Protein argonaute-3

Overview
NameProtein argonaute-3
Smed IDSMED30024072
Length (bp)3458
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Protein argonaute-3 (SMED30024072) t-SNE clustered cells

Violin plots show distribution of expression levels for Protein argonaute-3 (SMED30024072) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Protein argonaute-3 (SMED30024072) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Protein argonaute-3 (SMED30024072) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30024072

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30024072SMESG000051000.1 dd_Smed_v4_7594_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30024072SMESG000051000.1 dd_Smed_v4_7594_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30024072SMESG000051000.1 dd_Smed_v4_7594_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30024072SMESG000051000.1 Contig28733newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30024072SMESG000051000.1 Contig28733uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30024072SMESG000051000.1 Contig36070newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30024072SMESG000051000.1 Contig36070uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30024072SMESG000051000.1 OX_Smed_1.0.06956ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
zeta neoblastSMED30024072SMESG000051000.1 dd_Smed_v4_7594_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Protein argonaute-3 vs. Ensembl Human
Match: AGO2 (argonaute RISC catalytic component 2 [Source:HGNC Symbol;Acc:HGNC:3263])

HSP 1 Score: 352.443 bits (903), Expect = 4.993e-105
Identity = 249/845 (29.47%), Postives = 411/845 (48.64%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCG--LPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSG-KGRSNNIPQETIRVLDIIIREMLLDCDLILIG------NKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXX------XXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            P RPD  GT GR I L  N F M +    I+ Y++++   +     N          ++I   +V  F     G   P+FDG + ++    LP      +L      E ++    ++++ V+           +H    GR  ++P ETI+ LD+++R  L       +G      ++G  NP    +       +  G+  ++RP +  +  N+D +    ++A  + +++ +V    +   Q K   +S+       I  +KV+ +H          R + V  V ++ A ++         + +  +V +YF   H + L+YP L  ++     K    P E+  I+  Q+ +     N    +I+ +A    +R + + K ++    F  + Y++ + I +  +M +V GR+L  P+ L+G + + +      + + + K  FH  I+   WA+  F  +R+   E  L +   +L K +    + I     FC  +                      ++V++      Y  VK +GD  LG+ TQC+  +N+  Q+   Q + NL LK+N KLGGVN  +      P+    ++F G DVTHP   DG   S+AAVVGS++     Y A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  +IFYRDGVSEGQF+ V +HE+ AI+ AC K +K+Y+P IT+I+VQKRH+ R       + VG+  N+  G  +D  ITH  EFDFYLCSH GIQGTS+P HY+V+ D+NR++S++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:   26 PPRPD-FGTSGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVN----------REIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQA-----LHDALSGRLPSVPFETIQALDVVMRH-LPSMRYTPVGRSFFTASEGCSNPLGGGR------EVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQ-----MKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISK-LMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQ-FHTGIEIKVWAIACFAPQRQC-TEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNV--QRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQ-----EIIQDLAAMVRELLIQFYKSTRFK-PTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTS 831          
BLAST of Protein argonaute-3 vs. Ensembl Human
Match: AGO3 (argonaute RISC catalytic component 3 [Source:HGNC Symbol;Acc:HGNC:18421])

HSP 1 Score: 328.176 bits (840), Expect = 3.698e-96
Identity = 235/849 (27.68%), Postives = 400/849 (47.11%), Query Frame = 3
Query:  477 FLPERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCG--LPIFDGGRIIFNNQLLPYTETINKLNFIYNYEN-QNINCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQE--------TIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXX------XXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVN-WEIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
             +P RP  G T G+ I L+ NCF + +    +++Y++++   +     N          +++   +V  F     G   P++DG R ++    LP   T   L+     E  ++    ++++ V+      L +       GR+   P E         +  +D+++R  L       +G      P            +  G+  ++RP M  +  N+D +    ++A  +  ++ +V  ++N + Q +   +S        I  +KV+ +H    +     R + V  V ++ A ++         + + ++V +YF   + + LKYP L  ++     K    P E+  I+  Q+ +     N    +IK +A    +R + + + +V    +  + ++Q +   +  +M +V GR+L  P   +G +   +      + + + K  FH  ++   WA+  F  +R+   E  L     +L K +    + I     FC  +                      ++V++      Y  VK +GD  LG+ TQC+  +N+   K   Q + NL LK+N KLGG+N   + +   +  +   +  G DVTHP   DG   S+AAVVGS++   + Y A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  +IFYRDGVSEGQF  V  +E+ AI+ AC   +K+Y+P ITYI+VQKRH+ R       + VGR  N+  G  +D DITH  EFDFYLCSH GIQGTS+P HY+V+ D+N + +++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:   16 MVPRRPGYG-TMGKPIKLLANCFQVEIPKIDVYLYEVDIKPDKCPRRVN----------REVVDSMVQHFKVTIFGDRRPVYDGKRSLYTANPLPVATTGVDLDVTLPGEGGKDRPFKVSIKFVSRVSWHLLHEVLT----GRTLPEPLELDKPISTNPVHAVDVVLRH-LPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMR-----RKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYTLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISR-LVRSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQ-FHTGVEIKMWAIACFATQRQC-REEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVI--KTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQ-----EIIQDLASMVRELLIQFYKSTRFK-PTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVS 832          
BLAST of Protein argonaute-3 vs. Ensembl Human
Match: AGO4 (argonaute RISC component 4 [Source:HGNC Symbol;Acc:HGNC:18424])

HSP 1 Score: 316.62 bits (810), Expect = 4.856e-92
Identity = 241/847 (28.45%), Postives = 399/847 (47.11%), Query Frame = 3
Query:  477 FLPERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGLPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEI----IEN---LGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQ----KVMGNDPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXXXXXXXXXXXXXXX------XXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKD-----AVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIR-FCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            F P R    GT G+ I L+ N F + +    ++ YD+++ K E       R+  + +      R    Q   D+   P +DG R ++    LP       +      E ++       ++    + +  +   + +  G  N +P ++++ LD+I R  L       +G      P            +  G+  ++RP M N+  N+D +    ++A  + +++ +V  + N N Q K   +S+       I  +KV+ +H          R + V  V ++ A ++     +EN   +  +V +YF   + + LKYP L  ++     K    P E+  I+  Q    K+  N  + + +    SA    E    L KS     G   + YL+ + I +  +M  + GR+L  P   +G + + +      + + + K  F+  I+   WA+  F  +++   E+ L +   +L K +    + I     FC  +              +  + + +           Y  VK +GD  LG+ TQC+  +N+   K   Q + NL LK+NAKLGG+N  +   +   +    ++F G DVTHP   DG   S+AAVVGS++   + Y A ++ Q S+         + E++ ++ +MV+ L+  FY   + K P  +I+YR GVSEGQ + V   E+ AI+ AC   +++Y+P ITYI+VQKRH+ R FC    E  VG+  NV  G  +D+ ITH  EFDFYLCSH GIQGTS+P HY V+ D+N + +++LQ  TY LC+   RCTRS+S PAP +YA L AFR R    D+D+ S    + S
Sbjct:   13 FQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDVDI-KPEKRPRRVNREVVDTM-----VRHFKMQIFGDR--QPGYDGKRNMYTAHPLPIGRDRVDMEVTLPGEGKD----QTFKVSVQWVSVVSLQLLLEALAGHLNEVPDDSVQALDVITRH-LPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQ-----MKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGG--PDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQ-FYAGIEIKVWAVACFAPQKQC-REDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVV--KTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEISQELLYSQEVIQDLTNMVRELLIQFYKSTRFK-PTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKTE-RVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVS 833          
BLAST of Protein argonaute-3 vs. Ensembl Human
Match: AGO1 (argonaute RISC component 1 [Source:HGNC Symbol;Acc:HGNC:3262])

HSP 1 Score: 308.145 bits (788), Expect = 4.145e-89
Identity = 229/831 (27.56%), Postives = 393/831 (47.29%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELL--KAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGLPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXX------XXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVN-WEIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNIS 2885
            P RP  G T G+ I L+ N F + +    ++ Y++++   K    +   V ++  +  +  IF D            P++DG + I+    LP              E ++    ++++ +       L +  + SG+     +P E+++ LD+ +R  L       +G      P            +  G+  ++RP M  +  N+D +    ++A  + +++ +V  + N + Q K   +S+       I  +KV+ +H          R + V  V ++ A ++         + +  +V +YF   + + LKYP L  ++     K    P E+  I+  Q+ +     N    +IK +A    +R + + + ++    +  + Y+Q + I +   M  V GR+L  P   +G +   +      + + + K  F++ I+   WA+  F  +++   E  L     +L K +    + I     FC  +                      ++V++      Y  VK +GD  LG+ TQC+  +N+   K   Q + NL LK+N KLGG+N   + +   A  +   +  G DVTHP   DG   S+ AVVGS++   + Y A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  +IFYRDGV EGQ   + ++E+ AI+ AC K +K+Y+P ITYI+VQKRH+ R       + +G+  N+  G  +D +ITH  EFDFYLCSH GIQGTS+P HYYV+ D+NR+ +++LQ  TY LC+   RCTRS+S PAP +YA L AFR R    D+++ S
Sbjct:   24 PRRPGIG-TVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGD----------RKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALV-SGQ---IPVPLESVQALDVAMRH-LASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQ-----MKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISR-LMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQ-FYNGIEIKVWAIACFAPQKQ-CREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVV--KTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQ-----EIIEDLSYMVRELLIQFYKSTRFK-PTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDS 822          
BLAST of Protein argonaute-3 vs. Ensembl Human
Match: AGO3 (argonaute RISC catalytic component 3 [Source:HGNC Symbol;Acc:HGNC:18421])

HSP 1 Score: 298.13 bits (762), Expect = 1.094e-87
Identity = 179/541 (33.09%), Postives = 278/541 (51.39%), Query Frame = 3
Query: 1326 ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXXXXXXXXXXXXXXX------XXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVN-WEIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            + + ++V +YF   + + LKYP L  ++     K    P E+  I+  Q+ +     N    +IK +A    +R + + + +V    +  + ++Q +   +  +M +V GR+L  P   +G +   +      + + + K  FH  ++   WA+  F  +R+   E  L     +L K +    + I     FC  +                   + +           Y  VK +GD  LG+ TQC+  +N+   K   Q + NL LK+N KLGG+N   + +   +  +   +  G DVTHP   DG   S+AAVVGS++   + Y A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  +IFYRDGVSEGQF  V  +E+ AI+ AC   +K+Y+P ITYI+VQKRH+ R       + VGR  N+  G  +D DITH  EFDFYLCSH GIQGTS+P HY+V+ D+N + +++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:   69 QTVERTVAQYFREKYTLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISR-LVRSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQ-FHTGVEIKMWAIACFATQRQC-REEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVI--KTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQ-----EIIQDLASMVRELLIQFYKSTRFK-PTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVS 598          
BLAST of Protein argonaute-3 vs. Ensembl Celegans
Match: alg-2 (pep chromosome:WBcel235:II:874160:879001:1 gene:WBGene00000106.1 transcript:T07D3.7b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:alg-2)

HSP 1 Score: 327.791 bits (839), Expect = 3.478e-96
Identity = 242/854 (28.34%), Postives = 408/854 (47.78%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRK---DIFRDVVSQFLKDKCGL-PIFDGGRIIFNNQLLPY-TETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNP----RTSKQLDRDP-----YTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHIN--------SRIYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHN-------EIIENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGNDPNI-VREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEK---LP-KSKFNIMNKKFKDPFHDPIKWALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFC-YVSNXXXXXXXXXXXXXXXXXXXXXXXXXE-----YKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNW-EIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENESIG 2912
            P RP+ G  +GR I L  N F + +    +  Y I++             F +K  R+   ++   ++S F K    + P++DG R ++  + LP  TE +N    +           + ++ +       L D      +GR   +P E ++ +D+I+R  L       +G +  F P    +   Q+ ++        +  G+  ++RP    +  N+D +    ++A+ + +++ +V  L    +  ++ ++          I  +K++ +H    +     R + V  V ++ A         E  + +  +V +YF   + I LKYP L  ++     K    P E+  I+P Q+ +    ++    +IK +A    ER   + K +V+      + +   + ITI P M  VKGR+L  P  L+G +      LP +  +++  K+F     +   WA+  F ++  +  EN+L     +L + +    + I+    FC Y S  +  + + K  +     + ++   +      Y  VK +GD  LGI TQC+  +N  R     Q + NL LK+N KLGGVN   + N+         +  G D+THP+  D    S+AAVVGS++   + Y+A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  ++ YRDGVSEGQ   V  +E+ AI+ AC   +  Y+P IT+I VQKRH+ R       D VG+  N+ PG  +D  ITH  EFDF+LCSH GIQGTS+P HY+V+ D+N   +++LQ+ TY +C+   RCTRS+S PAP +YAHL AFR R    D D+ S    ++  G
Sbjct:   46 PVRPNHG-VEGRSILLRANHFAVRIPGGSVQHYQIDV-------------FPDKCPRRVNREVIGCLISSFSKYFTNIRPVYDGKRNMYTREPLPIGTEPMNFEVTLPGDSAVERKFSVTMKWIGQVCLSALDDAM----EGRVRQVPHEAVQSIDVILRH-LPSLKYTPVG-RSFFTPPGVMKPGMQMHQESKLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYRAMPVIEFVAEVLELPVQALAERRALSDAQRVKFTKEIRGLKIEITHCGAVR-----RKYRVCNVTRRPAQTQTFPLQLETGQTIECTVAKYFFDKYRIQLKYPHLPCLQVGQEQKHTYLPPEVCDIVPGQRCLKKLTDVQTSTMIKATARSAPEREREICK-LVSKAELSADPFAHEFGITINPAMTEVKGRVLSAPKLLYGGRHRATTALPNQGVWDMRGKQFHTGM-EVRTWAIACFAQQ-SHVKENDLRMFTTQLQRISTDAGMPIIGTPMFCKYASGVEQVEPMFKYLKQTYSAIQLIVVVLPGKTPIYAEVKRVGDTVLGIATQCVQAKNAIR--TTPQTLSNLCLKMNVKLGGVNSILLPNVRPRIFNEPVIFLGCDITHPAAGDTRKPSIAAVVGSMDAHPSRYAATVRVQQHRQ-----EIITDLTYMVRELLVQFYRNTRFK-PARIVVYRDGVSEGQLFNVLQYELRAIREACVMLESGYQPGITFIAVQKRHHTRLFAADKADQVGKAFNIPPGTTVDVGITHPTEFDFFLCSHAGIQGTSRPSHYHVLWDDNDLTADELQQLTYQMCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDRDHGSGEEGSQPSG 863          
BLAST of Protein argonaute-3 vs. Ensembl Celegans
Match: alg-2 (pep chromosome:WBcel235:II:873176:878945:1 gene:WBGene00000106.1 transcript:T07D3.7a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:alg-2)

HSP 1 Score: 327.02 bits (837), Expect = 7.804e-96
Identity = 244/856 (28.50%), Postives = 406/856 (47.43%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRK---DIFRDVVSQFLKDKCGL-PIFDGGRIIFNNQLLPY-TETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNP----RTSKQLDRDP-----YTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHIN--------SRIYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHN-------EIIENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGNDPNI-VREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEK---LPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFC-YVSNXXXXXXXXXXXXXXXXXXXXXXXXXE-----YKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNW-EIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENESIG 2912
            P RP+ G  +GR I L  N F + +    +  Y I++             F +K  R+   ++   ++S F K    + P++DG R ++  + LP  TE +N    +           + ++ +       L D      +GR   +P E ++ +D+I+R  L       +G +  F P    +   Q+ ++        +  G+  ++RP    +  N+D +    ++A+ + +++ +V  L    +  ++ ++          I  +K++ +H    +     R + V  V ++ A         E  + +  +V +YF   + I LKYP L  ++     K    P E+  I+P Q+ +    ++    +IK +A    ER   + K +V+      + +   + ITI P M  VKGR+L  P  L+G +      LP      M  K    FH  ++   WA+  F ++  +  EN+L     +L + +    + I+    FC Y S  +  + + K  +     + ++   +      Y  VK +GD  LGI TQC+  +N  R     Q + NL LK+N KLGGVN   + N+         +  G D+THP+  D    S+AAVVGS++   + Y+A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  ++ YRDGVSEGQ   V  +E+ AI+ AC   +  Y+P IT+I VQKRH+ R       D VG+  N+ PG  +D  ITH  EFDF+LCSH GIQGTS+P HY+V+ D+N   +++LQ+ TY +C+   RCTRS+S PAP +YAHL AFR R    D D+ S    ++  G
Sbjct:   65 PVRPNHG-VEGRSILLRANHFAVRIPGGSVQHYQIDV-------------FPDKCPRRVNREVIGCLISSFSKYFTNIRPVYDGKRNMYTREPLPIGTEPMNFEVTLPGDSAVERKFSVTMKWIGQVCLSALDDAM----EGRVRQVPHEAVQSIDVILRH-LPSLKYTPVG-RSFFTPPGVMKPGMQMHQESKLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYRAMPVIEFVAEVLELPVQALAERRALSDAQRVKFTKEIRGLKIEITHCGAVR-----RKYRVCNVTRRPAQTQTFPLQLETGQTIECTVAKYFFDKYRIQLKYPHLPCLQVGQEQKHTYLPPEVCDIVPGQRCLKKLTDVQTSTMIKATARSAPEREREICK-LVSKAELSADPFAHEFGITINPAMTEVKGRVLSAPKLLYGGRHRATTALPNQGVWDMRGK---QFHTGMEVRTWAIACFAQQ-SHVKENDLRMFTTQLQRISTDAGMPIIGTPMFCKYASGVEQVEPMFKYLKQTYSAIQLIVVVLPGKTPIYAEVKRVGDTVLGIATQCVQAKNAIR--TTPQTLSNLCLKMNVKLGGVNSILLPNVRPRIFNEPVIFLGCDITHPAAGDTRKPSIAAVVGSMDAHPSRYAATVRVQQHRQ-----EIITDLTYMVRELLVQFYRNTRFK-PARIVVYRDGVSEGQLFNVLQYELRAIREACVMLESGYQPGITFIAVQKRHHTRLFAADKADQVGKAFNIPPGTTVDVGITHPTEFDFFLCSHAGIQGTSRPSHYHVLWDDNDLTADELQQLTYQMCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDRDHGSGEEGSQPSG 882          
BLAST of Protein argonaute-3 vs. Ensembl Celegans
Match: alg-1 (pep chromosome:WBcel235:X:13951262:13956261:1 gene:WBGene00000105.1 transcript:F48F7.1a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:alg-1)

HSP 1 Score: 322.398 bits (825), Expect = 1.842e-93
Identity = 244/879 (27.76%), Postives = 407/879 (46.30%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGL-PIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNI---NCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNK--------------GLFNPRTSKQLDRDPYT---------------ILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINS--------RIYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHN-------EIIENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGNDPNI-VREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVS------NXXXXXXXXXXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNW-EIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENESIGLPDISIN 2933
            P RP+ G  +GR I L  N F + +    I  Y +++            D   +   ++I   ++S F K    + P++DG R ++  + LP      +++F       +       ++L+ V       L D      +GR   +P E ++ +D+I+R  L       +G                G   P+ S  +    ++               +  G+  ++RP    +  N+D +    ++++ + ++I +V  L    +  ++ ++          I  +K++ +H          R + V  V ++ A         E  + +  +V +YF   + I LKYP L  ++     K    P E+  I+P Q+ +    ++    +IK +A    ER   +  ++V    F  + +   + ITI P M  VKGR+L  P  L+G +          + + + K  FH  I    WA+  F ++ ++  EN+L     +L + +    + I+ +  FC  +          K   +       ++VV+      Y  VK +GD  LGI TQC+  +N  R     Q + NL LK+N KLGGVN   + N+         + FG D+THP   D    S+AAVVGS++   + Y+A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  ++ YRDGVSEGQF  V  +E+ AI+ AC   ++ Y+P IT+I VQKRH+ R   +  +D VG+  N+ PG  +D  ITH  EFDFYLCSH GIQGTS+P HY+V+ D+N   +++LQ+ TY +C+   RCTRS+S PAP +YAHL AFR R    D ++ S      S    D +++
Sbjct:  139 PRRPNHG-VEGRSILLRANHFAVRIPGGTIQHYQVDVTP----------DKCPRRVNREIISCLISAFSKYFTNIRPVYDGKRNMYTREPLPIGR--ERMDFDVTLPGDSAVERQFSVSLKWVGQVSLSTLEDAM----EGRVRQVPFEAVQAMDVILRH-LPSLKYTPVGRSFFSPPVPNASGVMAGSCPPQASGAVAGGAHSAGQYHAESKLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYRSMPVIEFIAEVLELPVQALAERRALSDAQRVKFTKEIRGLKIEITHCGQ-----MRRKYRVCNVTRRPAQTQTFPLQLETGQTIECTVAKYFYDKYRIQLKYPHLPCLQVGQEQKHTYLPPEVCNIVPGQRCIKKLTDVQTSTMIKATARSAPEREREIS-NLVRKAEFSADPFAHEFGITINPAMTEVKGRVLSAPKLLYGGRTRATALPNQGVWDMRGKQ-FHTGIDVRVWAIACFAQQ-QHVKENDLRMFTNQLQRISNDAGMPIVGNPCFCKYAVGVEQVEPMFKYLKQNYSGIQLVVVVLPGKTPVYAEVKRVGDTVLGIATQCVQAKNAIR--TTPQTLSNLCLKMNVKLGGVNSILLPNVRPRIFNEPVIFFGCDITHPPAGDSRKPSIAAVVGSMDAHPSRYAATVRVQQHRQ-----EIISDLTYMVRELLVQFYRNTRFK-PARIVVYRDGVSEGQFFNVLQYELRAIREACMMLERGYQPGITFIAVQKRHHTRLFAVDKKDQVGKAYNIPPGTTVDVGITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNNLTADELQQLTYQMCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDREHDSGEGSQPSGTSEDTTLS 983          
BLAST of Protein argonaute-3 vs. Ensembl Celegans
Match: alg-1 (pep chromosome:WBcel235:X:13949114:13956261:1 gene:WBGene00000105.1 transcript:F48F7.1b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:alg-1)

HSP 1 Score: 322.398 bits (825), Expect = 3.057e-93
Identity = 244/879 (27.76%), Postives = 407/879 (46.30%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGL-PIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNI---NCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNK--------------GLFNPRTSKQLDRDPYT---------------ILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINS--------RIYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHN-------EIIENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGNDPNI-VREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVS------NXXXXXXXXXXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNW-EIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENESIGLPDISIN 2933
            P RP+ G  +GR I L  N F + +    I  Y +++            D   +   ++I   ++S F K    + P++DG R ++  + LP      +++F       +       ++L+ V       L D      +GR   +P E ++ +D+I+R  L       +G                G   P+ S  +    ++               +  G+  ++RP    +  N+D +    ++++ + ++I +V  L    +  ++ ++          I  +K++ +H          R + V  V ++ A         E  + +  +V +YF   + I LKYP L  ++     K    P E+  I+P Q+ +    ++    +IK +A    ER   +  ++V    F  + +   + ITI P M  VKGR+L  P  L+G +          + + + K  FH  I    WA+  F ++ ++  EN+L     +L + +    + I+ +  FC  +          K   +       ++VV+      Y  VK +GD  LGI TQC+  +N  R     Q + NL LK+N KLGGVN   + N+         + FG D+THP   D    S+AAVVGS++   + Y+A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  ++ YRDGVSEGQF  V  +E+ AI+ AC   ++ Y+P IT+I VQKRH+ R   +  +D VG+  N+ PG  +D  ITH  EFDFYLCSH GIQGTS+P HY+V+ D+N   +++LQ+ TY +C+   RCTRS+S PAP +YAHL AFR R    D ++ S      S    D +++
Sbjct:  160 PRRPNHG-VEGRSILLRANHFAVRIPGGTIQHYQVDVTP----------DKCPRRVNREIISCLISAFSKYFTNIRPVYDGKRNMYTREPLPIGR--ERMDFDVTLPGDSAVERQFSVSLKWVGQVSLSTLEDAM----EGRVRQVPFEAVQAMDVILRH-LPSLKYTPVGRSFFSPPVPNASGVMAGSCPPQASGAVAGGAHSAGQYHAESKLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYRSMPVIEFIAEVLELPVQALAERRALSDAQRVKFTKEIRGLKIEITHCGQ-----MRRKYRVCNVTRRPAQTQTFPLQLETGQTIECTVAKYFYDKYRIQLKYPHLPCLQVGQEQKHTYLPPEVCNIVPGQRCIKKLTDVQTSTMIKATARSAPEREREIS-NLVRKAEFSADPFAHEFGITINPAMTEVKGRVLSAPKLLYGGRTRATALPNQGVWDMRGKQ-FHTGIDVRVWAIACFAQQ-QHVKENDLRMFTNQLQRISNDAGMPIVGNPCFCKYAVGVEQVEPMFKYLKQNYSGIQLVVVVLPGKTPVYAEVKRVGDTVLGIATQCVQAKNAIR--TTPQTLSNLCLKMNVKLGGVNSILLPNVRPRIFNEPVIFFGCDITHPPAGDSRKPSIAAVVGSMDAHPSRYAATVRVQQHRQ-----EIISDLTYMVRELLVQFYRNTRFK-PARIVVYRDGVSEGQFFNVLQYELRAIREACMMLERGYQPGITFIAVQKRHHTRLFAVDKKDQVGKAYNIPPGTTVDVGITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNNLTADELQQLTYQMCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDREHDSGEGSQPSGTSEDTTLS 1004          
BLAST of Protein argonaute-3 vs. Ensembl Celegans
Match: alg-4 (pep chromosome:WBcel235:III:9887211:9891355:-1 gene:WBGene00006449.1 transcript:ZK757.3b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:alg-4)

HSP 1 Score: 234.187 bits (596), Expect = 3.974e-63
Identity = 163/520 (31.35%), Postives = 256/520 (49.23%), Query Frame = 3
Query: 1380 LKYPELLLIETTNYNKFPAELLTILPNQKVMGNDPN---------IVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKL-----PKSKFNIMNKKFKDPFHDPIKWALFAFGERREYGN--------ENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXXXXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEI---KNIEYAPIKGLFMVFGVDVTHPSNNDGLSK--SVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIR-FCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGR 2855
            LKYP+L  +        P      LP +  + + P              +IK +A+  T+R D +K+ + A   F  + +L+ + + +  QMI    R++  P  +FG     +     PK     M+ +          +++ A  + R+  +          +  A+ +  P+    V     K++     +    +  +     D  ++V+  +N   Y  VK+  DI  GI++QC+  +N+ R    +    N++LK+N K+GG+N  I   K      +    MV G+DVTHP+  +      SVAA+V +++     Y A +K Q     K   E VV + D ++  I  FY   + K P  +I YRDGVSEGQF  V   EI +I+ AC    ++++P ITYI+VQKRH+ R FC     D VG+ KNV PG  +D  I     FDFYLCSH G+QGTS+P  Y+V+LDE ++ ++++Q  TY +C+   RCTRS+S P P +YA L A R R
Sbjct:  455 LKYPKLPCLHVG-----PPTRNIFLPMEHCLIDSPQKYNKKMSEKQTSAIIKAAAVDATQREDRIKQ-LAAQASFGTDPFLKEFGVAVSSQMIQTTARVIQPPPIMFGGNNRSVNPVVFPKDGSWTMDNQTLYMPATCRSYSMIALVDPRDQTSLQTFCQSLTMKATAMGMNFPRWPDLVKYGRSKEDVCTLFTEIADEYRVTNTVCDCIIVVLQSKNSDIYMTVKEQSDIVHGIMSQCVLMKNVSRPTPAT--CANIVLKLNMKMGGINSRIVADKITNKYLVDQPTMVVGIDVTHPTQAEMRMNMPSVAAIVANVDLLPQSYGANVKVQ-----KKCRESVVYLLDAIRERIITFYRHTKQK-PARIIVYRDGVSEGQFSEVLREEIQSIRTACLAIAEDFRPPITYIVVQKRHHARIFCKYQ-NDMVGKAKNVPPGTTVDTGIVSPEGFDFYLCSHYGVQGTSRPARYHVLLDECKFTADEIQSITYGMCHTYGRCTRSVSIPTPVYYADLVATRAR 959          
BLAST of Protein argonaute-3 vs. Ensembl Fly
Match: AGO1 (gene:FBgn0262739 transcript:FBtr0305592)

HSP 1 Score: 322.783 bits (826), Expect = 1.435e-93
Identity = 246/842 (29.22%), Postives = 400/842 (47.51%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGL-PIFDGGRIIFNNQLLPYTETINKLNFIYNYENQN------INCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGNDPNI-VREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDK-------EEKLP-----------KSKFNIMNKKFKDPFHDPIKWALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFC-YVSNXXXXXXXXXXXXXXXXXXXXXXXXXE-----YKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNW-EIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIR-FCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGR 2855
            P RP+ G  +GR I L  N F +T+    +  YDI +   +   + N          ++I   +V  + K    L P+FDG   ++    LP      +L      E ++      I     + L N +  L          +GR+  IP + I  LD+++R  L       +G     +P            +  G+  ++RP    +  N+D +    ++A  + D++ +V  + + N Q K   +S+       I  +K++ +H    +     R + V  V ++ A  +         + +  +V +YFL  + + L+YP L  ++    +K    P E+  I+  Q+ +    ++    +IK +A    +R   +  ++V    F N+ Y+Q + +TI   M+ V+GR+L  P   +G +       ++  P           +  +++  K+F       I WA+  F  +R    E+ L     +L K +    + I+    FC Y +  D  + + +  +     + +V   +      Y  VK +GD  LG+ TQC+  +N+   K   Q + NL LK+N KLGG+N   + +I         +  G DVTHP   D    S+AAVVGS++   + Y+A ++ Q  +      EI+ E+  MV+ L+ +FY       P  +I YRDGVSEGQF  V  HE+ AI+ AC K +  Y+P IT+I+VQKRH+ R FC    E S G+  N+  G  +D  ITH  EFDFYLCSH+GIQGTS+P HY+V+ D+N ++S++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R
Sbjct:  136 PRRPNLG-REGRPIVLRANHFQVTMPRGYVHHYDINIQPDKCPRKVN----------REIIETMVHAYSKIFGVLKPVFDGRNNLYTRDPLPIGNERLELEVTLPGEGKDRIFRVTIKWQAQVSLFNLEEAL----------EGRTRQIPYDAILALDVVMRH-LPSMTYTPVGRSFFSSPEGYYHPLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYKAQPVIDFMCEVLDIRDINEQRKPLTDSQRVKFTKEIKGLKIEITHCGQMR-----RKYRVCNVTRRPAQMQSFPLQLENGQTVECTVAKYFLDKYRMKLRYPHLPCLQVGQEHKHTYLPLEVCNIVAGQRCIKKLTDMQTSTMIKATARSAPDREREIN-NLVKRADFNNDSYVQEFGLTISNSMMEVRGRVLPPPKLQYGGRVSTGLTGQQLFPPQNKVSLASPNQGVWDMRGKQFFTGVEIRI-WAIACFAPQRTV-REDALRNFTQQLQKISNDAGMPIIGQPCFCKYATGPDQVEPMFRYLKITFPGLQLVVVVLPGKTPVYAEVKRVGDTVLGMATQCVQAKNV--NKTSPQTLSNLCLKINVKLGGINSILVPSIRPKVFNEPVIFLGADVTHPPAGDNKKPSIAAVVGSMDAHPSRYAATVRVQQHRQ-----EIIQELSSMVRELLIMFYKSTGGYKPHRIILYRDGVSEGQFPHVLQHELTAIREACIKLEPEYRPGITFIVVQKRHHTRLFCAEKKEQS-GKSGNIPAGTTVDVGITHPTEFDFYLCSHQGIQGTSRPSHYHVLWDDNHFDSDELQCLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRAR 939          
BLAST of Protein argonaute-3 vs. Ensembl Fly
Match: AGO1 (gene:FBgn0262739 transcript:FBtr0087612)

HSP 1 Score: 322.783 bits (826), Expect = 1.435e-93
Identity = 246/842 (29.22%), Postives = 400/842 (47.51%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGL-PIFDGGRIIFNNQLLPYTETINKLNFIYNYENQN------INCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGNDPNI-VREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDK-------EEKLP-----------KSKFNIMNKKFKDPFHDPIKWALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFC-YVSNXXXXXXXXXXXXXXXXXXXXXXXXXE-----YKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNW-EIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIR-FCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGR 2855
            P RP+ G  +GR I L  N F +T+    +  YDI +   +   + N          ++I   +V  + K    L P+FDG   ++    LP      +L      E ++      I     + L N +  L          +GR+  IP + I  LD+++R  L       +G     +P            +  G+  ++RP    +  N+D +    ++A  + D++ +V  + + N Q K   +S+       I  +K++ +H    +     R + V  V ++ A  +         + +  +V +YFL  + + L+YP L  ++    +K    P E+  I+  Q+ +    ++    +IK +A    +R   +  ++V    F N+ Y+Q + +TI   M+ V+GR+L  P   +G +       ++  P           +  +++  K+F       I WA+  F  +R    E+ L     +L K +    + I+    FC Y +  D  + + +  +     + +V   +      Y  VK +GD  LG+ TQC+  +N+   K   Q + NL LK+N KLGG+N   + +I         +  G DVTHP   D    S+AAVVGS++   + Y+A ++ Q  +      EI+ E+  MV+ L+ +FY       P  +I YRDGVSEGQF  V  HE+ AI+ AC K +  Y+P IT+I+VQKRH+ R FC    E S G+  N+  G  +D  ITH  EFDFYLCSH+GIQGTS+P HY+V+ D+N ++S++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R
Sbjct:  136 PRRPNLG-REGRPIVLRANHFQVTMPRGYVHHYDINIQPDKCPRKVN----------REIIETMVHAYSKIFGVLKPVFDGRNNLYTRDPLPIGNERLELEVTLPGEGKDRIFRVTIKWQAQVSLFNLEEAL----------EGRTRQIPYDAILALDVVMRH-LPSMTYTPVGRSFFSSPEGYYHPLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYKAQPVIDFMCEVLDIRDINEQRKPLTDSQRVKFTKEIKGLKIEITHCGQMR-----RKYRVCNVTRRPAQMQSFPLQLENGQTVECTVAKYFLDKYRMKLRYPHLPCLQVGQEHKHTYLPLEVCNIVAGQRCIKKLTDMQTSTMIKATARSAPDREREIN-NLVKRADFNNDSYVQEFGLTISNSMMEVRGRVLPPPKLQYGGRVSTGLTGQQLFPPQNKVSLASPNQGVWDMRGKQFFTGVEIRI-WAIACFAPQRTV-REDALRNFTQQLQKISNDAGMPIIGQPCFCKYATGPDQVEPMFRYLKITFPGLQLVVVVLPGKTPVYAEVKRVGDTVLGMATQCVQAKNV--NKTSPQTLSNLCLKINVKLGGINSILVPSIRPKVFNEPVIFLGADVTHPPAGDNKKPSIAAVVGSMDAHPSRYAATVRVQQHRQ-----EIIQELSSMVRELLIMFYKSTGGYKPHRIILYRDGVSEGQFPHVLQHELTAIREACIKLEPEYRPGITFIVVQKRHHTRLFCAEKKEQS-GKSGNIPAGTTVDVGITHPTEFDFYLCSHQGIQGTSRPSHYHVLWDDNHFDSDELQCLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRAR 939          
BLAST of Protein argonaute-3 vs. Ensembl Fly
Match: AGO1 (gene:FBgn0262739 transcript:FBtr0087613)

HSP 1 Score: 322.783 bits (826), Expect = 1.435e-93
Identity = 246/842 (29.22%), Postives = 400/842 (47.51%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGL-PIFDGGRIIFNNQLLPYTETINKLNFIYNYENQN------INCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGNDPNI-VREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDK-------EEKLP-----------KSKFNIMNKKFKDPFHDPIKWALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFC-YVSNXXXXXXXXXXXXXXXXXXXXXXXXXE-----YKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNW-EIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIR-FCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGR 2855
            P RP+ G  +GR I L  N F +T+    +  YDI +   +   + N          ++I   +V  + K    L P+FDG   ++    LP      +L      E ++      I     + L N +  L          +GR+  IP + I  LD+++R  L       +G     +P            +  G+  ++RP    +  N+D +    ++A  + D++ +V  + + N Q K   +S+       I  +K++ +H    +     R + V  V ++ A  +         + +  +V +YFL  + + L+YP L  ++    +K    P E+  I+  Q+ +    ++    +IK +A    +R   +  ++V    F N+ Y+Q + +TI   M+ V+GR+L  P   +G +       ++  P           +  +++  K+F       I WA+  F  +R    E+ L     +L K +    + I+    FC Y +  D  + + +  +     + +V   +      Y  VK +GD  LG+ TQC+  +N+   K   Q + NL LK+N KLGG+N   + +I         +  G DVTHP   D    S+AAVVGS++   + Y+A ++ Q  +      EI+ E+  MV+ L+ +FY       P  +I YRDGVSEGQF  V  HE+ AI+ AC K +  Y+P IT+I+VQKRH+ R FC    E S G+  N+  G  +D  ITH  EFDFYLCSH+GIQGTS+P HY+V+ D+N ++S++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R
Sbjct:  136 PRRPNLG-REGRPIVLRANHFQVTMPRGYVHHYDINIQPDKCPRKVN----------REIIETMVHAYSKIFGVLKPVFDGRNNLYTRDPLPIGNERLELEVTLPGEGKDRIFRVTIKWQAQVSLFNLEEAL----------EGRTRQIPYDAILALDVVMRH-LPSMTYTPVGRSFFSSPEGYYHPLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYKAQPVIDFMCEVLDIRDINEQRKPLTDSQRVKFTKEIKGLKIEITHCGQMR-----RKYRVCNVTRRPAQMQSFPLQLENGQTVECTVAKYFLDKYRMKLRYPHLPCLQVGQEHKHTYLPLEVCNIVAGQRCIKKLTDMQTSTMIKATARSAPDREREIN-NLVKRADFNNDSYVQEFGLTISNSMMEVRGRVLPPPKLQYGGRVSTGLTGQQLFPPQNKVSLASPNQGVWDMRGKQFFTGVEIRI-WAIACFAPQRTV-REDALRNFTQQLQKISNDAGMPIIGQPCFCKYATGPDQVEPMFRYLKITFPGLQLVVVVLPGKTPVYAEVKRVGDTVLGMATQCVQAKNV--NKTSPQTLSNLCLKINVKLGGINSILVPSIRPKVFNEPVIFLGADVTHPPAGDNKKPSIAAVVGSMDAHPSRYAATVRVQQHRQ-----EIIQELSSMVRELLIMFYKSTGGYKPHRIILYRDGVSEGQFPHVLQHELTAIREACIKLEPEYRPGITFIVVQKRHHTRLFCAEKKEQS-GKSGNIPAGTTVDVGITHPTEFDFYLCSHQGIQGTSRPSHYHVLWDDNHFDSDELQCLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRAR 939          
BLAST of Protein argonaute-3 vs. Ensembl Fly
Match: AGO1 (gene:FBgn0262739 transcript:FBtr0087614)

HSP 1 Score: 321.627 bits (823), Expect = 1.846e-93
Identity = 246/842 (29.22%), Postives = 400/842 (47.51%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGL-PIFDGGRIIFNNQLLPYTETINKLNFIYNYENQN------INCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGNDPNI-VREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDK-------EEKLP-----------KSKFNIMNKKFKDPFHDPIKWALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFC-YVSNXXXXXXXXXXXXXXXXXXXXXXXXXE-----YKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNW-EIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIR-FCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGR 2855
            P RP+ G  +GR I L  N F +T+    +  YDI +   +   + N          ++I   +V  + K    L P+FDG   ++    LP      +L      E ++      I     + L N +  L          +GR+  IP + I  LD+++R  L       +G     +P            +  G+  ++RP    +  N+D +    ++A  + D++ +V  + + N Q K   +S+       I  +K++ +H    +     R + V  V ++ A  +         + +  +V +YFL  + + L+YP L  ++    +K    P E+  I+  Q+ +    ++    +IK +A    +R   +  ++V    F N+ Y+Q + +TI   M+ V+GR+L  P   +G +       ++  P           +  +++  K+F       I WA+  F  +R    E+ L     +L K +    + I+    FC Y +  D  + + +  +     + +V   +      Y  VK +GD  LG+ TQC+  +N+   K   Q + NL LK+N KLGG+N   + +I         +  G DVTHP   D    S+AAVVGS++   + Y+A ++ Q  +      EI+ E+  MV+ L+ +FY       P  +I YRDGVSEGQF  V  HE+ AI+ AC K +  Y+P IT+I+VQKRH+ R FC    E S G+  N+  G  +D  ITH  EFDFYLCSH+GIQGTS+P HY+V+ D+N ++S++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R
Sbjct:  102 PRRPNLG-REGRPIVLRANHFQVTMPRGYVHHYDINIQPDKCPRKVN----------REIIETMVHAYSKIFGVLKPVFDGRNNLYTRDPLPIGNERLELEVTLPGEGKDRIFRVTIKWQAQVSLFNLEEAL----------EGRTRQIPYDAILALDVVMRH-LPSMTYTPVGRSFFSSPEGYYHPLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYKAQPVIDFMCEVLDIRDINEQRKPLTDSQRVKFTKEIKGLKIEITHCGQMR-----RKYRVCNVTRRPAQMQSFPLQLENGQTVECTVAKYFLDKYRMKLRYPHLPCLQVGQEHKHTYLPLEVCNIVAGQRCIKKLTDMQTSTMIKATARSAPDREREIN-NLVKRADFNNDSYVQEFGLTISNSMMEVRGRVLPPPKLQYGGRVSTGLTGQQLFPPQNKVSLASPNQGVWDMRGKQFFTGVEIRI-WAIACFAPQRTV-REDALRNFTQQLQKISNDAGMPIIGQPCFCKYATGPDQVEPMFRYLKITFPGLQLVVVVLPGKTPVYAEVKRVGDTVLGMATQCVQAKNV--NKTSPQTLSNLCLKINVKLGGINSILVPSIRPKVFNEPVIFLGADVTHPPAGDNKKPSIAAVVGSMDAHPSRYAATVRVQQHRQ-----EIIQELSSMVRELLIMFYKSTGGYKPHRIILYRDGVSEGQFPHVLQHELTAIREACIKLEPEYRPGITFIVVQKRHHTRLFCAEKKEQS-GKSGNIPAGTTVDVGITHPTEFDFYLCSHQGIQGTSRPSHYHVLWDDNHFDSDELQCLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRAR 905          
BLAST of Protein argonaute-3 vs. Ensembl Fly
Match: AGO2 (gene:FBgn0087035 transcript:FBtr0331933)

HSP 1 Score: 208.764 bits (530), Expect = 1.165e-55
Identity = 157/508 (30.91%), Postives = 253/508 (49.80%), Query Frame = 3
Query: 1380 LKYPELLLIETTNYNK---FPAELLTILPNQKVMGND-PNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNN--KYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKS-KFNIMNKKFKDPFHDPIKWALF----AFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXXXXXXXXXXXXXXXXXXXEYKL----VKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRI 2858
            LK+P+L  +   +  K    P EL +I   Q +   D    V  +IK++A     R    +K +   + F++N    +  + I I    I V  R+L  P   +  K   + K+  + +   KF +P     K A+       G +  Y   N+   + I   KA   VN+ +  D  +   ++D+                + +    ++++    +K   +++ GI+TQCI    + R+ N +Q +GN++LK+N+KL G+N +IK+    P+    M  G DVTHPS +     SV  V  S +     Y+   + Q     + A+E + +M  +    ++V+ +  ++  PDH+I+YRDGVS+GQF  + N E+  I+ AC+K     KPKI  +IV KRH+ RF P     +  +  NV PG V+D  I H  E  F++ SH+ IQGT+KP  Y VI +    + + LQ+ TY LC++  RC RS+SYPAP + AHL A RGR+
Sbjct:  262 LKFPQLHCLNVGSSIKSILLPIELCSIEEGQALNRKDGATQVANMIKYAATSTNVR---KRKIMNLLQYFQHNLDPTISRFGIRIANDFIVVSTRVLSPPQVEYHSKRFTMVKNGSWRMDGMKFLEPKPKAHKCAVLYCDPRSGRKMNYTQLNDFGNLIISQGKA---VNISLDSDVTYRPFTDDERSLDTIFADLKRSQHDLAIVIIPQFRISYDTIKQKAELQHGILTQCIKQFTVERKCN-NQTIGNILLKINSKLNGINHKIKDDPRLPMMKNTMYIGADVTHPSPDQREIPSVVGVAASHDPYGASYNMQYRLQ-----RGALEEIEDMFSITLEHLRVYKEY-RNAYPDHIIYYRDGVSDGQFPKIKNEELRCIKQACDKV--GCKPKICCVIVVKRHHTRFFPSGDVTTSNKFNNVDPGTVVDRTIVHPNEMQFFMVSHQAIQGTAKPTRYNVIENTGNLDIDLLQQLTYNLCHMFPRCNRSVSYPAPAYLAHLVAARGRV 754          
BLAST of Protein argonaute-3 vs. Ensembl Zebrafish
Match: ago2 (argonaute RISC catalytic component 2 [Source:ZFIN;Acc:ZDB-GENE-110606-6])

HSP 1 Score: 342.813 bits (878), Expect = 1.320e-101
Identity = 248/851 (29.14%), Postives = 411/851 (48.30%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCG--LPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSG-KGRSNNIPQETIRVLDIIIREMLLDCDLILIG------NKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXX------XXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-------GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            P+RPD  GT GR I L  N F M +   +++ Y+I++   +     N          ++I   +V  F     G   P++DG + ++    LP      +L      E ++ +  + ++ ++           +H    GR  NIP ETI+ LD+++R  L       +G      ++G  NP    +       +  G+  ++RP +  +  N+D +    ++A  + +++ +V    +   Q K   +S+       I  +KV+ +H          R + V  V ++ A ++         + +  +V +YF   + + L+YP L  ++     K    P E+  I+  Q+ +     N    +I+ +A    +R D + K ++    F  + Y++ + + +   M  V GR+L  P+ L+G + + +      + + + K  FH  I+   WA+  F  +R+   E  L A   +L K +    + I     FC  +                 +    ++V++      Y  VK +GD  LG+ TQC+  +N+  QK   Q + NL LK+N KLGGVN    NI     +G  +VF       G DVTHP   DG   S+AAVVGS++   + Y A ++ Q  +      +I+ ++  MV+ L+  FY   + K P  +I+YRDG+SEGQF  V  HE+ AI+ AC K +K+Y+P IT+++VQKRH+ R   +   + VG+  N+  G  +D  ITH  EFDFYLCSH GIQGTS+P HY+V+ D+N + S++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:   40 PQRPD-FGTMGRTIKLQANFFEMEIPKLEVYHYEIDIKPEKCPRRVN----------REIVEHMVQHFKTQIFGDRKPVYDGRKNLYTAMPLPIGRDKVELEVTIPGEGKDRSFKVAIKWMSCVSLQA-----LHEALSGRLPNIPFETIQALDVVMRH-LPSMRYTPVGRSFFTPSEGCSNPLGGGR------EVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFMCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQ-----MKRKYRVCNVTRRPASHQTFPLQQENGQTIECTVAQYFKDKYKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQDEISK-LMRSANFNTDPYVREFGVMVRDDMTEVNGRVLQAPSILYGGRNKAIATPVQGVWDMRNKQ-FHTGIEIKVWAIACFAPQRQC-TELLLKAFTDQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFKHLKYTYQGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQVKNV--QKTTPQTLSNLCLKINVKLGGVN----NILLP--QGRPLVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQQHRQ-----DIIQDLATMVRELLIQFYKSTRFK-PTRIIYYRDGISEGQFNQVLQHELLAIREACIKLEKDYQPGITFVVVQKRHHTRLFCMDRNERVGKSGNIPAGTTVDTKITHPFEFDFYLCSHAGIQGTSRPSHYHVLWDDNHFTSDELQVLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTS 845          
BLAST of Protein argonaute-3 vs. Ensembl Zebrafish
Match: ago3b (argonaute RISC catalytic component 3b [Source:NCBI gene;Acc:568159])

HSP 1 Score: 330.487 bits (846), Expect = 2.782e-97
Identity = 235/848 (27.71%), Postives = 392/848 (46.23%), Query Frame = 3
Query:  480 LPERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCG--LPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINC-HLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQE--------TIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXXXXXXXXXXXXXX------XXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            +P RP  G T G+ I L+ NCF + +    +++YD+++   +     N          +++   +V  F     G   P++DG + ++  Q LP       L+     E        + ++ V+      L +       GRS   P E         +  +D+++R  L       +G     +P            +  G+  ++RP M  +  N+D +    ++A  +  ++ +V  ++N + Q +   +S        I  +KV+ +H    +     R + V  V ++ A ++         + + ++V +YF   + + LKYP L  ++     K    P E+  I+  Q+ +     N    +IK +A    +R + + + +V    + ++ ++Q +   +  +M  V GR+L  P   +G +   +      + + + K  FH  ++   WA+  F  +R+   E  L     +L K +    + I     FC  +                   +             Y  VK +GD  LG+ TQC+  +N+   K   Q + NL LK+N KLGG+N  +   +   +    ++F G DVTHP   DG   S+AAVVGS++   + Y A ++ Q  +      E++ ++  MV+ L+  FY     K P  +IFYRDGVSEGQF  V  +E+ AI+ AC   +K Y+P ITYI+VQKRH+ R       + VGR  N+  G  +D DITH  EFDFYLCSH GIQGTS+P HYYV+ D+N + +++ Q  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:   17 VPRRPGYG-TMGKPIKLLANCFQVDIPKMDVYLYDVDIKPEKCPRRVN----------REVVDSMVQHFKVTIFGDRRPVYDGKKSLYTAQPLPVASAGVDLDVTLPGEGGKDRIFKVTIKFVSLVSWHMLHEVLT----GRSTPDPLELDKPISTNPVHAVDVVLRH-LPSMRYTPVGRSFFSSPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMR-----RKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISR-LVRSANYNSDPFVQEFQFRVRDEMAEVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQ-FHTGVEIKMWAIACFATQRQC-REEVLKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADNVEPMFRHLKNTYAGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVV--KTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQ-----EVIQDLASMVRELLIQFYKSTHYK-PTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKEYQPGITYIVVQKRHHTRLFCADRAERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYYVLWDDNCFTADEFQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVS 832          
BLAST of Protein argonaute-3 vs. Ensembl Zebrafish
Match: ago4 (argonaute RISC component 4 [Source:ZFIN;Acc:ZDB-GENE-030131-5357])

HSP 1 Score: 329.331 bits (843), Expect = 8.479e-97
Identity = 248/847 (29.28%), Postives = 405/847 (47.82%), Query Frame = 3
Query:  477 FLPERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGLPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEI----IEN---LGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQ----KVMGNDPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXXXXXXXXXXXXXXX------XXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQ-----LSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIR-FCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            F P R    GT G+ I L+ N F + +    ++ YDI++ K E       R+  + +      R    Q   D+   P +DG R ++    LP       L      E ++    ++LQ V+    +  +   + +  G  N +P+++++ LD+I R  L       +G      P            +  G+  ++RP M N+  N+D +    ++A  + +++ +V  + N N Q K   +S+       I  +KV+ +H          R + V  V ++ A ++     +EN   +  +V +YF   + + LKYP L  ++     K    P E+  I+  Q    K+  N  + + +    SA    E    L KS     G   + YL+ + I +   M  V GR+L  P   +G + + +      + + + K  F+  I+   WA+  F  +++   E+ L +   +L K +    + I     FC  +              +  + + +           Y  VK +GD  LG+ TQC+  +N+   K   Q + NL LK+NAKLGG+N  +   +   +    ++F G DVTHP   DG   S+AAVVGS++   + Y A ++ Q     LS++   + E++ ++ +MV+ L+  FY   + K P  +I+YR GVSEGQ + V   E+ AI+ AC   +++Y+P ITYI+VQKRH+ R FC    E  VG+  NV  G  +D+ ITH  EFDFYLCSH GIQGTS+P HY+V+ D+N + +++LQ  TY LC+   RCTRS+S PAP +YA L AFR R    D+D+ S    + S
Sbjct:   15 FQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDIDI-KPEKRPRRVNREVVDTM-----VRHFKMQIFGDR--QPGYDGKRNMYTAHPLPIGRDRVDLEVTLPGEGKDQTFKVSLQWVS----VVSLQMLLEALSGHLNEVPEDSVQALDVITRH-LPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPVIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQ-----MKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGG--PDPYLKEFGIVVHNDMTEVTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQ-FYAGIEIKVWAVACFAPQKQC-REDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMSYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVV--KTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQDLSQEQLFSQEVIQDLTNMVRELLIQFYKSTRFK-PTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCSDKAE-RVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVS 835          
BLAST of Protein argonaute-3 vs. Ensembl Zebrafish
Match: ago3b (argonaute RISC catalytic component 3b [Source:NCBI gene;Acc:568159])

HSP 1 Score: 326.25 bits (835), Expect = 1.345e-95
Identity = 238/859 (27.71%), Postives = 393/859 (45.75%), Query Frame = 3
Query:  480 LPERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCG--LPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINC-HLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQE--------TIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDP-----------FHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXXXXXXXXXXXXXX------XXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            +P RP  G T G+ I L+ NCF + +    +++YD+++   +     N          +++   +V  F     G   P++DG + ++  Q LP       L+     E        + ++ V+      L +       GRS   P E         +  +D+++R  L       +G     +P            +  G+  ++RP M  +  N+D +    ++A  +  ++ +V  ++N + Q +   +S        I  +KV+ +H    +     R + V  V ++ A ++         + + ++V +YF   + + LKYP L  ++     K    P E+  I+  Q+ +     N    +IK +A    +R + + + +V    + ++ ++Q +   +  +M  V GR+L  P   +G +      S  + MN+    P           FH  ++   WA+  F  +R+   E  L     +L K +    + I     FC  +                   +             Y  VK +GD  LG+ TQC+  +N+   K   Q + NL LK+N KLGG+N  +   +   +    ++F G DVTHP   DG   S+AAVVGS++   + Y A ++ Q  +      E++ ++  MV+ L+  FY     K P  +IFYRDGVSEGQF  V  +E+ AI+ AC   +K Y+P ITYI+VQKRH+ R       + VGR  N+  G  +D DITH  EFDFYLCSH GIQGTS+P HYYV+ D+N + +++ Q  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:   17 VPRRPGYG-TMGKPIKLLANCFQVDIPKMDVYLYDVDIKPEKCPRRVN----------REVVDSMVQHFKVTIFGDRRPVYDGKKSLYTAQPLPVASAGVDLDVTLPGEGGKDRIFKVTIKFVSLVSWHMLHEVLT----GRSTPDPLELDKPISTNPVHAVDVVLRH-LPSMRYTPVGRSFFSSPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMR-----RKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISR-LVRSANYNSDPFVQEFQFRVRDEMAEVTGRVLPAPMLQYGGR-----VSSEHFMNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQC-REEVLKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADNVEPMFRHLKNTYAGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVV--KTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQ-----EVIQDLASMVRELLIQFYKSTHYK-PTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKEYQPGITYIVVQKRHHTRLFCADRAERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYYVLWDDNCFTADEFQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVS 839          
BLAST of Protein argonaute-3 vs. Ensembl Zebrafish
Match: ago3a (argonaute RISC catalytic component 3a [Source:ZFIN;Acc:ZDB-GENE-051107-16])

HSP 1 Score: 319.316 bits (817), Expect = 3.286e-93
Identity = 234/859 (27.24%), Postives = 394/859 (45.87%), Query Frame = 3
Query:  480 LPERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCG--LPIFDGGRIIFNNQLLPYTETINKLNFIYNYEN-QNINCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQE--------TIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDP-----------FHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXXXXXXXXXXXXXX------XXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVN-WEIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            +P RP  G T G+ I L+ NCF + +    +++Y++++   +     N          +++   +V  F     G   P++DG + ++    LP       L+     E  ++    ++++ V+      L +       GRS   P E         +  +D+++R  L       +G      P            +  G+  ++RP M  +  N+D +    ++A  +  ++ +V  ++N + Q +   +S        I  +KV+ +H    +     R + V  V ++ A ++         + + ++V +YF   + + LKYP L  ++     K    P E+  I+  Q+ +     N    +IK +A    +R + + + +V    +  + ++Q +   +  +M +V GR+L  P   +G +      S  + MN+    P           FH  ++   WA+  F  +R+   E  L     +L K +    + I     FC  +                   +             Y  VK +GD  LG+ TQC+  +N+   K   Q + NL LK+N KLGG+N   + +   +  +   +  G DVTHP   DG   S+AAVVGS++   + Y A ++ Q  +      E++ ++  MV+ L+  FY   + K P  +IFYRDGVSEGQF  V  +E+ AI+ AC   +K Y+P ITYI+VQKRH+ R       + VGR  N+  G  +D DITH  EFDFYLCSH GIQGTS+P HY+V+ D+N + +++ Q  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:   17 MPRRPGYG-TMGKPIKLLANCFQVEIPKMDVYLYEVDIKPEKCPRRVN----------REVVDSMVQHFKVTIFGDRRPVYDGKKSLYTANPLPVAPAGVDLDVTLPGEGGKDRPFKVSIKFVSLVSWHLLHEVLT----GRSMPEPLELDKPISTNPVHAVDVVLRH-LPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMR-----RKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISR-LVRSANYEADPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGR-----VSTEHFMNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQC-REEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVV--KTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQ-----EVIQDLSSMVRELLIQFYKSTRYK-PTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKEYQPGITYIVVQKRHHTRLFCADRNERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADEFQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVS 839          
BLAST of Protein argonaute-3 vs. Ensembl Xenopus
Match: rbm26 (RNA binding motif protein 26 [Source:Xenbase;Acc:XB-GENE-960053])

HSP 1 Score: 349.362 bits (895), Expect = 3.836e-104
Identity = 248/845 (29.35%), Postives = 408/845 (48.28%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCG--LPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSG-KGRSNNIPQETIRVLDIIIREMLLDCDLILIG------NKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXX------XXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            P RPD  GT GR I L  N F M +   +I+ Y+I++   +     N          ++I   +V  F     G   P+FDG + ++    LP      +L      E ++    + ++ +            +H    GR  ++P ETI+ LD+++R  L       +G      ++G  NP    +       +  G+  ++RP +  +  N+D +    ++A  + +++ +V    +   Q K   +S+       I  +KV+ +H          R + V  V ++ A ++         + +  +V +YF   H + L+YP L  ++     K    P E+  I+  Q+ +     N    +I+ +A    +R + + K ++    F  + +++ + I +   M +V GR+L  P+ L+G + + +      + + + K  FH  I+   WA+  F  +R+   E  L     +L K +    + I     FC  +                      ++V++      Y  VK +GD  LG+ TQC+  +N+  Q+   Q + NL LK+N KLGGVN  +      P+    ++F G DVTHP   DG   S+AAVVGS++     Y A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  +IFYRDGVSEGQF+ V +HE+ AI+ AC K +K+Y+P IT+I+VQKRH+ R       + VG+  N+  G  +D  ITH  EFDFYLCSH GIQGTS+P HY+V+ D+NR++S++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:    9 PPRPD-FGTSGRTIKLQANFFEMDIPKIEIYHYEIDIKPEKCPRRVN----------REIVEHMVQHFKAQIFGDRKPVFDGRKNLYTAMPLPIARDKVELEVTLPGEGKDRIFKVAIKWMACVSLQA-----LHDALSGRLPSVPFETIQALDVVMRH-LPSMRYTPVGRSFFTASEGCANPLGGGR------EVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFMCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQ-----MKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISK-LMRSASFNTDPFVREFGIMVKDDMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQ-FHTGIEIKVWAIACFAPQRQC-TEVHLKTFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYTGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNV--QRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQ-----EIIQDLSAMVRELLIQFYKSTRFK-PTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTS 814          
BLAST of Protein argonaute-3 vs. Ensembl Xenopus
Match: rbm26 (RNA binding motif protein 26 [Source:Xenbase;Acc:XB-GENE-960053])

HSP 1 Score: 348.206 bits (892), Expect = 1.241e-103
Identity = 251/854 (29.39%), Postives = 412/854 (48.24%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCG--LPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSG-KGRSNNIPQETIRVLDIIIREMLLDCDLILIG------NKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSK---------FNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXX------XXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            P RPD  GT GR I L  N F M +   +I+ Y+I++   +     N          ++I   +V  F     G   P+FDG + ++    LP      +L      E ++    + ++ +            +H    GR  ++P ETI+ LD+++R  L       +G      ++G  NP    +       +  G+  ++RP +  +  N+D +    ++A  + +++ +V    +   Q K   +S+       I  +KV+ +H          R + V  V ++ A ++         + +  +V +YF   H + L+YP L  ++     K    P E+  I+  Q+ +     N    +I+ +A    +R + + K ++    F  + +++ + I +   M +V GR+L  P+ L+G +  + P +K         +++ NK+F    H  I+   WA+  F  +R+   E  L     +L K +    + I     FC  +                      ++V++      Y  VK +GD  LG+ TQC+  +N+  Q+   Q + NL LK+N KLGGVN  +      P+    ++F G DVTHP   DG   S+AAVVGS++     Y A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  +IFYRDGVSEGQF+ V +HE+ AI+ AC K +K+Y+P IT+I+VQKRH+ R       + VG+  N+  G  +D  ITH  EFDFYLCSH GIQGTS+P HY+V+ D+NR++S++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:   19 PPRPD-FGTSGRTIKLQANFFEMDIPKIEIYHYEIDIKPEKCPRRVN----------REIVEHMVQHFKAQIFGDRKPVFDGRKNLYTAMPLPIARDKVELEVTLPGEGKDRIFKVAIKWMACVSLQA-----LHDALSGRLPSVPFETIQALDVVMRH-LPSMRYTPVGRSFFTASEGCANPLGGGR------EVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFMCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQ-----MKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISK-LMRSASFNTDPFVREFGIMVKDDMTDVTGRVLQPPSILYGGRVWEEPNNKAIATPVQGVWDMRNKQF----HTGIEIKVWAIACFAPQRQC-TEVHLKTFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYTGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNV--QRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQ-----EIIQDLSAMVRELLIQFYKSTRFK-PTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTS 830          
BLAST of Protein argonaute-3 vs. Ensembl Xenopus
Match: rbm26 (RNA binding motif protein 26 [Source:Xenbase;Acc:XB-GENE-960053])

HSP 1 Score: 348.206 bits (892), Expect = 3.456e-103
Identity = 248/845 (29.35%), Postives = 408/845 (48.28%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCG--LPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSG-KGRSNNIPQETIRVLDIIIREMLLDCDLILIG------NKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXX------XXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            P RPD  GT GR I L  N F M +   +I+ Y+I++   +     N          ++I   +V  F     G   P+FDG + ++    LP      +L      E ++    + ++ +            +H    GR  ++P ETI+ LD+++R  L       +G      ++G  NP    +       +  G+  ++RP +  +  N+D +    ++A  + +++ +V    +   Q K   +S+       I  +KV+ +H          R + V  V ++ A ++         + +  +V +YF   H + L+YP L  ++     K    P E+  I+  Q+ +     N    +I+ +A    +R + + K ++    F  + +++ + I +   M +V GR+L  P+ L+G + + +      + + + K  FH  I+   WA+  F  +R+   E  L     +L K +    + I     FC  +                      ++V++      Y  VK +GD  LG+ TQC+  +N+  Q+   Q + NL LK+N KLGGVN  +      P+    ++F G DVTHP   DG   S+AAVVGS++     Y A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  +IFYRDGVSEGQF+ V +HE+ AI+ AC K +K+Y+P IT+I+VQKRH+ R       + VG+  N+  G  +D  ITH  EFDFYLCSH GIQGTS+P HY+V+ D+NR++S++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:   61 PPRPD-FGTSGRTIKLQANFFEMDIPKIEIYHYEIDIKPEKCPRRVN----------REIVEHMVQHFKAQIFGDRKPVFDGRKNLYTAMPLPIARDKVELEVTLPGEGKDRIFKVAIKWMACVSLQA-----LHDALSGRLPSVPFETIQALDVVMRH-LPSMRYTPVGRSFFTASEGCANPLGGGR------EVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFMCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQ-----MKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISK-LMRSASFNTDPFVREFGIMVKDDMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQ-FHTGIEIKVWAIACFAPQRQC-TEVHLKTFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYTGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNV--QRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQ-----EIIQDLSAMVRELLIQFYKSTRFK-PTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTS 866          
BLAST of Protein argonaute-3 vs. Ensembl Xenopus
Match: rbm26 (RNA binding motif protein 26 [Source:Xenbase;Acc:XB-GENE-960053])

HSP 1 Score: 344.739 bits (883), Expect = 1.344e-102
Identity = 244/838 (29.12%), Postives = 404/838 (48.21%), Query Frame = 3
Query:  504 GTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCG--LPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSG-KGRSNNIPQETIRVLDIIIREMLLDCDLILIG------NKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXX------XXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            GT GR I L  N F M +   +I+ Y+I++   +     N          ++I   +V  F     G   P+FDG + ++    LP      +L      E ++    + ++ +            +H    GR  ++P ETI+ LD+++R  L       +G      ++G  NP    +       +  G+  ++RP +  +  N+D +    ++A  + +++ +V    +   Q K   +S+       I  +KV+ +H          R + V  V ++ A ++         + +  +V +YF   H + L+YP L  ++     K    P E+  I+  Q+ +     N    +I+ +A    +R + + K ++    F  + +++ + I +   M +V GR+L  P+ L+G + + +      + + + K  FH  I+   WA+  F  +R+   E  L     +L K +    + I     FC  +                      ++V++      Y  VK +GD  LG+ TQC+  +N+  Q+   Q + NL LK+N KLGGVN  +      P+    ++F G DVTHP   DG   S+AAVVGS++     Y A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  +IFYRDGVSEGQF+ V +HE+ AI+ AC K +K+Y+P IT+I+VQKRH+ R       + VG+  N+  G  +D  ITH  EFDFYLCSH GIQGTS+P HY+V+ D+NR++S++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:    5 GTSGRTIKLQANFFEMDIPKIEIYHYEIDIKPEKCPRRVN----------REIVEHMVQHFKAQIFGDRKPVFDGRKNLYTAMPLPIARDKVELEVTLPGEGKDRIFKVAIKWMACVSLQA-----LHDALSGRLPSVPFETIQALDVVMRH-LPSMRYTPVGRSFFTASEGCANPLGGGR------EVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFMCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQ-----MKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISK-LMRSASFNTDPFVREFGIMVKDDMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQ-FHTGIEIKVWAIACFAPQRQC-TEVHLKTFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYTGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNV--QRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQ-----EIIQDLSAMVRELLIQFYKSTRFK-PTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTS 804          
BLAST of Protein argonaute-3 vs. Ensembl Xenopus
Match: rbm26 (RNA binding motif protein 26 [Source:Xenbase;Acc:XB-GENE-960053])

HSP 1 Score: 343.969 bits (881), Expect = 6.313e-102
Identity = 251/857 (29.29%), Postives = 409/857 (47.72%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCG--LPIFDGGRIIFNNQLLPYTETIN-KLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSG-KGRSNNIPQETIRVLDIIIREMLLDCDLILIG------NKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDP-----------FHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXX------XXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            P RPD  GT GR I L  N F M +   +I+ Y+I++   +     N          ++I   +V  F     G   P+FDG + ++    LP       +L      E ++    + ++ +            +H    GR  ++P ETI+ LD+++R  L       +G      ++G  NP    +       +  G+  ++RP +  +  N+D +    ++A  + +++ +V    +   Q K   +S+       I  +KV+ +H          R + V  V ++ A ++         + +  +V +YF   H + L+YP L  ++     K    P E+  I+  Q+ +     N    +I+ +A    +R + + K ++    F  + +++ + I +   M +V GR+L  P+ L+G +  + P +    +NK    P           FH  I+   WA+  F  +R+   E  L     +L K +    + I     FC  +                      ++V++      Y  VK +GD  LG+ TQC+  +N+  Q+   Q + NL LK+N KLGGVN  +      P+    ++F G DVTHP   DG   S+AAVVGS++     Y A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  +IFYRDGVSEGQF+ V +HE+ AI+ AC K +K+Y+P IT+I+VQKRH+ R       + VG+  N+  G  +D  ITH  EFDFYLCSH GIQGTS+P HY+V+ D+NR++S++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:   28 PPRPD-FGTSGRTIKLQANFFEMDIPKIEIYHYEIDIKPEKCPRRVN----------REIVEHMVQHFKAQIFGDRKPVFDGRKNLYTAMPLPIARDKQVELEVTLPGEGKDRIFKVAIKWMACVSLQA-----LHDALSGRLPSVPFETIQALDVVMRH-LPSMRYTPVGRSFFTASEGCANPLGGGR------EVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFMCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQ-----MKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISK-LMRSASFNTDPFVREFGIMVKDDMTDVTGRVLQPPSILYGGRVWEEPNAP---LNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQC-TEVHLKTFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYTGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNV--QRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQ-----EIIQDLSAMVRELLIQFYKSTRFK-PTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTS 843          
BLAST of Protein argonaute-3 vs. Ensembl Mouse
Match: Ago2 (argonaute RISC catalytic subunit 2 [Source:MGI Symbol;Acc:MGI:2446632])

HSP 1 Score: 352.443 bits (903), Expect = 3.199e-105
Identity = 249/845 (29.47%), Postives = 411/845 (48.64%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCG--LPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSG-KGRSNNIPQETIRVLDIIIREMLLDCDLILIG------NKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXX------XXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            P RPD  GT GR I L  N F M +    I+ Y++++   +     N          ++I   +V  F     G   P+FDG + ++    LP      +L      E ++    ++++ V+           +H    GR  ++P ETI+ LD+++R  L       +G      ++G  NP    +       +  G+  ++RP +  +  N+D +    ++A  + +++ +V    +   Q K   +S+       I  +KV+ +H          R + V  V ++ A ++         + +  +V +YF   H + L+YP L  ++     K    P E+  I+  Q+ +     N    +I+ +A    +R + + K ++    F  + Y++ + I +  +M +V GR+L  P+ L+G + + +      + + + K  FH  I+   WA+  F  +R+   E  L +   +L K +    + I     FC  +                      ++V++      Y  VK +GD  LG+ TQC+  +N+  Q+   Q + NL LK+N KLGGVN  +      P+    ++F G DVTHP   DG   S+AAVVGS++     Y A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  +IFYRDGVSEGQF+ V +HE+ AI+ AC K +K+Y+P IT+I+VQKRH+ R       + VG+  N+  G  +D  ITH  EFDFYLCSH GIQGTS+P HY+V+ D+NR++S++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:   27 PPRPD-FGTTGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVN----------REIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQA-----LHDALSGRLPSVPFETIQALDVVMRH-LPSMRYTPVGRSFFTASEGCSNPLGGGR------EVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQ-----MKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISK-LMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQ-FHTGIEIKVWAIACFAPQRQC-TEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNV--QRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQ-----EIIQDLAAMVRELLIQFYKSTRFK-PTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTS 832          
BLAST of Protein argonaute-3 vs. Ensembl Mouse
Match: Ago3 (argonaute RISC catalytic subunit 3 [Source:MGI Symbol;Acc:MGI:2446634])

HSP 1 Score: 327.791 bits (839), Expect = 3.294e-96
Identity = 234/848 (27.59%), Postives = 398/848 (46.93%), Query Frame = 3
Query:  480 LPERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCG--LPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQN---------INCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXX------XXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            +P RP  G T G+ I L+ NCF + +    +++Y++++   +     N          +++   +V  F     G   P++DG R ++    LP   T   L+     E                ++  L++  +    + + +   K  S N     +  +D+++R  L       +G      P            +  G+  ++RP M  +  N+D +    ++A  +  ++ +V  ++N + Q +   +S        I  +KV+ +H    +     R + V  V ++ A ++         + + ++V +YF   + + LKYP L  ++     K    P E+  I+  Q+ +     N    +IK +A    +R + + + +V    +  + ++Q +   +  +M +V GR+L  P   +G +   +      + + + K  FH  ++   WA+  F  +R+   E  L     +L K +    + I     FC  +                      ++V++      Y  VK +GD  LG+ TQC+  +N+   K   Q + NL LK+N KLGG+N  +   +   +    ++F G DVTHP   DG   S+AAVVGS++   + Y A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  +IFYRDGVSEGQF  V  +E+ AI+ AC   +K+Y+P ITYI+VQKRH+ R       + VGR  N+  G  +D DITH  EFDFYLCSH GIQGTS+P HY+V+ D+N + +++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:   17 VPRRPGYG-TMGKPIKLLANCFQVEIPKIDVYLYEVDIKPDKCPRRVN----------REVVDSMVQHFKVTIFGDRRPVYDGKRSLYTANPLPVATTGVDLDVTLPGEGGKDRPFKVSVKFVSRVSWHLLHEALAGGTLPEPLELDKPVSTN----PVHAVDVVLRH-LPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMR-----RKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYTLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISR-LVRSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQ-FHTGVEIKMWAIACFATQRQC-REEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVI--KTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQ-----EIIQDLASMVRELLIQFYKSTRFK-PTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNFFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVS 832          
BLAST of Protein argonaute-3 vs. Ensembl Mouse
Match: Ago4 (argonaute RISC catalytic subunit 4 [Source:MGI Symbol;Acc:MGI:1924100])

HSP 1 Score: 313.923 bits (803), Expect = 2.998e-91
Identity = 239/847 (28.22%), Postives = 398/847 (46.99%), Query Frame = 3
Query:  477 FLPERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGLPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEI----IEN---LGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQ----KVMGNDPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXXXXXXXXXXXXXXX------XXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKD-----AVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIR-FCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            F P R    GT G+ I L+ N F + +    ++ YD+++ K E       R+  + +      R    Q   D+   P +DG R ++    LP       +      E ++       ++    + +  +   + +  G  N +P ++++ LD+I R  L       +G      P            +  G+  ++RP M N+  N+D +    ++A  + +++ +V  + N N Q K   +S+       I  +KV+ +H          R + V  V ++ A ++     +EN   +  +V +YF   + + LK+P L  ++     K    P E+  I+  Q    K+  N  + + +    SA    E    L KS     G   + YL+ + I +  +M  + GR+L  P   +G + + +      + + + K  F+  I+   WA+  F  +++   E+ L +   +L K +    + I     FC  +              +  + + +           Y  VK +GD  LG+ TQC+  +N+   K   Q + NL LK+NAKLGG+N  +   +   +    ++F G DVTHP   DG   S+AAVVGS++   + Y A ++ Q S+         + E+V ++  M + L+  FY   + K P  +I+YR GVSEGQ + V   E+ AI+ AC   +++Y+P ITYI+VQKRH+ R FC   +E  VG+  NV  G  +D+ +TH  EFDFYLCSH GIQGTS+P HY V+ D+N + +++LQ  TY LC+   RCTRS+S PAP +YA L AFR R    D+D+ S    + S
Sbjct:   13 FQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDVDI-KPEKRPRRVNREVVDTM-----VRHFKMQIFGDR--QPGYDGKRNMYTAHPLPIGRDRIDMEVTLPGEGKD----QTFKVSVQWVSVVSLQLLLEALAGHLNEVPDDSVQALDVITRH-LPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPIIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQ-----MKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKHPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGG--PDPYLKEFGIVVHNEMTELTGRVLPAPMLQYGGRNKTVATPSQGVWDMRGKQ-FYAGIEIKVWAVACFAPQKQC-REDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMTYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVV--KTSPQTLSNLCLKMNAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEITQELLYSQEVVQDLTSMARELLIQFYKSTRFK-PTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCADKME-RVGKSGNVPAGTTVDSTVTHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVS 833          
BLAST of Protein argonaute-3 vs. Ensembl Mouse
Match: Ago1 (argonaute RISC catalytic subunit 1 [Source:MGI Symbol;Acc:MGI:2446630])

HSP 1 Score: 308.145 bits (788), Expect = 2.846e-89
Identity = 229/831 (27.56%), Postives = 393/831 (47.29%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELL--KAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGLPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXX------XXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVN-WEIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNIS 2885
            P RP  G T G+ I L+ N F + +    ++ Y++++   K    +   V ++  +  +  IF D            P++DG + I+    LP              E ++    ++++ +       L +  + SG+     +P E+++ LD+ +R  L       +G      P            +  G+  ++RP M  +  N+D +    ++A  + +++ +V  + N + Q K   +S+       I  +KV+ +H          R + V  V ++ A ++         + +  +V +YF   + + LKYP L  ++     K    P E+  I+  Q+ +     N    +IK +A    +R + + + ++    +  + Y+Q + I +   M  V GR+L  P   +G +   +      + + + K  F++ I+   WA+  F  +++   E  L     +L K +    + I     FC  +                      ++V++      Y  VK +GD  LG+ TQC+  +N+   K   Q + NL LK+N KLGG+N   + +   A  +   +  G DVTHP   DG   S+ AVVGS++   + Y A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  +IFYRDGV EGQ   + ++E+ AI+ AC K +K+Y+P ITYI+VQKRH+ R       + +G+  N+  G  +D +ITH  EFDFYLCSH GIQGTS+P HYYV+ D+NR+ +++LQ  TY LC+   RCTRS+S PAP +YA L AFR R    D+++ S
Sbjct:   24 PRRPGIG-TVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGD----------RKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALV-SGQ---IPVPLESVQALDVAMRH-LASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQ-----MKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISR-LMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQ-FYNGIEIKVWAIACFAPQKQ-CREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVV--KTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQ-----EIIEDLSYMVRELLIQFYKSTRFK-PTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDS 822          
BLAST of Protein argonaute-3 vs. Ensembl Mouse
Match: Ago1 (argonaute RISC catalytic subunit 1 [Source:MGI Symbol;Acc:MGI:2446630])

HSP 1 Score: 271.166 bits (692), Expect = 7.078e-79
Identity = 171/520 (32.88%), Postives = 266/520 (51.15%), Query Frame = 3
Query: 1377 NLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEK-----LP-KSKFNIMNKKFKDPFHDPIKWALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXXXXXXXXXXXXXXX------XXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVN-WEIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNIS 2885
             LKYP L  ++     K    P E+  I+  Q+ +     N    +IK +A    +R + + + ++    +  + Y+Q + I +   M  V GR+L  P   +G +  +      P +  +++  K+F +     + WA+  F  +++   E  L     +L K +    + I     FC  +                   + +           Y  VK +GD  LG+ TQC+  +N+   K   Q + NL LK+N KLGG+N   + +   A  +   +  G DVTHP   DG   S+ AVVGS++   + Y A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  +IFYRDGV EGQ   + ++E+ AI+ AC K +K+Y+P ITYI+VQKRH+ R       + +G+  N+  G  +D +ITH  EFDFYLCSH GIQGTS+P HYYV+ D+NR+ +++LQ  TY LC+   RCTRS+S PAP +YA L AFR R    D+++ S
Sbjct:   10 QLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISR-LMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRVSRNRAIATPNQGVWDMRGKQFYNGIEIKV-WAIACFAPQKQ-CREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVV--KTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQ-----EIIEDLSYMVRELLIQFYKSTRFK-PTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDS 518          
BLAST of Protein argonaute-3 vs. UniProt/SwissProt
Match: sp|O77503|AGO2_RABIT (Protein argonaute-2 (Fragment) OS=Oryctolagus cuniculus OX=9986 GN=AGO2 PE=1 SV=2)

HSP 1 Score: 352.829 bits (904), Expect = 1.134e-104
Identity = 249/845 (29.47%), Postives = 411/845 (48.64%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCG--LPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSG-KGRSNNIPQETIRVLDIIIREMLLDCDLILIG------NKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXX------XXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            P RPD  GT GR I L  N F M +    I+ Y++++   +     N          ++I   +V  F     G   P+FDG + ++    LP      +L      E ++    ++++ V+           +H    GR  ++P ETI+ LD+++R  L       +G      ++G  NP    +       +  G+  ++RP +  +  N+D +    ++A  + +++ +V    +   Q K   +S+       I  +KV+ +H          R + V  V ++ A ++         + +  +V +YF   H + L+YP L  ++     K    P E+  I+  Q+ +     N    +I+ +A    +R + + K ++    F  + Y++ + I +  +M +V GR+L  P+ L+G + + +      + + + K  FH  I+   WA+  F  +R+   E  L +   +L K +    + I     FC  +                      ++V++      Y  VK +GD  LG+ TQC+  +N+  Q+   Q + NL LK+N KLGGVN  +      P+    ++F G DVTHP   DG   S+AAVVGS++     Y A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  +IFYRDGVSEGQF+ V +HE+ AI+ AC K +K+Y+P IT+I+VQKRH+ R       + VG+  N+  G  +D  ITH  EFDFYLCSH GIQGTS+P HY+V+ D+NR++S++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:    7 PPRPD-FGTSGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVN----------REIVEHMVQHFKAQIFGDRKPVFDGRKNLYTAMPLPIGREKVELEVTLPGEGKDRIFKVSIKWVSCVSLQA-----LHDALSGRLPSVPFETIQALDVVMRH-LPSMRYTPVGRSFFTASEGCSNPLGGGR------EVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQ-----MKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISK-LMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQ-FHTGIEIKVWAIACFAPQRQC-TEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVGPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNV--QRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQ-----EIIQDLAAMVRELLIQFYKSTRFK-PTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTS 812          
BLAST of Protein argonaute-3 vs. UniProt/SwissProt
Match: sp|Q6QME8|AGO2_BOVIN (Protein argonaute-2 OS=Bos taurus OX=9913 GN=AGO2 PE=2 SV=1)

HSP 1 Score: 352.443 bits (903), Expect = 1.941e-104
Identity = 249/845 (29.47%), Postives = 411/845 (48.64%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCG--LPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSG-KGRSNNIPQETIRVLDIIIREMLLDCDLILIG------NKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXX------XXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            P RPD  GT GR I L  N F M +    I+ Y++++   +     N          ++I   +V  F     G   P+FDG + ++    LP      +L      E ++    ++++ V+           +H    GR  ++P ETI+ LD+++R  L       +G      ++G  NP    +       +  G+  ++RP +  +  N+D +    ++A  + +++ +V    +   Q K   +S+       I  +KV+ +H          R + V  V ++ A ++         + +  +V +YF   H + L+YP L  ++     K    P E+  I+  Q+ +     N    +I+ +A    +R + + K ++    F  + Y++ + I +  +M +V GR+L  P+ L+G + + +      + + + K  FH  I+   WA+  F  +R+   E  L +   +L K +    + I     FC  +                      ++V++      Y  VK +GD  LG+ TQC+  +N+  Q+   Q + NL LK+N KLGGVN  +      P+    ++F G DVTHP   DG   S+AAVVGS++     Y A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  +IFYRDGVSEGQF+ V +HE+ AI+ AC K +K+Y+P IT+I+VQKRH+ R       + VG+  N+  G  +D  ITH  EFDFYLCSH GIQGTS+P HY+V+ D+NR++S++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:   27 PPRPD-FGTSGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVN----------REIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQA-----LHDALSGRLPSVPFETIQALDVVMRH-LPSMRYTPVGRSFFTASEGCSNPLGGGR------EVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQ-----MKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISK-LMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQ-FHTGIEIKVWAIACFAPQRQC-TEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNV--QRTTPQTLSNLWLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQ-----EIIQDLAAMVRELLIQFYKSTRFK-PTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTS 832          
BLAST of Protein argonaute-3 vs. UniProt/SwissProt
Match: sp|Q8CJG0|AGO2_MOUSE (Protein argonaute-2 OS=Mus musculus OX=10090 GN=Ago2 PE=1 SV=3)

HSP 1 Score: 352.443 bits (903), Expect = 2.237e-104
Identity = 249/845 (29.47%), Postives = 411/845 (48.64%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCG--LPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSG-KGRSNNIPQETIRVLDIIIREMLLDCDLILIG------NKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXX------XXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            P RPD  GT GR I L  N F M +    I+ Y++++   +     N          ++I   +V  F     G   P+FDG + ++    LP      +L      E ++    ++++ V+           +H    GR  ++P ETI+ LD+++R  L       +G      ++G  NP    +       +  G+  ++RP +  +  N+D +    ++A  + +++ +V    +   Q K   +S+       I  +KV+ +H          R + V  V ++ A ++         + +  +V +YF   H + L+YP L  ++     K    P E+  I+  Q+ +     N    +I+ +A    +R + + K ++    F  + Y++ + I +  +M +V GR+L  P+ L+G + + +      + + + K  FH  I+   WA+  F  +R+   E  L +   +L K +    + I     FC  +                      ++V++      Y  VK +GD  LG+ TQC+  +N+  Q+   Q + NL LK+N KLGGVN  +      P+    ++F G DVTHP   DG   S+AAVVGS++     Y A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  +IFYRDGVSEGQF+ V +HE+ AI+ AC K +K+Y+P IT+I+VQKRH+ R       + VG+  N+  G  +D  ITH  EFDFYLCSH GIQGTS+P HY+V+ D+NR++S++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:   27 PPRPD-FGTTGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVN----------REIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQA-----LHDALSGRLPSVPFETIQALDVVMRH-LPSMRYTPVGRSFFTASEGCSNPLGGGR------EVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQ-----MKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISK-LMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQ-FHTGIEIKVWAIACFAPQRQC-TEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNV--QRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQ-----EIIQDLAAMVRELLIQFYKSTRFK-PTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTS 832          
BLAST of Protein argonaute-3 vs. UniProt/SwissProt
Match: sp|Q9UKV8|AGO2_HUMAN (Protein argonaute-2 OS=Homo sapiens OX=9606 GN=AGO2 PE=1 SV=3)

HSP 1 Score: 352.443 bits (903), Expect = 2.398e-104
Identity = 249/845 (29.47%), Postives = 411/845 (48.64%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCG--LPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSG-KGRSNNIPQETIRVLDIIIREMLLDCDLILIG------NKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXX------XXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            P RPD  GT GR I L  N F M +    I+ Y++++   +     N          ++I   +V  F     G   P+FDG + ++    LP      +L      E ++    ++++ V+           +H    GR  ++P ETI+ LD+++R  L       +G      ++G  NP    +       +  G+  ++RP +  +  N+D +    ++A  + +++ +V    +   Q K   +S+       I  +KV+ +H          R + V  V ++ A ++         + +  +V +YF   H + L+YP L  ++     K    P E+  I+  Q+ +     N    +I+ +A    +R + + K ++    F  + Y++ + I +  +M +V GR+L  P+ L+G + + +      + + + K  FH  I+   WA+  F  +R+   E  L +   +L K +    + I     FC  +                      ++V++      Y  VK +GD  LG+ TQC+  +N+  Q+   Q + NL LK+N KLGGVN  +      P+    ++F G DVTHP   DG   S+AAVVGS++     Y A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  +IFYRDGVSEGQF+ V +HE+ AI+ AC K +K+Y+P IT+I+VQKRH+ R       + VG+  N+  G  +D  ITH  EFDFYLCSH GIQGTS+P HY+V+ D+NR++S++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:   26 PPRPD-FGTSGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVN----------REIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQA-----LHDALSGRLPSVPFETIQALDVVMRH-LPSMRYTPVGRSFFTASEGCSNPLGGGR------EVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQ-----MKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISK-LMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQ-FHTGIEIKVWAIACFAPQRQC-TEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNV--QRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQ-----EIIQDLAAMVRELLIQFYKSTRFK-PTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTS 831          
BLAST of Protein argonaute-3 vs. UniProt/SwissProt
Match: sp|Q9QZ81|AGO2_RAT (Protein argonaute-2 OS=Rattus norvegicus OX=10116 GN=Ago2 PE=1 SV=2)

HSP 1 Score: 352.058 bits (902), Expect = 3.127e-104
Identity = 249/845 (29.47%), Postives = 410/845 (48.52%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCG--LPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSG-KGRSNNIPQETIRVLDIIIREMLLDCDLILIG------NKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXX------XXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            P RPD  GT GR I L  N F M +    I+ Y++++   +     N          ++I   +V  F     G   P+FDG + ++    LP      +L      E ++    ++++ V+           +H    GR  ++P ETI+ LD+++R  L       +G      ++G  NP    +       +  G+  ++RP +  +  N+D +    ++A  + +++ +V    +   Q K   +S+       I  +KV+ +H          R + V  V ++ A ++         + +  +V +YF   H + L+YP L  ++     K    P E+  I+  Q+ +     N    +I+ +A    +R + + K ++    F  + Y++ + I +  +M +V GR+L  P+ L+G + + +      + + + K  FH  I+   WA+  F  +R+   E  L +   +L K +    + I     FC  +                      ++V++      Y  VK +GD  LG+ TQC+  +N+  Q+   Q + NL LK+N KLGGVN  +      P+    ++F G DVTHP   DG   S+AAVVGS++     Y A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  +IFYRDGVSEGQF+ V +HE+ AI+ AC K +K Y+P IT+I+VQKRH+ R       + VG+  N+  G  +D  ITH  EFDFYLCSH GIQGTS+P HY+V+ D+NR++S++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:   27 PPRPD-FGTTGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVN----------REIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQA-----LHDALSGRLPSVPFETIQALDVVMRH-LPSMRYTPVGRSFFTASEGCSNPLGGGR------EVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQ-----MKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISK-LMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQ-FHTGIEIKVWAIACFAPQRQC-TEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNV--QRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQ-----EIIQDLAAMVRELLIQFYKSTRFK-PTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKEYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTS 832          
BLAST of Protein argonaute-3 vs. TrEMBL
Match: A0A0X3NYE9 (Protein argonaute-3 (Fragment) OS=Schistocephalus solidus OX=70667 GN=AGO3 PE=4 SV=1)

HSP 1 Score: 474.167 bits (1219), Expect = 3.747e-146
Identity = 314/848 (37.03%), Postives = 464/848 (54.72%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGLPIFDGGRIIFNNQLLP-YTET-INKLNFIYNYENQN-INCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSRIYNIK---VKASHYKNDQGENYTRIFTVKGVYQKNAHNEIIENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK--FPAELLTILPNQKVMGNDPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIKWALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFC--YVSNXXXXXXXXXXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVFGVDVTHPS--NNDGLSKSVAAVVGSINKTATIYSAVIKKQ--LSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENESIGLPD--ISINEF--HQNK---MFFI 2963
            P RPD GGT G  + + +NC++MT+    + MYDIE L    + +N   +   ++  KD+ R ++   +K      ++DGGRII+    LP  TE  I+K   + +  +++ +     ++LV      DL   Y+ + +  +  +PQE+IR++D I + +  D      G   +F P   ++L    + I  G+ +++RP    +R N+D T        N+ D +   +     +I+N    NS + +IK   V+A  YKN  G++++R FTV  +   +A   IIE+  +SV EYF   H I LKYP+L  ++ +N      P ELL +LP Q       ++  E+I+ +A+   ERF  L + V  F   + +   + Y I +  Q   V  R+L +P A FG ++ +L + K+ I N   +    +P+KWA+            N+   ++  LP+AA +   +      FC     ++      +   + + L+V+V+ ++  Y  +K I DI+LGI TQC+    L +         NLMLK+N KLGG+NW + N    PIK   MVFG DVTHP+   ND L KSVAAV+GSI      Y+AV+++Q    +  K   EI+  ME++V  L+K +Y  N  + PD LIFYRDGVSEGQF+IV   E+ AIQ AC   +  Y+P ITYI+VQKRH+IRF P+       R KNV PG V+D  ITH REFDFYLCS EGIQGTSKP HY+V+ D++ W S+ LQ FTYFLC+   RC RS+SYPAPT+Y+HLAAFR R          L++ENE    PD  I+ N F  H ++   MFF+
Sbjct:  155 PCRPDNGGTVGESVLIKLNCWDMTIADRTVLMYDIEALGVYRMKDNEKVEV--RLLPKDM-RALLKHIVKRMPSDTVYDGGRIIYKLNPLPGVTEMGIHKEEPVPDPTDRDELVLKYRIKLVQETSTGDLAR-YVENPRASTLAMPQESIRMIDCIFKSINED-KFFSSGRAAIFYPTPIRKLADKLFDIHMGFITSVRPQW-KVRVNVDMTCKAFFPPGNLADVLYYKY---GDDIENPACWNSVLADIKRLRVEARFYKNQDGKSFSRRFTVFSLSTTSAQRLIIEDKNESVFEYFKEHHNITLKYPDLPCVKVSNKRDVFIPMELLHVLPFQNPTARKADVASEIIRCAAVKPQERFRELDRYVGDFM-RQQHALFKHYEINVQTQRGTVSARVLPQPWASFGGRDVELGRGKW-IANTFLQPATVEPLKWAVVNIPPHNA-AQRNQAQLVQ-RLPQAATQFGFR-FDPRPFCKTITPSELEGLMRQLHSQRVTLVVLVLYDNFTYPSIKVISDIKLGIRTQCVKGVTLTK----PNVFPNLMLKINGKLGGINWRVSNY---PIKEAVMVFGADVTHPAPTQNDELRKSVAAVLGSITPDLMRYAAVVRRQDTTERGNKTTREIIDNMEEIVSDLLKAYYVNNNDRFPDRLIFYRDGVSEGQFDIVLTGELPAIQRACAGLRAGYEPAITYIVVQKRHHIRFEPLDP-----RAKNVAPGTVVDTVITHRREFDFYLCSQEGIQGTSKPAHYHVLYDDSNWTSDSLQMFTYFLCHAYMRCPRSVSYPAPTYYSHLAAFRARDW--------LAAENEQ---PDNFITKNRFKVHDSQFTGMFFL 965          
BLAST of Protein argonaute-3 vs. TrEMBL
Match: A0A183TST3 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 PE=4 SV=1)

HSP 1 Score: 466.077 bits (1198), Expect = 3.219e-144
Identity = 317/867 (36.56%), Postives = 467/867 (53.86%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELL---------KAEL-VLENNVRDFTNKIKRKDIF------RDVVSQFLKDKC--GLP---IFDGGRIIFNNQLLP-YTET-INKLNFIYNYENQN-INCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSRIYNIK---VKASHYKNDQGENYTRIFTVKGVYQKNAHNEIIENLGKSVNEYFLSTHGINLKYPELLLIETTNYNKFPAELLTILPNQKVMGNDPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIKWALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFC--YVSNXXXXXXXXXXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVFGVDVTHPS--NNDGLSKSVAAVVGSINKTATIYSAVIKKQ--LSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENESIGLPD--ISINEF--HQNK---MFFI 2963
            P RPD GGT G  + + +NC++MT+    + MYDIE L         K E+ +L  ++R     I ++  +        VV+ +L +     +P   ++DGGRII+    LP  TE  I+K   + +  +++ +     ++LV      DL   Y+ + +  +  +PQE+IR++D I + +  D      G   +F P   ++L    + I  G+ +++RP    +R N+D T        N+ D +   +     +I+N    NS + +IK   V+A  YKN  G++++R FTV  +   +A   IIE+  +SV EYF   H I LKYP+L  +        P ELL +LP Q       ++  E+I+ +A+   ERF  L + V  F   + +   + Y I +  Q   V  R+L +P A FG ++ +L + K+ I N   +    +P+KWA+            N+   ++  LP+AA +   +      FC     ++      +   + + L+V+V+ ++  Y  +K I DI+LGI TQC+    L +         NLMLK+N KLGG+NW + N    PIK   MVFG DVTHP+   ND L KSVAAV+GSI      Y+AV+++Q    +  K   EI+  ME++V  L+K +Y  N  + PD LIFYRDGVSEGQF+IV   E+ AIQ AC   +  Y+P ITYI+VQKRH+IRF P+       R KNV PG V+D  ITH REFDFYLCS EGIQGTSKP HY+V+ D++ W S+ LQ FTYFLC+   RC RS+SYPAPT+Y+HLAAFR R          L++ENE    PD  I+ N F  H ++   MFF+
Sbjct:   34 PCRPDNGGTVGESVLIKLNCWDMTIADRTVLMYDIEALGVYRMKDNEKVEVRLLPKDMRALLKHIVKRYSYLFMQNSMSVVNIYLMNGLLFRMPSDTVYDGGRIIYKLNPLPGVTEMGIHKEEPVPDPTDRDELVLKYRIKLVQETSTGDLAR-YVENPRASTLAMPQESIRMIDCIFKSINED-KFFSSGRAAIFYPTPIRKLADKLFDIHMGFITSVRPQW-KVRVNVDMTCKAFFPPGNLADVLYYKY---GDDIENPACWNSVLADIKRLRVEARFYKNQDGKSFSRRFTVFSLSTTSAQRLIIEDKNESVFEYFKEHHNITLKYPDLPCVSNKRDVFIPMELLHVLPFQNPTARKADVASEIIRCAAVKPQERFRELDRYVGDFMR-QQHALFKHYEINVQTQRGTVSARVLPQPWASFGGRDVELGRGKW-IANTFLQPATVEPLKWAVVNIPPHNA-AQRNQAQLVQ-RLPQAATQFGFR-FDPRPFCKTITPSELEGLMRQLHSQRVTLVVLVLYDNFTYPSIKVISDIKLGIRTQCVKGVTLTK----PNVFPNLMLKINGKLGGINWRVSNY---PIKEAVMVFGADVTHPAPTQNDELRKSVAAVLGSITPDLMRYAAVVRRQDTTERGNKTTREIIDNMEEIVSDLLKAYYVNNNDRFPDRLIFYRDGVSEGQFDIVLTGELPAIQRACAGLRAGYEPAITYIVVQKRHHIRFEPLD-----PRAKNVAPGTVVDTVITHRREFDFYLCSQEGIQGTSKPAHYHVLYDDSNWTSDSLQMFTYFLCHAYMRCPRSVSYPAPTYYSHLAAFRARDW--------LAAENEQ---PDNFITKNRFKVHDSQFTGMFFL 866          
BLAST of Protein argonaute-3 vs. TrEMBL
Match: A0A3P7DMT7 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 GN=SSLN_LOCUS19531 PE=4 SV=1)

HSP 1 Score: 465.692 bits (1197), Expect = 3.729e-144
Identity = 317/867 (36.56%), Postives = 467/867 (53.86%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELL---------KAEL-VLENNVRDFTNKIKRKDIF------RDVVSQFLKDKC--GLP---IFDGGRIIFNNQLLP-YTET-INKLNFIYNYENQN-INCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSRIYNIK---VKASHYKNDQGENYTRIFTVKGVYQKNAHNEIIENLGKSVNEYFLSTHGINLKYPELLLIETTNYNKFPAELLTILPNQKVMGNDPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIKWALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFC--YVSNXXXXXXXXXXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVFGVDVTHPS--NNDGLSKSVAAVVGSINKTATIYSAVIKKQ--LSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENESIGLPD--ISINEF--HQNK---MFFI 2963
            P RPD GGT G  + + +NC++MT+    + MYDIE L         K E+ +L  ++R     I ++  +        VV+ +L +     +P   ++DGGRII+    LP  TE  I+K   + +  +++ +     ++LV      DL   Y+ + +  +  +PQE+IR++D I + +  D      G   +F P   ++L    + I  G+ +++RP    +R N+D T        N+ D +   +     +I+N    NS + +IK   V+A  YKN  G++++R FTV  +   +A   IIE+  +SV EYF   H I LKYP+L  +        P ELL +LP Q       ++  E+I+ +A+   ERF  L + V  F   + +   + Y I +  Q   V  R+L +P A FG ++ +L + K+ I N   +    +P+KWA+            N+   ++  LP+AA +   +      FC     ++      +   + + L+V+V+ ++  Y  +K I DI+LGI TQC+    L +         NLMLK+N KLGG+NW + N    PIK   MVFG DVTHP+   ND L KSVAAV+GSI      Y+AV+++Q    +  K   EI+  ME++V  L+K +Y  N  + PD LIFYRDGVSEGQF+IV   E+ AIQ AC   +  Y+P ITYI+VQKRH+IRF P+       R KNV PG V+D  ITH REFDFYLCS EGIQGTSKP HY+V+ D++ W S+ LQ FTYFLC+   RC RS+SYPAPT+Y+HLAAFR R          L++ENE    PD  I+ N F  H ++   MFF+
Sbjct:   34 PCRPDNGGTVGESVLIKLNCWDMTIADRTVLMYDIEALGVYRMKDNEKVEVRLLPKDMRALLKHIVKRYSYLFMQNSMSVVNIYLMNGLLFRMPSDTVYDGGRIIYKLNPLPGVTEMGIHKEEPVPDPTDRDELVLKYRIKLVQETSTGDLAR-YVENPRASTLAMPQESIRMIDCIFKSINED-KFFSSGRAAIFYPTPIRKLADKLFDIHMGFITSVRPQW-KVRVNVDMTCKAFFPPGNLADVLYYKY---GDDIENPACWNSVLADIKRLRVEARFYKNQDGKSFSRRFTVFSLSTTSAQRLIIEDKNESVFEYFKEHHNITLKYPDLPCVSNKRDVFIPMELLHVLPFQNPTARKADVASEIIRCAAVKPQERFRELDRYVGDFMR-QQHALFKHYEINVQTQRGTVSARVLPQPWASFGGRDVELGRGKW-IANTFLQPATVEPLKWAVVNIPPHNA-AQRNQAQLVQ-RLPQAATQFGFR-FDPRPFCKTITPSELEGLMRQLHSQRVTLVVLVLYDNFTYPSIKVISDIKLGIRTQCVKGVTLTK----PNVFPNLMLKINGKLGGINWRVSNY---PIKEAVMVFGADVTHPAPTQNDELRKSVAAVLGSITPDLMRYAAVVRRQDTTERGNKTTREIIDNMEEIVSDLLKAYYVNNNDRFPDRLIFYRDGVSEGQFDIVLTGELPAIQRACAGLRAGYEPAITYIVVQKRHHIRFEPLD-----PRAKNVAPGTVVDTVITHRREFDFYLCSQEGIQGTSKPAHYHVLYDDSNWTSDSLQMFTYFLCHAYMRCPRSVSYPAPTYYSHLAAFRARDW--------LAAENEQ---PDNFITKNRFKVHDSQFTGMFFL 866          
BLAST of Protein argonaute-3 vs. TrEMBL
Match: A0A3P6Q1W2 (Uncharacterized protein OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS521 PE=4 SV=1)

HSP 1 Score: 461.07 bits (1185), Expect = 3.921e-142
Identity = 299/825 (36.24%), Postives = 446/825 (54.06%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENN---VRDFTNKIKR--KDIFRDVVSQFLKDKCG-------------LP---IFDGGRIIFN-NQLLPYTET-INKLNFIYNYENQN-INCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSRIYNIK---VKASHYKNDQGENYTRIFTVKGVYQKNAHNEIIENLGKSVNEYFLSTHGINLKYPELLLIETTNYNKFPAELLTILPNQKVMGNDPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRN-NKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIKWALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFC--YVSNXXXXXXXXXXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVFGVDVTHPS--NNDGLSKSVAAVVGSINKTATIYSAVIKKQ--LSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGR 2855
            P RPD GGT G  + + +NC++MT+    + MYDIE L    + +N    VR     ++   K I +   S F+ +                +P   ++DGGRI++  ++L    E  I K   + +  +++ +     ++LV      DL   Y+ + +  +  +PQE+IR++D I + +  D   +  G   +F P   K+L    + I  G+ +++RP    +R N+D T        N+ D +   +     +I+N    NS + +IK   V+A  YKN+ G++++R FTV  +   +A   IIE+  +SV EYF   H I L+YP L  +        P ELL +LP Q       ++  E+I+ +A+   ERF  L + V  F   RN +  L+ Y+I +  Q   V  R+L +P A FG ++ +L + K+ + N   +    +P+KWA+            N+   ++  LP+AA +   +      FC     ++      +   + + L+V+V+ ++  Y  +K + DI+LGI TQC+    L +         NLMLK+N KLGG+NW + N    PIK   MVFG DVTHP+   ND L KSVAAV+GSI      Y+AV+++Q    +  K   EI+  ME++V  L+K ++  N  + PD LIFYRDGVSEGQF+ V   E+ AIQ AC   +  Y+P ITYI+VQKRH+IRF P+       R KNV PG V+D  ITH REFDFYLCS EGIQGTSKP HY+V+ D++ W S+ LQ FTYFLC+   RC RS+SYPAPT+Y+HLAAFR R
Sbjct:   46 PCRPDNGGTVGDTVLIRLNCWDMTIADRTVLMYDIEALGVYRMKDNEKVEVRLLPKDMRALVKHIVKRYASLFMLNYISDVRNNLLNVMPFRMPSDTVYDGGRIVYTLDKLAGVNEMGIQKEEPVPDPTDRDELVLKYRIKLVQETSTGDLAR-YVENPRASTLAMPQESIRMIDCIFKSINED-KFVSSGRAAIFYPTPIKRLADKLFDIHMGFITSVRPQW-KVRVNVDMTCKAFFPPGNLADVL---YAKYGDDIENPACWNSVLADIKRLRVEAKFYKNEDGKSFSRRFTVFSLSTTSAQRLIIEDKNQSVFEYFKEHHKITLEYPGLPCVSNKRDVFIPMELLHVLPYQNPTARKADVASEIIRCAAVKPQERFRELDRYVSEF--MRNQHALLKRYDINVQTQRGTVSARVLPQPWASFGSRDVELGRGKW-LFNTFLQPATAEPLKWAVVNIPPHNA-AQRNQATLVQ-RLPQAATQFGFR-FDPRPFCKTITPSELENLMRQLQSQRVTLVVLVLYDNFTYGSIKVLSDIKLGIRTQCVKGVTLTK----PNVFPNLMLKINGKLGGINWSVSNY---PIKESVMVFGADVTHPAPTQNDELRKSVAAVLGSITPDLMRYAAVVRRQDTTERGNKTTREIIDNMEEIVSDLLKAYFVNNNDRFPDRLIFYRDGVSEGQFDTVLTGELPAIQKACAGLRAGYEPAITYIVVQKRHHIRFEPLD-----PRAKNVAPGTVVDTVITHRREFDFYLCSQEGIQGTSKPAHYHVLYDDSNWTSDALQMFTYFLCHAYMRCPRSVSYPAPTYYSHLAAFRAR 846          
BLAST of Protein argonaute-3 vs. TrEMBL
Match: A0A068WVW1 (Eukaryotic translation initiation factor 2c OS=Echinococcus granulosus OX=6210 GN=EgrG_000911700 PE=4 SV=1)

HSP 1 Score: 441.425 bits (1134), Expect = 1.170e-135
Identity = 291/854 (34.07%), Postives = 453/854 (53.04%), Query Frame = 3
Query:  477 FLPERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGLPIFDGGRIIF--------NNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSRIYNIKVKASHYKND-QGENYTRIFTVKGVYQKNAHNEIIENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK--FPAELLTILPNQKVMGNDPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQM---------INVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIKWALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVS-NXXXXXXXXXXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVFGVDVTHPSNNDG---LSKSVAAVVGSINKTATIYSAVIKKQLS-KDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENESIGLPDISINEFHQNKMFFI 2963
             LP RP  GGT GR+I + VNC++  V    + MYDI L K   +L  + ++   K+K K I +  V    + K G    DGGRI+F        + ++L ++E I         +N ++      + V S     + + Y+ + + +++++PQ  I +LD +I+  +    L  +    +F  R  +      + I  GY  + RP     R N+D        A N+ D +   +  N ++    KH+   I ++ V+ASHYKN+  G+ Y + F V G+   +A  E+I ++ KS+ +YF   +GI+LKYPEL  ++T        P ELL +LP Q    +   I   +I+ +A+   +RF NL++ V +           ++  ++I ++         I V+ R L +P+A F     +L +  +N      ++PFH P+  +L         G    +  +  +LP+AA +  V +    +    S  +  K   +  KE + L + V+    EY  +K  G++   + TQC+  +   R+ N+     NLMLK+NAKLGG+NW ++++       L MV G DVTHP    G    +KSVAAVV SI+     Y A++++Q   ++GK   E +  ME +   L+K F   N  +LP  +I YRDGVSEGQF  + + E++A+Q AC   + +Y+P IT+I+VQKRH+IRF P+       R KNVLPG V+D  ITH REFDFYLCSHEGIQGTSKP HY+V+ D+N W S+DLQ+FTY+LC+   RC RS+SYPAPT+Y+HLAAFR R      +N ++  +N        +I+   Q++MFF+
Sbjct:    1 MLPSRPG-GGTIGRKIVVEVNCWDFDVSDVSVLMYDITLTK---LLSTDGKEI--KLKEKGIGK-YVRLIAERKRGDVFHDGGRILFSLGPLDGKDGEMLNFSEKIAD-----PLQNDDLTIEYAAKRVGSISARQIRE-YLDNSRSKTSDLPQPAINMLDNLIK-WVNRASLPYLSKSAIFYDRPERDNTGGLFWIYRGYSLSFRPQW-KCRLNIDMAHRAFFPAGNLADILYAKYGDNMYSPSTWKHVKEDILSLHVEASHYKNEASGKTYRKRFVVHGLSHNSADKEMIADINKSIAKYFKERYGIDLKYPELPCVKTKKDRDEYMPMELLEVLPFQNAKEDPGVIASAIIRCAAVRPADRFGNLREFVRSM----------NWRASLISKLRLGLRDMNPIKVEARELPQPSAHFSTGTVELGRGSWN------QEPFHQPVPRSLRCVVVTMVPGYTRNVNLLMDKLPQAAQRFGVHMEMRREVVKSSIANLPKAFEDLQKEGVKLAIFVLTGTNEYPHIKRQGELHSSMFTQCV-KDGTIRKPNVCN---NLMLKINAKLGGINWVVEDLCRRRSDELLMVVGADVTHPGPTGGGRSFTKSVAAVVASISCDLMRYVAIVRQQDQIREGKSTREDIDGMEGIFSDLLKTFAKNNNDRLPTKVIVYRDGVSEGQFNRILDIELSAMQRACSNLRPDYEPGITFIVVQKRHHIRFNPVG-----SRAKNVLPGTVVDAQITHHREFDFYLCSHEGIQGTSKPAHYHVLYDDNDWQSDDLQQFTYYLCHAYMRCCRSVSYPAPTYYSHLAAFRARNWLKFVENETIIRDNR------FTIHPGQQDQMFFL 808          
BLAST of Protein argonaute-3 vs. Ensembl Cavefish
Match: ago2 (argonaute RISC catalytic component 2 [Source:NCBI gene;Acc:103030212])

HSP 1 Score: 344.739 bits (883), Expect = 1.625e-102
Identity = 249/851 (29.26%), Postives = 412/851 (48.41%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCG--LPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSG-KGRSNNIPQETIRVLDIIIREMLLDCDLILIG------NKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXX------XXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-------GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            P+RPD  GT GR I L  N F M +   +++ YDI++   +     N          ++I   +V  F     G   P++DG + ++    LP      +L      E ++ +  + ++ V+           +H    GR  +IP ETI+ LD+++R  L       +G      ++G  NP    +       +  G+  ++RP +  +  N+D +    ++A  + +++ +V    +   Q K   +S+       I  +KV+ +H          R + V  V ++ A ++         + +  +V +YF   + + L+YP L  ++     K    P E+  I+  Q+ +     N    +I+ +A    +R D + K ++    F  + Y++ + + +  +M  V GR+L  P+ L+G + + +      + + + K  FH  I+   WA+  F  +R+   E  L A   +L K +    + I     FC  +                 +    ++V++      Y  VK +GD  LG+ TQC+  +N+  QK   Q + NL LK+N KLGGVN    NI     +G  +VF       G DVTHP   DG   S+AAVVGS++   + Y A ++ Q  +      +I+ ++  MV+ L+  FY   + K P  +I+YRDG+SEGQF  V  HE+ AI+ AC K +K+Y+P IT+++VQKRH+ R   +   + VG+  N+  G  +D  ITH  EFDFYLCSH GIQGTS+P HY+V+ D+N + S++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:   23 PQRPD-FGTMGRTIKLQANFFEMEIPKLEMYHYDIDIKPEKCPRRVN----------REIVEHMVQHFKTQIFGDRKPVYDGRKNLYTAMPLPIGRDKVELEVTIPGEGKDRSFKVAIKWVSCVSLQA-----LHEALSGRLPSIPFETIQALDVVMRH-LPSMRYTPVGRSFFTPSEGCSNPLGGGR------EVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFMCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQ-----MKRKYRVCNVTRRPASHQTFPLQQENGQTIECTVAQYFKDKYKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQDEISK-LMRSANFNTDPYVREFGVMVRDEMTEVNGRVLQAPSILYGGRNKAIATPIQGVWDMRNKQ-FHTGIEIKVWAIACFAPQRQC-TELLLKAFTDQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFKHLKYTYQGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQVKNV--QKTTPQTLSNLCLKINVKLGGVN----NILLP--QGRPLVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQQHRQ-----DIIQDLAAMVRELLIQFYKSTRFK-PTRIIYYRDGISEGQFNQVLQHELLAIREACIKLEKDYQPGITFVVVQKRHHTRLFCMDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNHFTSDELQVLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTS 828          
BLAST of Protein argonaute-3 vs. Ensembl Cavefish
Match: ago2 (argonaute RISC catalytic component 2 [Source:NCBI gene;Acc:103030212])

HSP 1 Score: 345.125 bits (884), Expect = 1.693e-102
Identity = 249/851 (29.26%), Postives = 412/851 (48.41%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCG--LPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSG-KGRSNNIPQETIRVLDIIIREMLLDCDLILIG------NKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXX------XXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-------GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            P+RPD  GT GR I L  N F M +   +++ YDI++   +     N          ++I   +V  F     G   P++DG + ++    LP      +L      E ++ +  + ++ V+           +H    GR  +IP ETI+ LD+++R  L       +G      ++G  NP    +       +  G+  ++RP +  +  N+D +    ++A  + +++ +V    +   Q K   +S+       I  +KV+ +H          R + V  V ++ A ++         + +  +V +YF   + + L+YP L  ++     K    P E+  I+  Q+ +     N    +I+ +A    +R D + K ++    F  + Y++ + + +  +M  V GR+L  P+ L+G + + +      + + + K  FH  I+   WA+  F  +R+   E  L A   +L K +    + I     FC  +                 +    ++V++      Y  VK +GD  LG+ TQC+  +N+  QK   Q + NL LK+N KLGGVN    NI     +G  +VF       G DVTHP   DG   S+AAVVGS++   + Y A ++ Q  +      +I+ ++  MV+ L+  FY   + K P  +I+YRDG+SEGQF  V  HE+ AI+ AC K +K+Y+P IT+++VQKRH+ R   +   + VG+  N+  G  +D  ITH  EFDFYLCSH GIQGTS+P HY+V+ D+N + S++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:   39 PQRPD-FGTMGRTIKLQANFFEMEIPKLEMYHYDIDIKPEKCPRRVN----------REIVEHMVQHFKTQIFGDRKPVYDGRKNLYTAMPLPIGRDKVELEVTIPGEGKDRSFKVAIKWVSCVSLQA-----LHEALSGRLPSIPFETIQALDVVMRH-LPSMRYTPVGRSFFTPSEGCSNPLGGGR------EVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFMCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQ-----MKRKYRVCNVTRRPASHQTFPLQQENGQTIECTVAQYFKDKYKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQDEISK-LMRSANFNTDPYVREFGVMVRDEMTEVNGRVLQAPSILYGGRNKAIATPIQGVWDMRNKQ-FHTGIEIKVWAIACFAPQRQC-TELLLKAFTDQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFKHLKYTYQGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQVKNV--QKTTPQTLSNLCLKINVKLGGVN----NILLP--QGRPLVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQQHRQ-----DIIQDLAAMVRELLIQFYKSTRFK-PTRIIYYRDGISEGQFNQVLQHELLAIREACIKLEKDYQPGITFVVVQKRHHTRLFCMDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNHFTSDELQVLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTS 844          
BLAST of Protein argonaute-3 vs. Ensembl Cavefish
Match: ago4 (protein argonaute-4 [Source:NCBI gene;Acc:103043810])

HSP 1 Score: 323.168 bits (827), Expect = 1.530e-94
Identity = 250/858 (29.14%), Postives = 405/858 (47.20%), Query Frame = 3
Query:  477 FLPERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGLPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEI----IEN---LGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQ----KVMGNDPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFG-------------DKEEKLP-KSKFNIMNKKFKDPFHDPIKWALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXXXXXXXXXXXXXXX------XXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQ-----LSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIR-FCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            F P R    GT G+ I L+ N F + +    ++ YDIE+ K E       R+  + +      R    Q   D+   P +DG R ++    LP       L      E ++    ++LQ V+    +  +   + +  G  N +P+++++ LD+I R  L       +G      P            +  G+  ++RP M N+  N+D +    ++A  + +++ +V  + N N Q K   +S+       I  +KV+ +H          R + V  V ++ A ++     +EN   +  +V +YF   + + LKYP L  ++     K    P E+  I+  Q    K+  N  + + +    SA    E    L KS     G   + YL+ + I +   M  V GR+L  P   +G             +K    P +  +++  K+F       + WA+  F  +++   E+ L +   +L K +    + I     FC  +              +  + + +           Y  VK +GD  LG+ TQC+  +N+   K   Q + NL LK+NAKLGG+N  +   +   +    ++F G DVTHP   DG   S+AAVVGS++   + Y A ++ Q     LS++   + E++ ++ +MV+ L+  FY   + K P  +I+YR GVSEGQ + V   E+ AI+ AC   +++Y+P ITYI+VQKRH+ R FC    E  VG+  NV  G  +D+ ITH  EFDFYLCSH GIQGTS+P HY+V+ D+N + +++LQ  TY LC+   RCTRS+S PAP +YA L AFR R    D+D+ S    + S
Sbjct:   15 FQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDIEI-KPEKRPRRVNREVVDTM-----VRHFKMQIFGDR--QPGYDGKRNMYTAHPLPIGRDRVDLEVTLPGEGKDQTFKVSLQWVS----VVSLQMLLEALSGHLNEVPEDSVQALDVITRH-LPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPVIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQ-----MKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGG--PDPYLKEFGIVVHNDMTEVTGRVLPAPMLQYGGRVSTDTGRDCGRNKTVATPNQGVWDMRGKQFYAGIEIKV-WAVACFAPQKQC-REDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMSYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVV--KTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQDLSQEQLYSQEVIQDLTNMVRELLIQFYKSTRFK-PTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCSDKAE-RVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVS 846          
BLAST of Protein argonaute-3 vs. Ensembl Cavefish
Match: ago4 (protein argonaute-4 [Source:NCBI gene;Acc:103043810])

HSP 1 Score: 311.612 bits (797), Expect = 3.842e-91
Identity = 226/763 (29.62%), Postives = 368/763 (48.23%), Query Frame = 3
Query:  708 PIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEIIENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQ----KVMGNDPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXXXXXXXXXXXXXXX------XXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQ-----LSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIR-FCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            P +DG R ++    LP       L      E ++    ++LQ V+    +  +   + +  G  N +P+++++ LD+I R  L       +G      P            +  G+  ++RP M N+  N+D +    ++A  + +++ +V  + N N Q K   +S+       I  +KV+ +H          R + V   +     N   + +  +V +YF   + + LKYP L  ++     K    P E+  I+  Q    K+  N  + + +    SA    E    L KS     G   + YL+ + I +   M  V GR+L  P   +G + + +      + + + K  F+  I+   WA+  F  +++   E+ L +   +L K +    + I     FC  +              +  + + +           Y  VK +GD  LG+ TQC+  +N+   K   Q + NL LK+NAKLGG+N  +   +   +    ++F G DVTHP   DG   S+AAVVGS++   + Y A ++ Q     LS++   + E++ ++ +MV+ L+  FY   + K P  +I+YR GVSEGQ + V   E+ AI+ AC   +++Y+P ITYI+VQKRH+ R FC    E  VG+  NV  G  +D+ ITH  EFDFYLCSH GIQGTS+P HY+V+ D+N + +++LQ  TY LC+   RCTRS+S PAP +YA L AFR R    D+D+ S    + S
Sbjct:   15 PGYDGKRNMYTAHPLPIGRDRVDLEVTLPGEGKDQTFKVSLQWVS----VVSLQMLLEALSGHLNEVPEDSVQALDVITRH-LPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPVIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQ-----MKRKYRVSSKFFPLLENG--QAMECTVAQYFKQKYSLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGG--PDPYLKEFGIVVHNDMTEVTGRVLPAPMLQYGGRNKTVATPNQGVWDMRGKQ-FYAGIEIKVWAVACFAPQKQC-REDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMSYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVV--KTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQDLSQEQLYSQEVIQDLTNMVRELLIQFYKSTRFK-PTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCSDKAE-RVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVS 757          
BLAST of Protein argonaute-3 vs. Ensembl Cavefish
Match: ago1 (argonaute RISC component 1 [Source:ZFIN;Acc:ZDB-GENE-110606-3])

HSP 1 Score: 298.901 bits (764), Expect = 2.169e-87
Identity = 195/640 (30.47%), Postives = 318/640 (49.69%), Query Frame = 3
Query: 1050 GYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXXXXXXXXXXXXXXX------XXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVN-WEIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNIS 2885
            G+  ++RP M  +  N+D +    ++A  + +++ +V  + N + Q K   +S+       I  +KV+ +H          R + V  V ++ A ++         + +  +V +YF   + + LKYP L  ++     K    P E+  I+  Q+ +     N    +IK +A    +R + + + ++    F  + Y+Q + I +   M  V GR+L  P   +G +   +      + + + K  F++ I+   WA+  F  +++   E  L     +L K +    + I     FC  +                   + +           Y  VK +GD  LG+ TQC+  +N+   K   Q + NL LK+N KLGG+N   + +   A  +   +  G DVTHP   DG   S+ AVVGS++   + Y A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  +IFYRDGV EGQ   + ++E+ AI+ AC K +K+Y+P ITYI+VQKRH+ R       + +G+  N+  G  +D  ITH  EFDFYLCSH GIQGTS+P HYYV+ D+NR+ +++LQ  TY LC+   RCTRS+S PAP +YA L AFR R    D+++ S
Sbjct:   45 GFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKTLTDSQRVRFTKEIKGLKVEVTHCGQ-----MKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISR-LMKNANFNLDPYIQEFGIKVRDDMAEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQ-FYNGIEIKVWAIACFAPQKQ-CREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVV--KTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQ-----EIIEDLSYMVRELLIQFYKSTRFK-PTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKSERIGKSGNIPAGTTVDTSITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDS 668          
BLAST of Protein argonaute-3 vs. Ensembl Sea Lamprey
Match: ago2 (argonaute RISC catalytic component 2 [Source:ZFIN;Acc:ZDB-GENE-110606-6])

HSP 1 Score: 222.631 bits (566), Expect = 9.201e-66
Identity = 112/247 (45.34%), Postives = 157/247 (63.56%), Query Frame = 3
Query: 2169 MVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVV-IDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            +  G +VTHP   DG   S+AAVVGS++   + Y A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  +IFYRDGVSEGQF+ V  HE+ AI+ AC K +K+Y+P ITY+ VQKRH+ R       + +G+  N+  G   +D  ITH  EFDFYLCSH GIQGTS+P HY+V+ D+NR+++++LQ  TY LC+   RCTRS+S PAP +YA L AFR R    D+++ S    + S
Sbjct:   11 IFLGAEVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQHHRQ-----EIIQDLGTMVRELLIQFYKSTRFK-PTRIIFYRDGVSEGQFQQVLFHELLAIRDACIKLEKDYQPGITYVAVQKRHHTRLFCADRNERIGKSGNIPAGTTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSADELQVLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSAEGSHVS 251          
BLAST of Protein argonaute-3 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009610.1 (pep scaffold:Pmarinus_7.0:GL477777:44890:66775:1 gene:ENSPMAG00000008651.1 transcript:ENSPMAT00000009610.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 210.69 bits (535), Expect = 3.283e-57
Identity = 194/742 (26.15%), Postives = 330/742 (44.47%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAEL-------VLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGLPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSG-KGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHD-PIK-WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXX------XXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVN-WEIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIR-FCPITLEDSVGRCKN 2600
            P RP   GT G+ I L  N F + +    +  YDI++   +        V+E+ V+ F +KI     F D            P+FDG + ++    LP      +L      E +     + ++ + +          +H    GR   IP E+I+ LD+++R  L       +G      P    Q       +  G+  ++RP M  +  N+D +    ++A  + +++ +V  L     Q +   +S+       I  +KV+ +H          R + V  V ++ A  +         + +  +V +YF   + ++LKY  L  ++     K    P E+  I+  Q+ +     N    +IK +A    +R + + + ++    F ++ Y++ + I +   M +V+GR+L+ P   +G +   +      + + + K  F    IK WA+  F  +R+   E  L     +L K +    + I     FC  +                      ++V++      Y  VK +GD  LG+ TQC+  +N+   K   Q + NL LK+N KLGGVN   + +I  A  +   +  G DVTHP   DG   S+AAVVGS++   + Y A ++ Q  +      EI+ ++  MV+ L+  FY   + K P  +IFYRDGVSEGQF+ V  HE+ AI+ AC + +K Y+P IT+I+VQKRH+ R FC    E     CK+
Sbjct:   27 PLRPG-FGTVGKPIQLRANYFEVDIPKMDLLHYDIDIKPEKCPRRVNREVVEHMVQHFKHKI-----FGD----------RKPVFDGRKNLYTVTPLPIGRDRVELEVTLPGEGKERVFKVTIKWMANVSLQ-----LLHEALSGRLAQIPYESIQGLDVVMRH-LPSMRYTPVGRSFFTPPEGYSQPLGGGREVWFGFHQSVRPCMWKMMLNIDVSATAFYKAQPVIEFMCEVLDLRTVEEQPRPLSDSQRVKFTKEIKGLKVEITHCGQ-----MKRKYRVCNVTRRPAAQQTFPLQLESGQTVECTVAQYFRDKYKLHLKYAHLPCLQVGQEEKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISR-LMKSASFNSDPYVREFGIMVKDDMADVQGRVLNPPMLQYGGRTRAVATPNQGVWDMRGKQFFTGIEIKVWAIACFAPQRQC-REEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGAENVEPMFRHLKNAYMGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVL--KTSPQTLSNLCLKINVKLGGVNNILVPHIRPAVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQQHRQ-----EIIHDLSSMVRELLIQFYKSTRFK-PTRIIFYRDGVSEGQFQQVLYHELLAIREACIRLEKEYRPGITFIVVQKRHHTRLFCADRNERVSDACKS 731          
BLAST of Protein argonaute-3 vs. Ensembl Sea Lamprey
Match: piwil2 (piwi-like RNA-mediated gene silencing 2 [Source:NCBI gene;Acc:572134])

HSP 1 Score: 149.828 bits (377), Expect = 3.565e-37
Identity = 103/304 (33.88%), Postives = 159/304 (52.30%), Query Frame = 3
Query: 1974 EYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSK-SVAAVVGSINKTAT-IYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAH-LAAFRGRILKDDED 2876
             Y  VK +  +EL + +Q +    + +QK L      ++L++N KLGG  W    I   P+K L MV GVDV H   N  L K SV   V S+N++ +  YS V+++  S++  D + + +         IK +Y+LN   LP+ +I YRDGVS+ Q  IV + E+  +      F  +Y+PK+  ++VQKR +     I L +S G  +N   G V+D   T    FDF+L S    QGT  P HY VI +  + +++ +Q  TY + ++ +  + ++  PAP  YAH LA+  G  L  D D
Sbjct:  481 RYSTVKQVCCVELAVPSQVVQTRTISQQKRLRSVAQKVVLQMNCKLGGELW----ILDIPLKNL-MVVGVDVFH---NVVLGKQSVVGFVASMNRSVSRWYSRVVRQSTSQELVDGLNLCMTSA------IKKYYELNH-YLPEKIIMYRDGVSDNQLAIVRDFEVMQLTNGFRTFDLSYQPKMLVVVVQKRISTN---IFLNNS-GAMENAPSGSVLDTVATSQEWFDFFLVSQHVRQGTVSPTHYVVIFNSLKLSADHVQRLTYKMTHLYYNWSGTVRVPAPCQYAHKLASLVGENLHSDPD 765          
BLAST of Protein argonaute-3 vs. Ensembl Sea Lamprey
Match: piwil1 (piwi-like RNA-mediated gene silencing 1 [Source:NCBI gene;Acc:368200])

HSP 1 Score: 100.138 bits (248), Expect = 1.271e-21
Identity = 82/292 (28.08%), Postives = 135/292 (46.23%), Query Frame = 3
Query: 1974 EYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTAT-IYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAF 2846
            +Y  +K    +E    +QC+    L + + +      + L++N KLGG  W ++     PIK L MV GVD  H S   G  +SV   V S+N+T T  YS  I +  +++  D +++ ++ ++ +   +++    N   +   +I++   ++                Y C       +PK+T ++V+KR   RF     E      +N  PG VID + T    +DFYL S    QGT  P HY V+ D +    + +Q   Y +C++ +     I  PAP HYAH  AF
Sbjct:  586 KYDSIKQFLCVERPFPSQCVLARTLSKPQMIRSVATKIALQMNCKLGGELWSVE----IPIKQL-MVVGVDSYHDSITRG--QSVCGFVASLNQTLTRWYSRCIFQHAAQEMIDGLKVCMQCKNFIS--LQIASHSNDVMINVVIIYFLSFLNS---------------YCC-----GSRPKVTVVVVKKRGIARF----FEFGRSGLQNPSPGTVIDTEATRHEWYDFYLVSQSVRQGTVTPTHYNVVYDTSNMKPDHMQRLAYKMCHLYYNWPGVIRVPAPCHYAHKLAF 844          
BLAST of Protein argonaute-3 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009316.1 (pep scaffold:Pmarinus_7.0:GL484064:2408:13690:1 gene:ENSPMAG00000008422.1 transcript:ENSPMAT00000009316.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 65.0846 bits (157), Expect = 2.836e-11
Identity = 65/331 (19.64%), Postives = 135/331 (40.79%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGLPIFDGGRIIFNNQLLPYT-ETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSG-KGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK 1427
            P RP  G T G+ I L+ N F + +   ++  YD+++   +     N       + R ++      QF +D+   P+FDG + ++    LP   + + +L      E ++    + L+ V            +H    GR   +PQ+ ++ +D+++R +        +G     +P            +  G+  ++RP M  +  N+D +    ++A  + +++ +V  + N + Q K   +S+       I  +KV+ +H          R + V  V ++ A ++         + +  +V  YF   H + LKY  L  ++     K
Sbjct:   21 PRRPGLG-TVGKHIKLLANYFEVDIPKIEVHHYDLDIKPEKCPRRVNREVVDYLVNRSNV------QFFRDR--KPVFDGRKNLYTASALPVGRDKVVELEVTLPGEGKDRVFKVALKWVGYVSWHA-----LHDALAGRIMQVPQDPVQAVDVVLRHLPSMRRYTPVGRSFFSSPEGYSHPLGGGREVWFGFHQSVRPSMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVKFTKEIKGLKVEITHCG-----QMKRKYKVCNVTRRPASHQTFPLQLDTGQTVECTVAHYFRDRHKLQLKYAHLPCLQVGQEQK 332          
BLAST of Protein argonaute-3 vs. Ensembl Nematostella
Match: EDO46431 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQ46])

HSP 1 Score: 337.806 bits (865), Expect = 2.038e-100
Identity = 256/848 (30.19%), Postives = 421/848 (49.65%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGL-PIFDGGRIIFNNQLLPYTETINKLNFIYNYEN-QNINCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQ--LDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINS--------RIYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEIIENLGKS----VNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGNDPNI-VREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKE--EKLPKSKFNIMNKKFKDPFH--DPIKWALFAFGERREYGNENELYAIEIELPKAAIKVNVKI-LKDNKFCYVSNXXXXXXXXXXXXXXXXXXXXXXXXXE-----YKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAP--IKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGR---ILKDDE---DNISLSSENESIG 2912
            P+RP+ G T GR I L  N F + +    I  YD+ +   +     N          +D+   +V  + K   G+ P+FDG + ++    LP  +T  +   +   +N Q+    + L+ V+      L        +G  N IP ETI+ LD+++R  L       +G +  F+P       L+R    +  G+  +IRP    +  N+D +    ++  ++ +++ +V  +   +++    +N          I  +KV+ +H        Y  I   K   Q       +E+ G++    V  YF   H   L+YP L  ++     +    P E+  I+P Q+ +    +    ++I+ +A    +R   ++  +V    F  + Y++ ++I+I   M+ ++GR+L  P  ++G K   EK+  S   + + + +  FH  +   WA+  F  +++   E+ L     +L K +++  + I      F Y  N D  + + +  ++    + ++   +      Y  VK +GD  LG++TQCI  +N+   K   Q + NL LK+NAKLGGVN  I   E  P   +   +  G DVTHP+  D    SVAAVVGS++   + Y A ++ Q  +      EI+ E+  MV+ L+  FY   + K P  ++FYRDGVSEGQF  V  HE+ AI+ AC K +  Y+P I++I+VQKRH+ R      +D  G+  NV PG  +D  ITH  EFDFYLCSH GIQGTS+P HY+V+ D+N +++++LQ  TY LC+   RCTR++S PAP +YAHL AFR R   + +D E   DN S++S  +  G
Sbjct:   17 PKRPNHGST-GRTIALRANFFPVQLPRGNIHHYDLSICPDKCPRRVN----------RDVVEVMVLNYHKVFGGMKPVFDGRKNLYTRDPLPIGKTPTEFEVVLPTDNSQDKKFKVTLKWVSQVSLYALEKAL----EGTCNQIPFETIQALDVVLRH-LPSMKYTPVG-RSFFSPPDGYDFPLERG-REVWFGFHQSIRPSQWKMLLNIDVSATAFYKCQSVVEFMCEVLRIRKDDLEKHAPLNDADRLRFTREIKGLKVEITHC-GPMKRKYRVINVTKQPAQALQFPLTLES-GQTAQITVARYFQDKHKQRLRYPHLPCLQVGQEQRHTYLPLEVCNIVPGQRCVKKLTDTQTSKMIRATARSAPDREREIR-GLVKKANFDEDAYVKDFSISIGKNMVELQGRVLPPPKLVYGGKVRIEKIIHSFSGVWDMRGRQLFHGIEIRTWAIACF-VKQQMCTEDSLRRFSNQLMKISVEQGMPISCPPVFFRYARNPDEVERMFRRLKEAHPDLQMILVVLPGKTPVYAEVKRVGDTMLGVITQCIQGKNVT--KPSPQTLSNLCLKINAKLGGVN-NILAPEIRPPVFREPVIFLGADVTHPAAGDDKRPSVAAVVGSMDAHPSRYYASVRVQTHRQ-----EIIAELAAMVRELLVQFYRSTRHK-PQRIVFYRDGVSEGQFRQVLVHELKAIREACIKLEVGYQPGISFIVVQKRHHTRLFCQRDQDKCGKSGNVPPGTTVDRGITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNNFSADELQALTYQLCHTYVRCTRAVSIPAPAYYAHLVAFRARYHMMDRDKEAGNDNSSVASGEDCRG 833          
BLAST of Protein argonaute-3 vs. Ensembl Nematostella
Match: EDO38583 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCI6])

HSP 1 Score: 157.147 bits (396), Expect = 1.879e-39
Identity = 94/246 (38.21%), Postives = 135/246 (54.88%), Query Frame = 3
Query: 1977 YKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIK-----GLF----MVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDM-VQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQ 2684
            Y  VK +GD  LGI TQC+  +     K   Q   N+ +K+N KLGG N  I +   A I      G+F    ++FG DVTHP+  D    S+AAVV S+N+ A+ Y A ++ Q     K A EI+V++ DM V   I+++  L      D    +R      +F+++   E+ A+Q AC   +K+Y+P IT+++VQKRH+ R       D+ G+ +NV  G  +D  I H  EFDFYLCSH GIQ
Sbjct:  510 YNEVKRVGDTVLGIPTQCVQMKQFTMAK--PQVCSNIAMKINGKLGGTNHVIADSLKATITDDKGNGIFNSPVIIFGADVTHPAPGDNGIPSIAAVVASLNRNASRYCARVRPQTHMKCKQAQEIIVDLADMDVHIYIRLWRKLESH---DTQTMFR------RFQVLL-EEVRAVQQACAMLEKDYQPLITFVVVQKRHHARLFAEEGRDARGKSRNVPAGTTVDTVICHPFEFDFYLCSHAGIQ 743          

HSP 2 Score: 91.2781 bits (225), Expect = 1.197e-18
Identity = 93/414 (22.46%), Postives = 180/414 (43.48%), Query Frame = 3
Query:  480 LPERPDKGGTKGRQIPLIVNCFNMTVKSTK-IFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGLPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLD-RDPYTILSGYFSTIRP-HMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNI---------QNKKHINSRIYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNE--IIENLGKSVNEYFLSTHGINLKYPEL-LLIETTNYNKFPAELLTILPNQKVMGNDPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFG 1676
            LP+RP  G  +GR+I L  N F +T+ +   ++ YD+E+   E    +  RD  +   +   F+ V      + C  P FDG R ++  + LP       L       +        L++  + +      D   +G+ R   +PQ+ I+ +DI++R+M        +G +  F P      D      + +G++ ++RP     +  N+D +     +++ + D++ ++   +   +         ++KK + + I  ++V+ +H K        R FTV G+     +N   + +N   +V  YF + + I+L+YP L  L+     N  P E+  ++P Q+    D      +I+ +A P  ER  ++ + V       +      Y + I  QM+ ++GR+L  P    G
Sbjct:    6 LPKRPGYG-REGRKIKLRANFFQVTLPNVDFLYHYDLEI-SPEKAPVSVCRDVVDAAIKHGEFKGVF-----NGCK-PAFDGRRNLYCREPLPLKSEEASLKVTLPGTDGGKERKFTLKIKEAGLVSIKELDQFLNGEFRGK-VPQDAIQGMDIVLRQMP-SMKFTAVG-RCFFPPPNGHCHDLGGGCELWTGFYQSVRPSQWKTMLLNIDVSSKGFQKSMPVIDFMLEILRQDRNRVLDTRWVMDERDKKKLTTEIKGLRVETTHIK--------RKFTVMGLSHPAFNNRFRLEDNTETTVEAYFRNKYNISLRYPHLPCLLVGQKKNSVPMEVCNMIPTQRKRLTDEQTA-AMIRKTAKPANERQRDINQWVDELATASDQYLKNEYGMRINKQMVAIEGRVLPAPELTLG 399          
BLAST of Protein argonaute-3 vs. Ensembl Nematostella
Match: EDO34027 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQI3])

HSP 1 Score: 144.05 bits (362), Expect = 3.445e-35
Identity = 88/292 (30.14%), Postives = 153/292 (52.40%), Query Frame = 3
Query: 1974 EYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFR 2849
             Y  VK +  +E  + +Q I  + +     L   +  + L++N KLGG  W +      P + L MV G+DV H     G  +SV  +V S+N++ T + + +  Q         E++  ++ ++ + I+ ++D+N + LP+ +I YRDGV +GQ ++V  +E+   +     F ++YKPK+  ++VQKR N R    + E  +G   N  PG V+D+ +T     DF+L S    QGT  P HY V+ D + W  + +Q+ +Y L ++ +    ++  PAP  YAH  A++
Sbjct:  454 RYAAVKKLCCVESPVPSQVIISKTISNPTKLRSVVQKIALQINVKLGGELWALD----IPSRSL-MVCGIDVYHDKARGG--RSVGGLVCSMNRSLTRWYSDVCFQ-----SPGQELIDGLKMLLIKGIRKWHDVNNA-LPERIIVYRDGVGDGQLKVVAGYEVQQFEECFASFGESYKPKLAVLVVQKRINTRI--FSAEPRLG---NPGPGSVLDHTVTRKDWNDFFLVSQHVRQGTVTPTHYVVVYDTSNWKPDIMQKLSYKLTHLYYNWPGTVRVPAPCQYAHKLAYQ 727          
BLAST of Protein argonaute-3 vs. Ensembl Nematostella
Match: EDO49931 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFC2])

HSP 1 Score: 142.124 bits (357), Expect = 2.450e-34
Identity = 88/296 (29.73%), Postives = 142/296 (47.97%), Query Frame = 3
Query: 1974 EYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTAT----IYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGR-CKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAF 2846
             Y  +K +  +E G+ +Q +    L +++ L      + +++N KLGG  W +      P+K L M+ G+D  H S+  G  +SV   V S+NK  T     YS    +   ++  D +++ +         ++ +  +N   LPD +I +RDGV +GQ   V  HE+  I    ++F+  Y PK  + +V+KR   R       D   R   N  PG V+D ++T S  FDF++ S    QGT  P HY VI D      +  Q+ +Y L ++ +    ++  PAP  YAH  AF
Sbjct:  569 RYDSIKKLCCLEKGVPSQVVVSRTLSKKQMLMSVCTKIGIQLNCKLGGEAWAVD----IPLKNL-MIVGIDTYHDSSQKG--RSVGGFVASLNKPCTRQREYYSRCTFQHSGQELVDGLKVCMTAA------LRQWQQIN-GVLPDKIIVFRDGVGDGQLRAVIEHEVPQILSTFKQFQDGYDPKFGFTVVKKRIQTRLFMREARDPDMRNLMNPPPGTVVDKEVTRSEWFDFFIVSQSVRQGTVSPTHYNVIHDTTGLRPDHWQQLSYKLTHLYYNWPGTVRVPAPCQYAHKLAF 850          
BLAST of Protein argonaute-3 vs. Ensembl Nematostella
Match: EDO28124 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T7C9])

HSP 1 Score: 59.6918 bits (143), Expect = 3.926e-10
Identity = 26/68 (38.24%), Postives = 45/68 (66.18%), Query Frame = 3
Query: 2352 RLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIR 2555
            + I+ ++D+N + LP+ +I YRDGV +GQ ++V  +E+   +     F ++YKPK+  ++VQKR N R
Sbjct:  109 KAIRKWHDVNNA-LPERIIVYRDGVGDGQLKVVAGYEVQQFEECFASFGESYKPKLAVLVVQKRINTR 175          
BLAST of Protein argonaute-3 vs. Ensembl Medaka
Match: AGO2 (protein argonaute-2 [Source:NCBI gene;Acc:101156100])

HSP 1 Score: 346.28 bits (887), Expect = 4.414e-103
Identity = 250/847 (29.52%), Postives = 412/847 (48.64%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCG--LPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSG-KGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPR--TSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXX------XXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-------GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            P RPD  GT GR I L  N F M +   +++ YDI++   +     N          ++I   +V  F     G   P++DG + ++    LP      +L      E ++ +  + ++ V+           +H    GR  ++P ETI+ LD+++R  L       +G +  F P    S  L      +  G+  ++RP +  +  N+D +    ++A  + +++ +V    +   Q K   +S+       I  +KV+ +H          R + V  V ++ A ++         + +  +V +YF   + + L+YP L  ++     K    P E+  I+  Q+ +     N    +I+ +A    +R D + K ++    F  + Y++ + + +  +M  V GR+L  P+ L+G + + +      + + + K  FH  I+   WA+  F  +R+   E  L A   +L K +    + I     FC  +                 +    ++V++      Y  VK +GD  LG+ TQC+  +N+  QK   Q + NL LK+N KLGGVN    NI     +G  MVF       G DVTHP   DG   S+AAVVGS++   + Y A ++ Q  +      +I+ ++ +MV+ L+  FY   + K P  +I+YRDG+SEGQF  V  HE+ AI+ AC K +K+Y+P IT+++VQKRH+ R   +   + VG+  N+  G  +D  ITH  EFDFYLCSH GIQGTS+P HY+V+ D+N ++S++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:   21 PSRPD-FGTMGRTIKLQANFFEMEIPKLEVYHYDIDIKPEKCPRRVN----------REIVEHMVQHFKTQIFGDRKPVYDGRKNLYTAMALPIGREKVELEVTIPGEGKDRSFKVAIKWVSCVSLQA-----LHEALSGRLPSVPFETIQALDVVMRH-LPSMRYTPVG-RSFFTPSEACSNPLGGG-REVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFMCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQ-----MKRKYRVCNVTRRPASHQTFPLQQENGQTIECTVAQYFKDKYKLILRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQDEISK-LMRSANFNTDPYVREFGVMVRDEMTEVNGRVLQAPSILYGGRNKAIATPIQGVWDMRNKQ-FHTGIEIKVWAIACFAPQRQC-TELLLKAFTDQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKYTYQGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQVKNV--QKTTPQTLSNLCLKINVKLGGVN----NILLP--QGRPMVFQQPVIFLGSDVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQQHRQ-----DIIQDLANMVRELLIQFYKSTRFK-PTRIIYYRDGISEGQFNQVLQHELLAIREACIKLEKDYQPGITFVVVQKRHHTRLFCMDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNHFSSDELQVLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTS 826          
BLAST of Protein argonaute-3 vs. Ensembl Medaka
Match: ENSORLT00000011404.2 (protein argonaute-3 [Source:NCBI gene;Acc:101165653])

HSP 1 Score: 324.324 bits (830), Expect = 3.679e-95
Identity = 239/852 (28.05%), Postives = 401/852 (47.07%), Query Frame = 3
Query:  480 LPERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELL--KAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGLPIFDGGRIIFNNQLLPYTETINKLNFIYNYEN-QNINCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQE--------TIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXXXXXXXXXXXXXXX------XXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVN-----WEIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            LP+RP  G T G+ I L+ NCF + +    +++Y++++   K    +   V D   +  +  IF D           LP++DG + ++    LP       L+     E  ++    ++L+ V+      L D      KGRS   P +         +  +D+++R  L       +G     +P+ +         +  G+  ++RP M  +  N+D +    ++A ++  ++ +V  ++N + Q +   +S        I  +KV+ +H    +     R + V  V ++ A  +         + + ++V +YF   + + LKYP L  ++     K    P E+  I+  Q+ +     N    +IK +A    +R + + + +V    +  + ++Q +   +  +M  V GR+L  P   +G +   +      + + + K  FH  ++   WA+  F  +R+   E  L +   +L K +    + I     FC  +                   + +           Y  VK +GD  LG+ TQC+  +N+   K   Q + NL LK+N KLGG+N      +  ++   PI    +  G DVTHP   DG   S+AAVVGS++   + Y A ++ Q     K   EI+ ++  MV+ L+  FY   + K P  +IFYRDGVSEGQF  V  +E+ AI+ AC   +K Y+P IT+I+VQKRH+ R       + VGR  N+  G  +D DITH  EFDFYLCSH GIQGTS+P HY+++ D+N + +++ Q  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:   17 LPQRPGYG-TIGKAIKLLANCFQVDIPKLDVYLYEVDIKPDKCPRRVNREVVDSMVQHFKVTIFGD----------RLPVYDGKKNLYTASPLPVASGGVDLDVTLPGEGGKDRPFKVSLKFVSLVSWHLLHDIL----KGRSTPEPVDLDKPLSTNPVHAVDVVLRH-LPSMKYTPVGRSFFSSPKDNDYTLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQSVIQFMCEVLDIHNIDEQPRPLADSHRVKFTKEIKGLKVEVTHCGTMR-----RKYRVCNVTRRPASLQTFPLQLENGQTVERTVAQYFREKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVVGQRCIKKLTDNQTSIMIKATARSAPDRQEEISR-LVRNANYGADPFVQEFQFRLCDEMAQVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQ-FHTGVEIKVWAIACFATQRQC-REEILKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYTGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQMKNVV--KTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPI----IFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRIQ-----KPRQEIIQDLASMVRELLIQFYKSTRYK-PTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKEYQPGITFIVVQKRHHTRLFCADRNERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHILWDDNCFTADEFQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHIS 832          
BLAST of Protein argonaute-3 vs. Ensembl Medaka
Match: AGO2 (protein argonaute-2 [Source:NCBI gene;Acc:101156100])

HSP 1 Score: 325.479 bits (833), Expect = 7.186e-95
Identity = 248/875 (28.34%), Postives = 410/875 (46.86%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCG--LPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSG-KGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPR--TSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXX------XXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-------GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGI-------------------------------------QGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDN 2879
            P RPD  GT GR I L  N F M +   +++ YDI++   +     N          ++I   +V  F     G   P++DG + ++    LP      +L      E ++ +  + ++ V+           +H    GR  ++P ETI+ LD+++R  L       +G +  F P    S  L      +  G+  ++RP +  +  N+D +    ++A  + +++ +V    +   Q K   +S+       I  +KV+ +H    +     R + V  V ++ A ++         + +  +V +YF   + + L+YP L  ++     K    P E+  I+  Q+ +     N    +I+ +A    +R D + K ++    F  + Y++ + + +  +M  V GR+L  P+ L+G + + +      + + + K  FH  I+   WA+  F  +R+   E  L A   +L K +    + I     FC  +                 +    ++V++      Y  VK +GD  LG+ TQC+  +N+  QK   Q + NL LK+N KLGGVN    NI     +G  MVF       G DVTHP   DG   S+AAVVGS++   + Y A ++ Q  +      +I+ ++ +MV+ L+  FY   + K P  +I+YRDG+SEGQF  V  HE+ AI+ AC K +K+Y+P IT+++VQKRH+ R   +   + VG+  N+  G  +D  ITH  EFDFYLCSH GI                                     QGTS+P HY+V+ D+N ++S++LQ  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++
Sbjct:   77 PSRPD-FGTMGRTIKLQANFFEMEIPKLEVYHYDIDIKPEKCPRRVN----------REIVEHMVQHFKTQIFGDRKPVYDGRKNLYTAMALPIGREKVELEVTIPGEGKDRSFKVAIKWVSCVSLQA-----LHEALSGRLPSVPFETIQALDVVMRH-LPSMRYTPVG-RSFFTPSEACSNPLGGG-REVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFMCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMK-----RKYRVCNVTRRPASHQTFPLQQENGQTIECTVAQYFKDKYKLILRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQDEISK-LMRSANFNTDPYVREFGVMVRDEMTEVNGRVLQAPSILYGGRNKAIATPIQGVWDMRNKQ-FHTGIEIKVWAIACFAPQRQ-CTELLLKAFTDQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKYTYQGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQVKNV--QKTTPQTLSNLCLKINVKLGGVN----NILLP--QGRPMVFQQPVIFLGSDVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQQHRQ-----DIIQDLANMVRELLIQFYKSTRFK-PTRIIYYRDGISEGQFNQVLQHELLAIREACIKLEKDYQPGITFVVVQKRHHTRLFCMDRNERVGKSGNIPAGTTVDTKITHPSEFDFYLCSHAGIQVRDQRKMAELNESPSCALSVSAWFDGGKAFINALYLQGTSRPSHYHVLWDDNHFSSDELQVLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEH 910          
BLAST of Protein argonaute-3 vs. Ensembl Medaka
Match: ago4 (protein argonaute-4 [Source:NCBI gene;Acc:101167465])

HSP 1 Score: 321.627 bits (823), Expect = 1.900e-93
Identity = 248/858 (28.90%), Postives = 402/858 (46.85%), Query Frame = 3
Query:  477 FLPERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGLPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEI----IEN---LGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQ----KVMGNDPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFG-------------DKEEKLP-KSKFNIMNKKFKDPFHDPIKWALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXXXXXXXXXXXXXX------XXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKD-----AVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIR-FCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            F P R    GT G+ I L+ N F + +    ++ YDI++ K E       R+  + +      R    Q   D+   P +DG R ++    LP       L      E ++    ++LQ V+    +  +   + +  G  N +P+++++ LD+I R  L       +G      P            +  G+  ++RP M N+  N+D +    ++A  + +++ +V  + N N Q K   +S+       I  +KV+ +H          R + V  V ++ A ++     +EN   +  +V +YF   + + LKYP L  ++     K    P E+  I+  Q    K+  N  + + +    SA    E    L KS     G   + YL+ + I +   M  V GR+L  P   +G             +K    P +  +++  K+F       + WA+  F  +++   E+ L +   +L K +    + I     FC  +              +  + +             Y  VK +GD  LG+ TQC+  +N+   K   Q + NL LK+NAKLGG+N  +   +   +    ++F G DVTHP   DG   S+AAVVGS++   + Y A ++ Q S+         + E++ ++ +MV+ L+  FY   + K P  +I+YR GVSEGQ + V   E+ AI+ AC   +++Y+P ITYI+VQKRH+ R FC    E  VG+  NV  G  +D+ ITH  EFDFYLCSH GIQGTS+P HY+V+ D+N + +++LQ  TY LC+   RCTRS+S PAP +YA L AFR R    D+D+ S    + S
Sbjct:   88 FQPPRRPGLGTVGKPIRLLANHFQVQIPKIDVYHYDIDI-KPEKRPRRVNREVVDTM-----VRHFKMQIFGDR--QPGYDGKRNMYTAHPLPIGRDRVDLEVTLPGEGKDQTFKVSLQWVS----VVSLQMLLEALSGHLNEVPEDSVQALDVITRH-LPSMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWNMMLNIDVSATAFYRAQPVIEFMCEVLDIQNINEQTKPLTDSQRVKFTKEIRGLKVEVTHCGQ-----MKRKYRVCNVTRRPASHQTFPLQLENGQAMECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVKSNSMVGG--PDPYLKEFGIVVHNDMTEVTGRVLPAPMLQYGGRVSTDTGRDCGRNKTVATPNQGVWDMRGKQFYAGIEIKV-WAVACFAPQKQC-REDLLKSFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFKHLKMSYVGLQLIVVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVV--KTSPQTLSNLCLKINAKLGGINNVLVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQDMSQEQLFSQEVIQDLTNMVRELLIQFYKSTRFK-PTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHHTRLFCSDKAE-RVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKDHDSAEGSHVS 919          
BLAST of Protein argonaute-3 vs. Ensembl Medaka
Match: ago3a (protein argonaute-3 [Source:NCBI gene;Acc:101157320])

HSP 1 Score: 310.071 bits (793), Expect = 1.509e-90
Identity = 218/770 (28.31%), Postives = 362/770 (47.01%), Query Frame = 3
Query:  708 PIFDGGRIIFNNQLLPYTETINKLNFIYNYEN-QNINCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQE--------TIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSR-------IYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGN-DPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIK---WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXXXXXXXXXXXXXX------XXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVF-GVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENES 2906
            P++DG R ++    LP   T   L+     E  ++    ++++ V+      L +       GRS   P E         +  +D+++R  L       +G      P            +  G+  ++RP M  +  N+D +    ++A  +  ++ +V  ++N + Q +   +S        I  +KV+ +H    +     R + V  V ++ A ++         + + ++V +YF   + + LKYP L  ++     K    P E+  I+  Q+ +     N    +IK +A    +R + + + +V    +  + ++Q +   +  +M +V GR+L  P   +G +   +      + + + K  FH  ++   WA+  F  +R+   E  L     +L K +    + I     FC  +                   +             Y  VK +GD  LG+ TQC+  +N+   K   Q + NL LK+N KLGG+N  +   +   +    ++F G DVTHP   DG   S+AAVVGS++   + Y A ++ Q  +      E++ ++  MV+ L+  FY   + K P  +IFYRDGVSEGQF  V  +E+ AI+ AC   +K Y+P ITYI+VQKRH+ R       + VGR  N+  G  +D DITH  EFDFYLCSH GIQGTS+P HY+V+ D+N + +++ Q  TY LC+   RCTRS+S PAP +YAHL AFR R    D+++ S    + S
Sbjct:   15 PVYDGKRSLYTANPLPVAPTGVDLDVTLPGEGGKDRPFKVSIKFVSLVSWHMLHEVLT----GRSMPEPLELDKPISTNPVHAVDVVLRH-LPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMR-----RKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISR-LVRSANYEADPFVQEFQFRVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQ-FHTGVEIKMWAIACFATQRQC-REEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVV--KTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQ-----EVIQDLASMVRELLIQFYKSTRYK-PTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKEYQPGITYIVVQKRHHTRLFCADRNERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADEFQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVS 763          
BLAST of Protein argonaute-3 vs. Planmine SMEST
Match: SMESG000051000.1 (SMESG000051000.1)

HSP 1 Score: 1811.96 bits (4692), Expect = 0.000e+0
Identity = 939/946 (99.26%), Postives = 940/946 (99.37%), Query Frame = 3
Query:  126 MPRRXXXXXXXXXXXXXRESDQSSNNPQNDANVANISENIEANEVLAINNRQTVLNNTLIDEIENITISENVEKITSETPLPICEPSDAPKNNVSEPFKKFQNLNLNVDISKTMQMKFLPERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGLPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSRIYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEIIENLGKSVNEYFLSTHGINLKYPELLLIETTNYNKFPAELLTILPNQKVMGNDPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIKWALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXXXXXXXXXXXXXXXXXXXEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENESIGLPDISINEFHQNKMFFI 2963
            MPRRHKNKNSH+GSSNKRES QSSNNPQNDANVAN SENIEANEVLAINNRQTVLNNTLIDEIENITISENVEKITSETPLPICEPSDAPKNNVSEPFKKFQNLNLNVDISKTMQMKFLPERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGLPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSDIDLDLIDDYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSK LDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSRIYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEIIENLGKSVNEYFLSTHGINLKYPELLLIETTNYNKFPAELLTILPNQKVMGNDPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIKWALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDN FCYVSNDDIKKSIEKIKEDLDLLVVVVRNDVEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVE VVEMEDMV RLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENESIGLPDISINEFHQNKMFFI
Sbjct:    1 MPRRHKNKNSHRGSSNKRESGQSSNNPQNDANVANTSENIEANEVLAINNRQTVLNNTLIDEIENITISENVEKITSETPLPICEPSDAPKNNVSEPFKKFQNLNLNVDISKTMQMKFLPERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGLPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSDIDLDLIDDYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKPLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSRIYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEIIENLGKSVNEYFLSTHGINLKYPELLLIETTNYNKFPAELLTILPNQKVMGNDPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIKWALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNTFCYVSNDDIKKSIEKIKEDLDLLVVVVRNDVEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVETVVEMEDMVHRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENESIGLPDISINEFHQNKMFFI 946          
BLAST of Protein argonaute-3 vs. Planmine SMEST
Match: SMESG000050994.1 (SMESG000050994.1)

HSP 1 Score: 836.254 bits (2159), Expect = 0.000e+0
Identity = 451/957 (47.13%), Postives = 613/957 (64.05%), Query Frame = 3
Query:  192 SSNNPQNDANVANISEN----IEANEVLAINNRQTVLNNTLIDEIENITISENVEKITSETPLPICEPSDAPKNNVSEPFKKFQNLNLNVDISKTMQMKFLPERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVL-------ENNVRDFTNKIKRKDIFRDVVSQFLKDKCGLPIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXX-YIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNNFNIQNKKHINSRIYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEIIENLGKSVNEYFLSTHGINLKYPELLLIETT-NYNKF-PAELLTILPNQKVMGNDPNIVREVIKHSAIPCTERFDNLKKSVVAFK-GFRNNKYLQSYNITIIPQMINVKGRILDEPTALF-GDKEEKLPKSKFNIMNKKFKDPFHDPIKWALFAFGERREYG-NENELYAIEIELPKAAIKVNVKILKDNKFCYVSNXXXXXXXXXXXXXXXXXXXXXXXX-------XEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWE---IKNIEYAPIKGLFMVFGVDVTHP-SNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGRILKDDEDNISLSSENESIGLPDIS-----INEFHQNKMFFI 2963
            SS   + +A V   +E     IEA      ++ Q+     L  E E++TIS   ++  +  P+     S  P+  +S P K +  + L++ IS T+Q KFLP RPD GG KGR I LI+NC ++ +   KIFMYDIEL KAE+++       +   RD T K+K+KD+ R++V + L+   G+P+FDGGRIIF N+LLPY +   K+   +NYE++N+ CH N+Q V  +       + YI S KGRS+++PQE +R LD I REM L+ +LI IG + +F+P+TS  L   PY ++ GY +TIRPHM  +RFN D TQ+VCH+AIN+ DYI K+   N  N    +++  ++  ++V+A+H KN  G+ Y+R +T+ G   K+A  E I     ++ +YF   + I LKYP L +++T+ N   F P ELLT+LPNQ+V+ +D  IV+EVIK +AIP  +R   L  ++ + + G R +K+LQ+Y I I P++I V  R+LD P  +F G + E L   K++I NKKF  P+   + WALFA G  R+Y  N+  LY IE +LP AA  V +++     F YV   +I+K       + +     +           +YK VK  GD+  GIVTQC+ Y  L RQK L QY+GN+MLKVN KLGGVNWE   I+N  + PIK  FMVFG+DVTHP S  + LSKSVAA+VGS++  AT+YSA+I++QLSK  +  +EIV+EMEDMV  L+  FY  N+   P  LIFYRDGVSEGQF+IVF HEI AIQ AC+K +K+Y+P ITYI+VQKRH IRF P   +D VGR  NV  G V+D DITH REFDFYLCSHEGIQGTSKP HY+V+ DEN W S++LQ FTYFLC++  RCTRS+SYPAPT+YAHLAAFRGR L    D+ S S+ +      D S     INEFH +K+FFI
Sbjct:  302 SSQTTEENARVPRGAEGFSPIIEAPSKGLPHSIQSQPITPLTSEFESMTISSPKDEF-AVVPVAAKASSPPPQAKIS-PKKIYTPITLDIKISPTLQQKFLPSRPDDGGKKGRPIKLIINCCDVKINPMKIFMYDIELTKAEVIVAEKDKTEKKKSRDVTPKMKKKDVIRNIVKELLQKAEGMPVFDGGRIIFTNKLLPYNDGTCKITHNFNYESENVTCHFNIQRVKEEDIELGNLNNYIQSKKGRSDDMPQEALRALDTIFREMFLNKNLITIGGRAIFDPKTSTPLRCGPYILMEGYATTIRPHMWKVRFNFDKTQVVCHEAINLYDYISKIMDPNRLNPNTIRNLTRKLKTLRVQANHLKNADGKAYSRKYTIFGFSNKSAREEKIVEKNLTIEQYFQKEYNIKLKYPNLPMVKTSPNKPTFVPIELLTMLPNQRVIESDGKIVQEVIKFAAIPAADRLYKLNSAMSSLQIGNRGDKFLQNYKIEIDPKLIGVDARVLDGPEGIFGGQRTEPLRGGKWDIRNKKFIKPYDKLVNWALFAVGTERDYQRNDKPLYDIEDKLPGAANNVGIRMGGSFYFSYVPYQNIEKVATDTIRNSEKEKKKLDLLVFVVINDEDYKEVKKFGDVLFGIVTQCMKYATLNRQKFLDQYLGNIMLKVNGKLGGVNWELSPIRNRLFTPIKDSFMVFGIDVTHPASTRNDLSKSVAAMVGSLDNNATMYSALIRQQLSKKERAIIEIVMEMEDMVYNLLMTFYKANKQLKPKRLIFYRDGVSEGQFDIVFKHEIIAIQSACQKLEKDYQPPITYIVVQKRHKIRFFPQNEKDGVGRSGNVPSGTVVDKDITHCREFDFYLCSHEGIQGTSKPAHYHVLFDENNWGSDELQSFTYFLCHIYARCTRSVSYPAPTYYAHLAAFRGRTLIGLSDDDSSSTTSGHSTRTDTSESQILINEFHHDKLFFI 1256          
BLAST of Protein argonaute-3 vs. Planmine SMEST
Match: SMESG000047591.1 (SMESG000047591.1)

HSP 1 Score: 320.857 bits (821), Expect = 2.034e-93
Identity = 233/819 (28.45%), Postives = 399/819 (48.72%), Query Frame = 3
Query:  483 PERPDKGGTKGRQIPLIVNCFNMTVKSTKIFMYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGL---PIFDGGRIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSXXXXXXXXXYIHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLDRDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNN-----FNIQNKKHINSRIYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII-------ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNK---FPAELLTILPNQKVMGNDPNI-VREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQMINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHD-PIK-WALFAFGERREYGNENELYAIEIELPKAAIKVNVKILKDNKFC-YVSNXXXXXXXXXXXXXXXXXXXXXXXXXE-----YKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNW-EIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAHLAAFRGR 2855
            P RPD+G T GR I L  N F + +    +  YDI +   +     N          +DI + +V+     K      P+FDG R ++  + L   +   +L  I   E ++    + L+ V+      L +      +GR   IP ET+  LD+I+R  L       +G     N             +  G+  ++RP    +  N+D +    ++  ++ +++ +V  ++           +      I  +KV+ +H    +     R + V  V +++A ++         + +  +V +YF   +G+ L++P L  ++     K    P E+  I+  Q+ +    ++    +IK +A    +R   +  ++V    F  + +LQ + +++  +M +++GR++  P   +G + +     +  + + + K  F    IK WA+  F  +R    E+ L     +L K +    + I+    FC Y +  D  + + +  ++    + ++   +      Y  VK +GDI  G+ TQC+  +N+   K   Q + NL LK+N KLGG+N   + ++  A  K   +  G D THP   D    S+AAVVGS++   + Y+A +  Q  +      E + ++  MV+ L+  FY   + K P  +I+YRDGVSEGQF  V NHE+ AI+ AC K + +Y+P IT+++VQKRH+ R      +D +GR  N+  G  +D  ITH  EFDFYLCSH GIQGTS+P HY+V+ D+NR++++++Q  TY LC+   RCTRS+S PAP +YAHL AFR R
Sbjct:   96 PSRPDQG-TDGRTIQLKANHFEIVMPKGFLHHYDITITPEKCPRRVN----------RDIIQAMVNNMHYQKYFYNQKPVFDGRRNMYTREPLSIGKEKLELEVILPGEGKDRVFKVCLKHVSEVSLFALEEAL----QGRRQTIPAETVTALDVIMRH-LPSMTYTPVGRSFFHNTGDYDNPLGGGREVWFGFHQSVRPSQWKMMLNIDVSATAFYKEQSVINFMFEVLEIDKDQKRPLTDSQRVKFTKEIKGLKVEINHCGPIK-----RKYRVCNVTRRSAQSQTFPLQSEQGQTIECTVTKYFQDRYGMKLQHPHLPCLQVGQEQKHTYLPLEVCNIVSGQRCIKKLTDMQTSTMIKATARSAPDREKEIN-NLVRRSNFNADPHLQIFGVSVNTRMADIQGRVIPAPKIQYGGRTKAQATPQQGVWDMRGKQFFSGIEIKVWAIACFAPQRTV-REDSLRTFTQQLQKISNDAGMPIVGQPCFCKYATGQDQVEPMFRYLKNSFTGLQLIVVVLPGKTPVYAEVKRVGDIIFGLATQCVQAKNV--NKPSPQTLSNLCLKINVKLGGINSILVPSVRPAVFKEPVIFLGADFTHPPAGDKTKPSIAAVVGSMDAHPSRYAATVSVQPHRQ-----ETIQDLAAMVKELLIQFYKSTRFK-PTRIIYYRDGVSEGQFLNVLNHELKAIREACIKLEMSYQPGITFVVVQKRHHTRLFCSDKKDQMGRSGNIPAGTTVDQIITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSADEIQTLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRAR 883          
BLAST of Protein argonaute-3 vs. Planmine SMEST
Match: SMESG000081970.1 (SMESG000081970.1)

HSP 1 Score: 159.844 bits (403), Expect = 1.848e-39
Identity = 102/303 (33.66%), Postives = 159/303 (52.48%), Query Frame = 3
Query: 1977 YKLVKDIGDIELGIVTQCICYENLCRQKNLSQYMGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTHYAH-LAAFRGRILKDDEDNI 2882
            Y  +K    ++  + +Q +  + L     L+    N+ L++NAKLGG  W +K     PIK  FMV G+DV H  +  G S+SV AVVGS N   T + +  K  L +  K   EI+  ++  +  L+K +++ N   LP+ +  YRDGVS+GQ   V   EI       E F  +YKP + Y +VQKR N R   ++   + G+  N  PG ++D+ +T +  +DF+L S    QGT  P HY V+ D +++  + +Q   Y  C++ +    ++  PAP  YAH LA   G+ LK +  N+
Sbjct:  678 YNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVK----IPIKK-FMVVGLDVWH--DTKGRSRSVGAVVGSTNALCTRWFS--KSHLQEQDK---EIMYVLQSCMLSLLKAYFEENNF-LPETIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLY-VSDPKNKGQINNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSKYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMVGQNLKAEPSNL 966          
BLAST of Protein argonaute-3 vs. Planmine SMEST
Match: SMESG000069984.1 (SMESG000069984.1)

HSP 1 Score: 111.309 bits (277), Expect = 1.659e-24
Identity = 90/335 (26.87%), Postives = 152/335 (45.37%), Query Frame = 3
Query: 2004 IELGIVTQCICYENLCRQKNLSQYM--GNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKSVAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYDLNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYK---PKITYIIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHEGIQGTSKPVHYYVILDENRWNS---------NDLQEFTYFLCYVCFRCTRSISYPAPTHYAH-LAAFRGRILKDDEDNISLSSENESIGLPDISINEFHQNKMFFI 2963
            +  G++TQC+   N   + +  + +     ++++ AK G   W ++ I+  P     M+ G+D  H   N    KS+ A V SIN T T Y + +     K  ++  E + +  ++     K  YD+    LP  ++ +RDGV + Q +   N E+ A++   E   K      P+I Y+IV+KR   +           R  N  PG V+D +I     ++F+L S    +GT+ P +Y V+ D               N+LQ+ TY L ++ F    +I  P P HYAH LA   G++ K               G   ++INE  +N++F++
Sbjct:  539 MSTGLLTQCVTTRNGTNRNDKRRKVVSSKTVMQIFAKFGYDPWTVE-IKLRPT----MIVGMDTYH---NKSSKKSIQASVFSINSTFTQYMSFVNS--PKGRQEFHETLGKNFNLALEDFKKRYDI----LPQRILVFRDGVGDNQLQFTKNFEVDAMKPLIENIYKGNGFPVPQIIYVIVKKRVGTKL--------FNRGNNPNPGTVVDKEIVKPNFYEFFLVSQRTTKGTATPTNYNVLEDTRLLTKKGTMDPMAPNELQKITYALTHLYFNWMGTIRVPVPVHYAHRLAELVGKVHK---------------GSNPVAINERIRNRLFYL 836          
The following BLAST results are available for this feature:
BLAST of Protein argonaute-3 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
AGO24.993e-10529.47argonaute RISC catalytic component 2 [Source:HGNC ... [more]
AGO33.698e-9627.68argonaute RISC catalytic component 3 [Source:HGNC ... [more]
AGO44.856e-9228.45argonaute RISC component 4 [Source:HGNC Symbol;Acc... [more]
AGO14.145e-8927.56argonaute RISC component 1 [Source:HGNC Symbol;Acc... [more]
AGO31.094e-8733.09argonaute RISC catalytic component 3 [Source:HGNC ... [more]
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BLAST of Protein argonaute-3 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
alg-23.478e-9628.34pep chromosome:WBcel235:II:874160:879001:1 gene:WB... [more]
alg-27.804e-9628.50pep chromosome:WBcel235:II:873176:878945:1 gene:WB... [more]
alg-11.842e-9327.76pep chromosome:WBcel235:X:13951262:13956261:1 gene... [more]
alg-13.057e-9327.76pep chromosome:WBcel235:X:13949114:13956261:1 gene... [more]
alg-43.974e-6331.35pep chromosome:WBcel235:III:9887211:9891355:-1 gen... [more]
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BLAST of Protein argonaute-3 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
AGO11.435e-9329.22gene:FBgn0262739 transcript:FBtr0305592[more]
AGO11.435e-9329.22gene:FBgn0262739 transcript:FBtr0087612[more]
AGO11.435e-9329.22gene:FBgn0262739 transcript:FBtr0087613[more]
AGO11.846e-9329.22gene:FBgn0262739 transcript:FBtr0087614[more]
AGO21.165e-5530.91gene:FBgn0087035 transcript:FBtr0331933[more]
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BLAST of Protein argonaute-3 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ago21.320e-10129.14argonaute RISC catalytic component 2 [Source:ZFIN;... [more]
ago3b2.782e-9727.71argonaute RISC catalytic component 3b [Source:NCBI... [more]
ago48.479e-9729.28argonaute RISC component 4 [Source:ZFIN;Acc:ZDB-GE... [more]
ago3b1.345e-9527.71argonaute RISC catalytic component 3b [Source:NCBI... [more]
ago3a3.286e-9327.24argonaute RISC catalytic component 3a [Source:ZFIN... [more]
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BLAST of Protein argonaute-3 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rbm263.836e-10429.35RNA binding motif protein 26 [Source:Xenbase;Acc:X... [more]
rbm261.241e-10329.39RNA binding motif protein 26 [Source:Xenbase;Acc:X... [more]
rbm263.456e-10329.35RNA binding motif protein 26 [Source:Xenbase;Acc:X... [more]
rbm261.344e-10229.12RNA binding motif protein 26 [Source:Xenbase;Acc:X... [more]
rbm266.313e-10229.29RNA binding motif protein 26 [Source:Xenbase;Acc:X... [more]
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BLAST of Protein argonaute-3 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ago23.199e-10529.47argonaute RISC catalytic subunit 2 [Source:MGI Sym... [more]
Ago33.294e-9627.59argonaute RISC catalytic subunit 3 [Source:MGI Sym... [more]
Ago42.998e-9128.22argonaute RISC catalytic subunit 4 [Source:MGI Sym... [more]
Ago12.846e-8927.56argonaute RISC catalytic subunit 1 [Source:MGI Sym... [more]
Ago17.078e-7932.88argonaute RISC catalytic subunit 1 [Source:MGI Sym... [more]
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BLAST of Protein argonaute-3 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|O77503|AGO2_RABIT1.134e-10429.47Protein argonaute-2 (Fragment) OS=Oryctolagus cuni... [more]
sp|Q6QME8|AGO2_BOVIN1.941e-10429.47Protein argonaute-2 OS=Bos taurus OX=9913 GN=AGO2 ... [more]
sp|Q8CJG0|AGO2_MOUSE2.237e-10429.47Protein argonaute-2 OS=Mus musculus OX=10090 GN=Ag... [more]
sp|Q9UKV8|AGO2_HUMAN2.398e-10429.47Protein argonaute-2 OS=Homo sapiens OX=9606 GN=AGO... [more]
sp|Q9QZ81|AGO2_RAT3.127e-10429.47Protein argonaute-2 OS=Rattus norvegicus OX=10116 ... [more]
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BLAST of Protein argonaute-3 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0X3NYE93.747e-14637.03Protein argonaute-3 (Fragment) OS=Schistocephalus ... [more]
A0A183TST33.219e-14436.56Uncharacterized protein OS=Schistocephalus solidus... [more]
A0A3P7DMT73.729e-14436.56Uncharacterized protein OS=Schistocephalus solidus... [more]
A0A3P6Q1W23.921e-14236.24Uncharacterized protein OS=Dibothriocephalus latus... [more]
A0A068WVW11.170e-13534.07Eukaryotic translation initiation factor 2c OS=Ech... [more]
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BLAST of Protein argonaute-3 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ago21.625e-10229.26argonaute RISC catalytic component 2 [Source:NCBI ... [more]
ago21.693e-10229.26argonaute RISC catalytic component 2 [Source:NCBI ... [more]
ago41.530e-9429.14protein argonaute-4 [Source:NCBI gene;Acc:10304381... [more]
ago43.842e-9129.62protein argonaute-4 [Source:NCBI gene;Acc:10304381... [more]
ago12.169e-8730.47argonaute RISC component 1 [Source:ZFIN;Acc:ZDB-GE... [more]
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BLAST of Protein argonaute-3 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ago29.201e-6645.34argonaute RISC catalytic component 2 [Source:ZFIN;... [more]
ENSPMAT00000009610.13.283e-5726.15pep scaffold:Pmarinus_7.0:GL477777:44890:66775:1 g... [more]
piwil23.565e-3733.88piwi-like RNA-mediated gene silencing 2 [Source:NC... [more]
piwil11.271e-2128.08piwi-like RNA-mediated gene silencing 1 [Source:NC... [more]
ENSPMAT00000009316.12.836e-1119.64pep scaffold:Pmarinus_7.0:GL484064:2408:13690:1 ge... [more]
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BLAST of Protein argonaute-3 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Protein argonaute-3 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO464312.038e-10030.19Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO385831.879e-3938.21Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO340273.445e-3530.14Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO499312.450e-3429.73Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO281243.926e-1038.24Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Protein argonaute-3 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
AGO24.414e-10329.52protein argonaute-2 [Source:NCBI gene;Acc:10115610... [more]
ENSORLT00000011404.23.679e-9528.05protein argonaute-3 [Source:NCBI gene;Acc:10116565... [more]
AGO27.186e-9528.34protein argonaute-2 [Source:NCBI gene;Acc:10115610... [more]
ago41.900e-9328.90protein argonaute-4 [Source:NCBI gene;Acc:10116746... [more]
ago3a1.509e-9028.31protein argonaute-3 [Source:NCBI gene;Acc:10115732... [more]
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BLAST of Protein argonaute-3 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000051000.10.000e+099.26SMESG000051000.1[more]
SMESG000050994.10.000e+047.13SMESG000050994.1[more]
SMESG000047591.12.034e-9328.45SMESG000047591.1[more]
SMESG000081970.11.848e-3933.66SMESG000081970.1[more]
SMESG000069984.11.659e-2426.87SMESG000069984.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024072 ID=SMED30024072|Name=Protein argonaute-3|organism=Schmidtea mediterranea sexual|type=transcript|length=3458bp
TATTTTTAAATAAATTATTTCTAAGAATAAATATATTTCTAAGAATAAAT
CTATTAATAATTCTATTTTTTCAAATAATTTGATTTTAATTTTTGTTTTT
AGTTTGTTAATTTCTACACTTAAAAATGCCTAGACGCCATAAAAACAAAA
ATTCACACAAAGGAAGCTCTAACAAGCGAGAAAGTGATCAATCTTCTAAT
AATCCGCAAAATGATGCAAATGTAGCGAATATATCTGAAAATATTGAAGC
AAATGAGGTTTTAGCAATAAATAATAGACAAACTGTATTAAATAATACTT
TAATTGATGAAATTGAAAATATTACGATTTCTGAAAACGTCGAAAAAATT
ACCAGTGAAACACCATTACCCATATGTGAACCAAGCGATGCTCCTAAAAA
TAATGTCTCTGAACCATTTAAGAAATTCCAAAATCTTAATCTCAATGTTG
ATATTTCTAAAACAATGCAAATGAAGTTTTTACCCGAACGACCAGATAAA
GGTGGTACTAAAGGTCGACAAATTCCGTTAATCGTTAATTGTTTCAATAT
GACTGTTAAGTCTACAAAAATTTTTATGTACGATATAGAATTGCTTAAAG
CAGAGTTGGTGTTGGAAAATAATGTTAGAGATTTTACTAACAAAATAAAA
CGAAAAGATATTTTCCGCGATGTCGTTTCACAGTTTTTAAAAGATAAATG
TGGATTACCAATTTTTGATGGTGGACGAATTATTTTCAACAATCAGTTAT
TGCCTTATACAGAAACTATTAATAAATTGAACTTTATTTACAATTATGAA
AACCAAAATATTAATTGCCATCTAAATCTACAACTTGTGAACAGTGATAT
AGATCTGGATTTGATCGACGATTACATTCATTCTGGGAAAGGACGCAGTA
ATAATATTCCCCAGGAAACCATAAGAGTTTTGGATATCATTATTAGAGAA
ATGCTTCTCGATTGTGATTTAATTCTCATCGGAAACAAAGGCTTATTTAA
TCCAAGAACTTCCAAGCAACTCGACAGAGATCCATATACCATTTTGTCGG
GATATTTCTCTACAATTCGGCCTCATATGTCTAATATAAGATTTAATCTA
GATACAACACAAATCGTTTGTCATCAAGCTATTAATATGAAGGATTACAT
TCAAAAAGTTTTTACTTTAAATAATTTCAACATCCAAAATAAAAAACATA
TAAATAGCCGAATTTACAATATAAAAGTGAAAGCGTCTCATTATAAAAAT
GACCAAGGTGAAAATTACACTAGAATATTTACTGTTAAAGGCGTTTATCA
AAAAAATGCTCATAATGAAATAATTGAAAATTTGGGGAAAAGTGTAAATG
AATATTTTCTTTCTACGCATGGTATAAATTTAAAATATCCTGAACTTTTA
TTAATTGAAACAACTAATTACAATAAATTTCCCGCCGAACTATTAACAAT
TTTACCAAATCAAAAAGTTATGGGAAACGATCCAAATATCGTTAGGGAGG
TCATCAAACATTCTGCAATTCCATGTACTGAGAGATTTGATAATTTAAAA
AAATCAGTTGTGGCATTTAAAGGATTTCGAAATAACAAATACTTACAAAG
TTACAATATTACAATAATTCCACAGATGATTAATGTTAAAGGGAGAATTC
TTGATGAGCCTACTGCTTTATTCGGTGATAAGGAAGAAAAGCTGCCTAAA
TCTAAATTTAATATAATGAACAAAAAATTCAAAGATCCCTTTCATGATCC
CATTAAATGGGCATTATTTGCATTTGGTGAACGCAGAGAATATGGAAATG
AAAATGAACTTTATGCTATAGAAATTGAACTTCCTAAAGCAGCAATTAAA
GTTAATGTCAAGATACTTAAAGATAATAAATTCTGTTACGTGTCAAATGA
TGACATTAAAAAATCCATAGAAAAGATTAAAGAAGATTTAGATTTATTAG
TTGTTGTAGTGAGAAATGATGTAGAATATAAACTTGTAAAGGATATTGGT
GACATAGAACTAGGAATTGTAACCCAATGTATATGTTATGAAAATTTATG
TAGACAAAAGAATTTATCTCAATATATGGGAAATCTCATGTTAAAAGTTA
ACGCTAAGCTTGGTGGTGTAAATTGGGAGATTAAAAACATAGAATATGCC
CCCATTAAAGGTTTATTTATGGTGTTTGGAGTAGATGTTACACATCCTTC
CAATAATGACGGTCTTTCTAAAAGTGTGGCAGCGGTTGTTGGAAGTATAA
ATAAAACTGCTACTATATATAGTGCAGTTATAAAGAAACAGCTGTCTAAA
GACGGTAAGGATGCTGTAGAAATTGTGGTTGAAATGGAAGACATGGTTCA
GCGATTGATAAAGGTATTTTACGATTTAAATCAATCAAAGTTACCGGATC
ATCTCATATTTTATCGTGATGGAGTTTCAGAGGGTCAGTTTGAAATAGTT
TTCAACCATGAAATTGCCGCCATTCAATATGCATGTGAAAAGTTTAAGAA
AAACTACAAACCTAAAATTACTTATATAATCGTTCAAAAGCGACACAATA
TTCGATTTTGTCCAATTACTTTAGAGGATTCGGTTGGAAGATGTAAAAAT
GTTCTTCCCGGTGTTGTGATTGATAATGATATCACCCATTCTAGAGAATT
CGATTTTTATCTATGTTCTCACGAGGGTATACAGGGAACATCAAAGCCCG
TTCATTATTATGTGATACTTGATGAAAACAGGTGGAATTCAAACGATCTT
CAAGAATTCACCTATTTCTTGTGTTATGTTTGTTTTCGATGTACTAGAAG
TATTTCTTATCCAGCACCAACCCATTACGCCCATTTAGCGGCATTTAGAG
GTAGAATACTGAAAGACGATGAAGATAATATATCGCTTTCGTCGGAAAAT
GAAAGTATCGGCTTGCCTGATATTTCTATTAATGAATTTCATCAAAACAA
AATGTTCTTCATCTAACTATGGCATGTTTTCATAACGGTTGTTAGCTTTA
CATCATTGATTCTCTATCATAACAATAATTTGTTTATTCAAACCCCTATT
TTAATCGTTGAATATTTTTCAGCCATTTCAAACACTAATTTAAGTGTTCT
GAATTGATCAATGACTATCTCTTTTGGAAATTTATAATTATGATCTTAAT
TAACGGTACGGAACATTTAGTGTTTTTGAAACTGTAATGGAGCACTTCTC
TTAACAGTTATTTTTAAATGCATTATTGATTCGTAAGTGCGATTTTCAAA
AAATTGATAAGAAGTTTACAGTAAGGAATCGATACGATAACCGAAGTAAT
TTCCAGGGCTATGTTAAAATATCGATTTAAATGATGTTTTACATATTTTC
CGTGATTGTTTATCAATAGCTGTTGACTAGTTCGCGATAAATAAAGATTT
TCACTAAACACAAACTTTGGAATAATTCATTGGAAATTCATTGCCAACAA
TTCGTTGA
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protein sequence of SMED30024072-orf-1

>SMED30024072-orf-1 ID=SMED30024072-orf-1|Name=SMED30024072-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=947bp
MPRRHKNKNSHKGSSNKRESDQSSNNPQNDANVANISENIEANEVLAINN
RQTVLNNTLIDEIENITISENVEKITSETPLPICEPSDAPKNNVSEPFKK
FQNLNLNVDISKTMQMKFLPERPDKGGTKGRQIPLIVNCFNMTVKSTKIF
MYDIELLKAELVLENNVRDFTNKIKRKDIFRDVVSQFLKDKCGLPIFDGG
RIIFNNQLLPYTETINKLNFIYNYENQNINCHLNLQLVNSDIDLDLIDDY
IHSGKGRSNNIPQETIRVLDIIIREMLLDCDLILIGNKGLFNPRTSKQLD
RDPYTILSGYFSTIRPHMSNIRFNLDTTQIVCHQAINMKDYIQKVFTLNN
FNIQNKKHINSRIYNIKVKASHYKNDQGENYTRIFTVKGVYQKNAHNEII
ENLGKSVNEYFLSTHGINLKYPELLLIETTNYNKFPAELLTILPNQKVMG
NDPNIVREVIKHSAIPCTERFDNLKKSVVAFKGFRNNKYLQSYNITIIPQ
MINVKGRILDEPTALFGDKEEKLPKSKFNIMNKKFKDPFHDPIKWALFAF
GERREYGNENELYAIEIELPKAAIKVNVKILKDNKFCYVSNDDIKKSIEK
IKEDLDLLVVVVRNDVEYKLVKDIGDIELGIVTQCICYENLCRQKNLSQY
MGNLMLKVNAKLGGVNWEIKNIEYAPIKGLFMVFGVDVTHPSNNDGLSKS
VAAVVGSINKTATIYSAVIKKQLSKDGKDAVEIVVEMEDMVQRLIKVFYD
LNQSKLPDHLIFYRDGVSEGQFEIVFNHEIAAIQYACEKFKKNYKPKITY
IIVQKRHNIRFCPITLEDSVGRCKNVLPGVVIDNDITHSREFDFYLCSHE
GIQGTSKPVHYYVILDENRWNSNDLQEFTYFLCYVCFRCTRSISYPAPTH
YAHLAAFRGRILKDDEDNISLSSENESIGLPDISINEFHQNKMFFI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0031047gene silencing by RNA
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR036397RNaseH_sf
IPR036085PAZ_dom_sf
IPR003165Piwi
IPR032474Argonaute_N
IPR012337RNaseH-like_sf
IPR032472ArgoL2
IPR003100PAZ_dom
Vocabulary: molecular function
TermDefinition
GO:0003676nucleic acid binding
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003165Piwi domainSMARTSM00950Piwi_a_2coord: 607..914
e-value: 1.9E-100
score: 349.7
IPR003165Piwi domainPFAMPF02171Piwicoord: 613..910
e-value: 1.2E-84
score: 284.2
IPR003165Piwi domainPROSITEPS50822PIWIcoord: 607..907
score: 38.475
NoneNo IPR availableGENE3DG3DSA:2.170.260.10coord: 331..449
e-value: 3.4E-16
score: 61.5
NoneNo IPR availableGENE3DG3DSA:3.40.50.2300coord: 508..671
e-value: 5.8E-25
score: 89.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..29
NoneNo IPR availablePANTHERPTHR22891EUKARYOTIC TRANSLATION INITIATION FACTOR 2Ccoord: 127..930
NoneNo IPR availableCDDcd02846PAZ_argonaute_likecoord: 335..443
e-value: 1.78246E-10
score: 56.9418
NoneNo IPR availableCDDcd04657Piwi_ago-likecoord: 488..910
e-value: 8.46284E-149
score: 445.519
IPR032472Argonaute linker 2 domainPFAMPF16488ArgoL2coord: 469..513
e-value: 2.3E-6
score: 27.8
IPR032474Protein argonaute, N-terminalPFAMPF16486ArgoNcoord: 131..273
e-value: 8.8E-12
score: 45.8
IPR036397Ribonuclease H superfamilyGENE3DG3DSA:3.30.420.10coord: 677..946
e-value: 8.8E-97
score: 325.5
IPR003100PAZ domainPROSITEPS50821PAZcoord: 406..444
score: 10.735
IPR036085PAZ domain superfamilySUPERFAMILYSSF101690PAZ domaincoord: 332..477
IPR012337Ribonuclease H-like superfamilySUPERFAMILYSSF53098Ribonuclease H-likecoord: 600..914