSISSTQISTEPTVCP 823          

HSP 8 Score: 94.7449 bits (234), Expect = 3.876e-23
Identity = 80/144 (55.56%), Postives = 98/144 (68.06%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX----------------------EPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPT+CP MKD+CTNP+V+ +CK TCASSTT KSS++ +S+SS   S+        EPTLCP MKDLC NPAVS++CKKTCAS+T+  S +S+  S                       EPT+CPAMKDLCTNPAVS+MCKKTCA
Sbjct: 1148 EPTICPTMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTST--------EPTLCPAMKDLCTNPAVSIMCKKTCASTTTGISSASSGSSKSSTPLSTSSTSGSSVSSTPVSTEPTLCPAMKDLCTNPAVSIMCKKTCA 1283          

HSP 9 Score: 94.7449 bits (234), Expect = 3.914e-23
Identity = 90/135 (66.67%), Postives = 106/135 (78.52%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA-------------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPTVCPDMKDMCTNPSVAKLCK TC SSTTP+SSTSGSSKSSTSGSSISS P+STEPT+CP MKD+C NP+V+ +CK TCA              S+++ S  S+   TEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  458 EPTVCPDMKDMCTNPSVAKLCKKTCGSSTTPRSSTSGSSKSSTSGSSISSKPMSTEPTVCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTSESSKSSTSGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCA 592          

HSP 10 Score: 93.9745 bits (232), Expect = 5.991e-23
Identity = 95/136 (69.85%), Postives = 107/136 (78.68%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX--------------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPTVCPDMKDMCTNPSVAKLCK TCA  TTPKSSTSGSSKSSTSGSSISSTPISTEPT+CP MKD+C NP+V+ +CKKTC SST+  S +S                MSTEPT+CP MKD+CTNP+V+ +CK TCA
Sbjct:  403 EPTVCPDMKDMCTNPSVAKLCKKTCAFPTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCGSSTTPRSSTSGSSKSSTSGSSISSKPMSTEPTVCPDMKDMCTNPSVAKLCKNTCA 538          

HSP 11 Score: 88.5817 bits (218), Expect = 4.435e-21
Identity = 74/128 (57.81%), Postives = 95/128 (74.22%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPT CP+MKD+C NP+VA +CK TC S+T  KSSTSGS+ +ST  S+        EP+LCP++KDLC+NP+VS +CKKTC SST+A S  S        +STEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  309 EPTQCPNMKDLCINPTVASMCKKTCGSTTPSKSSTSGSTATSTPIST--------EPSLCPKLKDLCLNPSVSGVCKKTCGSSTTAKSSVSASSITSKPVSTEPTVCPDMKDMCTNPSVAKLCKKTCA 428          

HSP 12 Score: 72.4034 bits (176), Expect = 1.975e-15
Identity = 63/127 (49.61%), Postives = 87/127 (68.50%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX-----XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPT+CP MKD+C+  +V+ LCK TC SST+ KSSTS ++ S+T  S+        EP++CP MKD+C  P +S++CKKTCA+S+   S S+       + TEPT CP MK+ C+NP V++MCKKTC 
Sbjct:   94 EPTICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLST--------EPSVCPAMKDICNLPTISVMCKKTCATSSIGKSSSTFSISTTPIFTEPTQCPDMKNYCSNPGVALMCKKTCG 212          

HSP 13 Score: 68.9366 bits (167), Expect = 3.784e-14
Identity = 60/94 (63.83%), Postives = 72/94 (76.60%), Query Frame = 2
Query:  167 EPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPT CP MKD+C   +VS+ICKKTCASST  SS S T +STEPT CP MKDLC NP V+ MCKKTC S+T  KSS++ ++ +S P STEP+LCP
Sbjct:  268 EPTQCPLMKDICHLQSVSIICKKTCASSTPMSSKSPTPISTEPTQCPNMKDLCINPTVASMCKKTCGSTTPSKSSTSGSTATSTPISTEPSLCP 361          

HSP 14 Score: 67.0106 bits (162), Expect = 1.460e-13
Identity = 57/128 (44.53%), Postives = 73/128 (57.03%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EP  C  M  +C NP V+++C  TCAS+T   + +                    EPT+CP MKD+C    VS +CKKTC SSTS  S +S        +STEP++CPAMKD+C  P +SVMCKKTCA
Sbjct:   57 EPAQCAFMTQICHNPGVSQICPKTCASNTFTSTESPT------------------EPTICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLSTEPSVCPAMKDICNLPTISVMCKKTCA 166          

HSP 15 Score: 55.0694 bits (131), Expect = 2.429e-9
Identity = 51/95 (53.68%), Postives = 66/95 (69.47%), Query Frame = 2
Query:  167 EPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCPA 451
            EP  C  M  +C NP VS IC KTCA    +++ +ST   TEPTICP MKD+C+   VS +CKKTC SST+ KSS++ T+ S+ P STEP++CPA
Sbjct:   57 EPAQCAFMTQICHNPGVSQICPKTCA----SNTFTSTESPTEPTICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLSTEPSVCPA 147          
BLAST of SMED30024203 vs. Planmine SMEST
Match: SMESG000066354.1 (SMESG000066354.1)

HSP 1 Score: 114.775 bits (286), Expect = 3.922e-30
Identity = 123/150 (82.00%), Postives = 129/150 (86.00%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCPA 451
            EPT+CP MKD+CTNP+VA +CK TC SST+   ST  SS SST  S+        EPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMSTEPTICP MKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLCPA
Sbjct: 1063 EPTLCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPIST--------EPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMSTEPTICPTMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLCPA 1204          

HSP 2 Score: 102.834 bits (255), Expect = 5.275e-26
Identity = 99/155 (63.87%), Postives = 115/155 (74.19%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPTVCPDMKDMC NPS+AKLCK TCASST  KSSTSGSS SST  S+        EPT+CP MKD+C NP+V+ +CKKTC S+T+  S ++         STEPT+CPAMKDLCTNPAV+VMCKKTC SST+   S+  +S SS P STEPTLCP
Sbjct:  969 EPTVCPDMKDMCINPSIAKLCKKTCASSTKSKSSTSGSSVSSTPTST--------EPTVCPDMKDMCTNPSVAKLCKKTCGSTTTGKSSTNVSSVSSTPKSTEPTLCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPISTEPTLCP 1115          

HSP 3 Score: 101.293 bits (251), Expect = 1.892e-25
Identity = 104/156 (66.67%), Postives = 122/156 (78.21%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCPA 451
            EPTVCPDMKDMCTNPSVAKLCK TCA STTPKSS+ GSS SST  S+        EPT+CP MKD+C+NP+++ +CKKTCA      SSTS SS+SST  STEPT+CP MKD+CTNP+V+ +CKKTC S+TT KSS+  +S SS P STEPTLCPA
Sbjct:  922 EPTVCPDMKDMCTNPSVAKLCKKTCAFSTTPKSSSRGSSISSTQIST--------EPTVCPDMKDMCINPSIAKLCKKTCASSTKSKSSTSGSSVSSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCGSTTTGKSSTNVSSVSSTPKSTEPTLCPA 1069          

HSP 4 Score: 97.8265 bits (242), Expect = 2.762e-24
Identity = 117/163 (71.78%), Postives = 135/163 (82.82%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA--------------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPTVCPDMKDMCTNPSVAKLCK TCASSTTPKSSTSGSSKSSTSG SISSTPISTEPT+CP MKD+C NP+V+ +CKKTCA              SSTS SS+SST +STEPT+CP MKD+CTNP+V+ +CKKTCASSTT +SS++ +S+SS   STEPT+CP
Sbjct:  570 EPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGPSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCASSTTQESSTSGSSKSSTSMSTEPTVCP 732          

HSP 5 Score: 97.4413 bits (241), Expect = 4.187e-24
Identity = 96/163 (58.90%), Postives = 115/163 (70.55%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA--------------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPTVCPDMKDMCTNPSVAKLCKNTCA         S ++  S++  S  S+   TEPT+CP MKD+C NP+V+ +CKKTCA              SSTS SS+SST +STEPT+CP MKD+CTNP+V+ +CKKTCA STT KSSS  +S SS   STEPT+CP
Sbjct:  821 EPTVCPDMKDMCTNPSVAKLCKNTCA---------SSTTPKSSTKGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCAFSTTPKSSSRGSSISSTQISTEPTVCP 974          

HSP 6 Score: 95.5153 bits (236), Expect = 1.895e-23
Identity = 74/128 (57.81%), Postives = 89/128 (69.53%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPT+CP MKD+C NP+V+ +CK TCASST+  S +S    +              EPT+CP MKDLC NPAVS++CKKTCASST+  S S+         STEPT+CPAMKDLCTNPAVS+MCKKTCA
Sbjct: 1110 EPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMST--------------EPTICPTMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLCPAMKDLCTNPAVSIMCKKTCA 1223          

HSP 7 Score: 95.1301 bits (235), Expect = 2.736e-23
Identity = 99/155 (63.87%), Postives = 120/155 (77.42%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX------EPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPTVCPDMKDMCTNPSVAKLCK TCASSTT +SSTSGSSKSSTS S+        EPT+CP MK++C NP+V+ +CKKTCAS+T+  S +S    +      EPT+CP MKD+CTNP+V+ +CKKTCASSTT KSS++ +S SS   STEPT+CP
Sbjct:  680 EPTVCPDMKDMCTNPSVAKLCKKTCASSTTQESSTSGSSKSSTSMST--------EPTVCPDMKEMCTNPSVAKLCKKTCASTTTPKSSTSGSSISSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCASSTTSKSSTSGSSISSTQISTEPTVCP 826          

HSP 8 Score: 94.7449 bits (234), Expect = 3.914e-23
Identity = 90/135 (66.67%), Postives = 106/135 (78.52%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA-------------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPTVCPDMKDMCTNPSVAKLCK TC SSTTP+SSTSGSSKSSTSGSSISS P+STEPT+CP MKD+C NP+V+ +CK TCA              S+++ S  S+   TEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  461 EPTVCPDMKDMCTNPSVAKLCKKTCGSSTTPRSSTSGSSKSSTSGSSISSKPMSTEPTVCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTSESSKSSTSGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCA 595          

HSP 9 Score: 94.7449 bits (234), Expect = 3.952e-23
Identity = 80/144 (55.56%), Postives = 98/144 (68.06%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX----------------------EPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPT+CP MKD+CTNP+V+ +CK TCASSTT KSS++ +S+SS   S+        EPTLCP MKDLC NPAVS++CKKTCAS+T+  S +S+  S                       EPT+CPAMKDLCTNPAVS+MCKKTCA
Sbjct: 1151 EPTICPTMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTST--------EPTLCPAMKDLCTNPAVSIMCKKTCASTTTGISSASSGSSKSSTPLSTSSTSGSSVSSTPVSTEPTLCPAMKDLCTNPAVSIMCKKTCA 1286          

HSP 10 Score: 93.9745 bits (232), Expect = 5.992e-23
Identity = 95/136 (69.85%), Postives = 107/136 (78.68%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX--------------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPTVCPDMKDMCTNPSVAKLCK TCA  TTPKSSTSGSSKSSTSGSSISSTPISTEPT+CP MKD+C NP+V+ +CKKTC SST+  S +S                MSTEPT+CP MKD+CTNP+V+ +CK TCA
Sbjct:  406 EPTVCPDMKDMCTNPSVAKLCKKTCAFPTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCGSSTTPRSSTSGSSKSSTSGSSISSKPMSTEPTVCPDMKDMCTNPSVAKLCKNTCA 541          

HSP 11 Score: 88.9669 bits (219), Expect = 4.309e-21
Identity = 74/128 (57.81%), Postives = 95/128 (74.22%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPT CP+MKD+C NP+VA +CK TC S+T  KSSTSGS+ +ST  S+        EP+LCP++KDLC+NP+VS +CKKTC SST+A S  S        +STEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  312 EPTQCPNMKDLCINPTVASMCKKTCGSTTPSKSSTSGSTATSTPIST--------EPSLCPKLKDLCLNPSVSGVCKKTCGSSTTAKSSVSASSITSKPVSTEPTVCPDMKDMCTNPSVAKLCKKTCA 431          

HSP 12 Score: 72.4034 bits (176), Expect = 2.014e-15
Identity = 63/127 (49.61%), Postives = 87/127 (68.50%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX-----XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPT+CP MKD+C+  +V+ LCK TC SST+ KSSTS ++ S+T  S+        EP++CP MKD+C  P +S++CKKTCA+S+   S S+       + TEPT CP MK+ C+NP V++MCKKTC 
Sbjct:   97 EPTICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLST--------EPSVCPAMKDICNLPTISVMCKKTCATSSIGKSSSTFSISTTPIFTEPTQCPDMKNYCSNPGVALMCKKTCG 215          

HSP 13 Score: 68.9366 bits (167), Expect = 3.784e-14
Identity = 60/94 (63.83%), Postives = 72/94 (76.60%), Query Frame = 2
Query:  167 EPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPT CP MKD+C   +VS+ICKKTCASST  SS S T +STEPT CP MKDLC NP V+ MCKKTC S+T  KSS++ ++ +S P STEP+LCP
Sbjct:  271 EPTQCPLMKDICHLQSVSIICKKTCASSTPMSSKSPTPISTEPTQCPNMKDLCINPTVASMCKKTCGSTTPSKSSTSGSTATSTPISTEPSLCP 364          

HSP 14 Score: 67.3958 bits (163), Expect = 1.405e-13
Identity = 57/128 (44.53%), Postives = 73/128 (57.03%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EP  C  M  +C NP V+++C  TCAS+T   + +                    EPT+CP MKD+C    VS +CKKTC SSTS  S +S        +STEP++CPAMKD+C  P +SVMCKKTCA
Sbjct:   60 EPAQCAFMTQICHNPGVSQICPKTCASNTFTSTESPT------------------EPTICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLSTEPSVCPAMKDICNLPTISVMCKKTCA 169          

HSP 15 Score: 55.0694 bits (131), Expect = 2.272e-9
Identity = 51/95 (53.68%), Postives = 66/95 (69.47%), Query Frame = 2
Query:  167 EPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCPA 451
            EP  C  M  +C NP VS IC KTCA    +++ +ST   TEPTICP MKD+C+   VS +CKKTC SST+ KSS++ T+ S+ P STEP++CPA
Sbjct:   60 EPAQCAFMTQICHNPGVSQICPKTCA----SNTFTSTESPTEPTICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLSTEPSVCPA 150          
BLAST of SMED30024203 vs. Planmine SMEST
Match: SMESG000066354.1 (SMESG000066354.1)

HSP 1 Score: 114.775 bits (286), Expect = 4.192e-30
Identity = 123/150 (82.00%), Postives = 129/150 (86.00%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCPA 451
            EPT+CP MKD+CTNP+VA +CK TC SST+   ST  SS SST  S+        EPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMSTEPTICP MKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLCPA
Sbjct: 1060 EPTLCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPIST--------EPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMSTEPTICPTMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLCPA 1201          

HSP 2 Score: 102.834 bits (255), Expect = 5.476e-26
Identity = 99/155 (63.87%), Postives = 115/155 (74.19%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPTVCPDMKDMC NPS+AKLCK TCASST  KSSTSGSS SST  S+        EPT+CP MKD+C NP+V+ +CKKTC S+T+  S ++         STEPT+CPAMKDLCTNPAV+VMCKKTC SST+   S+  +S SS P STEPTLCP
Sbjct:  966 EPTVCPDMKDMCINPSIAKLCKKTCASSTKSKSSTSGSSVSSTPTST--------EPTVCPDMKDMCTNPSVAKLCKKTCGSTTTGKSSTNVSSVSSTPKSTEPTLCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPISTEPTLCP 1112          

HSP 3 Score: 101.293 bits (251), Expect = 1.946e-25
Identity = 104/156 (66.67%), Postives = 122/156 (78.21%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCPA 451
            EPTVCPDMKDMCTNPSVAKLCK TCA STTPKSS+ GSS SST  S+        EPT+CP MKD+C+NP+++ +CKKTCA      SSTS SS+SST  STEPT+CP MKD+CTNP+V+ +CKKTC S+TT KSS+  +S SS P STEPTLCPA
Sbjct:  919 EPTVCPDMKDMCTNPSVAKLCKKTCAFSTTPKSSSRGSSISSTQIST--------EPTVCPDMKDMCINPSIAKLCKKTCASSTKSKSSTSGSSVSSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCGSTTTGKSSTNVSSVSSTPKSTEPTLCPA 1066          

HSP 4 Score: 97.8265 bits (242), Expect = 2.981e-24
Identity = 117/163 (71.78%), Postives = 135/163 (82.82%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA--------------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPTVCPDMKDMCTNPSVAKLCK TCASSTTPKSSTSGSSKSSTSG SISSTPISTEPT+CP MKD+C NP+V+ +CKKTCA              SSTS SS+SST +STEPT+CP MKD+CTNP+V+ +CKKTCASSTT +SS++ +S+SS   STEPT+CP
Sbjct:  567 EPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGPSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCASSTTQESSTSGSSKSSTSMSTEPTVCP 729          

HSP 5 Score: 97.4413 bits (241), Expect = 4.519e-24
Identity = 96/163 (58.90%), Postives = 115/163 (70.55%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA--------------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPTVCPDMKDMCTNPSVAKLCKNTCA         S ++  S++  S  S+   TEPT+CP MKD+C NP+V+ +CKKTCA              SSTS SS+SST +STEPT+CP MKD+CTNP+V+ +CKKTCA STT KSSS  +S SS   STEPT+CP
Sbjct:  818 EPTVCPDMKDMCTNPSVAKLCKNTCA---------SSTTPKSSTKGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCAFSTTPKSSSRGSSISSTQISTEPTVCP 971          

HSP 6 Score: 95.5153 bits (236), Expect = 1.986e-23
Identity = 74/128 (57.81%), Postives = 89/128 (69.53%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPT+CP MKD+C NP+V+ +CK TCASST+  S +S    +              EPT+CP MKDLC NPAVS++CKKTCASST+  S S+         STEPT+CPAMKDLCTNPAVS+MCKKTCA
Sbjct: 1107 EPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMST--------------EPTICPTMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLCPAMKDLCTNPAVSIMCKKTCA 1220          

HSP 7 Score: 95.1301 bits (235), Expect = 2.953e-23
Identity = 99/155 (63.87%), Postives = 120/155 (77.42%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX------EPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPTVCPDMKDMCTNPSVAKLCK TCASSTT +SSTSGSSKSSTS S+        EPT+CP MK++C NP+V+ +CKKTCAS+T+  S +S    +      EPT+CP MKD+CTNP+V+ +CKKTCASSTT KSS++ +S SS   STEPT+CP
Sbjct:  677 EPTVCPDMKDMCTNPSVAKLCKKTCASSTTQESSTSGSSKSSTSMST--------EPTVCPDMKEMCTNPSVAKLCKKTCASTTTPKSSTSGSSISSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCASSTTSKSSTSGSSISSTQISTEPTVCP 823          

HSP 8 Score: 94.3597 bits (233), Expect = 4.224e-23
Identity = 90/135 (66.67%), Postives = 106/135 (78.52%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA-------------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPTVCPDMKDMCTNPSVAKLCK TC SSTTP+SSTSGSSKSSTSGSSISS P+STEPT+CP MKD+C NP+V+ +CK TCA              S+++ S  S+   TEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  458 EPTVCPDMKDMCTNPSVAKLCKKTCGSSTTPRSSTSGSSKSSTSGSSISSKPMSTEPTVCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTSESSKSSTSGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCA 592          

HSP 9 Score: 93.9745 bits (232), Expect = 6.466e-23
Identity = 95/136 (69.85%), Postives = 107/136 (78.68%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX--------------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPTVCPDMKDMCTNPSVAKLCK TCA  TTPKSSTSGSSKSSTSGSSISSTPISTEPT+CP MKD+C NP+V+ +CKKTC SST+  S +S                MSTEPT+CP MKD+CTNP+V+ +CK TCA
Sbjct:  403 EPTVCPDMKDMCTNPSVAKLCKKTCAFPTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCGSSTTPRSSTSGSSKSSTSGSSISSKPMSTEPTVCPDMKDMCTNPSVAKLCKNTCA 538          

HSP 10 Score: 88.5817 bits (218), Expect = 4.561e-21
Identity = 74/128 (57.81%), Postives = 95/128 (74.22%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPT CP+MKD+C NP+VA +CK TC S+T  KSSTSGS+ +ST  S+        EP+LCP++KDLC+NP+VS +CKKTC SST+A S  S        +STEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  309 EPTQCPNMKDLCINPTVASMCKKTCGSTTPSKSSTSGSTATSTPIST--------EPSLCPKLKDLCLNPSVSGVCKKTCGSSTTAKSSVSASSITSKPVSTEPTVCPDMKDMCTNPSVAKLCKKTCA 428          

HSP 11 Score: 88.5817 bits (218), Expect = 5.324e-21
Identity = 76/129 (58.91%), Postives = 95/129 (73.64%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX-------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPT+CP MKD+CTNP+V+ +CK TCASSTT KSS++ +S+SS   S+        EPTLCP MKDLC NPAVS++CKKTCAS+T+  S +S+        +STEPT+CP MK LC NP V+ MCKKTCA
Sbjct: 1148 EPTICPTMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTST--------EPTLCPAMKDLCTNPAVSIMCKKTCASTTTGISSASSGSSKSSTPLSTEPTLCPDMKMLCNNPNVAKMCKKTCA 1268          

HSP 12 Score: 72.4034 bits (176), Expect = 2.051e-15
Identity = 63/127 (49.61%), Postives = 87/127 (68.50%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX-----XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EPT+CP MKD+C+  +V+ LCK TC SST+ KSSTS ++ S+T  S+        EP++CP MKD+C  P +S++CKKTCA+S+   S S+       + TEPT CP MK+ C+NP V++MCKKTC 
Sbjct:   94 EPTICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLST--------EPSVCPAMKDICNLPTISVMCKKTCATSSIGKSSSTFSISTTPIFTEPTQCPDMKNYCSNPGVALMCKKTCG 212          

HSP 13 Score: 68.9366 bits (167), Expect = 3.816e-14
Identity = 60/94 (63.83%), Postives = 72/94 (76.60%), Query Frame = 2
Query:  167 EPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCP 448
            EPT CP MKD+C   +VS+ICKKTCASST  SS S T +STEPT CP MKDLC NP V+ MCKKTC S+T  KSS++ ++ +S P STEP+LCP
Sbjct:  268 EPTQCPLMKDICHLQSVSIICKKTCASSTPMSSKSPTPISTEPTQCPNMKDLCINPTVASMCKKTCGSTTPSKSSTSGSTATSTPISTEPSLCP 361          

HSP 14 Score: 67.0106 bits (162), Expect = 1.458e-13
Identity = 57/128 (44.53%), Postives = 73/128 (57.03%), Query Frame = 2
Query:    2 EPTVCPDMKDMCTNPSVAKLCKNTCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 367
            EP  C  M  +C NP V+++C  TCAS+T   + +                    EPT+CP MKD+C    VS +CKKTC SSTS  S +S        +STEP++CPAMKD+C  P +SVMCKKTCA
Sbjct:   57 EPAQCAFMTQICHNPGVSQICPKTCASNTFTSTESPT------------------EPTICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLSTEPSVCPAMKDICNLPTISVMCKKTCA 166          

HSP 15 Score: 55.0694 bits (131), Expect = 2.381e-9
Identity = 51/95 (53.68%), Postives = 66/95 (69.47%), Query Frame = 2
Query:  167 EPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLCPA 451
            EP  C  M  +C NP VS IC KTCA    +++ +ST   TEPTICP MKD+C+   VS +CKKTC SST+ KSS++ T+ S+ P STEP++CPA
Sbjct:   57 EPAQCAFMTQICHNPGVSQICPKTCA----SNTFTSTESPTEPTICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLSTEPSVCPA 147          
The following BLAST results are available for this feature:
BLAST of SMED30024203 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024203 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024203 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024203 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024203 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024203 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024203 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024203 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024203 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024203 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024203 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024203 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024203 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024203 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000066354.13.424e-3082.00SMESG000066354.1[more]
SMESG000066354.13.630e-3082.00SMESG000066354.1[more]
SMESG000066354.13.846e-3082.00SMESG000066354.1[more]
SMESG000066354.13.922e-3082.00SMESG000066354.1[more]
SMESG000066354.14.192e-3082.00SMESG000066354.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024203 ID=SMED30024203|Name=SMED30024203|organism=Schmidtea mediterranea sexual|type=transcript|length=451bp
CGAACCAACGGTTTGCCCTGACATGAAGGATATGTGTACCAACCCCAGTG
TAGCTAAACTTTGTAAGAATACATGTGCTTCTTCAACAACACCGAAGTCA
TCAACAAGTGGATCATCAAAATCTTCAACAAGCGGATCATCTATATCATC
AACACCTATAAGTACTGAGCCTACATTATGCCCGCAAATGAAAGATTTAT
GCATGAATCCAGCAGTGTCACTGATATGCAAAAAGACATGCGCTTCATCA
ACAAGTGCTTCATCTTTGTCCTCTACAATTATGAGTACTGAACCAACAAT
ATGTCCTGCAATGAAGGATCTATGCACAAATCCAGCTGTGTCAGTTATGT
GCAAAAAAACATGTGCCTCATCAACAACACTAAAATCATCCTCAACTGCC
ACTTCTCAGTCTTCTGTGCCAACTAGCACCGAGCCAACTTTATGTCCAGC
A
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protein sequence of SMED30024203-orf-1

>SMED30024203-orf-1 ID=SMED30024203-orf-1|Name=SMED30024203-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=150bp
EPTVCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTSGSSKSSTSGSSISS
TPISTEPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMSTEPTI
CPAMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLCPA
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InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 25..54
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 124..150