Phosphatidylinositol-glycan biosynthesis class W protein

Overview
NamePhosphatidylinositol-glycan biosynthesis class W protein
Smed IDSMED30024079
Length (bp)1455
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Phosphatidylinositol-glycan biosynthesis class W protein (SMED30024079) t-SNE clustered cells

Violin plots show distribution of expression levels for Phosphatidylinositol-glycan biosynthesis class W protein (SMED30024079) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Phosphatidylinositol-glycan biosynthesis class W protein (SMED30024079) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Phosphatidylinositol-glycan biosynthesis class W protein (SMED30024079) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30024079

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
posterior sideSMED30024079h1SMcG0013686 dd_Smed_v4_9579_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30024079h1SMcG0013686 dd_Smed_v4_9579_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Human
Match: PIGW (phosphatidylinositol glycan anchor biosynthesis class W [Source:HGNC Symbol;Acc:HGNC:23213])

HSP 1 Score: 140.584 bits (353), Expect = 7.356e-36
Identity = 144/497 (28.97%), Postives = 229/497 (46.08%), Query Frame = 3 
Query:   54 SSKLNHESFISNLNGTTINENYLLISLPILLNTCMSFLSV-NLFQLGFGKI-KGNLVVNYLMVIIPFILSITIFSNYLLQITLTV---AATXXXXXXXXXXXXXXXXYLTEIR----LTLHSASNKRESWLYISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGL----------LDQIKVGSFTKXXXXXXXXXXXXM-RTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVS----IITKYFRFTQRIFGIITMIMAA---SILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAIL--TRKFRKSL---AVFAALATIGLYISQCFLHKDI--VSRRAANFGYVLWTTSSFCFCLILFIISDILINWLE-----------------------DRANFVNDTE--NPDIYCVV---NRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIY 1358
            S K   E+F+SNLNGTT+ E    +  P     C  FL + + +   F    K   + +++++I+P + ++TI+++++L   L V    A     +           +L  +     ++L S  N       IS  R +    T I ILAVD  +FPRRFAKTE++G G MD GVG F     +          ++  K+  FT        LV   + R  ++K   YQEH +EYG HWNFF T+  +KL++    II    +      GI  +   A   + L+  +LYG+        D    R   LL AN+EG+IS +GY+ + + G   G  +   R   K L   A F  LA I L+IS   +  ++  VSRR AN  + +W  +S    L   ++ DI++++ +                          + V + E   P + C++   NR+ + FFLLSNI TGL N+ + T+  + L ++  + +Y
Sbjct:    2 SEKQMKEAFVSNLNGTTVLEITQGLCFPAFCILCRGFLIIFSQYLCSFSPTWKTRFLTDFVVLIVPMVATLTIWASFILLELLGVIIFGAGLLYQIYRRRTCYARLPFLKILEKFLNISLESEYNPA-----ISCFRVITSAFTAIAILAVDFPLFPRRFAKTELYGTGAMDFGVGGFVFGSAMVCLEVRRRKYMEGSKLHYFTNSLYSVWPLVFLGIGRLAIIKSIGYQEHLTEYGVHWNFFFTIIVVKLITPLLLIIFPLNKSWIIALGITVLYQLALDFTSLKRLILYGT--------DGSGTRV-GLLNANREGIISTLGYVAIHMAGVQTGLYMHKNRSHIKDLIKVACFLLLAAISLFISLYVVQVNVEAVSRRMANLAFCIWIVASSLILLSSLLLGDIILSFAKFLIKGALVPCSWKLIQSPVTNKKHSESLVPEAERMEPSL-CLITALNRKQLIFFLLSNITTGLINLMVDTLHSSTLWALFVVNLY 483
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Human
Match: PIGW (phosphatidylinositol glycan anchor biosynthesis class W [Source:HGNC Symbol;Acc:HGNC:23213])

HSP 1 Score: 140.584 bits (353), Expect = 7.356e-36
Identity = 144/497 (28.97%), Postives = 229/497 (46.08%), Query Frame = 3 
Query:   54 SSKLNHESFISNLNGTTINENYLLISLPILLNTCMSFLSV-NLFQLGFGKI-KGNLVVNYLMVIIPFILSITIFSNYLLQITLTV---AATXXXXXXXXXXXXXXXXYLTEIR----LTLHSASNKRESWLYISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGL----------LDQIKVGSFTKXXXXXXXXXXXXM-RTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVS----IITKYFRFTQRIFGIITMIMAA---SILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAIL--TRKFRKSL---AVFAALATIGLYISQCFLHKDI--VSRRAANFGYVLWTTSSFCFCLILFIISDILINWLE-----------------------DRANFVNDTE--NPDIYCVV---NRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIY 1358
            S K   E+F+SNLNGTT+ E    +  P     C  FL + + +   F    K   + +++++I+P + ++TI+++++L   L V    A     +           +L  +     ++L S  N       IS  R +    T I ILAVD  +FPRRFAKTE++G G MD GVG F     +          ++  K+  FT        LV   + R  ++K   YQEH +EYG HWNFF T+  +KL++    II    +      GI  +   A   + L+  +LYG+        D    R   LL AN+EG+IS +GY+ + + G   G  +   R   K L   A F  LA I L+IS   +  ++  VSRR AN  + +W  +S    L   ++ DI++++ +                          + V + E   P + C++   NR+ + FFLLSNI TGL N+ + T+  + L ++  + +Y
Sbjct:    2 SEKQMKEAFVSNLNGTTVLEITQGLCFPAFCILCRGFLIIFSQYLCSFSPTWKTRFLTDFVVLIVPMVATLTIWASFILLELLGVIIFGAGLLYQIYRRRTCYARLPFLKILEKFLNISLESEYNPA-----ISCFRVITSAFTAIAILAVDFPLFPRRFAKTELYGTGAMDFGVGGFVFGSAMVCLEVRRRKYMEGSKLHYFTNSLYSVWPLVFLGIGRLAIIKSIGYQEHLTEYGVHWNFFFTIIVVKLITPLLLIIFPLNKSWIIALGITVLYQLALDFTSLKRLILYGT--------DGSGTRV-GLLNANREGIISTLGYVAIHMAGVQTGLYMHKNRSHIKDLIKVACFLLLAAISLFISLYVVQVNVEAVSRRMANLAFCIWIVASSLILLSSLLLGDIILSFAKFLIKGALVPCSWKLIQSPVTNKKHSESLVPEAERMEPSL-CLITALNRKQLIFFLLSNITTGLINLMVDTLHSSTLWALFVVNLY 483
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Human
Match: PIGW (phosphatidylinositol glycan anchor biosynthesis class W [Source:HGNC Symbol;Acc:HGNC:23213])

HSP 1 Score: 140.584 bits (353), Expect = 7.356e-36
Identity = 144/497 (28.97%), Postives = 229/497 (46.08%), Query Frame = 3 
Query:   54 SSKLNHESFISNLNGTTINENYLLISLPILLNTCMSFLSV-NLFQLGFGKI-KGNLVVNYLMVIIPFILSITIFSNYLLQITLTV---AATXXXXXXXXXXXXXXXXYLTEIR----LTLHSASNKRESWLYISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGL----------LDQIKVGSFTKXXXXXXXXXXXXM-RTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVS----IITKYFRFTQRIFGIITMIMAA---SILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAIL--TRKFRKSL---AVFAALATIGLYISQCFLHKDI--VSRRAANFGYVLWTTSSFCFCLILFIISDILINWLE-----------------------DRANFVNDTE--NPDIYCVV---NRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIY 1358
            S K   E+F+SNLNGTT+ E    +  P     C  FL + + +   F    K   + +++++I+P + ++TI+++++L   L V    A     +           +L  +     ++L S  N       IS  R +    T I ILAVD  +FPRRFAKTE++G G MD GVG F     +          ++  K+  FT        LV   + R  ++K   YQEH +EYG HWNFF T+  +KL++    II    +      GI  +   A   + L+  +LYG+        D    R   LL AN+EG+IS +GY+ + + G   G  +   R   K L   A F  LA I L+IS   +  ++  VSRR AN  + +W  +S    L   ++ DI++++ +                          + V + E   P + C++   NR+ + FFLLSNI TGL N+ + T+  + L ++  + +Y
Sbjct:    2 SEKQMKEAFVSNLNGTTVLEITQGLCFPAFCILCRGFLIIFSQYLCSFSPTWKTRFLTDFVVLIVPMVATLTIWASFILLELLGVIIFGAGLLYQIYRRRTCYARLPFLKILEKFLNISLESEYNPA-----ISCFRVITSAFTAIAILAVDFPLFPRRFAKTELYGTGAMDFGVGGFVFGSAMVCLEVRRRKYMEGSKLHYFTNSLYSVWPLVFLGIGRLAIIKSIGYQEHLTEYGVHWNFFFTIIVVKLITPLLLIIFPLNKSWIIALGITVLYQLALDFTSLKRLILYGT--------DGSGTRV-GLLNANREGIISTLGYVAIHMAGVQTGLYMHKNRSHIKDLIKVACFLLLAAISLFISLYVVQVNVEAVSRRMANLAFCIWIVASSLILLSSLLLGDIILSFAKFLIKGALVPCSWKLIQSPVTNKKHSESLVPEAERMEPSL-CLITALNRKQLIFFLLSNITTGLINLMVDTLHSSTLWALFVVNLY 483
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Human
Match: PIGW (phosphatidylinositol glycan anchor biosynthesis class W [Source:HGNC Symbol;Acc:HGNC:23213])

HSP 1 Score: 140.584 bits (353), Expect = 7.356e-36
Identity = 144/497 (28.97%), Postives = 229/497 (46.08%), Query Frame = 3 
Query:   54 SSKLNHESFISNLNGTTINENYLLISLPILLNTCMSFLSV-NLFQLGFGKI-KGNLVVNYLMVIIPFILSITIFSNYLLQITLTV---AATXXXXXXXXXXXXXXXXYLTEIR----LTLHSASNKRESWLYISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGL----------LDQIKVGSFTKXXXXXXXXXXXXM-RTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVS----IITKYFRFTQRIFGIITMIMAA---SILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAIL--TRKFRKSL---AVFAALATIGLYISQCFLHKDI--VSRRAANFGYVLWTTSSFCFCLILFIISDILINWLE-----------------------DRANFVNDTE--NPDIYCVV---NRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIY 1358
            S K   E+F+SNLNGTT+ E    +  P     C  FL + + +   F    K   + +++++I+P + ++TI+++++L   L V    A     +           +L  +     ++L S  N       IS  R +    T I ILAVD  +FPRRFAKTE++G G MD GVG F     +          ++  K+  FT        LV   + R  ++K   YQEH +EYG HWNFF T+  +KL++    II    +      GI  +   A   + L+  +LYG+        D    R   LL AN+EG+IS +GY+ + + G   G  +   R   K L   A F  LA I L+IS   +  ++  VSRR AN  + +W  +S    L   ++ DI++++ +                          + V + E   P + C++   NR+ + FFLLSNI TGL N+ + T+  + L ++  + +Y
Sbjct:    2 SEKQMKEAFVSNLNGTTVLEITQGLCFPAFCILCRGFLIIFSQYLCSFSPTWKTRFLTDFVVLIVPMVATLTIWASFILLELLGVIIFGAGLLYQIYRRRTCYARLPFLKILEKFLNISLESEYNPA-----ISCFRVITSAFTAIAILAVDFPLFPRRFAKTELYGTGAMDFGVGGFVFGSAMVCLEVRRRKYMEGSKLHYFTNSLYSVWPLVFLGIGRLAIIKSIGYQEHLTEYGVHWNFFFTIIVVKLITPLLLIIFPLNKSWIIALGITVLYQLALDFTSLKRLILYGT--------DGSGTRV-GLLNANREGIISTLGYVAIHMAGVQTGLYMHKNRSHIKDLIKVACFLLLAAISLFISLYVVQVNVEAVSRRMANLAFCIWIVASSLILLSSLLLGDIILSFAKFLIKGALVPCSWKLIQSPVTNKKHSESLVPEAERMEPSL-CLITALNRKQLIFFLLSNITTGLINLMVDTLHSSTLWALFVVNLY 483
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Human
Match: PIGW (phosphatidylinositol glycan anchor biosynthesis class W [Source:HGNC Symbol;Acc:HGNC:23213])

HSP 1 Score: 100.138 bits (248), Expect = 4.619e-23
Identity = 80/255 (31.37%), Postives = 124/255 (48.63%), Query Frame = 3 
Query:   54 SSKLNHESFISNLNGTTINENYLLISLPILLNTCMSFLSV-NLFQLGFGKI-KGNLVVNYLMVIIPFILSITIFSNYLLQITLTV---AATXXXXXXXXXXXXXXXXYLTEIR----LTLHSASNKRESWLYISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYG------------LLDQIKVGSFTKXXXXXXXXXXXXM-RTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVS 752
            S K   E+F+SNLNGTT+ E    +  P     C  FL + + +   F    K   + +++++I+P + ++TI+++++L   L V    A     +           +L  +     ++L S  N       IS  R +    T I ILAVD  +FPRRFAKTE++G G MD GVG F  ++G             ++  K+  FT        LV   + R  ++K   YQEH +EYG HWNFF T+  +KL++
Sbjct:    2 SEKQMKEAFVSNLNGTTVLEITQGLCFPAFCILCRGFLIIFSQYLCSFSPTWKTRFLTDFVVLIVPMVATLTIWASFILLELLGVIIFGAGLLYQIYRRRTCYARLPFLKILEKFLNISLESEYNPA-----ISCFRVITSAFTAIAILAVDFPLFPRRFAKTELYGTGAMDFGVGGF--VFGSAMVCLEVRRRKYMEGSKLHYFTNSLYSVWPLVFLGIGRLAIIKSIGYQEHLTEYGVHWNFFFTIIVVKLIT 249
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Celegans
Match: hpo-20 (Phosphatidylinositol-glycan biosynthesis class W protein [Source:UniProtKB/TrEMBL;Acc:D6RYD7])

HSP 1 Score: 124.405 bits (311), Expect = 1.406e-30
Identity = 98/361 (27.15%), Postives = 159/361 (44.04%), Query Frame = 3 
Query:  405 RESW-----------LYISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGLLDQIKVGSFTKXXXXXXXXXXXXM------------RTVLVKYGNYQEHESEYGTHWNFFLTMAALKLV--SIITKYFRFT-QRIFGIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAILTRK-------FRKSLAVFA---ALATIGLYISQCFLHKDIVSRRAANFGYVL--WTTSSFCFCLILFIISDILINWLEDRANFVNDTE-----NPDIYCVVNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIY 1358
            R+SW            +++  R L +++ T+ ILAVD  +FPR++AKTE FG   MD+GV  F     L  ++     +             +            R VL++   Y +H +EYG HWNFF TMAA++++  SI+ + +      IFGI    M  S            L+ +  D   +R ++L+ AN EGL SL+GY+ ++     +G  + +         R+   +FA    L  I +   +C    D   RR  N  Y+    T  +F   + L I     + W      F N  +     +P +   +NR ++ FFL+SN+LTGL N+++         +   L+ Y
Sbjct:  124 RQSWSRFVEEQHKPTQFVTYFRSLTMILVTLAILAVDFPIFPRKYAKTEKFGHSMMDVGVAAFLFQAALGSRMAKSPSSGRQLSHHSPRPWYLSSTFLLFALGAGRAVLLEVIGYPQHVTEYGVHWNFFFTMAAIRILSSSILPRCYPLVLSVIFGICHQTMLKST----------GLQAWILDESANRRENLITANAEGLTSLMGYLTIFYASLAIGEFMAKTSIRIKSWIRRCFQLFALSLILFFIQIAAEKCV---DPPCRRVVNATYIFSQLTLMTFATGVCLSIQMFNTVAWCSSFPQFSNGEDPYSVVSPCLSDSINRHSLLFFLVSNVLTGLVNLSVDAHRQPPHIAFPILLAY 471
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Celegans
Match: hpo-20 (Phosphatidylinositol-glycan biosynthesis class W protein [Source:UniProtKB/TrEMBL;Acc:D6RYD7])

HSP 1 Score: 56.9954 bits (136), Expect = 5.138e-9
Identity = 58/216 (26.85%), Postives = 97/216 (44.91%), Query Frame = 3 
Query:  729 MAALKLVS--IITKYFRFT-QRIFGIITMIMAASI-LQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAILTRK-------FRKSLAVFA---ALATIGLYISQCFLHKDIVSRRAANFGYVL--WTTSSFCFCLILFIISDILINWLEDRANFVNDTE-----NPDIYCVVNRQAMAFFLLSNILTGLTNVTIKT 1313
            MAA++++S  I+ + +      IFGI    M  S  LQ ++L           D   +R ++L+ AN EGL SL+GY+ ++     +G  + +         R+   +FA    L  I +   +C    D   RR  N  Y+    T  +F   + L I     + W      F N  +     +P +   +NR ++ FFL+SN+LTGL N+++  
Sbjct:    1 MAAIRILSSSILPRCYPLVLSVIFGICHQTMLKSTGLQAWIL-----------DESANRRENLITANAEGLTSLMGYLTIFYASLAIGEFMAKTSIRIKSWIRRCFQLFALSLILFFIQIAAEKCV---DPPCRRVVNATYIFSQLTLMTFATGVCLSIQMFNTVAWCSSFPQFSNGEDPYSVVSPCLSDSINRHSLLFFLVSNVLTGLVNLSVDA 202
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Fly
Match: PIG-Wb (gene:FBgn0035265 transcript:FBtr0334076)

HSP 1 Score: 88.9669 bits (219), Expect = 1.313e-18
Identity = 101/354 (28.53%), Postives = 161/354 (45.48%), Query Frame = 3 
Query:  423 ISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGLLDQIKVGSFTKXXXXXXXXXXXXM-----RTVLVKYGNYQEHESEYGTHWNFFLTMAALK----LVSIIT-KYFRFTQRIFGIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAILTRK-------FRKSLAVFAALATIGLYISQCFLHKDI-VSRRAANFGYVLW------TTSSFCFCLILFIISDIL---INWLEDRANFVNDTEN----------P-DIYCVVNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIYTILV 1370
            +S +R L  ++T +CILA+D   F R + K+  FG   MD G+GLF     ++ + +   ++ L    I   L ++     RTV +   +Y +   EYG H N F T+   K    LVSI+  K        FG++       I Q+ L      +  +  +  V+R+ S L AN+EGL+SL G++ L+L    +   +  K       F + L     L  I L+ S      +I +SR   N GYV+W      TT    F  I F I+ ++    N  ED    +   E           P  I   +N+  + FFL++NILTG+ N+ ++  + +  ES+  L++Y  L 
Sbjct:  147 LSILRALTHLITAVCILAIDFGSFYRPYRKSRQFGAKLMDTGIGLFVFTMAMVSR-RTRHWSDLRRSVIYSALPLILLGLARTVSILTLSYGQDAHEYGQHLNAFFTLGFTKFLGSLVSILARKDLHLLPLGFGLLV------IHQFGL--SVLGISDYVMNEDVERS-SFLNANREGLVSLPGFVGLFLLSIYVNRWMVAKSLLTYSEFVRKLRRLFYLVLI-LWTSFVISAYEIGISRVTCNLGYVIWMLAIGSTTLCASFGAIDFAINSVMPWDPNPWEDHEKGLLSGEAVVKKRGGSVVPFTISQALNKNGLTFFLVANILTGMVNIFLRPEDRSHSESVIILLVYMFLA 489
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Fly
Match: PIG-Wb (gene:FBgn0035265 transcript:FBtr0072796)

HSP 1 Score: 88.9669 bits (219), Expect = 1.313e-18
Identity = 101/354 (28.53%), Postives = 161/354 (45.48%), Query Frame = 3 
Query:  423 ISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGLLDQIKVGSFTKXXXXXXXXXXXXM-----RTVLVKYGNYQEHESEYGTHWNFFLTMAALK----LVSIIT-KYFRFTQRIFGIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAILTRK-------FRKSLAVFAALATIGLYISQCFLHKDI-VSRRAANFGYVLW------TTSSFCFCLILFIISDIL---INWLEDRANFVNDTEN----------P-DIYCVVNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIYTILV 1370
            +S +R L  ++T +CILA+D   F R + K+  FG   MD G+GLF     ++ + +   ++ L    I   L ++     RTV +   +Y +   EYG H N F T+   K    LVSI+  K        FG++       I Q+ L      +  +  +  V+R+ S L AN+EGL+SL G++ L+L    +   +  K       F + L     L  I L+ S      +I +SR   N GYV+W      TT    F  I F I+ ++    N  ED    +   E           P  I   +N+  + FFL++NILTG+ N+ ++  + +  ES+  L++Y  L 
Sbjct:  147 LSILRALTHLITAVCILAIDFGSFYRPYRKSRQFGAKLMDTGIGLFVFTMAMVSR-RTRHWSDLRRSVIYSALPLILLGLARTVSILTLSYGQDAHEYGQHLNAFFTLGFTKFLGSLVSILARKDLHLLPLGFGLLV------IHQFGL--SVLGISDYVMNEDVERS-SFLNANREGLVSLPGFVGLFLLSIYVNRWMVAKSLLTYSEFVRKLRRLFYLVLI-LWTSFVISAYEIGISRVTCNLGYVIWMLAIGSTTLCASFGAIDFAINSVMPWDPNPWEDHEKGLLSGEAVVKKRGGSVVPFTISQALNKNGLTFFLVANILTGMVNIFLRPEDRSHSESVIILLVYMFLA 489
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Fly
Match: PIG-Wa (gene:FBgn0031422 transcript:FBtr0077768)

HSP 1 Score: 78.9518 bits (193), Expect = 2.016e-15
Identity = 81/323 (25.08%), Postives = 138/323 (42.72%), Query Frame = 3 
Query:  456 TTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGLLDQIKVGSFTKXXXXXXXXXXXXM----RTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVSIITKYFRFTQRIFGIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAILT-----------RKFRKSLAVFAALATIGLYISQCFLHKDIVSRRAANFGYVLWTTSS-------FCFCLILFIISDILINWLEDRANFVNDTENPDIYCVVNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIY 1358
            T   ILAVD   +P  + K+  FG   MD+G+GLF +  GL+   +  S T L  +   ++  ++    RT+++   +Y + E+EYG H N F  +   KL+   + Y         ++ + +    L   +L     L +F   +     +    AN+EGL SL G + L+L         T           RK +  L  F A+    L     FL    ++R   +FGYV+W  +        + F   L ++   + +    R +   +   P     +N   +  F++SN LTG+ N+++     + LES+  L IY
Sbjct:  129 TCAAILAVDFPSYPTDYRKSRTFGAAVMDMGIGLFVVTMGLVSH-RARSITDLKKLRRSVIPLLVLGLARTIVITLIDYHQDETEYGRHLNAFFILGFTKLLG--SCYSLLVNSDEQLLALAIGLLFLHEVVL--QLGLSEFVMSSAPH--EGFFSANREGLSSLHGCVALYLLSIYFAKWYTSRDSLTYQGLIRKLKSIL--FVAILCWKLVFISSFLTG--IARVTFSFGYVIWIFAVSLSLIVIYAFFFELKLVRPDICSKKSIRDDGQKNISLPTFVESLNMNGLTHFMISNFLTGMVNMSLNPGHRSDLESVVILSIY 440
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Zebrafish
Match: pigw (phosphatidylinositol glycan anchor biosynthesis, class W [Source:NCBI gene;Acc:100136853])

HSP 1 Score: 155.606 bits (392), Expect = 1.562e-41
Identity = 144/488 (29.51%), Postives = 242/488 (49.59%), Query Frame = 3 
Query:   75 SFISNLNGTTINENYLLISLPILLNTCMSFLSVNLFQLGFGKIKG--------NLVVNYLMVIIPFILSITIFSNYLLQITLTVAATXXXXXXXXXXXXXXXXYLTEIRLTLHSASNKRESWLYISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGLLDQIKVGS----------FTKXXXXXXXXXXXXMRTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVSIITKYFRFTQRIFGIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAIL--TRKFRKSLAVFAA---LATIGLYISQCFLHKDI--VSRRAANFGYVLWTTSSFCFCLILFIISDILI--------------NWLEDRANFVNDTENPDI--YCV---VNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIYTIL--VLITTPHINGI 1400
            +F+SNLNGTT+ E    +SL   L   + FLS  LF + F   KG        +L++++  +I+P +LS T  S+ L  + + +A   F +L       K+   L         +  + +   +++  R L+ + T I ILAVD  VFPRR+AKTE +G G MD GVG + +   L+     G             +  S+  L++LG +R   VK   Y EH +EYG HWNFF T+A +++V+       F     G + +I+  +   Y ++  + +L+ F   N  +R+   L+AN+EG+ S++GYI +++ G  +G  L  +R   K          L + GL++      ++I  VSRR AN  + +WT +   F L    I+D+++              +W  ++    ++    D+   C+   VNR  + +FLL+N+LTGLTNV + TM    + +++ L+ Y  +  ++I   H+N I
Sbjct:    9 AFVSNLNGTTLTE----VSLGSFLAP-VCFLSRGLFLIVFHLGKGVLPFSWGAHLMLDFTTIIMPMVLSCTALSDILHFVIMGIAVVDFAVLYLVYKRKKNPSSLKSTIDKFIQSRAESKMVPFVTIFRVLVNVKTAISILAVDFSVFPRRYAKTETYGTGVMDFGVGAYVMANALVCPEARGKNIQGSKISHVLKQAVSVWPLILLGFVRLASVKSSGYHEHVTEYGVHWNFFFTLAIVRVVASALLAL-FPVNCSGFLALIICGT---YQVILETTDLKSFLIHN-TERS-GFLLANREGVFSVVGYIAIYMAGVHVGCYLMQSRNLVKDWIRVIRNLLLTSFGLFLVLYVCQRNIEPVSRRMANLPFCIWTVAQALFFLSCISIADVVLLFAKVVSNFSLVPTSWCPNQNKKADEKTRRDMDGLCLIQAVNRNQLLYFLLANVLTGLTNVFVDTMNSRDVLAVSVLLWYMFMNSLVIFILHVNKI 485
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Xenopus
Match: ndrg1 (N-myc downstream regulated 1 [Source:Xenbase;Acc:XB-GENE-993273])

HSP 1 Score: 154.066 bits (388), Expect = 9.979e-41
Identity = 138/478 (28.87%), Postives = 230/478 (48.12%), Query Frame = 3 
Query:   54 SSKLNHESFISNLNGTTINENYLLISLPILLNTCMSF--LSVNLFQLGFGKI----KGNLVVNYLMVIIPFILSITIFSN--YLLQITLTVAATXXXXXXXXXXXXXXXXYLTEIRLTLHSASNKRESWLYISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGLL-------DQIKVGSFTKXXXXXXXXXXXXM----RTVLVKYGNYQEHESEYGTHWNFFLTMAALK-LVSIITKYFRFTQRIFGIITMI------MAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAILTRK---FRKSLAVFAALATIGLYISQCF----LHKDIVSRRAANFGYVLWTTSSFCFCLILFIISDILI--------------NWLEDRANF-------VNDTENPDIYCV---VNRQAMAFFLLSNILTGLTNVTIKTM 1316
            + KL  ESFISNLNGT+I+E    +S+  L   C  F  L + L+Q  +G +    K +L ++Y +++ P ILS T+FS+  Y+  ++ T A T    +      +        I  +  +   + E+   ++ +R  + ++T I ILAVD  VFPRR+AKTE +G G MDLGVG F     ++       + + +  F+ L    + +    +    R + VK  +Y EH SEYG HWNFF T+A ++ L S +   F          T +      +  + L+ F+ YGS        D K  R      AN+EG++S+ GY+ +++ G  +G  + +K    +  ++    L  I L +   F    ++ + VSRR AN  + +W  +     L L  + D+++               W+  +++F       + + +    +C+   V+R  + FFLLSNILTGL N+ + TM
Sbjct:    2 NEKLLKESFISNLNGTSISEITAGLSISSL---CFLFRGLLLVLYQEKYGPLNYSWKFHLSLDYFLLVFPLILSCTVFSDFLYIFPLSYTAACTLLFYIIYKRRTNCVKGQFICILKSFLNVHVEGETIPPVTNLRVFLNILTAISILAVDFPVFPRRYAKTETYGTGVMDLGVGGFIFANAIVSPEAKAREAVTLNRFSNLKKQLVSVWPLFILGFGRLISVKAADYHEHVSEYGVHWNFFFTLAIVRVLASFLLAVFPAKNSWVLAATFVFLYQLALETTGLKDFVFYGS--------DGKGTRV-GFFNANREGIVSVTGYVAIYVAGVQVGIYVLKKRAVLKDWISALWNLMIIVLLLFAIFHLFQIYIEPVSRRLANVTFFIWIIAQCLSFLCLIFVCDLIMVLAKYLVPGSRIPGTWVICKSSFSTKKGTKLKEGKKESSFCLIQAVSRNQLLFFLLSNILTGLVNMMVNTM 467
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Mouse
Match: Pigw (phosphatidylinositol glycan anchor biosynthesis, class W [Source:MGI Symbol;Acc:MGI:1917575])

HSP 1 Score: 135.191 bits (339), Expect = 5.293e-34
Identity = 137/498 (27.51%), Postives = 225/498 (45.18%), Query Frame = 3 
Query:   54 SSKLNHESFISNLNGTTINENYLLISLPILLNTCMS-FLSVNLFQLGFGKI-KGNLVVNYLMVIIPFILSITIFSNYLLQITLTVAA-------TXXXXXXXXXXXXXXXXYLTEIRLTLHSASNKRESWLYISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGL-----------LDQIKVGSFTKXXXXXXXXXXXXM-RTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVS-------IITKYFRFTQRIFGIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTG------CLLGAILTRKFRKSLAVFAALATIGLYISQCFLHKDI--VSRRAANFGYVLWTTSS--------------FCFCLILFIISDILINW--LEDRANFVNDTE--------NPDIYCVV---NRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIY 1358
            S K   E+F+ NL+GT++ E    +  P     C   ++  +     F        +++++++I+P ++++T+ S+++L   LTV           +   +    +     +   ++++L S  N       I+  R +  + T I ILAVD  +FPRRFAKTE++G G MD GVG F  I+G            +++ +     K       LV   M R V++K   YQEH +EYG HWNFF T+  ++LV+        + K +     I  +  + +  + L+  LLYG+        D    R    L AN+EG+IS +GY+ + + G       L G    R + K+     ++A +G +IS   +  +I  VSRR AN  + LW  +S                F   L   S +  +W  ++      N +E        N    C++   NR  + FFLLSNI TGL N+T+ T+    L ++  L IY
Sbjct:    2 SQKQLKEAFVRNLSGTSVLEVTQGLCFPAFCILCRGLWIIFSQHVCSFSNTWSTRFLMDFVVLIVPLVITLTVLSSFILLENLTVIVWGAWLLYQIYHRRTCYAKVPVQKVFANFLKISLESEYNPA-----ITCYRVINSVFTAIAILAVDFPLFPRRFAKTELYGTGAMDFGVGGF--IFGAAMVCPEVRRKSIEESRFNYLRKSLYSVWPLVFLGMGRLVIIKSIGYQEHSTEYGIHWNFFFTIIVVRLVTSLLLIIFPLNKSWIVAVSITVVYQLALDYTPLKRILLYGT--------DGSGTRV-GFLNANREGIISTLGYVTIHMAGVQTGLYVLKGRAQVRDWIKATCWVFSVA-VGFFISLHIVQVNIEAVSRRMANLAFCLWVVASSLMLLSCLLLSGIILSFAQFLIKGSLVPCSWKLIQSPTTHKNHSESLILEAEKNQPSLCLITALNRNQLFFFLLSNITTGLINLTMDTLHTGALWTLVVLSIY 482
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Mouse
Match: Pigw (phosphatidylinositol glycan anchor biosynthesis, class W [Source:MGI Symbol;Acc:MGI:1917575])

HSP 1 Score: 135.191 bits (339), Expect = 5.293e-34
Identity = 137/498 (27.51%), Postives = 225/498 (45.18%), Query Frame = 3 
Query:   54 SSKLNHESFISNLNGTTINENYLLISLPILLNTCMS-FLSVNLFQLGFGKI-KGNLVVNYLMVIIPFILSITIFSNYLLQITLTVAA-------TXXXXXXXXXXXXXXXXYLTEIRLTLHSASNKRESWLYISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGL-----------LDQIKVGSFTKXXXXXXXXXXXXM-RTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVS-------IITKYFRFTQRIFGIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTG------CLLGAILTRKFRKSLAVFAALATIGLYISQCFLHKDI--VSRRAANFGYVLWTTSS--------------FCFCLILFIISDILINW--LEDRANFVNDTE--------NPDIYCVV---NRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIY 1358
            S K   E+F+ NL+GT++ E    +  P     C   ++  +     F        +++++++I+P ++++T+ S+++L   LTV           +   +    +     +   ++++L S  N       I+  R +  + T I ILAVD  +FPRRFAKTE++G G MD GVG F  I+G            +++ +     K       LV   M R V++K   YQEH +EYG HWNFF T+  ++LV+        + K +     I  +  + +  + L+  LLYG+        D    R    L AN+EG+IS +GY+ + + G       L G    R + K+     ++A +G +IS   +  +I  VSRR AN  + LW  +S                F   L   S +  +W  ++      N +E        N    C++   NR  + FFLLSNI TGL N+T+ T+    L ++  L IY
Sbjct:    2 SQKQLKEAFVRNLSGTSVLEVTQGLCFPAFCILCRGLWIIFSQHVCSFSNTWSTRFLMDFVVLIVPLVITLTVLSSFILLENLTVIVWGAWLLYQIYHRRTCYAKVPVQKVFANFLKISLESEYNPA-----ITCYRVINSVFTAIAILAVDFPLFPRRFAKTELYGTGAMDFGVGGF--IFGAAMVCPEVRRKSIEESRFNYLRKSLYSVWPLVFLGMGRLVIIKSIGYQEHSTEYGIHWNFFFTIIVVRLVTSLLLIIFPLNKSWIVAVSITVVYQLALDYTPLKRILLYGT--------DGSGTRV-GFLNANREGIISTLGYVTIHMAGVQTGLYVLKGRAQVRDWIKATCWVFSVA-VGFFISLHIVQVNIEAVSRRMANLAFCLWVVASSLMLLSCLLLSGIILSFAQFLIKGSLVPCSWKLIQSPTTHKNHSESLILEAEKNQPSLCLITALNRNQLFFFLLSNITTGLINLTMDTLHTGALWTLVVLSIY 482
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. UniProt/SwissProt
Match: sp|Q6CK18|GWT1_KLULA (GPI-anchored wall transfer protein 1 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) OX=284590 GN=GWT1 PE=3 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 2.159e-38
Identity = 105/330 (31.82%), Postives = 166/330 (50.30%), Query Frame = 3 
Query:  435 RGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGLLDQIKVGSFTKXXXXXXXXXXXXMRTVLVKYG----------NYQEHESEYGTHWNFFLTMAALKLVSIITKYFRFTQR-IFGIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAI---LTRKFRKSLAVFAALATIGLYISQCFLHKDI--VSRRAANFGYVLWTTSSFCFCLILFIISDILINWLEDRANFVNDTENPDIYCVVNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIYTILVLI 1376
            R  +L++T+I ILAVD  +FPR++AK E +G   MDLGVG F    G++   ++ S T+LS    +        VLV  G          NYQEH +EYG HWNFF T++ L L  II  ++    R + G+I    AA I +  L+Y    L+   N  ++D     + AN+EG+ S +GY +++L G  +G++   ++R   + L    AL+     IS   LH     VSRR A+ GY   ++    F L++     +++  L  +         P  Y  VN   M  FL+SN+ TG+ N T  T++    +++  L +Y + + +
Sbjct:  123 RSTMLILTSIAILAVDFPIFPRKYAKVETWGISLMDLGVGSFVFSNGIISYKRLKSGTELSKWAKIKQSLRSTVVLVVLGFIRLFSVKAVNYQEHATEYGIHWNFFFTLSLLPLAMIIFDFYNMRLRFVIGLI----AAIIYELCLIYHPTFLDYLLNAERID----FISANREGIFSFVGYCIIYLAGQQVGSMFFPISRNPMQLLWKLVALSIFSSAISYVLLHYHPLQVSRRFASIGY---SSMVISFNLLILTFDQLIVECLTSKPRV------PKTYRAVNGNGMLVFLISNVTTGMVNFTFNTLDSPPYKAMAILTVYALFLAV 435
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. UniProt/SwissProt
Match: sp|Q873N2|GWT1_CANAL (GPI-anchored wall transfer protein 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=GWT1 PE=3 SV=2)

HSP 1 Score: 147.517 bits (371), Expect = 1.061e-37
Identity = 139/502 (27.69%), Postives = 225/502 (44.82%), Query Frame = 3 
Query:   45 LNNSSKLNHESFISNLNGTTINENYLLISLPILLNTCMSFLSVNLFQLGFGKIKGNLVVNYLMVIIPFILSITIFSNYLLQITLTVAATXXXXXXXXXXXXXXXXYLTEIRLTLHSASNKRESW--------LYISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGLLDQIKV------------GSFTKXXXXXXXXXXXXM-----RTVLVKYGNYQEHESEYGTHWNFFLTMAALKLV-SIITKYFRFTQR-IFGIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGA-------------------ILTRKFRKSLAVFAALATI-GLYISQCFLH------------KDIVSRRAANFGYVLWTTS---SFCFCLILFIISDILINWLEDRANFVNDTENPDIYCVVNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIYTI 1364
            +++S K   E F+S+L G TI E Y + S+ +      S+LS  L +   G +   L+ +Y++ ++  + SIT++SN          +     +    ++     Y  E   + H  ++ +E           +I+  R  +L++T + ILAVD  +FPRRFAK E +G   MDLGVG F    GL +  ++             S+ K      +  + I+     R V VK  +YQEHE+EYG HWNFF T+  L +V  I+        R I GI   I       Y +      L KF   ++ +R +SL+  NKEG+ S IGY+ +++ G   G+                   I  ++ +K  + F ++AT  GLY++  F H               +SRR ANF YV+W  S   +F  C  L                F+       +   +N   +  FL+SN+LTG  N++I T+E +   ++  L+ Y++
Sbjct:    1 MSSSLKQLKEQFVSDLTGGTIEEIYAVTSIAL-----SSYLSFRLLKKSLGDLA--LIYDYILNVLTILASITVYSN--------SPSYLHYFIVIPSLVIYLVNYHVEKPSSPHRQNDTKEDKSDELLPRKQFITAYRSQMLIITNLAILAVDFPIFPRRFAKVETWGTSMMDLGVGSFVFSMGLANSRQLIKNHTDNYKFSWKSYLKTIKQNFIKSVPILVLGAIRFVSVKQLDYQEHETEYGIHWNFFFTLGFLPIVLGILDPVLNLVPRFIIGIGISI------AYEVALNKTGLLKFILSSE-NRLESLITMNKEGIFSFIGYLCIFIIGQSFGSFVLTGYKTKNNLITISKIRISKKQHKKESSSFFSVATTQGLYLACIFYHLAFSLFISNLSFLQPISRRLANFPYVMWVVSYNATFLLCYDLI-------------EKFIPGNLTSTVLDSINNNGLFIFLVSNLLTGFINMSINTLETSNKMAVIILIGYSL 467
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. UniProt/SwissProt
Match: sp|Q1LZA4|PIGW_BOVIN (Phosphatidylinositol-glycan biosynthesis class W protein OS=Bos taurus OX=9913 GN=PIGW PE=2 SV=1)

HSP 1 Score: 140.969 bits (354), Expect = 2.781e-35
Identity = 136/483 (28.16%), Postives = 220/483 (45.55%), Query Frame = 3 
Query:   54 SSKLNHESFISNLNGTTINENYLLISLPILLNTCMSFLSVNLFQL--GFGKIKGNLVVNYLMVIIPFILSITIFSN-----YLLQITLTVAATXXXXXXXXXXXXXXXXYLTE--IRLTLHSASNKRESWLYISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGLLDQIKVGSFTKXXXXXXXXXXXXM----------RTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVS-------IITKYFRFTQRIFGIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAILTRKFRK-----SLAVFAALATIGLYISQCFLH--KDIVSRRAANFGYVLWTTSSFCFCLILFIISDILINWLE-----------------DRAN--------FVNDTENPDIYCVV---NRQAMAFFLLSNILTGLTNVTIKTME 1319
            S K   E+F+SN NGT++ E    + LP L   C   L +   QL       +   +V++  +I+P + ++TIFS+     YL+ I L       +    +       + + E  ++++L S     E    IS  R +    T + ILAVD  +FPRR+AKTE++G G MD GVG F     ++       +TK S    L                R V +K  +YQEH +EYG HWNFF T+ A+KL++        + + +     I  +  + +  + L+  +LYG+        D    R   LL AN+EG+IS++GY+ + + G   G  + +K         +A    L  IGL+IS   +    ++ SRR AN  + +W  +S    L   ++ DI++++ +                   AN        F    + P + C++   NR  + FFLLSN+ TGL N++I T+ 
Sbjct:    2 SQKQMKEAFVSNQNGTSVLEITEGLCLPALCILCRGLLIILSQQLCSSLHNSRTRFLVDFAFLIVPLVTTLTIFSSFVLLEYLVAIILGAGLLYEIYCRRTCYARMPFQKICEKFLKVSLES-----EHIPAISCFRVVNSAFTAVAILAVDFPLFPRRYAKTELYGTGAMDYGVGGFIFGSAMVSPEVRRKYTKGSRFCYLTKSLYSLWPLVFLGVGRLVAIKSVDYQEHLTEYGVHWNFFFTLIAVKLITSLLLLICPLNRSWVVAISIAALYQLALDFTPLKSLILYGT--------DGSGTRV-GLLNANREGIISVLGYVAVHMAGVQTGLYVLKKRSHIKDWIKVACCILLTAIGLFISLYIVQVNVEVASRRMANLAFCIWIVASCLILLSSLLLGDIILSFAKFVIKEAAVPCSWKLIQSPTANKKHLESIVFDAKRKEPTL-CLITAMNRNQLLFFLLSNVTTGLVNLSIDTLH 469
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. UniProt/SwissProt
Match: sp|Q7Z7B1|PIGW_HUMAN (Phosphatidylinositol-glycan biosynthesis class W protein OS=Homo sapiens OX=9606 GN=PIGW PE=1 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 3.533e-35
Identity = 144/497 (28.97%), Postives = 229/497 (46.08%), Query Frame = 3 
Query:   54 SSKLNHESFISNLNGTTINENYLLISLPILLNTCMSFLSV-NLFQLGFGKI-KGNLVVNYLMVIIPFILSITIFSNYLLQITLTV---AATXXXXXXXXXXXXXXXXYLTEIR----LTLHSASNKRESWLYISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGL----------LDQIKVGSFTKXXXXXXXXXXXXM-RTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVS----IITKYFRFTQRIFGIITMIMAA---SILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAIL--TRKFRKSL---AVFAALATIGLYISQCFLHKDI--VSRRAANFGYVLWTTSSFCFCLILFIISDILINWLE-----------------------DRANFVNDTE--NPDIYCVV---NRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIY 1358
            S K   E+F+SNLNGTT+ E    +  P     C  FL + + +   F    K   + +++++I+P + ++TI+++++L   L V    A     +           +L  +     ++L S  N       IS  R +    T I ILAVD  +FPRRFAKTE++G G MD GVG F     +          ++  K+  FT        LV   + R  ++K   YQEH +EYG HWNFF T+  +KL++    II    +      GI  +   A   + L+  +LYG+        D    R   LL AN+EG+IS +GY+ + + G   G  +   R   K L   A F  LA I L+IS   +  ++  VSRR AN  + +W  +S    L   ++ DI++++ +                          + V + E   P + C++   NR+ + FFLLSNI TGL N+ + T+  + L ++  + +Y
Sbjct:    2 SEKQMKEAFVSNLNGTTVLEITQGLCFPAFCILCRGFLIIFSQYLCSFSPTWKTRFLTDFVVLIVPMVATLTIWASFILLELLGVIIFGAGLLYQIYRRRTCYARLPFLKILEKFLNISLESEYNPA-----ISCFRVITSAFTAIAILAVDFPLFPRRFAKTELYGTGAMDFGVGGFVFGSAMVCLEVRRRKYMEGSKLHYFTNSLYSVWPLVFLGIGRLAIIKSIGYQEHLTEYGVHWNFFFTIIVVKLITPLLLIIFPLNKSWIIALGITVLYQLALDFTSLKRLILYGT--------DGSGTRV-GLLNANREGIISTLGYVAIHMAGVQTGLYMHKNRSHIKDLIKVACFLLLAAISLFISLYVVQVNVEAVSRRMANLAFCIWIVASSLILLSSLLLGDIILSFAKFLIKGALVPCSWKLIQSPVTNKKHSESLVPEAERMEPSL-CLITALNRKQLIFFLLSNITTGLINLMVDTLHSSTLWALFVVNLY 483
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. UniProt/SwissProt
Match: sp|Q7TSN4|PIGW_RAT (Phosphatidylinositol-glycan biosynthesis class W protein OS=Rattus norvegicus OX=10116 GN=Pigw PE=1 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 8.104e-35
Identity = 137/496 (27.62%), Postives = 225/496 (45.36%), Query Frame = 3 
Query:   54 SSKLNHESFISNLNGTTINENYLLISLPILLNTCMSFLSVNLFQLG--FGKIKGNLVVNYLMVIIPFILSITIFSNYLLQITLTVAATXXXXXXXX-------XXXXXXXXYLTEIRLTLHSASNKRESWLYISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGLL-----------DQIKVGSFTKXXXXXXXXXXXXM-RTVLVKYGNYQEHESEYGTHWNFFLTMAALKLV-SIITKYFRFTQRIFGIITMIMAASILQYFLLYGSFNLEK----FCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAILTRK-------FRKSLAVFAALATIGLYISQCFLHKDI--VSRRAANFGYVLWTTS------------SFCFCLILFIISDILI--NW---------LEDRANFVNDTENPDIYCVV---NRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIY 1358
            S K   E+F+SNL+GT++ E    +  P     C   L +    L            ++++++I+P ++++T+ S+++L   LTV      LL            +     + + ++++L S  N       I+  R +  + T I ILAVD  +FPRRFAKTE++G G MD GVG F  I+G             +  +     K       LV   M R V++K   YQEH +EYG HWNFF T+  +KL+ S++   F   +      + I+A SI   + L   F   K    +  D +  R   LL AN+EG+IS +GY+ +++ G   G  + ++        + +  VF+    +G +IS   +  ++  VSRR AN  + LW  +                    F+I   L+  +W          +  +  V   +N   +C++   NR  + FFLLSN+ TGL N+T+ T+      ++  L IY
Sbjct:    2 SQKQLKEAFVSNLSGTSVLEVTQGLCFPAFCILCRGLLIIFSQHLCSFLHTWTTQFFMDFVVLIVPLVITLTVLSSFILLENLTVILCGAWLLYQIYHRRTCYAKVPVQKVFASFLKISLESEYNPA-----ITCYRVINSVFTAIAILAVDFPLFPRRFAKTELYGTGAMDFGVGGF--IFGAAMVCPEVRRKYTEGSRFNHLRKSLYSVWPLVFLGMGRLVIIKSIGYQEHSTEYGVHWNFFFTIIVVKLITSLLLIIFPLNK------SWIVAISITVLYQLALDFTPLKGIILYGTDGRGTRV-GLLNANREGIISTLGYVAIYMAGVQTGFYVFKRRAQVRDWIKATCWVFS--VAVGFFISLNIVQVNVEAVSRRMANLAFCLWVVASSLMLLSCLLLSGIVLSFAKFLIKGALVPCSWKLIQSATTNRQSESLIVEAEKNKPSFCLITALNRNQLFFFLLSNVATGLINLTVDTLHTGAFWTLAVLSIY 481
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. TrEMBL
Match: A0A1J1IKH0 (Phosphatidylinositol-glycan biosynthesis class W protein OS=Clunio marinus OX=568069 GN=CLUMA_CG011954 PE=3 SV=1)

HSP 1 Score: 192.971 bits (489), Expect = 4.310e-52
Identity = 142/477 (29.77%), Postives = 234/477 (49.06%), Query Frame = 3 
Query:   42 LLNNSSKLNHESFISNLNGTTINENYLLISLPILLNTCMSFLSVNLFQLGFGKIKGNLVVNYLMVIIPFILSITIFSNYLLQITLTVAATXXXXXXXXXXXXXXXXYLTEIRLTLHSASNKRESWLYISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGLLDQIKVGSFTKXXXXXXXXXXXXM------RTVLVKYGNYQEHESEYGTHWNFFLTMAALKLV-SIITKYFRFTQRI--FGIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAILTRKFRKSLAVFAALATIG-LYISQCFLHKDI--------VSRRAANFGYVLWTTSSFCFCLILFIISDILI--------NWLEDRANFVNDTENPDIYC--------VVNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIYTILV 1370
            +L +  K  HESF+ N NGTT  + +L I +P       +     + ++    +    +V +    +  IL +T+FS+ + QI LT+   +  +    L          +I  ++H          +I+  R +I ++T +CILAVD + FPR+ AKTE FGFG MD+GVGLF    G++ +    +F+K     +L+    +      R ++ K  NYQEH +EYG HWNFF+T+A  K++ ++I  + + T +I    +  +I+  + LQ         L  +  D+ + R   L+ AN+EG+ S+ GY+ L+L    +G +L    R+S+     +   G L+I   F  K I        VSRR AN GY  W  +  C    LF+ ++I I         W +   N  +  + PD +          +N   +AFFL++N+ TG+ N+  +TM LN   S+  + IYT+L+
Sbjct:    1 MLPDQYKTFHESFMINNNGTTAFDTFLFI-VPSSFAIFYTITIAGILKISLNNVPIRFLVEFFTTCVSLILYLTVFSDAISQIVLTLLIITIPMAVRQLQAKFHLTPFVQIP-SVHPD--------FITVGRSVISLITAVCILAVDFQCFPRKLAKTEKFGFGLMDVGVGLFVFSNGIIVKPSASAFSKKKFQKLLVGCFPLFILGFSRFLVTKEINYQEHVTEYGVHWNFFITLAVTKIIGTVIEGFLKTTDQIKYAALSVVILHETFLQ-------LGLSNYILDDNITR-DHLISANREGIFSIPGYVALYLASIYIGTLLKSDGRESMKARDVIQKAGKLFIISLFCWKMIYVCNGMFGVSRRTANMGYNFWVLAIGCLMFSLFMFTEIYIYYTNFNRPQWQQVEPNNKSQLDVPDFHVPYVPLILQAINYNGLAFFLIANLFTGIVNICFQTMLLNTPLSVIIITIYTLLL 459
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. TrEMBL
Match: A0A2I4C528 (Phosphatidylinositol-glycan biosynthesis class W protein OS=Austrofundulus limnaeus OX=52670 GN=pigw PE=3 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 3.543e-44
Identity = 150/492 (30.49%), Postives = 240/492 (48.78%), Query Frame = 3 
Query:   72 ESFISNLNGTTINENYL--LISLPILLNTCMSFLSVNLFQLGFGKIK------GNLVVNYLMVIIPFILSITIFSNYLLQITLTVA-ATXXXXXXXXXXXXXXXXYLTEIR--LTLHSASNKRESWLYISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGL---------LDQIKVGSFTKXXXXXXXXXXXXM-RTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVS-------IITKYFRFTQRIFGIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLG--------------AILTRKFRKSLAVFAALATIGLYISQCFLHKDIVSRRAANFGYVLWTTSSFCFCLILFIISDILINWLEDRAN----------FVNDTENP--------DIYCV---VNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIY 1358
            E+F+SNLNGT++ E  L   +S    +N  + F+   L Q   G +        +L++++ ++I+P +LS TI S+ LLQ+ +++  A++ + L        +    T +   L  H+ S++     +++  R  + + T I ILAVD  VFPRR+AKTE +G G MD GVG F     L         +   K+  FTK       L+     R V VK   Y EH +EYG HWNFF T+A +++++            + F   I G+   I+  S L+ F+L          ND   DR K  + ANKEG+ SL+GY+ ++LTG  +G               +L+  F  SL +FAAL         C    + VSRR+ANF + LW+ +   F +  F ++D+L+ +L+  +           +  D +          +  C+   V+R  + FFLLSN++TGLTN  + T+  + L S+  L++Y
Sbjct:    8 EAFVSNLNGTSLMEVALGSFLSPVCFMNRGLIFI---LCQQXKGSLPLPFPMTSHLLLDFCVLILPLVLSCTILSSVLLQVAVSLTFASASMFLYIYRSSFHAQHSQTSVSGFLQSHTQSDQVP---FVTLFRVFVNVKTAISILAVDFAVFPRRYAKTETYGTGVMDFGVGGFVFANALVCPEARRKNISGSKMSYFTKQVVSVWPLLALGAGRLVSVKMTGYHEHVTEYGVHWNFFFTLAVVRILASGLLTVLPARHLWVFALLISGLYQFILETSELKAFILR---------ND---DREKGFVHANKEGIFSLVGYVSIYLTGVQIGLYVMQPRSRVGEWLKVLSCFFLGSLVLFAALCA-------CQTLVEPVSRRSANFPFFLWSLAQSLFFMSCFGLADMLLLFLKRASGCHVVPSSLNVYDKDADKSLGRKRGEMEKLCIVQAVSRNQLLFFLLSNVMTGLTNSLVDTLSCSSLYSVCVLLLY 474
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. TrEMBL
Match: A0A3Q2YS44 (Phosphatidylinositol-glycan biosynthesis class W protein OS=Hippocampus comes OX=109280 PE=3 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 1.172e-43
Identity = 152/482 (31.54%), Postives = 241/482 (50.00%), Query Frame = 3 
Query:   54 SSKLNHESFISNLNGTTINENYL--LISLPILLNTCMSFL----SVNLFQLGFGKIKGNLVVNYLMVIIPFILSITIFSNYLLQITLTVAATXXXXXXXXXXXXXXXXYLTEIRLTLHSASNKRESWLYISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGLLDQIKVGSFT----------KXXXXXXXXXXXXMRTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVSIITKYFRFTQRIFGIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAILTRK--------------FRKSLAVFAALATIGLYISQCFLHKDIVSRRAANFGYVLWTTSS-----FCFCLILFIISDILI---------NWLEDRANFVNDTENPDIYCVV---NRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIY 1358
            S K   E+F+SNLNGT++ E  +   ++   LLN  +  +    +     L F +I  +L ++++M+ +P +LS T+ SN+LLQ+ L+ A     +L +    +  +   T +  T+H          +++  R ++ + T I ILAVD +VFPRR+AKTE FG G MD   G F I   L+     G             +L S+  L+VLG  R V VK  NYQEH +EYG HWNFF T+A +++++ +       +  +  I  ++ + + Q+ L   +  L+ F   N  DR K  L ANKEG+ SL GY+ +++ G  +G  + +               F  +LAVFAAL T  +++       + +SRR AN  + LWT +       CF L+  II    I         +W +  +      ++ + +C+V   NR  + +FLLSNILTGLTN  I T+  + + S   L+ Y
Sbjct:    2 SQKELKEAFVSNLNGTSLQEVVIGSFLTPLCLLNRALVLILYHQAKGNLPLPFPRI-SHLFLDFIMMALPLVLSCTVLSNHLLQVVLSFAFVLLCVLFHIYYSNHQSSLSTFLNSTVHFKQVP-----FVTFFRVMLNVQTAISILAVDFEVFPRRYAKTESFGTGVMDFCAGGFIIGNALVCPEARGKNVYRSKMKHVGKQLLSVWPLVVLGAARLVFVKMSNYQEHVTEYGVHWNFFFTLALVRVLTSMVLVVVPAKHSW--ILALVISCVYQFAL--DTTGLKAFIMHND-DREKDFLHANKEGIFSLAGYVAIYMAGVQVGCYVMQPRSHVRDWLKAVFNLFMGTLAVFAALITCQIFV-------EPMSRRLANLPFCLWTVAQSLLFVSCFGLVELIILCFKITSCCHFVPSSW-DSYSGSHKKKDDLEGFCLVNAINRNQLLYFLLSNILTGLTNTLINTLNCSDVFSFCVLLSY 464
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. TrEMBL
Match: A0A0E9X650 (Phosphatidylinositol-glycan biosynthesis class W protein OS=Anguilla anguilla OX=7936 PE=3 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 1.690e-43
Identity = 153/494 (30.97%), Postives = 239/494 (48.38%), Query Frame = 3 
Query:   54 SSKLNHESFISNLNGTTINENYLLISL-PILLNTCM--SFLSVNLFQLGFGKI----KGNLVVNYLMVIIPFILSITIFSNYLLQITLTVAA--TXXXXXXXXXXXXXXXXYLTEIRLTLHSASNKRESWLYISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGLLDQ---------IKVGSFTKXXXXXXXXXXXXM-RTVLVKYGNYQEHESEYGTHWNFFLTMAALKLV-SIITKYFRFTQRIF------GIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLG------AILTRKFRKSLAVFAALATIG----LYISQCFLHKDIVSRRAANFGYVLWTTSSFCFCLILFIISDILINWLEDRAN--FVNDTENP------------------DIYCV---VNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIY 1358
            S K   E+F+SNLNGT++ E    +SL  +L   C+    L + L+ LG G +    K +L++++  +I+P +LS TI S++L  + L++AA  TS L          S+ +  ++       + +     ++S  R L+ + T I ILAVD  VFPRR+AK E +G G MD GVG +     L+            K+    K       L L  M R + +K   Y EH +EYG HWNFF T+A +++V S+I   F   +  F      G+    +  + L+ F+++ S            DR    L ANKEG+ S+IGYI ++L G  +G        L + + K++     L + G    LY+ Q F+    VSRR AN  + +WT +   F L  F I+DI++ + +  AN   ++ + NP                  + +C+   VNR  + FFLL+NI TG TN  + T+  N   S+  L+ Y
Sbjct:    2 SQKELKEAFVSNLNGTSLGE----VSLGSVLAPLCLMSRGLVLVLYYLGKGVLPFTWKVHLLMDFTFLILPLVLSCTILSDFLQVVILSLAAINTSLLFSVYYRKKHSSSVHSRDVFKNFLQINLETNRIPFVSLFRVLVNVKTAISILAVDFSVFPRRYAKAETYGTGVMDFGVGAYIFANALVCPEARRKEVYVSKISYVAKQLLSVWPLALLGMARLISLKVTGYHEHVTEYGVHWNFFFTLAVVRVVASVILALFPVNRSWFCALIIGGLYQTTLEMTELKSFIIHNS------------DRMGGFLSANKEGVFSVIGYIAIYLAGVQVGLYVMQARTLVKDWIKAVCNL-LLGSFGLFAFLYVCQTFIEP--VSRRMANLSFCVWTVAQSLFFLSCFGIADIVLVFSKSLANCTLISSSWNPIKNAKSETLSAEKIRDKMEGFCLIQAVNRNQLLFFLLANIFTGFTNAVVDTVSCNNFHSVCVLLCY 476
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. TrEMBL
Match: A0A069DTQ1 (Phosphatidylinositol-glycan biosynthesis class W protein (Fragment) OS=Panstrongylus megistus OX=65343 PE=2 SV=1)

HSP 1 Score: 169.088 bits (427), Expect = 2.445e-43
Identity = 152/467 (32.55%), Postives = 232/467 (49.68%), Query Frame = 3 
Query:   69 HESFISNLNGTTINENYLLISLPILLNTCMSFLSVNLFQLGFGKIKGNLVVNYLMVIIPFI----LSITIFSNYL-LQITLTVAATXXXXXXXXXXXXXXXXYLTEIRLTLHSASNKR-ESWLYISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGLLDQIKVGSFT---KXXXXXXXXXXXXMRTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVSIITKYFRFTQRIFGIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAILT-------RKFRKSLAVFAALATIGLYISQCFLHKDIVSRRAANFGYVLWTTSSFCFCLILFIISDILINWLEDRANFVNDTEN-----PDIYCVVNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIYTILV--LITTPHINGI 1400
            HESF+ N NGT+I EN ++IS   +L   +  +   LFQ G    K    V++++  I FI    L+ T+ S+Y+ + +TL + A           +     Y         +   KR E   Y +  R +I +MT+ICILAVD  +FPRRFAKTE +G+G MD GVG + +  G++ +     +     L S   +++LGI+R   ++  +YQ+H SEYG HWNFF T+A +K++S +  Y +F  ++ G+  +I   S     LLY  F  EK+  ++      +   ANKEG++SL GYI L+L    +G  L           R  +  F       +         D VSRR AN  Y+ W  +   + + L I ++I +     R  F    E+     P I   VN  ++ +FLLSNILTG+ N+  +T   ++L SI  LV+Y      +I   H+N I
Sbjct:    5 HESFMQNHNGTSIWENIMVISPGPVLVYFVCLIQFYLFQ-GKPTNKREHAVSFIVEFICFIYFLILNFTLLSDYIYIHVTLLLVA----------YIFFLYHYSKGASKGFFNVPRKRIEPRPYFTYFRSIISIMTSICILAVDFNIFPRRFAKTETYGYGLMDTGVGFYIVANGIVAKQNDQRYVLIKSLWSSIPIILLGIIRYASMETLDYQKHISEYGVHWNFFFTLAFVKIISSLLIY-KFPGKVVGMALLI---SFSHQMLLY--FVTEKWIIEDSF--RSNFFSANKEGIVSLPGYISLYLFSVAIGKFLNMSQIRLIDDVRHGINTFIWTLIFLILTLLFHFMFD-VSRRLANLTYITWMLTMSLYGISLSIFTEIAL-----RVTFQMKKEDINLLTPSILNAVNYNSLVYFLLSNILTGIVNLIFQTYFADQLLSIAILVLYKFCACGIIYVIHLNKI 446
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Cavefish
Match: pigw (phosphatidylinositol glycan anchor biosynthesis class W [Source:NCBI gene;Acc:103043659])

HSP 1 Score: 162.155 bits (409), Expect = 7.062e-44
Identity = 153/498 (30.72%), Postives = 252/498 (50.60%), Query Frame = 3 
Query:   75 SFISNLNGTTINENYLLISLPILLNTCMSFLSVNLFQLGFGKIKG--------NLVVNYLMVIIPFILSITIFSNYLLQITLTVAATXXXXXXXXXXXXXXXXYLTEIRLTLHSASNKR-ESWL--YISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGLL------DQIKVGSF----TKXXXXXXXXXXXXMRTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVS-------IITKYFRFTQRIFGIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAIL--TRKFRKSLAVFAA---LATIGLYIS--QCFLHKDIVSRRAANFGYVLWTTSSFCFCLILFIISDILINWLEDRANF--VNDTENP--------------DIYCV---VNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIYTIL--VLITTPHINGI 1400
            +F+SNLNGTT+ E    +SL   L   +  LS  LF + F   +G        +LV+++  +I+P +LS T+ S+ L  + + +A     +L       K++        +LHS  N   E+ L  +++  R L+ + T I ILAVD  VFPRR+AKTE +G G MD GVG + +   L+       +I+   F     +L S+  L++LG++R   VK   Y EH +EYG HWNFF T+A +++V+        I+  +     I G+   ++  + L+ F+++ S            +RT + L AN+EG+ S++GY+ +++ G  +G  L  TR   K   +      L + GL++    C  + + VSRR AN  + +WT +   F L     +D+++ + +  + F  V+ + NP              D  C+   VNR  + FFLL+N++TGLTNV + T++ N   S+  L  Y ++  + I   HIN I
Sbjct:    9 AFVSNLNGTTVAE----VSLGSFLAP-LCILSRGLFLIVFHLCRGVLPLAKGAHLVLDFTALIMPMVLSCTVLSDILHWVIVGLAVADLFVLYFLYRNKKAHTG-GSFGGSLHSFMNSHMETNLVPFVTAFRVLVNVKTAISILAVDFSVFPRRYAKTETYGTGVMDFGVGAYVMANALVCPEARGKKIQGSKFKYVVKQLLSVWPLVLLGLVRLASVKSTGYHEHVTEYGVHWNFFFTLAIVRVVASALLSVFPISSSWLIALLISGVYQFLLETTDLKSFIIHSS------------ERT-TFLQANREGIFSVVGYVAIYMAGVQVGLYLMRTRTLVKDWILVVRNLLLGSFGLWLVLYACQTYTEPVSRRMANLSFCIWTVAQSLFFLACLSTADVVLLFAKTMSKFSLVSTSWNPAGSSQTEGKLRKKMDELCLIQSVNRNQLLFFLLANLMTGLTNVLVDTLKSNDFFSVCVLQSYMLMNSLCIFILHINNI 487
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005068.1 (pep scaffold:Pmarinus_7.0:GL484072:6792:9750:1 gene:ENSPMAG00000004602.1 transcript:ENSPMAT00000005068.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 84.3445 bits (207), Expect = 3.529e-19
Identity = 58/185 (31.35%), Postives = 95/185 (51.35%), Query Frame = 3 
Query:   72 ESFISNLNGTTINENYLLISLPILLNTCMSFLSVNLFQLG---FGKIKGNLVVNYLMVIIPFILSITIFSNYLLQITLTVAA--------TXXXXXXXXXXXXXXXXYLTEIRLTLHSASNKRESWLY--ISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGLLDQ 587
            E+F+SNLNGT ++E  +L+++    + C   L   LF  G    G  +   V ++L++++P+ LS T+ + ++    +T  A        T     +           +T  +L L+ + N +E      ++Q R    ++T +CILAVD + FPRRFAKTE+ G G MDLGVG F +   L+  
Sbjct:    8 EAFVSNLNGTNVSEIAILLTILPAGDFCRGLLG--LFWAGSASAGAHRVAFVRDFLLLVLPYTLSTTVLAPHITLEKITKFAWNNAKELHTRMTSHACISRKKWHRVMVTFAKLMLNVSENPKEEGHVRCLTQFRAYTNLLTIVCILAVDFRAFPRRFAKTEIHGTGLMDLGVGTFVLSNALVSP 190
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Yeast
Match: GWT1 (Protein involved in the inositol acylation of GlcN-PI; the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) forms glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors [Source:SGD;Acc:S000003627])

HSP 1 Score: 123.25 bits (308), Expect = 5.178e-31
Identity = 148/496 (29.84%), Postives = 222/496 (44.76%), Query Frame = 3 
Query:   60 KLNHESFISNLNGTTINENYLLISLPILLNTCMSFL-SVNLFQLGFGKIKGNLVVNYLMVIIPFILSITIFSN--YLLQ--ITLTVAATXXXXXXXXXXXXXXXXYLTEIRLTLHSASNKRESWLYISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYG------LLDQIKVGS--------FTKXXXXXXXXXXXXMRTVLVKYGNYQEHESEYGTHWNFFLTMAALKLV-SIITKYFRFTQRIFGIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLV---------ANKEGLISLIGYIVLWLTGCLLGAIL--------------------------TRKFRKSLAVFAAL---ATIGLYISQCFL--HKDIVSRRAANFGYVLWTTSSFCFCLILFIISDILINWLEDRANFVNDTENPDIY-CV--VNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIY 1358
            K   E F++ LNG +I E   + S+ ++     + L + NL   G   ++   ++++ +  +  +LSITI+++  YLL   I L            S     +  Y  +  +T      K+    YI+  RG +L++T I ILAVD  +FPRRFAK E +G   MDLGVG F    G      LL  + + S        F  L S G LL LG++R   VK   YQEH +EYG HWNFF+T++ L LV + I    R   R       I  + I ++ LL             K DRT + L+         AN+EG+ S +GY  ++L G   G  L                          T  +  S+   + L   +TI L ISQ     H   VSRR AN  Y LW  +     L  + ++D +         F N +E   +  C+  +N   +  FLL+N+ TGL N+++ T++ + L+S   L+ Y
Sbjct:    5 KQRKEDFVTGLNGGSITEINAVTSIALVTYISWNLLKNSNLMPPGISSVQ--YIIDFALNWVALLLSITIYASEPYLLNTLILLPCLLAFIYGKFTSSSKPSNPIYNKKKMITQRFQLEKKP---YITAYRGGMLILTAIAILAVDFPIFPRRFAKVETWGTSLMDLGVGSFVFSNGIVSSRALLKNLSLKSKPSFLKNAFNALKSGGTLLFLGLLRLFFVKNLEYQEHVTEYGVHWNFFITLSLLPLVLTFIDPVTRMVPR---CSIAIFISCIYEWLLL-------------KDDRTLNFLILADRNCFFSANREGIFSFLGYCSIFLWGQNTGFYLLGNKPTLNNLYKPSTQDVVAASKKSSTWDYWTSVTPLSGLCIWSTIFLVISQLVFQYHPYSVSRRFANLPYTLWVITYNLLFLTGYCLTDKI---------FGNSSEYYKVAECLESINSNGLFLFLLANVSTGLVNMSMVTIDSSPLKSFLVLLAY 470
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Nematostella
Match: EDO39058 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SB56])

HSP 1 Score: 166.392 bits (420), Expect = 4.203e-46
Identity = 152/450 (33.78%), Postives = 227/450 (50.44%), Query Frame = 3 
Query:   69 HESFISNLNGTTINENYLLIS---LPILLNTCMSFLSVNLFQ-LGFG---KIKGNLVVNYLMVIIPFILSITIFSNYLLQITLTVAATXXXXXXXXXXXXXXXXYLTEIRLTLHSASNKRESWLYISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAI-----------IYGLLDQIKVGSFTKXXXXXXXXXXXXMRTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVSIITKYFRFTQRIFGIITMIMAASILQYFL---------LYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAILTRK---FRKSLAVFAALATIG--LYISQCFLHKDI--VSRRAANFGYVLWTTSSFCFCLILFIISDILINWLEDRANFVNDTENPDIYCVVNRQAMAFFLLSNILTGLTNVTIKTM 1316
             + F+SNLNGTT++E  +++S   L +LL T   FL V L + L      K    ++++Y  ++ P +++ TI S ++  I      T  L L+ +LI++ SN  +  +   L S  + R+   +I   R  +L+ T I ILAVD K+FPRRFAK E +G G MD+GVG F +            +  L  I   + + + S   LLVLG+MR V VK  +YQEH SEYG HWNFFLT+  ++++S          +++G+   ++A  + QYFL         LYGS         N       LL AN+EGL+S IGY+ L+     LG  L  K   F   + VF  L ++    +I       +I  VSRR AN  +VLW   S  +C  L   S   +     R   + + E   +   +NR  + +FLL+NILTG  N  ++T+
Sbjct:   13 QQLFVSNLNGTTLSEIAVVVSSAPLAVLLRT---FLGVTLLRSLSIDSPFKSSAIILLDYATLVFPVLMNFTIMSEHV-GIYFITMVTVMLSLTVTLIITNSNHAVPTMSQILQSRMSGRQP--FICNFRAYVLVATAISILAVDFKIFPRRFAKAETYGSGLMDVGVGAFVVSNAIVSREARQAHKPLGGIVKNTVSSIRSGLPLLVLGMMRCVAVKSTDYQEHVSEYGVHWNFFLTLFVVRVLSSFIVCIIPGSKLYGLAGFLLAI-LYQYFLSNLGLRQIVLYGS---------NNDGSRNGLLDANREGLVSNIGYLALYFLALQLGKFLFEKRDTFASWMKVFFVLVSLDALFWIGTLLCSHNISPVSRRLANLSFVLWQVLSLYYCCYLHHTSHSSL-----RRKSIKECECQCLIAAINRNQLVYFLLANILTGAVNFLVQTL 441
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Medaka
Match: pigw (phosphatidylinositol glycan anchor biosynthesis class W [Source:NCBI gene;Acc:101167143])

HSP 1 Score: 145.591 bits (366), Expect = 5.837e-38
Identity = 142/513 (27.68%), Postives = 236/513 (46.00%), Query Frame = 3 
Query:   54 SSKLNHESFISNLNGTTINENYLLISLPILLNTCMSFLSVNLFQLGFGKIK-----GNLVVNYLMVIIPFILSITIFSNYLLQITLTVAATXXXXXXXXXXXXXXXXYLTEIRLTLHSASNKRESWL-----------YISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGL---------LDQIKVGSFTKXXXXXXXXXXXXMRTVL-VKYGNYQEHESEYGTHWNFFLTMAALKLV--SIIT-----KYFRFTQRIFGIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGA-ILTRKF--RKSLAVFAALATIGLYIS----QCFLHKDIVSRRAANFGYVLWTTSS---FCFCL---------------ILFIISDI-LINWLEDRANFVNDTENPDIYCV---VNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIYTIL--VLITTPHINGI 1400
            + K   E+F++NLNGT + E  L   L  L      FL +  +Q     +       ++ ++  +++ P +LS T+ S+ L Q+ L++A               S      IRL+   ++ K  S             +++  R  + + T I ILAVD  VFPRR+AKTE +G G MD GVG F     L         +   ++G  T+       L+   +  +L VK  +YQEH +EYG HWNFF T+A +++V  +++T     + + F     G+    +  S L+ FL++ +            DR K  L ANKEG+ S++G + ++L G   G  ++  +F  ++ L +   L     ++      C    + VSRR AN  +V+W+      F  CL                 F+ S + L    E+R     DT   +  C+   V+R  + FFLL+N++TGLTN+ + T+  +   S++ L++Y  +  +L+   HI GI
Sbjct:    2 AQKEFKEAFVANLNGTGLEEVVLGSFLSPLCFIIRGFLLILCYQTRGAVVLPLPPLSHMFLDVFVLVFPLVLSCTVLSSVLHQVILSLA-------------FISAGLFCWIRLSTDRSAQKSASSFLQTPVQCDQVPFVTLFRVFVNVKTAISILAVDFPVFPRRYAKTETYGTGVMDFGVGAFVFANALVCPEARRKNISGSRMGHVTQQIVSVWPLLALGLARLLSVKLADYQEHVTEYGVHWNFFFTLAMVRIVASALLTILPSRQLWVFALLFSGLYQFTLETSELKAFLIHNN------------DREKDFLHANKEGIFSIVGNVAIYLIGVQFGLYVMQPRFHVKEWLKMLLNLLFCSFFLYCVLFACQTFVEPVSRRLANLPFVIWSVGQSLLFMSCLGVADMVLLFSKKSSGCPFVPSSLSLCRKKEER----TDTTGLERLCLIKAVHRNQLLFFLLANVMTGLTNMVVDTLGSDGFFSVSVLLLYMFVNCILVHVLHILGI 485
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Planmine SMEST
Match: SMESG000059050.1 (SMESG000059050.1)

HSP 1 Score: 845.884 bits (2184), Expect = 0.000e+0
Identity = 452/455 (99.34%), Postives = 454/455 (99.78%), Query Frame = 3 
Query:   39 MLLNNSSKLNHESFISNLNGTTINENYLLISLPILLNTCMSFLSVNLFQLGFGKIKGNLVVNYLMVIIPFILSITIFSNYLLQITLTVAATXXXXXXXXXXXXXXXXYLTEIRLTLHSASNKRESWLYISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGLLDQIKVGSFTKXXXXXXXXXXXXMRTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVSIITKYFRFTQRIFGIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAILTRKFRKSLAVFAALATIGLYISQCFLHKDIVSRRAANFGYVLWTTSSFCFCLILFIISDILINWLEDRANFVNDTENPDIYCVVNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIYTILVLITTPHINGIL 1403
            MLLNNSSKLNHESFISNLNGTTINENYLLISLPILLNTCMSFLSVNLFQLGFGKIKGNLVVNYLMVIIPFILSITIFSNYLLQITLTVAATSFLLLSNSLILSKSNKYLTEIRLTLHSASNKRESWLY+SQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGLLDQIKVGSFTKLSSIGILLVLGIMRTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVSIITKYFRFTQRIFGIITMIMA SILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAILTRKFRKSLAVFAALATIGLYISQCFLHKDIVSRRAANFGYVLWTTSSFCFCL+LFIISDILINWLEDRANFVNDTENPDIYCVVNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIYTILVLITTPHINGIL
Sbjct:    1 MLLNNSSKLNHESFISNLNGTTINENYLLISLPILLNTCMSFLSVNLFQLGFGKIKGNLVVNYLMVIIPFILSITIFSNYLLQITLTVAATSFLLLSNSLILSKSNKYLTEIRLTLHSASNKRESWLYMSQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGLLDQIKVGSFTKLSSIGILLVLGIMRTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVSIITKYFRFTQRIFGIITMIMAVSILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAILTRKFRKSLAVFAALATIGLYISQCFLHKDIVSRRAANFGYVLWTTSSFCFCLMLFIISDILINWLEDRANFVNDTENPDIYCVVNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIYTILVLITTPHINGIL 455
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Planmine SMEST
Match: SMESG000059050.1 (SMESG000059050.1)

HSP 1 Score: 770 bits (1987), Expect = 0.000e+0
Identity = 413/416 (99.28%), Postives = 415/416 (99.76%), Query Frame = 3 
Query:  156 MSFLSVNLFQLGFGKIKGNLVVNYLMVIIPFILSITIFSNYLLQITLTVAATXXXXXXXXXXXXXXXXYLTEIRLTLHSASNKRESWLYISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGLLDQIKVGSFTKXXXXXXXXXXXXMRTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVSIITKYFRFTQRIFGIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAILTRKFRKSLAVFAALATIGLYISQCFLHKDIVSRRAANFGYVLWTTSSFCFCLILFIISDILINWLEDRANFVNDTENPDIYCVVNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIYTILVLITTPHINGIL 1403
            MSFLSVNLFQLGFGKIKGNLVVNYLMVIIPFILSITIFSNYLLQITLTVAATSFLLLSNSLILSKSNKYLTEIRLTLHSASNKRESWLY+SQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGLLDQIKVGSFTKLSSIGILLVLGIMRTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVSIITKYFRFTQRIFGIITMIMA SILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAILTRKFRKSLAVFAALATIGLYISQCFLHKDIVSRRAANFGYVLWTTSSFCFCL+LFIISDILINWLEDRANFVNDTENPDIYCVVNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIYTILVLITTPHINGIL
Sbjct:    1 MSFLSVNLFQLGFGKIKGNLVVNYLMVIIPFILSITIFSNYLLQITLTVAATSFLLLSNSLILSKSNKYLTEIRLTLHSASNKRESWLYMSQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAIIYGLLDQIKVGSFTKLSSIGILLVLGIMRTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVSIITKYFRFTQRIFGIITMIMAVSILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAILTRKFRKSLAVFAALATIGLYISQCFLHKDIVSRRAANFGYVLWTTSSFCFCLMLFIISDILINWLEDRANFVNDTENPDIYCVVNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIYTILVLITTPHINGIL 416
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Planmine SMEST
Match: SMESG000069660.1 (SMESG000069660.1)

HSP 1 Score: 79.337 bits (194), Expect = 1.287e-15
Identity = 89/350 (25.43%), Postives = 157/350 (44.86%), Query Frame = 3 
Query:  423 ISQMRGLILMMTTICILAVDMKVFPRRFAKTEMFGFGPMDLGVGLFAI---IYGLLDQIKVGSFTKXXXXXXXXXXXX--------MRTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVSIITKYFRFTQRI----FGIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAILTRKFR-----KSLAVFAALATIGLYISQCFLHKDIV---SRRAANFGYVLWTTSSFCFCLILFIISDILINWLEDRANFVNDTENPDIYCVVNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIYTILVLITTPHINGIL 1403
            +S  RG++ +MT ICI A D+  F     K+  FG G MD+G+G F +     G  ++   G       I + ++           +R  ++K         EYG   N F  ++ +KL   I +   F +++      I+ MI+    L+   +Y   N   +  D ++D  K++ VAN E   SL+G++ L+ +G  +G I   +       KS +     + I  ++ +  + ++I+   SRR  N  YVLW  S F F L L   + I++ + E  +NF       +    ++R    +F++ NI+ G+  +      L K  S   L +Y  + +I +  IN +L
Sbjct:  100 LSMHRGIVYIMTCICIFARDLPEFRSNLGKSSYFGSGFMDVGIGFFVLHMGTKGYGNKWSTGEDNGKRKILLRIMAISGVLFLMGIIRYAILKITKLSYSPIEYGRDMNVFCVLSFVKLAGSILE-IAFHKKVEPWKLSILMMIIYEIRLKS-NIYSESNFNSY--DFRLD--KNVYVANIEFFNSLMGHLALFFSGRQIGIIFFERISSTNKLKSSSKMLITSIIIFFVKEFLIFENILEEESRRMCNLSYVLWMISIFLFGLFL---TRIMMVYFE--SNFEGKYHYTE---RIDRMGFIYFIVGNIIVGVYRLCGNERSLPKWSSSIILYVYCSINVILSTFINKLL 435
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Planmine SMEST
Match: SMESG000069660.1 (SMESG000069660.1)

HSP 1 Score: 56.9954 bits (136), Expect = 1.557e-8
Identity = 78/321 (24.30%), Postives = 141/321 (43.93%), Query Frame = 3 
Query:  510 KTEMFGFGPMDLGVGLFAI---IYGLLDQIKVGSFTKXXXXXXXXXXXX--------MRTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVSIITKYFRFTQRI----FGIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAILTRKFR-----KSLAVFAALATIGLYISQCFLHKDIV---SRRAANFGYVLWTTSSFCFCLILFIISDILINWLEDRANFVNDTENPDIYCVVNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIYTILVLITTPHINGIL 1403
            K+  FG G MD+G+G F +     G  ++   G       I + ++           +R  ++K         EYG   N F  ++ +KL   I +   F +++      I+ MI+    L+   +Y   N   +  D ++D  K++ VAN E   SL+G++ L+ +G  +G I   +       KS +     + I  ++ +  + ++I+   SRR  N  YVLW  S F F L L   + I++ + E  +NF       +    ++R    +F++ NI+ G+  +      L K  S   L +Y  + +I +  IN +L
Sbjct:  113 KSSYFGSGFMDVGIGFFVLHMGTKGYGNKWSTGEDNGKRKILLRIMAISGVLFLMGIIRYAILKITKLSYSPIEYGRDMNVFCVLSFVKLAGSILE-IAFHKKVEPWKLSILMMIIYEIRLKS-NIYSESNFNSY--DFRLD--KNVYVANIEFFNSLMGHLALFFSGRQIGIIFFERISSTNKLKSSSKMLITSIIIFFVKEFLIFENILEEESRRMCNLSYVLWMISIFLFGLFL---TRIMMVYFE--SNFEGKYHYTE---RIDRMGFIYFIVGNIIVGVYRLCGNERSLPKWSSSIILYVYCSINVILSTFINKLL 419
[Close]
BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Planmine SMEST
Match: SMESG000069660.1 (SMESG000069660.1)

HSP 1 Score: 56.6102 bits (135), Expect = 1.957e-8
Identity = 78/321 (24.30%), Postives = 141/321 (43.93%), Query Frame = 3 
Query:  510 KTEMFGFGPMDLGVGLFAI---IYGLLDQIKVGSFTKXXXXXXXXXXXX--------MRTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVSIITKYFRFTQRI----FGIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISLIGYIVLWLTGCLLGAILTRKFR-----KSLAVFAALATIGLYISQCFLHKDIV---SRRAANFGYVLWTTSSFCFCLILFIISDILINWLEDRANFVNDTENPDIYCVVNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIYTILVLITTPHINGIL 1403
            K+  FG G MD+G+G F +     G  ++   G       I + ++           +R  ++K         EYG   N F  ++ +KL   I +   F +++      I+ MI+    L+   +Y   N   +  D ++D  K++ VAN E   SL+G++ L+ +G  +G I   +       KS +     + I  ++ +  + ++I+   SRR  N  YVLW  S F F L L   + I++ + E  +NF       +    ++R    +F++ NI+ G+  +      L K  S   L +Y  + +I +  IN +L
Sbjct:  109 KSSYFGSGFMDVGIGFFVLHMGTKGYGNKWSTGEDNGKRKILLRIMAISGVLFLMGIIRYAILKITKLSYSPIEYGRDMNVFCVLSFVKLAGSILE-IAFHKKVEPWKLSILMMIIYEIRLKS-NIYSESNFNSY--DFRLD--KNVYVANIEFFNSLMGHLALFFSGRQIGIIFFERISSTNKLKSSSKMLITSIIIFFVKEFLIFENILEEESRRMCNLSYVLWMISIFLFGLFL---TRIMMVYFE--SNFEGKYHYTE---RIDRMGFIYFIVGNIIVGVYRLCGNERSLPKWSSSIILYVYCSINVILSTFINKLL 415
The following BLAST results are available for this feature:
BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PIGW7.356e-3628.97phosphatidylinositol glycan anchor biosynthesis cl... [more]
PIGW7.356e-3628.97phosphatidylinositol glycan anchor biosynthesis cl... [more]
PIGW7.356e-3628.97phosphatidylinositol glycan anchor biosynthesis cl... [more]
PIGW7.356e-3628.97phosphatidylinositol glycan anchor biosynthesis cl... [more]
PIGW4.619e-2331.37phosphatidylinositol glycan anchor biosynthesis cl... [more]
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
hpo-201.406e-3027.15Phosphatidylinositol-glycan biosynthesis class W p... [more]
hpo-205.138e-926.85Phosphatidylinositol-glycan biosynthesis class W p... [more]
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3
Match NameE-valueIdentityDescription
PIG-Wb1.313e-1828.53gene:FBgn0035265 transcript:FBtr0334076[more]
PIG-Wb1.313e-1828.53gene:FBgn0035265 transcript:FBtr0072796[more]
PIG-Wa2.016e-1525.08gene:FBgn0031422 transcript:FBtr0077768[more]
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pigw1.562e-4129.51phosphatidylinositol glycan anchor biosynthesis, c... [more]
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ndrg19.979e-4128.87N-myc downstream regulated 1 [Source:Xenbase;Acc:X... [more]
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Pigw5.293e-3427.51phosphatidylinositol glycan anchor biosynthesis, c... [more]
Pigw5.293e-3427.51phosphatidylinositol glycan anchor biosynthesis, c... [more]
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6CK18|GWT1_KLULA2.159e-3831.82GPI-anchored wall transfer protein 1 OS=Kluyveromy... [more]
sp|Q873N2|GWT1_CANAL1.061e-3727.69GPI-anchored wall transfer protein 1 OS=Candida al... [more]
sp|Q1LZA4|PIGW_BOVIN2.781e-3528.16Phosphatidylinositol-glycan biosynthesis class W p... [more]
sp|Q7Z7B1|PIGW_HUMAN3.533e-3528.97Phosphatidylinositol-glycan biosynthesis class W p... [more]
sp|Q7TSN4|PIGW_RAT8.104e-3527.62Phosphatidylinositol-glycan biosynthesis class W p... [more]
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1J1IKH04.310e-5229.77Phosphatidylinositol-glycan biosynthesis class W p... [more]
A0A2I4C5283.543e-4430.49Phosphatidylinositol-glycan biosynthesis class W p... [more]
A0A3Q2YS441.172e-4331.54Phosphatidylinositol-glycan biosynthesis class W p... [more]
A0A0E9X6501.690e-4330.97Phosphatidylinositol-glycan biosynthesis class W p... [more]
A0A069DTQ12.445e-4332.55Phosphatidylinositol-glycan biosynthesis class W p... [more]
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pigw7.062e-4430.72phosphatidylinositol glycan anchor biosynthesis cl... [more]
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000005068.13.529e-1931.35pep scaffold:Pmarinus_7.0:GL484072:6792:9750:1 gen... [more]
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
GWT15.178e-3129.84Protein involved in the inositol acylation of GlcN... [more]
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO390584.203e-4633.78Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
pigw5.837e-3827.68phosphatidylinositol glycan anchor biosynthesis cl... [more]
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BLAST of Phosphatidylinositol-glycan biosynthesis class W protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000059050.10.000e+099.34SMESG000059050.1[more]
SMESG000059050.10.000e+099.28SMESG000059050.1[more]
SMESG000069660.11.287e-1525.43SMESG000069660.1[more]
SMESG000069660.11.557e-824.30SMESG000069660.1[more]
SMESG000069660.11.957e-824.30SMESG000069660.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024079 ID=SMED30024079|Name=Phosphatidylinositol-glycan biosynthesis class W protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1455bp
TATAACTCACTATAACTTATTTTAAGTAACTATTTCAAATGTTATTAAAT
AATTCTTCAAAATTAAATCATGAATCATTCATATCAAATCTTAATGGAAC
TACAATTAATGAAAATTATTTATTGATTTCACTTCCAATTTTATTAAACA
CATGTATGTCATTTTTATCTGTGAATTTATTCCAACTTGGTTTCGGAAAA
ATTAAAGGAAATTTAGTGGTGAACTATCTTATGGTAATTATACCTTTTAT
TTTGAGTATAACAATATTTTCTAATTACTTGTTGCAAATAACATTGACTG
TTGCTGCTACATCCTTTTTACTTTTAAGCAACAGTTTAATATTGAGCAAA
TCTAATAAATATTTGACTGAAATACGATTAACATTACATTCGGCAAGTAA
CAAACGCGAATCATGGTTGTATATATCCCAAATGAGAGGATTGATCTTAA
TGATGACCACTATTTGCATATTAGCTGTTGATATGAAAGTGTTCCCTAGA
AGGTTTGCAAAAACTGAAATGTTTGGATTTGGACCTATGGACCTTGGAGT
AGGGTTATTCGCAATTATTTATGGGTTACTCGACCAAATTAAAGTTGGTA
GTTTTACAAAACTATCATCTATAGGAATATTATTAGTTTTGGGAATAATG
AGAACTGTATTAGTAAAATATGGAAATTATCAAGAACATGAGAGTGAATA
TGGAACTCATTGGAATTTTTTTTTGACAATGGCAGCTTTAAAATTAGTAT
CGATAATAACAAAATATTTCAGGTTCACCCAACGAATTTTTGGAATCATA
ACTATGATAATGGCAGCCAGTATATTGCAATATTTTCTATTGTATGGATC
TTTTAATCTTGAAAAGTTTTGCAATGATAATAAAGTAGATAGAACAAAGT
CATTGTTAGTGGCAAATAAAGAAGGTTTAATTAGTCTGATTGGTTATATT
GTTTTGTGGTTAACTGGATGTCTACTTGGTGCAATTTTAACAAGAAAATT
TCGTAAATCATTAGCTGTATTTGCAGCTTTAGCAACTATTGGTCTATATA
TTTCTCAGTGCTTTTTACATAAAGACATTGTTTCAAGACGAGCTGCAAAT
TTCGGATATGTTTTATGGACGACTAGTAGTTTCTGCTTTTGCTTAATTTT
ATTTATAATATCAGATATTTTGATAAACTGGCTTGAAGATAGAGCTAATT
TTGTCAATGATACGGAAAATCCGGATATTTATTGTGTTGTTAATAGGCAA
GCGATGGCATTTTTTCTACTATCAAACATCTTAACTGGTTTAACCAACGT
AACAATAAAAACAATGGAATTAAATAAACTGGAATCTATTACTACTTTAG
TTATATATACTATTCTAGTACTTATTACAACGCCACACATTAACGGCATT
TTATAATTTGTTATTTTATTACTTTGCATGAAATATATTTTTTTTAATTC
AACAA
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protein sequence of SMED30024079-orf-1

>SMED30024079-orf-1 ID=SMED30024079-orf-1|Name=SMED30024079-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=456bp
MLLNNSSKLNHESFISNLNGTTINENYLLISLPILLNTCMSFLSVNLFQL
GFGKIKGNLVVNYLMVIIPFILSITIFSNYLLQITLTVAATSFLLLSNSL
ILSKSNKYLTEIRLTLHSASNKRESWLYISQMRGLILMMTTICILAVDMK
VFPRRFAKTEMFGFGPMDLGVGLFAIIYGLLDQIKVGSFTKLSSIGILLV
LGIMRTVLVKYGNYQEHESEYGTHWNFFLTMAALKLVSIITKYFRFTQRI
FGIITMIMAASILQYFLLYGSFNLEKFCNDNKVDRTKSLLVANKEGLISL
IGYIVLWLTGCLLGAILTRKFRKSLAVFAALATIGLYISQCFLHKDIVSR
RAANFGYVLWTTSSFCFCLILFIISDILINWLEDRANFVNDTENPDIYCV
VNRQAMAFFLLSNILTGLTNVTIKTMELNKLESITTLVIYTILVLITTPH
INGIL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR009447PIGW/GWT1
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0005783endoplasmic reticulum
GO:0005789endoplasmic reticulum membrane
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0006506GPI anchor biosynthetic process
Vocabulary: molecular function
TermDefinition
GO:0016746transferase activity, transferring acyl groups
GO:0016740transferase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009447Phosphatidylinositol anchor biosynthesis protein PIGW/GWT1PFAMPF06423GWT1coord: 292..420
e-value: 6.2E-23
score: 81.6
IPR009447Phosphatidylinositol anchor biosynthesis protein PIGW/GWT1PANTHERPTHR20661FAMILY NOT NAMEDcoord: 5..447
NoneNo IPR availableTMHMMTMhelixcoord: 250..272
NoneNo IPR availableTMHMMTMhelixcoord: 81..103
NoneNo IPR availableTMHMMTMhelixcoord: 161..180
NoneNo IPR availableTMHMMTMhelixcoord: 325..342
NoneNo IPR availableTMHMMTMhelixcoord: 187..209
NoneNo IPR availableTMHMMTMhelixcoord: 55..77
NoneNo IPR availableTMHMMTMhelixcoord: 357..379
NoneNo IPR availableTMHMMTMhelixcoord: 124..146
NoneNo IPR availableTMHMMTMhelixcoord: 432..454
NoneNo IPR availableTMHMMTMhelixcoord: 26..48
NoneNo IPR availableTMHMMTMhelixcoord: 400..422
NoneNo IPR availableTMHMMTMhelixcoord: 224..243
NoneNo IPR availableTMHMMTMhelixcoord: 296..318