C2H2-type domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30024108 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Homology
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A4E0RUS9 (Zinc finger protein OS=Fasciola hepatica OX=6192 GN=D915_003077 PE=4 SV=1) HSP 1 Score: 57.3806 bits (137), Expect = 2.365e-15 Identity = 25/40 (62.50%), Postives = 28/40 (70.00%), Query Frame = 1 Query: 1105 VHSFGRPFSCYICSKSYKWIWDLGRHFHKNHSQFDNPYRK 1224 VHS RP C IC SYKW WDLGRHF K+H + NPY+K Sbjct: 321 VHSKERPHRCVICGSSYKWKWDLGRHFDKSHQKNMNPYKK 360 HSP 2 Score: 55.0694 bits (131), Expect = 2.365e-15 Identity = 46/160 (28.75%), Postives = 73/160 (45.62%), Query Frame = 2 Query: 653 QYLNQNNETTNIVEQEGFCKPCQQQEALDLSIRK----TDQINQIAKINVKKFNQKIILKSEREHRKENVNVITCAMCKYTFLTMSQLNRHFGMYHEDFVIKYSYNLQHSRSKLTFQAHLASLRCLNQTKSIDKL-----QCPSCQYRAKWPTELEKHVM 1105 +Y N + + T+ +E+ F Q++ + +R+ T QIN+ +K V K V C CK F T+ +LN HF + H F+++ L+H++S + ++ QT L CP C Y AKWPTEL+KH+M Sbjct: 183 RYPNTDEKCTSPLEK--FSSSRTQRKRPNTGLRRARNSTTQINKASKTGVGK-----------------QPVTQCHQCKVLFPTLMELNSHFLVEHA-FILRT--ELEHTKSWKSHALETMYIQSDPQTAIRGGLLSTGYPCPYCDYFAKWPTELQKHIM 320
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A504Z0N1 (Zinc finger protein OS=Fasciola gigantica OX=46835 GN=FGIG_09392 PE=4 SV=1) HSP 1 Score: 57.3806 bits (137), Expect = 2.444e-15 Identity = 25/40 (62.50%), Postives = 28/40 (70.00%), Query Frame = 1 Query: 1105 VHSFGRPFSCYICSKSYKWIWDLGRHFHKNHSQFDNPYRK 1224 VHS RP C IC SYKW WDLGRHF K+H + NPY+K Sbjct: 321 VHSKERPHRCVICGSSYKWKWDLGRHFDKSHQKNMNPYKK 360 HSP 2 Score: 55.0694 bits (131), Expect = 2.444e-15 Identity = 46/160 (28.75%), Postives = 73/160 (45.62%), Query Frame = 2 Query: 653 QYLNQNNETTNIVEQEGFCKPCQQQEALDLSIRK----TDQINQIAKINVKKFNQKIILKSEREHRKENVNVITCAMCKYTFLTMSQLNRHFGMYHEDFVIKYSYNLQHSRSKLTFQAHLASLRCLNQTKSIDKL-----QCPSCQYRAKWPTELEKHVM 1105 +YLN + + T+ +E+ F Q++ + +R+ T QIN+ +K V K V C CK F + +LN HF + H F+++ L+H++S + ++ QT L CP C Y AKWPTEL+KH+M Sbjct: 183 RYLNTDEKCTSPLEK--FSSSRTQRKRPNTGLRRARNSTTQINKTSKTGVGK-----------------QPVTQCHQCKVLFPNLMELNSHFLVEHA-FILRT--ELEHTKSWKSHALETMYIQSDPQTAIRGGLLSTGYPCPYCDYFAKWPTELQKHIM 320
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A1S6KMJ7 (C2H2-type domain-containing protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 55.8398 bits (133), Expect = 7.086e-15 Identity = 32/92 (34.78%), Postives = 51/92 (55.43%), Query Frame = 2 Query: 854 VITCAMCKYTFLTMSQLNRHFGMYHEDFVIKYSYNLQHSRSKLTFQAHLASLRCLNQTKSIDK--------LQCPSCQYRAKWPTELEKHVM 1105 V +C C +F ++ LN HF HE +IK L+ ++S +++H C+ + +++ K QCP C+Y AKWPTEL+KH+M Sbjct: 214 VTSCLQCSSSFASLIDLNTHFLNEHELCLIK---ELELAKS---WKSHAVEEICMQELEAVVKQGEIETTGYQCPHCEYFAKWPTELQKHIM 299 HSP 2 Score: 54.6842 bits (130), Expect = 7.086e-15 Identity = 24/40 (60.00%), Postives = 27/40 (67.50%), Query Frame = 1 Query: 1105 VHSFGRPFSCYICSKSYKWIWDLGRHFHKNHSQFDNPYRK 1224 VHS RP C IC +YKW WDLGRHF K+H NPY+K Sbjct: 300 VHSKERPHQCIICGLTYKWKWDLGRHFDKSHLPAVNPYKK 339
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: A0A5K4FDC1 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 56.6102 bits (135), Expect = 1.466e-14 Identity = 25/40 (62.50%), Postives = 29/40 (72.50%), Query Frame = 1 Query: 1105 VHSFGRPFSCYICSKSYKWIWDLGRHFHKNHSQFDNPYRK 1224 VHS RP C IC SYKW WDLGRHF K+H++ NPY+K Sbjct: 418 VHSKQRPHRCIICGLSYKWKWDLGRHFDKSHNRTMNPYKK 457 HSP 2 Score: 53.1434 bits (126), Expect = 1.466e-14 Identity = 30/93 (32.26%), Postives = 48/93 (51.61%), Query Frame = 2 Query: 854 VITCAMCKYTFLTMSQLNRHFGMYHEDFVIKYSYNLQHSRSKLTFQAHLASLRCLNQTK---------SIDKLQCPSCQYRAKWPTELEKHVM 1105 V C C F ++ +LN HF H D +++ ++H++S +++H CL + ++ CPSC Y AKWPTEL+KH+M Sbjct: 331 VTRCNHCHVLFPSLYELNNHFIKEH-DVILQAE--MEHTKS---WKSHTIDDSCLQNMRILMNRSTKMNMSGYPCPSCDYVAKWPTELQKHIM 417
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Match: G4LWG3 (Zinc finger protein, putative OS=Schistosoma mansoni OX=6183 GN=Smp_125970 PE=4 SV=1) HSP 1 Score: 56.6102 bits (135), Expect = 2.317e-14 Identity = 25/40 (62.50%), Postives = 29/40 (72.50%), Query Frame = 1 Query: 1105 VHSFGRPFSCYICSKSYKWIWDLGRHFHKNHSQFDNPYRK 1224 VHS RP C IC SYKW WDLGRHF K+H++ NPY+K Sbjct: 135 VHSKQRPHRCIICGLSYKWKWDLGRHFDKSHNRTMNPYKK 174 HSP 2 Score: 52.373 bits (124), Expect = 2.317e-14 Identity = 30/93 (32.26%), Postives = 48/93 (51.61%), Query Frame = 2 Query: 854 VITCAMCKYTFLTMSQLNRHFGMYHEDFVIKYSYNLQHSRSKLTFQAHLASLRCLNQTK---------SIDKLQCPSCQYRAKWPTELEKHVM 1105 V C C F ++ +LN HF H D +++ ++H++S +++H CL + ++ CPSC Y AKWPTEL+KH+M Sbjct: 48 VTRCNHCHVLFPSLYELNNHFIKEH-DVILQAE--MEHTKS---WKSHTIDDSCLQNMRILMNRSTKMNMSGYPCPSCDYVAKWPTELQKHIM 134
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Match: SMESG000010914.1 (SMESG000010914.1) HSP 1 Score: 390.963 bits (1003), Expect = 9.424e-136 Identity = 188/188 (100.00%), Postives = 188/188 (100.00%), Query Frame = 2 Query: 542 MDLSKHLLLNQKFLNPNLDPMMILDTYRSFMQLQEIYQYLNQNNETTNIVEQEGFCKPCQQQEALDLSIRKTDQINQIAKINVKKFNQKIILKSEREHRKENVNVITCAMCKYTFLTMSQLNRHFGMYHEDFVIKYSYNLQHSRSKLTFQAHLASLRCLNQTKSIDKLQCPSCQYRAKWPTELEKHVM 1105 MDLSKHLLLNQKFLNPNLDPMMILDTYRSFMQLQEIYQYLNQNNETTNIVEQEGFCKPCQQQEALDLSIRKTDQINQIAKINVKKFNQKIILKSEREHRKENVNVITCAMCKYTFLTMSQLNRHFGMYHEDFVIKYSYNLQHSRSKLTFQAHLASLRCLNQTKSIDKLQCPSCQYRAKWPTELEKHVM Sbjct: 1 MDLSKHLLLNQKFLNPNLDPMMILDTYRSFMQLQEIYQYLNQNNETTNIVEQEGFCKPCQQQEALDLSIRKTDQINQIAKINVKKFNQKIILKSEREHRKENVNVITCAMCKYTFLTMSQLNRHFGMYHEDFVIKYSYNLQHSRSKLTFQAHLASLRCLNQTKSIDKLQCPSCQYRAKWPTELEKHVM 188 HSP 2 Score: 109.383 bits (272), Expect = 4.126e-27 Identity = 49/49 (100.00%), Postives = 49/49 (100.00%), Query Frame = 1 Query: 1105 VHSFGRPFSCYICSKSYKWIWDLGRHFHKNHSQFDNPYRKTSRVKSKVV 1251 VHSFGRPFSCYICSKSYKWIWDLGRHFHKNHSQFDNPYRKTSRVKSKVV Sbjct: 189 VHSFGRPFSCYICSKSYKWIWDLGRHFHKNHSQFDNPYRKTSRVKSKVV 237
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Match: SMESG000004948.1 (SMESG000004948.1) HSP 1 Score: 56.9954 bits (136), Expect = 1.458e-18 Identity = 38/121 (31.40%), Postives = 60/121 (49.59%), Query Frame = 2 Query: 767 NQIAKINVKKFNQKIILKSEREHRKENVNVITCAMCKYTFLTMSQLNRHFGMYHEDFVIKYSYNLQHSRSKLTFQAHLASLRCLNQTKSIDK--------LQCPSCQYRAKWPTELEKHVM 1105 N+ +KI N + S R V +C C +F ++ LN HF HE +IK L+ ++S +++H C+ + +++ K QCP C+Y AKWPTEL+KH+M Sbjct: 188 NKASKIAT---NMTAVKSSRRTSGGGKQPVTSCLQCSSSFASLIDLNTHFLNEHELCLIK---ELELAKS---WKSHAVEEICMQELEAVVKQGEIETTGYQCPHCEYFAKWPTELQKHIM 299 HSP 2 Score: 54.6842 bits (130), Expect = 1.458e-18 Identity = 24/40 (60.00%), Postives = 27/40 (67.50%), Query Frame = 1 Query: 1105 VHSFGRPFSCYICSKSYKWIWDLGRHFHKNHSQFDNPYRK 1224 VHS RP C IC +YKW WDLGRHF K+H NPY+K Sbjct: 300 VHSKERPHQCIICGLTYKWKWDLGRHFDKSHLPAVNPYKK 339 The following BLAST results are available for this feature:
BLAST of C2H2-type domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of C2H2-type domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of C2H2-type domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30024108 ID=SMED30024108|Name=C2H2-type domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1401bpback to top protein sequence of SMED30024108-orf-1 >SMED30024108-orf-1 ID=SMED30024108-orf-1|Name=SMED30024108-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=247bp MDLSKHLLLNQKFLNPNLDPMMILDTYRSFMQLQEIYQYLNQNNETTNIVback to top Annotated Terms
The following terms have been associated with this transcript:
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