SMED30024157

Overview


Name	SMED30024157
Smed ID	SMED30024157
Length (bp)	1667

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of SMED30024157 (SMED30024157) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30024157 (SMED30024157) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30024157 (SMED30024157) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30024157 (SMED30024157) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30024157

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 5

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
parapharyngeal region	SMED30024157	h1SMcG0009588	SmedASXL_015504	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
epidermis	SMED30024157	h1SMcG0009588	dd_Smed_v4_12598_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30024157	h1SMcG0009588	dd_Smed_v4_12598_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30024157	h1SMcG0009588	dd_Smed_v4_12598_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
enteric muscle cell	SMED30024157	h1SMcG0009588	dd_Smed_v4_12598_0_1	dd_Smed_v4	PMID:30471994 Scimone et al., 2018

Note: Hover over icons to view figure legend

Alignments

SMED30024157 aligns in the following genomic locations:


Alignment Location	Alignment Score
v31.000487:147532..158203 -	7075

Homology

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BLAST of SMED30024157 vs. Ensembl Human
Match: LINGO2 (leucine rich repeat and Ig domain containing 2 [Source:HGNC Symbol;Acc:HGNC:21207])

HSP 1 Score: 56.225 bits (134), Expect = 1.119e-7
Identity = 91/426 (21.36%), Postives = 175/426 (41.08%), Query Frame = 2

Query:  248 FQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNIST--TSIXXXXXXXXXXXXXXXWNNNLKRLKFSSDY-FKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIG--FHKMFPYIFQNLSYL---DASSNRIVKLSESMKYVLKFRQII-FSNNPLFCGCELNWLRNSNPKIKIKCSKVIQANDQSDIEVVENYQDPFYPKS-----HCYPPKTYLEIESAKKILKISIAEMTFDLYSCEFIQLRCIAVGDPLPTLEFIN-----FNEATFGKSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYG 1468
            F    NL  + +   R+   P   F  LS L ++ ++E  I  + D +F++  N+K L  +   +L  IS  +      +E++ L   N++   T     L  +          NN+    F   +  KH + D   L+     NS +  NL +  V    N   +   +  +KHL+  T +     N+ +     + +G F+++ +L      I+G     + P+ FQ L +L   + S N +  L E++    +  +++  +NNPL C C L W+    P ++    + + A   +  E   +++D F+  +      C  PK        KK+  + + E        + +QL C A GDP P + ++          + G++++       +      IR +Q  ++  Y    C+ +N+ G
Sbjct:  101 FNNLFNLRSLRLKGNRLKLVPLGVFTGLSNLTKLDISENKIVILLDYMFQDLHNLKSLE-VGDNDLVYISHRAFSGLLSLEQLTLEKCNLTAVPTEALSHLRSLISLHLKHLNINNMPVYAFKRLFHLKHLEIDYWPLLDMMPANSLYGLNLTSLSVT---NTNLSTVPFLAFKHLVYLTHL-----NLSYNPISTIEAGMFSDLIRL--QELHIVGAQLRTIEPHSFQGLRFLRVLNVSQNLLETLEENVFSSPRALEVLSINNNPLACDCRLLWILQRQPTLQFGGQQPMCAGPDTIRE--RSFKD-FHSTALSFYFTCKKPKI-----REKKLQHLLVDE-------GQTVQLECSADGDPQPVISWVTPRRRFITTKSNGRATV-------LGDGTLEIRFAQDQDSGMY---VCIASNAAG 490

[Close]

Query:  248 FQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNIST--TSIXXXXXXXXXXXXXXXWNNNLKRLKFSSDY-FKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIG--FHKMFPYIFQNLSYL---DASSNRIVKLSESMKYVLKFRQII-FSNNPLFCGCELNWLRNSNPKIKIKCSKVIQANDQSDIEVVENYQDPFYPKS-----HCYPPKTYLEIESAKKILKISIAEMTFDLYSCEFIQLRCIAVGDPLPTLEFIN-----FNEATFGKSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYG 1468
            F    NL  + +   R+   P   F  LS L ++ ++E  I  + D +F++  N+K L  +   +L  IS  +      +E++ L   N++   T     L  +          NN+    F   +  KH + D   L+     NS +  NL +  V    N   +   +  +KHL+  T +     N+ +     + +G F+++ +L      I+G     + P+ FQ L +L   + S N +  L E++    +  +++  +NNPL C C L W+    P ++    + + A   +  E   +++D F+  +      C  PK        KK+  + + E        + +QL C A GDP P + ++          + G++++       +      IR +Q  ++  Y    C+ +N+ G
Sbjct:  101 FNNLFNLRSLRLKGNRLKLVPLGVFTGLSNLTKLDISENKIVILLDYMFQDLHNLKSLE-VGDNDLVYISHRAFSGLLSLEQLTLEKCNLTAVPTEALSHLRSLISLHLKHLNINNMPVYAFKRLFHLKHLEIDYWPLLDMMPANSLYGLNLTSLSVT---NTNLSTVPFLAFKHLVYLTHL-----NLSYNPISTIEAGMFSDLIRL--QELHIVGAQLRTIEPHSFQGLRFLRVLNVSQNLLETLEENVFSSPRALEVLSINNNPLACDCRLLWILQRQPTLQFGGQQPMCAGPDTIRE--RSFKD-FHSTALSFYFTCKKPKI-----REKKLQHLLVDE-------GQTVQLECSADGDPQPVISWVTPRRRFITTKSNGRATV-------LGDGTLEIRFAQDQDSGMY---VCIASNAAG 490

[Close]

Query:  248 FQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNIST--TSIXXXXXXXXXXXXXXXWNNNLKRLKFSSDY-FKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIG--FHKMFPYIFQNLSYL---DASSNRIVKLSESMKYVLKFRQII-FSNNPLFCGCELNWLRNSNPKIKIKCSKVIQANDQSDIEVVENYQDPFYPKS-----HCYPPKTYLEIESAKKILKISIAEMTFDLYSCEFIQLRCIAVGDPLPTLEFIN-----FNEATFGKSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYG 1468
            F    NL  + +   R+   P   F  LS L ++ ++E  I  + D +F++  N+K L  +   +L  IS  +      +E++ L   N++   T     L  +          NN+    F   +  KH + D   L+     NS +  NL +  V    N   +   +  +KHL+  T +     N+ +     + +G F+++ +L      I+G     + P+ FQ L +L   + S N +  L E++    +  +++  +NNPL C C L W+    P ++    + + A   +  E   +++D F+  +      C  PK        KK+  + + E        + +QL C A GDP P + ++          + G++++       +      IR +Q  ++  Y    C+ +N+ G
Sbjct:  101 FNNLFNLRSLRLKGNRLKLVPLGVFTGLSNLTKLDISENKIVILLDYMFQDLHNLKSLE-VGDNDLVYISHRAFSGLLSLEQLTLEKCNLTAVPTEALSHLRSLISLHLKHLNINNMPVYAFKRLFHLKHLEIDYWPLLDMMPANSLYGLNLTSLSVT---NTNLSTVPFLAFKHLVYLTHL-----NLSYNPISTIEAGMFSDLIRL--QELHIVGAQLRTIEPHSFQGLRFLRVLNVSQNLLETLEENVFSSPRALEVLSINNNPLACDCRLLWILQRQPTLQFGGQQPMCAGPDTIRE--RSFKD-FHSTALSFYFTCKKPKI-----REKKLQHLLVDE-------GQTVQLECSADGDPQPVISWVTPRRRFITTKSNGRATV-------LGDGTLEIRFAQDQDSGMY---VCIASNAAG 490

[Close]

BLAST of SMED30024157 vs. Ensembl Xenopus
Match: LINGO2 (leucine rich repeat and Ig domain containing 2 [Source:NCBI gene;Acc:100490134])

HSP 1 Score: 51.2174 bits (121), Expect = 4.026e-6
Identity = 88/421 (20.90%), Postives = 172/421 (40.86%), Query Frame = 2

Query:  248 FQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNISTTSIXXXXXXXXXXXXXXXWNNNLKRLKFSSDYFKHFQFDELR---LIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIG--FHKMFPYIFQNLSYL---DASSNRIVKLSESMKYVLKFRQII-FSNNPLFCGCELNWLRNSNPKIKIKCSKVIQAN----DQSDIEVVENYQDPFYPKSHCYPPKTYLEIESAKKILKISIAEMTFDLYSCEFIQLRCIAVGDPLPTLEFINFNEATFGKSSLNFYDEITI-ATSRKTIRHSQLSENSKYNQLHCLGTNSYG 1468
            F    NL  +S+   R+   P   F  LS L ++ ++E  I  + D +F++  ++K L  +    L  IS  +      +E++ L   N++         + +     L + N      F+     H +  E+    L+     NS +  NL    +    N   +   +   KHLI  T +     N+ +    ++ +G F+++ +L      ++G     + P+ FQ L +L   + S N +  L E+     K  +++   +NPL C C L W+   +P +     + + ++     +S  +V++     FY    C  PK        +++L++ + E        + +Q+ C A GDP P + ++     T    SL      T+       IR++Q+ +   Y    C+ +N+ G
Sbjct:  105 FNSLFNLRSLSLKGNRLKLVPLGVFTGLSNLTKLDISENKIVILLDYMFQDLHSLKSLE-VGDNELVYISHRAFSGLVSLEQLTLEKCNLTAVPTDALSHLHNLYSLHLKYLNINVLPPFAFKRLFHLKILEISYWPLLDLVPANSLYGLNLTFLSIT---NTNLSTIPYHALKHLIYLTHL-----NLSYNPIGIIDTGVFSDLVRL--QELHLVGAQLRTIEPHAFQGLRFLRLLNVSQNLLETLEENAFQSPKALEVLCIDDNPLVCDCRLIWMLQRHPMLHFGGQQPMCSSPDSLKESSYKVLDAAVLSFY--FTCKKPKI-----RDREMLQVHVDE-------GQRVQMNCNADGDPQPMISWVTPRRRTV---SLKSNGRTTVLGNGTLEIRYAQIQDTGTY---ICVASNAAG 494

[Close]

BLAST of SMED30024157 vs. Ensembl Mouse
Match: Lingo2 (leucine rich repeat and Ig domain containing 2 [Source:MGI Symbol;Acc:MGI:2442298])

HSP 1 Score: 53.9138 bits (128), Expect = 4.772e-7
Identity = 90/423 (21.28%), Postives = 159/423 (37.59%), Query Frame = 2

Query:  248 FQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNISTTSIXXXXXXXXXXXXXXXWNNNLKRLKFSSDYFKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIG--FHKMFPYIFQNLSYL---DASSNRIVKLSESMKYVLKFRQII-FSNNPLFCGCELNWLRNSNPKIKIKCSKVIQANDQSDIEVVENYQDPFYPKS-----HCYPPKTYLEIESAKKILKISIAEMTFDLYSCEFIQLRCIAVGDPLPTLEFIN-----FNEATFGKSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYG 1468
            F    +L  +++ KC +   P+    +L  L  +HL  LNI  +P   FK           HL NLE I  + L D       +L GLN+++ SI                N NL  + F +  FKH  +                       ++L +N IS                               + +G F+++ +L      I+G     + P+ FQ L +L   + S N +  L E++    +  +++  +NNPL C C L WL    P ++    + + A   +  E   +++D F+  +      C  PK        KK+  + + E        + +QL C A GDP P + ++          + G++++       +      IR +Q  ++  Y    C+ +N+ G
Sbjct:  173 FSGLLSLEQLTLEKCNLTAVPTEALSHLRSLIALHLKHLNINNMPVYAFKR--------LFHLKNLE-IDYWPLLDLMPAN--SLYGLNLTSLSIT---------------NTNLSTVPFLA--FKHLVY--------------------LTHLNLSYNPIST------------------------------IEAGMFSDLIRL--QELHIVGAQLRTIEPHSFQGLRFLRVLNVSQNLLETLEENVFSSPRALEVLSINNNPLACDCRLLWLLQRQPNLQFGGQQPMCAGPDTIRE--RSFKD-FHSTALSFYFTCKKPKI-----REKKLQHLLVDE-------GQTVQLECNADGDPQPVISWVTPRRRFITTKSNGRATV-------LGDGTLEIRFAQDQDSGMY---VCIASNAAG 490

[Close]

Query:  248 FQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNISTTSIXXXXXXXXXXXXXXXWNNNLKRLKFSSDYFKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIG--FHKMFPYIFQNLSYL---DASSNRIVKLSESMKYVLKFRQII-FSNNPLFCGCELNWLRNSNPKIKIKCSKVIQANDQSDIEVVENYQDPFYPKS-----HCYPPKTYLEIESAKKILKISIAEMTFDLYSCEFIQLRCIAVGDPLPTLEFIN-----FNEATFGKSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYG 1468
            F    +L  +++ KC +   P+    +L  L  +HL  LNI  +P   FK           HL NLE I  + L D       +L GLN+++ SI                N NL  + F +  FKH  +                       ++L +N IS                               + +G F+++ +L      I+G     + P+ FQ L +L   + S N +  L E++    +  +++  +NNPL C C L WL    P ++    + + A   +  E   +++D F+  +      C  PK        KK+  + + E        + +QL C A GDP P + ++          + G++++       +      IR +Q  ++  Y    C+ +N+ G
Sbjct:  173 FSGLLSLEQLTLEKCNLTAVPTEALSHLRSLIALHLKHLNINNMPVYAFKR--------LFHLKNLE-IDYWPLLDLMPAN--SLYGLNLTSLSIT---------------NTNLSTVPFLA--FKHLVY--------------------LTHLNLSYNPIST------------------------------IEAGMFSDLIRL--QELHIVGAQLRTIEPHSFQGLRFLRVLNVSQNLLETLEENVFSSPRALEVLSINNNPLACDCRLLWLLQRQPNLQFGGQQPMCAGPDTIRE--RSFKD-FHSTALSFYFTCKKPKI-----REKKLQHLLVDE-------GQTVQLECNADGDPQPVISWVTPRRRFITTKSNGRATV-------LGDGTLEIRFAQDQDSGMY---VCIASNAAG 490

[Close]

Query:  248 FQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNISTTSIXXXXXXXXXXXXXXXWNNNLKRLKFSSDYFKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIG--FHKMFPYIFQNLSYL---DASSNRIVKLSESMKYVLKFRQII-FSNNPLFCGCELNWLRNSNPKIKIKCSKVIQANDQSDIEVVENYQDPFYPKS-----HCYPPKTYLEIESAKKILKISIAEMTFDLYSCEFIQLRCIAVGDPLPTLEFIN-----FNEATFGKSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYG 1468
            F    +L  +++ KC +   P+    +L  L  +HL  LNI  +P   FK           HL NLE I  + L D       +L GLN+++ SI                N NL  + F +  FKH  +                       ++L +N IS                               + +G F+++ +L      I+G     + P+ FQ L +L   + S N +  L E++    +  +++  +NNPL C C L WL    P ++    + + A   +  E   +++D F+  +      C  PK        KK+  + + E        + +QL C A GDP P + ++          + G++++       +      IR +Q  ++  Y    C+ +N+ G
Sbjct:  173 FSGLLSLEQLTLEKCNLTAVPTEALSHLRSLIALHLKHLNINNMPVYAFKR--------LFHLKNLE-IDYWPLLDLMPAN--SLYGLNLTSLSIT---------------NTNLSTVPFLA--FKHLVY--------------------LTHLNLSYNPIST------------------------------IEAGMFSDLIRL--QELHIVGAQLRTIEPHSFQGLRFLRVLNVSQNLLETLEENVFSSPRALEVLSINNNPLACDCRLLWLLQRQPNLQFGGQQPMCAGPDTIRE--RSFKD-FHSTALSFYFTCKKPKI-----REKKLQHLLVDE-------GQTVQLECNADGDPQPVISWVTPRRRFITTKSNGRATV-------LGDGTLEIRFAQDQDSGMY---VCIASNAAG 490

[Close]

Query:  248 FQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNISTTSIXXXXXXXXXXXXXXXWNNNLKRLKFSSDYFKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIG--FHKMFPYIFQNLSYL---DASSNRIVKLSESMKYVLKFRQII-FSNNPLFCGCELNWLRNSNPKIKIKCSKVIQANDQSDIEVVENYQDPFYPKS-----HCYPPKTYLEIESAKKILKISIAEMTFDLYSCEFIQLRCIAVGDPLPTLEFIN-----FNEATFGKSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYG 1468
            F    +L  +++ KC +   P+    +L  L  +HL  LNI  +P   FK           HL NLE I  + L D       +L GLN+++ SI                N NL  + F +  FKH  +                       ++L +N IS                               + +G F+++ +L      I+G     + P+ FQ L +L   + S N +  L E++    +  +++  +NNPL C C L WL    P ++    + + A   +  E   +++D F+  +      C  PK        KK+  + + E        + +QL C A GDP P + ++          + G++++       +      IR +Q  ++  Y    C+ +N+ G
Sbjct:  173 FSGLLSLEQLTLEKCNLTAVPTEALSHLRSLIALHLKHLNINNMPVYAFKR--------LFHLKNLE-IDYWPLLDLMPAN--SLYGLNLTSLSIT---------------NTNLSTVPFLA--FKHLVY--------------------LTHLNLSYNPIST------------------------------IEAGMFSDLIRL--QELHIVGAQLRTIEPHSFQGLRFLRVLNVSQNLLETLEENVFSSPRALEVLSINNNPLACDCRLLWLLQRQPNLQFGGQQPMCAGPDTIRE--RSFKD-FHSTALSFYFTCKKPKI-----REKKLQHLLVDE-------GQTVQLECNADGDPQPVISWVTPRRRFITTKSNGRATV-------LGDGTLEIRFAQDQDSGMY---VCIASNAAG 490

[Close]

Query:  248 FQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNISTTSIXXXXXXXXXXXXXXXWNNNLKRLKFSSDYFKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIG--FHKMFPYIFQNLSYL---DASSNRIVKLSESMKYVLKFRQII-FSNNPLFCGCELNWLRNSNPKIKIKCSKVIQANDQSDIEVVENYQDPFYPKS-----HCYPPKTYLEIESAKKILKISIAEMTFDLYSCEFIQLRCIAVGDPLPTLEFIN-----FNEATFGKSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYG 1468
            F    +L  +++ KC +   P+    +L  L  +HL  LNI  +P   FK           HL NLE I  + L D       +L GLN+++ SI                N NL  + F +  FKH  +                       ++L +N IS                               + +G F+++ +L      I+G     + P+ FQ L +L   + S N +  L E++    +  +++  +NNPL C C L WL    P ++    + + A   +  E   +++D F+  +      C  PK        KK+  + + E        + +QL C A GDP P + ++          + G++++       +      IR +Q  ++  Y    C+ +N+ G
Sbjct:  173 FSGLLSLEQLTLEKCNLTAVPTEALSHLRSLIALHLKHLNINNMPVYAFKR--------LFHLKNLE-IDYWPLLDLMPAN--SLYGLNLTSLSIT---------------NTNLSTVPFLA--FKHLVY--------------------LTHLNLSYNPIST------------------------------IEAGMFSDLIRL--QELHIVGAQLRTIEPHSFQGLRFLRVLNVSQNLLETLEENVFSSPRALEVLSINNNPLACDCRLLWLLQRQPNLQFGGQQPMCAGPDTIRE--RSFKD-FHSTALSFYFTCKKPKI-----REKKLQHLLVDE-------GQTVQLECNADGDPQPVISWVTPRRRFITTKSNGRATV-------LGDGTLEIRFAQDQDSGMY---VCIASNAAG 490

[Close]

BLAST of SMED30024157 vs. UniProt/SwissProt
Match: sp|Q7L985|LIGO2_HUMAN (Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 2 OS=Homo sapiens OX=9606 GN=LINGO2 PE=2 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 5.375e-7
Identity = 91/426 (21.36%), Postives = 175/426 (41.08%), Query Frame = 2

Query:  248 FQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNIST--TSIXXXXXXXXXXXXXXXWNNNLKRLKFSSDY-FKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIG--FHKMFPYIFQNLSYL---DASSNRIVKLSESMKYVLKFRQII-FSNNPLFCGCELNWLRNSNPKIKIKCSKVIQANDQSDIEVVENYQDPFYPKS-----HCYPPKTYLEIESAKKILKISIAEMTFDLYSCEFIQLRCIAVGDPLPTLEFIN-----FNEATFGKSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYG 1468
            F    NL  + +   R+   P   F  LS L ++ ++E  I  + D +F++  N+K L  +   +L  IS  +      +E++ L   N++   T     L  +          NN+    F   +  KH + D   L+     NS +  NL +  V    N   +   +  +KHL+  T +     N+ +     + +G F+++ +L      I+G     + P+ FQ L +L   + S N +  L E++    +  +++  +NNPL C C L W+    P ++    + + A   +  E   +++D F+  +      C  PK        KK+  + + E        + +QL C A GDP P + ++          + G++++       +      IR +Q  ++  Y    C+ +N+ G
Sbjct:  101 FNNLFNLRSLRLKGNRLKLVPLGVFTGLSNLTKLDISENKIVILLDYMFQDLHNLKSLE-VGDNDLVYISHRAFSGLLSLEQLTLEKCNLTAVPTEALSHLRSLISLHLKHLNINNMPVYAFKRLFHLKHLEIDYWPLLDMMPANSLYGLNLTSLSVT---NTNLSTVPFLAFKHLVYLTHL-----NLSYNPISTIEAGMFSDLIRL--QELHIVGAQLRTIEPHSFQGLRFLRVLNVSQNLLETLEENVFSSPRALEVLSINNNPLACDCRLLWILQRQPTLQFGGQQPMCAGPDTIRE--RSFKD-FHSTALSFYFTCKKPKI-----REKKLQHLLVDE-------GQTVQLECSADGDPQPVISWVTPRRRFITTKSNGRATV-------LGDGTLEIRFAQDQDSGMY---VCIASNAAG 490

[Close]

BLAST of SMED30024157 vs. UniProt/SwissProt
Match: sp|Q3URE9|LIGO2_MOUSE (Leucine-rich repeat and immunoglobulin-like domain-containing nogo receptor-interacting protein 2 OS=Mus musculus OX=10090 GN=Lingo2 PE=2 SV=1)

HSP 1 Score: 53.9138 bits (128), Expect = 3.338e-6
Identity = 90/423 (21.28%), Postives = 159/423 (37.59%), Query Frame = 2

Query:  248 FQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNISTTSIXXXXXXXXXXXXXXXWNNNLKRLKFSSDYFKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIG--FHKMFPYIFQNLSYL---DASSNRIVKLSESMKYVLKFRQII-FSNNPLFCGCELNWLRNSNPKIKIKCSKVIQANDQSDIEVVENYQDPFYPKS-----HCYPPKTYLEIESAKKILKISIAEMTFDLYSCEFIQLRCIAVGDPLPTLEFIN-----FNEATFGKSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYG 1468
            F    +L  +++ KC +   P+    +L  L  +HL  LNI  +P   FK           HL NLE I  + L D       +L GLN+++ SI                N NL  + F +  FKH  +                       ++L +N IS                               + +G F+++ +L      I+G     + P+ FQ L +L   + S N +  L E++    +  +++  +NNPL C C L WL    P ++    + + A   +  E   +++D F+  +      C  PK        KK+  + + E        + +QL C A GDP P + ++          + G++++       +      IR +Q  ++  Y    C+ +N+ G
Sbjct:  173 FSGLLSLEQLTLEKCNLTAVPTEALSHLRSLIALHLKHLNINNMPVYAFKR--------LFHLKNLE-IDYWPLLDLMPAN--SLYGLNLTSLSIT---------------NTNLSTVPFLA--FKHLVY--------------------LTHLNLSYNPIST------------------------------IEAGMFSDLIRL--QELHIVGAQLRTIEPHSFQGLRFLRVLNVSQNLLETLEENVFSSPRALEVLSINNNPLACDCRLLWLLQRQPNLQFGGQQPMCAGPDTIRE--RSFKD-FHSTALSFYFTCKKPKI-----REKKLQHLLVDE-------GQTVQLECNADGDPQPVISWVTPRRRFITTKSNGRATV-------LGDGTLEIRFAQDQDSGMY---VCIASNAAG 490

[Close]

BLAST of SMED30024157 vs. TrEMBL
Match: T1DF80 (LRFN-1 OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 1.174e-12
Identity = 117/439 (26.65%), Postives = 174/439 (39.64%), Query Frame = 2

Query:  245 DFQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNISTTSIXXXXXXXXXXXXXXXWNN-NLKRLKFSSDYFKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEIS--NNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIGFHKMFPYIFQNLSYLDASSNRIVKLSESMK-YVLKFRQIIFSNNPLFCGCELNWLRNSNPKIKI-----------KCSKVIQANDQSDIEVVENYQDPFYPKSHCYPP-KTYLEIESAKKILKISI-------------AEMTFDLYSCEFIQLRCIAVGDPLPTLEFINFNEATFGKSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYGKS 1474
            DF++  +L  I I  C I       F    +L  V L  L +  + +  FK+S N+  LS I+L NLE I    L +  +   + L  + IST  +   L  +  T++ L+W + N + L      F  ++   L L  N+I N  F    ++ ++ L  N +S  +NF    Y  L   T+VL + +  I G FP   S     +  L + +N I   +     +F NL + D S NRI  L   +  +  K        NPL C CE+ WL  +  K KI           K   +   N   DI   +   D       C+PP    L I   KK    ++              + T  +   + I L C  +GDP+PTL + N       +S LN    IT   S        LS + K     C  +N  G S
Sbjct:   82 DFEQLESLNSIIIKSCPIETISEKLFSKKEYLGSVTLENLPLRHLRNIFFKSS-NLIELSLINLYNLEYIDSHILENQNKFSRLKLKNVTISTDVLQTVLSPIKNTIRELSWTDVNHQILVIQPMLFSSYKLGLLDLSNNKIENWNFLIYTKSRKILLK-NTVSYTSNFLLSKYASLPQFTQVLDLENIGIFGQFPSNISKPMIFLKTLNVKSNLIDSLNDNIDQMFPNLLFFDISLNRIKHLQSKLNNFFYKTEYFKLEGNPLHCNCEIRWLF-ARYKSKIQDFPRCHFGFKKSESINNRNSLKDIYFSQLSTDSL----ACFPPLPPVLSISKGKKSTMFTVNSTDPQTNSSTISKQYTLVMDPHQRIDLSCETLGDPIPTLTWSNKKGKVLHRSVLN----ITTFNSAVNTVTVGLSASEKSLVYMCTASNIEGVS 509

[Close]

BLAST of SMED30024157 vs. TrEMBL
Match: R7TAS3 (Ig-like domain-containing protein (Fragment) OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_52911 PE=4 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 6.850e-11
Identity = 98/422 (23.22%), Postives = 183/422 (43.36%), Query Frame = 2

Query:  248 FQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNISTTSIXXXXXXXXXXXXXXXWNNNLKRLKFSSDYFKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIGFHKMFPYIFQNLSYLDASSNRIVKLSESMKYV-LKFRQIIFSNNPLFCGCELNWLR---NSNPKIKIKCSKVIQANDQSDIEVVENYQDPFYPKSHCYPPKTYLEIESAKKILKISIAEMTFDLYSCEFIQLRCIAVGDPLPTLEFINFNEATFGKSSLNFYDEITIATSRKT--IRHSQLSENSKYNQLHCLGTNSYGKSIIRLQLN 1495
            F     L+ + +   +I Q  +  F  L  L+ + L + +IT I +  F+  +N+ RL+      L  IS  +     +VE++ +   ++S+ +    L+ +  +L+ L W NN K L+F+++ F  F F  L+L  N I + +F E +   E+ LD N I    D+  Y +L    K+     ++ +  F +V + +F ++T                     +LSYL    N I  LSE ++ + +  + ++   N L C CEL WL+    +   + I   + ++    S +  + +  D ++    C  P     I +  + L +S  E T          L CI  GDP PT+ + + N      +S+      T+  ++    IR + L +  +Y    C   N  G + +++ +N
Sbjct:   96 FSGLIKLITLDLRFNKIQQLQAYLFSGLIKLQTLRLEDNDITSIENFAFQ-GLNLTRLNLDRNEKLRDISGKAFHST-KVEQLRISNSSLSSET-AEALVPLKDSLRELHWQNNQKPLRFATEAFVGFTFRALKLNNNGIKDIDFLEGVVTDELSLDDNPIG-PIDFSRYPNLRQVRKL-----HLSNTSFSIVLASYFNDMT---------------------HLSYLGLEDNGISSLSEDLRPIFMGLQYLLLKGNTLHCNCELRWLKRWLTAESGVNINNGEALECVTPS-VRTITSMDDVYF---TCSEPV----IVTISRTLNVSEEEGT---------SLTCIGEGDPAPTIIWSSPNGDV---ASIPPPHSRTLTENQGIFHIRSASLHDRGEY---ACTAVNLAGNNTVKVHVN 464

[Close]

BLAST of SMED30024157 vs. TrEMBL
Match: A0A1I8IQ97 (Ig-like domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.228e-9
Identity = 88/392 (22.45%), Postives = 154/392 (39.29%), Query Frame = 2

Query:  275 ISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNISTTSIXXXXXXXXXXXXXXXWNNNL---KRLKFSSDYFKHFQ-FDELRLIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLIS-----DTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIGFHKMFPYIFQNLSYLDASSNRIVKLSESMKYVL-------KFR-QIIFSNNPLFCGCELNW------LRNSNPKIKIKCSKVIQANDQSDIEVVENYQDPFYPKSH---CYPP-----KTYLEIESAKK----ILKISIAEMTFD---------LYSCEFIQLRCIAVGDPLPTLEFI 1318
            IS+  C I + P   F  L  L  V L  L    +P  +F++S +++ L      +  + S   L +   V ++ L G  +    +   +  +   L  L++NN       L+  +  F +    +EL L  NQ+ +  F E + A+ + L +N +     W               + L + H  +   FPL     F  + +L + NN I           + L+ LD  SN+I +L   +K  +       +F+  +  ++NP+ C CEL+W      L NS PK  + C+   +   +  I          + + H   C PP     + ++ +  +KK    +L         D         L +    QL C + GDP PTL ++
Sbjct:   79 ISVHNCPIVELPDELFAYLPLLSSVILRNLPFATLPPRLFRDSPSLRTLWLTQTPHYSRQSGRPLANTI-VSDLRLTGTGLDGAGLLDLIAPIAGRLHKLSFNNRASLAGPLRLPAGAFNNTSSLEELNLSNNQLTDFGFLEGVSASRLLLSYNPVG----WPGELSFAGLRPNWRPQALELNHAGLQ-RFPLGVETAFPALQELLLANNVIKELPAEPLLALKRLTQLDVGSNQIRRLGSELKPAMLALLYRDRFKGHVYLADNPVHCNCELSWIFDSAVLHNSLPK-NVLCASGGELGGKLLI----------WAQPHQLLCLPPSKPGIQVHIAVSKSKKPTFDMLNFQPVGNGADPLSDVQQHQLPAGSTAQLICSSDGDPAPTLRWL 453

[Close]

BLAST of SMED30024157 vs. TrEMBL
Match: I1ZII9 (Leucine rich repeat and fibronectin type III domain containing-1 (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 8.516e-8
Identity = 63/267 (23.60%), Postives = 115/267 (43.07%), Query Frame = 2

Query:  581 NNNLKRLKFSSDYFKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNE-ISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIGFHKMFPYIFQNLSYLDASSNRIVKLSESMKYVLKFRQII-FSNNPLFCGCELNWLRNSNPKIKIKCSKVIQAN---DQSDIEVVENYQDPFYPKSH-----CYPPKT-YLEIESAKKILKISIA-------------EMTFDLYSCEFIQLRCIAVGDPLPTL 1309
            NN ++ +K    +F  +Q   L +  N   + +F    ++ ++ L  ++ + ++FD   +  L    ++  + +  IHG FP   S  F ++  L + +N I   H     +F +L  LD S N+I  L   +  +    +I     NPL C C++ WL   + + K K     + N     S++       D F+ +       C PP+   L I  A+  +K++++             E T  + S + ++L C   GDP PTL
Sbjct:   75 NNRIEIVKLPKRFFLDYQLGLLDVQNNLFLDWDFLTVTKSRKLLLKNSQALKSSFDLSKFSILYLYCELFDLENIGIHGTFPSTISEPFQHVKSLTVKSNSIESLHNSIDQMFPHLQLLDISYNKIKHLQPDLNNLFYKTEIFKLEGNPLHCNCQVRWL---SARYKSKIRDFPRCNFGYRNSNLSESTQSDDLFFSQLATDSLICLPPRAPILSISKAEVPIKLTVSGKDKPNNISIVKKEYTLVMNSNQLVELNCETEGDPTPTL 338

[Close]

BLAST of SMED30024157 vs. TrEMBL
Match: A0A2D1QA84 (Leucine rich repeat and Ig domain containing 2 (Fragment) OS=Emydura macquarii OX=90392 GN=lingo2 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 2.778e-7
Identity = 92/430 (21.40%), Postives = 170/430 (39.53%), Query Frame = 2

Query:  248 FQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNISTTSIXXXXXXXXXXXXXXXWNNNLKRLKFSSDYFKHFQF-DELRLIRNQIGNSE---FFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVH------------SGFFANITKLFINNNQIIGFHKMFPYIFQNLSY---LDASSNRIVKLSESMKYVLKFRQII-FSNNPLFCGCELNWLRNSNPKIKIKCSKVIQANDQSDIEVVENYQDPFYPKSHCYPPKTYLEIESAK---KILKISIAEMTFDLYSCEFIQLRCIAVGDPLPTLEFINFNEATFGKSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYG 1468
            F    +L  +++ KC +   P+    +L  L  +HL  LNI  +P   FK    +K L         +I+ + L D       +L GLN+++ S+                N NL  + +S+   KH  +   L L  N I   E   F + ++  E+H+   ++   +        +++T + +I ++ +  +  L H            +G FA++ +L   +        + P+ FQ L +   L+ S N +  L E++ +  K  +I+  +NNPL C C L W+    P ++    + + A         +N ++  +   H      Y   +  K   K L+  + E        + +QL C A GDP PT+ ++          S        +      IR +Q  +   Y    C+ +N+ G
Sbjct:  147 FSGLLSLEQLTLEKCNLTAVPTEALSHLHNLISLHLRHLNINLLPAYAFKRLFRLKDL---------EINYWPLLDMMPAN--SLYGLNLTSLSVT---------------NTNLSAIPYSA--LKHLVYLTHLNLSFNPISTIEAGMFADLVRLQELHMVGAQLRTIYGLNLTSLSVTNTNLSAIPYSALKHLVYLTHLNLSFNPISTIEAGMFADLVRLQALHMVGAQLRTIEPHAFQGLRFLRVLNVSQNLLETLEENVFHSPKTLEILCINNNPLACDCRLLWILQRQPTLQFGSQEPMCAGP-------DNVREKPFKDFHSTALSFYFTCKKPKIRDKKLQYLVVE------EGQTVQLMCNADGDPQPTISWVTPRRRLITTKSNG--RATVLGDGMLEIRFAQDQDTGIYT---CIASNAAG 530

[Close]

BLAST of SMED30024157 vs. Ensembl Medaka
Match: lrfn1 (leucine rich repeat and fibronectin type III domain containing 1 [Source:NCBI gene;Acc:101167138])

HSP 1 Score: 56.6102 bits (135), Expect = 5.732e-8
Identity = 80/338 (23.67%), Postives = 127/338 (37.57%), Query Frame = 2

Query:  614 DYFKHFQFDELRLIRNQIGN--SEFFENLQAAE-VHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIGF-----------------HKMFPYI-------FQNLSYLDASSNRIVKL------------SESMKYVLKFRQIIFSNNPLFCGCELNWLRNSNPKIKIKCSKVIQANDQSDIEVVENYQDPFYPKSH-----CYPPKTYLEIESAKKILKISIAEMTFDLYSCEFIQLRCIAVGDPLPTLEFINFNEATFGKSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYGKSIIRLQLN 1495
            D+        L L RN I       F  L++   +H+D N + N     ++K LI + + L +G+N IH V       F + I  L ++NN + G                  H +  +I          L  LD +SNR+ KL            SE          + F  NPL C CEL WLR           ++ + +D       E+  D ++         C PP     + S   +++             + + L+C AVGDP P   FI++ ++  GK   N    I        I  S L +N  YN   C+ +N+ G +   +++ 
Sbjct:   70 DFVNMTSLVHLTLSRNTISQIVPHAFVGLRSLRALHMDGNRL-NIIKSDHFKGLI-NLRHLILGNNQIHEVASTSFDEFVSTIEDLDLSNNNLRGLPWEAIARMSNINTLTLDHNLIDHIEAGTFTLLTKLVRLDMTSNRLQKLPPDSLFQHAQVLSEVKGSSSSTLAVSFGGNPLHCNCELLWLR-----------RLTREDDLETCASPEHLMDKYFWSIQEEEFICEPPLITKHVASKSYVME------------GQGVTLKCKAVGDPDP---FIHW-QSPDGKLVHNNSRTILYDNGTLDILISTLKDNGAYN---CVASNAAGIATAAVEIT 375

[Close]

BLAST of SMED30024157 vs. Ensembl Medaka
Match: chad (chondroadherin [Source:NCBI gene;Acc:105356591])

HSP 1 Score: 54.6842 bits (130), Expect = 1.258e-7
Identity = 79/328 (24.09%), Postives = 151/328 (46.04%), Query Frame = 2

Query:  104 CVTSKNFEIHQTECTSIPDENDLLRIPPDINYLIIKCNYSSKIKKIFDFQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLAN--LEKISPFSLRDCFQVEEINLGGLNISTTSIXXXXXXXXXXXXXXXWNNNLKRLK---FSSD---YFKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIGFHKMFPYIFQNLS---YLDASSNRIVKLSESMKYVLKFRQIIFSNNPLFCGCELNWLR 1054
            C+     +  Q++C   PD+ D L   P++ ++I  C+ +  +K+I      +NL  +++ +  +   P+  F     L  +H+    +TEI    FK    +K+L++++L+N  +  I P +  D  ++  + L G  IS  +  +F  +++     L+ NN ++ L+   FS      + H   ++L+ +  Q G+ +  ENL  A  HLD N  S        K  + +   L++G N +  +       F   + +L ++N   +G  K     F  ++    L   +NR+  L +S+K+       +F NNP  C C+L+ LR
Sbjct:   10 CLLGAAVQEAQSQC---PDQCDCL---PNLQHVI--CD-NKGLKEIPQVAESTNL--LNLQRNSLGSIPAGAFSESKGLISLHMQHCQLTEIGSQAFKG---LKKLTYLYLSNNDISSIKPGAFEDLTKLTYLYLDGNQISRLAKGIFSPMINLFSLQLS-NNKMRELRTGTFSGAKNLRWLHLNKNDLQTL--QPGSLDDVENL--AVFHLDGNNFSTYPSAAMSKLRVVEE--LTLGGNPMKTIPDNAFQSFGRYMERLHLDN---MGLEKFSDDAFNGVTAIKLLHLDNNRLKALPKSLKFGTITNMTLF-NNPWNCSCQLHSLR 312

[Close]

BLAST of SMED30024157 vs. Planmine SMEST
Match: SMESG000051501.1 (SMESG000051501.1)

HSP 1 Score: 1022.69 bits (2643), Expect = 0.000e+0
Identity = 540/540 (100.00%), Postives = 540/540 (100.00%), Query Frame = 2

Query:   41 MKFVIVIYYILLSNTFSDATTCVTSKNFEIHQTECTSIPDENDLLRIPPDINYLIIKCNYSSKIKKIFDFQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNISTTSIXXXXXXXXXXXXXXXWNNNLKRLKFSSDYFKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIGFHKMFPYIFQNLSYLDASSNRIVKLSESMKYVLKFRQIIFSNNPLFCGCELNWLRNSNPKIKIKCSKVIQANDQSDIEVVENYQDPFYPKSHCYPPKTYLEIESAKKILKISIAEMTFDLYSCEFIQLRCIAVGDPLPTLEFINFNEATFGKSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYGKSIIRLQLNKMYXXXXXXXXXXXXXXXXFCRERSSRLTANSLKVTFLFFLPMLINFIIENDQFL 1660
            MKFVIVIYYILLSNTFSDATTCVTSKNFEIHQTECTSIPDENDLLRIPPDINYLIIKCNYSSKIKKIFDFQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNISTTSIHLFLLVVHKTLKVLTWNNNLKRLKFSSDYFKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIGFHKMFPYIFQNLSYLDASSNRIVKLSESMKYVLKFRQIIFSNNPLFCGCELNWLRNSNPKIKIKCSKVIQANDQSDIEVVENYQDPFYPKSHCYPPKTYLEIESAKKILKISIAEMTFDLYSCEFIQLRCIAVGDPLPTLEFINFNEATFGKSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYGKSIIRLQLNKMYGKRKSKKSIEGIKKSKFCRERSSRLTANSLKVTFLFFLPMLINFIIENDQFL
Sbjct:    1 MKFVIVIYYILLSNTFSDATTCVTSKNFEIHQTECTSIPDENDLLRIPPDINYLIIKCNYSSKIKKIFDFQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNISTTSIHLFLLVVHKTLKVLTWNNNLKRLKFSSDYFKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIGFHKMFPYIFQNLSYLDASSNRIVKLSESMKYVLKFRQIIFSNNPLFCGCELNWLRNSNPKIKIKCSKVIQANDQSDIEVVENYQDPFYPKSHCYPPKTYLEIESAKKILKISIAEMTFDLYSCEFIQLRCIAVGDPLPTLEFINFNEATFGKSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYGKSIIRLQLNKMYGKRKSKKSIEGIKKSKFCRERSSRLTANSLKVTFLFFLPMLINFIIENDQFL 540

[Close]

BLAST of SMED30024157 vs. Planmine SMEST
Match: SMESG000051501.1 (SMESG000051501.1)

HSP 1 Score: 931.398 bits (2406), Expect = 0.000e+0
Identity = 495/496 (99.80%), Postives = 495/496 (99.80%), Query Frame = 2

Query:  173 LRIPPDINYLIIKCNYSSKIKKIFDFQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNISTTSIXXXXXXXXXXXXXXXWNNNLKRLKFSSDYFKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIGFHKMFPYIFQNLSYLDASSNRIVKLSESMKYVLKFRQIIFSNNPLFCGCELNWLRNSNPKIKIKCSKVIQANDQSDIEVVENYQDPFYPKSHCYPPKTYLEIESAKKILKISIAEMTFDLYSCEFIQLRCIAVGDPLPTLEFINFNEATFGKSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYGKSIIRLQLNKMYXXXXXXXXXXXXXXXXFCRERSSRLTANSLKVTFLFFLPMLINFIIENDQFL 1660
            L IPPDINYLIIKCNYSSKIKKIFDFQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNISTTSIHLFLLVVHKTLKVLTWNNNLKRLKFSSDYFKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIGFHKMFPYIFQNLSYLDASSNRIVKLSESMKYVLKFRQIIFSNNPLFCGCELNWLRNSNPKIKIKCSKVIQANDQSDIEVVENYQDPFYPKSHCYPPKTYLEIESAKKILKISIAEMTFDLYSCEFIQLRCIAVGDPLPTLEFINFNEATFGKSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYGKSIIRLQLNKMYGKRKSKKSIEGIKKSKFCRERSSRLTANSLKVTFLFFLPMLINFIIENDQFL
Sbjct:   27 LGIPPDINYLIIKCNYSSKIKKIFDFQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNISTTSIHLFLLVVHKTLKVLTWNNNLKRLKFSSDYFKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIGFHKMFPYIFQNLSYLDASSNRIVKLSESMKYVLKFRQIIFSNNPLFCGCELNWLRNSNPKIKIKCSKVIQANDQSDIEVVENYQDPFYPKSHCYPPKTYLEIESAKKILKISIAEMTFDLYSCEFIQLRCIAVGDPLPTLEFINFNEATFGKSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYGKSIIRLQLNKMYGKRKSKKSIEGIKKSKFCRERSSRLTANSLKVTFLFFLPMLINFIIENDQFL 522

[Close]

BLAST of SMED30024157 vs. Planmine SMEST
Match: SMESG000051501.1 (SMESG000051501.1)

HSP 1 Score: 929.472 bits (2401), Expect = 0.000e+0
Identity = 494/494 (100.00%), Postives = 494/494 (100.00%), Query Frame = 2

Query:  179 IPPDINYLIIKCNYSSKIKKIFDFQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNISTTSIXXXXXXXXXXXXXXXWNNNLKRLKFSSDYFKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIGFHKMFPYIFQNLSYLDASSNRIVKLSESMKYVLKFRQIIFSNNPLFCGCELNWLRNSNPKIKIKCSKVIQANDQSDIEVVENYQDPFYPKSHCYPPKTYLEIESAKKILKISIAEMTFDLYSCEFIQLRCIAVGDPLPTLEFINFNEATFGKSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYGKSIIRLQLNKMYXXXXXXXXXXXXXXXXFCRERSSRLTANSLKVTFLFFLPMLINFIIENDQFL 1660
            IPPDINYLIIKCNYSSKIKKIFDFQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNISTTSIHLFLLVVHKTLKVLTWNNNLKRLKFSSDYFKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIGFHKMFPYIFQNLSYLDASSNRIVKLSESMKYVLKFRQIIFSNNPLFCGCELNWLRNSNPKIKIKCSKVIQANDQSDIEVVENYQDPFYPKSHCYPPKTYLEIESAKKILKISIAEMTFDLYSCEFIQLRCIAVGDPLPTLEFINFNEATFGKSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYGKSIIRLQLNKMYGKRKSKKSIEGIKKSKFCRERSSRLTANSLKVTFLFFLPMLINFIIENDQFL
Sbjct:    5 IPPDINYLIIKCNYSSKIKKIFDFQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNISTTSIHLFLLVVHKTLKVLTWNNNLKRLKFSSDYFKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIGFHKMFPYIFQNLSYLDASSNRIVKLSESMKYVLKFRQIIFSNNPLFCGCELNWLRNSNPKIKIKCSKVIQANDQSDIEVVENYQDPFYPKSHCYPPKTYLEIESAKKILKISIAEMTFDLYSCEFIQLRCIAVGDPLPTLEFINFNEATFGKSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYGKSIIRLQLNKMYGKRKSKKSIEGIKKSKFCRERSSRLTANSLKVTFLFFLPMLINFIIENDQFL 498

[Close]

BLAST of SMED30024157 vs. Planmine SMEST
Match: SMESG000080200.1 (SMESG000080200.1)

HSP 1 Score: 134.035 bits (336), Expect = 3.676e-33
Identity = 140/512 (27.34%), Postives = 231/512 (45.12%), Query Frame = 2

Query:   47 FVIVIYYILLSNTFSDATT-----CVTSKNFEIH-QTECTSIPDENDLLRIPPDINYLIIKCNYSSKIKKIFD--FQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNISTTSIXXXXXXXXXXXXXXXWNNNLKRLKFSSDYFKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEIS--NNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIGFHKMFPYI---FQNLSYLDASSNRIVKLSESMKYVL--KFRQIIFSNNPLFCGCELNWLRN-SNPKIK-IKCSKVIQANDQSDIEVVENYQDPFYPKSHCYPPKTYLEIESAKKILKISIAEMT-------FDLYSCEFIQLRCIAVGDPLPTLEFINFNEATFG--KSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYGKSIIRLQLNKMY 1504
            F+I ++  LL    S  TT         +NF    ++ C+ IP + +  RI  +     I+C +S+K   + D   +    LV+I I  C +   P + F++L+ L  V+L  L I  + ++ FKNS N+  L    L +L++I P  L D  ++  + L   NI  T +   LL V+ TL    W  N++ L    DYF+ F F+ + L  N I N++F E L   E+ L  N I      +WKNY+ L    K L +    +     L     F  + +L +++N   G H++  ++     NL  LD  +N++  L  S+ Y+L  KF ++    N   C CE+ W+R+    +IK +KC  V       + E+  +       K  C  P   L++    K    +I+ ++       FDLY    I  +CI  G+P P L++I  N+ T       L+  +  T+  +       + ++N  Y    C   +  G S IRL++ K Y
Sbjct:   44 FLIEMWIYLLIGAISVVTTDQEGLVCNRQNFNNKPESHCSDIPSKEEWERIK-NTTIQTIRCKHSNKSNSLSDIRMKNLQRLVYIYIGDCNVQSLPMNFFEDLTQLETVYLDNLPIEILKENTFKNSSNIIELILSGLGSLKRIHPRFLGDQNKLATLTLTKSNIDPTQLRPTLLTVYNTLAKFYWRRNVQNLIIGKDYFQPFNFESVILEYNGITNTDFLELLNTKELSLKGNPIGIKEKLNWKNYRKLSKSLKKLDLSETGLD----LRPDMKFPKLEELALSSN---GIHQIPSHVEKFLPNLQLLDIRNNKLTTLP-SLDYMLRYKFGKVYLQGNIFNCNCEMIWIRSIEADRIKGLKCDNVNFEKHVKNQELTRSRI--LKSKMICTEPLLKLQLSDQFKSYDYTISVVSDTLQIYKFDLYPNHLISWQCIGEGNPAPRLKWIK-NQQTLKIENPPLDRLNNKTVMKNLIIPDDIKTADNETY---VCRAKSIAGISEIRLEVTKKY 540

[Close]

BLAST of SMED30024157 vs. Planmine SMEST
Match: SMESG000080200.1 (SMESG000080200.1)

HSP 1 Score: 132.88 bits (333), Expect = 6.231e-33
Identity = 131/477 (27.46%), Postives = 218/477 (45.70%), Query Frame = 2

Query:  134 QTECTSIPDENDLLRIPPDINYLIIKCNYSSKIKKIFD--FQRFSNLVFISISKCRINQFPSSTFQNLSFLREVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVEEINLGGLNISTTSIXXXXXXXXXXXXXXXWNNNLKRLKFSSDYFKHFQFDELRLIRNQIGNSEFFENLQAAEVHLDFNEIS--NNFDWKNYKHLISDTKVLSIGHNIIHGVFPLVHSGFFANITKLFINNNQIIGFHKMFPYI---FQNLSYLDASSNRIVKLSESMKYVL--KFRQIIFSNNPLFCGCELNWLRN-SNPKIK-IKCSKVIQANDQSDIEVVENYQDPFYPKSHCYPPKTYLEIESAKKILKISIAEMT-------FDLYSCEFIQLRCIAVGDPLPTLEFINFNEATFG--KSSLNFYDEITIATSRKTIRHSQLSENSKYNQLHCLGTNSYGKSIIRLQLNKMY 1504
            ++ C+ IP + +  RI  +     I+C +S+K   + D   +    LV+I I  C +   P + F++L+ L  V+L  L I  + ++ FKNS N+  L    L +L++I P  L D  ++  + L   NI  T +   LL V+ TL    W  N++ L    DYF+ F F+ + L  N I N++F E L   E+ L  N I      +WKNY+ L    K L +    +     L     F  + +L +++N   G H++  ++     NL  LD  +N++  L  S+ Y+L  KF ++    N   C CE+ W+R+    +IK +KC  V       + E+  +       K  C  P   L++    K    +I+ ++       FDLY    I  +CI  G+P P L++I  N+ T       L+  +  T+  +       + ++N  Y    C   +  G S IRL++ K Y
Sbjct:   32 ESHCSDIPSKEEWERIK-NTTIQTIRCKHSNKSNSLSDIRMKNLQRLVYIYIGDCNVQSLPMNFFEDLTQLETVYLDNLPIEILKENTFKNSSNIIELILSGLGSLKRIHPRFLGDQNKLATLTLTKSNIDPTQLRPTLLTVYNTLAKFYWRRNVQNLIIGKDYFQPFNFESVILEYNGITNTDFLELLNTKELSLKGNPIGIKEKLNWKNYRKLSKSLKKLDLSETGLD----LRPDMKFPKLEELALSSN---GIHQIPSHVEKFLPNLQLLDIRNNKLTTLP-SLDYMLRYKFGKVYLQGNIFNCNCEMIWIRSIEADRIKGLKCDNVNFEKHVKNQELTRSRI--LKSKMICTEPLLKLQLSDQFKSYDYTISVVSDTLQIYKFDLYPNHLISWQCIGEGNPAPRLKWIK-NQQTLKIENPPLDRLNNKTVMKNLIIPDDIKTADNETY---VCRAKSIAGISEIRLEVTKKY 493

The following BLAST results are available for this feature:

BLAST of SMED30024157 vs. Ensembl Human
BLAST of SMED30024157 vs. Ensembl Celegans
BLAST of SMED30024157 vs. Ensembl Fly
BLAST of SMED30024157 vs. Ensembl Zebrafish
BLAST of SMED30024157 vs. Ensembl Xenopus
BLAST of SMED30024157 vs. Ensembl Mouse
BLAST of SMED30024157 vs. UniProt/SwissProt
BLAST of SMED30024157 vs. TrEMBL
BLAST of SMED30024157 vs. Ensembl Cavefish
BLAST of SMED30024157 vs. Ensembl Sea Lamprey
BLAST of SMED30024157 vs. Ensembl Yeast
BLAST of SMED30024157 vs. Ensembl Nematostella
BLAST of SMED30024157 vs. Ensembl Medaka
BLAST of SMED30024157 vs. Planmine SMEST

BLAST of SMED30024157 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3


Match Name	E-value	Identity	Description
LINGO2	1.119e-7	21.36	leucine rich repeat and Ig domain containing 2 [So...	[more]
LINGO2	1.119e-7	21.36	leucine rich repeat and Ig domain containing 2 [So...	[more]
LINGO2	1.119e-7	21.36	leucine rich repeat and Ig domain containing 2 [So...	[more]

BLAST of SMED30024157 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30024157 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30024157 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30024157 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1


Match Name	E-value	Identity	Description
LINGO2	4.026e-6	20.90	leucine rich repeat and Ig domain containing 2 [So...	[more]

BLAST of SMED30024157 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Lingo2	4.772e-7	21.28	leucine rich repeat and Ig domain containing 2 [So...	[more]
Lingo2	4.772e-7	21.28	leucine rich repeat and Ig domain containing 2 [So...	[more]
Lingo2	4.772e-7	21.28	leucine rich repeat and Ig domain containing 2 [So...	[more]
Lingo2	4.772e-7	21.28	leucine rich repeat and Ig domain containing 2 [So...	[more]
Lingo2	4.772e-7	21.28	leucine rich repeat and Ig domain containing 2 [So...	[more]

BLAST of SMED30024157 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2


Match Name	E-value	Identity	Description
sp\|Q7L985\|LIGO2_HUMAN	5.375e-7	21.36	Leucine-rich repeat and immunoglobulin-like domain...	[more]
sp\|Q3URE9\|LIGO2_MOUSE	3.338e-6	21.28	Leucine-rich repeat and immunoglobulin-like domain...	[more]

BLAST of SMED30024157 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
T1DF80	1.174e-12	26.65	LRFN-1 OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1	[more]
R7TAS3	6.850e-11	23.22	Ig-like domain-containing protein (Fragment) OS=Ca...	[more]
A0A1I8IQ97	1.228e-9	22.45	Ig-like domain-containing protein OS=Macrostomum l...	[more]
I1ZII9	8.516e-8	23.60	Leucine rich repeat and fibronectin type III domai...	[more]
A0A2D1QA84	2.778e-7	21.40	Leucine rich repeat and Ig domain containing 2 (Fr...	[more]

BLAST of SMED30024157 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30024157 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30024157 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30024157 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of SMED30024157 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2


Match Name	E-value	Identity	Description
lrfn1	5.732e-8	23.67	leucine rich repeat and fibronectin type III domai...	[more]
chad	1.258e-7	24.09	chondroadherin [Source:NCBI gene;Acc:105356591]	[more]

BLAST of SMED30024157 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000051501.1	0.000e+0	100.00	SMESG000051501.1	[more]
SMESG000051501.1	0.000e+0	99.80	SMESG000051501.1	[more]
SMESG000051501.1	0.000e+0	100.00	SMESG000051501.1	[more]
SMESG000080200.1	3.676e-33	27.34	SMESG000080200.1	[more]
SMESG000080200.1	6.231e-33	27.46	SMESG000080200.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30024157 ID=SMED30024157|Name=SMED30024157|organism=Schmidtea mediterranea sexual|type=transcript|length=1667bp
TTTTATTTATTAGTTTTCTACATAATATTGTTTTTCTACAATGAAATTTG
TGATCGTTATTTACTATATTTTACTCTCAAATACATTCTCAGATGCGACT
ACCTGCGTTACATCAAAAAATTTTGAAATACATCAAACTGAATGTACCTC
AATTCCAGATGAAAACGATTTATTACGTATTCCTCCAGACATTAATTATC
TAATCATTAAATGCAATTATTCCAGCAAAATTAAAAAAATATTCGATTTC
CAGAGATTTTCAAATCTTGTGTTTATATCGATCTCAAAGTGCCGGATCAA
TCAATTTCCTTCATCAACATTTCAAAATTTATCATTTTTAAGAGAAGTTC
ATCTGGCCGAGCTCAATATTACTGAAATACCGGACGATATCTTTAAAAAT
TCAGTGAACGTTAAACGATTGTCTTTCATTCATCTAGCCAACCTCGAGAA
AATTTCTCCATTCTCTCTTAGAGATTGTTTTCAGGTCGAAGAAATAAATC
TTGGGGGTTTGAACATATCAACTACTTCCATCCATCTATTCTTACTTGTC
GTCCATAAAACCTTGAAGGTTTTGACGTGGAATAACAATTTGAAAAGGCT
CAAATTCAGTTCAGACTACTTTAAGCACTTTCAATTCGATGAATTGAGAT
TAATTCGTAACCAGATTGGCAATTCCGAATTTTTCGAAAATCTCCAAGCT
GCCGAAGTTCATTTGGATTTCAATGAAATAAGTAATAATTTTGACTGGAA
AAATTACAAACATTTAATAAGCGACACAAAAGTGCTCAGCATTGGACATA
ATATTATTCACGGAGTTTTCCCATTGGTCCACAGCGGATTTTTTGCGAAC
ATTACAAAGCTATTTATCAACAACAATCAAATCATCGGATTTCATAAAAT
GTTTCCATATATCTTTCAAAATCTGTCCTATTTAGACGCAAGTTCAAATA
GAATAGTAAAACTGTCAGAATCCATGAAATATGTTCTGAAATTCAGGCAA
ATTATCTTTTCTAATAATCCTTTGTTTTGTGGATGTGAACTGAACTGGTT
GCGGAACTCGAATCCGAAAATTAAAATCAAATGTTCCAAAGTAATTCAGG
CGAATGATCAGTCAGATATTGAGGTCGTAGAAAATTATCAAGACCCCTTC
TACCCTAAATCTCATTGTTACCCACCAAAAACATATTTAGAAATCGAAAG
CGCCAAAAAGATTTTGAAAATTTCAATTGCGGAAATGACTTTCGACCTGT
ACTCCTGTGAGTTTATTCAATTAAGATGTATAGCTGTAGGAGATCCCTTA
CCGACTTTAGAATTTATCAATTTTAATGAAGCAACATTTGGCAAATCTTC
ATTAAATTTTTATGACGAGATAACAATTGCAACTTCCCGTAAAACTATCA
GACATTCGCAATTATCAGAGAATTCCAAATATAATCAACTTCATTGTCTT
GGAACCAACTCGTACGGGAAGTCCATAATTCGACTCCAATTGAACAAGAT
GTATGGTAAACGTAAGTCTAAAAAGTCCATCGAAGGTATAAAAAAATCAA
AATTCTGTCGTGAAAGATCTTCAAGATTGACTGCTAATAGCCTGAAAGTC
ACATTCTTATTTTTTCTTCCGATGTTGATAAATTTTATTATTGAAAATGA
CCAATTTTTGTAATAAA

protein sequence of SMED30024157-orf-1

>SMED30024157-orf-1 ID=SMED30024157-orf-1|Name=SMED30024157-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=541bp
MKFVIVIYYILLSNTFSDATTCVTSKNFEIHQTECTSIPDENDLLRIPPD
INYLIIKCNYSSKIKKIFDFQRFSNLVFISISKCRINQFPSSTFQNLSFL
REVHLAELNITEIPDDIFKNSVNVKRLSFIHLANLEKISPFSLRDCFQVE
EINLGGLNISTTSIHLFLLVVHKTLKVLTWNNNLKRLKFSSDYFKHFQFD
ELRLIRNQIGNSEFFENLQAAEVHLDFNEISNNFDWKNYKHLISDTKVLS
IGHNIIHGVFPLVHSGFFANITKLFINNNQIIGFHKMFPYIFQNLSYLDA
SSNRIVKLSESMKYVLKFRQIIFSNNPLFCGCELNWLRNSNPKIKIKCSK
VIQANDQSDIEVVENYQDPFYPKSHCYPPKTYLEIESAKKILKISIAEMT
FDLYSCEFIQLRCIAVGDPLPTLEFINFNEATFGKSSLNFYDEITIATSR
KTIRHSQLSENSKYNQLHCLGTNSYGKSIIRLQLNKMYGKRKSKKSIEGI
KKSKFCRERSSRLTANSLKVTFLFFLPMLINFIIENDQFL*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000420	parapharyngeal region
PLANA:0002032	epidermal cell
PLANA:0002056	medial muscle cell
PLANA:0003104	enteric muscle cell
PLANA:0003116	parenchymal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR000421	FA58C
IPR032675	LRR_dom_sf
IPR001611	Leu-rich_rpt

Vocabulary: **molecular function**
Term	Definition
GO:0005515	protein binding
GO:0004860	protein kinase inhibitor activity
GO:0005057	signal transducer activity, downstream of receptor
GO:0005104	fibroblast growth factor receptor binding
GO:0030674	protein binding, bridging
GO:0042803	protein homodimerization activity
GO:0045499	chemorepellent activity

Vocabulary: **biological process**
Term	Definition
GO:0003345	proepicardium cell migration involved in pericardium morphogenesis
GO:0006469	negative regulation of protein kinase activity
GO:0007155	cell adhesion
GO:0007275	multicellular organism development
GO:0007411	axon guidance
GO:0007416	synapse assembly
GO:0007507	heart development
GO:0008543	fibroblast growth factor receptor signaling pathway
GO:0019221	cytokine-mediated signaling pathway
GO:0031175	neuron projection development
GO:0046426	negative regulation of JAK-STAT cascade
GO:0048598	embryonic morphogenesis
GO:0048678	response to axon injury
GO:0050919	negative chemotaxis
GO:0051965	positive regulation of synapse assembly
GO:0060322	head development
GO:0098742	cell-cell adhesion via plasma-membrane adhesion molecules
GO:1990138	neuron projection extension

Vocabulary: **cellular component**
Term	Definition
GO:0005576	extracellular region
GO:0005578	proteinaceous extracellular matrix
GO:0005615	extracellular space
GO:0005737	cytoplasm
GO:0005783	endoplasmic reticulum
GO:0005789	endoplasmic reticulum membrane
GO:0005886	plasma membrane
GO:0005887	integral component of plasma membrane
GO:0005911	cell-cell junction
GO:0005925	focal adhesion
GO:0016020	membrane
GO:0016021	integral component of membrane
GO:0030054	cell junction
GO:0030424	axon
GO:0042995	cell projection
GO:0043679	axon terminus
GO:0044295	axonal growth cone
GO:0097060	synaptic membrane

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032675	Leucine-rich repeat domain superfamily	GENE3D	G3DSA:3.80.10.10		coord: 217..365 e-value: 4.1E-12 score: 47.7
IPR032675	Leucine-rich repeat domain superfamily	GENE3D	G3DSA:3.80.10.10		coord: 13..216 e-value: 2.6E-17 score: 64.4
None	No IPR available	PANTHER	PTHR24373	SLIT RELATED LEUCINE-RICH REPEAT NEURONAL PROTEIN	coord: 19..438
None	No IPR available	SIGNALP_EUK	SignalP-noTM	SignalP-noTM	coord: 1..19 score: 0.619
None	No IPR available	SUPERFAMILY	SSF52058	L domain-like	coord: 17..340
IPR001611	Leucine-rich repeat	PROSITE	PS51450	LRR	coord: 294..315 score: 7.019
IPR001611	Leucine-rich repeat	PROSITE	PS51450	LRR	coord: 99..120 score: 4.793
IPR001611	Leucine-rich repeat	PROSITE	PS51450	LRR	coord: 75..96 score: 4.693
IPR001611	Leucine-rich repeat	PROSITE	PS51450	LRR	coord: 198..219 score: 4.716

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SMED30024157

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 5

Owlery

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