SMED30024169

Overview
NameSMED30024169
Smed IDSMED30024169
Length (bp)566
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30024169 (SMED30024169) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30024169 (SMED30024169) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30024169

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30024169SMESG000016970.1 dd_Smed_v4_2205_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30024169SMESG000016970.1 dd_Smed_v4_2205_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30024169SMESG000016970.1 dd_Smed_v4_2205_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30024169SMESG000016970.1 dd_Smed_v4_2205_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30024169SMESG000016970.1 dd_Smed_v4_2205_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30024169SMESG000016970.1 dd_Smed_v4_2205_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30024169SMESG000016970.1 Contig45893newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30024169SMESG000016970.1 Contig45893uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
epidermisSMED30024169SMESG000016970.1 dd_Smed_v4_2205_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
dorsal region of the epidermisSMED30024169SMESG000016970.1 dd_Smed_v4_2205_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30024169 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001810:60330..65306 -1923
Homology
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BLAST of SMED30024169 vs. Planmine SMEST
Match: SMESG000016970.1 (SMESG000016970.1)

HSP 1 Score: 217.624 bits (553), Expect = 5.481e-68
Identity = 130/169 (76.92%), Postives = 130/169 (76.92%), Query Frame = 2 
Query:   59 MRSFQMDKSSDSGVSDRKPGLDPFQPANESFEISNKYDPSSQMDIISDSGVFDRTPGLSFKLANVMSVGXXXXXXXXXXXXXGVSDRKPGLDPFQPANESFEISNKYDPSSQMDIISDSGVFDRTPGLDPAVKESKEKIDNNFYSNRMSQDVDSRPSYSGDIQGIHSKF 565
            MRSFQMDKSSDSGVSDRKPGLDPFQPANESFEISNKYDPSSQMDIISDSGVFDRTPGLSFKLANVMSVGKSKKYDMDKSSDSGV DR PGL                                     DPAVKESKEKIDNNFYSNRMSQDVDSRPSYSGDIQGIHSKF
Sbjct:    1 MRSFQMDKSSDSGVSDRKPGLDPFQPANESFEISNKYDPSSQMDIISDSGVFDRTPGLSFKLANVMSVGKSKKYDMDKSSDSGVFDRTPGL-------------------------------------DPAVKESKEKIDNNFYSNRMSQDVDSRPSYSGDIQGIHSKF 132
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BLAST of SMED30024169 vs. Planmine SMEST
Match: SMESG000016970.1 (SMESG000016970.1)

HSP 1 Score: 207.223 bits (526), Expect = 6.198e-64
Identity = 125/164 (76.22%), Postives = 125/164 (76.22%), Query Frame = 2 
Query:   74 MDKSSDSGVSDRKPGLDPFQPANESFEISNKYDPSSQMDIISDSGVFDRTPGLSFKLANVMSVGXXXXXXXXXXXXXGVSDRKPGLDPFQPANESFEISNKYDPSSQMDIISDSGVFDRTPGLDPAVKESKEKIDNNFYSNRMSQDVDSRPSYSGDIQGIHSKF 565
            MDKSSDSGVSDRKPGLDPFQPANESFEISNKYDPSSQMDIISDSGVFDRTPGLSFKLANVMSVGKSKKYDMDKSSDSGV DR PGL                                     DPAVKESKEKIDNNFYSNRMSQDVDSRPSYSGDIQGIHSKF
Sbjct:    1 MDKSSDSGVSDRKPGLDPFQPANESFEISNKYDPSSQMDIISDSGVFDRTPGLSFKLANVMSVGKSKKYDMDKSSDSGVFDRTPGL-------------------------------------DPAVKESKEKIDNNFYSNRMSQDVDSRPSYSGDIQGIHSKF 127

HSP 2 Score: 51.2174 bits (121), Expect = 9.970e-8
Identity = 35/55 (63.64%), Postives = 36/55 (65.45%), Query Frame = 2 
Query:   71 QMDKSSDSGVSDRKPGLDPFQPAN-ESFEISNKYDPSSQMDIISDSGVFDRTPGL 232
            QMD  SDSGV DR PGL  F+ AN  S   S KYD    MD  SDSGVFDRTPGL
Sbjct:   37 QMDIISDSGVFDRTPGLS-FKLANVMSVGKSKKYD----MDKSSDSGVFDRTPGL 86
The following BLAST results are available for this feature:
BLAST of SMED30024169 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024169 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024169 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024169 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024169 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024169 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024169 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024169 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024169 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024169 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024169 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024169 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024169 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024169 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000016970.15.481e-6876.92SMESG000016970.1[more]
SMESG000016970.16.198e-6476.22SMESG000016970.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024169 ID=SMED30024169|Name=SMED30024169|organism=Schmidtea mediterranea sexual|type=transcript|length=566bp
CCTGGCCTGATACCCTTTCTTACTTATTTTATGCAAATTAAATTACATTT
ATGTTGAAATGCGCTCATTTCAGATGGATAAAAGCAGTGATTCTGGAGTA
TCTGACAGGAAGCCGGGATTGGATCCATTTCAACCTGCAAACGAATCGTT
TGAAATCTCAAATAAATATGATCCCTCATCCCAGATGGATATAATCAGTG
ATTCTGGAGTATTTGACAGGACGCCGGGATTATCATTTAAACTTGCAAAC
GTAATGTCGGTTGGAAAGTCTAAGAAATATGATATGGATAAAAGCAGTGA
TTCTGGAGTATCTGACAGGAAGCCGGGATTGGATCCATTTCAACCTGCAA
ACGAATCGTTTGAAATCTCAAATAAATATGATCCCTCATCCCAGATGGAT
ATAATCAGTGATTCTGGAGTATTTGACAGGACGCCGGGATTGGATCCAGC
TGTAAAAGAAAGTAAAGAAAAAATTGATAACAATTTCTACTCAAATAGAA
TGTCACAGGACGTTGATAGTCGCCCGAGCTATAGTGGAGATATTCAAGGC
ATACATTCAAAGTTTG
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protein sequence of SMED30024169-orf-1

>SMED30024169-orf-1 ID=SMED30024169-orf-1|Name=SMED30024169-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=169bp
MRSFQMDKSSDSGVSDRKPGLDPFQPANESFEISNKYDPSSQMDIISDSG
VFDRTPGLSFKLANVMSVGKSKKYDMDKSSDSGVSDRKPGLDPFQPANES
FEISNKYDPSSQMDIISDSGVFDRTPGLDPAVKESKEKIDNNFYSNRMSQ
DVDSRPSYSGDIQGIHSKF
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000054dorsal region of the epidermis
PLANA:0000099neuron
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell