chromosome transmission fidelity protein 8 homolog

Overview
Namechromosome transmission fidelity protein 8 homolog
Smed IDSMED30024050
Length (bp)541
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of chromosome transmission fidelity protein 8 homolog (SMED30024050) t-SNE clustered cells

Violin plots show distribution of expression levels for chromosome transmission fidelity protein 8 homolog (SMED30024050) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of chromosome transmission fidelity protein 8 homolog (SMED30024050) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for chromosome transmission fidelity protein 8 homolog (SMED30024050) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30024050

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30024050SMESG000064421.1 SmedASXL_005001SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
zeta neoblastSMED30024050SMESG000064421.1 dd_Smed_v4_15527_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Human
Match: CHTF8 (chromosome transmission fidelity factor 8 [Source:HGNC Symbol;Acc:HGNC:24353])

HSP 1 Score: 82.4185 bits (202), Expect = 4.077e-20
Identity = 35/109 (32.11%), Postives = 64/109 (58.72%), Query Frame = 3 
Query:  144 AVNTDNLKENFIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNSEERDDTVKNFVVEAIVRRKLIFNTRPQPIITN 470
            +     L E  ++ELQG +E + S   +  + G L ++ +  P+L++GHH+L GKI++L++P  +  K         E   +T   ++V A+++ K++F TRP+PIIT+
Sbjct:    8 SARAGGLAEWVLMELQGEIEARYSTGLAGNLLGDLHYTTEGIPVLIVGHHILYGKIIHLEKPFAVLVKHTPGDQDCDELGRETGTRYLVTALIKDKILFKTRPKPIITS 116
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Human
Match: CHTF8 (chromosome transmission fidelity factor 8 [Source:HGNC Symbol;Acc:HGNC:24353])

HSP 1 Score: 82.4185 bits (202), Expect = 4.077e-20
Identity = 35/109 (32.11%), Postives = 64/109 (58.72%), Query Frame = 3 
Query:  144 AVNTDNLKENFIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNSEERDDTVKNFVVEAIVRRKLIFNTRPQPIITN 470
            +     L E  ++ELQG +E + S   +  + G L ++ +  P+L++GHH+L GKI++L++P  +  K         E   +T   ++V A+++ K++F TRP+PIIT+
Sbjct:    8 SARAGGLAEWVLMELQGEIEARYSTGLAGNLLGDLHYTTEGIPVLIVGHHILYGKIIHLEKPFAVLVKHTPGDQDCDELGRETGTRYLVTALIKDKILFKTRPKPIITS 116
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Human
Match: CHTF8 (chromosome transmission fidelity factor 8 [Source:HGNC Symbol;Acc:HGNC:24353])

HSP 1 Score: 46.595 bits (109), Expect = 4.208e-7
Identity = 21/60 (35.00%), Postives = 37/60 (61.67%), Query Frame = 3 
Query:  291 HVLLGKIVNLDEPLLITKKSVLKTSSNSEERDDTVKNFVVEAIVRRKLIFNTRPQPIITN 470
            H+L GKI++L++P  +  K         E   +T   ++V A+++ K++F TRP+PIIT+
Sbjct:    1 HILYGKIIHLEKPFAVLVKHTPGDQDCDELGRETGTRYLVTALIKDKILFKTRPKPIITS 60
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Celegans
Match: ctf-8 (Chromosome Transmission Fidelity factor homolog [Source:UniProtKB/TrEMBL;Acc:Q22664])

HSP 1 Score: 71.2478 bits (173), Expect = 2.655e-16
Identity = 35/103 (33.98%), Postives = 56/103 (54.37%), Query Frame = 3 
Query:  156 DNLKENFIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNSEERDDTVKNFVVEAIVRRKLIFNTRPQPII 464
            +   E   +EL G++  +D  SF ++  G + +       +VIG+  L GKI  +D PLL+ +KS      N E      KN  V A++R+KL+F TRP+P++
Sbjct:   10 EGTPEWMAIELHGAISPQDDGSFDSKTLGTICWGDHNNVYMVIGNQTLEGKISKIDRPLLVIQKSDKNHQENGE------KNATVNAVIRKKLVFKTRPRPLV 106
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Fly
Match: CG34001 (gene:FBgn0054001 transcript:FBtr0300947)

HSP 1 Score: 75.485 bits (184), Expect = 2.084e-17
Identity = 44/128 (34.38%), Postives = 68/128 (53.12%), Query Frame = 3 
Query:  177 IVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKS-------VLKTSSNSE-----------ER---DDTVKN---------FVVEAIVRRKLIFNTRPQPIITN 470
            I+ELQG LE++ +     Q  G L+++K  +P+L+IGHH+L G+   LD+P  + +KS       +L+TS  S+           ER   D T+           + V A+  +KLIF +RP+PII N
Sbjct:   17 IIELQGDLEVRSNQDMHGQFIGDLYYNKYGQPILIIGHHILQGREQKLDKPFAVLEKSKTNEGERLLETSMASQDVSMLNATTGAERTVLDHTIAQEHKSRQRTEYTVRAVCTKKLIFKSRPKPIIAN 144
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Fly
Match: CG34001 (gene:FBgn0054001 transcript:FBtr0334066)

HSP 1 Score: 50.8322 bits (120), Expect = 2.659e-8
Identity = 38/128 (29.69%), Postives = 59/128 (46.09%), Query Frame = 3 
Query:  177 IVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKS-------VLKTSSNSE-----------ER---DDTVKN---------FVVEAIVRRKLIFNTRPQPIITN 470
            I+ELQG LE++ +     Q  G L+++K  +P          G+   LD+P  + +KS       +L+TS  S+           ER   D T+           + V A+  +KLIF +RP+PII N
Sbjct:   17 IIELQGDLEVRSNQDMHGQFIGDLYYNKYGQP----------GREQKLDKPFAVLEKSKTNEGERLLETSMASQDVSMLNATTGAERTVLDHTIAQEHKSRQRTEYTVRAVCTKKLIFKSRPKPIIAN 134
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Zebrafish
Match: chtf8 (CTF8, chromosome transmission fidelity factor 8 homolog (S. cerevisiae) [Source:ZFIN;Acc:ZDB-GENE-050419-148])

HSP 1 Score: 80.8777 bits (198), Expect = 9.625e-20
Identity = 39/108 (36.11%), Postives = 67/108 (62.04%), Query Frame = 3 
Query:  156 DNLKENFIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPL-LITKKSVLKTSS--NSEERDDTVKNFVVEAIVRRKLIFNTRPQPIITN 470
            ++  E  +VELQG +  + +   +  + G L ++K+  P+L++GHH+L GK V L++P  ++ K S L   +  N E   +   ++ V A++++KLIF TRP+PIITN
Sbjct:   12 ESPAEWLLVELQGEMVSRQNTGLAGNLMGDLHYTKEGVPILIVGHHILYGKQVKLEKPFAVLMKHSGLPQDAEENMETNQEKPTSYTVSALIKKKLIFKTRPKPIITN 119
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Zebrafish
Match: chtf8 (CTF8, chromosome transmission fidelity factor 8 homolog (S. cerevisiae) [Source:ZFIN;Acc:ZDB-GENE-050419-148])

HSP 1 Score: 80.8777 bits (198), Expect = 9.625e-20
Identity = 39/108 (36.11%), Postives = 67/108 (62.04%), Query Frame = 3 
Query:  156 DNLKENFIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPL-LITKKSVLKTSS--NSEERDDTVKNFVVEAIVRRKLIFNTRPQPIITN 470
            ++  E  +VELQG +  + +   +  + G L ++K+  P+L++GHH+L GK V L++P  ++ K S L   +  N E   +   ++ V A++++KLIF TRP+PIITN
Sbjct:   12 ESPAEWLLVELQGEMVSRQNTGLAGNLMGDLHYTKEGVPILIVGHHILYGKQVKLEKPFAVLMKHSGLPQDAEENMETNQEKPTSYTVSALIKKKLIFKTRPKPIITN 119
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Zebrafish
Match: chtf8 (CTF8, chromosome transmission fidelity factor 8 homolog (S. cerevisiae) [Source:ZFIN;Acc:ZDB-GENE-050419-148])

HSP 1 Score: 80.8777 bits (198), Expect = 9.625e-20
Identity = 39/108 (36.11%), Postives = 67/108 (62.04%), Query Frame = 3 
Query:  156 DNLKENFIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPL-LITKKSVLKTSS--NSEERDDTVKNFVVEAIVRRKLIFNTRPQPIITN 470
            ++  E  +VELQG +  + +   +  + G L ++K+  P+L++GHH+L GK V L++P  ++ K S L   +  N E   +   ++ V A++++KLIF TRP+PIITN
Sbjct:   12 ESPAEWLLVELQGEMVSRQNTGLAGNLMGDLHYTKEGVPILIVGHHILYGKQVKLEKPFAVLMKHSGLPQDAEENMETNQEKPTSYTVSALIKKKLIFKTRPKPIITN 119
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Xenopus
Match: cbx6 (chromobox 6 [Source:Xenbase;Acc:XB-GENE-951161])

HSP 1 Score: 60.8474 bits (146), Expect = 6.734e-12
Identity = 29/78 (37.18%), Postives = 53/78 (67.95%), Query Frame = 3 
Query:  168 ENFI-VELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNS-EERDDTV 395
            E++I +ELQG +E +  +  + ++ G L ++K+  PLL++GHH+L GK+V LD+P  +    ++K+S +S +E D T+
Sbjct:   15 EDWILMELQGEIEARKQDGLAGKMMGDLHYTKEGVPLLLVGHHILYGKMVRLDKPFAV----LVKSSGDSLQEVDPTM 88
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Xenopus
Match: cbx6 (chromobox 6 [Source:Xenbase;Acc:XB-GENE-951161])

HSP 1 Score: 60.4622 bits (145), Expect = 7.588e-12
Identity = 29/78 (37.18%), Postives = 53/78 (67.95%), Query Frame = 3 
Query:  168 ENFI-VELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNS-EERDDTV 395
            E++I +ELQG +E +  +  + ++ G L ++K+  PLL++GHH+L GK+V LD+P  +    ++K+S +S +E D T+
Sbjct:   15 EDWILMELQGEIEARKQDGLAGKMMGDLHYTKEGVPLLLVGHHILYGKMVRLDKPFAV----LVKSSGDSLQEVDPTM 88
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Xenopus
Match: cbx6 (chromobox 6 [Source:Xenbase;Acc:XB-GENE-951161])

HSP 1 Score: 60.077 bits (144), Expect = 3.453e-11
Identity = 29/78 (37.18%), Postives = 53/78 (67.95%), Query Frame = 3 
Query:  168 ENFI-VELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNS-EERDDTV 395
            E++I +ELQG +E +  +  + ++ G L ++K+  PLL++GHH+L GK+V LD+P  +    ++K+S +S +E D T+
Sbjct:   15 EDWILMELQGEIEARKQDGLAGKMMGDLHYTKEGVPLLLVGHHILYGKMVRLDKPFAV----LVKSSGDSLQEVDPTM 88
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Mouse
Match: Chtf8 (CTF8, chromosome transmission fidelity factor 8 [Source:MGI Symbol;Acc:MGI:2443370])

HSP 1 Score: 83.9593 bits (206), Expect = 6.785e-21
Identity = 35/109 (32.11%), Postives = 63/109 (57.80%), Query Frame = 3 
Query:  144 AVNTDNLKENFIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNSEERDDTVKNFVVEAIVRRKLIFNTRPQPIITN 470
            +   + L E  ++ELQG +E + S   +  + G L ++ +  P+L++GHH+L GK ++L++P  +  K         E    T   ++V A+++ K++F TRP+PIITN
Sbjct:    8 STGAEGLAEWVLMELQGEIEARYSTGLAGNLLGDLHYTTEGIPVLIVGHHILYGKTIHLEKPFAVLVKHTPGKQDCDEPGRGTGTQYLVTALIKNKILFKTRPKPIITN 116
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Mouse
Match: Chtf8 (CTF8, chromosome transmission fidelity factor 8 [Source:MGI Symbol;Acc:MGI:2443370])

HSP 1 Score: 83.9593 bits (206), Expect = 6.785e-21
Identity = 35/109 (32.11%), Postives = 63/109 (57.80%), Query Frame = 3 
Query:  144 AVNTDNLKENFIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNSEERDDTVKNFVVEAIVRRKLIFNTRPQPIITN 470
            +   + L E  ++ELQG +E + S   +  + G L ++ +  P+L++GHH+L GK ++L++P  +  K         E    T   ++V A+++ K++F TRP+PIITN
Sbjct:    8 STGAEGLAEWVLMELQGEIEARYSTGLAGNLLGDLHYTTEGIPVLIVGHHILYGKTIHLEKPFAVLVKHTPGKQDCDEPGRGTGTQYLVTALIKNKILFKTRPKPIITN 116
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Mouse
Match: Chtf8 (CTF8, chromosome transmission fidelity factor 8 [Source:MGI Symbol;Acc:MGI:2443370])

HSP 1 Score: 78.5666 bits (192), Expect = 4.879e-19
Identity = 33/97 (34.02%), Postives = 58/97 (59.79%), Query Frame = 3 
Query:  180 VELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNSEERDDTVKNFVVEAIVRRKLIFNTRPQPIITN 470
            +ELQG +E + S   +  + G L ++ +  P+L++GHH+L GK ++L++P  +  K         E    T   ++V A+++ K++F TRP+PIITN
Sbjct:    1 MELQGEIEARYSTGLAGNLLGDLHYTTEGIPVLIVGHHILYGKTIHLEKPFAVLVKHTPGKQDCDEPGRGTGTQYLVTALIKNKILFKTRPKPIITN 97
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Mouse
Match: Chtf8 (CTF8, chromosome transmission fidelity factor 8 [Source:MGI Symbol;Acc:MGI:2443370])

HSP 1 Score: 78.5666 bits (192), Expect = 4.879e-19
Identity = 33/97 (34.02%), Postives = 58/97 (59.79%), Query Frame = 3 
Query:  180 VELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNSEERDDTVKNFVVEAIVRRKLIFNTRPQPIITN 470
            +ELQG +E + S   +  + G L ++ +  P+L++GHH+L GK ++L++P  +  K         E    T   ++V A+++ K++F TRP+PIITN
Sbjct:    1 MELQGEIEARYSTGLAGNLLGDLHYTTEGIPVLIVGHHILYGKTIHLEKPFAVLVKHTPGKQDCDEPGRGTGTQYLVTALIKNKILFKTRPKPIITN 97
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. UniProt/SwissProt
Match: sp|P0C6T1|CTF8_RAT (Chromosome transmission fidelity protein 8 homolog OS=Rattus norvegicus OX=10116 GN=Chtf8 PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.199e-20
Identity = 36/109 (33.03%), Postives = 65/109 (59.63%), Query Frame = 3 
Query:  144 AVNTDNLKENFIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNSEERDDTVKNFVVEAIVRRKLIFNTRPQPIITN 470
            +   + L E  ++ELQG +E + S   +  + G L ++ +  P+L++GHH+L GKI++L++P  +  K         E   +T   ++V A+++ K++F TRP+PIITN
Sbjct:    8 STGAEGLAEWVLMELQGEIEARYSTGLAGNLLGDLHYTTEGIPVLIVGHHILYGKIIHLEKPFAVLVKHTPGKQDCDELGRETGTQYLVTALIKNKILFKTRPKPIITN 116
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. UniProt/SwissProt
Match: sp|P0CG15|CTF8_MOUSE (Chromosome transmission fidelity protein 8 homolog OS=Mus musculus OX=10090 GN=Chtf8 PE=3 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 4.746e-20
Identity = 35/109 (32.11%), Postives = 63/109 (57.80%), Query Frame = 3 
Query:  144 AVNTDNLKENFIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNSEERDDTVKNFVVEAIVRRKLIFNTRPQPIITN 470
            +   + L E  ++ELQG +E + S   +  + G L ++ +  P+L++GHH+L GK ++L++P  +  K         E    T   ++V A+++ K++F TRP+PIITN
Sbjct:    8 STGAEGLAEWVLMELQGEIEARYSTGLAGNLLGDLHYTTEGIPVLIVGHHILYGKTIHLEKPFAVLVKHTPGKQDCDEPGRGTGTQYLVTALIKNKILFKTRPKPIITN 116
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. UniProt/SwissProt
Match: sp|P0CG11|CTF8_BOVIN (Chromosome transmission fidelity protein 8 homolog OS=Bos taurus OX=9913 GN=CHTF8 PE=3 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 5.576e-20
Identity = 35/109 (32.11%), Postives = 64/109 (58.72%), Query Frame = 3 
Query:  144 AVNTDNLKENFIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNSEERDDTVKNFVVEAIVRRKLIFNTRPQPIITN 470
            +     L E  ++ELQG +E + S   +  + G L ++ +  P+L++GHH+L GKI++L++P  +  K         E   ++   ++V A+++ K++F TRP+PIITN
Sbjct:    8 SAGAGGLAEWVLMELQGEIEARHSTGLAGNLLGDLHYTTEGIPVLIVGHHILYGKIIHLEKPFAVLVKHTPGEQDCDELGCESGTRYLVTALIKNKILFKTRPKPIITN 116
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. UniProt/SwissProt
Match: sp|P0CG13|CTF8_HUMAN (Chromosome transmission fidelity protein 8 homolog OS=Homo sapiens OX=9606 GN=CHTF8 PE=1 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.958e-19
Identity = 35/109 (32.11%), Postives = 64/109 (58.72%), Query Frame = 3 
Query:  144 AVNTDNLKENFIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNSEERDDTVKNFVVEAIVRRKLIFNTRPQPIITN 470
            +     L E  ++ELQG +E + S   +  + G L ++ +  P+L++GHH+L GKI++L++P  +  K         E   +T   ++V A+++ K++F TRP+PIIT+
Sbjct:    8 SARAGGLAEWVLMELQGEIEARYSTGLAGNLLGDLHYTTEGIPVLIVGHHILYGKIIHLEKPFAVLVKHTPGDQDCDELGRETGTRYLVTALIKDKILFKTRPKPIITS 116
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. TrEMBL
Match: A0A1D2NCQ3 (Chromosome transmission fidelity protein 8 OS=Orchesella cincta OX=48709 GN=Ocin01_03839 PE=4 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 1.379e-21
Identity = 47/116 (40.52%), Postives = 74/116 (63.79%), Query Frame = 3 
Query:  123 MKCFLSCAVNTDNLKENFIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNSEERDDTVKNFVVEAIVRRKLIFNTRPQPIITN 470
            M+ F+    N D L+E  I+ELQG L+ K++ S + ++ G LF++KD  P  +IGHH+L+GK   L++PL + +K  ++T     E  D    +VV+AIV+RKL F  RP+PI+ +
Sbjct:   62 MQVFVQPFSNEDGLQEWGIIELQGDLDSKETGSLAGKLVGDLFYNKDGIPTFIIGHHILVGKTEKLEKPLAVLEKRRIRT-----EGIDDKTEYVVKAIVKRKLCFRERPKPIVGS 172
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. TrEMBL
Match: A1L2I2 (LOC100036855 protein OS=Xenopus laevis OX=8355 GN=chtf8 PE=1 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 2.231e-20
Identity = 41/103 (39.81%), Postives = 70/103 (67.96%), Query Frame = 3 
Query:  174 FIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNSEERDDTVKN----FVVEAIVRRKLIFNTRPQPIITN 470
             ++ELQG +E +  +  + +V G L ++K+  PLL++GHH+L GK+V LD+P  +    ++K+SS+ +E D T+      + V A++++K+IF TRP+PIITN
Sbjct:   18 LLMELQGQIEDRKQDGLTGKVMGDLHYTKEGVPLLIVGHHILYGKVVRLDKPFAV----LVKSSSSMQEADHTMTEASTRYQVTALIKKKIIFKTRPKPIITN 116
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. TrEMBL
Match: A0A1S3EVE8 (chromosome transmission fidelity protein 8 homolog OS=Dipodomys ordii OX=10020 GN=Chtf8 PE=4 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 2.406e-20
Identity = 38/109 (34.86%), Postives = 65/109 (59.63%), Query Frame = 3 
Query:  144 AVNTDNLKENFIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNSEERDDTVKNFVVEAIVRRKLIFNTRPQPIITN 470
            +     L E  ++ELQG +E + S   +  + G L ++ +  P+L++GHH+L GKI++L++P  +  K        SE R  T   ++V A+++ K++F TRP+PIITN
Sbjct:    8 SAGAGGLAEWVLMELQGEIEARYSTGLAGNLLGDLHYTTEGVPVLIVGHHILYGKIIHLEKPFAVLVKQASGEQDCSEPRHATGTQYLVTALIKNKILFKTRPKPIITN 116
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. TrEMBL
Match: V4B2K0 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_155672 PE=4 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 2.514e-20
Identity = 45/119 (37.82%), Postives = 70/119 (58.82%), Query Frame = 3 
Query:  123 MKCFLSCAVNTDNLKENFIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNSEERDDTVKN---FVVEAIVRRKLIFNTRPQPIITN 470
            ++ ++    +T N  E  I++LQG LE +     S +  G L F+K   P+ +IGHH+L GKIV L++P  + KK +    SN     D  K+   + VEA++R+K++F TRP+PII N
Sbjct:    2 VQLYVQIPGDTTNCPEWAIIDLQGDLETRHPVPLSGKFIGDLHFTKKDAPVFIIGHHILYGKIVELEKPFAVLKKMISTDESNDNMDTDCDKDNNHYEVEALIRKKILFKTRPKPIIAN 120
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BLAST of chromosome transmission fidelity protein 8 homolog vs. TrEMBL
Match: L5KUB0 (Chromosome transmission fidelity protein 8 like protein OS=Pteropus alecto OX=9402 GN=PAL_GLEAN10016276 PE=4 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 2.796e-20
Identity = 43/128 (33.59%), Postives = 76/128 (59.38%), Query Frame = 3 
Query:   87 YYKLLEKFTQNKMKCFLSCAVNTDNLKENFIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNSEERDDTVKNFVVEAIVRRKLIFNTRPQPIITN 470
            YY+LL K  +  ++  +S A     L E  ++ELQG +E + S   +  + G L ++ +  P+L++GHH+L GKI++L++P  +  K         E   +T   ++V A++++K++F TRP+PIITN
Sbjct:    6 YYQLLLKDGRLMVQIVVSSA-GAGGLAEWVLMELQGEIEARYSTGLAGNLLGDLHYTTEGIPVLIVGHHILYGKIIHLEKPFAVLVKHTPGEQDCDELGCETGTRYLVTALIKKKILFKTRPKPIITN 132
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BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Cavefish
Match: chtf8 (CTF8, chromosome transmission fidelity factor 8 homolog (S. cerevisiae) [Source:ZFIN;Acc:ZDB-GENE-050419-148])

HSP 1 Score: 49.6766 bits (117), Expect = 9.463e-8
Identity = 21/60 (35.00%), Postives = 37/60 (61.67%), Query Frame = 3 
Query:  168 ENFIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKK 347
            E  +VELQG +  + +   +  + G L ++K+  P+L++GHH+L GK V L++P  +  K
Sbjct:   16 EWLLVELQGEMLSRQNTGLAGNLMGDLHYTKEGVPVLIVGHHILYGKQVKLEKPFAVLMK 75
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BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Nematostella
Match: EDO32532 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SUS5])

HSP 1 Score: 83.9593 bits (206), Expect = 1.757e-21
Identity = 40/102 (39.22%), Postives = 62/102 (60.78%), Query Frame = 3 
Query:  159 NLKENFIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNSEERDDTVKNFVVEAIVRRKLIFNTRPQPII 464
             +++  IVELQG+LE +D  S   +  G L F     P L+IGHH+L GK+V LD+P  + +K        +++RD     + V A+V+RK++F +RP+PII
Sbjct:   11 TVEQWTIVELQGALETRDEVSLEGKNIGDLHFDSMGVPQLIIGHHLLTGKVVVLDKPYAVMRKVTKGDPMETDQRDTAETKYDVVALVKRKIVFKSRPKPII 112
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BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Medaka
Match: chtf8 (chromosome transmission fidelity factor 8 [Source:NCBI gene;Acc:101165976])

HSP 1 Score: 90.5077 bits (223), Expect = 1.496e-23
Identity = 44/124 (35.48%), Postives = 78/124 (62.90%), Query Frame = 3 
Query:  123 MKCFLSCAVNTDNLKENFIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNSEE-RDDTVK-------NFVVEAIVRRKLIFNTRPQPIITN 470
            ++ F+  +V+     E  +VELQG +  + ++  +  V G L ++K+  P+L++GHH+L GK V LD+P  +    ++K S +S++  DD ++       ++ V+A+++RKLIF TRP+PIITN
Sbjct:    2 VQIFVRSSVDGGGPSEWLLVELQGEMVSRHNSGLAGNVMGDLMYTKEGVPVLIVGHHILYGKQVKLDKPFAV----LMKHSGHSQDSHDDAMETDQRDSTSYSVKALIKRKLIFKTRPKPIITN 121
[Close]
BLAST of chromosome transmission fidelity protein 8 homolog vs. Planmine SMEST
Match: SMESG000064421.1 (SMESG000064421.1)

HSP 1 Score: 231.876 bits (590), Expect = 2.949e-79
Identity = 115/116 (99.14%), Postives = 116/116 (100.00%), Query Frame = 3 
Query:  123 MKCFLSCAVNTDNLKENFIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNSEERDDTVKNFVVEAIVRRKLIFNTRPQPIITN 470
            MKCFLSCAVNTDNLKENFIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNSEERDDTVKNFVVEAIVR+KLIFNTRPQPIITN
Sbjct:    1 MKCFLSCAVNTDNLKENFIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKPLLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNSEERDDTVKNFVVEAIVRQKLIFNTRPQPIITN 116
The following BLAST results are available for this feature:
BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
CHTF84.077e-2032.11chromosome transmission fidelity factor 8 [Source:... [more]
CHTF84.077e-2032.11chromosome transmission fidelity factor 8 [Source:... [more]
CHTF84.208e-735.00chromosome transmission fidelity factor 8 [Source:... [more]
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BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ctf-82.655e-1633.98Chromosome Transmission Fidelity factor homolog [... [more]
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BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CG340012.084e-1734.38gene:FBgn0054001 transcript:FBtr0300947[more]
CG340012.659e-829.69gene:FBgn0054001 transcript:FBtr0334066[more]
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BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
chtf89.625e-2036.11CTF8, chromosome transmission fidelity factor 8 ho... [more]
chtf89.625e-2036.11CTF8, chromosome transmission fidelity factor 8 ho... [more]
chtf89.625e-2036.11CTF8, chromosome transmission fidelity factor 8 ho... [more]
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BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
cbx66.734e-1237.18chromobox 6 [Source:Xenbase;Acc:XB-GENE-951161][more]
cbx67.588e-1237.18chromobox 6 [Source:Xenbase;Acc:XB-GENE-951161][more]
cbx63.453e-1137.18chromobox 6 [Source:Xenbase;Acc:XB-GENE-951161][more]
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BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Chtf86.785e-2132.11CTF8, chromosome transmission fidelity factor 8 [S... [more]
Chtf86.785e-2132.11CTF8, chromosome transmission fidelity factor 8 [S... [more]
Chtf84.879e-1934.02CTF8, chromosome transmission fidelity factor 8 [S... [more]
Chtf84.879e-1934.02CTF8, chromosome transmission fidelity factor 8 [S... [more]
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BLAST of chromosome transmission fidelity protein 8 homolog vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 4
Match NameE-valueIdentityDescription
sp|P0C6T1|CTF8_RAT1.199e-2033.03Chromosome transmission fidelity protein 8 homolog... [more]
sp|P0CG15|CTF8_MOUSE4.746e-2032.11Chromosome transmission fidelity protein 8 homolog... [more]
sp|P0CG11|CTF8_BOVIN5.576e-2032.11Chromosome transmission fidelity protein 8 homolog... [more]
sp|P0CG13|CTF8_HUMAN1.958e-1932.11Chromosome transmission fidelity protein 8 homolog... [more]
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BLAST of chromosome transmission fidelity protein 8 homolog vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1D2NCQ31.379e-2140.52Chromosome transmission fidelity protein 8 OS=Orch... [more]
A1L2I22.231e-2039.81LOC100036855 protein OS=Xenopus laevis OX=8355 GN=... [more]
A0A1S3EVE82.406e-2034.86chromosome transmission fidelity protein 8 homolog... [more]
V4B2K02.514e-2037.82Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
L5KUB02.796e-2033.59Chromosome transmission fidelity protein 8 like pr... [more]
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BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
chtf89.463e-835.00CTF8, chromosome transmission fidelity factor 8 ho... [more]
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BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO325321.757e-2139.22Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of chromosome transmission fidelity protein 8 homolog vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
chtf81.496e-2335.48chromosome transmission fidelity factor 8 [Source:... [more]
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BLAST of chromosome transmission fidelity protein 8 homolog vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000064421.12.949e-7999.14SMESG000064421.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30024050 ID=SMED30024050|Name=chromosome transmission fidelity protein 8 homolog|organism=Schmidtea mediterranea sexual|type=transcript|length=541bp
CATTAATTCAACTAGTTTATTTTTAAAATATTTTTGCATCTTTGAAATTT
TTTAATATTCTTAATATTTAATAAATTTTTCTTTTCTATTATAAATTATT
GGAGAAATTCACACAAAATAAAATGAAATGTTTTCTTAGTTGTGCTGTCA
ATACTGATAATCTGAAAGAAAATTTTATTGTTGAGTTGCAAGGAAGCTTA
GAGATTAAGGATAGTAATAGTTTTTCAAATCAAGTTTTTGGTCATTTGTT
CTTTTCTAAAGATAAAAAACCATTATTAGTTATTGGTCATCATGTACTCC
TTGGTAAAATCGTTAATTTAGATGAACCCTTGTTGATTACAAAGAAGTCG
GTATTAAAAACTTCCAGCAACAGTGAAGAAAGGGATGATACAGTTAAGAA
TTTTGTTGTTGAAGCCATTGTCAGACGAAAATTAATTTTCAATACTCGGC
CTCAGCCGATAATTACTAATGTTGCGAAAAAAGTGTAGAAATGAGTTATT
GTTAATGATAAATTGTTAATTAGAATTTTCGTTTGTTTTTG
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protein sequence of SMED30024050-orf-1

>SMED30024050-orf-1 ID=SMED30024050-orf-1|Name=SMED30024050-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=122bp
MKCFLSCAVNTDNLKENFIVELQGSLEIKDSNSFSNQVFGHLFFSKDKKP
LLVIGHHVLLGKIVNLDEPLLITKKSVLKTSSNSEERDDTVKNFVVEAIV
RRKLIFNTRPQPIITNVAKKV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR018607Ctf8
Vocabulary: biological process
TermDefinition
GO:0007064mitotic sister chromatid cohesion
Vocabulary: cellular component
TermDefinition
GO:0031390Ctf18 RFC-like complex
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018607Chromosome transmission fidelity protein 8PFAMPF09696Ctf8coord: 45..113
e-value: 5.2E-10
score: 39.8
NoneNo IPR availablePANTHERPTHR28605FAMILY NOT NAMEDcoord: 10..120