SMED30024100

Overview
NameSMED30024100
Smed IDSMED30024100
Length (bp)509
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30024100 (SMED30024100) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30024100 (SMED30024100) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30024100

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
cephalic gangliaSMED30024100h1SMcG0017034 dd_Smed_v4_6570_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30024100h1SMcG0017034 dd_Smed_v4_6570_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30024100h1SMcG0017034 dd_Smed_v4_4737_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30024100h1SMcG0017034 dd_Smed_v4_4737_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30024100h1SMcG0017034 dd_Smed_v4_6570_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30024100h1SMcG0017034 dd_Smed_v4_4737_0_1dd_Smed_v4PMID:30962434
Cote et al., 2019
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30024100 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.004501:8521..12061 -2308
Homology
BLAST of SMED30024100 vs. Planmine SMEST
Match: SMESG000066354.1 (SMESG000066354.1)

HSP 1 Score: 126.331 bits (316), Expect = 6.327e-34
Identity = 136/137 (99.27%), Postives = 136/137 (99.27%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLC 509
            LCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPISTEPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMSTEPTICP MKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLC
Sbjct: 1062 LCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPISTEPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMSTEPTICPTMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLC 1198          

HSP 2 Score: 103.219 bits (256), Expect = 8.222e-26
Identity = 80/133 (60.15%), Postives = 93/133 (69.92%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX----------------------EPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKDLCTNPAV+VMCKKTC SST+   S+  +S SS P STEPTLCP MKDLC NPAVS++CKKTCAS+T+  S +S+  S                       EPT+CPAMKDLCTNPAVS+MCKKTCA
Sbjct: 1150 ICPTMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLCPAMKDLCTNPAVSIMCKKTCASTTTGISSASSGSSKSSTPLSTSSTSGSSVSSTPVSTEPTLCPAMKDLCTNPAVSIMCKKTCA 1282          

HSP 3 Score: 95.5153 bits (236), Expect = 3.303e-23
Identity = 75/116 (64.66%), Postives = 91/116 (78.45%), Query Frame = 3
Query:  102 CPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            CP MKDLC NP VA MCKKTCGS+T S  ST  S+ +STPISTEP+LCP++KDLC+NP+VS +CKKTC SST+A S  S        +STEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  313 CPNMKDLCINPTVASMCKKTCGSTTPSKSSTSGSTATSTPISTEPSLCPKLKDLCLNPSVSGVCKKTCGSSTTAKSSVSASSITSKPVSTEPTVCPDMKDMCTNPSVAKLCKKTCA 428          

HSP 4 Score: 93.9745 bits (232), Expect = 1.204e-22
Identity = 88/143 (61.54%), Postives = 108/143 (75.52%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLC 509
            +CP MKD+C NP++A +CKKTC SST S  ST  SS+SSTP STEPT+CP MKD+C NP+V+ +CKKTC S+T+  S ++         STEPT+CPAMKDLCTNPAV+VMCKKTC SST+   S+  +S SS P STEPTLC
Sbjct:  968 VCPDMKDMCINPSIAKLCKKTCASSTKSKSSTSGSSVSSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCGSTTTGKSSTNVSSVSSTPKSTEPTLCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPISTEPTLC 1110          

HSP 5 Score: 91.6633 bits (226), Expect = 9.397e-22
Identity = 91/143 (63.64%), Postives = 113/143 (79.02%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLC 509
            +CP MKD+CTNP+VA +CKKTC  ST+   S+  SSISST ISTEPT+CP MKD+C+NP+++ +CKKTCA      SSTS SS+SST  STEPT+CP MKD+CTNP+V+ +CKKTC S+TT KSS+  +S SS P STEPTLC
Sbjct:  921 VCPDMKDMCTNPSVAKLCKKTCAFSTTPKSSSRGSSISSTQISTEPTVCPDMKDMCINPSIAKLCKKTCASSTKSKSSTSGSSVSSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCGSTTTGKSSTNVSSVSSTPKSTEPTLC 1063          

HSP 6 Score: 89.7373 bits (221), Expect = 4.150e-21
Identity = 72/116 (62.07%), Postives = 89/116 (76.72%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXX-----XXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CKKTC SST+S  ST  SSISST ISTEPT+CP MKD+C NP+V+ +CK TCASST+  S +          TEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  773 VCPDMKDMCTNPSVAKLCKKTCASSTTSKSSTSGSSISSTQISTEPTVCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTKGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCA 888          

HSP 7 Score: 87.8113 bits (216), Expect = 1.601e-20
Identity = 68/117 (58.12%), Postives = 89/117 (76.07%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX------EPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CKKTC SST+   ST  SS SST +STEPT+CP MK++C NP+V+ +CKKTCAS+T+  S +S    +      EPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  679 VCPDMKDMCTNPSVAKLCKKTCASSTTQESSTSGSSKSSTSMSTEPTVCPDMKEMCTNPSVAKLCKKTCASTTTPKSSTSGSSISSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCA 795          

HSP 8 Score: 87.4261 bits (215), Expect = 2.471e-20
Identity = 73/125 (58.40%), Postives = 93/125 (74.40%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAX--------------XXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            LCP +KDLC NP+V+ +CKKTCGSST++  S   SSI+S P+STEPT+CP MKD+C NP+V+ +CKKTCA               STS SS+SST +STEPT+CP MKD+CTNP+V+ +CKKTC 
Sbjct:  359 LCPKLKDLCLNPSVSGVCKKTCGSSTTAKSSVSASSITSKPVSTEPTVCPDMKDMCTNPSVAKLCKKTCAFPTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCG 483          

HSP 9 Score: 81.6481 bits (200), Expect = 2.268e-18
Identity = 70/125 (56.00%), Postives = 89/125 (71.20%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX--------------EPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CK TC SST+   ST  SSISSTPI+ EPT+CP MKD+C NP+V+ +CKKTCASST+  S +S    +              EPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  820 VCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTKGSSISSTPIT-EPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCA 943          

HSP 10 Score: 81.2629 bits (199), Expect = 3.116e-18
Identity = 71/132 (53.79%), Postives = 88/132 (66.67%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXX--------XXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXX-------------XEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CKKTCGSST+   ST  SS SST          STEPT+CP MKD+C NP+V+ +CK TCASST+  S +S                 TEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  461 VCPDMKDMCTNPSVAKLCKKTCGSSTTPRSSTSGSSKSSTSGSSISSKPMSTEPTVCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTSESSKSSTSGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCA 592          

HSP 11 Score: 80.4925 bits (197), Expect = 6.057e-18
Identity = 73/132 (55.30%), Postives = 90/132 (68.18%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXX--------XXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXX-------------XEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CKKTC SST+   ST  SS SS        TPISTEPT+CP MKD+C NP+V+ +CKKTCASST+  S +S                 TEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  570 VCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGPSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCA 701          

HSP 12 Score: 78.9518 bits (193), Expect = 2.200e-17
Identity = 72/132 (54.55%), Postives = 90/132 (68.18%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXX-------XEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXX--------------XXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CK TC SST+   ST  SS SST  S       TEPT+CP MKD+C NP+V+ +CKKTCASST+  S +S              T +STEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  516 VCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTSESSKSSTSGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGPSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCA 647          

HSP 13 Score: 77.0258 bits (188), Expect = 8.714e-17
Identity = 63/116 (54.31%), Postives = 87/116 (75.00%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX-----XXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+C+   V+ +CKKTCGSSTS+  ST I++IS+TP+STEP++CP MKD+C  P +S++CKKTCA+S+   S S+       + TEPT CP MK+ C+NP V++MCKKTC 
Sbjct:   97 ICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLSTEPSVCPAMKDICNLPTISVMCKKTCATSSIGKSSSTFSISTTPIFTEPTQCPDMKNYCSNPGVALMCKKTCG 212          

HSP 14 Score: 75.8702 bits (185), Expect = 2.712e-16
Identity = 80/142 (56.34%), Postives = 102/142 (71.83%), Query Frame = 3
Query:  102 CPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLC 509
            CP MKD+C   +V+++CKKTC        ST +SS S TPISTEPT CP MKDLC+NP V+ +CKKTC S+T + S +S        +STEP++CP +KDLC NP+VS +CKKTC SSTT KSS +A+S +S P STEPT+C
Sbjct:  272 CPLMKDICHLQSVSIICKKTCA------SSTPMSSKSPTPISTEPTQCPNMKDLCINPTVASMCKKTCGSTTPSKSSTSGSTATSTPISTEPSLCPKLKDLCLNPSVSGVCKKTCGSSTTAKSSVSASSITSKPVSTEPTVC 407          

HSP 15 Score: 67.781 bits (164), Expect = 1.509e-13
Identity = 46/67 (68.66%), Postives = 51/67 (76.12%), Query Frame = 3
Query:  231 EPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            EPT CP MKD+C   +VS+ICKKTCASST  SS S T +STEPT CP MKDLC NP V+ MCKKTC 
Sbjct:  268 EPTQCPLMKDICHLQSVSIICKKTCASSTPMSSKSPTPISTEPTQCPNMKDLCINPTVASMCKKTCG 334          

HSP 16 Score: 67.0106 bits (162), Expect = 2.710e-13
Identity = 55/116 (47.41%), Postives = 70/116 (60.34%), Query Frame = 3
Query:  102 CPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXX------XXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            C  M  +C NP V+ +C KTC S+T ++  +            EPT+CP MKD+C    VS +CKKTC SSTS  S +S         STEP++CPAMKD+C  P +SVMCKKTCA
Sbjct:   61 CAFMTQICHNPGVSQICPKTCASNTFTSTESPT----------EPTICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLSTEPSVCPAMKDICNLPTISVMCKKTCA 166          

HSP 17 Score: 53.5286 bits (127), Expect = 1.450e-8
Identity = 35/67 (52.24%), Postives = 43/67 (64.18%), Query Frame = 3
Query:  231 EPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            EP  C  M  +C NP VS IC KTCA    +++ +ST   TEPTICP MKD+C+   VS +CKKTC 
Sbjct:   57 EPAQCAFMTQICHNPGVSQICPKTCA----SNTFTSTESPTEPTICPLMKDICSLETVSSLCKKTCG 119          
BLAST of SMED30024100 vs. Planmine SMEST
Match: SMESG000066354.1 (SMESG000066354.1)

HSP 1 Score: 126.331 bits (316), Expect = 6.327e-34
Identity = 136/137 (99.27%), Postives = 136/137 (99.27%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLC 509
            LCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPISTEPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMSTEPTICP MKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLC
Sbjct: 1063 LCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPISTEPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMSTEPTICPTMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLC 1199          

HSP 2 Score: 103.219 bits (256), Expect = 8.223e-26
Identity = 80/133 (60.15%), Postives = 93/133 (69.92%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX----------------------EPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKDLCTNPAV+VMCKKTC SST+   S+  +S SS P STEPTLCP MKDLC NPAVS++CKKTCAS+T+  S +S+  S                       EPT+CPAMKDLCTNPAVS+MCKKTCA
Sbjct: 1151 ICPTMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLCPAMKDLCTNPAVSIMCKKTCASTTTGISSASSGSSKSSTPLSTSSTSGSSVSSTPVSTEPTLCPAMKDLCTNPAVSIMCKKTCA 1283          

HSP 3 Score: 95.5153 bits (236), Expect = 3.367e-23
Identity = 75/116 (64.66%), Postives = 91/116 (78.45%), Query Frame = 3
Query:  102 CPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            CP MKDLC NP VA MCKKTCGS+T S  ST  S+ +STPISTEP+LCP++KDLC+NP+VS +CKKTC SST+A S  S        +STEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  313 CPNMKDLCINPTVASMCKKTCGSTTPSKSSTSGSTATSTPISTEPSLCPKLKDLCLNPSVSGVCKKTCGSSTTAKSSVSASSITSKPVSTEPTVCPDMKDMCTNPSVAKLCKKTCA 428          

HSP 4 Score: 93.9745 bits (232), Expect = 1.215e-22
Identity = 88/143 (61.54%), Postives = 108/143 (75.52%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLC 509
            +CP MKD+C NP++A +CKKTC SST S  ST  SS+SSTP STEPT+CP MKD+C NP+V+ +CKKTC S+T+  S ++         STEPT+CPAMKDLCTNPAV+VMCKKTC SST+   S+  +S SS P STEPTLC
Sbjct:  969 VCPDMKDMCINPSIAKLCKKTCASSTKSKSSTSGSSVSSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCGSTTTGKSSTNVSSVSSTPKSTEPTLCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPISTEPTLC 1111          

HSP 5 Score: 91.2781 bits (225), Expect = 9.581e-22
Identity = 91/143 (63.64%), Postives = 113/143 (79.02%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLC 509
            +CP MKD+CTNP+VA +CKKTC  ST+   S+  SSISST ISTEPT+CP MKD+C+NP+++ +CKKTCA      SSTS SS+SST  STEPT+CP MKD+CTNP+V+ +CKKTC S+TT KSS+  +S SS P STEPTLC
Sbjct:  922 VCPDMKDMCTNPSVAKLCKKTCAFSTTPKSSSRGSSISSTQISTEPTVCPDMKDMCINPSIAKLCKKTCASSTKSKSSTSGSSVSSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCGSTTTGKSSTNVSSVSSTPKSTEPTLC 1064          

HSP 6 Score: 89.7373 bits (221), Expect = 4.232e-21
Identity = 72/116 (62.07%), Postives = 89/116 (76.72%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXX-----XXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CKKTC SST+S  ST  SSISST ISTEPT+CP MKD+C NP+V+ +CK TCASST+  S +          TEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  774 VCPDMKDMCTNPSVAKLCKKTCASSTTSKSSTSGSSISSTQISTEPTVCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTKGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCA 889          

HSP 7 Score: 87.8113 bits (216), Expect = 1.632e-20
Identity = 68/117 (58.12%), Postives = 89/117 (76.07%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX------EPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CKKTC SST+   ST  SS SST +STEPT+CP MK++C NP+V+ +CKKTCAS+T+  S +S    +      EPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  680 VCPDMKDMCTNPSVAKLCKKTCASSTTQESSTSGSSKSSTSMSTEPTVCPDMKEMCTNPSVAKLCKKTCASTTTPKSSTSGSSISSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCA 796          

HSP 8 Score: 87.4261 bits (215), Expect = 2.519e-20
Identity = 73/125 (58.40%), Postives = 93/125 (74.40%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAX--------------XXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            LCP +KDLC NP+V+ +CKKTCGSST++  S   SSI+S P+STEPT+CP MKD+C NP+V+ +CKKTCA               STS SS+SST +STEPT+CP MKD+CTNP+V+ +CKKTC 
Sbjct:  359 LCPKLKDLCLNPSVSGVCKKTCGSSTTAKSSVSASSITSKPVSTEPTVCPDMKDMCTNPSVAKLCKKTCAFPTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCG 483          

HSP 9 Score: 81.6481 bits (200), Expect = 2.312e-18
Identity = 70/125 (56.00%), Postives = 89/125 (71.20%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX--------------EPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CK TC SST+   ST  SSISSTPI+ EPT+CP MKD+C NP+V+ +CKKTCASST+  S +S    +              EPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  821 VCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTKGSSISSTPIT-EPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCA 944          

HSP 10 Score: 81.2629 bits (199), Expect = 3.177e-18
Identity = 71/132 (53.79%), Postives = 88/132 (66.67%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXX--------XXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXX-------------XEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CKKTCGSST+   ST  SS SST          STEPT+CP MKD+C NP+V+ +CK TCASST+  S +S                 TEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  461 VCPDMKDMCTNPSVAKLCKKTCGSSTTPRSSTSGSSKSSTSGSSISSKPMSTEPTVCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTSESSKSSTSGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCA 592          

HSP 11 Score: 79.7221 bits (195), Expect = 1.029e-17
Identity = 72/133 (54.14%), Postives = 90/133 (67.67%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXX--------XXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX--------------EPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CKKTC SST+   ST  SS SS        TPISTEPT+CP MKD+C NP+V+ +CKKTCASST+  S +S    +              EPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  570 VCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGPSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCA 702          

HSP 12 Score: 78.9518 bits (193), Expect = 2.243e-17
Identity = 72/132 (54.55%), Postives = 90/132 (68.18%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXX-------XEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXX--------------XXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CK TC SST+   ST  SS SST  S       TEPT+CP MKD+C NP+V+ +CKKTCASST+  S +S              T +STEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  516 VCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTSESSKSSTSGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGPSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCA 647          

HSP 13 Score: 77.0258 bits (188), Expect = 8.548e-17
Identity = 63/116 (54.31%), Postives = 87/116 (75.00%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX-----XXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+C+   V+ +CKKTCGSSTS+  ST I++IS+TP+STEP++CP MKD+C  P +S++CKKTCA+S+   S S+       + TEPT CP MK+ C+NP V++MCKKTC 
Sbjct:   97 ICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLSTEPSVCPAMKDICNLPTISVMCKKTCATSSIGKSSSTFSISTTPIFTEPTQCPDMKNYCSNPGVALMCKKTCG 212          

HSP 14 Score: 75.8702 bits (185), Expect = 2.712e-16
Identity = 80/142 (56.34%), Postives = 102/142 (71.83%), Query Frame = 3
Query:  102 CPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLC 509
            CP MKD+C   +V+++CKKTC        ST +SS S TPISTEPT CP MKDLC+NP V+ +CKKTC S+T + S +S        +STEP++CP +KDLC NP+VS +CKKTC SSTT KSS +A+S +S P STEPT+C
Sbjct:  272 CPLMKDICHLQSVSIICKKTCA------SSTPMSSKSPTPISTEPTQCPNMKDLCINPTVASMCKKTCGSTTPSKSSTSGSTATSTPISTEPSLCPKLKDLCLNPSVSGVCKKTCGSSTTAKSSVSASSITSKPVSTEPTVC 407          

HSP 15 Score: 67.781 bits (164), Expect = 1.524e-13
Identity = 46/67 (68.66%), Postives = 51/67 (76.12%), Query Frame = 3
Query:  231 EPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            EPT CP MKD+C   +VS+ICKKTCASST  SS S T +STEPT CP MKDLC NP V+ MCKKTC 
Sbjct:  268 EPTQCPLMKDICHLQSVSIICKKTCASSTPMSSKSPTPISTEPTQCPNMKDLCINPTVASMCKKTCG 334          

HSP 16 Score: 67.0106 bits (162), Expect = 2.633e-13
Identity = 55/116 (47.41%), Postives = 70/116 (60.34%), Query Frame = 3
Query:  102 CPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXX------XXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            C  M  +C NP V+ +C KTC S+T ++  +            EPT+CP MKD+C    VS +CKKTC SSTS  S +S         STEP++CPAMKD+C  P +SVMCKKTCA
Sbjct:   61 CAFMTQICHNPGVSQICPKTCASNTFTSTESPT----------EPTICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLSTEPSVCPAMKDICNLPTISVMCKKTCA 166          

HSP 17 Score: 53.5286 bits (127), Expect = 1.450e-8
Identity = 35/67 (52.24%), Postives = 43/67 (64.18%), Query Frame = 3
Query:  231 EPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            EP  C  M  +C NP VS IC KTCA    +++ +ST   TEPTICP MKD+C+   VS +CKKTC 
Sbjct:   57 EPAQCAFMTQICHNPGVSQICPKTCA----SNTFTSTESPTEPTICPLMKDICSLETVSSLCKKTCG 119          
BLAST of SMED30024100 vs. Planmine SMEST
Match: SMESG000066354.1 (SMESG000066354.1)

HSP 1 Score: 126.331 bits (316), Expect = 7.049e-34
Identity = 136/137 (99.27%), Postives = 136/137 (99.27%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLC 509
            LCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPISTEPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMSTEPTICP MKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLC
Sbjct: 1061 LCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPISTEPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMSTEPTICPTMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLC 1197          

HSP 2 Score: 104.76 bits (260), Expect = 2.439e-26
Identity = 81/112 (72.32%), Postives = 93/112 (83.04%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXX-XXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            LCPAMKDLCTNPAV++MCKKTC S+T+   S    S  S+  +STEPTLCP MK LC NP V+ +CKKTCASSTS SS+SST +STEPT+CPAMKDLCTNPAVS+MCKKTCA
Sbjct: 1196 LCPAMKDLCTNPAVSIMCKKTCASTTTGISSASSGSSKSSTPLSTEPTLCPDMKMLCNNPNVAKMCKKTCASSTSGSSVSSTPVSTEPTLCPAMKDLCTNPAVSIMCKKTCA 1307          

HSP 3 Score: 95.5153 bits (236), Expect = 3.679e-23
Identity = 75/116 (64.66%), Postives = 91/116 (78.45%), Query Frame = 3
Query:  102 CPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            CP MKDLC NP VA MCKKTCGS+T S  ST  S+ +STPISTEP+LCP++KDLC+NP+VS +CKKTC SST+A S  S        +STEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  313 CPNMKDLCINPTVASMCKKTCGSTTPSKSSTSGSTATSTPISTEPSLCPKLKDLCLNPSVSGVCKKTCGSSTTAKSSVSASSITSKPVSTEPTVCPDMKDMCTNPSVAKLCKKTCA 428          

HSP 4 Score: 93.9745 bits (232), Expect = 1.183e-22
Identity = 88/142 (61.97%), Postives = 109/142 (76.76%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX-----XXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLC 509
            +CP MKD+C NP++A +CKKTC SST S  ST  SS+SSTP STEPT+CP MKD+C NP+V+ +CKKTC+++T  SS + +       STEPT+CPAMKDLCTNPAV+VMCKKTC SST+   S+  +S SS P STEPTLC
Sbjct:  968 VCPDMKDMCINPSIAKLCKKTCASSTKSKSSTSGSSVSSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCSTTTGKSSTNVSSVSSTPKSTEPTLCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPISTEPTLC 1109          

HSP 5 Score: 89.7373 bits (221), Expect = 4.078e-21
Identity = 74/117 (63.25%), Postives = 94/117 (80.34%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CKKTC  ST+   S+  SSISST ISTEPT+CP MKD+C+NP+++ +CKKTCA      SSTS SS+SST  STEPT+CP MKD+CTNP+V+ +CKKTC+
Sbjct:  921 VCPDMKDMCTNPSVAKLCKKTCAFSTTPKSSSRGSSISSTQISTEPTVCPDMKDMCINPSIAKLCKKTCASSTKSKSSTSGSSVSSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCS 1037          

HSP 6 Score: 89.3521 bits (220), Expect = 4.623e-21
Identity = 72/116 (62.07%), Postives = 89/116 (76.72%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXX-----XXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CKKTC SST+S  ST  SSISST ISTEPT+CP MKD+C NP+V+ +CK TCASST+  S +          TEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  773 VCPDMKDMCTNPSVAKLCKKTCASSTTSKSSTSGSSISSTQISTEPTVCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTKGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCA 888          

HSP 7 Score: 87.8113 bits (216), Expect = 1.800e-20
Identity = 68/117 (58.12%), Postives = 89/117 (76.07%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX------EPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CKKTC SST+   ST  SS SST +STEPT+CP MK++C NP+V+ +CKKTCAS+T+  S +S    +      EPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  679 VCPDMKDMCTNPSVAKLCKKTCASSTTQESSTSGSSKSSTSMSTEPTVCPDMKEMCTNPSVAKLCKKTCASTTTPKSSTSGSSISSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCA 795          

HSP 8 Score: 87.0409 bits (214), Expect = 2.832e-20
Identity = 73/125 (58.40%), Postives = 93/125 (74.40%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAX--------------XXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            LCP +KDLC NP+V+ +CKKTCGSST++  S   SSI+S P+STEPT+CP MKD+C NP+V+ +CKKTCA               STS SS+SST +STEPT+CP MKD+CTNP+V+ +CKKTC 
Sbjct:  359 LCPKLKDLCLNPSVSGVCKKTCGSSTTAKSSVSASSITSKPVSTEPTVCPDMKDMCTNPSVAKLCKKTCAFPTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCG 483          

HSP 9 Score: 81.6481 bits (200), Expect = 2.525e-18
Identity = 70/125 (56.00%), Postives = 89/125 (71.20%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX--------------EPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CK TC SST+   ST  SSISSTPI+ EPT+CP MKD+C NP+V+ +CKKTCASST+  S +S    +              EPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  820 VCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTKGSSISSTPIT-EPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCA 943          

HSP 10 Score: 81.2629 bits (199), Expect = 3.504e-18
Identity = 71/132 (53.79%), Postives = 88/132 (66.67%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXX--------XXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXX-------------XEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CKKTCGSST+   ST  SS SST          STEPT+CP MKD+C NP+V+ +CK TCASST+  S +S                 TEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  461 VCPDMKDMCTNPSVAKLCKKTCGSSTTPRSSTSGSSKSSTSGSSISSKPMSTEPTVCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTSESSKSSTSGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCA 592          

HSP 11 Score: 80.4925 bits (197), Expect = 6.810e-18
Identity = 73/132 (55.30%), Postives = 90/132 (68.18%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXX--------XXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXX-------------XEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CKKTC SST+   ST  SS SS        TPISTEPT+CP MKD+C NP+V+ +CKKTCASST+  S +S                 TEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  570 VCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGPSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCA 701          

HSP 12 Score: 78.9518 bits (193), Expect = 2.450e-17
Identity = 72/132 (54.55%), Postives = 90/132 (68.18%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXX-------XEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXX--------------XXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CK TC SST+   ST  SS SST  S       TEPT+CP MKD+C NP+V+ +CKKTCASST+  S +S              T +STEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  516 VCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTSESSKSSTSGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGPSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCA 647          

HSP 13 Score: 77.0258 bits (188), Expect = 9.609e-17
Identity = 63/116 (54.31%), Postives = 87/116 (75.00%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX-----XXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+C+   V+ +CKKTCGSSTS+  ST I++IS+TP+STEP++CP MKD+C  P +S++CKKTCA+S+   S S+       + TEPT CP MK+ C+NP V++MCKKTC 
Sbjct:   97 ICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLSTEPSVCPAMKDICNLPTISVMCKKTCATSSIGKSSSTFSISTTPIFTEPTQCPDMKNYCSNPGVALMCKKTCG 212          

HSP 14 Score: 75.485 bits (184), Expect = 3.048e-16
Identity = 80/142 (56.34%), Postives = 102/142 (71.83%), Query Frame = 3
Query:  102 CPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLC 509
            CP MKD+C   +V+++CKKTC        ST +SS S TPISTEPT CP MKDLC+NP V+ +CKKTC S+T + S +S        +STEP++CP +KDLC NP+VS +CKKTC SSTT KSS +A+S +S P STEPT+C
Sbjct:  272 CPLMKDICHLQSVSIICKKTCA------SSTPMSSKSPTPISTEPTQCPNMKDLCINPTVASMCKKTCGSTTPSKSSTSGSTATSTPISTEPSLCPKLKDLCLNPSVSGVCKKTCGSSTTAKSSVSASSITSKPVSTEPTVC 407          

HSP 15 Score: 67.781 bits (164), Expect = 1.586e-13
Identity = 46/67 (68.66%), Postives = 51/67 (76.12%), Query Frame = 3
Query:  231 EPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            EPT CP MKD+C   +VS+ICKKTCASST  SS S T +STEPT CP MKDLC NP V+ MCKKTC 
Sbjct:  268 EPTQCPLMKDICHLQSVSIICKKTCASSTPMSSKSPTPISTEPTQCPNMKDLCINPTVASMCKKTCG 334          

HSP 16 Score: 67.0106 bits (162), Expect = 2.902e-13
Identity = 55/116 (47.41%), Postives = 70/116 (60.34%), Query Frame = 3
Query:  102 CPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXX------XXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            C  M  +C NP V+ +C KTC S+T ++  +            EPT+CP MKD+C    VS +CKKTC SSTS  S +S         STEP++CPAMKD+C  P +SVMCKKTCA
Sbjct:   61 CAFMTQICHNPGVSQICPKTCASNTFTSTESPT----------EPTICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLSTEPSVCPAMKDICNLPTISVMCKKTCA 166          

HSP 17 Score: 53.1434 bits (126), Expect = 1.553e-8
Identity = 35/67 (52.24%), Postives = 43/67 (64.18%), Query Frame = 3
Query:  231 EPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            EP  C  M  +C NP VS IC KTCA    +++ +ST   TEPTICP MKD+C+   VS +CKKTC 
Sbjct:   57 EPAQCAFMTQICHNPGVSQICPKTCA----SNTFTSTESPTEPTICPLMKDICSLETVSSLCKKTCG 119          
BLAST of SMED30024100 vs. Planmine SMEST
Match: SMESG000066354.1 (SMESG000066354.1)

HSP 1 Score: 126.331 bits (316), Expect = 7.117e-34
Identity = 136/137 (99.27%), Postives = 136/137 (99.27%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLC 509
            LCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPISTEPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMSTEPTICP MKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLC
Sbjct: 1061 LCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPISTEPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMSTEPTICPTMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLC 1197          

HSP 2 Score: 104.76 bits (260), Expect = 2.486e-26
Identity = 81/112 (72.32%), Postives = 93/112 (83.04%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXX-XXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            LCPAMKDLCTNPAV++MCKKTC S+T+   S    S  S+  +STEPTLCP MK LC NP V+ +CKKTCASSTS SS+SST +STEPT+CPAMKDLCTNPAVS+MCKKTCA
Sbjct: 1196 LCPAMKDLCTNPAVSIMCKKTCASTTTGISSASSGSSKSSTPLSTEPTLCPDMKMLCNNPNVAKMCKKTCASSTSGSSVSSTPVSTEPTLCPAMKDLCTNPAVSIMCKKTCA 1307          

HSP 3 Score: 95.5153 bits (236), Expect = 3.715e-23
Identity = 75/116 (64.66%), Postives = 91/116 (78.45%), Query Frame = 3
Query:  102 CPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            CP MKDLC NP VA MCKKTCGS+T S  ST  S+ +STPISTEP+LCP++KDLC+NP+VS +CKKTC SST+A S  S        +STEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  313 CPNMKDLCINPTVASMCKKTCGSTTPSKSSTSGSTATSTPISTEPSLCPKLKDLCLNPSVSGVCKKTCGSSTTAKSSVSASSITSKPVSTEPTVCPDMKDMCTNPSVAKLCKKTCA 428          

HSP 4 Score: 93.9745 bits (232), Expect = 1.354e-22
Identity = 88/143 (61.54%), Postives = 108/143 (75.52%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLC 509
            +CP MKD+C NP++A +CKKTC SST S  ST  SS+SSTP STEPT+CP MKD+C NP+V+ +CKKTC S+T+  S ++         STEPT+CPAMKDLCTNPAV+VMCKKTC SST+   S+  +S SS P STEPTLC
Sbjct:  967 VCPDMKDMCINPSIAKLCKKTCASSTKSKSSTSGSSVSSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCGSTTTGKSSTNVSSVSSTPKSTEPTLCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPISTEPTLC 1109          

HSP 5 Score: 91.2781 bits (225), Expect = 1.088e-21
Identity = 91/143 (63.64%), Postives = 113/143 (79.02%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLC 509
            +CP MKD+CTNP+VA +CKKTC  ST+   S+  SSISST ISTEPT+CP MKD+C+NP+++ +CKKTCA      SSTS SS+SST  STEPT+CP MKD+CTNP+V+ +CKKTC S+TT KSS+  +S SS P STEPTLC
Sbjct:  920 VCPDMKDMCTNPSVAKLCKKTCAFSTTPKSSSRGSSISSTQISTEPTVCPDMKDMCINPSIAKLCKKTCASSTKSKSSTSGSSVSSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCGSTTTGKSSTNVSSVSSTPKSTEPTLC 1062          

HSP 6 Score: 89.3521 bits (220), Expect = 4.667e-21
Identity = 72/116 (62.07%), Postives = 89/116 (76.72%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXX-----XXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CKKTC SST+S  ST  SSISST ISTEPT+CP MKD+C NP+V+ +CK TCASST+  S +          TEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  772 VCPDMKDMCTNPSVAKLCKKTCASSTTSKSSTSGSSISSTQISTEPTVCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTKGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCA 887          

HSP 7 Score: 87.8113 bits (216), Expect = 1.667e-20
Identity = 67/124 (54.03%), Postives = 89/124 (71.77%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAX-------------XXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            LCP +KDLC NP+V+ +CKKTCGSST++  S   SSI+S P+STEPT+CP MKD+C NP+V+ +CKKTCA              S+++ S  S+   TEPT+CP MKD+CTNP+V+ +CKKTC 
Sbjct:  359 LCPKLKDLCLNPSVSGVCKKTCGSSTTAKSSVSASSITSKPVSTEPTVCPDMKDMCTNPSVAKLCKKTCAFPTTPKSSTSGSSKSSTSGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCG 482          

HSP 8 Score: 87.8113 bits (216), Expect = 1.818e-20
Identity = 68/117 (58.12%), Postives = 89/117 (76.07%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX------EPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CKKTC SST+   ST  SS SST +STEPT+CP MK++C NP+V+ +CKKTCAS+T+  S +S    +      EPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  678 VCPDMKDMCTNPSVAKLCKKTCASSTTQESSTSGSSKSSTSMSTEPTVCPDMKEMCTNPSVAKLCKKTCASTTTPKSSTSGSSISSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCA 794          

HSP 9 Score: 81.6481 bits (200), Expect = 2.550e-18
Identity = 70/125 (56.00%), Postives = 89/125 (71.20%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX--------------EPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CK TC SST+   ST  SSISSTPI+ EPT+CP MKD+C NP+V+ +CKKTCASST+  S +S    +              EPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  819 VCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTKGSSISSTPIT-EPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCA 942          

HSP 10 Score: 81.2629 bits (199), Expect = 3.504e-18
Identity = 71/132 (53.79%), Postives = 88/132 (66.67%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXX--------XXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXX-------------XEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CKKTCGSST+   ST  SS SST          STEPT+CP MKD+C NP+V+ +CK TCASST+  S +S                 TEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  460 VCPDMKDMCTNPSVAKLCKKTCGSSTTPRSSTSGSSKSSTSGSSISSKPMSTEPTVCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTSESSKSSTSGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCA 591          

HSP 11 Score: 80.4925 bits (197), Expect = 6.876e-18
Identity = 73/132 (55.30%), Postives = 90/132 (68.18%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXX--------XXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXX-------------XEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CKKTC SST+   ST  SS SS        TPISTEPT+CP MKD+C NP+V+ +CKKTCASST+  S +S                 TEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  569 VCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGPSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCA 700          

HSP 12 Score: 78.9518 bits (193), Expect = 2.474e-17
Identity = 72/132 (54.55%), Postives = 90/132 (68.18%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXX-------XEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXX--------------XXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CK TC SST+   ST  SS SST  S       TEPT+CP MKD+C NP+V+ +CKKTCASST+  S +S              T +STEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  515 VCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTSESSKSSTSGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGPSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCA 646          

HSP 13 Score: 77.0258 bits (188), Expect = 9.609e-17
Identity = 63/116 (54.31%), Postives = 87/116 (75.00%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX-----XXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+C+   V+ +CKKTCGSSTS+  ST I++IS+TP+STEP++CP MKD+C  P +S++CKKTCA+S+   S S+       + TEPT CP MK+ C+NP V++MCKKTC 
Sbjct:   97 ICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLSTEPSVCPAMKDICNLPTISVMCKKTCATSSIGKSSSTFSISTTPIFTEPTQCPDMKNYCSNPGVALMCKKTCG 212          

HSP 14 Score: 75.485 bits (184), Expect = 3.019e-16
Identity = 80/142 (56.34%), Postives = 102/142 (71.83%), Query Frame = 3
Query:  102 CPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLC 509
            CP MKD+C   +V+++CKKTC        ST +SS S TPISTEPT CP MKDLC+NP V+ +CKKTC S+T + S +S        +STEP++CP +KDLC NP+VS +CKKTC SSTT KSS +A+S +S P STEPT+C
Sbjct:  272 CPLMKDICHLQSVSIICKKTCA------SSTPMSSKSPTPISTEPTQCPNMKDLCINPTVASMCKKTCGSTTPSKSSTSGSTATSTPISTEPSLCPKLKDLCLNPSVSGVCKKTCGSSTTAKSSVSASSITSKPVSTEPTVC 407          

HSP 15 Score: 67.781 bits (164), Expect = 1.586e-13
Identity = 46/67 (68.66%), Postives = 51/67 (76.12%), Query Frame = 3
Query:  231 EPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            EPT CP MKD+C   +VS+ICKKTCASST  SS S T +STEPT CP MKDLC NP V+ MCKKTC 
Sbjct:  268 EPTQCPLMKDICHLQSVSIICKKTCASSTPMSSKSPTPISTEPTQCPNMKDLCINPTVASMCKKTCG 334          

HSP 16 Score: 67.0106 bits (162), Expect = 2.959e-13
Identity = 55/116 (47.41%), Postives = 70/116 (60.34%), Query Frame = 3
Query:  102 CPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXX------XXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            C  M  +C NP V+ +C KTC S+T ++  +            EPT+CP MKD+C    VS +CKKTC SSTS  S +S         STEP++CPAMKD+C  P +SVMCKKTCA
Sbjct:   61 CAFMTQICHNPGVSQICPKTCASNTFTSTESPT----------EPTICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLSTEPSVCPAMKDICNLPTISVMCKKTCA 166          

HSP 17 Score: 53.1434 bits (126), Expect = 1.538e-8
Identity = 35/67 (52.24%), Postives = 43/67 (64.18%), Query Frame = 3
Query:  231 EPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            EP  C  M  +C NP VS IC KTCA    +++ +ST   TEPTICP MKD+C+   VS +CKKTC 
Sbjct:   57 EPAQCAFMTQICHNPGVSQICPKTCA----SNTFTSTESPTEPTICPLMKDICSLETVSSLCKKTCG 119          
BLAST of SMED30024100 vs. Planmine SMEST
Match: SMESG000066354.1 (SMESG000066354.1)

HSP 1 Score: 126.331 bits (316), Expect = 7.176e-34
Identity = 136/137 (99.27%), Postives = 136/137 (99.27%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLC 509
            LCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPISTEPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMSTEPTICP MKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLC
Sbjct: 1066 LCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPISTEPTLCPQMKDLCMNPAVSLICKKTCASSTSASSLSSTIMSTEPTICPTMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLC 1202          

HSP 2 Score: 103.219 bits (256), Expect = 8.885e-26
Identity = 80/133 (60.15%), Postives = 93/133 (69.92%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX----------------------EPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKDLCTNPAV+VMCKKTC SST+   S+  +S SS P STEPTLCP MKDLC NPAVS++CKKTCAS+T+  S +S+  S                       EPT+CPAMKDLCTNPAVS+MCKKTCA
Sbjct: 1154 ICPTMKDLCTNPAVSVMCKKTCASSTTLKSSSTATSQSSVPTSTEPTLCPAMKDLCTNPAVSIMCKKTCASTTTGISSASSGSSKSSTPLSTSSTSGSSVSSTPVSTEPTLCPAMKDLCTNPAVSIMCKKTCA 1286          

HSP 3 Score: 95.5153 bits (236), Expect = 3.569e-23
Identity = 75/116 (64.66%), Postives = 91/116 (78.45%), Query Frame = 3
Query:  102 CPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            CP MKDLC NP VA MCKKTCGS+T S  ST  S+ +STPISTEP+LCP++KDLC+NP+VS +CKKTC SST+A S  S        +STEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  316 CPNMKDLCINPTVASMCKKTCGSTTPSKSSTSGSTATSTPISTEPSLCPKLKDLCLNPSVSGVCKKTCGSSTTAKSSVSASSITSKPVSTEPTVCPDMKDMCTNPSVAKLCKKTCA 431          

HSP 4 Score: 93.9745 bits (232), Expect = 1.251e-22
Identity = 88/143 (61.54%), Postives = 108/143 (75.52%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLC 509
            +CP MKD+C NP++A +CKKTC SST S  ST  SS+SSTP STEPT+CP MKD+C NP+V+ +CKKTC S+T+  S ++         STEPT+CPAMKDLCTNPAV+VMCKKTC SST+   S+  +S SS P STEPTLC
Sbjct:  972 VCPDMKDMCINPSIAKLCKKTCASSTKSKSSTSGSSVSSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCGSTTTGKSSTNVSSVSSTPKSTEPTLCPAMKDLCTNPAVAVMCKKTCGSSTSSTLSTFISSISSTPISTEPTLC 1114          

HSP 5 Score: 91.2781 bits (225), Expect = 9.864e-22
Identity = 91/143 (63.64%), Postives = 113/143 (79.02%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCA------XXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLC 509
            +CP MKD+CTNP+VA +CKKTC  ST+   S+  SSISST ISTEPT+CP MKD+C+NP+++ +CKKTCA      SSTS SS+SST  STEPT+CP MKD+CTNP+V+ +CKKTC S+TT KSS+  +S SS P STEPTLC
Sbjct:  925 VCPDMKDMCTNPSVAKLCKKTCAFSTTPKSSSRGSSISSTQISTEPTVCPDMKDMCINPSIAKLCKKTCASSTKSKSSTSGSSVSSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCGSTTTGKSSTNVSSVSSTPKSTEPTLC 1067          

HSP 6 Score: 89.7373 bits (221), Expect = 4.399e-21
Identity = 72/116 (62.07%), Postives = 89/116 (76.72%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXX-----XXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CKKTC SST+S  ST  SSISST ISTEPT+CP MKD+C NP+V+ +CK TCASST+  S +          TEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  777 VCPDMKDMCTNPSVAKLCKKTCASSTTSKSSTSGSSISSTQISTEPTVCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTKGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCA 892          

HSP 7 Score: 87.8113 bits (216), Expect = 1.681e-20
Identity = 68/117 (58.12%), Postives = 89/117 (76.07%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX------EPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CKKTC SST+   ST  SS SST +STEPT+CP MK++C NP+V+ +CKKTCAS+T+  S +S    +      EPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  683 VCPDMKDMCTNPSVAKLCKKTCASSTTQESSTSGSSKSSTSMSTEPTVCPDMKEMCTNPSVAKLCKKTCASTTTPKSSTSGSSISSTPTSTEPTVCPDMKDMCTNPSVAKLCKKTCA 799          

HSP 8 Score: 87.4261 bits (215), Expect = 2.569e-20
Identity = 73/125 (58.40%), Postives = 93/125 (74.40%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAX--------------XXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            LCP +KDLC NP+V+ +CKKTCGSST++  S   SSI+S P+STEPT+CP MKD+C NP+V+ +CKKTCA               STS SS+SST +STEPT+CP MKD+CTNP+V+ +CKKTC 
Sbjct:  362 LCPKLKDLCLNPSVSGVCKKTCGSSTTAKSSVSASSITSKPVSTEPTVCPDMKDMCTNPSVAKLCKKTCAFPTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCG 486          

HSP 9 Score: 81.6481 bits (200), Expect = 2.403e-18
Identity = 70/125 (56.00%), Postives = 89/125 (71.20%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX--------------EPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CK TC SST+   ST  SSISSTPI+ EPT+CP MKD+C NP+V+ +CKKTCASST+  S +S    +              EPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  824 VCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTKGSSISSTPIT-EPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCA 947          

HSP 10 Score: 81.2629 bits (199), Expect = 3.271e-18
Identity = 71/132 (53.79%), Postives = 88/132 (66.67%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXX--------XXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXX-------------XEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CKKTCGSST+   ST  SS SST          STEPT+CP MKD+C NP+V+ +CK TCASST+  S +S                 TEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  464 VCPDMKDMCTNPSVAKLCKKTCGSSTTPRSSTSGSSKSSTSGSSISSKPMSTEPTVCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTSESSKSSTSGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCA 595          

HSP 11 Score: 79.7221 bits (195), Expect = 1.069e-17
Identity = 72/133 (54.14%), Postives = 90/133 (67.67%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXX--------XXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXX--------------EPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CKKTC SST+   ST  SS SS        TPISTEPT+CP MKD+C NP+V+ +CKKTCASST+  S +S    +              EPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  573 VCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGPSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGSSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCA 705          

HSP 12 Score: 78.9518 bits (193), Expect = 2.332e-17
Identity = 72/132 (54.55%), Postives = 90/132 (68.18%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXX-------XEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXX--------------XXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+CTNP+VA +CK TC SST+   ST  SS SST  S       TEPT+CP MKD+C NP+V+ +CKKTCASST+  S +S              T +STEPT+CP MKD+CTNP+V+ +CKKTCA
Sbjct:  519 VCPDMKDMCTNPSVAKLCKNTCASSTTPKSSTSESSKSSTSGSSISSTPITEPTVCPDMKDMCTNPSVAKLCKKTCASSTTPKSSTSGSSKSSTSGPSISSTPISTEPTVCPDMKDMCTNPSVAKLCKKTCA 650          

HSP 13 Score: 77.0258 bits (188), Expect = 9.323e-17
Identity = 63/116 (54.31%), Postives = 87/116 (75.00%), Query Frame = 3
Query:   99 LCPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX-----XXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            +CP MKD+C+   V+ +CKKTCGSSTS+  ST I++IS+TP+STEP++CP MKD+C  P +S++CKKTCA+S+   S S+       + TEPT CP MK+ C+NP V++MCKKTC 
Sbjct:  100 ICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLSTEPSVCPAMKDICNLPTISVMCKKTCATSSIGKSSSTFSISTTPIFTEPTQCPDMKNYCSNPGVALMCKKTCG 215          

HSP 14 Score: 75.485 bits (184), Expect = 2.929e-16
Identity = 80/142 (56.34%), Postives = 102/142 (71.83%), Query Frame = 3
Query:  102 CPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXX------XXXEPTICPAMKDLCTNPAVSVMCKKTCAXXXXXXXXXXXXXXXXXXXXXEPTLC 509
            CP MKD+C   +V+++CKKTC        ST +SS S TPISTEPT CP MKDLC+NP V+ +CKKTC S+T + S +S        +STEP++CP +KDLC NP+VS +CKKTC SSTT KSS +A+S +S P STEPT+C
Sbjct:  275 CPLMKDICHLQSVSIICKKTCA------SSTPMSSKSPTPISTEPTQCPNMKDLCINPTVASMCKKTCGSTTPSKSSTSGSTATSTPISTEPSLCPKLKDLCLNPSVSGVCKKTCGSSTTAKSSVSASSITSKPVSTEPTVC 410          

HSP 15 Score: 67.781 bits (164), Expect = 1.554e-13
Identity = 46/67 (68.66%), Postives = 51/67 (76.12%), Query Frame = 3
Query:  231 EPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            EPT CP MKD+C   +VS+ICKKTCASST  SS S T +STEPT CP MKDLC NP V+ MCKKTC 
Sbjct:  271 EPTQCPLMKDICHLQSVSIICKKTCASSTPMSSKSPTPISTEPTQCPNMKDLCINPTVASMCKKTCG 337          

HSP 16 Score: 67.0106 bits (162), Expect = 2.789e-13
Identity = 55/116 (47.41%), Postives = 70/116 (60.34%), Query Frame = 3
Query:  102 CPAMKDLCTNPAVAVMCKKTCGXXXXXXXXXXXXXXXXXXXXXEPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXX------XXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            C  M  +C NP V+ +C KTC S+T ++  +            EPT+CP MKD+C    VS +CKKTC SSTS  S +S         STEP++CPAMKD+C  P +SVMCKKTCA
Sbjct:   64 CAFMTQICHNPGVSQICPKTCASNTFTSTESPT----------EPTICPLMKDICSLETVSSLCKKTCGSSTSTKSSTSITTISTTPLSTEPSVCPAMKDICNLPTISVMCKKTCA 169          

HSP 17 Score: 53.5286 bits (127), Expect = 1.479e-8
Identity = 35/67 (52.24%), Postives = 43/67 (64.18%), Query Frame = 3
Query:  231 EPTLCPQMKDLCMNPAVSLICKKTCAXXXXXXXXXXXXXXXEPTICPAMKDLCTNPAVSVMCKKTCA 431
            EP  C  M  +C NP VS IC KTCA    +++ +ST   TEPTICP MKD+C+   VS +CKKTC 
Sbjct:   60 EPAQCAFMTQICHNPGVSQICPKTCA----SNTFTSTESPTEPTICPLMKDICSLETVSSLCKKTCG 122          
The following BLAST results are available for this feature:
BLAST of SMED30024100 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024100 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024100 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024100 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024100 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024100 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024100 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024100 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024100 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024100 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024100 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024100 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024100 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30024100 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000066354.16.327e-3499.27SMESG000066354.1[more]
SMESG000066354.16.327e-3499.27SMESG000066354.1[more]
SMESG000066354.17.049e-3499.27SMESG000066354.1[more]
SMESG000066354.17.117e-3499.27SMESG000066354.1[more]
SMESG000066354.17.176e-3499.27SMESG000066354.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024100 ID=SMED30024100|Name=SMED30024100|organism=Schmidtea mediterranea sexual|type=transcript|length=509bp
CCAAATTGTGTAAAAAGACATGTGGTTCTACTACCACTGGAAAATCTTCA
ACCAGTGTATCATCGGTTTCATCAACACCTAAAAGCACCGAGCCAACATT
ATGCCCAGCAATGAAAGATTTGTGTACAAATCCCGCTGTCGCAGTTATGT
GCAAGAAAACATGCGGCTCATCCACCTCCTCGACATTGTCCACATTCATT
TCATCTATATCATCAACACCTATAAGTACTGAGCCTACATTATGCCCGCA
AATGAAAGATTTATGCATGAATCCAGCAGTGTCACTGATATGCAAAAAGA
CATGCGCTTCATCAACAAGTGCTTCATCTTTGTCCTCTACAATTATGAGT
ACTGAACCAACAATATGTCCTGCAATGAAGGATCTATGCACAAATCCAGC
TGTGTCAGTTATGTGCAAAAAAACATGTGCCTCATCAACAACACTAAAAT
CATCCTCAACTGCCACTTCTCAGTCTTCTGTGCCAACTAGCACTGAACCT
ACTTTATGT
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protein sequence of SMED30024100-orf-1

>SMED30024100-orf-1 ID=SMED30024100-orf-1|Name=SMED30024100-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=169bp
KLCKKTCGSTTTGKSSTSVSSVSSTPKSTEPTLCPAMKDLCTNPAVAVMC
KKTCGSSTSSTLSTFISSISSTPISTEPTLCPQMKDLCMNPAVSLICKKT
CASSTSASSLSSTIMSTEPTICPAMKDLCTNPAVSVMCKKTCASSTTLKS
SSTATSQSSVPTSTEPTLC
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR003582ShKT_dom
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003582ShKT domainPFAMPF01549ShKcoord: 121..142
e-value: 0.26
score: 11.9
coord: 33..55
e-value: 0.028
score: 15.0
coord: 80..101
e-value: 1.5
score: 9.4