SMED30024068
Overview
Neoblast Clusters
Zeng et. al., 2018
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppEmbryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30024068 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Homology
BLAST of SMED30024068 vs. TrEMBL
Match: A0A2G8LCX0 (Integrase catalytic domain-containing protein OS=Stichopus japonicus OX=307972 GN=BSL78_04960 PE=4 SV=1) HSP 1 Score: 51.9878 bits (123), Expect = 2.619e-9 Identity = 38/91 (41.76%), Postives = 50/91 (54.95%), Query Frame = -1 Query: 266 RYL--FVCFKSKELHLE*ATGLDTESSXXXXXXXXXXXRMPKTISSDNGKNFEGTNNHMENIW--IDNYRTERVL*KNEL--QCIFNPPAA 520 RYL F C K++ +HLE A GLDT+S L R +RR +P I SDNG NF G N ++ ID + ER K + + + NPP A Sbjct: 135 RYLCLFTCMKTRAVHLEMAFGLDTDSFINCLYRFTSRRGIPSEIRSDNGTNFVGARNEFIKLFDTIDKAKVERDTSKKGINWKWVLNPPLA 225 HSP 2 Score: 38.5058 bits (88), Expect = 2.619e-9 Identity = 32/114 (28.07%), Postives = 53/114 (46.49%), Query Frame = -3 Query: 501 LIFSDRSWISKLIVKDFHKKKHQYAG------VLDKIFWLLRAIQIIQVIENQRVGYCRQKAKGST*LMAPRSQFRF-AKPLRAFAQMK*ILLVNFKPKQTKVKVS----LCMF 809 +I + +++LIVK+ H++ AG +L +WL+ + I+ EN R K +G +MAP + R LRAF ++ F KQ + K+ LC+F Sbjct: 27 IILPRKHPVTRLIVKEHHEEGKHIAGTNHTLALLSNKYWLIGGREEIREWENACNECKRGKPRGGQQIMAPLPKSRTNVDTLRAFDKVSVDFAGPFLTKQGRGKIKTKRYLCLF 140
BLAST of SMED30024068 vs. TrEMBL
Match: A0A2B4RBS5 (Uncharacterized protein OS=Stylophora pistillata OX=50429 GN=AWC38_SpisGene22069 PE=4 SV=1) HSP 1 Score: 45.8246 bits (107), Expect = 3.234e-9 Identity = 26/86 (30.23%), Postives = 47/86 (54.65%), Query Frame = -3 Query: 570 LIFSDRSWISKLIVKDFHKK------KHQYAGVLDKIFWLLRAIQIIQVIENQRVGYCRQKAKGST*LMAPRSQFRFAKPLRAFAQ 809 +I + W++KLIV+ +H++ HQ +L FWL++ + I+ + + R+KAK + +MAP + R P RAF++ Sbjct: 381 IILPRKEWVTKLIVRWYHQQGNHPSGTHQTLAMLSSRFWLMQRREEIRECKGECYECRRRKAKAAQQVMAPLPKIRLKMPPRAFSR 466 HSP 2 Score: 44.2838 bits (103), Expect = 3.234e-9 Identity = 30/66 (45.45%), Postives = 41/66 (62.12%), Query Frame = -1 Query: 341 RQR*RYL--FVCFKSKELHLE*ATGLDTESSXXXXXXXXXXXRMPKTISSDNGKNFEGTNNHMENI 532 R+ RYL F C S+ LHLE A GLDT+S A R+A+RR +P+ + SDNG NF G + + + Sbjct: 484 RRAKRYLCLFTCLTSRALHLEIAYGLDTDSFLNAFCRMASRRGLPEEMISDNGNNFVGADLELREL 549
BLAST of SMED30024068 vs. TrEMBL
Match: A0A2G8KMP0 (Integrase_H2C2 domain-containing protein OS=Stichopus japonicus OX=307972 GN=BSL78_13912 PE=4 SV=1) HSP 1 Score: 48.9062 bits (115), Expect = 7.791e-8 Identity = 31/69 (44.93%), Postives = 40/69 (57.97%), Query Frame = -1 Query: 314 LFVCFKSKELHLE*ATGLDTESSXXXXXXXXXXXRMPKTISSDNGKNFEGTNNHMENIW--IDNYRTER 514 LF C K++ +HLE A GLDT+S L R +RR +P I SDNG NF G N ++ ID + ER Sbjct: 139 LFTCMKTRAVHLEMAFGLDTDSFINCLYRFTSRRGIPSEIRSDNGTNFVGARNEFIKLFDTIDKAKVER 207 HSP 2 Score: 36.5798 bits (83), Expect = 7.791e-8 Identity = 31/114 (27.19%), Postives = 52/114 (45.61%), Query Frame = -3 Query: 501 LIFSDRSWISKLIVKDFHKKKHQYAG------VLDKIFWLLRAIQIIQVIENQRVGYCRQKAKGST*LMAPRSQFRF-AKPLRAFAQMK*ILLVNFKPKQTKVKVS----LCMF 809 +I + +++LIVK+ H + AG +L +WL+ + I+ EN R K +G +MAP + R +RAF ++ F KQ + K+ LC+F Sbjct: 27 IILPRKHPVTRLIVKEHHVEGKHIAGTNHTLALLSNKYWLIGGREEIREWENTCNECKRGKPRGGQQIMAPLPKSRTNVDTMRAFDKVSVDFAGQFLTKQGRGKIKTKRYLCLF 140
BLAST of SMED30024068 vs. TrEMBL
Match: A0A0S7GQ49 (PPUP5170 (Fragment) OS=Poeciliopsis prolifica OX=188132 GN=PPUP5170 PE=4 SV=1) HSP 1 Score: 54.299 bits (129), Expect = 1.275e-7 Identity = 38/91 (41.76%), Postives = 51/91 (56.04%), Query Frame = -1 Query: 263 RY--LFVCFKSKELHLE*ATGLDTESSXXXXXXXXXXXRMPKTISSDNGKNFEGTNNHMENIWIDNYRTERV---L*KNELQCIFNPPAAS 520 RY LF C ++ +HLE A LDTES AL R RR P+ I SDNG NF G + + ++ + +++ L + E Q FNPPAAS Sbjct: 353 RYGCLFTCLTTRAVHLEVAHSLDTESFINALQRFMARRGTPQLIRSDNGTNFVGARHELRKA-LEGWNQQKIEDHLLQRETQWKFNPPAAS 442 HSP 2 Score: 30.4166 bits (67), Expect = 1.275e-7 Identity = 31/103 (30.10%), Postives = 49/103 (47.57%), Query Frame = -3 Query: 501 ISKLIVKDFHKKKHQYAGV------LDKIFWLLRAIQIIQVIENQRVGYCRQKAKGST*LMA--PRSQFRFAKPLRAFAQMK*ILLVNFKPKQTKVKVSLCMF 785 I+KLI+ FHK ++GV + + FW++R ++ + Q V RQKA ST MA P + KP A + + K +++VK C+F Sbjct: 257 ITKLIIWHFHKLT-GHSGVERVLADIRQFFWIVRGRASVKQVLAQCVPCKRQKAPISTQYMADLPIDRVTPCKPPFAHVGIDYFGPITIKRGRSEVKRYGCLF 358
BLAST of SMED30024068 vs. TrEMBL
Match: A0A2B4RBG6 (Nucleotide-binding oligomerization domain-containing protein 2 OS=Stylophora pistillata OX=50429 GN=Nod2 PE=4 SV=1) HSP 1 Score: 45.4394 bits (106), Expect = 2.416e-6 Identity = 29/86 (33.72%), Postives = 44/86 (51.16%), Query Frame = -3 Query: 570 LIFSDRSWISKLIVKDFHKK------KHQYAGVLDKIFWLLRAIQIIQVIENQRVGYCRQKAKGST*LMAPRSQFRFAKPLRAFAQ 809 +I +S+++KLI K +H+K +Q L FW++ + I E + R+KAK + +MAP Q R LRAFAQ Sbjct: 1603 IILPRKSYVTKLIDKHYHEKDDHAGGTNQLLAALSTRFWIISGREEICEWEKECNECRRRKAKAAKQIMAPLPQIRLRYSLRAFAQ 1688 HSP 2 Score: 35.039 bits (79), Expect = 2.416e-6 Identity = 26/85 (30.59%), Postives = 44/85 (51.76%), Query Frame = -1 Query: 266 LFVCFKSKELHLE*ATGLDTESSXXXXXXXXXXXRMPKTISSDNGKNFEGTNNHMENI--WIDNYRTERVL*KNELQCIFNPPAA 514 LF C S+ +++E A LDT++ A R+ NRR +P+ + DNG N G N + ++ +D + + ++ NPP A Sbjct: 1714 LFTCLASRAVYVEMAYALDTDAFLNAFYRMVNRRGLPREMLCDNGGNLVGGNRELSDLVKELDQEKIAKSTANQGIKWQSNPPHA 1798
BLAST of SMED30024068 vs. Planmine SMEST
Match: SMESG000000866.1 (SMESG000000866.1) HSP 1 Score: 77.0258 bits (188), Expect = 3.573e-27 Identity = 38/89 (42.70%), Postives = 61/89 (68.54%), Query Frame = -1 Query: 263 QR*RYLFVCFKSKELHLE*ATGLDTESSXXXXXXXXXXXRMPKTISSDNGKNFEGTNNHMENIWIDNYRTERVL*KNELQCIFNPPAAS 529 +R +LFVC K++ +H+E A LDT++ +A +R + + MP+ + SDNG NF G +H+ NI ID+Y E+ L ++E + +FNPP+AS Sbjct: 158 KRYMFLFVCLKTRAVHIELAYKLDTDAFIKAFLRFTSLQEMPQLMLSDNGTNFVGAASHLRNIVIDSYMVEKELSRHECRWLFNPPSAS 246 HSP 2 Score: 63.1586 bits (152), Expect = 3.573e-27 Identity = 36/87 (41.38%), Postives = 55/87 (63.22%), Query Frame = -3 Query: 570 LIFSDRSWISKLIVKDFHKKKHQYAGV------LDKIFWLLRAIQIIQVIENQRVGYCRQ-KAKGST*LMAPRSQFRFAKPLRAFAQ 809 +I SD+S I+KLI++D ++ + YAGV L++ +W+L+A ++I E+ G CRQ KAKG T +MA FR ++PLR F Sbjct: 52 IILSDKSLIAKLIIRDIYESLNHYAGVQHVLSALNEKYWILKARKLIINCESSCKG-CRQRKAKGMTQMMATLPSFRLSEPLRGFVH 137
BLAST of SMED30024068 vs. Planmine SMEST
Match: SMESG000041215.1 (SMESG000041215.1) HSP 1 Score: 65.4698 bits (158), Expect = 5.160e-24 Identity = 35/87 (40.23%), Postives = 54/87 (62.07%), Query Frame = -3 Query: 567 LIFSDRSWISKLIVKDFHKKKHQYAGV------LDKIFWLLRAIQIIQVIENQRVGYCRQKAKGST*LMAPRSQFRFAKPLRAFAQM 809 +I SD SWI++LI+K+ H K YAGV L++ +W++ A ++I+ R+KAKG +MAP FRF +PL+AFA++ Sbjct: 1262 IILSDESWIARLIIKELHVKNKDYAGVNHTLGELNREYWVVCARKLIKKCMKGCFECRRRKAKGMEQIMAPLPSFRFQEPLQAFARI 1348 HSP 2 Score: 63.929 bits (154), Expect = 5.160e-24 Identity = 41/94 (43.62%), Postives = 58/94 (61.70%), Query Frame = -1 Query: 266 NQSRQR*RY--LFVCFKSKELHLE*ATGLDTESSXXXXXXXXXXXRMPKTISSDNGKNFEGTNNHMENIWIDNYRTERVL*KNELQCIFNPPAA 541 ++RQ+ RY LF C +++ +HLE A LDT++ RA IR RR P I SDNG NF G N ++ I +D+YR + + N++ IFNPP A Sbjct: 1363 GKTRQK-RYMCLFTCLQTRAVHLEVAHALDTDAFIRAFIRFMGRREKPLMILSDNGTNFVGATNKVQEIKVDSYRFNKFMSGNDIIWIFNPPLA 1455
BLAST of SMED30024068 vs. Planmine SMEST
Match: SMESG000043416.1 (SMESG000043416.1) HSP 1 Score: 64.6994 bits (156), Expect = 2.569e-20 Identity = 35/87 (40.23%), Postives = 55/87 (63.22%), Query Frame = -3 Query: 567 LIFSDRSWISKLIVKDFHKKKHQYAGV------LDKIFWLLRAIQIIQVIENQRVGYCRQKAKGST*LMAPRSQFRFAKPLRAFAQM 809 +I SD SWI++LI+K+ H K YAGV L++ +W++ A ++I+ + R+KAKG +MAP FRF +PL+AFA++ Sbjct: 128 IILSDESWIARLIIKELHVKNKHYAGVNHTLGKLNRKYWVVCARKLIKKCIKRCFECRRRKAKGMEQIMAPLPSFRFQEPLQAFARI 214 HSP 2 Score: 51.9878 bits (123), Expect = 2.569e-20 Identity = 33/81 (40.74%), Postives = 46/81 (56.79%), Query Frame = -1 Query: 266 VCFKSKELHLE*ATGLDTESSXXXXXXXXXXXRMPKTISSDNGKNFEGTNNHMENIWIDNYRTERVL*KNELQCIFNPPAA 508 V F +HLE A LDT++ A IR RR P I SDNG NF G N ++ I +D+Y+ + + N+++ IFN P A Sbjct: 216 VDFAGPAVHLEVAYALDTDAFIHAFIRFMGRRGKPLMILSDNGTNFVGATNEVQEIKVDSYKFNKFMSGNDIRWIFNSPLA 296
BLAST of SMED30024068 vs. Planmine SMEST
Match: SMESG000043416.1 (SMESG000043416.1) HSP 1 Score: 64.6994 bits (156), Expect = 2.930e-20 Identity = 35/87 (40.23%), Postives = 55/87 (63.22%), Query Frame = -3 Query: 567 LIFSDRSWISKLIVKDFHKKKHQYAGV------LDKIFWLLRAIQIIQVIENQRVGYCRQKAKGST*LMAPRSQFRFAKPLRAFAQM 809 +I SD SWI++LI+K+ H K YAGV L++ +W++ A ++I+ + R+KAKG +MAP FRF +PL+AFA++ Sbjct: 127 IILSDESWIARLIIKELHVKNKHYAGVNHTLGKLNRKYWVVCARKLIKKCIKRCFECRRRKAKGMEQIMAPLPSFRFQEPLQAFARI 213 HSP 2 Score: 51.9878 bits (123), Expect = 2.930e-20 Identity = 33/81 (40.74%), Postives = 46/81 (56.79%), Query Frame = -1 Query: 266 VCFKSKELHLE*ATGLDTESSXXXXXXXXXXXRMPKTISSDNGKNFEGTNNHMENIWIDNYRTERVL*KNELQCIFNPPAA 508 V F +HLE A LDT++ A IR RR P I SDNG NF G N ++ I +D+Y+ + + N+++ IFN P A Sbjct: 215 VDFAGPAVHLEVAYALDTDAFIHAFIRFMGRRGKPLMILSDNGTNFVGATNEVQEIKVDSYKFNKFMSGNDIRWIFNSPLA 295
BLAST of SMED30024068 vs. Planmine SMEST
Match: SMESG000041327.1 (SMESG000041327.1) HSP 1 Score: 65.0846 bits (157), Expect = 3.539e-12 Identity = 41/94 (43.62%), Postives = 58/94 (61.70%), Query Frame = -1 Query: 266 NQSRQR*RY--LFVCFKSKELHLE*ATGLDTESSXXXXXXXXXXXRMPKTISSDNGKNFEGTNNHMENIWIDNYRTERVL*KNELQCIFNPPAA 541 ++RQ+ RY LF C +++ +HLE A LDT + RA IR RR P I SDNG NF G N ++ I +D+YR + + N+++ IFNPP A Sbjct: 71 GKTRQK-RYMCLFTCLQTRAVHLEVAYALDTNAFIRAFIRFMGRRGKPLMILSDNGTNFVGATNEVQEIKVDSYRFNKFMSGNDIRWIFNPPLA 163 The following BLAST results are available for this feature:
BLAST of SMED30024068 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30024068 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30024068 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30024068 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30024068 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30024068 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30024068 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30024068 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30024068 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30024068 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30024068 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30024068 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30024068 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30024068 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30024068 ID=SMED30024068|Name=SMED30024068|organism=Schmidtea mediterranea sexual|type=transcript|length=811bpback to top Annotated Terms
The following terms have been associated with this transcript:
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