Tetraspanin

Overview
NameTetraspanin
Smed IDSMED30024093
Length (bp)913
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Tetraspanin (SMED30024093) t-SNE clustered cells

Violin plots show distribution of expression levels for Tetraspanin (SMED30024093) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Tetraspanin (SMED30024093) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Tetraspanin (SMED30024093) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30024093

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30024093h1SMcG0007774 Contig2237GPL15192PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
Smed sexual biotypeSMED30024093h1SMcG0007774 Contig51744newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30024093h1SMcG0007774 Contig51744uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
protonephridiaSMED30024093h1SMcG0007774 dd_Smed_v4_440_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30024093h1SMcG0007774 dd_Smed_v4_440_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30024093h1SMcG0007774 dd_Smed_v4_440_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30024093h1SMcG0007774 dd_Smed_v4_440_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30024093h1SMcG0007774 dd_Smed_v4_440_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30024093h1SMcG0007774 dd_Smed_v4_440_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30024093h1SMcG0007774 dd_Smed_v6_440_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30024093h1SMcG0007774 dd_Smed_v6_440_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Tetraspanin vs. Ensembl Human
Match: CD63 (CD63 molecule [Source:HGNC Symbol;Acc:HGNC:1692])

HSP 1 Score: 69.3218 bits (168), Expect = 3.655e-13
Identity = 56/234 (23.93%), Postives = 108/234 (46.15%), Query Frame = 1
Query:   55 IMSGLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKY--FADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDY----IFNNAEIPKSCCGNIK-----NCTELEAFKKGCEKPTEAFVSYVLTTMAXXXXXXXXXXXXXXXFTFCFVKSMNN 723
            +  G+   KF    + L F    + L  +    Q  L       A    + P V  ++A+G+ +  ++ +G    C E  CL+  F + L LI+L++ +AA+  +V+++ ++ EF+N      ++    N  +    D++Q  +KCCG  N  D+      +   +P SCC N+      N  E    K+GC +    ++   +  +A   + +  ++V+ I+F  C VKS+ +
Sbjct:    3 VEGGMKCVKFLLYVLLLAFCACAVGLIAVGVGAQLVLSQTIIQGATPGSLLPVV--IIAVGVFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAAAIAGYVFRDKVMSEFNNNFRQQMENYPKNNH-TASILDRMQADFKCCGAANYTDWEKIPSMSKNRVPDSCCINVTVGCGINFNEKAIHKEGCVEKIGGWLRKNVLVVAAAALGIAFVEVLGIVFACCLVKSIRS 233          
BLAST of Tetraspanin vs. Ensembl Human
Match: CD63 (CD63 molecule [Source:HGNC Symbol;Acc:HGNC:1692])

HSP 1 Score: 69.3218 bits (168), Expect = 3.655e-13
Identity = 56/234 (23.93%), Postives = 108/234 (46.15%), Query Frame = 1
Query:   55 IMSGLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKY--FADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDY----IFNNAEIPKSCCGNIK-----NCTELEAFKKGCEKPTEAFVSYVLTTMAXXXXXXXXXXXXXXXFTFCFVKSMNN 723
            +  G+   KF    + L F    + L  +    Q  L       A    + P V  ++A+G+ +  ++ +G    C E  CL+  F + L LI+L++ +AA+  +V+++ ++ EF+N      ++    N  +    D++Q  +KCCG  N  D+      +   +P SCC N+      N  E    K+GC +    ++   +  +A   + +  ++V+ I+F  C VKS+ +
Sbjct:    3 VEGGMKCVKFLLYVLLLAFCACAVGLIAVGVGAQLVLSQTIIQGATPGSLLPVV--IIAVGVFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAAAIAGYVFRDKVMSEFNNNFRQQMENYPKNNH-TASILDRMQADFKCCGAANYTDWEKIPSMSKNRVPDSCCINVTVGCGINFNEKAIHKEGCVEKIGGWLRKNVLVVAAAALGIAFVEVLGIVFACCLVKSIRS 233          
BLAST of Tetraspanin vs. Ensembl Human
Match: CD63 (CD63 molecule [Source:HGNC Symbol;Acc:HGNC:1692])

HSP 1 Score: 69.3218 bits (168), Expect = 3.655e-13
Identity = 56/234 (23.93%), Postives = 108/234 (46.15%), Query Frame = 1
Query:   55 IMSGLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKY--FADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDY----IFNNAEIPKSCCGNIK-----NCTELEAFKKGCEKPTEAFVSYVLTTMAXXXXXXXXXXXXXXXFTFCFVKSMNN 723
            +  G+   KF    + L F    + L  +    Q  L       A    + P V  ++A+G+ +  ++ +G    C E  CL+  F + L LI+L++ +AA+  +V+++ ++ EF+N      ++    N  +    D++Q  +KCCG  N  D+      +   +P SCC N+      N  E    K+GC +    ++   +  +A   + +  ++V+ I+F  C VKS+ +
Sbjct:    3 VEGGMKCVKFLLYVLLLAFCACAVGLIAVGVGAQLVLSQTIIQGATPGSLLPVV--IIAVGVFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAAAIAGYVFRDKVMSEFNNNFRQQMENYPKNNH-TASILDRMQADFKCCGAANYTDWEKIPSMSKNRVPDSCCINVTVGCGINFNEKAIHKEGCVEKIGGWLRKNVLVVAAAALGIAFVEVLGIVFACCLVKSIRS 233          
BLAST of Tetraspanin vs. Ensembl Human
Match: CD63 (CD63 molecule [Source:HGNC Symbol;Acc:HGNC:1692])

HSP 1 Score: 69.3218 bits (168), Expect = 3.655e-13
Identity = 56/234 (23.93%), Postives = 108/234 (46.15%), Query Frame = 1
Query:   55 IMSGLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKY--FADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDY----IFNNAEIPKSCCGNIK-----NCTELEAFKKGCEKPTEAFVSYVLTTMAXXXXXXXXXXXXXXXFTFCFVKSMNN 723
            +  G+   KF    + L F    + L  +    Q  L       A    + P V  ++A+G+ +  ++ +G    C E  CL+  F + L LI+L++ +AA+  +V+++ ++ EF+N      ++    N  +    D++Q  +KCCG  N  D+      +   +P SCC N+      N  E    K+GC +    ++   +  +A   + +  ++V+ I+F  C VKS+ +
Sbjct:    3 VEGGMKCVKFLLYVLLLAFCACAVGLIAVGVGAQLVLSQTIIQGATPGSLLPVV--IIAVGVFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAAAIAGYVFRDKVMSEFNNNFRQQMENYPKNNH-TASILDRMQADFKCCGAANYTDWEKIPSMSKNRVPDSCCINVTVGCGINFNEKAIHKEGCVEKIGGWLRKNVLVVAAAALGIAFVEVLGIVFACCLVKSIRS 233          
BLAST of Tetraspanin vs. Ensembl Human
Match: CD63 (CD63 molecule [Source:HGNC Symbol;Acc:HGNC:1692])

HSP 1 Score: 68.5514 bits (166), Expect = 4.422e-13
Identity = 49/193 (25.39%), Postives = 96/193 (49.74%), Query Frame = 1
Query:  172 FADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDY----IFNNAEIPKSCCGNIK-----NCTELEAFKKGCEKPTEAFVSYVLTTMAXXXXXXXXXXXXXXXFTFCFVKSMNN 723
            F  A    + P V  ++A+G+ +  ++ +G    C E  CL+  F + L LI+L++ +AA+  +V+++ ++ EF+N      ++    N  +    D++Q  +KCCG  N  D+      +   +P SCC N+      N  E    K+GC +    ++   +  +A   + +  ++V+ I+F  C VKS+ +
Sbjct:   21 FCGATPGSLLPVV--IIAVGVFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAAAIAGYVFRDKVMSEFNNNFRQQMENYPKNNH-TASILDRMQADFKCCGAANYTDWEKIPSMSKNRVPDSCCINVTVGCGINFNEKAIHKEGCVEKIGGWLRKNVLVVAAAALGIAFVEVLGIVFACCLVKSIRS 210          
BLAST of Tetraspanin vs. Ensembl Celegans
Match: tsp-7 (Tetraspanin [Source:UniProtKB/TrEMBL;Acc:Q9XVI4])

HSP 1 Score: 68.1662 bits (165), Expect = 2.995e-13
Identity = 47/192 (24.48%), Postives = 86/192 (44.79%), Query Frame = 1
Query:   55 IMSGLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYIFNNAEIPKSCC-GNIKNCTELEA--FKKGCEKPTEAFV 621
            +  G+   K+     NL+  + G++L ++ ++ Q       D L      +   +L IG +   +  LG      E  CL   F VLL L++  + +A +  +   ++      N + T      + ++G +  +DK  Q ++CCG+ N++D++     IP SCC   I+ C    A  F+ GC    E +V
Sbjct:    2 VEGGVTIVKYLLFLANLVLWVGGLSLIIVGSILQLKFDNVLDILGDERLATPILLLVIGSLCTLLGFLGCCGAIRENYCLTVSFAVLLALLITCEIAAVIIGYALHDSFRLGIGNQLQTGMVR-YHESRGVESAWDKTHQLFECCGVTNSSDWL-TFTTIPDSCCIEEIEGCARENAPLFEPGCIHSVEQWV 191          
BLAST of Tetraspanin vs. Ensembl Celegans
Match: tsp-8 (Tetraspanin [Source:UniProtKB/TrEMBL;Acc:Q19983])

HSP 1 Score: 56.225 bits (134), Expect = 6.475e-9
Identity = 43/149 (28.86%), Postives = 71/149 (47.65%), Query Frame = 1
Query:   85 TFVFINLIFLLSGIT---LTVIAALGQSNLKYFADALNIP-IFPSV-TFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYI 516
            TF+F N  F LSG+    L +      +   +FA     P  F  V  F++  G I+I +   G         C L  F  +LIL   ++ +AAVT F  + +I    +++M  T ++  +     KD FD +Q+K++CCG+    D++
Sbjct:   12 TFLF-NFAFWLSGVVVFGLGIWLLFDPAASDFFALHSTHPGAFRYVGWFLVGAGAIIILVGYFGCIGAWKMNQCALAFFCCILILAFFLELAAAVTLFHKQEHIKHYVESSMYDTIRNRYSSETAFKDAFDTVQEKFECCGVKTYTDWL 159          
BLAST of Tetraspanin vs. Ensembl Celegans
Match: tsp-6 (Tetraspanin [Source:UniProtKB/TrEMBL;Acc:G4RWX0])

HSP 1 Score: 53.1434 bits (126), Expect = 5.433e-8
Identity = 44/179 (24.58%), Postives = 78/179 (43.58%), Query Frame = 1
Query:   76 AKFTFVFINLIFLLSGI--------------TLTVIAALGQSNLKYFADALNIPIFPSVTFV-LAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYIFNNAEIPKSC-CGNIKN 564
             K+ F  IN +F + G               +L  +A+  + +L      +NI    S  +V + IG  ++ +   G    C E  C ++++ +L++++ +++  A V  FV K N+ + F         S  N  +      D+IQ   +CCG +  +DYI   A  P SC C  I+ 
Sbjct:   10 VKYFFWLINFLFFVLGAIIVGLSIWMLVDKNSLNTVASTVKVDLSQILSQVNIQQLNSFLYVAIVIGGALLVLGFFGCCGSCCESICAISIYFILVLILFVVEVVAIVLYFVNKTNLQQGFQTIWRDELVSKYNTQQQIHQVLDQIQSSLQCCGASGCSDYIPYGA-FPTSCQCATIQQ 187          
BLAST of Tetraspanin vs. Ensembl Fly
Match: Tsp39D (gene:FBgn0032943 transcript:FBtr0273404)

HSP 1 Score: 75.0998 bits (183), Expect = 1.255e-15
Identity = 47/188 (25.00%), Postives = 90/188 (47.87%), Query Frame = 1
Query:   64 GLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDK---IQQKYKCCGLNNANDY--IFNNAEIPKSCCGNI-----KNCTELEAFKKGC 597
            GL   K+   F NL+F L+G+ + ++  + Q N  ++++ ++  ++ +   ++ +G  V  I  LG      E +C++  F +L ++I L +    +  +V    +       M + F S +   K   D+ D    +Q +  CCG+N  ND+  ++ N+ +P +CC  I     K CT   A + GC
Sbjct:    5 GLTCVKYLTFFCNLLFALTGLLIFLVGGMVQLNYAHYSNFVSDHVWTAPIILMIVGAAVAVICFLGCCGALKESSCMILSFALLAVVIFLFEIGLGLAGYVKHTGL----HQIMESQFNSTMQHYKERADYRDAWTLLQTELDCCGINGPNDWETVYRNSTLPAACCSVINLSEAKECTNTHATQHGC 188          
BLAST of Tetraspanin vs. Ensembl Fly
Match: Tsp42Ea (gene:FBgn0029508 transcript:FBtr0086171)

HSP 1 Score: 66.2402 bits (160), Expect = 1.659e-12
Identity = 52/182 (28.57%), Postives = 86/182 (47.25%), Query Frame = 1
Query:   64 GLFAAKFTFVFINLIFLLSGITLTVIAALGQS---NLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYIFNNAEIPKSCCGNIKNCTELEAFKKGCE 600
            G+   K+     NL+  + GI L V  AL  S   N+  FA+AL     P VT ++ +G I++ IS  G      E  C+   + +LL ++++ Q +  +  +V K+    ++   M    + A N      D+ D IQ   KCCG +   DY +   + P SCC +  NC     +++GC+
Sbjct:    4 GISMVKYILFIFNLLCSICGILLIVFGALLFSKVRNMDDFAEALRTQQVP-VT-MIILGTIILLISWFGCCGAIRESYCMSMTYSILLFVLMIGQLALVIYMWVQKD----KYLEIMGDVVEKAWNHRTSRSDYMDAIQISMKCCGRSGYTDYAY-QGKFPPSCCSDTNNCRWETVYRRGCK 178          
BLAST of Tetraspanin vs. Ensembl Fly
Match: Tsp42Ea (gene:FBgn0029508 transcript:FBtr0086173)

HSP 1 Score: 66.2402 bits (160), Expect = 1.659e-12
Identity = 52/182 (28.57%), Postives = 86/182 (47.25%), Query Frame = 1
Query:   64 GLFAAKFTFVFINLIFLLSGITLTVIAALGQS---NLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYIFNNAEIPKSCCGNIKNCTELEAFKKGCE 600
            G+   K+     NL+  + GI L V  AL  S   N+  FA+AL     P VT ++ +G I++ IS  G      E  C+   + +LL ++++ Q +  +  +V K+    ++   M    + A N      D+ D IQ   KCCG +   DY +   + P SCC +  NC     +++GC+
Sbjct:    4 GISMVKYILFIFNLLCSICGILLIVFGALLFSKVRNMDDFAEALRTQQVP-VT-MIILGTIILLISWFGCCGAIRESYCMSMTYSILLFVLMIGQLALVIYMWVQKD----KYLEIMGDVVEKAWNHRTSRSDYMDAIQISMKCCGRSGYTDYAY-QGKFPPSCCSDTNNCRWETVYRRGCK 178          
BLAST of Tetraspanin vs. Ensembl Fly
Match: Tsp42Ea (gene:FBgn0029508 transcript:FBtr0086172)

HSP 1 Score: 66.2402 bits (160), Expect = 1.659e-12
Identity = 52/182 (28.57%), Postives = 86/182 (47.25%), Query Frame = 1
Query:   64 GLFAAKFTFVFINLIFLLSGITLTVIAALGQS---NLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYIFNNAEIPKSCCGNIKNCTELEAFKKGCE 600
            G+   K+     NL+  + GI L V  AL  S   N+  FA+AL     P VT ++ +G I++ IS  G      E  C+   + +LL ++++ Q +  +  +V K+    ++   M    + A N      D+ D IQ   KCCG +   DY +   + P SCC +  NC     +++GC+
Sbjct:    4 GISMVKYILFIFNLLCSICGILLIVFGALLFSKVRNMDDFAEALRTQQVP-VT-MIILGTIILLISWFGCCGAIRESYCMSMTYSILLFVLMIGQLALVIYMWVQKD----KYLEIMGDVVEKAWNHRTSRSDYMDAIQISMKCCGRSGYTDYAY-QGKFPPSCCSDTNNCRWETVYRRGCK 178          
BLAST of Tetraspanin vs. Ensembl Fly
Match: Tsp29Fb (gene:FBgn0032075 transcript:FBtr0079756)

HSP 1 Score: 67.0106 bits (162), Expect = 2.468e-12
Identity = 48/198 (24.24%), Postives = 94/198 (47.47%), Query Frame = 1
Query:   61 SGLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYI------------FNNAEIPKSCCGN----IKNCTE---LEAFKKGC 597
            SG+  AK+  + ++ +F L+ I L ++    Q+    F+  ++         ++AIG I+IA++ LG +    E   ++ ++GV L L+ +++ SAA+  FV ++ +      TMN    +    +   +   D +Q   +CCG+N   D+              ++  +P SCCGN    + + T+   +E +  GC
Sbjct:   10 SGMKCAKYMLIIVSFMFALTAILLIMVGTTIQTIFGDFSLFIDGHFSSPPALLIAIGFILIAVAALGAYGAVKESVMVINLYGVCLFLVFILEVSAAIAAFVMQSQVRGMLIRTMNQAL-AEYEHDPYVESGVDFMQSMLECCGVNEPEDWKDYLSANVNFTLGVDDVVVPNSCCGNQPTSLNDSTQMTCMETYDYGC 206          
BLAST of Tetraspanin vs. Ensembl Zebrafish
Match: cd63 (CD63 molecule [Source:ZFIN;Acc:ZDB-GENE-030131-180])

HSP 1 Score: 75.485 bits (184), Expect = 1.604e-15
Identity = 48/167 (28.74%), Postives = 84/167 (50.30%), Query Frame = 1
Query:   79 KFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYI---FNNAEIPKSCCGNI-KNC 567
            K+   F N IF L G+ L V+  L   +L   A        P V  V  +G+I+  IS  G      E  C++  F ++L LI++ +  A +  ++++  + +  D + NT   +  N  +  +   D IQ++ KCCG N+++D++    ++  +P SCC N+ KNC
Sbjct:   11 KYLLFFFNFIFWLCGLALIVLGILVHVSLHNTAILQGASGSPMVLIV--VGVIIFFISFFGCCGAWKENQCMVVTFAIILSLIVITEIGAGIAGYIFRGKVNELLDQSFNTMI-AGYNKTEEYRTTLDSIQKQLKCCGGNSSSDWVNFSADHISVPDSCCKNVTKNC 174          
BLAST of Tetraspanin vs. Ensembl Zebrafish
Match: cd63 (CD63 molecule [Source:ZFIN;Acc:ZDB-GENE-030131-180])

HSP 1 Score: 75.485 bits (184), Expect = 1.604e-15
Identity = 48/167 (28.74%), Postives = 84/167 (50.30%), Query Frame = 1
Query:   79 KFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYI---FNNAEIPKSCCGNI-KNC 567
            K+   F N IF L G+ L V+  L   +L   A        P V  V  +G+I+  IS  G      E  C++  F ++L LI++ +  A +  ++++  + +  D + NT   +  N  +  +   D IQ++ KCCG N+++D++    ++  +P SCC N+ KNC
Sbjct:   11 KYLLFFFNFIFWLCGLALIVLGILVHVSLHNTAILQGASGSPMVLIV--VGVIIFFISFFGCCGAWKENQCMVVTFAIILSLIVITEIGAGIAGYIFRGKVNELLDQSFNTMI-AGYNKTEEYRTTLDSIQKQLKCCGGNSSSDWVNFSADHISVPDSCCKNVTKNC 174          
BLAST of Tetraspanin vs. Ensembl Zebrafish
Match: zgc:64051 (zgc:64051 [Source:ZFIN;Acc:ZDB-GENE-040426-1349])

HSP 1 Score: 63.1586 bits (152), Expect = 2.252e-11
Identity = 58/205 (28.29%), Postives = 95/205 (46.34%), Query Frame = 1
Query:   58 MSGLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTF------VLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVY----KNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYIFNNAEIPKSCCGNIKNCTELEAFKKGCEKPTE-AFVSYVLTT 639
            MS L   K+    +N IF + G     I  +G     Y      + + PS+        +   GII+  +S LG      E  CLL  F +LL +++L + +AA    +Y    +N I  +  + +N + K+    N  + D +DK+Q+ + CCG+ NA D+      +P+SC     N +    + KGC K  E +F S +L+T
Sbjct:    1 MSCLKCLKYIMCVVNFIFFICG---AAIFGMG----IYLMTFSRLSLLPSLQAMSIANTLFITGIIITCVSFLGFLGALKENRCLLISFFILLFILMLAELAAACLMLMYESKIENFIKDDLVDGLNQSIKNRKQHN--TTDDWDKVQETFGCCGIQNATDW---QGFVPQSC-----NISGTSNWHKGCFKLLENSFESNLLST 188          
BLAST of Tetraspanin vs. Ensembl Zebrafish
Match: tspan7 (tetraspanin 7 [Source:ZFIN;Acc:ZDB-GENE-030131-5435])

HSP 1 Score: 63.1586 bits (152), Expect = 3.623e-11
Identity = 53/175 (30.29%), Postives = 86/175 (49.14%), Query Frame = 1
Query:  100 NLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVL-AIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDF-FDKIQQKYKCCGLNN----ANDYIFNNAEIPKSCCGNIK---NCT--ELEAFKK 591
            +LIF  +G+ L  +   G+ NL+ F   +N     +  +VL   G ++I   L G FA C     +L ++ + L+L+ L +  A ++ F++++    E    +   ++ A +   G  D   D+IQ+  +CCG  N    AN   F    IPKSCC N+    NCT  EL+  KK
Sbjct:   25 SLIFWFTGVILLAVGVWGKVNLE-FDLLVNSEHGTNAPYVLIGTGAVIIIFGLFGCFATCRGSPWMLKLYAMFLVLVFLAELVAGISGFIFRH----EIKAVLKGAYQKAESNYMGKDDEDLDRIQRTLQCCGEENYTSWANTTYFEKEGIPKSCC-NVTASVNCTSAELKDLKK 193          
BLAST of Tetraspanin vs. Ensembl Zebrafish
Match: cd151l (CD151 antigen, like [Source:ZFIN;Acc:ZDB-GENE-040426-1868])

HSP 1 Score: 61.6178 bits (148), Expect = 1.127e-10
Identity = 56/240 (23.33%), Postives = 108/240 (45.00%), Query Frame = 1
Query:   64 GLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAI-GIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSK--DFFDKIQQKYKCCGLNNANDYI----FNNAE-----IPKSC--------CGNIKNCTELEAFKKGCEKPTEAFVSYVLTTMAXXXXXXXXXXXXXXXFTFCFVKSMNN 723
            G    K+     N +F L+G+ +  +          +   L+  I+    ++L + G+IV+   +LG  A   E+  LL V+ VLL+ I L++  A V  ++Y   +  E    +  T     N ++        DK+QQ++KCCG N+++D++      ++E     +P SC        CG   + + +   + GC    E F+   L  +  V + +  + ++ + FT C  +S+ +
Sbjct:   12 GTICLKYLLFTFNFLFWLAGVAVMAVGIWTVIEKSDYISLLSSKIYAVSAYILIMAGVIVMITGVLGCCATFKEQRRLLRVYFVLLLCIFLLEILAGVLAYIYYQQLNDELKENLRETMVQKYNQSEQEHVTKAVDKLQQEFKCCGSNSSSDWVDSAWIRSSEADGRLVPDSCCKSPVRKFCGRRDHPSNIYKVEGGCITKLENFILNHLQIIGAVGVGVASVQIVGMFFTCCLYRSLKS 251          
BLAST of Tetraspanin vs. Ensembl Xenopus
Match: asmt (acetylserotonin O-methyltransferase [Source:Xenbase;Acc:XB-GENE-942483])

HSP 1 Score: 69.3218 bits (168), Expect = 3.603e-13
Identity = 45/167 (26.95%), Postives = 78/167 (46.71%), Query Frame = 1
Query:   67 LFAAKFTFVFINLIFLLSGI-TLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALN-----GNKGSKDFFDKIQQKYKCCGLNNANDYIFNNAEIPKSCC 549
            L   K T    NLIF L G   L V   L  +  K+   +++ P   + +  +  G I++ +  +G     +E  CLL  F V+L++I L++  +      Y     ++F N      K  L      GN G  + +D +Q +++CCG+ NA D++ +   +P +CC
Sbjct:    8 LLCIKITMFIFNLIFWLGGCGILGVGVWLAVTQGKFATLSVSFPSLSAASLFMVTGSIIMVVGFIGCLGAVTEHRCLLLTFFVILLIIFLLEMISMALFLTYT----EQFHNYAQEDLKKGLQLYNTTGNLGLTNAWDIVQTEFRCCGVKNATDWLESKGSVPHTCC 170          
BLAST of Tetraspanin vs. Ensembl Xenopus
Match: ENSXETT00000022335.1 (tetraspanin-4-like [Source:NCBI gene;Acc:101734690])

HSP 1 Score: 68.9366 bits (167), Expect = 3.789e-13
Identity = 45/167 (26.95%), Postives = 78/167 (46.71%), Query Frame = 1
Query:   67 LFAAKFTFVFINLIFLLSGI-TLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALN-----GNKGSKDFFDKIQQKYKCCGLNNANDYIFNNAEIPKSCC 549
            L   K T    NLIF L G   L V   L  +  K+   +++ P   + +  +  G I++ +  +G     +E  CLL  F V+L++I L++  +      Y     ++F N      K  L      GN G  + +D +Q +++CCG+ NA D++ +   +P +CC
Sbjct:    6 LLCIKITMFIFNLIFWLGGCGILGVGVWLAVTQGKFATLSVSFPSLSAASLFMVTGSIIMVVGFIGCLGAVTEHRCLLLTFFVILLIIFLLEMISMALFLTYT----EQFHNYAQEDLKKGLQLYNTTGNLGLTNAWDIVQTEFRCCGVKNATDWLESKGSVPHTCC 168          
BLAST of Tetraspanin vs. Ensembl Xenopus
Match: asmt (acetylserotonin O-methyltransferase [Source:Xenbase;Acc:XB-GENE-942483])

HSP 1 Score: 68.9366 bits (167), Expect = 4.971e-13
Identity = 45/167 (26.95%), Postives = 78/167 (46.71%), Query Frame = 1
Query:   67 LFAAKFTFVFINLIFLLSGI-TLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALN-----GNKGSKDFFDKIQQKYKCCGLNNANDYIFNNAEIPKSCC 549
            L   K T    NLIF L G   L V   L  +  K+   +++ P   + +  +  G I++ +  +G     +E  CLL  F V+L++I L++  +      Y     ++F N      K  L      GN G  + +D +Q +++CCG+ NA D++ +   +P +CC
Sbjct:   27 LLCIKITMFIFNLIFWLGGCGILGVGVWLAVTQGKFATLSVSFPSLSAASLFMVTGSIIMVVGFIGCLGAVTEHRCLLLTFFVILLIIFLLEMISMALFLTYT----EQFHNYAQEDLKKGLQLYNTTGNLGLTNAWDIVQTEFRCCGVKNATDWLESKGSVPHTCC 189          
BLAST of Tetraspanin vs. Ensembl Xenopus
Match: ENSXETT00000022348.1 (tetraspanin-4-like [Source:NCBI gene;Acc:101734690])

HSP 1 Score: 68.5514 bits (166), Expect = 1.593e-12
Identity = 45/167 (26.95%), Postives = 78/167 (46.71%), Query Frame = 1
Query:   67 LFAAKFTFVFINLIFLLSGI-TLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALN-----GNKGSKDFFDKIQQKYKCCGLNNANDYIFNNAEIPKSCC 549
            L   K T    NLIF L G   L V   L  +  K+   +++ P   + +  +  G I++ +  +G     +E  CLL  F V+L++I L++  +      Y     ++F N      K  L      GN G  + +D +Q +++CCG+ NA D++ +   +P +CC
Sbjct:   86 LLCIKITMFIFNLIFWLGGCGILGVGVWLAVTQGKFATLSVSFPSLSAASLFMVTGSIIMVVGFIGCLGAVTEHRCLLLTFFVILLIIFLLEMISMALFLTY----TEQFHNYAQEDLKKGLQLYNTTGNLGLTNAWDIVQTEFRCCGVKNATDWLESKGSVPHTCC 248          
BLAST of Tetraspanin vs. Ensembl Xenopus
Match: sertad4 (SERTA domain containing 4 [Source:Xenbase;Acc:XB-GENE-943347])

HSP 1 Score: 67.3958 bits (163), Expect = 1.834e-12
Identity = 48/192 (25.00%), Postives = 86/192 (44.79%), Query Frame = 1
Query:   79 KFTFVFINLIFLLSGITLTVIAALGQSNLK-YFA----DALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDY----IFNNAEIPKSCCGNIKNCTELE----------AFKKGC 597
            K   +    IF ++G+ L  +   G+ +L  YF+    +A N+P       ++  G ++I +   G FA C     +L ++ + L +I L++  AA+   V+++ I   F+       K             D IQ+  +CCG+N+ N++     +    IP+SCC    NC+E +           FK+GC
Sbjct:   18 KSVLLIYTFIFWITGVILLTVGVWGKVSLDIYFSLLNENATNVPYV-----LIGTGAVIILLGTFGCFATCRASTWMLKLYAMFLSIIFLVELIAAIVGLVFRHEIKNSFEQGYLQALKQYNATGDPRSQAVDTIQRTLQCCGVNSYNEWGQTQYYKTHGIPQSCCRVPGNCSEADMKDLGKAGGKVFKQGC 204          
BLAST of Tetraspanin vs. Ensembl Mouse
Match: Tspan6 (tetraspanin 6 [Source:MGI Symbol;Acc:MGI:1926264])

HSP 1 Score: 64.6994 bits (156), Expect = 9.663e-12
Identity = 47/166 (28.31%), Postives = 81/166 (48.80%), Query Frame = 1
Query:   79 KFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVL-AIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDF----FDKIQQKYKCCGLNNANDY----IFNNAEIPKSCC 549
            K   +    IF ++G+ L  +   G+ +L+ +   LN     +V FVL   G ++I +   G FA C     +L ++ + L LI L++  AA+  FV+++    E  N+  + +++AL     + D+     DKIQ    CCG+ N  D+     ++    PKSCC
Sbjct:   18 KSVLLIYTFIFWITGVILLAVGIWGKVSLENYFSLLNEKA-TNVPFVLIGTGTVIILLGTFGCFATCRASAWMLKLYAMFLTLIFLVELVAAIVGFVFRH----EIKNSFKSNYENALKEYNSTGDYRSEAVDKIQSTLHCCGVTNYGDWKGTNYYSETGFPKSCC 178          
BLAST of Tetraspanin vs. Ensembl Mouse
Match: Cd63 (CD63 antigen [Source:MGI Symbol;Acc:MGI:99529])

HSP 1 Score: 63.5438 bits (153), Expect = 2.153e-11
Identity = 58/234 (24.79%), Postives = 108/234 (46.15%), Query Frame = 1
Query:   55 IMSGLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKYF--ADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYI----FNNAEIPKSCCGNIK-NC----TELEAFKKGCEKPTEAFVSYVLTTMAXXXXXXXXXXXXXXXFTFCFVKSMNN 723
            +  G+   KF    + L F    + L  I    Q  LK     +     + P V  ++A+G  +  ++ +G    C E  CL+  F + L LI+L++ + A+  +V+++ +  EF+ +     ++ L  NK +    DK+Q++  CCG +N  D+          +P SCC NI   C     E     +GC +    ++   +  +A   + +  ++V+ IIF+ C VKS+ +
Sbjct:    3 VEGGMKCVKFLLYVLLLAFCACAVGLIAIGVAVQVVLKQAITHETTAGSLLPVV--IIAVGAFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAVAIAGYVFRDQVKSEFNKSFQQQMQNYLKDNK-TATILDKLQKENNCCGASNYTDWENIPGMAKDRVPDSCCINITVGCGNDFKESTIHTQGCVETIAIWLRKNILLVAAAALGIAFVEVLGIIFSCCLVKSIRS 233          
BLAST of Tetraspanin vs. Ensembl Mouse
Match: Cd63 (CD63 antigen [Source:MGI Symbol;Acc:MGI:99529])

HSP 1 Score: 63.5438 bits (153), Expect = 2.153e-11
Identity = 58/234 (24.79%), Postives = 108/234 (46.15%), Query Frame = 1
Query:   55 IMSGLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKYF--ADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYI----FNNAEIPKSCCGNIK-NC----TELEAFKKGCEKPTEAFVSYVLTTMAXXXXXXXXXXXXXXXFTFCFVKSMNN 723
            +  G+   KF    + L F    + L  I    Q  LK     +     + P V  ++A+G  +  ++ +G    C E  CL+  F + L LI+L++ + A+  +V+++ +  EF+ +     ++ L  NK +    DK+Q++  CCG +N  D+          +P SCC NI   C     E     +GC +    ++   +  +A   + +  ++V+ IIF+ C VKS+ +
Sbjct:    3 VEGGMKCVKFLLYVLLLAFCACAVGLIAIGVAVQVVLKQAITHETTAGSLLPVV--IIAVGAFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAVAIAGYVFRDQVKSEFNKSFQQQMQNYLKDNK-TATILDKLQKENNCCGASNYTDWENIPGMAKDRVPDSCCINITVGCGNDFKESTIHTQGCVETIAIWLRKNILLVAAAALGIAFVEVLGIIFSCCLVKSIRS 233          
BLAST of Tetraspanin vs. Ensembl Mouse
Match: Cd63 (CD63 antigen [Source:MGI Symbol;Acc:MGI:99529])

HSP 1 Score: 63.5438 bits (153), Expect = 2.153e-11
Identity = 58/234 (24.79%), Postives = 108/234 (46.15%), Query Frame = 1
Query:   55 IMSGLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKYF--ADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYI----FNNAEIPKSCCGNIK-NC----TELEAFKKGCEKPTEAFVSYVLTTMAXXXXXXXXXXXXXXXFTFCFVKSMNN 723
            +  G+   KF    + L F    + L  I    Q  LK     +     + P V  ++A+G  +  ++ +G    C E  CL+  F + L LI+L++ + A+  +V+++ +  EF+ +     ++ L  NK +    DK+Q++  CCG +N  D+          +P SCC NI   C     E     +GC +    ++   +  +A   + +  ++V+ IIF+ C VKS+ +
Sbjct:    3 VEGGMKCVKFLLYVLLLAFCACAVGLIAIGVAVQVVLKQAITHETTAGSLLPVV--IIAVGAFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAVAIAGYVFRDQVKSEFNKSFQQQMQNYLKDNK-TATILDKLQKENNCCGASNYTDWENIPGMAKDRVPDSCCINITVGCGNDFKESTIHTQGCVETIAIWLRKNILLVAAAALGIAFVEVLGIIFSCCLVKSIRS 233          
BLAST of Tetraspanin vs. Ensembl Mouse
Match: Tspan7 (tetraspanin 7 [Source:MGI Symbol;Acc:MGI:1298407])

HSP 1 Score: 63.5438 bits (153), Expect = 2.320e-11
Identity = 48/197 (24.37%), Postives = 85/197 (43.15%), Query Frame = 1
Query:   79 KFTFVFINLIFLLSGITLTVIAALGQSNLKYFAD--ALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDY----IFNNAEIPKSCCGNIKNCTELEAF----------KKGCEKPTEAFV 621
            K   +  + +F ++G+ L  +   G+  L  +    A N    P V  ++  G  ++   L G FA C     +L ++ + L L+ L +  A ++ FV+++ I   F  T     ++  NGN       D +Q+   CCG+ N  ++     F +  IP SCC N  +C  L+            +KGC     +F+
Sbjct:   16 KTLLIIYSFVFWITGVILLAVGVWGKLTLGTYISLIAENSTNAPYV--LIGTGTTIVVFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTFLRTYTDAMQN-YNGNDERSRAVDHVQRSLSCCGVQNYTNWSSSPYFLDHGIPPSCCMNETDCNPLDLHNLTVAATKVNQKGCYDLVTSFM 209          
BLAST of Tetraspanin vs. UniProt/SwissProt
Match: sp|P08962|CD63_HUMAN (CD63 antigen OS=Homo sapiens OX=9606 GN=CD63 PE=1 SV=2)

HSP 1 Score: 69.3218 bits (168), Expect = 1.755e-12
Identity = 56/234 (23.93%), Postives = 108/234 (46.15%), Query Frame = 1
Query:   55 IMSGLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKY--FADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDY----IFNNAEIPKSCCGNIK-----NCTELEAFKKGCEKPTEAFVSYVLTTMAXXXXXXXXXXXXXXXFTFCFVKSMNN 723
            +  G+   KF    + L F    + L  +    Q  L       A    + P V  ++A+G+ +  ++ +G    C E  CL+  F + L LI+L++ +AA+  +V+++ ++ EF+N      ++    N  +    D++Q  +KCCG  N  D+      +   +P SCC N+      N  E    K+GC +    ++   +  +A   + +  ++V+ I+F  C VKS+ +
Sbjct:    3 VEGGMKCVKFLLYVLLLAFCACAVGLIAVGVGAQLVLSQTIIQGATPGSLLPVV--IIAVGVFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAAAIAGYVFRDKVMSEFNNNFRQQMENYPKNNH-TASILDRMQADFKCCGAANYTDWEKIPSMSKNRVPDSCCINVTVGCGINFNEKAIHKEGCVEKIGGWLRKNVLVVAAAALGIAFVEVLGIVFACCLVKSIRS 233          
BLAST of Tetraspanin vs. UniProt/SwissProt
Match: sp|Q76B49|CD63_FELCA (CD63 antigen OS=Felis catus OX=9685 GN=CD63 PE=2 SV=3)

HSP 1 Score: 68.9366 bits (167), Expect = 2.669e-12
Identity = 54/234 (23.08%), Postives = 109/234 (46.58%), Query Frame = 1
Query:   55 IMSGLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIP--IFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDY----IFNNAEIPKSCCGNIKNCTEL-----EAFKKGCEKPTEAFVSYVLTTMAXXXXXXXXXXXXXXXFTFCFVKSMNN 723
            +  G+   KF    + L F    + L  +    Q  L+        P  + P V  ++A+G  +  ++ +G    C E  CL+  F + L LI+L++ + A+  +V+++ ++ EF+       ++    N  +    D++Q+ +KCCG  N  D+    + + A +P SCC N+     +     E  ++GC +    ++   +  +A   + +  ++V+ IIF  C VKS+ +
Sbjct:    3 VEGGMKCVKFLLYVLLLAFCACAVGLIAVGVGAQLVLRQTIVQGATPGSLLPVV--IIAVGAFLFLVAFVGCCGACKENYCLMVTFAIFLSLIMLVEVAVAIAGYVFRDKVMSEFNKDFRQQMQNYPKNNH-TVSIVDRMQEDFKCCGAANYTDWGSIPLMSKARVPDSCCVNVTQGCGISFKVKEIHEEGCVEKIGGWLRSNVLVVAAAALGIAFVEVLGIIFACCLVKSIRS 233          
BLAST of Tetraspanin vs. UniProt/SwissProt
Match: sp|O43657|TSN6_HUMAN (Tetraspanin-6 OS=Homo sapiens OX=9606 GN=TSPAN6 PE=1 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 9.275e-12
Identity = 47/169 (27.81%), Postives = 80/169 (47.34%), Query Frame = 1
Query:   79 KFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVL-AIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYI----FNNAEIPKSCCGNIKNCT 570
            K   +    IF ++G+ L  +   G+ +L+ +   LN     +V FVL A G ++I +   G FA C     +L ++ + L L+ L++  AA+  FV+++ I   F N      K   +         DKIQ    CCG+ +  D+     ++    PKSCC  +++CT
Sbjct:   18 KSVLLIYTFIFWITGVILLAVGIWGKVSLENYFSLLNEKA-TNVPFVLIATGTVIILLGTFGCFATCRASAWMLKLYAMFLTLVFLVELVAAIVGFVFRHEIKNSFKNNYEKALKQYNSTGDYRSHAVDKIQNTLHCCGVTDYRDWTDTNYYSEKGFPKSCC-KLEDCT 184          
BLAST of Tetraspanin vs. UniProt/SwissProt
Match: sp|P28648|CD63_RAT (CD63 antigen OS=Rattus norvegicus OX=10116 GN=Cd63 PE=1 SV=2)

HSP 1 Score: 67.0106 bits (162), Expect = 9.906e-12
Identity = 60/234 (25.64%), Postives = 109/234 (46.58%), Query Frame = 1
Query:   55 IMSGLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKYF--ADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYI----FNNAEIPKSCCGNIK-NC----TELEAFKKGCEKPTEAFVSYVLTTMAXXXXXXXXXXXXXXXFTFCFVKSMNN 723
            +  G+   KF    + L F    + L  I    Q  LK     +     + P V  ++A+G  +  ++ +G    C E  CL+  F + L LI+L++ + A+  +V+++ +  EF  +     ++ L  NK +    DK+Q++ KCCG +N  D+          +P SCC NI   C     E     +GC +   A++   +  +A   + +  ++V+ IIF+ C VKS+ +
Sbjct:    3 VEGGMKCVKFLLYVLLLAFCACAVGLIAIGVAVQVVLKQAITHETTAGSLLPVV--IIAVGAFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAVAIAGYVFRDQVKSEFSKSFQKQMQNYLTDNK-TATILDKLQKENKCCGASNYTDWERIPGMAKDRVPDSCCINITVGCGNDFKESTIHTQGCVETIAAWLRKNVLLVAGAALGIAFVEVLGIIFSCCLVKSIRS 233          
BLAST of Tetraspanin vs. UniProt/SwissProt
Match: sp|Q5RAS5|TSN6_PONAB (Tetraspanin-6 OS=Pongo abelii OX=9601 GN=TSPAN6 PE=2 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 1.012e-11
Identity = 48/169 (28.40%), Postives = 80/169 (47.34%), Query Frame = 1
Query:   79 KFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVL-AIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYI----FNNAEIPKSCCGNIKNCT 570
            K   +    IF ++G+ L  +   G+ +L+ +   LN     +V FVL A G ++I +   G FA C     +L ++ + L LI L++  AA+  FV+++ I   F N      K   +         DKIQ    CCG+ +  D+     ++    PKSCC  +++CT
Sbjct:   18 KSVLLIYTFIFWITGVILLAVGIWGKVSLENYFSLLNEKA-TNVPFVLIATGTVIILLGTFGCFATCRASAWMLKLYAMFLTLIFLVELVAAIVGFVFRHEIKNSFKNNYEKALKQYNSTGDYRSHAVDKIQNTLHCCGVTDYRDWTDTNYYSEKGFPKSCC-KLEDCT 184          
BLAST of Tetraspanin vs. TrEMBL
Match: R4WRU5 (Tetraspanin OS=Riptortus pedestris OX=329032 PE=2 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 2.370e-21
Identity = 66/216 (30.56%), Postives = 107/216 (49.54%), Query Frame = 1
Query:   76 AKFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYIFNNAEIPKSCCGNIKNCTELEAFKKGCEKPTEAFVSYVLTTMAXXXXXXXXXXXXXXXFTFCFVKSMNN 723
            A+F     NL + L G+ +  I        K F + L+  +  S   ++ +G  V  I+  G      E  C L  +GV+L  ILL Q +  V  FVYKN +    +N +   F +A  G++ S +  + IQ+  KCCGL+  N +I +++EIP SCCG   +CT  +AF  GC+      +   L  M +  I + +++++ +IF FC   S+  
Sbjct:    8 ARFVLFIFNLAWALLGLAILGIGIYMNIQGKTFREVLDGQLTVSTILLIVVGGSVFLIAFFGCCGAIREDPCCLNTYGVILSAILLCQVAVGVIAFVYKNQVQTSLNNYIEKEFLNATRGDQESINIVNGIQKDIKCCGLDGPNYWIKHSSEIPPSCCGGKSSCTANDAFSDGCKDKAVTTIVGFLYIMGIAAIAVGVVELLGVIFAFCLASSIKR 223          
BLAST of Tetraspanin vs. TrEMBL
Match: A0A076KUR5 (Tetraspanin OS=Nephila pilipes OX=299642 PE=2 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 2.438e-20
Identity = 54/178 (30.34%), Postives = 101/178 (56.74%), Query Frame = 1
Query:   79 KFTFVFINLIFLLSGITLTVIAALGQS-NLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKD-FFDKIQQKYKCCGLNNANDYIFNNAEIPKSCC-GNIKNCTELEAFKKGCEK 603
            K+    +N +F++ GI L VI AL Q+ ++  F D   +    + T ++ IG  +  ++ LG      E +C++T FG+LL ++ +++ + A T ++Y+     +F+  +     +++  NK      +D++Q K++CCG++ A DY  NN ++PKSCC   I  C+  +A++KGC K
Sbjct:   10 KYLMFIMNFVFVVCGIVLIVIGALVQTQDVTRFLDTKYVT---APTILIVIGCAIFVLAFLGCCGAVRENSCMVTTFGILLFIVFVVEIAGAATAYMYRG----QFEGLLKDNMNNSIAQNKSDAHVIWDEMQSKWECCGVDGAKDYPENNLDVPKSCCNAQIDTCSVADAYQKGCYK 180          
BLAST of Tetraspanin vs. TrEMBL
Match: A0A0A0V5H3 (Tetraspanin (Fragment) OS=Scytodes thoracica OX=1112478 PE=2 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 4.017e-20
Identity = 63/221 (28.51%), Postives = 108/221 (48.87%), Query Frame = 1
Query:   55 IMSGLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYIFNNAEIPKSCCGNI-KNCTELEAFKKGCEKPTEAFVSYVLTTMAXXXXXXXXXXXXXXXFTFCFVKS 714
            +  G+   K      N IF+L GI L V+ AL   +   +   LN     + T V+ +G I+  I+  G      +  C++T FG+LL +I +++ +  +  +VYKN++      +M  +        K  K  ++++Q ++ CCG+    DY+    EIP SCCG   ++C E +AFK GC K    F    L  +    I + +++VI +IF +C   +
Sbjct:    2 VQGGMSVVKVLLFVFNFIFVLCGIALIVVGALAHESKNDYTTFLNSKYVTAPTIVITLGCIIFLIAFFGCCGAVKQNYCMVTTFGILLFVIFIVEIAGGIFAYVYKNDLDGLLKKSMTDSLSQQ---KKDGKQIWNEMQIEFHCCGVFGVEDYLNRTMEIPTSCCGKTAESCNEKDAFKDGCHKKLLDFFGSRLVKVGGAGIGIAVVEVIGMIFAWCLASA 219          
BLAST of Tetraspanin vs. TrEMBL
Match: A0A224XJR8 (Tetraspanin OS=Panstrongylus lignarius OX=156445 PE=3 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 6.193e-20
Identity = 67/219 (30.59%), Postives = 109/219 (49.77%), Query Frame = 1
Query:   76 AKFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYIFNNAEIPKSCCGNIKN--CTELEAFKKGCEKPTEAFVSYVLTTMAXXXXXXXXXXXXXXXFTFCFVKSMNNY 726
            AKF     NLI  L G+ +   + +   + K F D L+  I      ++ +G++V  I+ LG      E +C+LT + V+L  +L++QF+  +T FVY+N I    ++ +N TF+     N  + D  D+IQ   +CCGL+    Y   +  +P SCCG  K   C  +EA+  GC       V   L  +  + I + +I++I +IF FC   S   +
Sbjct:    8 AKFALFAFNLICALLGLVIIAFSVVTNIHGKPFKDMLSGQITLVTIILIVVGVLVFCIAFLGCCGAIRESHCMLTTYAVILSTLLVVQFAIGITAFVYRNKIEDSLNSVINQTFE-----NDKAVDIVDEIQMDLQCCGLDGPIWY--KSVNLPSSCCGRTKGKTCFIIEAYIGGCHDKIIDLVQSSLKLLGAIAIGISVIELICVIFAFCLSSSFKRW 219          
BLAST of Tetraspanin vs. TrEMBL
Match: A0A087TFG6 (Tetraspanin (Fragment) OS=Stegodyphus mimosarum OX=407821 GN=X975_08662 PE=3 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 8.676e-20
Identity = 67/226 (29.65%), Postives = 121/226 (53.54%), Query Frame = 1
Query:   55 IMSGLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNK-GSKDFFDKIQQKYKCCGLNNANDYIFNNAEIPKSCCGN--IKNCTELEAFKKGCEKPTEAFVSYVLTTMAXXXXXXXXXXXXXXXFTFCFVKSMNN 723
            + +G+   K+     N IF++ GI L  I A+   N K F   L+     + T ++ +G I+  ++ LG      E +C++  FG+LL +I +I+ +  +  +V ++     F++ + T   S++   K  +   +D+IQ K+ CCG+++A+DYI NNA IP+SCC     + CTE  A+KKGC K     V   +  +  V I + ++++I +IF +C   ++  
Sbjct:    1 MQAGMSCIKYLMFVFNFIFVVCGIALIAIGAV-VINTKDFTVFLDSKYVTAPTILIIVGCIIFVVAFLGCCGAVKENHCMVMTFGILLFIIFVIEIAGGIAAYVNRSG----FEDFLKTNMNSSIEQQKTDAMKIWDEIQTKWHCCGVDDASDYINNNANIPQSCCKPEITQACTEELAYKKGCFKEMLQHVKATIGKVGGVAIGIAVVELIGVIFAWCLASAIKK 221          
BLAST of Tetraspanin vs. Ensembl Cavefish
Match: zgc:64051 (leukocyte surface antigen CD53-like [Source:NCBI gene;Acc:103036604])

HSP 1 Score: 81.2629 bits (199), Expect = 7.573e-18
Identity = 57/187 (30.48%), Postives = 93/187 (49.73%), Query Frame = 1
Query:   58 MSGLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYIFNNAEIPKSCCGNIKNCTELEAFKKGCEKPTEAF 618
            MS L   K+T   +N IF + G  +  +    +S       A +IP    V  +  +GI +  +S LG      E  CLL  F +LL L++L +   A    +Y++ I K  +  +N + + A+  N GS   +D +Q+ +KCCG+ NA D+  N   +PKSCC   ++C   E + +GC K  + +
Sbjct:    1 MSCLKCLKYTMCVVNFIFFICGAGIFGLGIFLKSTANLSVIAPSIPAVNVVNGLFILGIFITCVSFLGFLGALKENRCLLISFFILLFLVMLAELGVACFLLMYESQIDKTIEGELNKSLEEAMK-NNGSNIEWDTVQKTFKCCGVKNATDWKNN---VPKSCC-KTESCE--EKYNQGCYKKLQDW 180          
BLAST of Tetraspanin vs. Ensembl Cavefish
Match: zgc:64051 (leukocyte surface antigen CD53-like [Source:NCBI gene;Acc:103036604])

HSP 1 Score: 77.411 bits (189), Expect = 1.840e-16
Identity = 54/172 (31.40%), Postives = 86/172 (50.00%), Query Frame = 1
Query:   58 MSGLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYIFNNAEIPKSCCGNIKNCTE 573
            MS L   K+T   +N IF + G  +  +    +S       A +IP    V  +  +GI +  +S LG      E  CLL  F +LL L++L +   A    +Y++ I K  +  +N + + A+  N GS   +D +Q+ +KCCG+ NA D+  N   +PKSCC   ++C E
Sbjct:    1 MSCLKCLKYTMCVVNFIFFICGAGIFGLGIFLKSTANLSVIAPSIPAVNVVNGLFILGIFITCVSFLGFLGALKENRCLLISFFILLFLVMLAELGVACFLLMYESQIDKTIEGELNKSLEEAMK-NNGSNIEWDTVQKTFKCCGVKNATDWKNN---VPKSCC-KTESCEE 167          
BLAST of Tetraspanin vs. Ensembl Cavefish
Match: cd63 (CD63 molecule [Source:NCBI gene;Acc:103032697])

HSP 1 Score: 68.1662 bits (165), Expect = 5.927e-13
Identity = 38/151 (25.17%), Postives = 78/151 (51.66%), Query Frame = 1
Query:  115 LSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYI---FNNAEIPKSCCGNI 558
            L G+ L V+    Q +L             +   ++ +G+++  I+  G      E  C++T+F +LL LI++++   A+  +V+++ +    D+ +    K   NG+K  ++  D+IQ+  KCCG  N++D++    ++  +P SCC N+
Sbjct:   44 LCGLALIVLGVWVQVSLHKTPVIQGASGSAAPLVLIIVGVVIFFIAFFGCCGAWKENYCMVTMFAILLSLIIIVEIGTAIAAYVFRDKLTGLVDDGLRDMIKQYNNGSKEYQEGLDRIQKDLKCCGAANSSDWVNFKPDHTSVPDSCCKNV 194          
BLAST of Tetraspanin vs. Ensembl Cavefish
Match: cd63 (CD63 molecule [Source:NCBI gene;Acc:103032697])

HSP 1 Score: 67.781 bits (164), Expect = 6.567e-13
Identity = 32/117 (27.35%), Postives = 68/117 (58.12%), Query Frame = 1
Query:  217 VLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYI---FNNAEIPKSCCGNI 558
            ++ +G+++  I+  G      E  C++T+F +LL LI++++   A+  +V+++ +    D+ +    K   NG+K  ++  D+IQ+  KCCG  N++D++    ++  +P SCC N+
Sbjct:   57 LIIVGVVIFFIAFFGCCGAWKENYCMVTMFAILLSLIIIVEIGTAIAAYVFRDKLTGLVDDGLRDMIKQYNNGSKEYQEGLDRIQKDLKCCGAANSSDWVNFKPDHTSVPDSCCKNV 173          
BLAST of Tetraspanin vs. Ensembl Cavefish
Match: tspan36 (tetraspanin 36 [Source:NCBI gene;Acc:437021])

HSP 1 Score: 66.2402 bits (160), Expect = 1.827e-12
Identity = 52/206 (25.24%), Postives = 96/206 (46.60%), Query Frame = 1
Query:   64 GLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALN--IPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDY------IFNNAEIPKSCCGNIKNCTELEAFKK-------GCEKPTEAFVSYVLT 636
            G+  +K   + ++LIF  +G  L+ + A    + + F + L     + P+   ++ + +++  I L+G  A   E    L+ F V+L+L+   + +A V  F+Y+  I  + + +M+  F+   +G        D +Q + +CCG+NN  D+        +N+ +P SCC     CT    F K       GCE   E  +  VL+
Sbjct:    4 GILTSKSVLLILSLIFWAAGAALSYVGAYVIKSYRTFDNFLEDKYTLIPA-AIIIGVAVVLFIIGLIGCCATLRESKVGLSFFMVILLLVFAAEVAAFVFGFIYRGKIKGDLEKSMSDVFQK-YDGKNAETAAVDYLQSQLQCCGVNNYTDWSSRPWFTSHNSTVPVSCCKANSTCT--GQFDKPELLNVQGCEAKLEKLLQDVLS 205          
BLAST of Tetraspanin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005284.1 (pep scaffold:Pmarinus_7.0:GL480552:16638:25636:-1 gene:ENSPMAG00000004798.1 transcript:ENSPMAT00000005284.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 63.929 bits (154), Expect = 3.876e-12
Identity = 52/209 (24.88%), Postives = 100/209 (47.85%), Query Frame = 1
Query:   61 SGLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKYFADAL-NIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNG-NKGSKDFFDKIQQKYKCCGLNNANDYIFNNA-----------EIPKSCCGNI-----------KNCTELEAFKKGCEKPTEA 615
             G+   K+  +  NL+F LSG+ L ++ A+ Q ++  F+    N+   P V  ++A+G+++  ++  G      E   ++  F V L LI + + +A +  +++K+    +  + + T  K  +N     +KD  D +Q++ +CCG+ +  D+ FN+             +P SCC N+           KN T +  + KGC +  EA
Sbjct:   13 GGMACIKYLVLLFNLLFWLSGLGLIIVGAMAQMHIGAFSVVTGNLSGAPYV--IIAVGVVIFVVAAFGCCGAHKESYFMVATFSVFLFLIFVAELAAGIGAYIFKD----QLRDVVETKAKVVINDYPTKNKDPVDNMQKELQCCGVVHYQDW-FNSTNWVTSDPNNPNSVPDSCCLNVTSGCGQDDIRFKNATAI-IYTKGCVEKLEA 213          
BLAST of Tetraspanin vs. Ensembl Sea Lamprey
Match: CD151 (CD151 molecule (Raph blood group) [Source:HGNC Symbol;Acc:HGNC:1630])

HSP 1 Score: 63.5438 bits (153), Expect = 5.064e-12
Identity = 51/177 (28.81%), Postives = 86/177 (48.59%), Query Frame = 1
Query:   64 GLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNI---PIFPSVTFVLAI-GIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSK--DFFDKIQQKYKCCGLNNANDYIFNN------AE---IPKSCC 549
            G    K+  +  N IF L+G     + A+G   L    D L++     F +  ++L + G +V+   LLG  A   ER   L  +  LL+LI L++  A +  ++Y+  + +E  + MN T   +       +  D  DK+QQ++KCCG ++ ND+  ++      AE   +P SCC
Sbjct:   13 GTVCLKYVLLVFNFIFWLTGAG---VMAVGVWTLVEKGDYLSLLASSTFAATAYILILAGSVVMLTGLLGCCAVVRERRGCLAAYCGLLLLIFLLEVIAGILAYLYRQQLSEELKSHMNKTIVESYGQPDQQRVTDAMDKLQQEFKCCGSHDWNDWQRSDWVKVAAAEGRLVPDSCC 186          
BLAST of Tetraspanin vs. Ensembl Sea Lamprey
Match: tspan3a (tetraspanin 3a [Source:ZFIN;Acc:ZDB-GENE-030131-7787])

HSP 1 Score: 59.3066 bits (142), Expect = 1.421e-10
Identity = 48/173 (27.75%), Postives = 86/173 (49.71%), Query Frame = 1
Query:  166 KYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKS--ALNGNKGSKDFFDKIQQKYKCCGLNNANDY----IFN---NAEIPKSCC--------GNIKNCTELEAFKKGCEKPTEAFVSYVL 633
            ++F +   + + P V  V+A+GI +  + ++G  A   E  C L+ F +LL++I  ++  AA+  + Y+  +  + + T++  F      + N  S+   D +Q + KCCG+ N  D+     FN   N  +P SCC        GNIK+  ++  + +GCE   E  V  +L
Sbjct:   44 RHFLEDRYVLLAPLV--VVAVGIFLFVVGIVGCCATVRESKCGLSTFFILLLMIFSLEVVAALLGYAYRGQVKDKVERTISQVFNEYNGTDSNTASRAI-DYVQHQLKCCGVKNYLDWKDRPWFNHTGNNSVPISCCRSNVTHCDGNIKHPGDI--YNEGCEAMVEKTVKDIL 211          
BLAST of Tetraspanin vs. Ensembl Sea Lamprey
Match: tspan4a (tetraspanin 4a [Source:ZFIN;Acc:ZDB-GENE-040718-38])

HSP 1 Score: 56.9954 bits (136), Expect = 7.491e-10
Identity = 38/157 (24.20%), Postives = 70/157 (44.59%), Query Frame = 1
Query:  187 NIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALN-----GNKGSKDFFDKIQQKYKCCGLNNANDYI--FNNAEIPKSCCGNIK-NCTELEA---FKKGCEKPTEAFVS 624
            + P F +   +++ G I++ +  +G      E  CLL  F V L++I +++    +  F+Y++ I    D       K  L      GN G  + ++ +Q  + CCG+ N  D+   F +  +P SCC     +C   +    +  GC    EA++ 
Sbjct:   42 SFPSFSAANILISTGTIMMVVGFIGCLGAIKEHKCLLLSFFVCLLIIFVLEMICVILFFMYRDQI----DGHAKDDLKEGLRLYGSEGNVGLTNAWNIVQTDFHCCGVVNHTDWFLAFGDKRVPDSCCMEYSVDCGRDKPNTWWPSGCYDKVEAWIE 194          
BLAST of Tetraspanin vs. Ensembl Sea Lamprey
Match: tspan4a (tetraspanin 4a [Source:ZFIN;Acc:ZDB-GENE-040718-38])

HSP 1 Score: 53.9138 bits (128), Expect = 7.158e-9
Identity = 34/124 (27.42%), Postives = 60/124 (48.39%), Query Frame = 1
Query:  187 NIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNI-VKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDY--IFNNAEIPKSCC 549
            N+P+  +   ++A+G   + +  LG      E  CLL  F VLL+++ L +F   V  FVY+  +  +  D+           GN G  + ++ +  +  CCG++N  D+  +F    +P SCC
Sbjct:   49 NLPLLSAANLLIALGAAAMLLGFLGCLGAIKENPCLLLTFFVLLLVVFLAEFVGGVLFFVYQGKLETRAQDDLRQGLHLYGTEGNVGLTNAWNTLHSEVGCCGVHNHTDWFAVFGERRVPDSCC 172          
BLAST of Tetraspanin vs. Ensembl Nematostella
Match: EDO35283 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SLY4])

HSP 1 Score: 81.2629 bits (199), Expect = 5.399e-18
Identity = 47/164 (28.66%), Postives = 81/164 (49.39%), Query Frame = 1
Query:   79 KFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKS--ALNGNKGSKDFFDKIQQKYKCCGLNNANDYIFNN-----AEIPKSCC 549
            K   VF N++F + GI L  + A  +     F +  ++P       V+A+G+I+  +S  G      E  C+L  F VLL+++L ++ +AAV  + Y+  +  E ++ +    K     +G  G    FD +Q+K KCCG+N   D+  +      + +P SCC
Sbjct:   13 KILVVFFNVLFFIFGIALIAVGAWAEVQYGEFIEVSSVPYATGSRLVIAVGVIIAIVSFFGCLGAWKENRCMLGTFFVLLLILLTLEIAAAVLAYNYRGKVKDEIESDLTKALKGDYGSSGQDGVTKAFDALQEKQKCCGVNGYLDWKASKKYNGTSTVPDSCC 176          
BLAST of Tetraspanin vs. Ensembl Nematostella
Match: EDO43827 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXD6])

HSP 1 Score: 75.0998 bits (183), Expect = 4.745e-16
Identity = 56/189 (29.63%), Postives = 92/189 (48.68%), Query Frame = 1
Query:   61 SGLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALN--GNKGSKDFFDKIQQKYKCCGLNNANDYIFNNAEIPKSCCGNIKNCTELEAFKKGCEKPTEAFV 621
             G+   K+   F N IF LSG+ L ++ A+ +S    F    +  +     F++ IG+IV  +  LG      E  C++T F VLL  I +++ +A    + YK  +    +  + T  K+ ++    K  K   DK+Q ++KCCG  ++ND+ + N+ +PKSC       T    F K C K  E F+
Sbjct:    4 GGMACVKYLLFFFNFIFWLSGLALVIVGAIIKSKYGDFIQVSSSSLASGPVFLIVIGVIVAVVGFLGCCGAYKENYCMVTTFAVLLAFIFVLEIAAGALAYAYKGKL----EGYVRTGLKNGVDHYDKKEYKKAMDKVQGEFKCCGYEDSNDHGYKNSTVPKSC-----KDTNGTVFSKNCVKGLEKFL 183          
BLAST of Tetraspanin vs. Ensembl Nematostella
Match: EDO47592 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RLE8])

HSP 1 Score: 68.1662 bits (165), Expect = 1.787e-13
Identity = 52/185 (28.11%), Postives = 85/185 (45.95%), Query Frame = 1
Query:   79 KFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKS-ALNGNKGSKDFFDKIQQKYKCCGLNNANDYIF------NNAEIPKSCCGNI-KNC----TELEAFKKGC 597
            K+     N IF L G+ L  +    Q  +  + +  ++        ++A+G  +  +S  G      E  CLL  F  LL +IL ++ +     +VY+N + ++    MN T  S    G +G  D +D IQ+K KCCG+N   D+        N++ +P SCC  + K C     E + + KGC
Sbjct:   11 KYMVFLFNFIFFLGGVGLMGVGIYVQLKIGDYVELQSVKYITGSIIIIAVGAFIALVSFFGCCGAIKEHRCLLATFFALLFIILAVEITGTALGYVYRNKVEEQLGKDMNDTIVSYGEKGKEGITDAYDAIQKKEKCCGINGFKDWQRSYYTKGNHSIVPDSCCKTVSKGCGINFKENDIYTKGC 195          
BLAST of Tetraspanin vs. Ensembl Nematostella
Match: EDO30837 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZN5])

HSP 1 Score: 67.781 bits (164), Expect = 2.746e-13
Identity = 44/172 (25.58%), Postives = 80/172 (46.51%), Query Frame = 1
Query:   79 KFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVT---FVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYIF--------NNAEIPKSCCGNIK 561
            KF     N +F L G+++  +    +     F+D + +      T    ++ IG ++  I  +G      E  CLL  FGV+L L+ L++   A+T +++++ I     + ++T  K      +G KD ++ +Q+  KCCG  N  D+           N  +P+SCC + K
Sbjct:   14 KFLLFGFNALFWLMGLSVLAVGIWARIQ---FSDYMKLSSHDYATAAYILIGIGFLIAIIGFIGCCGALKEHTCLLKTFGVILGLLFLVELGTAITGYIFRSEIKTGLSDGLDTALKD--YPEQGFKDAWNDMQKNLKCCGSRNYTDWFMVAWAGPNTKNGSVPESCCKDTK 180          
BLAST of Tetraspanin vs. Ensembl Nematostella
Match: EDO34309 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SPQ7])

HSP 1 Score: 66.2402 bits (160), Expect = 9.857e-13
Identity = 49/183 (26.78%), Postives = 82/183 (44.81%), Query Frame = 1
Query:   76 AKFTFVFINLIFL-LSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYI-----FNNAEIPKSCCGNIKN-CTELEAFK--KGC 597
            AK    F N I+L +S   + V   L QS   Y     +         ++ +G +V+  +L+G+   C E  C L++F +LL+++  ++ +A V   +YKN    E  N +           +G +   DK+Q+   CCG    ND++          +PKSCC +  + C   + F   KGC
Sbjct:    4 AKAFLFFFNFIYLGISAALIYVAVWLMQSYGNYSKITSDTYTIVPAGIIVGVGCLVLVTALVGICGTCRESKCCLSIFFILLLVVFSLEITAGVLGAIYKNQAKDEVKNGLTDAIDHYSKEEQGFQKAVDKLQENLDCCGSTGPNDWVNSTFWKEKHHLPKSCCTHDSDVCFPYDKFHNTKGC 186          
BLAST of Tetraspanin vs. Ensembl Medaka
Match: cd63 (CD63 molecule [Source:NCBI gene;Acc:101174188])

HSP 1 Score: 75.8702 bits (185), Expect = 7.892e-16
Identity = 55/190 (28.95%), Postives = 99/190 (52.11%), Query Frame = 1
Query:   79 KFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYIFNNAE---IPKSCC---------GNIKNCTELEAFKKGCEKPTE 612
            KF+ VF N I  L G+ L V+  + Q +L       ++ +      ++A+G ++  I+  G      E  C++T F VLL LI++I+ +AA+  FV++N I    +  +    ++  NG    K   D+IQ+  KCCG+N+++D+ + +++   +P SCC         G++KN   +  ++KGC    E
Sbjct:   11 KFSLVFFNFICWLCGLALVVVGIMVQVSLHNTLKIKDVSMSGIPIVIIAVGALIFFIAFFGCCGAWKEHYCMVTTFAVLLTLIIIIEIAAAIAGFVFRNKISPIVNEGLTKMIENYKNGTDDFKATVDQIQENLKCCGVNSSSDWSYLDSDGNSVPDSCCINKTKGCGKGSMKNAGTV--YQKGCHDVIE 198          
BLAST of Tetraspanin vs. Ensembl Medaka
Match: cd63 (CD63 molecule [Source:NCBI gene;Acc:101174188])

HSP 1 Score: 75.8702 bits (185), Expect = 9.041e-16
Identity = 55/191 (28.80%), Postives = 99/191 (51.83%), Query Frame = 1
Query:   76 AKFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYIFNNAE---IPKSCC---------GNIKNCTELEAFKKGCEKPTE 612
             KF+ VF N I  L G+ L V+  + Q +L       ++ +      ++A+G ++  I+  G      E  C++T F VLL LI++I+ +AA+  FV++N I    +  +    ++  NG    K   D+IQ+  KCCG+N+++D+ + +++   +P SCC         G++KN   +  ++KGC    E
Sbjct:   10 VKFSLVFFNFICWLCGLALVVVGIMVQVSLHNTLKIKDVSMSGIPIVIIAVGALIFFIAFFGCCGAWKEHYCMVTTFAVLLTLIIIIEIAAAIAGFVFRNKISPIVNEGLTKMIENYKNGTDDFKATVDQIQENLKCCGVNSSSDWSYLDSDGNSVPDSCCINKTKGCGKGSMKNAGTV--YQKGCHDVIE 198          
BLAST of Tetraspanin vs. Ensembl Medaka
Match: tspan36 (tetraspanin-36 [Source:NCBI gene;Acc:101167371])

HSP 1 Score: 67.781 bits (164), Expect = 7.325e-13
Identity = 57/208 (27.40%), Postives = 101/208 (48.56%), Query Frame = 1
Query:   64 GLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLL--GLFAGCS---ERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYI------FNNAEIPKSCC-GNIKNCT-ELEA----FKKGCEKPTEAFVSYVLT 636
            G+  +K   +F++L+F  +G    V+A +G   ++ + D     I      + A+ II I++ +L  GL   CS   E    LT+F +++++I   + +A V TF+Y   I +  +++MN TF         +    D +Q++ +CCG+ N   +        +N  +P SCC  N+  CT  L+     + +GCE   E F+   LT
Sbjct:    4 GVATSKTILLFLSLVFCTAG---GVLAWVGARVIQSY-DTFEGLIQDKQILIPAVAIICISVVMLIFGLVGCCSTLRESKFGLTLFFIIIMVIFAAEVAALVLTFIYHGKINQNLEHSMNDTFSKYTGEEDAATKAVDLLQKQLQCCGVLNYTSWTSTTWFASHNNSVPISCCRSNVSQCTGRLDQPNLIYTEGCEVKLERFLQDALT 207          
BLAST of Tetraspanin vs. Ensembl Medaka
Match: tspan7 (tetraspanin 6 [Source:NCBI gene;Acc:101163337])

HSP 1 Score: 65.4698 bits (158), Expect = 4.204e-12
Identity = 42/164 (25.61%), Postives = 79/164 (48.17%), Query Frame = 1
Query:  100 NLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDF---FDKIQQKYKCCGLNNANDY----IFNNAEIPKSCCGNIKNCT 570
            +LIF  +G+ L  +   G+ +L+ +    +     +   ++  G I++   L G FA C     +L ++ + L L+ L +  A ++ F++++    E    M T +++A+    G+       D IQ+  +CCG+ N  D+     F +  IP SCC +  NCT
Sbjct:   25 SLIFWFTGVVLLAVGVWGKVSLEAYLSLASEGGTNAPYVLIGTGAIIVIFGLFGCFATCRGSPWMLKLYAMFLTLVFLSELVAGISGFIFRH----EIKAVMGTAYENAVRSYNGTNSLSVALDTIQRTLRCCGVKNYTDWKEAPYFISKGIPLSCCKDNGNCT 184          
BLAST of Tetraspanin vs. Ensembl Medaka
Match: tspan7 (tetraspanin 6 [Source:NCBI gene;Acc:101163337])

HSP 1 Score: 65.4698 bits (158), Expect = 4.733e-12
Identity = 42/164 (25.61%), Postives = 79/164 (48.17%), Query Frame = 1
Query:  100 NLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDF---FDKIQQKYKCCGLNNANDY----IFNNAEIPKSCCGNIKNCT 570
            +LIF  +G+ L  +   G+ +L+ +    +     +   ++  G I++   L G FA C     +L ++ + L L+ L +  A ++ F++++    E    M T +++A+    G+       D IQ+  +CCG+ N  D+     F +  IP SCC +  NCT
Sbjct:   25 SLIFWFTGVVLLAVGVWGKVSLEAYLSLASEGGTNAPYVLIGTGAIIVIFGLFGCFATCRGSPWMLKLYAMFLTLVFLSELVAGISGFIFRH----EIKAVMGTAYENAVRSYNGTNSLSVALDTIQRTLRCCGVKNYTDWKEAPYFISKGIPLSCCKDNGNCT 184          
BLAST of Tetraspanin vs. Planmine SMEST
Match: SMESG000020341.1 (SMESG000020341.1)

HSP 1 Score: 392.889 bits (1008), Expect = 2.556e-139
Identity = 225/226 (99.56%), Postives = 226/226 (100.00%), Query Frame = 1
Query:   58 MSGLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYIFNNAEIPKSCCGNIKNCTELEAFKKGCEKPTEAFVSYVLTTMAXXXXXXXXXXXXXXXFTFCFVKSMNNYIRV 735
            MSGLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVLLILILLIQFSAAVTTFVYKNNIVKEFDNTMNTTFK+ALNGNKGSKDFFDKIQQKYKCCGLNNANDYIFNNAEIPKSCCGNIKNCTELEAFKKGCEKPTEAFVSYVLTTMALVVICLCIIDVIQIIFTFCFVKSMNNYIRV
Sbjct:    1 MSGLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVLLILILLIQFSAAVTTFVYKNNIVKEFDNTMNTTFKNALNGNKGSKDFFDKIQQKYKCCGLNNANDYIFNNAEIPKSCCGNIKNCTELEAFKKGCEKPTEAFVSYVLTTMALVVICLCIIDVIQIIFTFCFVKSMNNYIRV 226          
BLAST of Tetraspanin vs. Planmine SMEST
Match: SMESG000009559.1 (SMESG000009559.1)

HSP 1 Score: 83.5741 bits (205), Expect = 6.013e-19
Identity = 54/147 (36.73%), Postives = 83/147 (56.46%), Query Frame = 1
Query:  214 FVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKD---FFDKIQQKYKCCGLNNANDYIFNNAEIPKSCCGNIKNCTELEAFKKGCEKPTEAFVSYVLTTMA 645
            FV+ +G++V  IS+LG+ A CSE  CLL  FG++LILIL++QF+AA+  F+ ++ +       +    K A    K  K    FFD+IQ   KCCG+ + +DY      IP+SCC N  +C    A+  GC    ++ +   L ++A
Sbjct:   26 FVIIVGVLVSLISVLGMAAACSESKCLLATFGMILILILILQFAAAIMAFMARSKMESSIKGLLEKALKKAYTKEKTGKVNTLFFDRIQDDLKCCGVTSVHDYEMIQKPIPESCCPNKISCNNNTAYNNGCSNSMKSAIGRHLLSIA 172          
BLAST of Tetraspanin vs. Planmine SMEST
Match: SMESG000035080.1 (SMESG000035080.1)

HSP 1 Score: 64.6994 bits (156), Expect = 5.613e-12
Identity = 43/155 (27.74%), Postives = 79/155 (50.97%), Query Frame = 1
Query:  208 VTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGN--KGSKDFFDKIQQKYKCCGLNNANDYIFNN--AEIPKSCCG-NIKNC----TELEAFKKGCEKPTEAFVSYVLTTMA 645
            + F++ IGI  IA+SL+G F    E   +L ++  +++ +   Q +     FVYK+   ++    +  + ++ +N    K  + F + +Q+  KCCG+++ NDYI  +  + IP SCC   I  C     +  AF KGC+   + F  + L  ++
Sbjct:   57 LAFIMGIGIFTIALSLVGCFGAYHESQPILGIYFSVMLFLFASQVAVGSLYFVYKD---EKLTTNLKISIQNYMNSKITKQEEIFLNSVQKMLKCCGVDSVNDYIKKDTPSNIPFSCCDYEIHECKAKVEKKNAFTKGCKDKYKEFSQHKLGVIS 208          
BLAST of Tetraspanin vs. Planmine SMEST
Match: SMESG000063435.1 (SMESG000063435.1)

HSP 1 Score: 63.5438 bits (153), Expect = 1.143e-11
Identity = 41/165 (24.85%), Postives = 79/165 (47.88%), Query Frame = 1
Query:  112 LLSGITLTVIAALGQSNLKYFADALNIPIFPSVTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTT---FKSALNGNKGSKDFFDKIQQKYKCCGLNNANDYIFNNAEIPKSCCGNIKNCTELEAFKKGC 597
            ++ G+ L  + A+  +  K     L      +   ++  G+++  ++ LG      E   +L +FGV LI++L+ Q + ++  +VY++N+       MN     +K+    +  ++   D  QQ ++CCG+N+ +D+      +P SCC N   C    AFK  C
Sbjct:   20 VICGLALIALGAVTMTIYKNSTQLLGSSFSNAPIAIIVAGVVIFIVAFLGCCGAIQESKVMLIIFGVCLIVLLIAQIALSIAVYVYRSNVSTIIGKQMNDAVNIYKT--TNDTAARALVDSTQQLFQCCGVNSTSDW---GKTVPNSCCQNNTVCNADTAFKTNC 179          
BLAST of Tetraspanin vs. Planmine SMEST
Match: SMESG000021627.1 (SMESG000021627.1)

HSP 1 Score: 63.5438 bits (153), Expect = 1.347e-11
Identity = 58/202 (28.71%), Postives = 89/202 (44.06%), Query Frame = 1
Query:   76 AKFTFVFINLIFLLSGITLTVIAALGQSNLK-YFADALNIPIFPSVTF---VLAIGIIVIAISLLGLFAGCSERNCLLTVFGVXXXXXXXXQFSAAVTTFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNANDY--------IFNNAEI-PKSCCGNIKNC----TELEAFKKGCEKPTEAFVSYV 630
            AK   +  N++F LSG+ L VI ++  SN K    + L+   F S      VL +GII+  I+  G      E   LL ++ V L+LI+ IQ +  +    YK  I K    T+ T        +K +    D IQQ   CCG+    D+          N +++ PKSCC +   C    +   AF +GC      ++  +
Sbjct:   10 AKCLLIIFNVVFWLSGLILIVIPSIYLSNEKTKIVEKLDK--FNSRGLFIGVLVLGIIIFVIAFCGCCGAVRESKLLLGMYIVFLMLIIFIQVALGIFAIFYKQKIEKSVSKTLKTAITELYGYDKDNDKITDNIQQILSCCGIEGPQDWEKSKWYIEKMNKSQMFPKSCCRDNAECYNKISGATAFSEGCVSRLREYLQRI 209          
The following BLAST results are available for this feature:
BLAST of Tetraspanin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CD633.655e-1323.93CD63 molecule [Source:HGNC Symbol;Acc:HGNC:1692][more]
CD633.655e-1323.93CD63 molecule [Source:HGNC Symbol;Acc:HGNC:1692][more]
CD633.655e-1323.93CD63 molecule [Source:HGNC Symbol;Acc:HGNC:1692][more]
CD633.655e-1323.93CD63 molecule [Source:HGNC Symbol;Acc:HGNC:1692][more]
CD634.422e-1325.39CD63 molecule [Source:HGNC Symbol;Acc:HGNC:1692][more]
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BLAST of Tetraspanin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3
Match NameE-valueIdentityDescription
tsp-72.995e-1324.48Tetraspanin [Source:UniProtKB/TrEMBL;Acc:Q9XVI4][more]
tsp-86.475e-928.86Tetraspanin [Source:UniProtKB/TrEMBL;Acc:Q19983][more]
tsp-65.433e-824.58Tetraspanin [Source:UniProtKB/TrEMBL;Acc:G4RWX0][more]
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BLAST of Tetraspanin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tsp39D1.255e-1525.00gene:FBgn0032943 transcript:FBtr0273404[more]
Tsp42Ea1.659e-1228.57gene:FBgn0029508 transcript:FBtr0086171[more]
Tsp42Ea1.659e-1228.57gene:FBgn0029508 transcript:FBtr0086173[more]
Tsp42Ea1.659e-1228.57gene:FBgn0029508 transcript:FBtr0086172[more]
Tsp29Fb2.468e-1224.24gene:FBgn0032075 transcript:FBtr0079756[more]
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BLAST of Tetraspanin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cd631.604e-1528.74CD63 molecule [Source:ZFIN;Acc:ZDB-GENE-030131-180... [more]
cd631.604e-1528.74CD63 molecule [Source:ZFIN;Acc:ZDB-GENE-030131-180... [more]
zgc:640512.252e-1128.29zgc:64051 [Source:ZFIN;Acc:ZDB-GENE-040426-1349][more]
tspan73.623e-1130.29tetraspanin 7 [Source:ZFIN;Acc:ZDB-GENE-030131-543... [more]
cd151l1.127e-1023.33CD151 antigen, like [Source:ZFIN;Acc:ZDB-GENE-0404... [more]
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BLAST of Tetraspanin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
asmt3.603e-1326.95acetylserotonin O-methyltransferase [Source:Xenbas... [more]
ENSXETT00000022335.13.789e-1326.95tetraspanin-4-like [Source:NCBI gene;Acc:101734690... [more]
asmt4.971e-1326.95acetylserotonin O-methyltransferase [Source:Xenbas... [more]
ENSXETT00000022348.11.593e-1226.95tetraspanin-4-like [Source:NCBI gene;Acc:101734690... [more]
sertad41.834e-1225.00SERTA domain containing 4 [Source:Xenbase;Acc:XB-G... [more]
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BLAST of Tetraspanin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tspan69.663e-1228.31tetraspanin 6 [Source:MGI Symbol;Acc:MGI:1926264][more]
Cd632.153e-1124.79CD63 antigen [Source:MGI Symbol;Acc:MGI:99529][more]
Cd632.153e-1124.79CD63 antigen [Source:MGI Symbol;Acc:MGI:99529][more]
Cd632.153e-1124.79CD63 antigen [Source:MGI Symbol;Acc:MGI:99529][more]
Tspan72.320e-1124.37tetraspanin 7 [Source:MGI Symbol;Acc:MGI:1298407][more]
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BLAST of Tetraspanin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P08962|CD63_HUMAN1.755e-1223.93CD63 antigen OS=Homo sapiens OX=9606 GN=CD63 PE=1 ... [more]
sp|Q76B49|CD63_FELCA2.669e-1223.08CD63 antigen OS=Felis catus OX=9685 GN=CD63 PE=2 S... [more]
sp|O43657|TSN6_HUMAN9.275e-1227.81Tetraspanin-6 OS=Homo sapiens OX=9606 GN=TSPAN6 PE... [more]
sp|P28648|CD63_RAT9.906e-1225.64CD63 antigen OS=Rattus norvegicus OX=10116 GN=Cd63... [more]
sp|Q5RAS5|TSN6_PONAB1.012e-1128.40Tetraspanin-6 OS=Pongo abelii OX=9601 GN=TSPAN6 PE... [more]
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BLAST of Tetraspanin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
R4WRU52.370e-2130.56Tetraspanin OS=Riptortus pedestris OX=329032 PE=2 ... [more]
A0A076KUR52.438e-2030.34Tetraspanin OS=Nephila pilipes OX=299642 PE=2 SV=1[more]
A0A0A0V5H34.017e-2028.51Tetraspanin (Fragment) OS=Scytodes thoracica OX=11... [more]
A0A224XJR86.193e-2030.59Tetraspanin OS=Panstrongylus lignarius OX=156445 P... [more]
A0A087TFG68.676e-2029.65Tetraspanin (Fragment) OS=Stegodyphus mimosarum OX... [more]
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BLAST of Tetraspanin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
zgc:640517.573e-1830.48leukocyte surface antigen CD53-like [Source:NCBI g... [more]
zgc:640511.840e-1631.40leukocyte surface antigen CD53-like [Source:NCBI g... [more]
cd635.927e-1325.17CD63 molecule [Source:NCBI gene;Acc:103032697][more]
cd636.567e-1327.35CD63 molecule [Source:NCBI gene;Acc:103032697][more]
tspan361.827e-1225.24tetraspanin 36 [Source:NCBI gene;Acc:437021][more]
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BLAST of Tetraspanin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000005284.13.876e-1224.88pep scaffold:Pmarinus_7.0:GL480552:16638:25636:-1 ... [more]
CD1515.064e-1228.81CD151 molecule (Raph blood group) [Source:HGNC Sym... [more]
tspan3a1.421e-1027.75tetraspanin 3a [Source:ZFIN;Acc:ZDB-GENE-030131-77... [more]
tspan4a7.491e-1024.20tetraspanin 4a [Source:ZFIN;Acc:ZDB-GENE-040718-38... [more]
tspan4a7.158e-927.42tetraspanin 4a [Source:ZFIN;Acc:ZDB-GENE-040718-38... [more]
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BLAST of Tetraspanin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Tetraspanin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO352835.399e-1828.66Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO438274.745e-1629.63Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO475921.787e-1328.11Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO308372.746e-1325.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO343099.857e-1326.78Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Tetraspanin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cd637.892e-1628.95CD63 molecule [Source:NCBI gene;Acc:101174188][more]
cd639.041e-1628.80CD63 molecule [Source:NCBI gene;Acc:101174188][more]
tspan367.325e-1327.40tetraspanin-36 [Source:NCBI gene;Acc:101167371][more]
tspan74.204e-1225.61tetraspanin 6 [Source:NCBI gene;Acc:101163337][more]
tspan74.733e-1225.61tetraspanin 6 [Source:NCBI gene;Acc:101163337][more]
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BLAST of Tetraspanin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000020341.12.556e-13999.56SMESG000020341.1[more]
SMESG000009559.16.013e-1936.73SMESG000009559.1[more]
SMESG000035080.15.613e-1227.74SMESG000035080.1[more]
SMESG000063435.11.143e-1124.85SMESG000063435.1[more]
SMESG000021627.11.347e-1128.71SMESG000021627.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024093 ID=SMED30024093|Name=Tetraspanin|organism=Schmidtea mediterranea sexual|type=transcript|length=913bp
AAATAATTGACATGGCATTTCACATTTTTGCATTGATTTTATTACATAAG
TTATATAATGTCTGGACTTTTTGCTGCAAAATTTACATTTGTCTTTATTA
ATTTAATATTTTTGTTATCAGGGATCACACTGACAGTTATAGCTGCTCTG
GGACAATCTAATTTGAAGTATTTTGCAGATGCCTTGAATATACCTATTTT
TCCATCAGTTACTTTTGTTTTGGCTATTGGGATCATAGTAATTGCAATAT
CTCTTTTGGGATTATTTGCGGGATGCTCGGAAAGAAATTGCCTTCTTACT
GTGTTCGGAGTACTTCTGATTCTCATACTACTGATCCAATTTTCCGCGGC
CGTCACCACGTTCGTGTACAAGAACAATATTGTGAAAGAATTTGACAATA
CGATGAATACAACATTTAAAAGCGCACTGAATGGAAACAAAGGTTCAAAA
GATTTTTTCGATAAAATCCAGCAAAAATACAAATGTTGCGGCTTGAATAA
TGCGAATGATTATATCTTTAATAATGCCGAAATACCAAAAAGTTGTTGTG
GAAACATAAAAAATTGTACAGAATTAGAAGCCTTCAAAAAGGGGTGCGAA
AAACCAACGGAAGCATTTGTGTCGTACGTTTTAACAACCATGGCTTTGGT
GGTCATTTGCCTTTGCATTATTGATGTTATTCAAATTATTTTCACATTTT
GTTTTGTGAAATCCATGAATAATTATATTCGAGTATAAAAGAAAAACTCC
AATTTAGTTCCGAATGAATTATAATTTTATAAATTTCTCATTTATTGATA
TTACTTATGGTTCTTGATTTTTTTTTTAAATCAAAATGTTTGAATTGTTA
TTATTAATTTAGATTTTAAACAAAACAATTGTTGAAACTCATTTATAATA
AATTTATTTAAAA
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protein sequence of SMED30024093-orf-1

>SMED30024093-orf-1 ID=SMED30024093-orf-1|Name=SMED30024093-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=227bp
MSGLFAAKFTFVFINLIFLLSGITLTVIAALGQSNLKYFADALNIPIFPS
VTFVLAIGIIVIAISLLGLFAGCSERNCLLTVFGVLLILILLIQFSAAVT
TFVYKNNIVKEFDNTMNTTFKSALNGNKGSKDFFDKIQQKYKCCGLNNAN
DYIFNNAEIPKSCCGNIKNCTELEAFKKGCEKPTEAFVSYVLTTMALVVI
CLCIIDVIQIIFTFCFVKSMNNYIRV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000026gut
PLANA:0000070intestinal phagocyte
PLANA:0002056medial muscle cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR000301Tetraspanin
IPR018499Tetraspanin/Peripherin
IPR008952Tetraspanin_EC2_sf
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000301TetraspaninPRINTSPR00259TMFOURcoord: 47..73
score: 18.12
coord: 9..32
score: 21.19
coord: 74..102
score: 30.96
coord: 194..220
score: 16.0
IPR000301TetraspaninPIRSFPIRSF002419Tetraspanincoord: 2..225
e-value: 8.2E-35
score: 117.8
IPR018499Tetraspanin/PeripherinPFAMPF00335Tetraspanincoord: 8..218
e-value: 4.9E-34
score: 118.0
IPR008952Tetraspanin, EC2 domain superfamilyGENE3DG3DSA:1.10.1450.10coord: 103..188
e-value: 7.3E-13
score: 50.1
IPR008952Tetraspanin, EC2 domain superfamilySUPERFAMILYSSF48652Tetraspanincoord: 102..182
NoneNo IPR availablePANTHERPTHR19282:SF39LEUKOCYTE SURFACE ANTIGEN CD53coord: 3..216
NoneNo IPR availablePANTHERPTHR19282TETRASPANINcoord: 3..216
NoneNo IPR availableCDDcd03127tetraspanin_LELcoord: 102..192
e-value: 7.10336E-21
score: 81.3966
NoneNo IPR availableTMHMMTMhelixcoord: 51..73
NoneNo IPR availableTMHMMTMhelixcoord: 9..31
NoneNo IPR availableTMHMMTMhelixcoord: 80..102
NoneNo IPR availableTMHMMTMhelixcoord: 195..217