LAS1-like protein

Overview
NameLAS1-like protein
Smed IDSMED30024065
Length (bp)1296
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of LAS1-like protein (SMED30024065) t-SNE clustered cells

Violin plots show distribution of expression levels for LAS1-like protein (SMED30024065) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of LAS1-like protein (SMED30024065) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for LAS1-like protein (SMED30024065) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30024065

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30024065SMESG000074249.1 SmedASXL_014000SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30024065SMESG000074249.1 dd_Smed_v4_10589_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
zeta neoblastSMED30024065SMESG000074249.1 dd_Smed_v4_10589_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of LAS1-like protein vs. TrEMBL
Match: A0A430QTS0 (Ribosomal biogenesis protein LAS1 OS=Schistosoma bovis OX=6184 GN=DC041_0008574 PE=4 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 2.018e-15
Identity = 88/358 (24.58%), Postives = 166/358 (46.37%), Query Frame = 2 
Query:   53 NNRFQVYADIKEYLHVAKLIISNDSQSHVKALSYLHTWCERKPENKIPRSAYITFCLLKAFYEEKFTMALSFALSKFISSECKEEEDKNNFNHKIPIYSTAKNIKLPAYMIKLRHDIIHNDDNVSYEQLADAYNDAIKWLIISFWIPQLDYVEGIYCNLDSI----SEILDEYV--LINKEKSIKKD-DNYFKKVNEFIDGISINNFFKVLLTDKYLFNKEFDDLDNSKNILFELWYPMIEYLDALNQIDMLIESVLNSLGSTNYEISTMDIIR-KWSMCLLEACTVKRKAKKNQLKISFNPINTRNFNWPNILCKFIKQSLCKENMDILKKLISVHQPAIPKEKQEKIRSTLDEICS 1102
            + R   + D  E+  VA+ + S ++     A   +  W  R P +KIPRS   T+ LL A++E   + +++ AL +F+S    E +D+   N  +PI S A+   LP+++  LR+DI H     S + L  A+  ++K+L   FW   ++Y E     LD +    S  L +YV  L+ +EK+ + D    F+  + ++    I N         ++      ++  ++ I  +  +  + Y   + ++      VL  +     + S  D IR +W +  ++A     K + ++  I  + ++  + +W   L   +K  +C +  D+  +L+ +  P IP+EK E I S +D  C 
Sbjct:    4 DKRIVPWVDASEFSEVARNVSSCETYKLRLAWETIKIWLCRMPVSKIPRSIICTYELLNAYFENS-SQSMALALMRFVSLLSSESQDRERPNFALPILSLARVAGLPSWLADLRNDIAHGIIP-STDTLESAFRWSLKYL-SEFWASNVNYNEEQLIELDGLLKCQSLALTKYVENLLQEEKTTQLDVKEVFRNRSTYVCFPLIVNTICSYGCAMFVGGSPPCNIVQNQAIKLKPLFSTMLYHKLVGEL------VLQFILGLRDDHSVDDDIRLEWCIAWIKAI----KCRGSEKSILRDYVDGLSLDWRKALNHILKH-MCNKYRDLFMELLIIRDPPIPQEKFEVIMSHIDVFCG 347
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BLAST of LAS1-like protein vs. TrEMBL
Match: A0A095A8K1 (Pre-rRNA-processing protein las1 OS=Schistosoma haematobium OX=6185 GN=MS3_10686 PE=4 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 3.317e-15
Identity = 90/358 (25.14%), Postives = 162/358 (45.25%), Query Frame = 2 
Query:   53 NNRFQVYADIKEYLHVAKLIISNDSQSHVKALSYLHTWCERKPENKIPRSAYITFCLLKAFYEEKFTMALSFALSKFISSECKEEEDKNNFNHKIPIYSTAKNIKLPAYMIKLRHDIIHNDDNVSYEQLADAYNDAIKWLIISFWIPQLDYVEGIYCNLDSI----SEILDEYV--LINKEKSIKKDDNYFKKVNEFIDGISINNFFKVLLTDKYLFN-KEFDDLDNSKNILFELWYPMIEYLDALNQIDMLIESVLN-SLGSTNYEISTMDIIRKWSMCLLEACTVKRKAKKNQLKISFNPINTRNFNWPNILCKFIKQSLCKENMDILKKLISVHQPAIPKEKQEKIRSTLDEICS 1102
            + R   + D  E+  VA+ + S ++     A   +  W  R P +KIPRS   T+ LL A++E   + +++ AL +F+S    E +D+   N  +PI S A+   LP+++  LR+DI H     S + L  A+  ++K+L   FW   ++Y E  +  LD +    S  L +YV  L+ +EK+ + D      V E     S    F +++     +    F       NI+      +      +    ++ E VL   LG  +      DI  +W +  ++A     K + ++  I  + ++  + +W   L   +K  +C +  D+  +L+ +  P IP++K E I S +D  C 
Sbjct:    4 DKRIVPWVDASEFSDVARNVSSCETYKLRLAWETIKIWLCRMPVSKIPRSIICTYELLNAYFENS-SQSMALALMRFVSLLSSESQDRERPNFALPILSLARVAGLPSWLADLRNDIAHGIIP-STDTLESAFRWSLKYL-SEFWASNVNYNEEQFIELDGLLKCQSLALTKYVENLLQEEKTTQLD------VKEVFRNRSTYACFPLIVNTICSYGCAMFVGGSPPCNIVQNQAIKLKPLFSTMLYHKLVGELVLQFILGLRDDHTVDDDIRLEWCIAWIKAI----KCRGSEKSILRDYVDGLSLDWRKALNHILKH-MCNKYRDLFMRLLIIRDPPIPQDKFEVIMSHIDVFCG 347
[Close]
BLAST of LAS1-like protein vs. TrEMBL
Match: A0A183JIZ2 (Uncharacterized protein OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS2667 PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 4.893e-15
Identity = 90/358 (25.14%), Postives = 164/358 (45.81%), Query Frame = 2 
Query:   53 NNRFQVYADIKEYLHVAKLIISNDSQSHVKALSYLHTWCERKPENKIPRSAYITFCLLKAFYEEKFTMALSFALSKFISSECKEEEDKNNFNHKIPIYSTAKNIKLPAYMIKLRHDIIHNDDNVSYEQLADAYNDAIKWLIISFWIPQLDYVEGIYCNLDSI----SEILDEYV--LINKEKSIKKDDNYFKKVNEFIDGISINNFFKVLLTDKYLFN-KEFDDLDNSKNILFELWYPMIEYLDALNQIDMLIESVLNSLGSTNYEISTMDIIR-KWSMCLLEACTVKRKAKKNQLKISFNPINTRNFNWPNILCKFIKQSLCKENMDILKKLISVHQPAIPKEKQEKIRSTLDEICS 1102
            + R   + D  E+  VA+ + S ++     A   +  W  R P +KIPRS   T+ LL A++E   + +++ AL +F+S    E +D+   N  +PI S A+   LP+++  LR+DI H     S + L  A+  ++K+L   FW   ++Y E  +  LD +    S  L +YV  L+ +EK+ + D      V E     S    F +++     +    F       NI+ +    +      +    ++ E VL  +     + S  D IR +W +  ++A     K + ++  I  + ++  + +W   L   +K  +C +  D+  +L+ +  P IP++K E I S +D  C 
Sbjct:    4 DKRIVPWVDDSEFSDVARNVSSCETYKLRLAWETIKIWLCRMPVSKIPRSIICTYELLNAYFENS-SQSMALALMRFVSLLSSESQDRERPNFALPILSLARVAGLPSWLADLRNDIAHGIIP-STDTLESAFRWSLKYL-SEFWASNVNYNEEQFIELDGLLKCQSLALTKYVENLLQEEKTTQLD------VKEVFRNRSTYACFPLIVNTICSYGCAMFVGGSPPCNIVQDQAIKLKPLFSTMLYHKLVGELVLQFILGLRDDHSVDDDIRLEWCIAWIKAI----KCRGSEKSILRDYVDGLSLDWRKALNHILKH-MCNKYRDLFMELLIIRDPPIPQDKFEVIMSHIDVFCG 347
[Close]
BLAST of LAS1-like protein vs. TrEMBL
Match: A0A183RB41 (Uncharacterized protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 4.419e-14
Identity = 86/349 (24.64%), Postives = 153/349 (43.84%), Query Frame = 2 
Query:   71 YADIKEYLHVAKLIISNDSQSHVKALSYLHTWCERKPENKIPRSAYITFCLLKAFYEEKFTMALSFALSKFISSECKEEEDKNNFNHKIPIYSTAKNIKLPAYMIKLRHDIIHNDDNVSYEQLADAYNDAIKWLIISFWIPQLDYVEGIYCNLDSISEILDEYVLINKEKSIKKDDNYFKKVNEFIDGISINNFFKVLLT-----DKYLFNKEFDDLDNSKNILFELWYPMIEYLDALNQIDMLIESVLNSLGSTNYEISTMDIIRKWSMCLLEACTVKRKAKKNQLKISFNPINTRNFNWPNILCKFIKQSLCKENMDILKKLISVHQPAIPKEKQEKIRSTLDEICS 1102
            + D  E+  VA  + S ++     A   +  W  R P +KIPRS   T+ LL A++E   + +++ AL +F+S    E +D+   N  +PI S A+   LP+++  LR+DI H     S + L  A+  ++K+L   FW   ++Y E     LD   +     ++   E  ++ D      + E     S    F +++         LF  E    +  +N   +L  P+   +     +  L+   +  LG  N      DI  +W +  +EA   +   K     I  + ++  +F+W   L   I   +C +  D+L +L+ +  P IP++K + I S +D  C 
Sbjct:    7 WVDASEFSDVANNVSSCETYKLRLAWETIKIWLCRMPVSKIPRSIVCTYELLNAYFENS-SQSMALALMRFVSLLSSESQDRERPNFALPILSLARVAGLPSWLADLRNDIAHGIIP-STDTLESAFWWSLKYL-SEFWASNVNYNEERMVELDGFLKCQSLTLIKYIESLLQGDKTSQLNMKEVFSSRSTYACFPLIINTICSYGCNLFTGESPLCNIVQNQAIKL-KPLFSTMLYHKLVGELVLQFI--LGLRNDRSLDDDIRLEWCVAWIEAIRCRVSGK----SILCDYVDGLSFDWRKAL-DHILNHMCDKYRDLLMELLIIRDPPIPQDKLKVIMSHIDVFCG 344
[Close]
BLAST of LAS1-like protein vs. TrEMBL
Match: A0A183LWC7 (Uncharacterized protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS8101 PE=4 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 6.171e-14
Identity = 88/364 (24.18%), Postives = 167/364 (45.88%), Query Frame = 2 
Query:   53 NNRFQVYADIKEYLHVAKLIISNDSQSHVKALSYLHTWCERKPENKIPRSAYITFCLLKAFYEEKFTMALSFALSKFISSECKEEEDKNNFNHKIPIYSTAKNIKLPAYMIKLRHDIIHNDDNVSYEQLADAYNDAIKWLIISFWIPQLDYVEGIYCNLDSI----SEILDEYV--LINKEKSIKKDDNYFKKVNEFIDGISINNFFKVLLTD-------KYLFNKEFDDLDNSKNILFELWYPMIEYLDALNQIDMLIESVLNSLGSTNYEISTMDIIR-KWSMCLLEACTVKRKAKKNQLKISFNPINTRNFNWPNILCKFIKQSLCKENMDILKKLISVHQPAIPKEKQEKIRSTLDEICS 1102
            + R   + D  E+  VA+ + S ++     A   +  W  R P +KIPRS   T+ LL A++E   + +++ AL +F+S    E +D+   N  +PI S A+   LP+++  LR+DI H     S + L  A+  ++K+L   FW   +++ E  +  LD +    S  L +YV  L+  EK+ + D      V E     SI   F +++          ++       +  ++ I  +  +  + Y   + ++      VL  +     + S  D IR +W +  ++A     K ++++  I  + ++  + +W   L   +K  +C +  D+  +L+ +  P IP++K E I S +D  C 
Sbjct:    4 DKRIVPWVDASEFSDVARNVSSCETYRLRLAWETIKIWLCRMPVSKIPRSIICTYELLNAYFENS-SQSMALALMRFVSLLSSESQDRERPNFALPILSLARVAGLPSWLADLRNDIAHGIIP-STDTLESAFRWSLKYL-SEFWASNVNHNEEQFVELDGLLKCQSLALTKYVENLLQGEKTSQLD------VKEVFRNRSIYACFPLIVNTICSYGCAVFVGGSPLCIIVQNQAIKLKPLFSTMLYHKLVGEL------VLQFILGLRDDNSVDDDIRLEWCIAWIKAI----KCRESEKSILCDYVDGLSLDWRLALNHILKH-MCNKYRDLFMELLIIRDPPIPQDKFEVIMSHIDVFCG 347
[Close]
BLAST of LAS1-like protein vs. Planmine SMEST
Match: SMESG000074249.1 (SMESG000074249.1)

HSP 1 Score: 753.051 bits (1943), Expect = 0.000e+0
Identity = 372/373 (99.73%), Postives = 372/373 (99.73%), Query Frame = 2 
Query:   41 MQSKNNRFQVYADIKEYLHVAKLIISNDSQSHVKALSYLHTWCERKPENKIPRSAYITFCLLKAFYEEKFTMALSFALSKFISSECKEEEDKNNFNHKIPIYSTAKNIKLPAYMIKLRHDIIHNDDNVSYEQLADAYNDAIKWLIISFWIPQLDYVEGIYCNLDSISEILDEYVLINKEKSIKKDDNYFKKVNEFIDGISINNFFKVLLTDKYLFNKEFDDLDNSKNILFELWYPMIEYLDALNQIDMLIESVLNSLGSTNYEISTMDIIRKWSMCLLEACTVKRKAKKNQLKISFNPINTRNFNWPNILCKFIKQSLCKENMDILKKLISVHQPAIPKEKQEKIRSTLDEICSNGCIDVDFSSKSTEHSFCF 1159
            MQSKNNRFQVYADIKEYLHVAKLIISNDSQSHVKALSYLHTWCERKPENKIPRSAYITFCLLKAFYEEKFTMALSFALSKFISSECKEEEDKNNFNHKIPIYSTAKNIKLPAYMIKLRHDIIHNDDNVSYEQLADAYNDAIKWLIISFWIPQLDYVEGIYCNLDSISEILDEYVLINKEKSIKKDDNYFKKVNEFIDGISINNFFKVLLTDKYLFNKEFDDLDNSKNILFELWYPMIEYLDALNQID LIESVLNSLGSTNYEISTMDIIRKWSMCLLEACTVKRKAKKNQLKISFNPINTRNFNWPNILCKFIKQSLCKENMDILKKLISVHQPAIPKEKQEKIRSTLDEICSNGCIDVDFSSKSTEHSFCF
Sbjct:    1 MQSKNNRFQVYADIKEYLHVAKLIISNDSQSHVKALSYLHTWCERKPENKIPRSAYITFCLLKAFYEEKFTMALSFALSKFISSECKEEEDKNNFNHKIPIYSTAKNIKLPAYMIKLRHDIIHNDDNVSYEQLADAYNDAIKWLIISFWIPQLDYVEGIYCNLDSISEILDEYVLINKEKSIKKDDNYFKKVNEFIDGISINNFFKVLLTDKYLFNKEFDDLDNSKNILFELWYPMIEYLDALNQIDTLIESVLNSLGSTNYEISTMDIIRKWSMCLLEACTVKRKAKKNQLKISFNPINTRNFNWPNILCKFIKQSLCKENMDILKKLISVHQPAIPKEKQEKIRSTLDEICSNGCIDVDFSSKSTEHSFCF 373
The following BLAST results are available for this feature:
BLAST of LAS1-like protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of LAS1-like protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of LAS1-like protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of LAS1-like protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of LAS1-like protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of LAS1-like protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of LAS1-like protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of LAS1-like protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A430QTS02.018e-1524.58Ribosomal biogenesis protein LAS1 OS=Schistosoma b... [more]
A0A095A8K13.317e-1525.14Pre-rRNA-processing protein las1 OS=Schistosoma ha... [more]
A0A183JIZ24.893e-1525.14Uncharacterized protein OS=Schistosoma curassoni O... [more]
A0A183RB414.419e-1424.64Uncharacterized protein OS=Schistosoma rodhaini OX... [more]
A0A183LWC76.171e-1424.18Uncharacterized protein OS=Schistosoma margrebowie... [more]
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BLAST of LAS1-like protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of LAS1-like protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of LAS1-like protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of LAS1-like protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of LAS1-like protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of LAS1-like protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000074249.10.000e+099.73SMESG000074249.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024065 ID=SMED30024065|Name=LAS1-like protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1296bp
AATACGACTTTTCTCATTTTTTAAGCATTTTTAACTTTTTATGCAATCAA
AAAATAATCGTTTTCAAGTGTATGCTGATATTAAAGAATATTTGCATGTG
GCAAAATTAATAATTTCTAACGACTCACAATCGCATGTCAAGGCATTATC
TTATCTTCATACGTGGTGTGAAAGGAAACCGGAAAATAAAATACCAAGGT
CAGCATATATTACTTTTTGCTTATTAAAGGCATTTTATGAAGAAAAATTT
ACCATGGCATTATCATTTGCCTTATCAAAATTTATTTCTAGTGAATGTAA
GGAAGAAGAAGACAAAAATAATTTCAATCATAAAATTCCTATTTATTCAA
CTGCTAAAAATATTAAATTGCCAGCATATATGATTAAATTAAGACATGAT
ATCATCCATAATGATGATAATGTATCATATGAGCAATTAGCAGATGCTTA
TAATGATGCTATAAAATGGTTAATTATTAGTTTTTGGATTCCACAGTTAG
ATTATGTCGAAGGAATATATTGTAATTTGGATTCTATATCAGAAATATTA
GATGAATATGTTTTGATCAATAAAGAAAAATCAATTAAAAAAGATGACAA
TTATTTTAAAAAAGTTAATGAATTTATTGATGGAATCTCGATAAATAATT
TTTTTAAAGTTTTATTGACAGATAAATATTTATTTAACAAAGAATTCGAT
GATTTAGATAATTCAAAAAACATCCTATTCGAATTGTGGTATCCAATGAT
AGAATACTTGGATGCATTGAATCAAATAGATATGCTTATTGAATCAGTTT
TAAATTCTTTGGGATCAACAAATTATGAAATTTCAACTATGGATATTATT
CGAAAATGGTCTATGTGTTTATTGGAAGCATGTACAGTTAAAAGAAAAGC
TAAAAAAAATCAGTTAAAAATATCTTTTAATCCCATAAACACAAGAAATT
TTAATTGGCCTAATATTCTCTGTAAATTTATTAAGCAATCTCTATGCAAA
GAAAATATGGATATTTTGAAAAAATTAATCTCTGTTCATCAGCCAGCTAT
TCCAAAAGAAAAACAAGAAAAAATCAGATCTACATTAGATGAAATTTGTT
CTAATGGATGTATCGATGTCGACTTCTCAAGTAAATCTACAGAGCACTCG
TTTTGTTTCTAACCTTTGCAGTGTTATTAATAAATTTGTTTTGAGATTTA
GTTTTATTTCACTCATTCTTGCATTTTAGATTTTGATTCTATTGCATTGT
TTTTATGACATAAACATACTTTTTCCATGTTTTTATTTTTTTCTTG
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protein sequence of SMED30024065-orf-1

>SMED30024065-orf-1 ID=SMED30024065-orf-1|Name=SMED30024065-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=374bp
MQSKNNRFQVYADIKEYLHVAKLIISNDSQSHVKALSYLHTWCERKPENK
IPRSAYITFCLLKAFYEEKFTMALSFALSKFISSECKEEEDKNNFNHKIP
IYSTAKNIKLPAYMIKLRHDIIHNDDNVSYEQLADAYNDAIKWLIISFWI
PQLDYVEGIYCNLDSISEILDEYVLINKEKSIKKDDNYFKKVNEFIDGIS
INNFFKVLLTDKYLFNKEFDDLDNSKNILFELWYPMIEYLDALNQIDMLI
ESVLNSLGSTNYEISTMDIIRKWSMCLLEACTVKRKAKKNQLKISFNPIN
TRNFNWPNILCKFIKQSLCKENMDILKKLISVHQPAIPKEKQEKIRSTLD
EICSNGCIDVDFSSKSTEHSFCF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0004519endonuclease activity
GO:0003674molecular_function
Vocabulary: biological process
TermDefinition
GO:0090305nucleic acid phosphodiester bond hydrolysis
GO:0006351transcription, DNA-templated
GO:0006355regulation of transcription, DNA-templated
GO:0006364rRNA processing
GO:0030702chromatin silencing at centromere
Vocabulary: cellular component
TermDefinition
GO:0090730Las1 complex
GO:0000775chromosome, centromeric region
GO:0000922spindle pole
GO:0005634nucleus
GO:0005635nuclear envelope
GO:0005694chromosome
GO:0005720nuclear heterochromatin
GO:0005737cytoplasm
GO:0005856cytoskeleton
GO:0044732mitotic spindle pole body
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR007174Las1
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007174Las1PFAMPF04031Las1coord: 11..150
e-value: 6.9E-16
score: 58.7
IPR007174Las1PANTHERPTHR15002UNCHARACTERIZEDcoord: 2..348