40S ribosomal protein S14

Overview
Name40S ribosomal protein S14
Smed IDSMED30024202
Length (bp)569
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of 40S ribosomal protein S14 (SMED30024202) t-SNE clustered cells

Violin plots show distribution of expression levels for 40S ribosomal protein S14 (SMED30024202) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of 40S ribosomal protein S14 (SMED30024202) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for 40S ribosomal protein S14 (SMED30024202) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30024202

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 13

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30024202SMESG000036550.1 dd_Smed_v4_139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30024202SMESG000036550.1 dd_Smed_v4_139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30024202SMESG000036550.1 dd_Smed_v4_139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30024202SMESG000036550.1 dd_Smed_v4_139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30024202SMESG000036550.1 dd_Smed_v4_139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30024202SMESG000036550.1 dd_Smed_v4_139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30024202SMESG000036550.1 dd_Smed_v4_139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30024202SMESG000036550.1 dd_Smed_v4_139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30024202SMESG000036550.1 dd_Smed_v4_139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30024202SMESG000036550.1 dd_Smed_v4_139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30024202SMESG000036550.1 dd_Smed_v4_139_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
progenitor cellSMED30024202SMESG000036550.1 dd_Smed_v6_139_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30024202SMESG000036550.1 dd_Smed_v6_139_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of 40S ribosomal protein S14 vs. Ensembl Human
Match: RPS14 (ribosomal protein S14 [Source:HGNC Symbol;Acc:HGNC:10387])

HSP 1 Score: 204.912 bits (520), Expect = 1.280e-67
Identity = 108/140 (77.14%), Postives = 125/140 (89.29%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA ++   KK E+ +SLGP+V EGE VFGVCHIFASFNDTFVHVTD++G ET  RVTGGMKVKADRDESSPYAAMLAAQDVA+RCK+LGITA+H+++RATGG +++TP P AQSALRALARSGMKIGRIEDVTPIP D+T
Sbjct:    1 MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDST 140          
BLAST of 40S ribosomal protein S14 vs. Ensembl Human
Match: RPS14 (ribosomal protein S14 [Source:HGNC Symbol;Acc:HGNC:10387])

HSP 1 Score: 204.912 bits (520), Expect = 1.280e-67
Identity = 108/140 (77.14%), Postives = 125/140 (89.29%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA ++   KK E+ +SLGP+V EGE VFGVCHIFASFNDTFVHVTD++G ET  RVTGGMKVKADRDESSPYAAMLAAQDVA+RCK+LGITA+H+++RATGG +++TP P AQSALRALARSGMKIGRIEDVTPIP D+T
Sbjct:    1 MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDST 140          
BLAST of 40S ribosomal protein S14 vs. Ensembl Human
Match: RPS14 (ribosomal protein S14 [Source:HGNC Symbol;Acc:HGNC:10387])

HSP 1 Score: 204.912 bits (520), Expect = 1.280e-67
Identity = 108/140 (77.14%), Postives = 125/140 (89.29%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA ++   KK E+ +SLGP+V EGE VFGVCHIFASFNDTFVHVTD++G ET  RVTGGMKVKADRDESSPYAAMLAAQDVA+RCK+LGITA+H+++RATGG +++TP P AQSALRALARSGMKIGRIEDVTPIP D+T
Sbjct:    1 MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDST 140          
BLAST of 40S ribosomal protein S14 vs. Ensembl Human
Match: RPS14 (ribosomal protein S14 [Source:HGNC Symbol;Acc:HGNC:10387])

HSP 1 Score: 204.912 bits (520), Expect = 1.280e-67
Identity = 108/140 (77.14%), Postives = 125/140 (89.29%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA ++   KK E+ +SLGP+V EGE VFGVCHIFASFNDTFVHVTD++G ET  RVTGGMKVKADRDESSPYAAMLAAQDVA+RCK+LGITA+H+++RATGG +++TP P AQSALRALARSGMKIGRIEDVTPIP D+T
Sbjct:    1 MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDST 140          
BLAST of 40S ribosomal protein S14 vs. Ensembl Human
Match: RPS14 (ribosomal protein S14 [Source:HGNC Symbol;Acc:HGNC:10387])

HSP 1 Score: 204.527 bits (519), Expect = 1.367e-67
Identity = 108/140 (77.14%), Postives = 125/140 (89.29%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA ++   KK E+ +SLGP+V EGE VFGVCHIFASFNDTFVHVTD++G ET  RVTGGMKVKADRDESSPYAAMLAAQDVA+RCK+LGITA+H+++RATGG +++TP P AQSALRALARSGMKIGRIEDVTPIP D+T
Sbjct:    1 MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDST 140          
BLAST of 40S ribosomal protein S14 vs. Ensembl Celegans
Match: rps-14 (40S ribosomal protein S14 [Source:UniProtKB/Swiss-Prot;Acc:P48150])

HSP 1 Score: 196.052 bits (497), Expect = 1.343e-64
Identity = 107/141 (75.89%), Postives = 122/141 (86.52%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQV-SLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA  RK   K E+ V SLGP+  EGE +FGV HIFASFNDTFVH+TD++G ET VRVTGGMKVKADRDESSPYAAMLAAQDVA+RCKQLGI A+H+++RATGGT+++TP P AQSALRALAR+GMKIGRIEDVTPIP D T
Sbjct:    1 MAPARKGKAKEEQAVVSLGPQAKEGELIFGVAHIFASFNDTFVHITDISGRETIVRVTGGMKVKADRDESSPYAAMLAAQDVADRCKQLGINALHIKLRATGGTRTKTPGPGAQSALRALARAGMKIGRIEDVTPIPSDCT 141          
BLAST of 40S ribosomal protein S14 vs. Ensembl Celegans
Match: rps-14 (40S ribosomal protein S14 [Source:UniProtKB/Swiss-Prot;Acc:P48150])

HSP 1 Score: 196.052 bits (497), Expect = 1.343e-64
Identity = 107/141 (75.89%), Postives = 122/141 (86.52%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQV-SLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA  RK   K E+ V SLGP+  EGE +FGV HIFASFNDTFVH+TD++G ET VRVTGGMKVKADRDESSPYAAMLAAQDVA+RCKQLGI A+H+++RATGGT+++TP P AQSALRALAR+GMKIGRIEDVTPIP D T
Sbjct:    1 MAPARKGKAKEEQAVVSLGPQAKEGELIFGVAHIFASFNDTFVHITDISGRETIVRVTGGMKVKADRDESSPYAAMLAAQDVADRCKQLGINALHIKLRATGGTRTKTPGPGAQSALRALARAGMKIGRIEDVTPIPSDCT 141          
BLAST of 40S ribosomal protein S14 vs. Ensembl Fly
Match: RpS14a (gene:FBgn0004403 transcript:FBtr0071095)

HSP 1 Score: 195.282 bits (495), Expect = 3.321e-64
Identity = 105/140 (75.00%), Postives = 122/140 (87.14%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA ++  V+K E QV LGP+V +GE VFGV HI+ASFNDTFVHVTD++G ET  RVTGGMKVKADRDE+SPYAAMLAAQDVAE+CK LGITA+H+++RATGG K++TP P AQSALRALARS MKIGRIEDVTPIP D+T
Sbjct:    1 MAPRKAKVQKEEVQVQLGPQVRDGEIVFGVAHIYASFNDTFVHVTDLSGRETIARVTGGMKVKADRDEASPYAAMLAAQDVAEKCKTLGITALHIKLRATGGNKTKTPGPGAQSALRALARSSMKIGRIEDVTPIPSDST 140          
BLAST of 40S ribosomal protein S14 vs. Ensembl Fly
Match: RpS14a (gene:FBgn0004403 transcript:FBtr0071094)

HSP 1 Score: 195.282 bits (495), Expect = 3.321e-64
Identity = 105/140 (75.00%), Postives = 122/140 (87.14%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA ++  V+K E QV LGP+V +GE VFGV HI+ASFNDTFVHVTD++G ET  RVTGGMKVKADRDE+SPYAAMLAAQDVAE+CK LGITA+H+++RATGG K++TP P AQSALRALARS MKIGRIEDVTPIP D+T
Sbjct:    1 MAPRKAKVQKEEVQVQLGPQVRDGEIVFGVAHIYASFNDTFVHVTDLSGRETIARVTGGMKVKADRDEASPYAAMLAAQDVAEKCKTLGITALHIKLRATGGNKTKTPGPGAQSALRALARSSMKIGRIEDVTPIPSDST 140          
BLAST of 40S ribosomal protein S14 vs. Ensembl Fly
Match: RpS14b (gene:FBgn0004404 transcript:FBtr0071096)

HSP 1 Score: 195.282 bits (495), Expect = 3.321e-64
Identity = 105/140 (75.00%), Postives = 122/140 (87.14%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA ++  V+K E QV LGP+V +GE VFGV HI+ASFNDTFVHVTD++G ET  RVTGGMKVKADRDE+SPYAAMLAAQDVAE+CK LGITA+H+++RATGG K++TP P AQSALRALARS MKIGRIEDVTPIP D+T
Sbjct:    1 MAPRKAKVQKEEVQVQLGPQVRDGEIVFGVAHIYASFNDTFVHVTDLSGRETIARVTGGMKVKADRDEASPYAAMLAAQDVAEKCKTLGITALHIKLRATGGNKTKTPGPGAQSALRALARSSMKIGRIEDVTPIPSDST 140          
BLAST of 40S ribosomal protein S14 vs. Ensembl Fly
Match: RpS14b (gene:FBgn0004404 transcript:FBtr0345291)

HSP 1 Score: 195.282 bits (495), Expect = 3.321e-64
Identity = 105/140 (75.00%), Postives = 122/140 (87.14%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA ++  V+K E QV LGP+V +GE VFGV HI+ASFNDTFVHVTD++G ET  RVTGGMKVKADRDE+SPYAAMLAAQDVAE+CK LGITA+H+++RATGG K++TP P AQSALRALARS MKIGRIEDVTPIP D+T
Sbjct:    1 MAPRKAKVQKEEVQVQLGPQVRDGEIVFGVAHIYASFNDTFVHVTDLSGRETIARVTGGMKVKADRDEASPYAAMLAAQDVAEKCKTLGITALHIKLRATGGNKTKTPGPGAQSALRALARSSMKIGRIEDVTPIPSDST 140          
BLAST of 40S ribosomal protein S14 vs. Ensembl Zebrafish
Match: rps14 (ribosomal protein S14 [Source:ZFIN;Acc:ZDB-GENE-030131-8631])

HSP 1 Score: 203.371 bits (516), Expect = 2.772e-67
Identity = 107/140 (76.43%), Postives = 125/140 (89.29%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA ++   KK E+ +SLGP+V EGE VFGVCHIFASFNDTFVHVTD++G ET  RVTGGMKVKADRDESSPYAAMLAAQDVA++CK+LGITA+H+++RATGG +++TP P AQSALRALARSGMKIGRIEDVTPIP D+T
Sbjct:    1 MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQKCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDST 140          
BLAST of 40S ribosomal protein S14 vs. Ensembl Zebrafish
Match: mrps11 (mitochondrial ribosomal protein S11 [Source:ZFIN;Acc:ZDB-GENE-040724-84])

HSP 1 Score: 48.1358 bits (113), Expect = 1.095e-6
Identity = 34/113 (30.09%), Postives = 58/113 (51.33%), Query Frame = 2
Query:  152 EVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHD 490
            E   + H+ A++N+T + VTD  G +  VR + G +   +  +S+P AA  A    A + +  G+T V V V+  G  +         SA++ L   G+++  I D TP+PH+
Sbjct:   86 EELPIAHVKATYNNTHIQVTDCAG-QYMVRTSCGTEGFKNVKKSTPIAAQTAGISAAAKARAKGVTYVRVLVKGLGPGR--------FSAIKGLTMGGLEVVSITDNTPVPHN 189          
BLAST of 40S ribosomal protein S14 vs. Ensembl Xenopus
Match: rps14 (ribosomal protein S14 [Source:NCBI gene;Acc:549476])

HSP 1 Score: 204.912 bits (520), Expect = 1.160e-67
Identity = 108/140 (77.14%), Postives = 125/140 (89.29%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA ++   KK E+ +SLGP+V EGE VFGVCHIFASFNDTFVHVTD++G ET  RVTGGMKVKADRDESSPYAAMLAAQDVA+RCK+LGITA+H+++RATGG +++TP P AQSALRALARSGMKIGRIEDVTPIP D+T
Sbjct:    1 MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDST 140          
BLAST of 40S ribosomal protein S14 vs. Ensembl Mouse
Match: Rps14 (ribosomal protein S14 [Source:MGI Symbol;Acc:MGI:98107])

HSP 1 Score: 204.912 bits (520), Expect = 8.786e-68
Identity = 108/140 (77.14%), Postives = 125/140 (89.29%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA ++   KK E+ +SLGP+V EGE VFGVCHIFASFNDTFVHVTD++G ET  RVTGGMKVKADRDESSPYAAMLAAQDVA+RCK+LGITA+H+++RATGG +++TP P AQSALRALARSGMKIGRIEDVTPIP D+T
Sbjct:    1 MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDST 140          
BLAST of 40S ribosomal protein S14 vs. Ensembl Mouse
Match: Rps14 (ribosomal protein S14 [Source:MGI Symbol;Acc:MGI:98107])

HSP 1 Score: 204.912 bits (520), Expect = 8.786e-68
Identity = 108/140 (77.14%), Postives = 125/140 (89.29%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA ++   KK E+ +SLGP+V EGE VFGVCHIFASFNDTFVHVTD++G ET  RVTGGMKVKADRDESSPYAAMLAAQDVA+RCK+LGITA+H+++RATGG +++TP P AQSALRALARSGMKIGRIEDVTPIP D+T
Sbjct:    1 MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDST 140          
BLAST of 40S ribosomal protein S14 vs. Ensembl Mouse
Match: Rps14 (ribosomal protein S14 [Source:MGI Symbol;Acc:MGI:98107])

HSP 1 Score: 204.912 bits (520), Expect = 8.786e-68
Identity = 108/140 (77.14%), Postives = 125/140 (89.29%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA ++   KK E+ +SLGP+V EGE VFGVCHIFASFNDTFVHVTD++G ET  RVTGGMKVKADRDESSPYAAMLAAQDVA+RCK+LGITA+H+++RATGG +++TP P AQSALRALARSGMKIGRIEDVTPIP D+T
Sbjct:    1 MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDST 140          
BLAST of 40S ribosomal protein S14 vs. Ensembl Mouse
Match: Rps14 (ribosomal protein S14 [Source:MGI Symbol;Acc:MGI:98107])

HSP 1 Score: 204.912 bits (520), Expect = 8.786e-68
Identity = 108/140 (77.14%), Postives = 125/140 (89.29%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA ++   KK E+ +SLGP+V EGE VFGVCHIFASFNDTFVHVTD++G ET  RVTGGMKVKADRDESSPYAAMLAAQDVA+RCK+LGITA+H+++RATGG +++TP P AQSALRALARSGMKIGRIEDVTPIP D+T
Sbjct:    1 MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDST 140          
BLAST of 40S ribosomal protein S14 vs. Ensembl Mouse
Match: Rps14 (ribosomal protein S14 [Source:MGI Symbol;Acc:MGI:98107])

HSP 1 Score: 204.912 bits (520), Expect = 8.786e-68
Identity = 108/140 (77.14%), Postives = 125/140 (89.29%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA ++   KK E+ +SLGP+V EGE VFGVCHIFASFNDTFVHVTD++G ET  RVTGGMKVKADRDESSPYAAMLAAQDVA+RCK+LGITA+H+++RATGG +++TP P AQSALRALARSGMKIGRIEDVTPIP D+T
Sbjct:    1 MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDST 140          
BLAST of 40S ribosomal protein S14 vs. UniProt/SwissProt
Match: sp|P62264|RS14_MOUSE (40S ribosomal protein S14 OS=Mus musculus OX=10090 GN=Rps14 PE=1 SV=3)

HSP 1 Score: 204.912 bits (520), Expect = 6.146e-67
Identity = 108/140 (77.14%), Postives = 125/140 (89.29%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA ++   KK E+ +SLGP+V EGE VFGVCHIFASFNDTFVHVTD++G ET  RVTGGMKVKADRDESSPYAAMLAAQDVA+RCK+LGITA+H+++RATGG +++TP P AQSALRALARSGMKIGRIEDVTPIP D+T
Sbjct:    1 MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDST 140          
BLAST of 40S ribosomal protein S14 vs. UniProt/SwissProt
Match: sp|P62263|RS14_HUMAN (40S ribosomal protein S14 OS=Homo sapiens OX=9606 GN=RPS14 PE=1 SV=3)

HSP 1 Score: 204.912 bits (520), Expect = 6.146e-67
Identity = 108/140 (77.14%), Postives = 125/140 (89.29%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA ++   KK E+ +SLGP+V EGE VFGVCHIFASFNDTFVHVTD++G ET  RVTGGMKVKADRDESSPYAAMLAAQDVA+RCK+LGITA+H+++RATGG +++TP P AQSALRALARSGMKIGRIEDVTPIP D+T
Sbjct:    1 MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDST 140          
BLAST of 40S ribosomal protein S14 vs. UniProt/SwissProt
Match: sp|P62265|RS14_CRIGR (40S ribosomal protein S14 OS=Cricetulus griseus OX=10029 GN=RPS14 PE=2 SV=3)

HSP 1 Score: 204.912 bits (520), Expect = 6.146e-67
Identity = 108/140 (77.14%), Postives = 125/140 (89.29%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA ++   KK E+ +SLGP+V EGE VFGVCHIFASFNDTFVHVTD++G ET  RVTGGMKVKADRDESSPYAAMLAAQDVA+RCK+LGITA+H+++RATGG +++TP P AQSALRALARSGMKIGRIEDVTPIP D+T
Sbjct:    1 MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDST 140          
BLAST of 40S ribosomal protein S14 vs. UniProt/SwissProt
Match: sp|P13471|RS14_RAT (40S ribosomal protein S14 OS=Rattus norvegicus OX=10116 GN=Rps14 PE=2 SV=3)

HSP 1 Score: 204.527 bits (519), Expect = 7.734e-67
Identity = 108/140 (77.14%), Postives = 125/140 (89.29%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA ++   KK E+ +SLGP+V EGE VFGVCHIFASFNDTFVHVTD++G ET  RVTGGMKVKADRDESSPYAAMLAAQDVA+RCK+LGITA+H+++RATGG +++TP P AQSALRALARSGMKIGRIEDVTPIP D+T
Sbjct:    1 MAPRKGKDKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDST 140          
BLAST of 40S ribosomal protein S14 vs. UniProt/SwissProt
Match: sp|P42036|RS143_ARATH (40S ribosomal protein S14-3 OS=Arabidopsis thaliana OX=3702 GN=RPS14C PE=2 SV=2)

HSP 1 Score: 199.904 bits (507), Expect = 5.572e-65
Identity = 107/138 (77.54%), Postives = 123/138 (89.13%), Query Frame = 2
Query:   83 SKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            SKRK  +   E V+LGP V EGE+VFGV H+FASFNDTF+HVTD++G ET VR+TGGMKVKADRDESSPYAAMLAAQDVA+RCK+LGITA+HV++RATGG K++TP P AQSALRALARSGMKIGRIEDVTPIP D+T
Sbjct:    2 SKRKTKEPKVENVTLGPAVREGEQVFGVVHVFASFNDTFIHVTDLSGRETLVRITGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITAIHVKLRATGGNKTKTPGPGAQSALRALARSGMKIGRIEDVTPIPTDST 139          
BLAST of 40S ribosomal protein S14 vs. TrEMBL
Match: A0A5J4N870 (Small subunit ribosomal protein S14e (Fragment) OS=Paragonimus westermani OX=34504 GN=DEA37_0008179 PE=3 SV=1)

HSP 1 Score: 218.779 bits (556), Expect = 6.241e-70
Identity = 121/141 (85.82%), Postives = 131/141 (92.91%), Query Frame = 2
Query:   77 MASKRKNVKKV-EEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA+KRK  KKV EEQVSLGP+V EGE VFGVCHI+ASFNDTFVHVTD TGAET VRVTGGMKVKADRDESSPYAAMLAAQDVAE+CK LGI A+H+R+RATGGTK+RTPSPAAQSALRALARSGM+IGRIEDVTPIP D+T
Sbjct:    6 MAAKRKTAKKVVEEQVSLGPQVAEGEFVFGVCHIYASFNDTFVHVTDPTGAETIVRVTGGMKVKADRDESSPYAAMLAAQDVAEKCKLLGINALHIRIRATGGTKARTPSPAAQSALRALARSGMRIGRIEDVTPIPTDST 146          
BLAST of 40S ribosomal protein S14 vs. TrEMBL
Match: C1LW84 (40S ribosomal protein OS=Schistosoma japonicum OX=6182 GN=rps14 PE=2 SV=1)

HSP 1 Score: 218.009 bits (554), Expect = 1.270e-69
Identity = 121/141 (85.82%), Postives = 130/141 (92.20%), Query Frame = 2
Query:   77 MASKRKNVKKV-EEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA+KRK  KKV EEQVSLGP+V EGE VFGVCHI+ASFNDTFVHVTD TGAET VRVTGGMKVKADRDESSPYAAMLAAQDVAE+CK LGI A+H+R+RATGGTK RTPSPAAQSALRALARSGM+IGRIEDVTPIP D+T
Sbjct:    1 MAAKRKGAKKVVEEQVSLGPQVAEGEFVFGVCHIYASFNDTFVHVTDATGAETIVRVTGGMKVKADRDESSPYAAMLAAQDVAEKCKALGINALHIRIRATGGTKVRTPSPAAQSALRALARSGMRIGRIEDVTPIPTDST 141          
BLAST of 40S ribosomal protein S14 vs. TrEMBL
Match: A0A183BFM2 (Uncharacterized protein OS=Echinostoma caproni OX=27848 GN=ECPE_LOCUS18007 PE=3 SV=1)

HSP 1 Score: 217.624 bits (553), Expect = 1.448e-69
Identity = 118/141 (83.69%), Postives = 131/141 (92.91%), Query Frame = 2
Query:   77 MASKRKNVKKV-EEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA+KRK  KKV EEQ+SLGP+V EGE VFG+CHI+ASFNDTFVHVTD TGAET VRVTGGMKVKADRDESSPYAAMLAAQDVAE+C+ LGI A+H+R+RATGGTK+RTPSPAAQSALRALARSGM+IGRIEDVTPIP D+T
Sbjct:    1 MAAKRKTAKKVVEEQISLGPQVAEGEXVFGICHIYASFNDTFVHVTDPTGAETIVRVTGGMKVKADRDESSPYAAMLAAQDVAEKCRLLGINALHIRIRATGGTKARTPSPAAQSALRALARSGMRIGRIEDVTPIPTDST 141          
BLAST of 40S ribosomal protein S14 vs. TrEMBL
Match: A0A183VVW2 (Uncharacterized protein OS=Trichobilharzia regenti OX=157069 GN=TRE_LOCUS4618 PE=3 SV=1)

HSP 1 Score: 217.238 bits (552), Expect = 1.989e-69
Identity = 120/141 (85.11%), Postives = 130/141 (92.20%), Query Frame = 2
Query:   77 MASKRKNVKKV-EEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA+KRK  KKV EEQ+SLGP+V EGE VFGVCHI+ASFNDTFVHVTD TGAET VRVTGGMKVKADRDESSPYAAMLAAQDVAE+CK LGI A+H+R+RATGGTK RTPSPAAQSALRALARSGM+IGRIEDVTPIP D+T
Sbjct:    1 MAAKRKGAKKVVEEQISLGPQVAEGEFVFGVCHIYASFNDTFVHVTDATGAETIVRVTGGMKVKADRDESSPYAAMLAAQDVAEKCKTLGINALHIRIRATGGTKVRTPSPAAQSALRALARSGMRIGRIEDVTPIPTDST 141          
BLAST of 40S ribosomal protein S14 vs. TrEMBL
Match: A0A094ZQN1 (40S ribosomal protein S14 OS=Schistosoma haematobium OX=6185 GN=MS3_03577 PE=3 SV=1)

HSP 1 Score: 216.468 bits (550), Expect = 4.142e-69
Identity = 120/141 (85.11%), Postives = 130/141 (92.20%), Query Frame = 2
Query:   77 MASKRKNVKKV-EEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA+KRK  +KV EEQVSLGP+V EGE VFGVCHI+ASFNDTFVHVTD TGAET VRVTGGMKVKADRDESSPYAAMLAAQDVAE+CK LGI A+H+R+RATGGTK RTPSPAAQSALRALARSGM+IGRIEDVTPIP D+T
Sbjct:    1 MAAKRKGARKVVEEQVSLGPQVAEGEFVFGVCHIYASFNDTFVHVTDATGAETIVRVTGGMKVKADRDESSPYAAMLAAQDVAEKCKTLGINALHIRIRATGGTKVRTPSPAAQSALRALARSGMRIGRIEDVTPIPTDST 141          
BLAST of 40S ribosomal protein S14 vs. Ensembl Cavefish
Match: rps14 (ribosomal protein S14 [Source:NCBI gene;Acc:103026869])

HSP 1 Score: 204.912 bits (520), Expect = 6.900e-68
Identity = 108/140 (77.14%), Postives = 125/140 (89.29%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA ++   KK E+ +SLGP+V EGE VFGVCHIFASFNDTFVHVTD++G ET  RVTGGMKVKADRDESSPYAAMLAAQDVA+RCK+LGITA+H+++RATGG +++TP P AQSALRALARSGMKIGRIEDVTPIP D+T
Sbjct:    1 MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDST 140          
BLAST of 40S ribosomal protein S14 vs. Ensembl Cavefish
Match: rps14 (ribosomal protein S14 [Source:NCBI gene;Acc:103026869])

HSP 1 Score: 172.555 bits (436), Expect = 1.090e-53
Identity = 78/103 (75.73%), Postives = 91/103 (88.35%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGT 385
            MA ++   KK E+ +SLGP+V EGE VFGVCHIFASFNDTFVHVTD++G ET  RVTGGMKVKADRDESSPYAAMLAAQDVA+RCK+LGITA+H+++RATGG 
Sbjct:    1 MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGN 103          
BLAST of 40S ribosomal protein S14 vs. Ensembl Cavefish
Match: mrps11 (mitochondrial ribosomal protein S11 [Source:NCBI gene;Acc:103047566])

HSP 1 Score: 48.1358 bits (113), Expect = 9.501e-7
Identity = 34/113 (30.09%), Postives = 59/113 (52.21%), Query Frame = 2
Query:  152 EVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHD 490
            E   + H+ A++N+T + VTD  G ++ VR + G +   +  +S+P AA  A    A + +  G+T   V V+  G  +         SA++ L+  G++I  I D TP+PH+
Sbjct:   91 EDLHIIHVKATYNNTHIQVTDNLG-QSMVRTSCGTEGFRNSKKSTPIAAQTAGISAASKAQAKGVTFARVLVKGLGPGR--------LSAIKGLSMGGLQIVSITDNTPVPHN 194          
BLAST of 40S ribosomal protein S14 vs. Ensembl Yeast
Match: RPS14A (Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication [Source:SGD;Acc:S000000627])

HSP 1 Score: 169.088 bits (427), Expect = 6.445e-55
Identity = 88/118 (74.58%), Postives = 106/118 (89.83%), Query Frame = 2
Query:  143 EGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            +  +VFGV  I+ASFNDTFVHVTD++G ET  RVTGGMKVKADRDESSPYAAMLAAQDVA +CK++GITAVHV++RATGGT+++TP P  Q+ALRALARSG++IGRIEDVTP+P D+T
Sbjct:    9 DNSQVFGVARIYASFNDTFVHVTDLSGKETIARVTGGMKVKADRDESSPYAAMLAAQDVAAKCKEVGITAVHVKIRATGGTRTKTPGPGGQAALRALARSGLRIGRIEDVTPVPSDST 126          
BLAST of 40S ribosomal protein S14 vs. Ensembl Yeast
Match: RPS14B (Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication [Source:SGD;Acc:S000003727])

HSP 1 Score: 169.088 bits (427), Expect = 7.026e-55
Identity = 88/118 (74.58%), Postives = 106/118 (89.83%), Query Frame = 2
Query:  143 EGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            +  +VFGV  I+ASFNDTFVHVTD++G ET  RVTGGMKVKADRDESSPYAAMLAAQDVA +CK++GITAVHV++RATGGT+++TP P  Q+ALRALARSG++IGRIEDVTP+P D+T
Sbjct:   10 DNSQVFGVARIYASFNDTFVHVTDLSGKETIARVTGGMKVKADRDESSPYAAMLAAQDVAAKCKEVGITAVHVKIRATGGTRTKTPGPGGQAALRALARSGLRIGRIEDVTPVPSDST 127          
BLAST of 40S ribosomal protein S14 vs. Ensembl Nematostella
Match: EDO42012 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S2J5])

HSP 1 Score: 189.119 bits (479), Expect = 3.521e-62
Identity = 106/141 (75.18%), Postives = 123/141 (87.23%), Query Frame = 2
Query:   83 SKRKNVKKV--EEQVSLGPKVT-EGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            + RK  K+V  E+ +SLGP+V  EGE VFGV HIFASFNDTFVHVTD++G ET  RVTGGMKVKADRDE+SPYAAMLAAQDVA RCK++GITA+H+++RATGG K++TP P AQSALRALARSGMKIGRIEDVTPIP D+T
Sbjct:    2 APRKGKKEVKEEQNISLGPQVVGEGELVFGVAHIFASFNDTFVHVTDLSGRETISRVTGGMKVKADRDEASPYAAMLAAQDVAARCKEIGITALHIKLRATGGNKTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDST 142          
BLAST of 40S ribosomal protein S14 vs. Ensembl Nematostella
Match: EDO25642 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8DVW8])

HSP 1 Score: 55.8398 bits (133), Expect = 1.646e-10
Identity = 32/110 (29.09%), Postives = 54/110 (49.09%), Query Frame = 2
Query:  161 GVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHD 490
            G+ H+ ASFN+T + +TD  G       +GG   K  R +S+P+AA +A++       + GI  + V ++  G  +  +               G++I  I DVTP+PH+
Sbjct:   15 GIAHVHASFNNTIITITDRQGNALSWASSGGQGFKGSR-KSTPFAAQVASEVAGRAAIEQGIKNLDVEIKGPGPGRESSVRALGAL--------GIRITSIADVTPVPHN 115          
BLAST of 40S ribosomal protein S14 vs. Ensembl Medaka
Match: rps14 (ribosomal protein S14 [Source:NCBI gene;Acc:101157558])

HSP 1 Score: 204.912 bits (520), Expect = 6.857e-68
Identity = 108/140 (77.14%), Postives = 125/140 (89.29%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MA ++   KK E+ +SLGP+V EGE VFGVCHIFASFNDTFVHVTD++G ET  RVTGGMKVKADRDESSPYAAMLAAQDVA+RCK+LGITA+H+++RATGG +++TP P AQSALRALARSGMKIGRIEDVTPIP D+T
Sbjct:    1 MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDST 140          
BLAST of 40S ribosomal protein S14 vs. Ensembl Medaka
Match: mrps11 (mitochondrial ribosomal protein S11 [Source:NCBI gene;Acc:101169186])

HSP 1 Score: 46.595 bits (109), Expect = 2.763e-6
Identity = 35/113 (30.97%), Postives = 59/113 (52.21%), Query Frame = 2
Query:  152 EVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHD 490
            E   + HI A++N+T + +TD  G ++ VR + G +   +  +S+P AA  A    A +    G+T V V V+  G  +         SA++ LA  G+++  I D TP+PH+
Sbjct:   82 EELPIVHIKATYNNTHIQLTDSEG-QSLVRTSCGTEGFKNIKKSTPIAAQTAGISAAAKATGKGVTFVRVVVKGIGPGRQ--------SAIKGLAMGGLEVVSITDNTPVPHN 185          
BLAST of 40S ribosomal protein S14 vs. Ensembl Medaka
Match: mrps11 (mitochondrial ribosomal protein S11 [Source:NCBI gene;Acc:101169186])

HSP 1 Score: 45.8246 bits (107), Expect = 7.177e-6
Identity = 35/113 (30.97%), Postives = 59/113 (52.21%), Query Frame = 2
Query:  152 EVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHD 490
            E   + HI A++N+T + +TD  G ++ VR + G +   +  +S+P AA  A    A +    G+T V V V+  G  +         SA++ LA  G+++  I D TP+PH+
Sbjct:  135 EELPIVHIKATYNNTHIQLTDSEG-QSLVRTSCGTEGFKNIKKSTPIAAQTAGISAAAKATGKGVTFVRVVVKGIGPGRQ--------SAIKGLAMGGLEVVSITDNTPVPHN 238          
BLAST of 40S ribosomal protein S14 vs. Planmine SMEST
Match: SMESG000036550.1 (SMESG000036550.1)

HSP 1 Score: 254.218 bits (648), Expect = 1.362e-87
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTPSPAAQSALRALARSGMKIGRIEDVTPIPHDTT
Sbjct:    1 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTKSRTPSPAAQSALRALARSGMKIGRIEDVTPIPHDTT 140          
BLAST of 40S ribosomal protein S14 vs. Planmine SMEST
Match: SMESG000003565.1 (SMESG000003565.1)

HSP 1 Score: 190.66 bits (483), Expect = 1.807e-62
Identity = 102/141 (72.34%), Postives = 122/141 (86.52%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTK-SRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            MAS+++ VK V E  +LGPK+ +GEE FG+CHI+A  NDTF+HVTDVTGAETFV+VTGGMKVKADRDE SPYAAMLA+QDVA++CK LGITAVHV +R  GG K S  PSPAA +A+RALAR+G+KIGRIE+VTP+PHDTT
Sbjct:    1 MASRKRTVKAVNEVTTLGPKLVDGEENFGLCHIYAGKNDTFIHVTDVTGAETFVKVTGGMKVKADRDEPSPYAAMLASQDVADKCKALGITAVHVEIRGGGGLKRSPAPSPAASTAMRALARAGLKIGRIENVTPVPHDTT 141          
BLAST of 40S ribosomal protein S14 vs. Planmine SMEST
Match: SMESG000003564.1 (SMESG000003564.1)

HSP 1 Score: 189.119 bits (479), Expect = 9.413e-62
Identity = 101/141 (71.63%), Postives = 121/141 (85.82%), Query Frame = 2
Query:   77 MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGAETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRATGGTK-SRTXXXXXXXXXXXXXXSGMKIGRIEDVTPIPHDTT 496
            M S+++ VK V E  +LGPK+ +GEE FG+CHI+A  NDTF+HVTDVTGAETFV+VTGGMKVKADRDE SPYAAMLA+QDVA++CK LGITAVHV +R  GG K S  PSPAA +A+RALAR+G+KIGRIE+VTP+PHDTT
Sbjct:    1 MTSRKRTVKAVNEVTTLGPKLVDGEENFGLCHIYAGKNDTFIHVTDVTGAETFVKVTGGMKVKADRDEPSPYAAMLASQDVADKCKALGITAVHVEIRGGGGLKRSPAPSPAASTAMRALARAGLKIGRIENVTPVPHDTT 141          
The following BLAST results are available for this feature:
BLAST of 40S ribosomal protein S14 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
RPS141.280e-6777.14ribosomal protein S14 [Source:HGNC Symbol;Acc:HGNC... [more]
RPS141.280e-6777.14ribosomal protein S14 [Source:HGNC Symbol;Acc:HGNC... [more]
RPS141.280e-6777.14ribosomal protein S14 [Source:HGNC Symbol;Acc:HGNC... [more]
RPS141.280e-6777.14ribosomal protein S14 [Source:HGNC Symbol;Acc:HGNC... [more]
RPS141.367e-6777.14ribosomal protein S14 [Source:HGNC Symbol;Acc:HGNC... [more]
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BLAST of 40S ribosomal protein S14 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rps-141.343e-6475.8940S ribosomal protein S14 [Source:UniProtKB/Swiss... [more]
rps-141.343e-6475.8940S ribosomal protein S14 [Source:UniProtKB/Swiss... [more]
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BLAST of 40S ribosomal protein S14 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 4
Match NameE-valueIdentityDescription
RpS14a3.321e-6475.00gene:FBgn0004403 transcript:FBtr0071095[more]
RpS14a3.321e-6475.00gene:FBgn0004403 transcript:FBtr0071094[more]
RpS14b3.321e-6475.00gene:FBgn0004404 transcript:FBtr0071096[more]
RpS14b3.321e-6475.00gene:FBgn0004404 transcript:FBtr0345291[more]
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BLAST of 40S ribosomal protein S14 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
rps142.772e-6776.43ribosomal protein S14 [Source:ZFIN;Acc:ZDB-GENE-03... [more]
mrps111.095e-630.09mitochondrial ribosomal protein S11 [Source:ZFIN;A... [more]
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BLAST of 40S ribosomal protein S14 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rps141.160e-6777.14ribosomal protein S14 [Source:NCBI gene;Acc:549476... [more]
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BLAST of 40S ribosomal protein S14 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Rps148.786e-6877.14ribosomal protein S14 [Source:MGI Symbol;Acc:MGI:9... [more]
Rps148.786e-6877.14ribosomal protein S14 [Source:MGI Symbol;Acc:MGI:9... [more]
Rps148.786e-6877.14ribosomal protein S14 [Source:MGI Symbol;Acc:MGI:9... [more]
Rps148.786e-6877.14ribosomal protein S14 [Source:MGI Symbol;Acc:MGI:9... [more]
Rps148.786e-6877.14ribosomal protein S14 [Source:MGI Symbol;Acc:MGI:9... [more]
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BLAST of 40S ribosomal protein S14 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P62264|RS14_MOUSE6.146e-6777.1440S ribosomal protein S14 OS=Mus musculus OX=10090... [more]
sp|P62263|RS14_HUMAN6.146e-6777.1440S ribosomal protein S14 OS=Homo sapiens OX=9606 ... [more]
sp|P62265|RS14_CRIGR6.146e-6777.1440S ribosomal protein S14 OS=Cricetulus griseus OX... [more]
sp|P13471|RS14_RAT7.734e-6777.1440S ribosomal protein S14 OS=Rattus norvegicus OX=... [more]
sp|P42036|RS143_ARATH5.572e-6577.5440S ribosomal protein S14-3 OS=Arabidopsis thalian... [more]
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BLAST of 40S ribosomal protein S14 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5J4N8706.241e-7085.82Small subunit ribosomal protein S14e (Fragment) OS... [more]
C1LW841.270e-6985.8240S ribosomal protein OS=Schistosoma japonicum OX=... [more]
A0A183BFM21.448e-6983.69Uncharacterized protein OS=Echinostoma caproni OX=... [more]
A0A183VVW21.989e-6985.11Uncharacterized protein OS=Trichobilharzia regenti... [more]
A0A094ZQN14.142e-6985.1140S ribosomal protein S14 OS=Schistosoma haematobi... [more]
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BLAST of 40S ribosomal protein S14 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
rps146.900e-6877.14ribosomal protein S14 [Source:NCBI gene;Acc:103026... [more]
rps141.090e-5375.73ribosomal protein S14 [Source:NCBI gene;Acc:103026... [more]
mrps119.501e-730.09mitochondrial ribosomal protein S11 [Source:NCBI g... [more]
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BLAST of 40S ribosomal protein S14 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 40S ribosomal protein S14 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
RPS14A6.445e-5574.58Protein component of the small (40S) ribosomal sub... [more]
RPS14B7.026e-5574.58Protein component of the small (40S) ribosomal sub... [more]
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BLAST of 40S ribosomal protein S14 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO420123.521e-6275.18Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO256421.646e-1029.09Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8... [more]
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BLAST of 40S ribosomal protein S14 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
rps146.857e-6877.14ribosomal protein S14 [Source:NCBI gene;Acc:101157... [more]
mrps112.763e-630.97mitochondrial ribosomal protein S11 [Source:NCBI g... [more]
mrps117.177e-630.97mitochondrial ribosomal protein S11 [Source:NCBI g... [more]
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BLAST of 40S ribosomal protein S14 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000036550.11.362e-87100.00SMESG000036550.1[more]
SMESG000003565.11.807e-6272.34SMESG000003565.1[more]
SMESG000003564.19.413e-6271.63SMESG000003564.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024202 ID=SMED30024202|Name=40S ribosomal protein S14|organism=Schmidtea mediterranea sexual|type=transcript|length=569bp
ATTTATATTTCCATAAACGATTACATAAGCAATTGTTTAAATTATTATTT
GCTGTTAACTAAATCAAATTTAAATAATGGCTTCTAAAAGAAAGAATGTC
AAAAAAGTCGAAGAGCAAGTTTCGCTTGGACCAAAGGTAACTGAAGGAGA
AGAAGTTTTTGGTGTTTGTCATATTTTTGCGTCTTTCAATGATACTTTTG
TTCATGTTACTGATGTTACTGGAGCAGAAACTTTTGTCCGGGTTACAGGA
GGTATGAAAGTCAAAGCAGACAGAGATGAAAGTTCTCCTTATGCTGCTAT
GCTTGCAGCTCAAGACGTTGCTGAGCGATGCAAACAGTTAGGAATAACTG
CTGTTCACGTTAGAGTTCGTGCTACTGGTGGTACTAAATCACGAACACCA
AGTCCCGCTGCACAATCTGCACTTCGTGCTCTTGCAAGAAGCGGTATGAA
AATTGGAAGAATAGAAGATGTGACTCCTATTCCTCATGATACAACCAGAA
GAAAAGGAGGTAGAAGAGGTCGTAGATTGTAGTTTTAAAATAAATATTTG
GATGTAAAAAAAAAGATCG
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protein sequence of SMED30024202-orf-1

>SMED30024202-orf-1 ID=SMED30024202-orf-1|Name=SMED30024202-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=152bp
MASKRKNVKKVEEQVSLGPKVTEGEEVFGVCHIFASFNDTFVHVTDVTGA
ETFVRVTGGMKVKADRDESSPYAAMLAAQDVAERCKQLGITAVHVRVRAT
GGTKSRTPSPAAQSALRALARSGMKIGRIEDVTPIPHDTTRRKGGRRGRR
L*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000120progenitor cell
PLANA:0000429neoblast
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR036967Ribosomal_S11_sf
IPR001971Ribosomal_S11
IPR018102Ribosomal_S11_CS
Vocabulary: molecular function
TermDefinition
GO:0003735structural constituent of ribosome
Vocabulary: biological process
TermDefinition
GO:0006412translation
Vocabulary: cellular component
TermDefinition
GO:0005840ribosome
GO:0030529intracellular ribonucleoprotein complex
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036967Ribosomal protein S11 superfamilyGENE3DG3DSA:3.30.420.80coord: 1..151
e-value: 1.7E-79
score: 266.7
IPR001971Ribosomal protein S11PIRSFPIRSF002131RPS11p_RPS11a_RPS14e_RPS11ocoord: 1..148
e-value: 7.1E-38
score: 127.2
IPR001971Ribosomal protein S11PFAMPF00411Ribosomal_S11coord: 29..147
e-value: 4.3E-46
score: 155.8
IPR001971Ribosomal protein S11PANTHERPTHR1175940S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11coord: 3..151
NoneNo IPR availablePANTHERPTHR11759:SF28coord: 3..151
NoneNo IPR availableSUPERFAMILYSSF53137Translational machinery componentscoord: 24..149
IPR018102Ribosomal S11, conserved sitePROSITEPS00054RIBOSOMAL_S11coord: 116..138